CN108753660A - One plant of Methylobacterium and its application - Google Patents
One plant of Methylobacterium and its application Download PDFInfo
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- CN108753660A CN108753660A CN201810646127.9A CN201810646127A CN108753660A CN 108753660 A CN108753660 A CN 108753660A CN 201810646127 A CN201810646127 A CN 201810646127A CN 108753660 A CN108753660 A CN 108753660A
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- methylobacterium
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention belongs to microorganisms technical fields, and in particular to one plant of Methylobacterium and its application.One plant of Methylobacterium, Classification And Nomenclature is torsional demethylation coli (Methylobacterium extorquens), it is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.15777, and the deposit date is on Mays 21st, 2018.Application of the above-mentioned Methylobacterium in preparing microbial manure.Further, it adds the Methylobacterium and microorganism seed coating agent is made.One plant of Methylobacterium disclosed by the invention and its application have the advantages that：1, the chemical fertilizer and Pesticide use during corn planting are reduced, and then realizes high yield；2, it more plays an important role to the seed for being easy devitalization.
Description
Technical field
The invention belongs to microorganisms technical fields, and in particular to one plant of Methylobacterium and its application.
Background technology
Corn is second-biggest-in-the-world crop, and China is second-biggest-in-the-world maize production state, produces more than 6,750 ten thousand tons of corn per year.It is beautiful
Rice is that China has the call and one of extensive staple food crop of application field.
In agricultural production, a large amount of applications of chemical fertilizer and pesticide cause soil degradation, crop and yield decline,
The problems such as deterioration of the ecological environment, threatens China's food and Environmental security.Therefore using the physiological property of microorganism, production is provided and is made
Object grows required nutrient, either improves the graininess of soil or resists the pest and disease damage in environment, in safe and high quality agricultural production
Product are produced to be continuously improved with the status in ecological environmental protection.
One carbon organic compound (One-carbon organic compounds) is widely present in nature, methyl battalion
Bacteria (Methylotrophy), which is one kind, can utilize the micro- of non-C-C keys low carbon compound (such as methane, methanol, formaldehydes)
Biology.Since this kind of bacterium is mostly rod-shaped, also referred to as Methylobacterium (Methylotrophic bacteria).Methyl bar
The most of pinkiness of bacterium of Pseudomonas, they are commonly referred to as PPFM bacteriums (pink-pigmented facultatively
methylotrophic bacteria).PPFM bacteriums usually colonize in plant roots, stem, on leaf table, especially the abundantest with leaf table,
PPFM, for carbon source, and generates plant hormone such as auxin (IAA), cell division with the one-carbon compound (such as methanol) of plant secretion
Plain (CK), and have positive acting to plant nitrogen metabolism, germination, photosynthesis, plant nutrient etc..
Most crops are sprouted in seed and early stage plantlet stage is most sensitive to environment-stress.The plant piece-root grafting just germinateed
Touch fertilizer speed, for plant intake fertilizer present in nutrients ability and plant first three weeks initiation and
The successful of growth is of great significance.Using seed coating technology can make various adding ingredients directly, be evenly distributed in kind
Around sub, without being largely dispersed in seedbed so that the usage amount of fertilizer and agrochemical product is greatly decreased, reduce the means of agricultural production at
This consumption, and seed can be made more to be protected during storage, it is not easy to devitalization, especially to being easy funeral
The seed of inactivation power more plays an important role.
Coating agent for seed is most absolutely at present uses chemicals, and chemical residual can be caused by using, pollution suddenly and
Water source, and biological seed coating agent is very rare.
Invention content
Goal of the invention：The present invention has made improvements in view of the above-mentioned problems of the prior art, i.e. first mesh of the invention
Be one plant of Methylobacterium is disclosed.Second object of the present invention is to disclose the application of one plant of Methylobacterium.
Technical solution：One plant of Methylobacterium, Classification And Nomenclature are torsional demethylation coli (Methylobacterium
Extorquens), it is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number CGMCC
No.15777, the deposit date is on Mays 21st, 2018.
Application of the above-mentioned Methylobacterium in preparing microbial manure.
Further, it adds the Methylobacterium and microorganism seed coating agent is made.
Further, the preparation method of the microorganism seed coating agent is：
By Methylobacterium, water, Diacloden three be uniformly mixed to get.
Further, in the microorganism seed coating agent viable bacteria quantity >=106cfu/mL。
Further, Methylobacterium, water, Diacloden mass ratio be (20~25)：(10~20)：(40~60).
Further, Methylobacterium, water, Diacloden mass ratio be 25:20:55.
Advantageous effect：One plant of Methylobacterium disclosed by the invention and its application have the advantages that：
It provides for improveing corn seed to the microorganism seed coating agent for promoting seedling growth during seedling growth, it should
Microorganism seed coating agent, by less toxic, efficient, environmental-friendly feature, is reduced beautiful by adding torsional demethylation coli ingredient
Chemical fertilizer and Pesticide use in rice planting process, and then realize high yield；
Using microorganism seed packaging technique can make various adding ingredients directly, efficiently and be evenly distributed in seed week
Enclose, without be dispersed in seedbed, and seed can be made more to be protected during storage, it is not easy to lose and live
Power especially more plays an important role to the seed for being easy devitalization.
Description of the drawings
Fig. 1 is the tablet culture figure of the Methylobacterium of the present invention.
Fig. 2 is the thalli morphology figure of the Methylobacterium of the present invention.
Fig. 3 is the phyletic evolution tree graph of the Methylobacterium of the present invention
Fig. 4 is Maize at Seedling Stage 20d, experimental group (right side) and control group plant (left side), blank seed coat agent be coated (in) life
Long comparison diagram.
Specific implementation mode：
The specific implementation mode of the present invention is described in detail below.
One, the separation and screening of Methylobacterium bacterial strain
Sample collected pachyrhizus blade from the farmland ground in the Guangxi province Chongzuo City Daxin County townshiies Na Ling.
1g pachyrhizus blades are weighed, are added in the tween water of sterilized 100mL a concentration of 0.1%, it is broken to hold hand held
Machine smashes blade, and leaf liquid gradient is taken to be diluted to 10-2、10-3Then concentration is coated on AMS solid inorganic salt culture mediums
In, pink single bacterium colony is chosen after culture 5d, 5d are inverted in 28 DEG C of constant incubators, continues to be inoculated in the training of AMS solid inorganic salts
It supports on base, culture 5d is inverted in 28 DEG C of constant incubators, then observes bacterium colony kenel, light microscope is used in combination to observe thalline type
State (1000X).
The formula of AMS solid inorganic salt culture mediums is：Anhydrous potassium dihydrogen phosphate 700mg, anhydrous potassium dihydrogenphosphate 540mg,
Bitter salt 1g, anhydrous ammonium chloride 500mg, chloride dehydrate calcium 200mg, iron sulfate heptahydrate 4mg, Zinc vitriol
100 μ g, four chloride hydrate manganese, 30 μ g, 300 μ g of anhydrous boric acid, 200 μ g of cobalt chloride hexahydrate, 10 μ g of chloride dehydrate copper, six hydrations
The first after 0.22 μm of membrane filtration is added after high-temperature sterilization in 20 μ g of nickel chloride, dehydration sodium molybdate 60 μ g, agar 15g, moisturizing to 1L
Alcohol 5mL.
Bacterium colony kenel is as shown in Figure 1.The bacterium colony of the bacterial strain is in opaque pink, and shape is circle, upward, side
Edge is neat, dry.
Thalline kenel is as shown in Figure 2.The thalline of the bacterial strain is without gemma, atrichia, oval.
Bacterial strain Physiology and biochemistry identification according to《Common bacteria system identification handbook》It carries out.Identified, which is methyl
Bacillus, main physiological activity G- contact enzyme positive, cannot hydrolyze starch.
Then, to the sequence of the bacterial strain carried out 16S sequence fragments (amplimer Yu Measuring sequence primers be 27F：5'-
AGAGTTTGATCCTGGCTCAG-3' and 1492R：It 5'-GGTTACCTTGTTACGACTT-3') measures, measurement result is shown in sequence
Table SEQ ID NO.1；Sequence 16SrRNA sequence homology analysis, Phylogenetic Analysis, by the homologous comparisons of BLAST, we are really
The nearest kind of the fixed bacterial strain is Methylobacter.And by methyl functioning gene mxaF genes (methanol
Dehydrogenase alpha subunit) (amplimer Yu Measuring sequence primers are segment：mxaF-f(f1003)5'-
GCGGCACCAACTGGGGCTGGT-3' and mxaF-r (r1561) 5'-GGGCAGCATGAAGGGCTCCC-3') it measures, measure knot
Fruit sees sequence table SEQ ID NO.2.It is to turn round demethylation to obtain this Methylobacterium through maxF sequence homology analysis, Phylogenetic Analysis
Bacillus (Methylobacterium extorquens).
The sequence of Methylobacter is obtained from GenBank databases, and combines the mode standard bacterium of ncbi database
Sequence has carried out clustering and system using MEGA5.0 softwares using adjacent method (Neighbor-Joining method)
Chadogram builds, and the systematic evolution tree of structure is shown in Fig. 3.
So far judge the bacterial strain for torsional demethylation coli (Methylobacterium extorquens)；Bacterial strain is denoted as first
Base shaft bacterium MN29385.Then, Methylobacterium MN29385 is sent to China Committee for Culture Collection of Microorganisms's commonly micro- life
Object center (CGMCC) carries out preservation, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date：2018 5
The moon 21, deposit number：CGMCC No.15777.
Two, microbial manure is prepared
Microorganism seed coating agent is made in Methylobacterium MN29385 by us.
The preparation process of the microorganism seed coating agent is specific as follows：
1, the original seed of Methylobacterium MN29385 is connected on culture dish and is activated, is then inoculated in spare on test tube slant；
2, the strain in test tube is inoculated in the 250mL shaking flasks equipped with 100mL AGPS culture mediums, 28 DEG C of isothermal vibrations
It cultivates to logarithmic phase；
3, the concentration that bacterium solution in shaking flask is measured using light splitting luminance meter, when the concentration of bacterium solution reaches 108When cfu/mL, according to
The mass ratio mixing of water 10~20%, the bacterium solution 20~25% of Jia Ji Rod bacterium, Diacloden 40~60%, preferably water 20%, first
Then the bacterium solution 25% of Ji Rod bacterium, Diacloden 55% are vibrated to substance dissolving with shaker and are coated to get to microorganism seed
Agent, after testing, the quantity of viable bacteria is more than 10 in the microorganism seed coating agent6cfu/mL。
The formula of AGPS culture mediums is：Anhydrous potassium dihydrogen phosphate 700mg, anhydrous potassium dihydrogenphosphate 540mg, seven hydrated sulfuric acids
Magnesium 1g, anhydrous ammonium chloride 500mg, chloride dehydrate calcium 200mg, iron sulfate heptahydrate 4mg, 100 μ g of Zinc vitriol, four hydrations
30 μ g of manganese chloride, it 300 μ g of anhydrous boric acid, 200 μ g of cobalt chloride hexahydrate, 10 μ g of chloride dehydrate copper, 20 μ g of Nickel dichloride hexahydrate, takes off
60 μ g of water sodium molybdate, glycerine 10g, peptone 10g, moisturizing to 1L, high-temperature sterilization.
Three, corn planting experiment is carried out
Corn seed uses：Zheng Dan 958.
Soil：Carbon soil is with perlite according to 2：1 volume ratio mixes, and is used after sterilizing.
The much the same corn seed of size is selected, by obtained microorganism seed coating agent prepared above with 1:50~1:80
Weight ratio and corn seed mix, make bacterial strain uniform adsorption on corn seed, dry, obtain Maize Seeds Coated, wait for
With.
It takes 10 Maize Seeds Coateds in the centrifuge tube equipped with 10ml sterile waters, vibrates 2~3min, make on corn seed
Bacterial strain fall off in sterile water, be then diluted to 10 again-2、10-3, dilution is coated with to counting on AMS tablets, is calculated every
The coating bacterium number of seed.
Experimental group：By the plantation of Methylobacterium seed coat agent Maize Seeds Coated in the small plastic tub of 10cm diameters, per basin three
Seed, totally 30 plants, greenhouse experiments repeat three times, are arranged using random district's groups, and being averaged for plant is measured in Maize at Seedling Stage 20d
Plant height, average stem length, stem thickness, mean fresh.
Blank seed coat agent is coated：The corn seed of 35% Diacloden seed coat agent coating is planted in the small plastics of 10cm diameters
In basin, per three seeds of basin, totally 30 plants, greenhouse experiments repeat three times, are arranged using random district's groups, are surveyed in Maize at Seedling Stage 20d
The average plant height of field planting strain, average stem length, stem thickness, mean fresh.
Control group：Uncoated corn seed is planted in the small plastic tub of 10cm diameters, per three seeds of basin, totally 30
Strain, greenhouse experiments repeat three times, are arranged using random district's groups, and the average plant height of plant, average stem are measured in Maize at Seedling Stage 20d
Length, stem thickness, mean fresh.
Four, test result
Maize at Seedling Stage 20d, experimental group (MN29385), the coating of blank seed coat agent and the growing state of control group plant are shown in
Fig. 4.
Maize at Seedling Stage 20d, experimental group (MN29385), the average plant height of blank seed coat agent coating and control group plant
(cm), average stem length (cm), stem thickness (cm), mean fresh (g) are specific as follows：
| Processing | Plant height (cm) | Stem length (cm) | Stem thickness (cm) | Fresh weight (g) |
| Control group | 33.75±2.41 | 9.47±041 | 2.9±0.18 | 2.43±0.21 |
| Blank seed coat agent is coated | 33.16±2.84 | 9.78±0.38 | 3.01±0.12 | 2.41±0.19 |
| Experimental group | 37.42±2.67 | 10.52±0.63 | 3.33±0.16 | 2.96±0.25 |
As seen from the above table, corn seed is after the coating of the microorganism seed coating agent of the present invention, either plant height, stem
Long, stem thickness or fresh weight are all substantially better than control group.
Five, conclusion
The Methylobacterium MN29385 of the present invention can be obviously promoted plant strain growth, reinforce seedling stage health, have apparent volume increase
Potentiality, and can be combined with insect control seed coat agent on the market, there is height application elasticity in microorganism seed packaging technique field
With value.
Embodiments of the present invention are elaborated above.But present invention is not limited to the embodiments described above,
Technical field those of ordinary skill within the scope of knowledge, can also do without departing from the purpose of the present invention
Go out various change.
Sequence table
<110>Guangzhou bacterium colony bio tech ltd
<120>One plant of Methylobacterium and its application
<130> 1
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1299
<212> DNA
<213>Torsional demethylation coli (Methylobacterium extorquens)
<400> 1
gtcagtggca gacgggtgag taacacgtgg gaacgtaccc ttcggttcgg aataactcag 60
ggaaacttga gctaataccg gatacgccct tttggggaaa ggtttactgc cgaaggatcg 120
gcccgcgtct gattagcttg ttggtggggt aacggcctac caaggcgacg atcagtagct 180
ggtctgagag gatgatcagc cacactggga ctgagacacg gcccagactc ctacgggagg 240
cagcagtggg gaatattgga caatgggcgc aagcctgatc cagccatgcc gcgtgagtga 300
tgaaggcctt agggttgtaa agctcttttg tccgggacga taatgacggt accggaagaa 360
taagccccgg ctaacttcgt gccagcagcc gcggtaatac gaagggggct agcgttgctc 420
ggaatcactg ggcgtaaagg gcgcgtaggc ggccgattaa gtcgggggtg aaagcctgtg 480
gctcaaccac agaattgcct tcgatactgg ttggcttgag accggaagag gacagcggaa 540
ctgcgagtgt agaggtgaaa ttcgtagata ttcgcaagaa caccagtggc gaaggcggct 600
gtctggtccg gttctgacgc tgaggcgcga aagcgtgggg agcaaacagg attagatacc 660
ctggtagtcc acgccgtaaa cgatgaatgc cagccgttgg cctgcttgca ggtcagtggc 720
gccgctaacg cattaagcat tccgcctggg gagtacggtc gcaagattaa aactcaaagg 780
aattgacggg ggcccgcaca agcggtggag catgtggttt aattcgaagc aacgcgcaga 840
accttaccat cccttgacat ggcatgttac ctcgagagat cggggatcct cttcggaggc 900
gtgcacacag gtgctgcatg gctgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc 960
cgcaacgagc gcaacccacg tccttagttg ccatcattca gttgggcact ctagggagac 1020
tgccggtgat aagccgcgag gaaggtgtgg atgacgtcaa gtcctcatgg cccttacggg 1080
atgggctaca cacgtgctac aatggcggtg acagtgggac gcgaagccgc gaggtggagc 1140
aaatccccaa aaaccgtctc agttcggatt gcactctgca actcgggtgc atgaaggcgg 1200
aatcgctagt aatcgtggat cagcacgcca cggtgaatac gttcccgggc cttgtacaca 1260
ccgcccgtca caccatggga gttggtctta cccgacggc 1299
<210> 2
<211> 454
<212> DNA
<213>Torsional demethylation coli (Methylobacterium extorquens)
<400> 2
tcacgcttcg gatcgtacga gtcgtgaccc tggttgtggt aacccatcgc cgaggggcag 60
atgtccttgg cgaggtggtc catccgggtg ccgtattcgg gatcgcgcac cggctggccc 120
gtcttgagat ccaccgactt gaacacgttg accgtgtcgt cgagcttgtt cgccgagacg 180
agcgcgccgt cggtccggtc gagcgtgtag acgatgccgt tgcggtccgg gtgggtcagc 240
agcttgcggg ccttgccgtc cttgtccttc tgctcggaga gcatcatgac gttgacgccg 300
gcatagtccc actcgtcgtg cggggtcttc tggtagccga acttggcttc acccgtatcg 360
gcatcgcggc cgaagatcgt catcgtccac ttgttgtcgc ccggacgcat ggtctcgttc 420
cacggcgccg ggttgccggt gccgaagtag atca 454
Claims (7)
1. one plant of Methylobacterium, Classification And Nomenclature is torsional demethylation coli (Methylobacterium extorquens), is deposited in
China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number are CGMCC No.15777, preservation date
For on May 21st, 2018.
2. application of the Methylobacterium according to claim 1 in preparing microbial manure.
3. application according to claim 2, which is characterized in that add the Methylobacterium and microorganism seed coating agent is made.
4. application according to claim 3, which is characterized in that the preparation method of the microorganism seed coating agent is：
By Methylobacterium, water, Diacloden three be uniformly mixed to get.
5. application according to claim 4, which is characterized in that the quantity of viable bacteria in the microorganism seed coating agent >=
106cfu/mL。
6. application according to claim 4, which is characterized in that Methylobacterium, water, Diacloden mass ratio be (20~
25)：(10~20)：(40~60).
7. application according to claim 6, which is characterized in that Methylobacterium, water, Diacloden mass ratio be 25:20:
55。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810646127.9A CN108753660A (en) | 2018-06-21 | 2018-06-21 | One plant of Methylobacterium and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810646127.9A CN108753660A (en) | 2018-06-21 | 2018-06-21 | One plant of Methylobacterium and its application |
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| Publication Number | Publication Date |
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| CN108753660A true CN108753660A (en) | 2018-11-06 |
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ID=63976163
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| CN201810646127.9A Pending CN108753660A (en) | 2018-06-21 | 2018-06-21 | One plant of Methylobacterium and its application |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109868228A (en) * | 2019-03-28 | 2019-06-11 | 慕恩（广州）生物科技有限公司 | One plant of nearly rose color lock shadow yeast and its application |
| CN110684669A (en) * | 2019-03-07 | 2020-01-14 | 慕恩（广州）生物科技有限公司 | Penicillium capsulatum and application thereof |
| CN111778197A (en) * | 2020-08-06 | 2020-10-16 | 广东省生物工程研究所（广州甘蔗糖业研究所） | Methylobacterium strain with high growth promoting efficiency and application thereof |
| CN112195132A (en) * | 2020-10-26 | 2021-01-08 | 中国科学院上海高等研究院 | Methylobacterium thermophilum and application thereof in organic solid waste |
| CN114606278A (en) * | 2022-02-15 | 2022-06-10 | 浙江大学杭州国际科创中心 | Method for catalytically synthesizing 2,5-furandicarboxylic acid by using methylobacterium |
| CN116179457A (en) * | 2022-12-12 | 2023-05-30 | 青岛农业大学 | Method for promoting plant probiotics methylobacterium She Jiding to be bred |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110684669A (en) * | 2019-03-07 | 2020-01-14 | 慕恩（广州）生物科技有限公司 | Penicillium capsulatum and application thereof |
| CN110684669B (en) * | 2019-03-07 | 2023-05-30 | 慕恩（广州）生物科技有限公司 | Penicillium shell and application thereof |
| CN109868228A (en) * | 2019-03-28 | 2019-06-11 | 慕恩（广州）生物科技有限公司 | One plant of nearly rose color lock shadow yeast and its application |
| CN109868228B (en) * | 2019-03-28 | 2020-08-11 | 慕恩（广州）生物科技有限公司 | Sporobolomyces fumosoroseus and application thereof |
| CN111778197A (en) * | 2020-08-06 | 2020-10-16 | 广东省生物工程研究所（广州甘蔗糖业研究所） | Methylobacterium strain with high growth promoting efficiency and application thereof |
| CN112195132A (en) * | 2020-10-26 | 2021-01-08 | 中国科学院上海高等研究院 | Methylobacterium thermophilum and application thereof in organic solid waste |
| CN112195132B (en) * | 2020-10-26 | 2022-07-05 | 中国科学院上海高等研究院 | Methylobacterium thermophilum and application thereof in organic solid waste |
| CN114606278A (en) * | 2022-02-15 | 2022-06-10 | 浙江大学杭州国际科创中心 | Method for catalytically synthesizing 2,5-furandicarboxylic acid by using methylobacterium |
| CN114606278B (en) * | 2022-02-15 | 2023-07-11 | 浙江大学杭州国际科创中心 | Method for synthesizing 2,5-furandicarboxylic acid by using methyl bacillus catalysis |
| CN116179457A (en) * | 2022-12-12 | 2023-05-30 | 青岛农业大学 | Method for promoting plant probiotics methylobacterium She Jiding to be bred |
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Application publication date: 20181106 |