CN108753594A - A kind of high throughput biological sample grinding device and its grinding method - Google Patents
A kind of high throughput biological sample grinding device and its grinding method Download PDFInfo
- Publication number
- CN108753594A CN108753594A CN201810929653.6A CN201810929653A CN108753594A CN 108753594 A CN108753594 A CN 108753594A CN 201810929653 A CN201810929653 A CN 201810929653A CN 108753594 A CN108753594 A CN 108753594A
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- China
- Prior art keywords
- lid
- pipe
- fixed strip
- tube column
- tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
Abstract
The present invention relates to a kind of high-throughput biological sample grinding device and its grinding method,The device includes tube column,Pipe fixed strip,Upper cover,Lid fixed strip and reinforcing device,The tube column is top end opening,Upper coarse and lower fine hollow round table structure,The quantity of the tube column is eight,Eight tube columns are arranged side by side and are attached eight tube columns by pipe fixed strip,The pipe fixed strip is arranged in the middle part upper position of tube column,The present invention can be used with mating pedestal,Single-base can the row of 8 row of once-through operation × 12 totally 96 centrifuge tubes,Therefore,Once operation can ground sample 192 on general beveller,Than tradition 3 times or so are improved using single 1.5ml EP pipe operating efficiencies,Eight tube columns are attached fixation by pipe fixed strip,Connecting leg in centrifugally operated is avoided first to be broken under the action of the centrifugal force,Secondly eight tube columns are indeformable after heating,Convenient for the addition of follow-up Multi-channel liquid transfer device and draw solution.
Description
Technical field
The present invention relates to Bioexperiment engineering device technique field more particularly to a kind of high-throughput biological sample grinding device and
Grinding method.
Background technology
In biological experiment, the broken operation of biochemical reaction a small amount of in centrifuge tube and biological tissue is frequently involved,
For example be used for subsequent biological experiment after blade, seed etc. being crushed in historrhexis, which part subsequent experimental needs to exist
Centrifugally operated is carried out in centrifuge.Common practice is managed using single 1.5ml EP at present, after biological tissue and steel ball are added
It is ground, centrifugally operated, this method takes time and effort the disadvantage is that inefficiency, of high cost.It can therefore, it is necessary to develop one kind
Biochemical reaction, grinding centrifugal method and the grinding device of high throughput operation.
Invention content
In view of this, the present invention provides a kind of high-throughput biological sample grinding device, main purpose is to solve current life
Object sample tissue is broken, sample grinding, addition, draw solution and centrifugal process inefficiency in centrifugal process, it is difficult to batch
The problem of operation.
In order to solve the above technical problems, the technical solution adopted in the present invention is：
A kind of high throughput biological sample grinding device, including tube column (6), pipe lid (1), pipe fixed strip (5), lid are fixed
Item (2) and lid reinforcing device (7)；The tube column (6) be top end opening, upper coarse and lower fine hollow round table structure, it is described
The quantity of tube column (6) is eight, and eight tube columns (6) are arranged side by side and are attached by pipe fixed strip (5), described
Pipe fixed strip (5) setting is correspondingly arranged on pipe above the middle part upper position of tube column (6), each tube column (6)
Lid (1), the pipe lid (1) are attached with tube column (6) by connection strap (4), and eight pipe lids (1) pass through lid
Fixed strip (2) links together, and the both ends of the lid fixed strip (2) are equipped with the extension (3) extended outward, the pipe
The upper surface of lid (1) is equipped with the groove to lower recess, can be with lid reinforcing device (7) with the use of realization pipe lid (1)
It reinforces.
Further, the outer rim of sealing part outwardly protrudes below the pipe lid (1), by the outer rim of protrusion by pipe lid
(1) it is fastened on tube column (6) oral area.
Further, the lower end of the reinforcing device (7) be provided with eight it is equal with the groove diameter of pipe lid (1) upper surface
Lug boss (8), in the corresponding groove that be located in eight pipe lid (1) surfaces of eight lug bosses (8).
Further, the tube column and pipe lid are polyethylene material.
In addition, the method being ground using the high-throughput biological sample grinding device, tool is also claimed in the present invention
Body includes the following steps：
(1) eight pipe lids (1) are opened by starting the extension (3) at lid fixed strip (2) both ends；
(2) biological sample to be ground and steel ball are added in eight tube columns (6) simultaneously；
(3) eight pipe lids (1) are covered, are reinforced with lid reinforcing device (7), eight connecting legs are then put into beveller
It is ground after pedestal.
Compared with prior art, high-throughput biological sample grinding device of the invention has following advantageous effect：
1, the present invention can be used with mating pedestal, single-base can 8 row of once-through operation × 12 arrange totally 96 centrifuge tubes,
Therefore, on general beveller once operation can ground sample 192, carried using single 1.5ml EP pipe operating efficiencies than tradition
High 3 times or so；
2, eight tube columns are attached fixation by pipe fixed strip, avoid in centrifugally operated connecting leg first in centrifugal force
The lower fracture of effect, eight tube columns are indeformable after secondly heating, convenient for the addition of follow-up Multi-channel liquid transfer device and draw solution；
3, the single tube circle center distance of 8 connecting legs can be used for common 8 channel in laboratory or 12 channel Eppendorf pipettors
And other brand Multi-channel liquid transfer devices, therefore can once to 8 either 12 centrifuge tube solubilization liquid or draw centrifugation after
Supernatant avoids traditional single centrifuge tube operation；
4, the centrifugation with high-throughput hanging basket rotor that the device can be common in laboratories such as Eppendorf 5810 (R)
It is centrifuged on machine, therefore the centrifugally operated on 4 pedestals 96 × 4 totally 384 samples, the single 1.5ml of tradition may be implemented in single
The once centrifugation only 24 or 30 of centrifuge tube centrifugation；
5, it interconnects and is connected to form one structure with eight tube columns between eight pipe lids, and tip designs are convex
Go out extension, convenient for eight pipe lids are once opened and closed operation, improves operating efficiency；
Problem, present apparatus design are easily loosened when 6, centrifuging for conventional centrifugal pipe or shake lid stress in shattering process
Lid reinforcing device, lid using the pushing of lid reinforcing device can reinforce lid after covering, prevent lid in experimentation from loosening.
Above description is only the general introduction of technical solution of the present invention, in order to better understand the technical means of the present invention,
And can be implemented in accordance with the contents of the specification, below with presently preferred embodiments of the present invention and after coordinating attached drawing to be described in detail such as.
Description of the drawings
It is of the invention to reach the technological means and effect that predetermined goal of the invention is taken further to illustrate, below in conjunction with
Specific implementation mode, structure, feature and its effect applied according to the present invention is described in detail such as in attached drawing and preferred embodiment
Afterwards.In the following description, what different " embodiment " or " embodiment " referred to is not necessarily the same embodiment.In addition, one or more
Special characteristic, structure or feature in a embodiment can be combined by any suitable form.
Fig. 1 is the company of a kind of high-throughput biological sample grinding device eight tube columns and eight pipe lids provided by the invention
Connect relational graph；
Fig. 2 is a kind of structural schematic diagram of high-throughput biological sample grinding device reinforcing device provided by the invention；
Fig. 3 is a kind of integral installation structural schematic diagram of high-throughput biological sample grinding device provided by the invention.
The reference numerals are as follows：
1, pipe lid；2, lid fixed strip；3, extension；4, connection strap；5, pipe fixed strip；6, tube column；7, reinforcing device；8,
Lug boss.
Specific implementation mode
To make the object, technical solutions and advantages of the present invention clearer, technical scheme of the present invention will be carried out below
Detailed description.Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are obtained all without making creative work
Other embodiment belongs to the range that the present invention is protected.
With reference to figure 1-3, the present invention provides a kind of high-throughput biological sample grinding device, including tube column 6, pipe lid 1,
Pipe fixed strip 5, lid fixed strip 2 and reinforcing device 7, the tube column 6 are top end opening, upper coarse and lower fine hollow round table knot
The quantity of structure, the tube column 6 is eight, and eight tube columns 6 are arranged side by side and are attached by pipe fixed strip 5, this
The purpose of sample setting is to be reinforced to eight tube columns 6 so that during grinding, prevents tube column 6 from becoming
Shape or tearing, the pipe fixed strip 5 are arranged in the middle part upper position of tube column 6, and 6 top of each tube column is corresponding
It is provided with pipe lid 1, the pipe lid 1 is attached with the tube column 6 by connection strap 4, wherein connection strap 4 is soft
Polyethylene material, pipe lid 1 is linked together with corresponding tube column 6, prevent pipe lid 1 lose, eight pipe lids
Son 1 is linked together by lid fixed strip 2, and the both ends of the lid fixed strip 2 are equipped with the extension 3 extended outward, profit
Can facilitate staff quickly by eight pipe lids 1 with the extension 3 while open, simple operation, the pipe lid 1 it is upper
Surface is equipped with the groove to lower recess, can be with lid reinforcing device 7 with the use of the reinforcing for realizing pipe lid 1, the reinforcing device 7
Lower end be provided with eight lug bosses 8 equal with the groove diameter of 1 upper surface of pipe lid, eight of 7 lower section of the reinforcing device
Lug boss 8 corresponds in the groove that be located in eight 1 surfaces of pipe lid.
Further, the outer rim of 1 lower section sealing part of the pipe lid outwardly protrudes, by the outer rim of protrusion by pipe lid 1
It is fastened on 6 oral area of tube column, can realize the fast fastening between pipe lid 1 and tube column 6, it is easy to operate.
Further, the tube column 6 and pipe lid 1 are polyethylene material.
The application method of the grinding device is：
(1) eight pipe lids (1) are opened by starting the extension (3) at lid fixed strip (2) both ends；
(2) biological sample to be ground and steel ball are added in eight tube columns (6) simultaneously；
(3) eight pipe lids (1) are covered, are reinforced with lid reinforcing device (7), eight connecting legs are then put into beveller
It is ground after pedestal, or is put into centrifuge and carries out centrifugally operated.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (5)
1. a kind of high throughput biological sample grinding device, it is characterised in that：Including tube column (6), pipe lid (1), pipe fixed strip
(5), lid fixed strip (2) and lid reinforcing device (7)；The tube column (6) is top end opening, upper coarse and lower fine hollow round table
The quantity of structure, the tube column (6) is eight, and eight tube columns (6) are arranged side by side and are carried out by pipe fixed strip (5)
Connection, pipe fixed strip (5) setting are corresponding above the middle part upper position of tube column (6), each tube column (6)
It is provided with pipe lid (1), the pipe lid (1) is attached with tube column (6) by connection strap (4), eight pipe lids
(1) it is linked together by lid fixed strip (2), and the both ends of the lid fixed strip (2) are equipped with the extension extended outward
(3), the upper surface of the pipe lid (1) is equipped with the groove to lower recess, can be managed with the use of realization with lid reinforcing device (7)
The reinforcing of lid (1).
2. a kind of high-throughput biological sample grinding device according to claim 1, which is characterized in that the pipe lid (1)
The outer rim of lower section sealing part outwardly protrudes, and upper cover (1) is fastened on tube column (6) oral area by the outer rim of protrusion.
3. a kind of high-throughput biological sample grinding device according to claim 1, which is characterized in that the reinforcing device (7)
Lower end be provided with eight lug bosses (8) equal with the groove diameter of pipe lid (1) upper surface, eight lug bosses (8) are corresponding
In the groove that be located in eight pipe lid (1) surfaces.
4. a kind of high-throughput biological sample grinding device according to claim 1, which is characterized in that the tube column (6)
It is polyethylene material with pipe lid (1).
5. a kind of method being ground using any one of the claim 1-4 high-throughput biological sample grinding devices, special
Sign is, includes the following steps：
(1) eight pipe lids (1) are opened by starting the extension (3) at lid fixed strip (2) both ends；
(2) biological sample to be ground and steel ball are added in eight tube columns (6) simultaneously；
(3) eight pipe lids (1) are covered, are reinforced with lid reinforcing device (7), eight connecting legs are then put into beveller pedestal
After be ground.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810929653.6A CN108753594A (en) | 2018-08-15 | 2018-08-15 | A kind of high throughput biological sample grinding device and its grinding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810929653.6A CN108753594A (en) | 2018-08-15 | 2018-08-15 | A kind of high throughput biological sample grinding device and its grinding method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108753594A true CN108753594A (en) | 2018-11-06 |
Family
ID=63970099
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810929653.6A Pending CN108753594A (en) | 2018-08-15 | 2018-08-15 | A kind of high throughput biological sample grinding device and its grinding method |
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| CN (1) | CN108753594A (en) |
Citations (11)
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| CN202823544U (en) * | 2012-10-26 | 2013-03-27 | 中国农业科学院农业资源与农业区划研究所 | Rapid grinding device |
| CN204503131U (en) * | 2015-02-07 | 2015-07-29 | 靖江市华煜阳光医疗器械有限公司 | A kind of multiple-connecting-pipe |
| CN205170824U (en) * | 2015-12-09 | 2016-04-20 | 上海兰卫医学检验所股份有限公司 | Eight ally oneself with row's lid |
| CN106237858A (en) * | 2016-08-04 | 2016-12-21 | 王尧 | A kind of high flux hemodialysis device |
| CN206232719U (en) * | 2016-11-23 | 2017-06-09 | 湖北省农业科学院粮食作物研究所 | A kind of high flux extracting genome DNA device |
| CN206355685U (en) * | 2017-01-04 | 2017-07-28 | 四川农业大学 | A kind of high throughput filtration device of experiment |
| CN207047233U (en) * | 2017-05-26 | 2018-02-27 | 遵义医学院附属医院 | A kind of new PCR pipe lid and supporting orifice plate |
| CN207498363U (en) * | 2017-11-16 | 2018-06-15 | 南京先进激光技术研究院 | A kind of PCR detection kit box cover |
| CN208748098U (en) * | 2018-08-15 | 2019-04-16 | 湖北省农业科学院粮食作物研究所 | A kind of high throughput biological sample grinding device |
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2018
- 2018-08-15 CN CN201810929653.6A patent/CN108753594A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000074877A1 (en) * | 1999-06-07 | 2000-12-14 | Nicast Ltd. | Filtering material and device and method of its manufacture |
| US20070025895A1 (en) * | 2005-07-27 | 2007-02-01 | Zunshe Qin | Protein crystallography dialysis chamber that enables off-site high throughput cocktail screen |
| CN202823544U (en) * | 2012-10-26 | 2013-03-27 | 中国农业科学院农业资源与农业区划研究所 | Rapid grinding device |
| CN204503131U (en) * | 2015-02-07 | 2015-07-29 | 靖江市华煜阳光医疗器械有限公司 | A kind of multiple-connecting-pipe |
| CN205170824U (en) * | 2015-12-09 | 2016-04-20 | 上海兰卫医学检验所股份有限公司 | Eight ally oneself with row's lid |
| CN106237858A (en) * | 2016-08-04 | 2016-12-21 | 王尧 | A kind of high flux hemodialysis device |
| CN206232719U (en) * | 2016-11-23 | 2017-06-09 | 湖北省农业科学院粮食作物研究所 | A kind of high flux extracting genome DNA device |
| CN206355685U (en) * | 2017-01-04 | 2017-07-28 | 四川农业大学 | A kind of high throughput filtration device of experiment |
| CN207047233U (en) * | 2017-05-26 | 2018-02-27 | 遵义医学院附属医院 | A kind of new PCR pipe lid and supporting orifice plate |
| CN207498363U (en) * | 2017-11-16 | 2018-06-15 | 南京先进激光技术研究院 | A kind of PCR detection kit box cover |
| CN208748098U (en) * | 2018-08-15 | 2019-04-16 | 湖北省农业科学院粮食作物研究所 | A kind of high throughput biological sample grinding device |
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