CN108741088A - Heat-resisting probiotic composition and health food comprising the composition - Google Patents
Heat-resisting probiotic composition and health food comprising the composition Download PDFInfo
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- CN108741088A CN108741088A CN201810528397.XA CN201810528397A CN108741088A CN 108741088 A CN108741088 A CN 108741088A CN 201810528397 A CN201810528397 A CN 201810528397A CN 108741088 A CN108741088 A CN 108741088A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/119—Bifermentans
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/147—Helveticus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/533—Longum
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Molecular Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention provides a kind of heat-resisting probiotic composition and the health food comprising the composition.The heat-resisting probiotic composition includes by mass percentage：The bifidobacterium longum of 30%-40%, the Lactobacillus helveticus of 20%-40%, the lactobacillus fermenti of 5%-10%, the fruit of Chinese wolfberry of 6%-10%, 10%-20% soyabean oligosaccharides.This heat-resisting probiotic composition can improve enteric microorganism environment, treatment constipation caused by intestinal flora is lacked of proper care, it is rapid-action, significant effect, the immunocompetence of human body can also be improved, and the heat-resisting probiotics has high resistance to cold and diseases, when applying it in health food, the destruction that hot environment and food in food manufacturing process enter digestive ferment after gastrointestinal tract can be effectively antagonized, the edible health food comprising the probiotic composition can obviously improve the composition of flora in host intestine, prevention and improve constipation, it is without side-effects, it deactivates and does not rebound.
Description
Technical field
The invention belongs to composition and field of health care food, and in particular to a kind of heat-resisting probiotic composition and include the group
Close the health food of object.
Background technology
Recent studies indicate that the bacterium in human body, up to more than 1000 kind, especially the bacterium in gastrointestinal tract is the richest
Richness accounts for whole body micro-ecological bacterial sum 80% or so, is 10 times of human body cell, is known as " the second genome " of the mankind.These
Bacterium contributes to human consumption's food, generates vitamin to prevent the disease that bacterium in food is induced, while stimulating siberian crabapple
System.With going deep into for research, enteric microorganism is found in many chronic diseases and symptom, such as inflammation, obesity, diabetes, is gone back
There is cancer etc. to also play key effect.The quantity and gene dosage of microorganism are surprising, the strong health for influencing the mankind.These find
Application for probiotic products in fields such as food, health products, medicine, cultivation, environment, amenities brings huge promotion
Power.Currently, probiotics is widely used in the products such as Yoghourt, sour milk beverage, probiotics powder agent, animal feed, environmental improvement,
Form relatively complete and ripe probiotics industry.
The premise that probiotics in health food plays human body adjustment effect is：1. the high survival in the health products term of validity
Rate；2. ensureing certain survival rate in gastrointestinal tract after entering human body.However, in the making of health food especially bakery
In the process, high-temperature heating is inevitably had；Food enter digestion also can inevitably be subjected to after hydrochloric acid in gastric juice and some
The destruction of protease, this just proposes high requirement to the quantity of probiotics and resistance.CN105105104A discloses heat-resisting
Probiotic composition and healthy food comprising the composition, but thermoduric bacteria manufacturing process is cumbersome, fringe cost is high, it is difficult to it promotes
Using.
Constipation is a kind of common gastrointestinal symptom, refers to intestinal contents bradykinesia in the intestine, excrement is in colon
Retention time is long, moisture reduces, excrement is dry, it is hard, measure and less and be not easy caused by discharge.After constipation can make various decomposition
Waste and harmful substance drainage difficulty, with the passing of time can cause hemorrhage of digestive tract, polyp or even tumour.Constipation or modern are common
Disease will cause many disease of digestive tracts, very once waste will be made to be deposited in enteron aisle for a long time if suffering from and being not treated in time
To causing various skin problems.Clinically commonly not only treatment cycle is generally partially long for the drug for the treatment of constipation at present, Bu Nengji
When effective relief of constipation patient the problem of, and exist be discontinued knock-on the phenomenon that.CN107319567A discloses a kind of energy rush
Into the compound probiotic chewable tablets of Instestinal motility；CN107095308A discloses a kind of probiotics preparation and its application, this is prebiotic
Bacteria preparation can improve enteric microorganism environment, treatment constipation caused by intestinal flora is lacked of proper care, rapid-action, significant effect.
But if applying it to field of food, there is no solve for the degeneration-resistant sex chromosome mosaicism of probiotics.
Invention content
To solve the above problems, the present invention provides a kind of heat-resisting probiotic composition and the health care comprising the composition food
Product, of the invention first purpose are to provide a kind of composition of heat-resisting probiotics.
First purpose of the present invention is achieved through the following technical solutions：
A kind of heat-resisting benefit being made of bifidobacterium longum, Lactobacillus helveticus, lactobacillus fermenti, the fruit of Chinese wolfberry and soyabean oligosaccharides
Raw bacteria composition.
Preferably, each component is by mass percentage in the heat-resisting probiotic composition：The long bifid bar of 30%-40%
Bacterium, the Lactobacillus helveticus of 20%-40%, the lactobacillus fermenti of 5%-10%, the fruit of Chinese wolfberry of 6%-10%, 10%-20% it is big
Beans oligosaccharide.
Preferably, each component is by mass percentage in the heat-resisting probiotic composition：35% bifidobacterium longum,
34% Lactobacillus helveticus, 8% lactobacillus fermenti, 8% fruit of Chinese wolfberry, 15% soyabean oligosaccharides.
The present invention also provides the preparation methods of above-mentioned heat-resisting probiotics：
The preparation method of bifidobacterium longum is：
A. the preparation of culture medium：75% MRS, 1% cysteine hydrochloride, 1% dimension are weighed by weight percentage
Raw element C, 5% carrot juice, 10% lentinan, 8% soya-bean milk prepare culture medium；
B. Cellulose/Chitosan gel, gel porosity 85% are added in the medium, mean pore size is
450nm.By a concentration of 109The bifidobacterium longum bacterium solution of CFU/mL is inoculated into culture medium, and inoculation bacterium solution amount is 25%.Anaerobism is trained
It supports, after 37 DEG C of culture 16h, obtains the gel particle of bifidobacterium longum aggregation.
The preparation method of Lactobacillus helveticus is：
A. the preparation of culture medium：Weigh 30% Medium of Bifidobacterium by weight percentage, 30% MRS culture mediums,
20% YPD culture mediums, 5% inulin, 5% yeast dextran, 5% whey fermentation liquid, 3% tomato juice, 2% winter
Worm summer grass polysaccharide prepares culture medium；
Cellulose aerogels material is added in culture medium, cellulose aerogels material porosity is 99%, and average pore size is big
Small 50nm.By a concentration of 109The Lactobacillus helveticus of CFU/mL is inoculated into culture medium, after 37 DEG C of culture 36h, fibril aggregation body surface
Face and the internal a large amount of Lactobacillus helveticus of aggregation.
The preparation method of lactobacillus fermenti is：
A. the preparation of culture medium：Weigh 85% YPD by weight percentage, 4% malt polysaccharide, 1% vitamin,
5% mushroom polysaccharide, 5% ganoderma lucidum polysaccharide prepare culture medium；
B. Polylactic acid foam material is added in culture medium, Polylactic acid foam material porosity is 95%, mean pore size
It is 50 μm.Then by a concentration of 108CFU/mL lactobacillus fermentis are inoculated into culture medium, Anaerobic culturel, after 30 DEG C of cultures for 24 hours,
Foam surface and internal aggregation bulk fermentation lactobacillus.
Bifidobacterium longum, Lactobacillus helveticus, lactobacillus fermenti are bought in the management of Chinese industrial Microbiological Culture Collection
The heart.
Every gram of heat-resisting probiotic composition provided by the invention is 120 hundred million -130 hundred million, preferably 12,000,000,000 containing viable count.
Second object of the present invention is to provide the health food of the composition containing above-mentioned heat-resisting probiotics.
The health food is flour product, dairy products, drink, fruit juice or leisure snacks, and the health food is by common
Method for making food is process, and containing condiment, additive and colorant, the preservative etc. contained by ordinary food, is being processed
During above-mentioned heat-resisting probiotic composition is added.
Investigation discovery is carried out to the viable count in the health food containing the heat-resisting probiotics：On the one hand, by food
Viable count declines unobvious after manufacturing process machine shearing force and baking, and viable count can reach 80% or more, and by extremely
After long-term storage in few 6 months, also unobvious, viable count can reach 90% or more for viable count decline；On the other hand, when food into
Enter human body after digestive system, viable count declines unobvious, and viable count can reach 80% or more.
This health food can be used for improving enteric microorganism environment, pass through the defecation situation to eating health food crowd
Investigation discovery is carried out, can obviously improve constipation situation after eating the health food, and can obviously improve body immunity.
Composition provided by the invention containing heat-resisting probiotics and the health food containing the composition can obviously improve
The constipation situation of constipation crowd, it is safely and effectively, and quick, without side-effects, be suitble to crowd wide, have deactivate do not rebound it is excellent
Gesture.
Probiotics in the heat-resisting probiotic composition has high resistance to cold and diseases, ensure that probiotics plays human body opsonic action
When requirement to probiotics quantity, play health-care effect for health food and provide guarantee.
Specific embodiment
In order to make those skilled in the art fully understand the present invention, this hair is further illustrated below by way of specific embodiment
It is bright, but those skilled in the art should know that the embodiment of the present invention is not limit the invention in any way.
Composition embodiment 1
Heat-resisting probiotic composition provided in this embodiment includes by mass percentage：Bifidobacterium longum 35%, Switzerland
Lactobacillus 34%, lactobacillus fermenti 8%, the fruit of Chinese wolfberry 8%, soyabean oligosaccharides 15%, wherein every gram of heat-resisting probiotic composition
In, viable count is 12,000,000,000.
Composition embodiment 2
Heat-resisting probiotic composition provided in this embodiment includes by mass percentage：Bifidobacterium longum 35%, Switzerland
Lactobacillus 34%, lactobacillus fermenti 8%, the fruit of Chinese wolfberry 8%, soyabean oligosaccharides 15%, wherein every gram of heat-resisting probiotic composition
In, viable count is 12,500,000,000.
Composition embodiment 3
Heat-resisting probiotic composition provided in this embodiment includes by mass percentage：Bifidobacterium longum 35%, Switzerland
Lactobacillus 34%, lactobacillus fermenti 8%, the fruit of Chinese wolfberry 8%, soyabean oligosaccharides 15%, wherein every gram of heat-resisting probiotic composition
In, viable count is 13,000,000,000.
Composition embodiment 4
Heat-resisting probiotic composition provided in this embodiment includes by mass percentage：Bifidobacterium longum 30%, Switzerland
Lactobacillus 40%, lactobacillus fermenti 10%, the fruit of Chinese wolfberry 6%, soyabean oligosaccharides 14%, wherein every gram of heat-resisting probiotic composition
In, viable count is 12,000,000,000.
Composition embodiment 5
Heat-resisting probiotic composition provided in this embodiment includes by mass percentage：Bifidobacterium longum 30%, Switzerland
Lactobacillus 40%, lactobacillus fermenti 10%, the fruit of Chinese wolfberry 6%, soyabean oligosaccharides 14%, wherein every gram of heat-resisting probiotic composition
In, viable count is 13,000,000,000.
Composition embodiment 6
Heat-resisting probiotic composition provided in this embodiment includes by mass percentage：Bifidobacterium longum 40%, Switzerland
Lactobacillus 20%, lactobacillus fermenti 10%, the fruit of Chinese wolfberry 10%, soyabean oligosaccharides 20%, wherein every gram of heat-resisting probiotic composition
In, viable count is 12,000,000,000.
Composition embodiment 7
Heat-resisting probiotic composition provided in this embodiment includes by mass percentage：Bifidobacterium longum 40%, Switzerland
Lactobacillus 20%, lactobacillus fermenti 10%, the fruit of Chinese wolfberry 10%, soyabean oligosaccharides 20%, wherein every gram of heat-resisting probiotic composition
In, viable count is 13,000,000,000.
Composition embodiment 8
Heat-resisting probiotic composition provided in this embodiment includes by mass percentage：Bifidobacterium longum 30%, Switzerland
Lactobacillus 30%, lactobacillus fermenti 10%, the fruit of Chinese wolfberry 10%, soyabean oligosaccharides 20%, wherein every gram of heat-resisting probiotic composition
In, viable count is 12,000,000,000.
Composition embodiment 9
Heat-resisting probiotic composition provided in this embodiment includes by mass percentage：Bifidobacterium longum 40%, Switzerland
Lactobacillus 34%, lactobacillus fermenti 10%, the fruit of Chinese wolfberry 6%, soyabean oligosaccharides 10%, wherein every gram of heat-resisting probiotic composition
In, viable count is 12,000,000,000.
Composition embodiment 10
Heat-resisting probiotic composition provided in this embodiment includes by mass percentage：Bifidobacterium longum 35%, Switzerland
Lactobacillus 40%, lactobacillus fermenti 5%, the fruit of Chinese wolfberry 10%, soyabean oligosaccharides 10%, wherein every gram of heat-resisting probiotic composition
In, viable count is 12,000,000,000.
Composition embodiment 11
Heat-resisting probiotic composition provided in this embodiment includes by mass percentage：Bifidobacterium longum 35%, Switzerland
Lactobacillus 30%, lactobacillus fermenti 5%, the fruit of Chinese wolfberry 10%, soyabean oligosaccharides 20%, wherein every gram of heat-resisting probiotic composition
In, viable count is 12,000,000,000.
Comparative example 1
Heat-resisting probiotic composition provided in this embodiment includes by mass percentage：Bifidobacterium longum 40%, Switzerland
Lactobacillus 40%, lactobacillus fermenti 20%, in every gram of heat-resisting probiotic composition, viable count is 12,000,000,000.
Comparative example 2
Composition provided in this embodiment includes the fruit of Chinese wolfberry 40% and soyabean oligosaccharides 60% by mass percentage.
Effect example
1. case selection：
This group of case be because intestinal flora lack of proper care caused by constipation patient, meet be included in, exclusion criteria, the age 10-
Between 70 years old, wherein man accounts for 50%, Ms and accounts for 50%.13 groups are randomly divided into, every group each 30, wherein test group is 1-
11 groups, control group is the 12nd and 13 group.There was no significant difference for the data such as age, gender, the state of an illness of 1-13 group patients, has comparable
Property.
2. therapeutic scheme：
1-11 groups (test group) patient applies the composition that composition embodiment 1-11 is provided respectively, once a day,
Each 1.0g；The composition that 12nd and the 13rd group (control group) applies comparative example l and comparative example 2 is provided, daily
Once, each 1.0g.
It observes and records：Observe the state of an illness after medication a period of time, and according to " significant effect ', " effective ", engineering noise " evil
Change " this four grade evaluates the therapeutic effect of the medicament.
3. clinical evaluation standard：
Significant effect：It takes medicine after a period of time, patient 2 times/day of l-of stool, just matter is soft.
Effectively：It takes medicine after a period of time, patient defecates 2 once, and just matter is prayed continuing to take medicine close to normally.
In vain：It takes medicine after a period of time, patient defecates situation without significant change.
Deteriorate：It takes medicine after a period of time, other adverse reactions occurs in patient.
4. being observed after taking 10 days, the results are shown in Table 1：
1 clinical test results of table
| Project | Significant effect (example) | Effectively (example) | (example) in vain | Deteriorate (example) |
| Test group 1 | 29 | 1 | 0 | 0 |
| Test group 2 | 28 | 2 | 0 | 0 |
| Test group 3 | 27 | 3 | 0 | 0 |
| Test group 4 | 27 | 3 | 0 | 0 |
| Test group 5 | 26 | 4 | 0 | 0 |
| Test group 6 | 27 | 3 | 0 | 0 |
| Test group 7 | 26 | 4 | 0 | 0 |
| Test group 8 | 26 | 4 | 0 | 0 |
| Test group 9 | 26 | 4 | 0 | 0 |
| Test group 10 | 27 | 3 | 0 | 0 |
| Test group 11 | 27 | 3 | 0 | 0 |
| Control group 1 | 0 | 30 | 0 | 0 |
| Control group 2 | 0 | 20 | 10 | 0 |
Control group l continues to take the heat-resisting probiotic composition that comparative example 1 is provided, once a day, each 1.0g,
It is effective to have 28, effective 2 after 1 month；Control group 2 continues to take the combination that comparative example 2 is provided
Object, once a day, each 1.0g are effective to have 27, effective 3 after 2 months.
Test group 1-11 patient takes after 10 days symptoms improve and cuts out one month, free movement of the bowels can be still kept, without anti-
Play phenomenon.
In conjunction with table 1 and stop observation post administration it is found that when heat-resisting probiotic composition provided in an embodiment of the present invention treats constipation
Not only significant effect, treatment time is short, and with the deactivated advantage not rebounded.
1 probiotic biscuit of health food embodiment
A. biscuit ingredient
The probiotic biscuit is mixed by 1% heat-resisting probiotic composition in the dispensing of common biscuit, by common biscuit
Production method bakes.
B. microorganism survival rate is investigated
Maximum temperature is about 200 DEG C, baking process 10 minutes, is suitble to most of industrial biscuit productions, micro- life after baking
Object survival rate is 85%.
2 probiotics bread of health food embodiment
A. bread component
The probiotics bread is mixed by 1% probiotic composition in the dispensing of common bread, by the making of common bread
Method bakes.
B. microorganism survival rate is investigated
Maximum temperature is about 200 DEG C, baking process 10 minutes, is suitble to most of industrial bread productions, micro- life after baking
Object survival rate is 83%.
Dairy products, drink, fruit juice or leisure snacks can be made by above-mentioned similar approach and measure its Microbial survival rate.
Microbial survival rate study on the stability
A. time factor influences it
After above-mentioned health food is preserved certain time (6 months), its Microbial survival rate is measured again in 90%-95%
Between.
B. environmental factor influences it
The heat-resisting probiotic composition of the present invention is put into artificial simulation gastric juices and after intestinal juice processing a period of time, is measured micro-
Biological survival rate is between 80%-90%.
Claims (10)
1. a kind of heat-resisting probiotic composition, it is characterised in that the heat-resisting probiotic composition is by bifidobacterium longum, Switzerland's breast bar
Bacterium, lactobacillus fermenti, the fruit of Chinese wolfberry and soyabean oligosaccharides composition.
2. heat-resisting probiotic composition as described in claim 1, which is characterized in that the heat-resisting probiotic composition each component is pressed
Mass percent is calculated as：The bifidobacterium longum of 30%-40%, the Lactobacillus helveticus of 20%-40%, 5%-10% acidified milk bar
Bacterium, the fruit of Chinese wolfberry of 6%-10%, 10%-20% soyabean oligosaccharides.
3. heat-resisting probiotic composition as claimed in claim 2, which is characterized in that the heat-resisting probiotic composition each component is pressed
Mass percent is calculated as：35% bifidobacterium longum, 34% Lactobacillus helveticus, 8% lactobacillus fermenti, 8% fruit of Chinese wolfberry,
15% soyabean oligosaccharides.
4. heat-resisting probiotic composition as described in claim 1, which is characterized in that the preparation method of bifidobacterium longum is：
A. the preparation of culture medium：75% MRS, 1% cysteine hydrochloride, 1% vitamin are weighed by weight percentage
C, 5% carrot juice, 10% lentinan, 8% soya-bean milk prepare culture medium；
B. Cellulose/Chitosan gel, gel porosity 85%, mean pore size 450nm are added in the medium；It will
A concentration of 109The bifidobacterium longum bacterium solution of CFU/mL is inoculated into culture medium, and inoculation bacterium solution amount is 25%；Anaerobic culturel, 37 DEG C
After cultivating 16h, the gel particle of bifidobacterium longum aggregation is obtained.
5. heat-resisting probiotic composition as described in claim 1, which is characterized in that the preparation method of Lactobacillus helveticus is：
A. the preparation of culture medium：Weigh 30% Medium of Bifidobacterium by weight percentage, 30% MRS culture mediums, 20%
YPD culture mediums, 5% inulin, 5% yeast dextran, 5% whey fermentation liquid, 3% tomato juice, 2% worm summer in winter
Grass polysaccharide prepares culture medium；
B. cellulose aerogels material is added in culture medium, cellulose aerogels material porosity is 99%, mean pore size
For 50nm；By a concentration of 109The Lactobacillus helveticus of CFU/mL is inoculated into culture medium, after 37 DEG C of culture 36h, fibril aggregation body surface
Face and the internal a large amount of Lactobacillus helveticus of aggregation.
6. heat-resisting probiotic composition as described in claim 1, which is characterized in that the preparation method of lactobacillus fermenti is：
A. the preparation of culture medium：Weigh 85% YPD by weight percentage, 4% malt polysaccharide, 1% vitamin, 5%
Mushroom polysaccharide, 5% ganoderma lucidum polysaccharide prepare culture medium；
B. Polylactic acid foam material is added in culture medium, Polylactic acid foam material porosity is 95%, and mean pore size is 50 μ
m；Then by a concentration of 108CFU/mL lactobacillus fermentis are inoculated into culture medium, Anaerobic culturel, after 30 DEG C of cultures for 24 hours, foam table
Face and internal aggregation bulk fermentation lactobacillus.
7. heat-resisting probiotic composition according to claim 1, which is characterized in that every gram of heat-resisting probiotic composition
In, viable count is 12,000,000,000-130 hundred million.
8. heat-resisting probiotic composition according to claim 7, which is characterized in that every gram of heat-resisting probiotic composition
In, viable count is 12,000,000,000.
9. the heat-resisting probiotic composition of claim 1-8 any one of them is preparing the guarantor for improving enteric microorganism environment
Application in health food.
10. health food as claimed in claim 9, which is characterized in that the health food be flour product, dairy products, drink,
Any one of fruit juice, leisure snacks.
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