CN108740332A - A kind of folium eucalypti polyphenol feed addictive and preparation method thereof improving meat quality - Google Patents
A kind of folium eucalypti polyphenol feed addictive and preparation method thereof improving meat quality Download PDFInfo
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- CN108740332A CN108740332A CN201810531934.6A CN201810531934A CN108740332A CN 108740332 A CN108740332 A CN 108740332A CN 201810531934 A CN201810531934 A CN 201810531934A CN 108740332 A CN108740332 A CN 108740332A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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Abstract
The present invention provides a kind of folium eucalypti polyphenol feed addictive and preparation method thereof improving meat quality, for the higher folium eucalypti polyphenol of purity prepared from eucalyptus leaves, preparation method is to crush eucalyptus leaf raw material by cryogenic freezing, it is fitted into continuous phase transistion extraction kettle and seals extraction, collect eucalyptus leaf extract, it is concentrated under reduced pressure, centrifugal filtration obtains eucalyptus leaves Aqueous extracts, then vacuum freeze drying obtains eucalyptus Leave extract, column chromatography for separation is carried out again, it is that eluant, eluent carries out gradient elution to select ethyl alcohol and ethyl acetate, collect the eluent containing folium eucalypti polyphenol, eluent is concentrated in vacuo, it is subsequently placed in and is dried in vacuo up to folium eucalypti polyphenol.Oenothera biennis element B and 1 of the present invention, 2,3,4,6- five galloyl glucoses, the recovery rate and purity of the folium eucalyptis polyphenol effective active component such as English oak (Quercus robur) tan florigen and 5- methoxyl group furfurals are high, can be applied to the natural plant feed addictive for improving meat duck meat.
Description
Technical field
The present invention relates to a kind of natural plant feed additives, more particularly to a kind of folium eucalypti polyphenol improving meat quality
Feed addictive, preparation method and application.
Background technology
With the development of feed additive industry, the chemical syntheses such as antibiotic bring a series of harm as feed adds
The development of agent industry, the chemical syntheses such as antibiotic bring a series of harm to have become common recognition, such as drug resistance, object residual and teratogenesis
At common recognition.Now, in order to improve meat quality, also more next high researcher is dedicated to developing new type natural and adds for requirement to feed
The demand for adding agent to seek to fill that people produce high-quality herding.Chinese herbal feed additive seeks to fill that people to high-quality herding
The demand of production.Chinese herbal feed additive improves meat significant effect etc. because of its noresidue in Modern Animal Husbandry and with very strong
Feature gradually has been favored by people.The grinds such as Chinese herbal medicine are either compounded and is added to the daily ration of animal or drinks
In water, it is intended to it improves the works such as anti-stress ability and the feed interest rate of animal and then improves a kind of preparation of animal meat quality product, this
Kind preparation is referred to as Chinese herbal feed additive.
With the development of economy and society, high-quality meat products is gradually favored by people, it is not only safe, full of nutrition, and
Also there are relatively good taste and sensory properties, therefore select natural, safe Chinese herbal medicine extract to have improved livestock and poultry meat
As current research hot spot.Animal husbandry is always the mainstay production of China's agriculture, and the big demand in market is also big, therefore wants to pass through medium-height grass
Medicament extract just has to look for the very abundant middle grass resource of resource to improve livestock and poultry meat.Eucalyptus is resourceful in China, tool
There is the features such as area is big, region is extensively wide in variety.The research applied in animal feed industries about folium eucalypti extract at present is very
It is few.Some researches show that folium eucalypti extracts to be applied to broiler chicken, egg and the daily feed of pig, is conducive to significantly improve body growth
Energy, anti-oxidant and meat index of correlation.Also experiment shows that folium eucalypti polyphenol extract all has very strong antioxygen in vivo and outside
Property activity, but less researcher by folium eucalypti polyphenol be applied to duck feed addictive.
It is " a kind of for improving plant extracts additive of meat and preparation method thereof and answering disclosed in Chinese patent application
With "（CN201710886845.9）, it is prepared for a kind of including folium eucalypti extract, purple common perilla seed, eucommia ulmoides extracts and yeast
The compounding ingredients such as extract, for improving the plant extracts additive of meat in pannage, poultry feed or fish meal
Using, be not directed to folium eucalypti polyphenol be applied to duck feed addictive.
" a kind of eucalyptus leaf extract natural meat quality improver " disclosed in Chinese patent application（CN201310071057.6）,
A kind of folium eucalypti extract natural meat quality improver, preparation method and purposes are provided, is to be protected from light eucalyptus leaves to dry in the shade, is crushed to 40-
60 mesh are added in extractor, and the extraction of 40-95% ethyl alcohol is added twice, enrichment facility is added after merging filtrate, are concentrated into only remaining
Water is spray-dried.Total phenol content of the present invention is 25 ~ 40%, containing oenothera biennis element B6 ~ 12%, trie horse element I 1 ~ 2%, 1,2,
3,4,6 Penta-O-galloyl-D-glucopyranoses 0.1 ~ 0.15%.The patent is by eucalyptus leaf extract additive for farm animal feed, and wherein folium eucalypti
Polyphenol content is relatively low, is not directed to folium eucalypti polyphenol purifying process, and folium eucalypti polyphenol is still required study applied to duck feed addictive.
Invention content
The purpose of the present invention is to provide a kind of folium eucalypti polyphenol feed addictives of meat quality, pass through distinctive preparation
Method is extracted to obtain the folium eucalypti extract of folium eucalypti polyphenol high-content, is used for duck feed addictive, can improve intramuscular fat and fat
Acid composition, improves the content of the amino acid such as guanylic acid, histidine, glycine, to improve duck meat quality.
The purpose of the present invention is to provide one kind preparing the higher folium eucalypti polyphenol of purity from eucalyptus leaves, by including the following steps
Method be made：
（1）Eucalyptus leaf raw material is crushed by cryogenic freezing, is fitted into continuous phase transistion extraction kettle and seals extraction, eucalyptus leaves is collected and carries
It takes.
（2）Eucalyptus leaf extract is concentrated under reduced pressure, centrifugal filtration obtains eucalyptus leaves Aqueous extracts, and then vacuum freeze drying obtains
To eucalyptus Leave extract.
（3）Eucalyptus Leave extract carries out column chromatography for separation, and it is that eluant, eluent carries out gradient elution to select ethyl alcohol and ethyl acetate,
Folin-Phenol colorimetric method monitors, and collects the eluent containing folium eucalypti polyphenol.
（4）Eluent is concentrated in vacuo, is subsequently placed in and is dried in vacuo up to folium eucalypti polyphenol.
As an optimization, step（1）In, the eucalyptus leaves adopt the 9-10 months " wide woods No. 9 " eucalyptus leaves in Zhanjiang, are crushed to
60-80 mesh.
As an optimization, step（1）In, the extracting pressure of the continuous phase transistion extraction is 1~1.5 MPa, and extraction temperature is
50~75 DEG C, resolution temperature is 50~75 DEG C, and extraction time is 50~100min, using 60-90% ethyl alcohol as extractant.
As an optimization, step（2）In, when the centrifugal filtration, centrifugal speed is 4000~6000rpm, centrifugation 10~
30min。
As an optimization, step（3）In, the column chromatography for separation use silica gel or macroreticular resin, silica gel select 100~
300 mesh silica gel, macroreticular resin select polyamide, and the flow velocity of elution is 40~60mL/min, and ladder is carried out with ethyl alcohol and ethyl acetate
The volume proportion of degree elution, ethyl alcohol and ethyl acetate is followed successively by 100:0,85:15,65:35,55:45,45:55,35:65,15:
85,5:95, it is concentrated in vacuo ethyl alcohol and ethyl acetate volume ratio 65:35,45:55 eluent, as folium eucalypti polyphenol.
It is 80~85% that folium eucalypti polyphenol produced by the present invention measures folium eucalypti polyphenol content through Folin-Phenol colorimetrically analysing, and profit
It is measured with efficient liquid phase chromatographic analysis and finds that it contains a large amount of oenothera biennis element B, 1,2,3,4,6- five galloyl grape
Sugar, English oak (Quercus robur) tan florigen, the ingredients such as 5- methoxyl group furfurals, wherein oenothera biennis element B content 55~65%.
Folium eucalypti polyphenol provided by the invention as additive amount of the natural plant additive in duck feed be 0.02~
0.15%。
The present invention has the positive effect that：
（1）Eucalyptus leaves make the content of folium eucalypti polyphenol in eucalyptus leaves be improved after continuous phase transistion extracts, a large amount of to collect favorably
In the extraction and purifying in later stage.
（2）Folium eucalypti polyphenol proposed by the present invention extracts, refines new process route:Low temperature continuous phase transistion extraction-column chromatography
Separation-crystal refining, relative to solvent extraction, step is obviously simple, high income.Relative to low temperature continuous phase transistion extraction
Method, increases column chromatography for separation process, and the yield of folium eucalypti polyphenol is but greatly improved.The extraction of folium eucalypti polyphenol, refined work
For the total recovery of skill process 50% or more, purity reaches 80% or more.Therefore, which has stronger prospects for commercial application.
（3）Folium eucalypti polyphenol recovery rate and content of the present invention are high, and purity is higher, and active ingredient is clear.
（4）Folium eucalypti polyphenol has good inoxidizability and anti-stress ability, can improve meat as duck feed addictive
Color improves intramuscular fat and aliphatic acid composition, the content of the amino acid such as guanylic acid, histidine, glycine is improved, to improve duck
Meat meat quality.
Specific implementation mode
Below in conjunction with actual conditions, the specific implementation mode of the present invention is described in detail.
Embodiment 1：
Eucalyptus leaf raw material is crushed by cryogenic freezing, 40 mesh is crushed to, weighs and crush raw material 10kg, is packed into continuous phase transistion extraction
Extraction is sealed in kettle, extracting pressure is 1 MPa, and extraction temperature is 70 DEG C, and resolution temperature is 75 DEG C, extraction time 100min,
Using 60% ethyl alcohol as extractant, eucalyptus leaf extract is collected.Eucalyptus leaf extract is concentrated under reduced pressure, eucalyptus leaves water is obtained by filtration and carries
Liquid, then vacuum freeze drying obtain eucalyptus Leave extract.Eucalyptus Leave extract crosses 200 mesh silicagel columns, and the flow velocity of elution is
40ml/min carries out gradient elution with ethyl alcohol and ethyl acetate, and the volume proportion of ethyl alcohol and ethyl acetate is followed successively by 100:0,85:
15,65:35,55:45,45:55,35:65,15:85,5:95, it is concentrated in vacuo ethyl alcohol and ethyl acetate volume ratio 65:35,45:55
Eluent, as folium eucalypti polyphenol.Eluent is concentrated in vacuo, vacuum freeze drying is up to folium eucalypti polyphenol 511.9g, recovery rate
It is 56%, liquid phase detection contains 60% oenothera biennis element B.Additive amount of the folium eucalypti polyphenol as natural plant additive in duck feed
It is 0.02~0.15%.
Embodiment 2：
Eucalyptus leaf raw material is crushed by cryogenic freezing, 60 mesh is crushed to, weighs and crush raw material 20kg, is packed into continuous phase transistion extraction
Extraction is sealed in kettle, extracting pressure is 0.8 MPa, and extraction temperature is 60 DEG C, and resolution temperature is 65 DEG C, and extraction time is
100min collects eucalyptus leaf extract using 80% ethyl alcohol as extractant.Eucalyptus leaf extract is concentrated under reduced pressure, eucalyptus is obtained by filtration
Leaf Aqueous extracts, then vacuum freeze drying obtain eucalyptus Leave extract.Eucalyptus Leave extract crosses 200 mesh silicagel columns, the stream of elution
Speed is 30mL/min, carries out gradient elution with ethyl alcohol and ethyl acetate, the volume proportion of ethyl alcohol and ethyl acetate is followed successively by 100:
0,85:15,65:35,55:45,45:55,35:65,15:85,5:95, it is concentrated in vacuo ethyl alcohol and ethyl acetate volume ratio 65:35,
45:55 eluent, as folium eucalypti polyphenol.Eluent is concentrated in vacuo, vacuum freeze drying up to folium eucalypti polyphenol 1015.1g,
Recovery rate is 50%, and liquid phase detection contains 58% oenothera biennis element B.Folium eucalypti polyphenol adding in duck feed as natural plant additive
Dosage is 0.02~0.15%.
Embodiment 3：
Eucalyptus leaf raw material is crushed by cryogenic freezing, 55 mesh is crushed to, weighs and crush raw material 10kg, is packed into continuous phase transistion extraction
Extraction is sealed in kettle, extracting pressure is 0.8 MPa, and extraction temperature is 65 DEG C, and resolution temperature is 75 DEG C, extraction time 80min,
Using 90% ethyl alcohol as extractant, eucalyptus leaf extract is collected.Eucalyptus leaf extract is concentrated under reduced pressure, eucalyptus leaves water is obtained by filtration and carries
Liquid, then vacuum freeze drying obtain eucalyptus Leave extract.The excessive hole resin column of eucalyptus Leave extract, the flow velocity of elution are
30mL/min carries out gradient elution with ethyl alcohol and ethyl acetate, and the volume proportion of ethyl alcohol and ethyl acetate is followed successively by 100:0,85:
15,65:35,55:45,45:55,35:65,15:85,5:95, it is concentrated in vacuo ethyl alcohol and ethyl acetate volume ratio 65:35,45:55
Eluent, as folium eucalypti polyphenol.Eluent is concentrated in vacuo, vacuum freeze drying is up to folium eucalypti polyphenol 499.2g, recovery rate
It is 50%, liquid phase detection contains 57% oenothera biennis element B.Folium eucalypti polyphenol is as additive amount of the natural plant additive in duck feed
0.02~0.15%.
To illustrate, verifying the effect that the present invention improves meat quality, spy has made following feeding experiment.
324 20 age in days meat ducks are selected in experiment, are arranged 6 experimental groups, every group of 6 repetitions, and each repeatedly 9
Meat duck, it is continuous to feed 40 d, acquire chest muscle and leg flesh after slaughtering, analysis meat duck muscle antioxygenic property, yellowish pink and nutrition at
Grade index.
The result shows that：Compared with the control group, in terms of Antioxidant Indexes, folium eucalypti polyphenol can significantly improve paddy in Hu meat duck muscle
The sweet peptide peroxidase of Guang（GSH-PX）Vigor, while significantly reducing malonaldehyde value（MDA）Content；In terms of meat quality, eucalyptus
Leaf polyphenol can significantly improve muscle yellowish pink, improve intramuscular fat content in muscle.From table 1, table 2 as can be seen that with addition
Being continuously increased from 0.02%-0.08% is measured, Hu meat duck muscle Glutathione Peroxidase vigor is improved and improves meat
The performance of matter steps up.But it is also shown that after additive amount is more than 0.08%, above-mentioned effect slowly increases for present invention experiment
By force, it when additive amount is more than 0.15%, improves Hu meat duck muscle Glutathione Peroxidase vigor and improves the work of meat
Use unobvious.Consider manufacturing cost and application effect, folium eucalypti polyphenol adding in duck feed as natural plant additive
Dosage is 0.02~0.15%, and optimization is 0.08%.
Claims (10)
1. a kind of folium eucalypti polyphenol feed addictive improving meat quality, which is characterized in that by method comprising the following steps system
?：
（1）Eucalyptus leaf raw material is crushed by cryogenic freezing, is fitted into continuous phase transistion extraction kettle and seals extraction, eucalyptus leaves is collected and carries
Take liquid；
（2）Eucalyptus leaf extract is concentrated under reduced pressure, centrifugal filtration obtains eucalyptus leaves Aqueous extracts, and then vacuum freeze drying obtains eucalyptus
Leaf crude extract；
（3）Eucalyptus Leave extract carries out column chromatography for separation, and it is that eluant, eluent carries out gradient elution, good fortune to select ethyl alcohol and ethyl acetate
Woods-phenol colorimetric method monitoring, collects the eluent containing folium eucalypti polyphenol；
（4）Eluent is concentrated in vacuo, is subsequently placed in and is dried in vacuo up to folium eucalypti polyphenol.
2. a kind of folium eucalypti polyphenol feed addictive improving meat quality according to claim 1, which is characterized in that folium eucalypti
Polyphenol content be 80~85%, containing five galloyl glucose of oenothera biennis element B, 1,2,3,4,6-, English oak (Quercus robur) tan florigen and
5- methoxyl group furfurals, wherein oenothera biennis element B content 55~65%.
3. a kind of preparation method for the folium eucalypti polyphenol feed addictive improving meat quality, which is characterized in that include the following steps：
（1）Eucalyptus leaf raw material is crushed by cryogenic freezing, is fitted into continuous phase transistion extraction kettle and seals extraction, eucalyptus leaves is collected and carries
Take liquid；
（2）Eucalyptus leaf extract is concentrated under reduced pressure, centrifugal filtration obtains eucalyptus leaves Aqueous extracts, and then vacuum freeze drying obtains eucalyptus
Leaf crude extract；
（3）Eucalyptus Leave extract carries out column chromatography for separation, and it is that eluant, eluent carries out gradient elution, good fortune to select ethyl alcohol and ethyl acetate
Woods-phenol colorimetric method monitoring, collects the eluent containing folium eucalypti polyphenol；
（4）Eluent is concentrated in vacuo, is subsequently placed in and is dried in vacuo up to folium eucalypti polyphenol；
（5）Folium eucalypti polyphenol is 0.02~0.15% as additive amount of the natural plant additive in duck feed.
4. a kind of folium eucalypti polyphenol feed addictive preparation method improving meat quality according to claim 3, feature
It is, step（1）In, the eucalyptus leaves are adopted in the 9-10 months " wide woods No. 9 " eucalyptus leaves, and 60-80 mesh is crushed to.
5. the preparation method of the folium eucalypti polyphenol feed addictive according to claim 3 for improving meat quality, feature exist
In step（1）In, the extracting pressure of the continuous phase transistion extraction is 1~1.5 MPa, and extraction temperature is 50~75 DEG C, parsing temperature
Degree is 50~75 DEG C, and extraction time is 50~100min, using 60-90% ethyl alcohol as extractant.
6. the preparation method of the folium eucalypti polyphenol feed addictive according to claim 3 for improving meat quality, feature exist
In step（2）In, the centrifugal speed when centrifugal filtration centrifuges is 4000~6000rpm, centrifuges 10~30min.
7. the folium eucalypti polyphenol feed addictive preparation method according to claim 3 for improving meat quality, which is characterized in that
Step（3）In, the column chromatography for separation uses silica gel, silica gel to select 100~300 mesh silica gel.
8. the preparation method of the folium eucalypti polyphenol feed addictive according to claim 3 for improving meat quality, feature exist
In step（3）In, the column chromatography for separation uses macroreticular resin, macroreticular resin to select polyamide.
9. a kind of preparation side of the folium eucalypti polyphenol feed addictive of raising meat quality according to claim 3 or 7 or 8
Method, which is characterized in that step（3）In, the flow velocity of elution is 40~60mL/min, carries out gradient with ethyl alcohol and ethyl acetate and washes
De-, the volume proportion of ethyl alcohol and ethyl acetate is followed successively by 100:0,85:15,65:35,55:45,45:55,35:65,15:85,5:
95, it is concentrated in vacuo ethyl alcohol and ethyl acetate volume ratio 65:35,45:55 eluent.
10. a kind of application process of the folium eucalypti polyphenol feed addictive as described in claim 1 improving meat quality, feature
It is, the additive amount in duck feed is the 0.02~0.15% of feed relative.
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Cited By (1)
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| CN115400165A (en) * | 2022-08-15 | 2022-11-29 | 华南农业大学 | Eucalyptus leaf extract, preparation method and application thereof in preparation of weight-losing and lipid-lowering products |
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Application publication date: 20181106 |