CN108740783A - The highland barley germination technology of enriching gamma-aminobutyric - Google Patents
The highland barley germination technology of enriching gamma-aminobutyric Download PDFInfo
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- CN108740783A CN108740783A CN201810588944.3A CN201810588944A CN108740783A CN 108740783 A CN108740783 A CN 108740783A CN 201810588944 A CN201810588944 A CN 201810588944A CN 108740783 A CN108740783 A CN 108740783A
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- highland barley
- aminobutyric
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- seed
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- 235000007340 Hordeum vulgare Nutrition 0.000 title claims abstract description 121
- 230000035784 germination Effects 0.000 title claims abstract description 68
- 238000005516 engineering process Methods 0.000 title claims abstract description 33
- 240000005979 Hordeum vulgare Species 0.000 title 1
- 241000209219 Hordeum Species 0.000 claims abstract description 120
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 claims abstract description 59
- 229960003692 gamma aminobutyric acid Drugs 0.000 claims abstract description 30
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 16
- 238000007654 immersion Methods 0.000 claims abstract description 11
- 239000012153 distilled water Substances 0.000 claims abstract description 8
- 235000019441 ethanol Nutrition 0.000 claims abstract description 8
- 238000004140 cleaning Methods 0.000 claims abstract description 7
- 238000011282 treatment Methods 0.000 claims abstract description 5
- 238000012360 testing method Methods 0.000 claims description 10
- 238000011534 incubation Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 abstract description 8
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 238000012549 training Methods 0.000 description 4
- QWCKQJZIFLGMSD-UHFFFAOYSA-N 2-Aminobutanoic acid Natural products CCC(N)C(O)=O QWCKQJZIFLGMSD-UHFFFAOYSA-N 0.000 description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- QWCKQJZIFLGMSD-GSVOUGTGSA-N D-alpha-aminobutyric acid Chemical compound CC[C@@H](N)C(O)=O QWCKQJZIFLGMSD-GSVOUGTGSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 102100021943 C-C motif chemokine 2 Human genes 0.000 description 1
- 101000897480 Homo sapiens C-C motif chemokine 2 Proteins 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 241001300479 Macroptilium Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 229940124277 aminobutyric acid Drugs 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 230000002082 anti-convulsion Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- HQVFCQRVQFYGRJ-UHFFFAOYSA-N formic acid;hydrate Chemical compound O.OC=O HQVFCQRVQFYGRJ-UHFFFAOYSA-N 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 230000000147 hypnotic effect Effects 0.000 description 1
- 208000018875 hypoxemia Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000012209 synthetic fiber Substances 0.000 description 1
- 229920002994 synthetic fiber Polymers 0.000 description 1
- 238000004704 ultra performance liquid chromatography Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/152—Cereal germ products
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention provides a kind of highland barley germination technologies of enriching gamma-aminobutyric, include the following steps：Step 1: being sterilized to highland barley seed：Selected full, unabroken highland barley seed is then clean wash with distilled water with 60~80% ethyl alcohol 4~6min of immersion treatment；Step 2: impregnating germination：Highland barley seed after cleaning up impregnates 5~8h at ambient temperature, it is uniformly layered in a culture basket after pulling out, is placed in temperature and maintains in 35~37 DEG C of germination apparatus and carries out germination culture；Step 3: obtaining the highland barley seed that radical bud length is 7~8mm, the content of γ-aminobutyric acid is made to reach maximum.In this way, the content of γ-aminobutyric acid can be made to be increased to original 7 times, and this method step is simple, it is easy to operate.
Description
Technical field
The invention belongs to deep-processing technical field of agricultural products, more particularly to the highland barley of a kind of enriching gamma-aminobutyric germinates
Technique.
Background technology
γ-aminobutyric acid (Gamma Aminobutyric Acid, abbreviation GABA) is a kind of naturally occurring nonprotein
Amino acid is distributed widely in animal and plant body.All contain in plant such as Macroptilium, ginseng category, the seed of Chinese herbal medicine, rhizome and tissue fluid
There is GABA.γ-aminobutyric acid belongs to strong neural inhibitory aminoacid, has calmness, hypnosis, anticonvulsion, blood pressure lowering physiological action.
Highland barley is the first grande culture object of China Tibetan area, is the main grain ration of Tibetan compatriot.However, highland barley is by scientific research people
The concern and favor of member and consumers in general, more from it with health value outstanding.Highland barley rich in γ-aminobutyric acid,
The plurality of health care functions component such as flavonoids.Since γ-aminobutyric acid plays people's health important positive effect, improve
Alpha-aminobutyric acid content in animal and plant body is especially important.The structure of cereal can be changed in germination, adds as a kind of traditional
Work means are used in field of food.In the prior art, it is generally impregnated using hypoxemia, enrichment γ-is made in the means for coercing germination
The fructus hordei germinatus of aminobutyric acid.But fructus hordei germinatus can make the content of γ-aminobutyric acid reach best under what circumstances, be all not have
It refers to.
Invention content
For overcome the deficiencies in the prior art, the present invention provides a kind of highland barley germination technologies of enriching gamma-aminobutyric
Method, in this way, the content of γ-aminobutyric acid can be made to be increased to original 7 times, and this method step is simple, easily grasps
Make.
In order to realize these purposes and other advantages according to the present invention, the present invention provides a kind of enrichment gamma-amino fourths
The highland barley germination technology of acid, includes the following steps：
Step 1: being sterilized to highland barley seed：Selected full, unabroken highland barley seed, at 60~80% ethyl alcohol immersions
4~6min is managed, it is then clean wash with distilled water；
Step 2: impregnating germination：Highland barley seed after cleaning up impregnates 5~8h at ambient temperature, will after pulling out
It is uniformly layered in a culture basket, is placed in temperature and maintains in 35~37 DEG C of germination apparatus and carries out germination culture；
Step 3: obtaining the highland barley seed that radical bud length is 7~8mm, the content of γ-aminobutyric acid is made to reach maximum.
Preferably, the highland barley germination technology of the enriching gamma-aminobutyric in the step 2, is germinateing
In incubation, highland barley seed is cleaned once with warm water every 6h.
Preferably, the highland barley germination technology of the enriching gamma-aminobutyric, the step 3 further include later：It will
The highland barley seed of 7~8mm of radical bud length is placed in 50~58 DEG C of air dry oven and dries to constant weight.
Preferably, the highland barley germination technology of the enriching gamma-aminobutyric, the kind of selected highland barley seed are to hide
Blueness 320.
Preferably, the highland barley germination technology of the enriching gamma-aminobutyric, the culture basket used in the step 2
It is provided with multilayer, every layer of culture apparatus for being respectively provided with a culture highland barley seed, the culture apparatus places highland barley including one
The silk screen of seed is arranged four supporting rods for supporting the silk screen on four angles of the silk screen, and is arranged under the silk screen
The plate body of side, it is 7~8mm to be placed on distance L of the highland barley seed on the silk screen apart from the plate body.
Preferably, every layer of the highland barley germination technology of the enriching gamma-aminobutyric, the culture basket is respectively provided with one
Infrared sensor, the level height for the infrared ray that the infrared sensor is sent out is equal with the upper level of the laminate body, uses
Whether the radical bud to detect highland barley seed touches the laminate body, and every layer of infrared sensor is connected with a weak signal testing controller
It connects, and the data measured is transmitted to the weak signal testing controller, the weak signal testing controller is connected with an alarm.
Preferably, the highland barley germination technology of the enriching gamma-aminobutyric, the silk screen of described culture every layer of silk screen of basket
Aperture is less than the diameter of highland barley seed.
Preferably, the highland barley germination technology of the enriching gamma-aminobutyric, every layer of silk screen of the culture basket is with can
Mobile mode is arranged, and specific set-up mode is:The bottom end of each supporting rod is respectively provided with an idler wheel, easy to remove.
Preferably, the highland barley germination technology of the enriching gamma-aminobutyric, each supporting rod include a spiral shell
Bar and the nut being sleeved on the screw rod, rotate the height that the nut adjusts the supporting rod.
Preferably, the highland barley germination technology of the enriching gamma-aminobutyric, the culture basket are provided with 3~5 layers.
The present invention includes at least following advantageous effect：Pass through experimental study, after highland barley germination, the content of γ-aminobutyric acid
It can dramatically increase.But radical bud length is different, the content of γ-aminobutyric acid is also different, with the increase of radical bud length, γ-ammonia
The content of base butyric acid can also increase, and reach peak when radical bud length is 7~8mm, radical bud length is more than γ-ammonia after 7~8mm
The content of base butyric acid will decline, and obtain the highland barley seed that radical bud length is 7~8mm, the content of γ-aminobutyric acid can be made to reach
To maximum.The structure of culture basket of the present invention, culture basket are provided with multilayer, every layer of culture dress for being respectively provided with a culture highland barley seed
It sets, the culture apparatus includes one to place the silk screen of highland barley seed, and setting supports the silk screen on four angles of the silk screen
Four supporting rods, and the plate body below the silk screen is set, is placed on described in the highland barley seed distance on the silk screen
The distance L of plate body is 7~8mm, when the radical bud length of highland barley seed is more than or is 7~8mm, will touch plate body, energy
The enough radical bud length that easily obtains is the highland barley seed in a preset range, simple in structure, easy to operation.
Part is illustrated to embody by further advantage, target and the feature of the present invention by following, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Description of the drawings
Fig. 1 is the highland barley germination technology flow diagram of enriching gamma-aminobutyric in the embodiment of the present invention；
Fig. 2 is the change curve for the content that experimentation is directed to the γ-aminobutyric acid that radical bud length difference measures；
Fig. 3 is the structural schematic diagram that basket is cultivated in the embodiment of the present invention；
Fig. 4 is the structural schematic diagram of supporting rod in culture basket in the embodiment of the present invention；
Fig. 5 is the top cross-sectional view that basket is cultivated in the embodiment of the present invention.
Specific implementation mode
Present invention will be described in further detail below with reference to the accompanying drawings, to enable those skilled in the art with reference to specification text
Word can be implemented according to this.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or more
The presence or addition of a other elements or combinations thereof.
Shown in Fig. 1~3, the highland barley germination technology of enriching gamma-aminobutyric provided in an embodiment of the present invention, including following step
Suddenly：
Step 1: being sterilized to highland barley seed：Selected full, unabroken highland barley seed, at 60~80% ethyl alcohol immersions
4~6min is managed, it is then clean wash with distilled water.
Wherein, the kind of the selected highland barley seed of the embodiment of the present invention is dark blue 320.It is selected when selected highland barley seed ripe
Full, not damaged seed completely after being sterilized using ethyl alcohol, can be cleaned with distilled water and repeatedly ensure that highland barley wash seeds are dry
Only.
Step 2: impregnating germination：Highland barley seed after cleaning up impregnates 5~8h at ambient temperature, will after pulling out
It is uniformly layered in a culture basket, is placed in temperature and maintains in 35~37 DEG C of germination apparatus and carries out germination culture.
When impregnating seed, is impregnated using warm water, by disinfection, the foraminate training in bottom is spread it evenly over after immersion
It supports in basket.In carrying out germination incubation, highland barley seed is cleaned once with warm water every 6h.
Step 3: obtaining the highland barley seed that radical bud length is 7~8mm, the content of γ-aminobutyric acid is made to reach maximum.
It is dried to perseverance Step 4: the highland barley seed of 7~8mm of radical bud length is placed in 50~58 DEG C of air dry oven
Weight.
By experimental study, after highland barley germination, the content of γ-aminobutyric acid can dramatically increase.But radical bud length is not
Together, the content of γ-aminobutyric acid is also different, and with the increase of radical bud length, the content of γ-aminobutyric acid can also increase, and work as root
Bud length reaches peak when being 7~8mm, and after radical bud length is more than 7~8mm, the content of γ-aminobutyric acid will decline, acquisition
Radical bud length is the highland barley seed of 7~8mm, and the content of γ-aminobutyric acid can be made to reach maximum.
Specific experimentation is as follows, and highland barley germination is very fast, is generally completed in 96h, and speed of germination is uneven, therefore with blueness
Highland barley germinates (radical bud) length for variable.Respectively to different radical bud length (1-2mm, 4-5mm, 7-8mm, 11-12mm, 14-15mm,
17-18mm, 21-22mm, 25mm or more) highland barley sampling (rounding highland barley), and sample is placed in 55 DEG C of air dry ovens
Drying to constant weight, cooling is ground into powder with pulverizer, crosses 60 mesh sieve, saves backup.
The measurement of γ-aminobutyric acid：The content of γ-aminobutyric acid is measured using UPLC methods, with γ-aminobutyric acid standard items
For control.Analyte derivative：By 1mL sample extracting solutions, the borate buffer of pH value=8 0.6mL and the methanol HN of 1mL 0.3%
Solution mixes, and heats 10min under the conditions of 80 DEG C, is rapidly cooled to room temperature.Chromatographic condition：1 μ L of sample size, 35 DEG C of column temperature, stream
Fast 0.3mLmin-1, Detection wavelength 330nm；Mobile phase A：0.1% formic acid water, Mobile phase B：Acetonitrile.Condition of gradient elution：0
~4min20%B, 4min 40%B, 5~6min 100%B, 6~10min 20%B.
From attached drawing 2, we can clearly obtain the variation with radical bud length, the variation of alpha-aminobutyric acid content,
Reach peak when radical bud length is 7~8mm, that is to say, that alpha-aminobutyric acid content reaches maximum.
In a wherein specific implementation mode, as shown in Figure 3 and Figure 5, the highland barley of the enriching gamma-aminobutyric germinates
Technique, the culture basket 1 used in the step 2 are provided with multilayer, every layer of culture apparatus for being respectively provided with a culture highland barley seed,
The culture apparatus includes one to place the silk screen 2 of highland barley seed, is arranged and supports the silk screen on 2 four angles of the silk screen
Four supporting rods 3, and the plate body 4 that is arranged below the silk screen are placed on described in the highland barley seed distance on the silk screen
The distance L of plate body is 7~8mm.When the radical bud length of highland barley seed is more than or is 7~8mm, plate body will be touched, is propped up
It is the highland barley seed in a preset range that the setting of strut and plate body, which can easily obtain radical bud length, simple in structure, is convenient for
Operation.Since the speed of every germination of highland barley seed is different, faster or slower certainly can first reach radical bud length and want
The taking-up asked, it is remaining to continue to cultivate.After highland barley seed is paved on silk screen, gauze can also be covered, makes germination effect more
It is good.Material about silk screen can select synthetic fibers.
In a wherein specific implementation mode, the highland barley germination technology of the enriching gamma-aminobutyric, the culture basket
Every layer of 1 is respectively provided with an infrared sensor 7, the level height for the infrared ray that the infrared sensor 7 is sent out and the laminate body
Upper level is equal, and whether the radical bud for detecting highland barley seed touches the laminate body, and every layer of infrared sensor is with one
Weak signal testing controller 6 is connected, and the data measured are transmitted to the weak signal testing controller, the weak signal testing controller with
One alarm (being not drawn into figure) is connected.Infrared sensor and weak signal testing controller can be fixed on culture basket, work as alarm
When sounding, determine that the radical bud of highland barley seed touches plate body, the radical bud of some seed has reached requirement certainly.It is miniature
8 HC11 microcontroller PLC68 of MCHC11F1CFNE4 may be used in PLC controls, can also use the miniature control of other models
Device processed, as long as the function of the present embodiment can be completed.
In a wherein specific implementation mode, the highland barley germination technology of the enriching gamma-aminobutyric, the culture basket
The screen diameter of every layer of silk screen is less than the diameter of highland barley seed.The aperture of silk screen need to ensure the short diameter less than highland barley seed, protect
Highland barley seed on card silk screen cannot fall down from silk screen hole, meanwhile, it also allows for taking the highland barley seed of germination from silk screen
Go out.
In a wherein specific implementation mode, the highland barley germination technology of the enriching gamma-aminobutyric, the culture basket
Every layer of silk screen is arranged in a movable manner, and specific set-up mode is:The bottom end of each supporting rod is respectively provided with an idler wheel
5, it is easy to remove.Silk screen is arranged in a movable manner, convenient for taking out silk screen from culture basket, further also allows for silk screen
On highland barley seed be rinsed.
In a wherein specific implementation mode, as shown in figure 4, the highland barley germination technology of the enriching gamma-aminobutyric,
The nut 8 that each supporting rod screw rod 9 and be sleeved on the screw rod including one, rotates the nut and adjusts the supporting rod
Height.
When it is implemented, highland barley seed uniformly paves to silk screen, the height of supporting rod can be adjusted with rotating nuts, to
Distance of the highland barley seed apart from plate body is adjusted, accuracy rate is improved.
In a wherein specific implementation mode, the highland barley germination technology of the enriching gamma-aminobutyric, the culture basket
It is provided with 3~5 layers.The number of culture basket can be specifically arranged according to actual conditions, and the embodiment of the present invention does not do specific limit
Fixed, culture basket is preferably 3 layers.
Specific embodiment is given below to illustrate the present invention.
Embodiment 1
Step 1: being sterilized to highland barley seed：Selected full, unabroken highland barley seed, with 60% ethyl alcohol immersion treatment
6min is then clean wash with distilled water.
Step 2: impregnating germination：Highland barley seed after cleaning up impregnates 5h at ambient temperature, after pulling out by it
It is even to be layered in a culture basket, it is placed in temperature and maintains in 36 DEG C of germination apparatus and carry out germination culture.
When impregnating seed, is impregnated using warm water, by disinfection, the foraminate training in bottom is spread it evenly over after immersion
It supports in basket.In carrying out germination incubation, highland barley seed is cleaned once with warm water every 6h.
Step 3: obtaining the highland barley seed that radical bud length is 7~8mm, the content of γ-aminobutyric acid is made to reach maximum.
It is dried to constant weight Step 4: the highland barley seed of 7~8mm of radical bud length is placed in 50 DEG C of air dry oven.
Embodiment 2
Step 1: being sterilized to highland barley seed：Selected full, unabroken highland barley seed, with 70% ethyl alcohol immersion treatment
5min is then clean wash with distilled water.
Step 2: impregnating germination：Highland barley seed after cleaning up impregnates 5h at ambient temperature, after pulling out by it
It is even to be layered in a culture basket, it is placed in temperature and maintains in 37 DEG C of germination apparatus and carry out germination culture.
When impregnating seed, is impregnated using warm water, by disinfection, the foraminate training in bottom is spread it evenly over after immersion
It supports in basket.In carrying out germination incubation, highland barley seed is cleaned once with warm water every 6h.
Step 3: obtaining the highland barley seed that radical bud length is 7~8mm, the content of γ-aminobutyric acid is made to reach maximum.
It is dried to constant weight Step 4: the highland barley seed of 7~8mm of radical bud length is placed in 55 DEG C of air dry oven.
Embodiment 3
Step 1: being sterilized to highland barley seed：Selected full, unabroken highland barley seed, with 80% ethyl alcohol immersion treatment
4min is then clean wash with distilled water.
Step 2: impregnating germination：Highland barley seed after cleaning up impregnates 8h at ambient temperature, after pulling out by it
It is even to be layered in a culture basket, it is placed in temperature and maintains in 35 DEG C of germination apparatus and carry out germination culture.
When impregnating seed, is impregnated using warm water, by disinfection, the foraminate training in bottom is spread it evenly over after immersion
It supports in basket.In carrying out germination incubation, highland barley seed is cleaned once with warm water every 6h.Germination apparatus is that constant temperature is automatic
Sprouting machine.
Step 3: obtaining the highland barley seed that radical bud length is 7~8mm, the content of γ-aminobutyric acid is made to reach maximum.
It is dried to constant weight Step 4: the highland barley seed of 7~8mm of radical bud length is placed in 58 DEG C of air dry oven
As described above, the highland barley germination technology method of enriching gamma-aminobutyric provided by the invention, in this way, energy
The content of γ-aminobutyric acid is enough set to be increased to original 7 times, and this method step is simple, it is easy to operate.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With.It can be applied to various suitable the field of the invention completely.It for those skilled in the art, can be easily
Realize other modification.Therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and legend shown and described herein.
Claims (10)
1. the highland barley germination technology of enriching gamma-aminobutyric, which is characterized in that include the following steps：
Step 1: being sterilized to highland barley seed：Selected full, unabroken highland barley seed, with 60~80% ethyl alcohol immersion treatments 4~
6min is then clean wash with distilled water；
Step 2: impregnating germination：Highland barley seed after cleaning up impregnates 5~8h at ambient temperature, after pulling out by it
It is even to be layered in a culture basket, it is placed in temperature and maintains in 35~37 DEG C of germination apparatus and carry out germination culture；
Step 3: obtaining the highland barley seed that radical bud length is 7~8mm, the content of γ-aminobutyric acid is made to reach maximum.
2. the highland barley germination technology of enriching gamma-aminobutyric as described in claim 1, which is characterized in that in the step 2,
In carrying out germination incubation, highland barley seed is cleaned once with warm water every 6h.
3. the highland barley germination technology of enriching gamma-aminobutyric as described in claim 1, which is characterized in that the step 3 it
After further include：The highland barley seed of 7~8mm of radical bud length is placed in 50~58 DEG C of air dry oven and is dried to constant weight.
4. the highland barley germination technology of the enriching gamma-aminobutyric as described in claims 1 to 3 is any, which is characterized in that selected
The kind of highland barley seed is dark blue 320.
5. the highland barley germination technology of enriching gamma-aminobutyric as described in claim 1, which is characterized in that in the step 2
The culture basket used is provided with multilayer, every layer of culture apparatus for being respectively provided with a culture highland barley seed, and the culture apparatus includes one
To place the silk screen of highland barley seed, four supporting rods that the silk screen is supported on four angles of the silk screen, and setting are set
Plate body below the silk screen, it is 7~8mm to be placed on distance L of the highland barley seed on the silk screen apart from the plate body.
6. the highland barley germination technology of enriching gamma-aminobutyric as claimed in claim 5, which is characterized in that the culture basket
Every layer is respectively provided with an infrared sensor, the upper surface of the level height for the infrared ray that the infrared sensor is sent out and the laminate body
Highly equal, whether the radical bud for detecting highland barley seed touches the laminate body, every layer of infrared sensor with a weak signal testing
Controller is connected, and the data measured are transmitted to the weak signal testing controller, the weak signal testing controller and an alarm
It is connected.
7. the highland barley germination technology of enriching gamma-aminobutyric as claimed in claim 5, which is characterized in that the culture basket is every
The screen diameter of layer silk screen is less than the diameter of highland barley seed.
8. the highland barley germination technology of enriching gamma-aminobutyric as claimed in claim 5, which is characterized in that the culture basket is every
Layer silk screen is arranged in a movable manner, and specific set-up mode is:The bottom end of each supporting rod is respectively provided with an idler wheel, just
In movement.
9. the highland barley germination technology of enriching gamma-aminobutyric as claimed in claim 8, which is characterized in that each support
Bar including a screw rod and the nut being sleeved on the screw rod, rotates the height that the nut adjusts the supporting rod.
10. the highland barley germination technology of enriching gamma-aminobutyric as claimed in claim 5, which is characterized in that the culture basket is set
It is equipped with 3~5 layers.
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| CN111109527A (en) * | 2020-01-20 | 2020-05-08 | 青海大学 | Highland barley germinated rice with high gamma-aminobutyric acid content, processing method and application |
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