CN108753886B - Deer bone active peptide extraction device and extraction method - Google Patents
Deer bone active peptide extraction device and extraction method Download PDFInfo
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- CN108753886B CN108753886B CN201810621584.2A CN201810621584A CN108753886B CN 108753886 B CN108753886 B CN 108753886B CN 201810621584 A CN201810621584 A CN 201810621584A CN 108753886 B CN108753886 B CN 108753886B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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Abstract
A deer bone active peptide extraction device and an extraction method. The invention relates to a deer bone active peptide extraction device and an extraction method. Grinding rose box is connected to the siphunculus, sets up seal baffle II in the grinding rose box, and the left side wall of grinding the rose box is opened there is trapezoidal opening, and trapezoidal opening is passed in seal baffle II's left side, and the right side wall of grinding the rose box is inserted on seal baffle II's right side, and the left side of grinding the rose box sets up scraper blade I, and the right side of grinding the rose box sets up scraper blade II, and the steel cable is connected to seal baffle II's left side outer end, and the steel cable is through solid fixed ring connection linear electric motor II. The invention relates to a deer bone active peptide extraction device and an extraction method.
Description
Technical Field
The invention relates to a deer bone active peptide extraction device and an extraction method.
Background
Peptides are compounds in which two or more amino acids are linked by peptide bonds, which play important physiological roles in the human body and exert physiological functions, and active polypeptides are called active peptides, also called bioactive peptides or bioactive polypeptides. The active peptide is a general term of thousands of peptides (such as soybean peptide, deep-sea fish skin peptide, sea cucumber peptide and the like are one of the active peptides), plays a key role in the processes of growth and development, metabolism, diseases, aging and death of human bodies, and is the most important active substance in human bodies. It is because of the increase or decrease of the secretion in the body, the human beings have a period from childhood, adult, and the elderly to death. The injection of the active peptide breaks the life cycle, thereby achieving the miraculous effects of prolonging the life and effectively slowing down the aging. Deer bone active peptide extraction is divided into a plurality of steps, need be applied to a plurality of containers, but the conversion between individual container often needs artificial help, can reduce production efficiency to large-scale production, increases the personnel selection cost, and manual control time control is not accurate, causes the quality discrepancy, influences the quality.
Disclosure of Invention
The invention aims to provide a deer bone active peptide extraction device and an extraction method, which are used for solving the problems, all automation is realized except the final centrifugation step, the labor is reduced by controlling each step through a single chip microcomputer, and the production efficiency is increased.
The above purpose is realized by the following technical scheme:
a deer bone active peptide extraction element, funnel 1 sets up in coarse stirring case 2, coarse stirring case 2's left side inner wall on set up pivot I3, pivot I3 on connect sealed baffle I4, sealed baffle I4's right-hand member slide on slide 15, sealed baffle I4 on set up agitator 5, agitator 5's central bottom insert motor I6, motor I6's right side set up sealed L shaped plate 7, motor I6's bottom surface left side set up down tube I8, down tube I8's bottom connect vertical pole 9, vertical pole 9 set up the fuselage top surface at linear electric motor I10, down tube 8 pass through opening I11, coarse stirring case 2's right side bottom set up and be obtuse siphunculus 12 with the level,
the siphunculus 12 connect and grind rose box 13, grind rose box 13 in set up sealed baffle II 14, the left side wall of grinding rose box 13 open and to have trapezoidal opening 16, the left side of sealed baffle II 14 pass trapezoidal opening 16, the right side of sealed baffle II 14 insert the right side wall of grinding rose box 13, the left side of grinding rose box 13 set up scraper blade I17, the right side of grinding rose box 13 set up scraper blade II 18, the left side outer end of sealed baffle II 14 connect steel cable 19, steel cable 19 connect linear electric motor II 21's fuselage top surface through solid fixed ring 20,
the top end of the grinding and filtering box 13 is connected with a ring-shaped frame 26 through an 11-shaped support 25, an annular through groove of the ring-shaped frame 26 penetrates through an n-shaped frame 27, the top end of the n-shaped frame 27 is connected with a motor II 29 through a coupler I28, the motor II 29 is connected with the ring-shaped frame 26 through a frame 30, the coupler I28 is also vertically connected with an extension rod 31, the bottom end of the extension rod 31 penetrates through the top surface of the grinding and filtering box 13 and is connected with a group of grinders 32, the bottom end of the n-shaped frame 27 is respectively connected with one grinder 32 through an inclined rod II 33, a filter plate I34 is arranged below the sealing partition plate II 14, a filter plate II 35 is arranged below the filter plate I34, a filter plate III 36 is arranged below the filter plate II 35, and an access door 37 is arranged on the right side surfaces of the filter plate I34, the filter plate II 35 and the;
the bottom end of the grinding and filtering box 13 is a combined leak groove which comprises a conical leak groove 38 and an inclined through pipe 39, and the inclined through pipe 39 is inserted into a mixing box 40.
Deer bone active peptide extraction element, mixing box 40 in set up inlet tube 41, inlet tube 41 connect the water pump, mixing box 40 left side set up motor III 42, motor III 42 pass through shaft coupling II 43 and connect (mixing) shaft 44, (mixing) shaft 44 on set up stirring leaf 45, the right side of (mixing) shaft 44 set up PH liquid inlet tube 46 and PH test pen 47, mixing box 40's bottom set up left side pull door 48 and right side pull door 49, the place underframe 50 of left side pull door 48 and right side pull door 49 on slide static heating cabinet 51, static heating cabinet 51's the bottom plate intermediate layer 52 in built-in electric heating pipe 53, static heating cabinet 51 on set up enzyme feed liquor pipe 57.
According to the deer bone active peptide extraction device, a remote controller transmits a wireless signal which is received by a wireless receiving module and transmitted to a single chip microcomputer, the single chip microcomputer controls a motor I6, a linear motor I10, a linear motor II 21, a motor II 29, a motor III 42, a water pump, an electric heating pipe 53, an enzyme liquid inlet pipe 57, a PH liquid inlet pipe 46 and a PH test pen 47, and meanwhile the single chip microcomputer receives feedback information of the motor I6, the linear motor I10, the linear motor II 21, the motor II 29, the motor III 42, the water pump, the electric heating pipe 53, the enzyme liquid inlet pipe 57, the PH liquid inlet pipe 46 and the PH test pen 47.
The extraction method of the deer bone active peptide extraction device comprises the steps of firstly placing deer bones into a coarse stirring box 2 through a funnel 1, enabling a stirrer 5 driven by a motor I6 in the coarse stirring box 2 to start working, enabling a linear motor I10 to move leftwards after coarse stirring to drive an inclined rod I8 and a vertical rod 9 to move so that a sealing partition plate I4 moves downwards in a slide way 15, enabling the deer bones after coarse stirring to enter a grinding filter box 13 through a through pipe 12,
starting a motor II 29 in the grinding filter box 13 to drive a plurality of groups of grinders 32 to grind the deer bones, starting a linear motor II 21 to enable a sealing partition plate II 14 connected through a steel rope 19 to move leftwards, scraping the ground deer bones on the sealing partition plate II 14 downwards by a scraping plate I17 when the whole sealing partition plate II 14 passes through a trapezoidal through hole 16 to enable the deer bones to fall on a filter plate I34, filtering the deer bones by the filter plate I34, the filter plate II 35 and a filter plate III 36, and then falling into a mixing box 40 through a conical leakage groove 38 and an inclined through pipe 39,
add water through inlet tube 41 in mixing box 40, dilute, and start motor III 42 stirs, make water and the deer bone homogeneous mixing after grinding the filtration, it adds PH solution to advance pipe 46 through PH liquid, and detect through PH test pen 47, open left side sliding door 48 and right side sliding door 49 after the PH value is up to standard, the deer bone mixed liquid after making to add water gets into static heating box 51, add enzyme constant temperature hydrolysis in static heating box 51, boil the enzyme step that goes out after the hydrolysis is up to standard, after going out the enzyme, take off static heating box 51 from underframe 50 and pour the liquid in static heating box 51 into the centrifuge entirely, carry out the centrifugation, get the supernatant after the centrifugation.
Has the advantages that:
1. the sealing partition plate I is matched with the linear motor I to use, so that the coarsely stirred deer bones can automatically slide into the grinding filter box, the artificial pollution is reduced, and raw materials are not wasted.
2. The multiple grinders work together to increase the efficiency, and only one motor is needed to drive, so that the energy consumption is reduced.
3. The access door can be used for cleaning the filter plate I, the filter plate II and the filter plate III in a detachable mode, so that bacteria are prevented from breeding and sanitation is kept.
4. The stirring shaft and the stirring blades of the deer bone grinding and filtering device enable water and ground and filtered water to be mixed quickly to form deer bone mixed liquid, so that time is reduced, and working efficiency is improved.
5. The left side sliding door and the right side sliding door of the invention can lead the deer bone mixed liquid to directly enter the static heating box for enzyme-adding constant-temperature hydrolysis, and the static heating box is a sealed heat insulation box.
Description of the drawings:
FIG. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic structural diagram of the present invention.
Fig. 3 is a schematic diagram of the electrical signal connections of the present invention.
The specific implementation mode is as follows:
example 1
A deer bone active peptide extraction element, funnel 1 sets up in coarse stirring case 2, coarse stirring case 2's left side inner wall on set up pivot I3, pivot I3 on connect sealed baffle I4, sealed baffle I4's right-hand member slide on slide 15, sealed baffle I4 on set up agitator 5, agitator 5's central bottom insert motor I6, motor I6's right side set up sealed L shaped plate 7, motor I6's bottom surface left side set up down tube I8, down tube I8's bottom connect vertical pole 9, vertical pole 9 set up the fuselage top surface at linear electric motor I10, down tube 8 pass through opening I11, coarse stirring case 2's right side bottom set up and be obtuse siphunculus 12 with the level,
the siphunculus 12 connect and grind rose box 13, grind rose box 13 in set up sealed baffle II 14, the left side wall of grinding rose box 13 open and to have trapezoidal opening 16, the left side of sealed baffle II 14 pass trapezoidal opening 16, the right side of sealed baffle II 14 insert the right side wall of grinding rose box 13, the left side of grinding rose box 13 set up scraper blade I17, the right side of grinding rose box 13 set up scraper blade II 18, the left side outer end of sealed baffle II 14 connect steel cable 19, steel cable 19 connect linear electric motor II 21's fuselage top surface through solid fixed ring 20,
the top end of the grinding and filtering box 13 is connected with a ring-shaped frame 26 through an 11-shaped support 25, an annular through groove of the ring-shaped frame 26 penetrates through an n-shaped frame 27, the top end of the n-shaped frame 27 is connected with a motor II 29 through a coupler I28, the motor II 29 is connected with the ring-shaped frame 26 through a frame 30, the coupler I28 is also vertically connected with an extension rod 31, the bottom end of the extension rod 31 penetrates through the top surface of the grinding and filtering box 13 and is connected with a group of grinders 32, the bottom end of the n-shaped frame 27 is respectively connected with one grinder 32 through an inclined rod II 33, a filter plate I34 is arranged below the sealing partition plate II 14, a filter plate II 35 is arranged below the filter plate I34, a filter plate III 36 is arranged below the filter plate II 35, and an access door 37 is arranged on the right side surfaces of the filter plate I34, the filter plate II 35 and the;
the bottom end of the grinding and filtering box 13 is a combined leak groove which comprises a conical leak groove 38 and an inclined through pipe 39, and the inclined through pipe 39 is inserted into a mixing box 40.
Example 2
Embodiment 1 deer bone active peptide extraction element, mixing box 40 in set up inlet tube 41, inlet tube 41 connect the water pump, mixing box 40 left side set up motor III 42, motor III 42 connect (mixing) shaft 44 through shaft coupling II 43, the (mixing) shaft on set up stirring leaf 45, the right side of (mixing) shaft 44 set up PH liquid inlet tube 46 and PH test pen 47, the bottom of mixing box 40 set up left side pull door 48 and right side pull door 49, the place underframe 50 of left side pull door 48 and right side pull door 49 on slide static heating box 51, the bottom plate intermediate layer 52 of static heating box 51 in install electric heating pipe 53, static heating box 51 on set up enzyme feed liquor pipe 57.
Example 3
In the deer bone active peptide extraction device according to embodiment 1 or 2, a remote controller transmits a wireless signal to be received and transmitted to a single chip microcomputer by a wireless receiving module, the single chip microcomputer controls the motor i 6, the linear motor i 10, the linear motor ii 21, the motor ii 29, the motor iii 42, the water pump, the electric heating pipe 53, the enzyme liquid inlet pipe 57, the PH liquid inlet pipe 46 and the PH test pen 47, and the single chip microcomputer receives feedback information of the motor i 6, the linear motor i 10, the linear motor ii 21, the motor ii 29, the motor iii 42, the water pump, the electric heating pipe 53, the enzyme liquid inlet pipe 57, the PH liquid inlet pipe 46 and the PH test pen 47.
Example 4
The extraction method of the deer bone active peptide extraction device in embodiment 1-3, the deer bones are firstly placed into the rough mixing box 2 through the funnel 1, the mixer 5 driven by the motor I6 in the rough mixing box 2 starts to work, after rough mixing, the linear motor I10 moves leftwards to drive the inclined rod I8 and the vertical rod 9 to move, so that the sealing partition plate I4 moves downwards in the slideway 15, the deer bones after rough mixing enter the grinding filter box 13 through the through pipe 12,
starting a motor II 29 in the grinding filter box 13 to drive a plurality of groups of grinders 32 to grind the deer bones, starting a linear motor II 21 to enable a sealing partition plate II 14 connected through a steel rope 19 to move leftwards, scraping the ground deer bones on the sealing partition plate II 14 downwards by a scraping plate I17 when the whole sealing partition plate II 14 passes through a trapezoidal through hole 16 to enable the deer bones to fall on a filter plate I34, filtering the deer bones by the filter plate I34, the filter plate II 35 and a filter plate III 36, and then falling into a mixing box 40 through a conical leakage groove 38 and an inclined through pipe 39,
add water through inlet tube 41 in mixing box 40, dilute, and start motor III 42 stirs, make water and the deer bone homogeneous mixing after grinding the filtration, it adds PH solution to advance pipe 46 through PH liquid, and detect through PH test pen 47, open left side sliding door 48 and right side sliding door 49 after the PH value is up to standard, the deer bone mixed liquid after making to add water gets into static heating box 51, add enzyme constant temperature hydrolysis in static heating box 51, boil the enzyme step that goes out after the hydrolysis is up to standard, after going out the enzyme, take off static heating box 51 from underframe 50 and pour the liquid in static heating box 51 into the centrifuge entirely, carry out the centrifugation, get the supernatant after the centrifugation.
Example 5
The stirrer 5 of embodiment 1 comprises stirring blades 54, wherein the stirring blades 54 are radially arranged;
the grinder 32 comprises a grinding ring 55, the grinding ring 55 is sleeved on fixing posts 56, and three grinding rings 55 are arranged on each fixing post 56.
Example 6
The linear motor II 21 and the linear motor I10 in embodiment 1 slide on a linear motor slideway 22, the linear motor I10 slides on the top surface of the linear motor slideway 22, the linear motor II 21 slides on the bottom surface of the linear motor slideway 22, the left side of the linear motor slideway 22 is connected with a support column 23, and the right side of the linear motor slideway 22 is connected with an L-shaped connecting rod 24.
It is to be understood that the above description is not intended to limit the present invention, and the present invention is not limited to the above examples, and those skilled in the art may make modifications, alterations, additions or substitutions within the spirit and scope of the present invention.
Claims (2)
1. A deer bone active peptide extraction element, characterized by: funnel (1) sets up in coarse stirring case (2), the left side inner wall of coarse stirring case (2) on set up pivot I (3), pivot I (3) on connect sealed baffle I (4), the right-hand member of sealed baffle I (4) slide on slide (15), sealed baffle I (4) on set up agitator (5), the center bottom of agitator (5) insert motor I (6), the right side of motor I (6) set up sealed L shaped plate (7), the bottom surface left side of motor I (6) set up down tube I (8), the bottom of down tube I (8) connect vertical rod (9), vertical rod (9) set up the fuselage top surface at linear electric motor I (10), down tube I (8) pass through opening I (11), the right side bottom of coarse stirring case (2) set up and be obtuse siphunculus (12) with the level,
siphunculus (12) connect and grind rose box (13), grind rose box (13) in set up sealed bulkhead II (14), the left side wall of grinding rose box (13) open and to have trapezoidal opening (16), the left side of sealed bulkhead II (14) pass trapezoidal opening (16), the right side of sealed bulkhead II (14) insert the right side wall of grinding rose box (13), the left side of grinding rose box (13) set up scraper blade I (17), the right side of grinding rose box (13) set up scraper blade II (18), the left side outer end of sealed bulkhead II (14) connect steel cable (19), steel cable (19) through the fuselage top surface of solid fixed ring (20) connection linear electric motor II (21),
grind rose box (13) top connect annular frame (26) through "11" font support (25), the annular of annular frame (26) lead to the inslot and pass n shape frame (27), the top of n shape frame (27) pass through shaft coupling I (28) and connect motor II (29), motor II (29) connect annular frame (26) through frame (30), shaft coupling I (28) still connect extension rod (31) perpendicularly, the bottom of extension rod (31) pass the top surface of grinding rose box (13) and connect a set of mill (32), the bottom of n shape frame (27) connect a mill (32) through down tube II (33) respectively, the below of seal spacer II (14) set up filter plate I (34), the below of filter plate I (34) set up filter plate II (35), the below of filter plate II (35) set up filter plate (36), the right side surfaces of the filter plate I (34), the filter plate II (35) and the filter plate III (36) are provided with access doors (37);
the bottom end of the grinding filter box (13) is provided with a combined leakage groove, the combined leakage groove comprises a conical leakage groove (38) and an inclined through pipe (39), and the inclined through pipe (39) is inserted into a mixing box (40);
the device is characterized in that a water inlet pipe (41) is arranged in the mixing box (40), the water inlet pipe (41) is connected with a water pump, a motor III (42) is arranged on the left side of the mixing box (40), the motor III (42) is connected with a stirring shaft (44) through a coupler II (43), a stirring blade (45) is arranged on the stirring shaft, a pH liquid inlet pipe (46) and a pH test pen (47) are arranged on the right side of the stirring shaft (44), a left drawing door (48) and a right drawing door (49) are arranged at the bottom end of the mixing box (40), a bottom frame (50) where the left drawing door (48) and the right drawing door (49) are located slides on the static heating box (51), an electric heating pipe (53) is arranged in a bottom plate interlayer (52) of the static heating box (51), and an enzyme liquid inlet pipe (57) is arranged on the static heating box (51);
the remote controller transmits a wireless signal to be received and transmitted to the single chip microcomputer by the wireless receiving module, the single chip microcomputer controls the motor I (6), the linear motor I (10), the linear motor II (21), the motor II (29), the motor III (42), the water pump, the electric heating pipe (53), the enzyme liquid inlet pipe (57), the pH liquid inlet pipe (46) and the pH test pen (47), and meanwhile, the single chip microcomputer receives feedback information of the motor I (6), the linear motor I (10), the linear motor II (21), the motor II (29), the motor III (42), the water pump, the electric heating pipe (53), the enzyme liquid inlet pipe (57), the pH liquid inlet pipe (46) and the pH test pen (47).
2. The extraction method of deer bone active peptide extraction device according to claim 1, characterized in that: firstly, deer bones are placed into a coarse stirring box (2) through a funnel (1), a stirrer (5) driven by a motor I (6) in the coarse stirring box (2) starts to work, a linear motor I (10) moves leftwards after coarse stirring to drive an inclined rod I (8) and a vertical rod (9) to move so that a sealing partition plate I (4) moves downwards in a slide way (15), and the deer bones after coarse stirring enter a grinding filter box (13) through a through pipe (12),
starting a motor II (29) in the grinding filter box (13) to drive a plurality of groups of grinders (32) to grind the deer bones, starting a linear motor II (21) to enable a sealing partition plate II (14) connected through a steel rope (19) to move leftwards, scraping the ground deer bones on the sealing partition plate II (14) downwards by a scraper I (17) when the whole sealing partition plate II (14) passes through a trapezoidal through hole (16) to enable the deer bones to fall on a filter plate I (34), and after the filter plate I (34), the filter plate II (35) and a filter plate III (36) are filtered, the deer bones fall into a mixing box (40) through a conical leakage groove (38) and an inclined through pipe (39),
adding water into a mixing box (40) through a water inlet pipe (41), diluting, starting a motor III (42) for stirring, uniformly mixing the water with the ground and filtered deer bones, adding a pH solution through a pH solution inlet pipe (46), detecting through a pH test pen (47), opening a left drawing door (48) and a right drawing door (49) after the pH value reaches the standard, enabling the water-added deer bone mixed solution to enter a static heating box (51), adding enzyme into the static heating box (51) for constant-temperature hydrolysis, boiling and inactivating the enzyme after the hydrolysis reaches the standard, taking the static heating box (51) down from a bottom frame (50) after inactivation, pouring all liquid in the static heating box (51) into a centrifuge, centrifuging, and taking supernatant after centrifugation.
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| CN201810621584.2A CN108753886B (en) | 2018-06-15 | 2018-06-15 | Deer bone active peptide extraction device and extraction method |
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| CN201810621584.2A CN108753886B (en) | 2018-06-15 | 2018-06-15 | Deer bone active peptide extraction device and extraction method |
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| EP0566877A2 (en) * | 1992-04-09 | 1993-10-27 | Societe Des Produits Nestle S.A. | Enzymatic hydrolysis of proteines |
| CN104561210A (en) * | 2015-01-27 | 2015-04-29 | 张恒 | Extraction method of deer bone peptide |
| CN107034139A (en) * | 2017-04-26 | 2017-08-11 | 黑龙江省野生动物研究所 | A kind of deer whip active peptide extraction element and extracting method |
| CN107365350A (en) * | 2017-08-30 | 2017-11-21 | 郑州大学 | A kind of extraction element of high efficiency extraction Moringa leaf protein |
| CN107897943A (en) * | 2017-11-28 | 2018-04-13 | 苏洁 | A kind of method that deer bone native peptides are extracted in the bone from deer |
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2018
- 2018-06-15 CN CN201810621584.2A patent/CN108753886B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0566877A2 (en) * | 1992-04-09 | 1993-10-27 | Societe Des Produits Nestle S.A. | Enzymatic hydrolysis of proteines |
| CN104561210A (en) * | 2015-01-27 | 2015-04-29 | 张恒 | Extraction method of deer bone peptide |
| CN107034139A (en) * | 2017-04-26 | 2017-08-11 | 黑龙江省野生动物研究所 | A kind of deer whip active peptide extraction element and extracting method |
| CN107365350A (en) * | 2017-08-30 | 2017-11-21 | 郑州大学 | A kind of extraction element of high efficiency extraction Moringa leaf protein |
| CN107897943A (en) * | 2017-11-28 | 2018-04-13 | 苏洁 | A kind of method that deer bone native peptides are extracted in the bone from deer |
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