CN108734620A - A kind of size of population investigation method based on concentration of wastewater monitoring - Google Patents
A kind of size of population investigation method based on concentration of wastewater monitoring Download PDFInfo
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- CN108734620A CN108734620A CN201810525954.2A CN201810525954A CN108734620A CN 108734620 A CN108734620 A CN 108734620A CN 201810525954 A CN201810525954 A CN 201810525954A CN 108734620 A CN108734620 A CN 108734620A
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- population
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06Q—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES; SYSTEMS OR METHODS SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES, NOT OTHERWISE PROVIDED FOR
- G06Q50/00—Systems or methods specially adapted for specific business sectors, e.g. utilities or tourism
- G06Q50/10—Services
- G06Q50/26—Government or public services
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
Abstract
The invention discloses a kind of size of population investigation methods based on concentration of wastewater monitoring, specifically include following steps：S1：The region for determining the pre- estimation size of population selects the sewage treatment plant in the region, acquisition sewage plant to be pre-processed into water sample and to sample；S2：Select body metabolism product ammonia nitrogen and the metabolite cotinine of tobacco as biomarker；S3：The ammonia nitrogen concentration in sewage is measured using National Standard Method, wherein national standard law regulation measures the ammonia nitrogen concentration in sewage using Berthelot spectrophotometry；S4：Cotinine concentration is measured using liquid chromatography mass instrument or gas chromatography-mass spectrometry；S5：The yield per capita for calculating two kinds of biomarkers calculates the size of population for obtaining the sewage treatment plant region by biological marker method.
Description
Technical field
The present invention relates to size of population technique for investigation field more particularly to a kind of sizes of population based on concentration of wastewater monitoring
Investigation method.
Background technology
Size of population investigation is national management, formulates population policy, labour employment policy, policy for education, social welfare political affairs
The important foundation such as plan.Currently, that be most widely used is census method.When census method refers to as defined in unification of the motherland
In, according to unified method, unified project, unified application form and unified standard time point, to national population generally,
The single-round survey and registration by people carried out from door to door.The accurate acquisition size of population of this method energy, can reflect permanent residence
The size of population of the people, but investigation economic cost is higher, spends the time longer, it is difficult to count floating population's quantity.In addition, the number
According to the size of population that can only reflect in one period, it cannot reflect the dynamic change of the short time size of population.
Another more universal method is to estimate population according to communication common carrier service is used in net activity mobile phone quantity
Quantity.Communication common carrier identification at present reaches in net mobile phone user exact position technology resolution ratio within 30m, is fully able to meet essence
Really calculate the requirement of the real-time size of population.By investigating communication common carrier activity mobile phone number of users, by technologies such as identifications
Means correction individual possesses two and the above mobile phone situation, can obtain the region real-time activity size of population.This method can be accurate
The size of population of true acquisition area that are fairly developed economically, can be provided by the real-time size of population of network's coverage area, but this tune
The assistance for needing mobile communication company is looked into, for not using the user of mobile phone, the investigation not comprehensive.
Invention content
According to problem of the existing technology, the invention discloses a kind of size of population investigation based on concentration of wastewater monitoring
Method specifically includes following steps：
S1：The region for determining the pre- estimation size of population selects the sewage treatment plant in the region, acquisition sewage plant water inlet
Sample simultaneously pre-processes sample；
S2：Select body metabolism product ammonia nitrogen and the metabolite cotinine of tobacco as biomarker；
S3：The ammonia nitrogen concentration in sewage is measured using National Standard Method, wherein national standard law regulation uses nessler reagent spectrophotometric
Method measures the ammonia nitrogen concentration in sewage；
S4：Cotinine concentration is measured using liquid chromatography mass instrument or gas chromatography-mass spectrometry；
S5：The yield per capita for calculating two kinds of biomarkers is calculated by biological marker method and obtains the sewage treatment plant
The size of population of region.
The yield per capita of the ammonia nitrogen obtains in the following way：
In formula, mNH4-NFor the yield per capita of ammonia nitrogen；mIFor the Q days interior ammonia nitrogen total releases by enumeration district (ED)；PITo adjust
Look into the resident population in area.
The data of yield per capita of the cotinine obtain in the following way：
mCotinine=NCigarette×DNicotine×YNicotine×xCotinine
In formula, mCotinineFor cotinine yield per capita；NCigaretteFor daily amount of smoking per capita；DNicotineFor tobacco
The content of middle nicotine；YNicotineIt is human body to the intake of nicotine；xCotinineThe ratio being metabolized after human body is sucked for nicotine
Rate.
The size of population of the sewage treatment plant region obtains in the following way：
In formula, P is sewage treatment plant's real time service size of population, and C is the concentration of biomarker, and F is the discharge of sewage, m
For the quantity for the marker that the yield per capita of biomarker is drained or generated for each person every day.
In S3 specifically in the following way：
S31：Configuration measures reagent needed for ammonia nitrogen concentration, including potassium sodium tartrate solution and nessler reagent solution；
S32：Formulate standard curve：In 6 10mL colorimetric cylinders, be separately added into 0.00mL, 1.00mL, 2.00mL,
3.00mL, 4.00mL and 5.00mL ammonia nitrogen standard working solution, corresponding to ammonia-nitrogen content be respectively 0.0 μ g, 10.0 μ g,
20.0 μ g, 30.0 μ g, 40.0 μ g and 50.0 μ g, add water to graticule.200 μ l potassium sodium tartrate solutions are added, shake up.It adds
400 μ l nessler reagents, shake up.After placing 10min, at wavelength 420nm, with 20mm cuvettes, reference is made with water, measures extinction
Degree.
Using the absorbance after blank correction as ordinate, with its corresponding ammonia-nitrogen content (μ g) for abscissa, Excel is used
Software on Drawing calibration curve；
S33：The water sample 0.2mL acquired from sewage plant directly is taken, 2mL is diluted to and is measured by the step identical as standard curve
Absorbance；
S34：The absorbance value for measuring sample is brought into ready-made standard curve, ammonia nitrogen concentration is calculated；
In S4 specifically in the following way：
S41：Water sample after the acquisition of filter sewage factory, removes the suspended particulate substance in sewage
S42：Use solid phase extraction tobacco metabolite cotinine：Solid-phase extraction column is eluted first；Then loading,
By sample by solid-phase extraction column, cotinine stays in the solid phase filler of solid-phase extraction column at this time；Solid-phase extraction column is eluted, it can
For being rather eluted out from extraction column；
S43：Sample containing cotinine after extraction is put into liquid chromatography mass instrument or gas chromatography-mass spectrometry point
Analysis, measures the concentration of cotinine
By adopting the above-described technical solution, a kind of size of population investigation based on concentration of wastewater monitoring provided by the invention
Method, this method investigate the size of population based on municipal wastewater analysis, due to biomarker be by body metabolism generate and
The substance excreted, biomarker can be the metabolites of exogenous material, can also be the object that body metabolism generates
Matter, therefore this method estimates urban population quantity according to biomarker method, is had more compared to traditional census method's this method
There are objectivity and authenticity, and it is very low to investigate operating cost.
Description of the drawings
In order to illustrate the technical solutions in the embodiments of the present application or in the prior art more clearly, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments described in application, for those of ordinary skill in the art, without creative efforts,
Other drawings may also be obtained based on these drawings.
Fig. 1 is the flow chart of the method for the present invention.
Specific implementation mode
To keep technical scheme of the present invention and advantage clearer, with reference to the attached drawing in the embodiment of the present invention, to this
Technical solution in inventive embodiments is clearly completely described：
A kind of size of population investigation method based on concentration of wastewater monitoring as shown in Figure 1, includes the following steps：S1：Really
The region of the fixed pre- estimation size of population, selects the sewage treatment plant in the region, acquires sewage plant into water sample；
S2：Select body metabolism product ammonia nitrogen and the metabolite cotinine of tobacco as biomarker；
S3：The ammonia nitrogen concentration in sewage is measured using National Standard Method, national standard law regulation is surveyed using Berthelot spectrophotometry
Determine the ammonia nitrogen concentration in sewage；
S31：Configuration measures reagent needed for ammonia nitrogen concentration, including potassium sodium tartrate solution and nessler reagent solution；
S32：Formulate standard curve：In 6 10mL colorimetric cylinders, be separately added into 0.00mL, 1.00mL, 2.00mL,
3.00mL, 4.00mL and 5.00mL ammonia nitrogen standard working solution, corresponding to ammonia-nitrogen content be respectively 0.0 μ g, 10.0 μ g,
20.0 μ g, 30.0 μ g, 40.0 μ g and 50.0 μ g, add water to graticule.200 μ l potassium sodium tartrate solutions are added, shake up.It adds
400 μ l nessler reagents, shake up.After placing 10min, at wavelength 420nm, with 20mm cuvettes, reference is made with water, measures extinction
Degree.
Using the absorbance after blank correction as ordinate, with its corresponding ammonia-nitrogen content (μ g) for abscissa, Excel is used
Software on Drawing calibration curve；
S33：The water sample 0.2mL acquired from sewage plant directly is taken, 2mL is diluted to and is measured by the step identical as standard curve
Absorbance；
S34：The absorbance value for measuring sample is brought into ready-made standard curve, ammonia nitrogen concentration is calculated；
S4：Cotinine concentration is measured using gas chromatography-mass spectrometry；
S41：Sample filtering：10ml sewage sample priority 0.45um, 0.22um glass fiber filters filter, to remove sample
Solid in product and suspended particulate.
S42：Solid-phase extraction column activates：10ml methanol, 10ml ultra-pure waters are used successively
S43：It rinses：With the ultrapure water wash solid-phase extraction columns of 6ml
S44：It is dry：0.8h-1.5h is persistently evacuated with vacuum pump makes solid-phase extraction column dry
S45：Elution：Solid-phase extraction column after using the elution of 6ml methanol dry successively
S46：Nitrogen is blown：Eluent is dried up with nitrogen stream is mitigated
S47：Solvent is replaced：It uses acetonitrile to be transferred in 2ml chromatogram bottles with rubber head dropper as solvent, is settled to 1ml
S48：Chromatogram bottle is put into gas chromatography-mass spectrometry and is analyzed, cotinine concentration in sewage is obtained；
S5：Calculate the yield per capita of two kinds of biomarkers of ammonia nitrogen and cotinine
S51：The data of yield per capita of the ammonia nitrogen obtain in the following way：
In formula, mNH4-NFor the yield per capita of ammonia nitrogen；mIFor the Q days interior ammonia nitrogen total releases by enumeration district (ED)；PITo adjust
Look into the resident population in area；
S52：The data of yield per capita of the cotinine obtain in the following way：
mCotinine=NCigarette×DNicotine×YNicotine×xCotinine
In formula, mCotinineFor cotinine yield per capita；NCigaretteFor daily amount of smoking per capita；DNicotineFor tobacco
The content of middle nicotine；YNicotineIt is human body to the intake of nicotine；xCotinineThe ratio being metabolized after human body is sucked for nicotine
Rate；
S6：The size of population of the sewage treatment plant region obtains in the following way：
In formula, P is sewage treatment plant's real time service size of population；C is the concentration of biomarker；F is the discharge of sewage；m
For the calculated data of yield, that is, S51 and S52 per capita of biomarker.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Any one skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (4)
1. a kind of size of population investigation method based on concentration of wastewater monitoring, it is characterised in that：Include the following steps：
S1：The region for determining the pre- estimation size of population, selects the sewage treatment plant in the region, acquires sewage plant into water sample
And sample is pre-processed；
S2：Select body metabolism product ammonia nitrogen and the metabolite cotinine of tobacco as biomarker；
S3：The ammonia nitrogen concentration in sewage is measured using National Standard Method, wherein national standard law regulation is surveyed using Berthelot spectrophotometry
Determine the ammonia nitrogen concentration in sewage；
S4：Cotinine concentration is measured using liquid chromatography mass instrument or gas chromatography-mass spectrometry；
S5：The yield per capita for calculating two kinds of biomarkers is calculated by biological marker method where obtaining the sewage treatment plant
The size of population in region.
2. a kind of size of population investigation method based on concentration of wastewater monitoring according to claim 1, it is further characterized in that：
The yield per capita of the ammonia nitrogen obtains in the following way：
In formula, mNH4-NFor the yield per capita of ammonia nitrogen；mIFor the Q days interior ammonia nitrogen total releases by enumeration district (ED)；PIFor enumeration district (ED)
Resident population.
3. a kind of size of population investigation method based on concentration of wastewater monitoring according to claim 1, it is further characterized in that：
The data of yield per capita of the cotinine obtain in the following way：
mCotinine=NCigarette×DNicotine×YNicotine×xCotinine
In formula, mCotinineFor cotinine yield per capita；NCigaretteFor daily amount of smoking per capita；DNicotineFor Buddhist nun in tobacco
The content of Gu Ding；YNicotineIt is human body to the intake of nicotine；xCotinineThe ratio being metabolized after human body is sucked for nicotine.
4. a kind of size of population investigation method based on concentration of wastewater monitoring according to claim 1, it is further characterized in that：
The size of population of the sewage treatment plant region obtains in the following way：
In formula, P is sewage treatment plant's real time service size of population, and C is the concentration of biomarker, and F is the discharge of sewage, and m makes a living
The quantity for the marker that the yield per capita of object marker is drained or generated for each person every day.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110456018A (en) * | 2019-08-16 | 2019-11-15 | 大连海事大学 | A method of based on the real-time continuous monitoring drug abuse situation of the comprehensive physical examination of water system |
| CN111487339A (en) * | 2020-04-22 | 2020-08-04 | 中国药科大学 | Method for evaluating regional population number based on concentration of 4-pyridoxic acid in sewage |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110456018A (en) * | 2019-08-16 | 2019-11-15 | 大连海事大学 | A method of based on the real-time continuous monitoring drug abuse situation of the comprehensive physical examination of water system |
| CN111487339A (en) * | 2020-04-22 | 2020-08-04 | 中国药科大学 | Method for evaluating regional population number based on concentration of 4-pyridoxic acid in sewage |
| CN111487339B (en) * | 2020-04-22 | 2022-02-11 | 中国药科大学 | Method for evaluating regional population number based on concentration of 4-pyridoxic acid in sewage |
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Application publication date: 20181102 |