CN108728510B - Method for promoting haematococcus pluvialis astaxanthin accumulation - Google Patents
Method for promoting haematococcus pluvialis astaxanthin accumulation Download PDFInfo
- Publication number
- CN108728510B CN108728510B CN201810575767.5A CN201810575767A CN108728510B CN 108728510 B CN108728510 B CN 108728510B CN 201810575767 A CN201810575767 A CN 201810575767A CN 108728510 B CN108728510 B CN 108728510B
- Authority
- CN
- China
- Prior art keywords
- haematococcus pluvialis
- astaxanthin
- glycerol
- accumulation
- light
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
Abstract
The invention belongs to the technical field of microalgae biology, and particularly relates to a method for promoting the accumulation of haematococcus pluvialis astaxanthin. When the haematococcus pluvialis cell culture reaches a plateau stage and light induction is carried out, a small molecular organic matter glycerol is added into a culture system, and then the accumulation of astaxanthin in the culture process is promoted. The invention discovers a small molecular organic matter-glycerin for the first time, can improve the content of haematococcus pluvialis astaxanthin under different light intensities, and is very favorable for comprehensively improving the production efficiency of outdoor haematococcus pluvialis astaxanthin.
Description
Technical Field
The invention belongs to the technical field of microalgae biology, and particularly relates to a method for promoting the accumulation of haematococcus pluvialis astaxanthin.
Background
The natural astaxanthin is a safe and efficient colorant and antioxidant, and has great application potential in the fields of aquatic products, cosmetics, feeds, foods, medicines and the like. Haematococcus pluvialis is the organism known in nature to contain the most astaxanthin and is considered to be the "concentrate" and the best biological source of natural astaxanthin. Haematococcus pluvialis astaxanthin is concerned by scholars at home and abroad due to high quality and high biological activity, and becomes a research hotspot for internationally producing natural astaxanthin. The cultivation of Haematococcus pluvialis has become the best way to obtain natural astaxanthin, and the industry of astaxanthin is greatly promoted.
Increasing the astaxanthin content in haematococcus pluvialis cells is an important way for increasing the production efficiency of astaxanthin. However, the astaxanthin content of the haematococcus pluvialis cultured by the existing outdoor pipeline and raceway pond photobioreactors is obviously different from the reported theoretical content. Furthermore, the intense light is a major factor inducing astaxanthin accumulation in Haematococcus, however, in the open (especially in rainy season) with frequent occurrence of continuous rainy weather, the maximum light intensity in the day is only 200. mu. mol/m2Less than s (the maximum daily light intensity for inducing astaxanthin accumulation outdoors in sunny days is 2000 mu mol/m2More than s). Insufficient light caused by rainy days delays or even inhibits the accumulation of astaxanthin of haematococcus pluvialis, and the production efficiency of astaxanthin is also greatly restricted. In contrast, it is now on a large scaleThere is no good measure in the production of haematococcus astaxanthin, and a technical breakthrough is urgently needed to be solved.
Disclosure of Invention
The invention aims to provide a method for promoting the accumulation of astaxanthin in haematococcus pluvialis so as to improve the production efficiency of astaxanthin.
In order to achieve the purpose, the invention adopts the technical scheme that:
a method for promoting the accumulation of astaxanthin in Haematococcus pluvialis comprises culturing Haematococcus pluvialis cells until the cell reaches a plateau stage, adding small molecular organic substance glycerol into the culture system during photoinduction, and further promoting the accumulation of astaxanthin in the culture process.
The micromolecular organic glycerol is added to a culture system during photoinduction in a mode of one time or multiple times.
The light intensity of the haematococcus pluvialis cells when the haematococcus pluvialis cells are placed under light to induce the accumulation of astaxanthin can be 100-4000 mu mol/m2/s。
When the micromolecular organic matter glycerol is added into a culture system for photoinduction at one time, the addition amount of the glycerol is 0.01-0.15 mol per liter of haematococcus pluvialis algae liquid;
or adding the mixture into a culture system for light induction for multiple times, wherein the addition amount of glycerol is 0.001-0.04 mol/L in haematococcus pluvialis algae solution, and adding 0.02-0.15 mol of glycerol into each liter of haematococcus pluvialis algae solution after haematococcus pluvialis cells become brown under light induction culture.
The invention has the advantages that:
the invention adds the economic and cheap micromolecular organic matter-glycerin which is widely distributed in organisms in the process of obtaining the haematococcus pluvialis astaxanthin, and the glycerol can improve the light intensity under different light intensities (100-2/s) the content of astaxanthin from Haematococcus pluvialis; the content of astaxanthin in haematococcus pluvialis can be increased under the conditions of strong light in sunny days and weak light in rainy days, and the comprehensive improvement of the production efficiency of the outdoor haematococcus pluvialis astaxanthin is facilitated.
Drawings
FIG. 1 provides an embodiment of the present inventionUnder low light conditions (200. mu. mol/m)2At/s), the effect of 0.014mol/L final glycerol on astaxanthin accumulation in Haematococcus pluvialis (control Haematococcus pluvialis without glycerol addition).
FIG. 2 shows the high light conditions (2500. mu. mol/m) provided by the examples of the present invention2S) effect of glycerol at a final concentration of 0.01mol/L on astaxanthin accumulation by Haematococcus pluvialis (control Haematococcus pluvialis without glycerol addition).
FIG. 3 shows the high light conditions (3000. mu. mol/m) provided by the examples of the present invention2At/s), the effect of glycerol at a final concentration of 0.02mol/L on astaxanthin accumulation by Haematococcus pluvialis (control Haematococcus pluvialis without glycerol addition).
FIG. 4 shows the intense light conditions (2000. mu. mol/m) provided by an example of the present invention2S) the effect of glycerol at an initial final concentration of 0.002mol/L and an additional final concentration of 0.02mol/L on the accumulation of astaxanthin from Haematococcus pluvialis on day 5 (arrow) (control Haematococcus pluvialis without glycerol addition).
Detailed Description
The following specific examples are provided to further illustrate the technical solutions of the present invention in detail. The invention is in no way limited thereto.
Example 1
At a light intensity of 150. mu. mol/m2Culturing haematococcus pluvialis cells under the conditions of the light-dark ratio of 14h/10h to reach a plateau stage, wherein the cell density is 50.89 ten thousand cells/mL. Diluting the haematococcus pluvialis cells to 14.22 ten thousand cells/mL with tap water, adding glycerol to the final concentration of 0.014mol/L, and adjusting the light intensity to 200 μmol/m2The astaxanthin accumulation is induced under the conditions of the light-dark ratio of 14h/10 h/s.
As can be seen from FIG. 1, from day 5 onwards, addition of glycerol at a time significantly increased the astaxanthin content of Haematococcus pluvialis compared to the control (glycerol-free group), indicating that glycerol promotes accumulation of Haematococcus pluvialis astaxanthin under low light.
Example 2
At a light intensity of 200. mu. mol/m2Culturing haematococcus pluvialis cells under the conditions of the light-dark ratio of 14h/10h to reach a plateau stage, wherein the cell density is 41.03 ten thousand cells/mL. Make the rain liveAdding glycerol directly into Haematococcus cells to final concentration of 0.01mol/L and light intensity of 2500 μmol/m2The astaxanthin accumulation is induced under the conditions of the light-dark ratio of 14h/10 h/s.
As can be seen from FIG. 2, from day 5 onward, addition of glycerol at a time significantly increased the astaxanthin content of Haematococcus pluvialis compared to the control (glycerol-free group), indicating that glycerol promotes accumulation of Haematococcus pluvialis astaxanthin under intense light.
Example 3
At a light intensity of 180. mu. mol/m2Culturing the haematococcus pluvialis cells under the conditions of the light-dark ratio of 14h/10h to reach a plateau stage, wherein the cell density is 52.34 ten thousand cells/mL. Diluting the haematococcus pluvialis cells to 20.12 ten thousand cells/mL with tap water, adding glycerol to a final concentration of 0.02mol/L and a light intensity of 3000 μmol/m2The astaxanthin accumulation is induced under the conditions of the light-dark ratio of 14h/10 h/s.
As can be seen from FIG. 3, from day 5 onward, addition of glycerol at a time significantly increased the astaxanthin content of Haematococcus pluvialis compared to the control (glycerol-free group), indicating that glycerol promotes accumulation of Haematococcus pluvialis astaxanthin under intense light.
Example 4
At a light intensity of 190. mu. mol/m2Culturing haematococcus pluvialis cells under the conditions of the light-dark ratio of 14h/10h to reach a plateau stage, wherein the cell density is 50.98 ten thousand cells/mL. Diluting the haematococcus pluvialis cells to 19.87 ten thousand cells/mL with tap water, adding glycerol to the final concentration of 0.002mol/L and the light intensity of 2000 mu mol/m2Inducing astaxanthin accumulation under the conditions of/s and light-dark ratio of 14h/10 h; meanwhile, glycerol with the final concentration of 0.02mol/L is added in the evening of the 5 th day of culture.
As shown in fig. 4, from day 5 onward, addition of glycerol in two portions significantly increased the astaxanthin content of haematococcus pluvialis compared to the control (glycerol-free group), indicating that addition of glycerol in multiple portions promoted accumulation of haematococcus pluvialis astaxanthin under intense light.
The above-mentioned embodiments are preferred applications of the present invention, but not intended to limit the invention in any way; for example, light-induced accumulation of astaxanthin is typically carried out after a plateau in production, but the invention can also be practiced at other times. In practice, the invention can be modified and practiced in different but equivalent manners apparent to those skilled in the art having the benefit of the teachings herein.
Claims (1)
1. A method for promoting the accumulation of astaxanthin in Haematococcus pluvialis is characterized by comprising the following steps: when the haematococcus pluvialis cell culture reaches a plateau stage and is subjected to light induction, adding a small molecular organic matter glycerol into a culture system, and further promoting the accumulation of astaxanthin in the culture process;
the light irradiation during the light induction is 200-2/s；
When the micromolecular organic matter glycerol is added into a culture system for photoinduction at one time, the addition amount of the glycerol is 0.01-0.02 mol per liter of haematococcus pluvialis algae liquid;
or adding the mixture into a culture system for light induction for multiple times, wherein the addition amount of glycerol is 0.002mol/L in haematococcus pluvialis algae solution, and adding 0.02mol of glycerol into each liter of haematococcus pluvialis algae solution after haematococcus pluvialis cells become brown under light induction culture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810575767.5A CN108728510B (en) | 2018-06-06 | 2018-06-06 | Method for promoting haematococcus pluvialis astaxanthin accumulation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810575767.5A CN108728510B (en) | 2018-06-06 | 2018-06-06 | Method for promoting haematococcus pluvialis astaxanthin accumulation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108728510A CN108728510A (en) | 2018-11-02 |
| CN108728510B true CN108728510B (en) | 2021-07-23 |
Family
ID=63932492
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810575767.5A Active CN108728510B (en) | 2018-06-06 | 2018-06-06 | Method for promoting haematococcus pluvialis astaxanthin accumulation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108728510B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108504619B (en) * | 2018-06-06 | 2021-06-01 | 中国科学院海洋研究所 | Method for relieving light inhibition of haematococcus pluvialis |
| CN113881749B (en) * | 2021-11-11 | 2023-06-20 | 中国科学院海洋研究所 | Method for rapidly promoting astaxanthin accumulation in haematococcus pluvialis |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107365826A (en) * | 2017-09-18 | 2017-11-21 | 深圳市德和生物科技有限公司 | A kind of method of regulating astaxanthin accumulation |
-
2018
- 2018-06-06 CN CN201810575767.5A patent/CN108728510B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107365826A (en) * | 2017-09-18 | 2017-11-21 | 深圳市德和生物科技有限公司 | A kind of method of regulating astaxanthin accumulation |
Non-Patent Citations (3)
| Title |
|---|
| A strategy for stimulating astaxanthin and lipid production in Haematococcus pluvialis by exogenous glycerol application under low light;Litao Zhang等;《Algal Research》;20200331;第46卷;第1-6页 * |
| Ethanol induced astaxanthin accumulation and transcriptional expression of carotenogenic genes in Haematococcus pluvialis;Zewen Wen等;《Enzyme and Microbial Technology》;20150612;第78卷;第10-17页 * |
| Metabolomic and network analysis of astaxanthin-producing Haematococcus pluvialis under various stress conditions;Yingxue Su等;《Bioresource Technology》;20140810;第170卷;第522-529页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108728510A (en) | 2018-11-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wan et al. | Sequential heterotrophy–dilution–photoinduction cultivation of Haematococcus pluvialis for efficient production of astaxanthin | |
| JP6414904B2 (en) | Process for the production of microalgae, cyanobacteria and their metabolites | |
| Wang et al. | Microalgae cultivation in photobioreactors: An overview of light characteristics | |
| Wan et al. | The effective photoinduction of Haematococcus pluvialis for accumulating astaxanthin with attached cultivation | |
| Guccione et al. | Chlorella for protein and biofuels: from strain selection to outdoor cultivation in a Green Wall Panel photobioreactor | |
| Barghbani et al. | Investigating the effects of several parameters on the growth of Chlorella vulgaris using Taguchi's experimental approach | |
| Van Wagenen et al. | Comparison of mixotrophic to cyclic autotrophic/heterotrophic growth strategies to optimize productivity of Chlorella sorokiniana | |
| CN105648018B (en) | A kind of method for promoting Haematococcus pluvialis production astaxanthin using butylated hydroxy anisole | |
| CN109536484B (en) | Method for improving biomass and metabolite of chrysophyceae by magnetic field intervention | |
| Li et al. | Effects of strain, nutrients concentration and inoculum size on microalgae culture for bioenergy from post hydrothermal liquefaction wastewater | |
| CN108728510B (en) | Method for promoting haematococcus pluvialis astaxanthin accumulation | |
| Liu et al. | Biomass productivity of Scenedesmus dimorphus (Chlorophyceae) was improved by using an open pond–photobioreactor hybrid system | |
| Imaizumi et al. | Lumostatic operation controlled by the optimum light intensity per dry weight for the effective production of Chlorella zofingiensis in the high cell density continuous culture | |
| Pan-utai et al. | Photoautotrophic cultivation of Arthrospira maxima for protein accumulation under minimum nutrient availability | |
| Chen et al. | Strategies to improve oil/lipid production of microalgae in outdoor cultivation using vertical tubular-type photobioreactors | |
| EP3498855B1 (en) | Process for the cultivation of microalgae for the production of starch | |
| CN106868085A (en) | A kind of method for promoting haematococcus pluvialis rapid conversion to accumulate astaxanthin | |
| CN106480155B (en) | Method suitable for promoting haematococcus pluvialis to produce astaxanthin under high-temperature condition | |
| CN109679853A (en) | The method for improving haematococcus pluvialis biomass and astaxanthin yield using fulvic acid | |
| CN107189946B (en) | Method for improving astaxanthin yield by avoiding microalgae photoinhibition | |
| AU2020101953A4 (en) | A method of cultivating microalgae with high oil content | |
| KR101797070B1 (en) | Culture compositions for promoting growth of spirulina and the method culturing for promoting growth of spirulina by using the same | |
| CN113512575B (en) | Method for promoting astaxanthin accumulation in haematococcus pluvialis | |
| CN108504619B (en) | Method for relieving light inhibition of haematococcus pluvialis | |
| Yang et al. | Water-saving analysis on an effective water reuse system in biodiesel feedstock production based on Chlorella zofingiensis fed-batch cultivation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |