CN108753629A - One plant of Rhizopus oryzae and its application with salt resistance ability - Google Patents
One plant of Rhizopus oryzae and its application with salt resistance ability Download PDFInfo
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- CN108753629A CN108753629A CN201810748022.4A CN201810748022A CN108753629A CN 108753629 A CN108753629 A CN 108753629A CN 201810748022 A CN201810748022 A CN 201810748022A CN 108753629 A CN108753629 A CN 108753629A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/845—Rhizopus
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention discloses a Rhizopus oryzae (Rhizopus oryzae), which is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on January 22nd, 2018, and biological deposits number are：CGMCC NO.15267.The present invention screens isolated Rhizopus oryzae (Rhizopus oryzae) good salt tolerance, survival ability is strong especially under high salt concentration environment under severe conditions, the speed of growth is fast, in plant rhizosphere soil can rapid, high volume colonize, can be used for the soil improvement in salt-soda soil.Salt damage symptom can be significantly slowed under salt stress, increased the biomass of plant to promote growth, helped to improve the salt tolerance of plant.
Description
Technical field
The present invention relates to agriculture microorganism and ecological restoration technical fields, and in particular to one plant of rice root with salt resistance ability
Mould and its application.
Background technology
The salinization of soil is one of the main abiotic stress factor of the production of current threat Global Agriculture and crop yield,
Irrigating land for the whole world nearly 50% is all threatened by different degrees of salt stress at present, China about 9.9 × 107hm2Soil
There are problems that salt marsh harm, and compromised area is just increasing year by year, and local area ecological is caused to deteriorate.Ecological Effect is being advocated now
Under the premise of benefit is attached most importance to, biological modification measure has become the hot spot of research.
Current main biological modification measure has cultivation and plants the crop of saline-alkali tolerant and grinding for salt tolerant genetically modified plants
Study carefully, but often the period is long, costly for such method, and also plant transgenic method does not also accept extensively approval by society.
And microorganism is used to carry out a kind of new approaches that improvement belongs to salt-soda soil biological modification measure to salt-soda soil.How much microorganism is ground
Power or an index in salt-soda soil, but salt-soda soil micro organism quantity is few, and main cause is to be unfavorable for microorganism under saline-alkali environment
Existence, high salt concentration ion pair microbial cell cause dehydration and generate inhibiting effect to metabolism, this not only inhibits microorganism
Growth, and reduce Effect of Ecological Restoration of the microorganism to salt-soda soil.Strain is given birth to salt-soda soil using microorganism
The basis of object improvement, the bottleneck for limiting the development of salt-soda soil biological modification at present is mainly the selection and breeding problem of high-efficiency strain, due to salt
Alkali Soil Microorganism flora number of species it is relatively fewer, it is desirable to isolate purpose strain and be extremely not easily accomplished, especially
It isolates and is resistant to bacterial strain with high salt.
Rhizopus oryzae (Rhizopus oryzae) is a kind of common mould, is widely distributed in nature.Rhizopus oryzae
It plays highly important role in brewing, since it can generate highly active amylase, is commonly used in brewing industry
The saccharolytic of starchy material.Rhizopus oryzae can also generate malic acid, γ-aminobutyric acid, lactic acid, lemon in growth course
A variety of organic acids such as acid, fumaric acid, are widely used in the fields such as food, medicine, feed.But at present also not about Rhizopus oryzae
The report of salt tolerance.
Invention content
For the above-mentioned prior art, the object of the present invention is to provide one plant of Rhizopus oryzaes with salt resistance ability.The Rhizopus oryzae
It is resistant to salt stress caused by 10% or less salinity and pH9.5 alkaline stresses below, can effectively carry out alkaline land soil
Improvement.
Specifically, the present invention relates to following technical schemes：
Bacterial strain provided by the invention with salt resistance ability is Rhizopus oryzae (Rhizopus oryzae), the bacterial strain in
On January 22nd, 2018 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (abbreviation CGMCC, address
For：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3), biological deposits number are：CGMCC NO.15267.
The Rhizopus oryzae (Rhizopus oryzae) has following feature：
Bacterium colony is dense, initially it is white, after become dark brown.Rhizoid is flourishing, and branch is in root shape.Sporangiophore is upright, with vacation
Root is brown to life.Capsule axis is spherical in shape, oval, in filbert.Sporangium is spherical in shape, is in black after old.Sporangiospore is in ellipse
It is round, spherical, have no zygosperm.
Metabolin containing the Rhizopus oryzae (Rhizopus oryzae) or/and Rhizopus oryzae (Rhizopus oryzae)
Microbial inoculum also belongs to protection scope of the present invention.
The microbial inoculum removes the metabolism comprising Rhizopus oryzae (Rhizopus oryzae) or/and Rhizopus oryzae (Rhizopus oryzae)
Outside object, it may also include auxiliary material, such as turf, the excrement of animal, the stalk of all kinds of crops, straw, peanut skin.
In the microbial inoculum, the metabolism of Rhizopus oryzae (Rhizopus oryzae) or/and Rhizopus oryzae (Rhizopus oryzae)
The shape of the zymotic fluid of living cells, living cells that object can be cultured, the filtrate of cell culture or cell and the mixture of filtrate
Formula exists.
The dosage form of the microbial inoculum can be liquor, emulsion, suspending agent, granule, pulvis, wettable powder or water-dispersed
Agent.
Following 1) -3 of Rhizopus oryzae (Rhizopus oryzae)) in any application also belong to protection scope of the present invention；
1) application in alkaline land soil reparation；
2) application for promoting plant to be grown under salt stress environment；
3) application in preparing the biological organic fertilizer for alkaline land soil reparation.
Above-mentioned microbial inoculum is above-mentioned 1) -3) in any application also belong to protection scope of the present invention.
The present invention also provides a kind of preparation for alkaline land soil reparation, active constituent is Rhizopus oryzae (Rhizopus
Oryzae), zymotic fluid, bacteria suspension and/or the supernatant of Rhizopus oryzae (Rhizopus oryzae).
The present invention further provides a kind of method of alkaline land soil reparation, by Rhizopus oryzae (Rhizopus oryzae)
Zymotic fluid, cellular lysate liquid or spore suspension be sprayed in alkaline land soil；Or by the Rhizopus oryzae (Rhizopus
Oryzae it) is inoculated into wood sawdust and cultivates, adjust pH to 7.0, cultivated 7 days at 30 DEG C, then will be inoculated with Rhizopus oryzae
The wood sawdust of (Rhizopus oryzae) applies in alkaline land soil.
Beneficial effects of the present invention：
(1) present invention screens isolated Rhizopus oryzae (Rhizopus oryzae) good salt tolerance, under severe conditions especially
It is that survival ability is strong under high salt concentration environment, and the speed of growth is fast, in plant rhizosphere soil can rapid, high volume colonize, can use
Soil improvement in salt-soda soil.
(2) present invention, which screens isolated Rhizopus oryzae (Rhizopus oryzae), to significantly slow salt under salt stress
Evil symptom, increases the biomass of plant to promote growth, helps to improve the salt tolerance of plant, improves soil texture.
Description of the drawings
Fig. 1：Soil agreegate composition measuring result；
Fig. 2：Each group clover chlorophyll content measurement result；
Fig. 3：Each group alfalfa growing elevation measurement result；
Fig. 4：Each group clover catalase activity measurement result；
Fig. 5：Each group clover glutathione content measurement result.
Specific implementation mode
It is noted that following detailed description is all illustrative, it is intended to provide further instruction to the application.Unless another
It indicates, all technical and scientific terms used herein has usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
As background technology part is introduced, the bottleneck for limiting the development of salt-soda soil biological modification at present is mainly Black Liquor with Efficient Bacteria
The selection and breeding problem of kind, since microorganism species number of species are relatively fewer in alkaline land soil, it is desirable to isolate purpose strain pole
To be not easily accomplished, especially isolates and be resistant to bacterial strain with high salt.Present invention separation screening from Alfalfa Rhizospheric Soils obtains
There is Efficient salt-tolerant alkali ability (being resistant to salt stress caused by 10% or less salinity and pH8.5 alkaline stresses below) to one plant
Rhizopus oryzae, the Rhizopus oryzae in improvement soil, increase soil fertility, have broad application prospects in alkaline land soil reparation,
Thus propose the present invention.
In order to enable those skilled in the art can clearly understand the technical solution of the application, below with reference to tool
The technical solution of the application is described in detail in the embodiment of body.If the experiment actual conditions being not specified in embodiment, usually according to
Normal condition, or the condition recommended according to Reagent Company；Reagent as used in the following examples, consumptive material etc., such as without special
Illustrate, can be obtained by commercial sources.
Embodiment 1：The separation and identification of bacterial strain
1. the separation of bacterial strain：
(1) primary dcreening operation：
1g soil samples (picking up from Dongying city Hekou District Alfalfa Rhizospheric Soils) are weighed in 100ml sterile waters, are placed in 30
150rpm vibrates 10min in DEG C shaking table, takes 100 μ l 10-2Dilution is coated on the PDA culture medium tablet that salinity is 2%,
Culture to bacterium colony is grown at 30 DEG C.
(2) secondary screening：
The obtained bacterium colony of primary dcreening operation is inoculated by plate streak separation in the higher culture medium of salinity, concentration is according to ladder
Degree improves.It eugonic bacterial strain will be isolated and purified by method of scoring under maximum concentration.
Wherein, the formula of PDA culture medium：Peeled potatoes 200g, glucose 20g, agar 14g, distilled water 1000ml.
Through screening operation, the bacterial strain of one plant of salt tolerant, number SDZhuGeYP001 are obtained under 10% salinity.
2. the identification of bacterial strain：
(1) Morphological Identification：
Bacterium colony is dense, initially it is white, after become dark brown.Rhizoid is flourishing, and branch is in root shape.Sporangiophore is upright, with vacation
Root is brown to life.Capsule axis is spherical in shape, oval, in filbert.Sporangium is spherical in shape, is in black after old.Sporangiospore is in ellipse
It is round, spherical, have no zygosperm.
(2) Molecular Identification：
Aseptically fungal DNA extraction kits is utilized to extract genomic DNA, after extracting product utilization PCR amplification
It is detected into row agarose gel electrophoresis, jel product cutting recycles and utilizes fungi ITS sequence to be sequenced after purification, sequence such as SEQ
It is specific as follows shown in ID NO.1：
TCCCCCTGACTTCGATCATAGTTTGAAAGTTACTGGATTATACTCTTGTACTTTACTTCCTGGGCGAACCAAAAAAA
AAGATCCTGAGACCAGCGTAATATTCCTGCCTAGCAAGCCAGACAGAAAATCACACACATTTTAGGTGCTCACTGTA
ATAAAACAGCGATGCGACCCATCACCACATAAACAAATGTTATGTGTGGGTTTGTGATGATACTGAAGCAGGCGTAC
TCTATAGAAAAACCATAGAGTGCAAGCTGCGTTCAAAGACTCGATGATTCACTGAATATGCAATTCACACTAGTTAT
CGCACTTTGCTACGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTGTTTTTTATTAAACTTTATA
ATACTGAATTTCTAGGTTTATTATGAAGGGTACTCCTGAAACCAGGAGTGGCATCGATCAAACCCCAGATAGGTCTA
CCCATGACCAGTCTGAGTCTCTCAGCCAAATTTTCACAGTGTAGAAGCAATCACTTACCCCAGAGGAAACCCTAAGG
TAAGGCGCTTTAACATAATTAATGATCCTTCCGCAGGTTCACCTACGGAAG。
Sequencing result is committed to Gene Bank and carries out sequence alignment using BLAST, and fungi is determined using sequence similarity
Race relation.
Based on the above-mentioned Morphological Identification to bacterial strain and molecules qualification result, isolated bacterial strain is accredited as a meter root
Mould (Rhizopus oryzae).And biological deposits are carried out to the bacterial strain, preservation information is as follows：
Strain name：Rhizopus oryzae
Latin name：Rhizopus oryzae
Preservation mechanism：China Committee for Culture Collection of Microorganisms's common micro-organisms center
Preservation mechanism is referred to as：CGMCC
Address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Preservation date：2018.01.22
Collection is registered on the books number：CGMCC NO.15267.
Embodiment 2：Pot experiment
1. the preparation of microbial inoculum：
The Rhizopus oryzae that deposit number is CGMCC NO.15267 is inoculated in PDA culture medium, 30 DEG C, the training of 150rpm shaking flasks
Support 48h after, when thalli growth to exponential phase, thalline were collected by centrifugation by 8000rpm, with sterile water washing thalline twice after,
It is 0.5-0.6 that sterile water, which adjusts thalline OD values, and microbial inoculum is prepared.
2. pot experiment：
Use Nutrition Soil and vermiculite with volume ratio for 4:1 is mixed to get potting media, and then sterilize in 121 DEG C 1h, cooling
After dispense into potted flower pot (9cm long × 9cm wide × 9cm high), per basin about 200g.
Full grains, alfalfa seed of the same size are chosen, is put into after surface sterilization and carries out nursery in sterile soil, waited for
Germination chooses the consistent seedling progress pot experiment of growth after growing two panels true leaf, and per one seedling of basin, seedling gives birth in greenhouse
It is (25 DEG C long；12h illumination/12h dark processings), root irrigation is carried out with the 50ml microbial inoculums prepared, at isometric sterile water
As a contrast, processing is primary weekly, continuous processing 3 weeks for reason；After 2 weeks salt stress processing is carried out with 0.5% NaCl solution.In salt
After Stress treatment 30 days, plant height, root long and the plant fresh weight of each group clover are counted, the results are shown in Table 1.
Table 1：Influence of the Rhizopus oryzae to alfalfa growing under salt stress
| Processing | Plant height (cm) | Root long (cm) | Plant fresh weight (g) |
| 0% NaCl | 14.3±1.4 | 11.2±0.9 | 128.4±6.5 |
| 0% NaCl+ Rhizopus oryzae microbial inoculums | 20.6±3.5 | 16.5±2.8 | 146.6±10.2 |
| 0.5% NaCl | 5.2±1.2 | 3.8±0.6 | 43.5±3.8 |
| 0.5% NaCl+ Rhizopus oryzae microbial inoculums | 14.1±1.5 | 10.9±1.0 | 126.8±5.2 |
As can be seen from Table 1, Rhizopus oryzae using the present invention handles clover, can remarkably promote lucerne under salt stress
The growth of Mu.
Embodiment 3：Rhizopus oryzae (Rhizopus oryzae) is to the improved effect of saline-alkali soil
1. test site：It tests and is carried out in Saline-alkaline Soils of Yellow River Delta in June, 2017 in November, 2017.
2. test material：The Rhizopus oryzae (Rhizopus oryzae) of the screening separation of embodiment 1 is inoculated in wood sawdust and is trained
Support reparation matrix, the alfalfa seed that 14d is constituted.
3. experimental design：Testing ground area is one mu, and two processing are arranged, are respectively：
Processing 1：Singly apply wood sawdust；
Processing 2：Facultative Halophiles repair matrix.
4. test method：0.07 hectare is averagely divided into two cells, each cell is carried out applying improvement base by experimental design
Matter, 60 kilograms per acre.
The forward and backward pH value for measuring soil respectively is tested, and soil agreegate forms after experiment, urase, the peroxide of soil
Change hydrogen enzyme, phosphatase activity；Wherein, soil agreegate composition is measured using soil agreegate analyzer, and soil urease liveness is surveyed
Use indophenol blue colorimetry, the measurement of Activity of Catalase in Soil that volumetric method, the measurement of Activities of Phosphatase in Soil is used to adopt surely
With disodium phenyl phosphate colorimetric method.
5. test result：
The pH value that experiment soil is measured before experiment is 8.7, and after experiment, the pH value for handling 1 soil is 8.1, handles 2 soil
The pH value of earth is 7.5.
Soil urease, catalase, the phosphatase activity measured after experiment is shown in Table 2.
Table 2：Soil enzyme activities measurement result
As can be seen from Table 2, after repairing matrix using the Rhizopus oryzae of the present invention, the experimental field catalase of soil, urea
Enzyme and phosphatase activity are significantly higher than the processing for not applying microbial inoculum, show the Rhizopus oryzae microbial inoculum of the present invention to alkaline land soil
Repairing has significant effect.
As seen from Figure 1, after repairing matrix using the Rhizopus oryzae of the present invention, sample plot soil is more than 0.25mm water stabilities
Macro aggregate dramatically increases.
Test example 4：Salt stress remission effect of the Rhizopus oryzae (Rhizopus oryzae) to clover
1. test site：Shandong Agricultural University greenhouse
2. test material：The Rhizopus oryzae (Rhizopus oryzae) of the screening separation of embodiment 1 is inoculated in wood sawdust and is trained
Support the reparation matrix that 14d is constituted, alfalfa seed.
3. experimental design：Three processing are arranged in pot experiment, are respectively：CK:Normal soil, T1:Saline-alkali soil, T2：Addition
Repair the saline-alkali soil of matrix
4. test method：
CK：The normal soil of 500g is loaded per basin, plants 1g alfalfa seeds；
T1：500g moderate saline-alkali soil is loaded per basin, plants 1g alfalfa seeds；
T2：Matrix will be repaired with saline-alkali soil with 1:10 ratio is mixed, and the mixed soil of 500g, kind are loaded per basin
Plant 1g alfalfa seeds.
Measured respectively after each group alfalfa growing 4 weeks is ripe growing height, chlorophyll content, catalase (CAT) activity,
Glutathione (GSH) content；Wherein, measuring chlorophyll content use dimethyl sulfoxide method method, catalase (CAT) activity,
Glutathione (GSH) builds up kit measurement by Nanjing.
As a result as Figure 2-Figure 5, as seen from the figure, the clover of matrix group, chlorophyll content, growth are repaired in addition
Highly, catalase activity, glutathione content are significantly better than T1 groups, illustrate the Rhizopus oryzae of the present invention
(Rhizopusoryzae) salt damage symptom can be significantly slowed under salt stress, increase the biomass of clover to promote growth,
Help to improve the salt tolerance of clover.
The foregoing is merely the preferred embodiments of the application, are not intended to limit this application, for the skill of this field
For art personnel, the application can have various modifications and variations.Within the spirit and principles of this application, any made by repair
Change, equivalent replacement, improvement etc., should be included within the protection domain of the application.
SEQUENCE LISTING
<110>Shandong Agricultural University
<120>One plant of Rhizopus oryzae and its application with salt resistance ability
<130> 2018
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 590
<212> DNA
<213>Rhizopus oryzae（Rhizopus oryzae）
<400> 1
tccccctgac ttcgatcata gtttgaaagt tactggatta tactcttgta ctttacttcc 60
tgggcgaacc aaaaaaaaag atcctgagac cagcgtaata ttcctgccta gcaagccaga 120
cagaaaatca cacacatttt aggtgctcac tgtaataaaa cagcgatgcg acccatcacc 180
acataaacaa atgttatgtg tgggtttgtg atgatactga agcaggcgta ctctatagaa 240
aaaccataga gtgcaagctg cgttcaaaga ctcgatgatt cactgaatat gcaattcaca 300
ctagttatcg cactttgcta cgttcttcat cgatgcgaga accaagagat ccattgttaa 360
aagttgtttt ttattaaact ttataatact gaatttctag gtttattatg aagggtactc 420
ctgaaaccag gagtggcatc gatcaaaccc cagataggtc tacccatgac cagtctgagt 480
ctctcagcca aattttcaca gtgtagaagc aatcacttac cccagaggaa accctaaggt 540
aaggcgcttt aacataatta atgatccttc cgcaggttca cctacggaag 590
Claims (8)
1. a Rhizopus oryzae (Rhizopus oryzae), biological deposits number are：CGMCC NO.15267.
2. containing Rhizopus oryzae (Rhizopus oryzae) or/and Rhizopus oryzae (Rhizopus oryzae) described in claim 1
The microbial inoculum of metabolin.
3. microbial inoculum according to claim 2, which is characterized in that further include auxiliary material and/or carrier in the microbial inoculum.
4. microbial inoculum according to claim 2 or 3, which is characterized in that the dosage form of the microbial inoculum be liquor, emulsion, suspending agent,
Granule, pulvis, wettable powder or water dispersant.
5. following 1) -3 of Rhizopus oryzae (Rhizopus oryzae) described in claim 1) in any application：
1) application in alkaline land soil reparation；
2) application for promoting plant to be grown under salt stress environment；
3) application in preparing the biological organic fertilizer for alkaline land soil reparation.
6. following 1)-the 3 of claim 2-4 any one of them microbial inoculums) any application in：
1) application in alkaline land soil reparation；
2) application for promoting plant to be grown under salt stress environment；
3) application in preparing the biological organic fertilizer for alkaline land soil reparation.
7. a kind of preparation for alkaline land soil reparation, active constituent is Rhizopus oryzae (Rhizopus described in claim 1
Oryzae), zymotic fluid, bacteria suspension and/or the supernatant of Rhizopus oryzae (Rhizopus oryzae).
8. a kind of method of alkaline land soil reparation, which is characterized in that be by biological deposits number：The rice root of CGMCC NO.15267
Zymotic fluid, cellular lysate liquid or the spore suspension of mould (Rhizopus oryzae) are sprayed in alkaline land soil；Or by right
It is required that 1 Rhizopus oryzae (Rhizopus oryzae) is inoculated into wood sawdust and cultivates, pH to 7.0 is adjusted, 7-14 is cultivated at 30 DEG C
It, the wood sawdust that then will be inoculated with Rhizopus oryzae (Rhizopus oryzae) applies in alkaline land soil.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114164126A (en) * | 2022-01-17 | 2022-03-11 | 山东农业大学 | Rhizopus oryzae XERF-1 and application thereof in relieving apple continuous cropping obstacle |
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| CN114164126B (en) * | 2022-01-17 | 2023-05-26 | 山东农业大学 | Rhizopus oryzae XERF-1 and application thereof in relieving apple continuous cropping obstacle |
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