CN108728510A - A method of promoting Astaxanthin In Haematococcus Pluvialis accumulation - Google Patents
A method of promoting Astaxanthin In Haematococcus Pluvialis accumulation Download PDFInfo
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- CN108728510A CN108728510A CN201810575767.5A CN201810575767A CN108728510A CN 108728510 A CN108728510 A CN 108728510A CN 201810575767 A CN201810575767 A CN 201810575767A CN 108728510 A CN108728510 A CN 108728510A
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- astaxanthin
- haematococcus pluvialis
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- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
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Abstract
The invention belongs to technical field of microalga biology, and in particular to a method of promoting Astaxanthin In Haematococcus Pluvialis accumulation.Haematococcus pluvialis cell culture reaches plateau, when carrying out photoinduction, small organic molecule glycerine is added into cultivating system, and then promote the accumulation of astaxanthin in incubation.Present invention firstly discovers that small organic molecule-glycerine, can be improved the content of Astaxanthin In Haematococcus Pluvialis under different light intensity, it is advantageous to comprehensive improve outdoor Astaxanthin In Haematococcus Pluvialis production efficiency.
Description
Technical field
The invention belongs to technical field of microalga biology, and in particular to a kind of side promoting Astaxanthin In Haematococcus Pluvialis accumulation
Method.
Background technology
Natural astaxanthin is a kind of safe and efficient colorant and antioxidant, in aquatic products, cosmetics, feed, food, doctor
The fields such as medicine have very big application potential.Haematococcus pluvialis is the biology that known astaxanthin-containing is most in nature, is seen
" concentrate " as natural astaxanthin and best biological source.Astaxanthin In Haematococcus Pluvialis is living with its high-quality and high biology
Property paid close attention to by domestic and foreign scholars, become the research hotspot of the production of natural astaxanthin in the world.Cultivation haematococcus pluvialis has become
To obtain the optimal path of natural astaxanthin, carrying forward vigorously for astaxanthin industry is obtained.
It is the important channel for increasing production of astaxanthin efficiency to improve the intracellular content astaxanthin of haematococcus.However, current family
Outer Tube and the haematococcus of raceway pond bioreactor culture, it is apparent poor that content astaxanthin exists from the theoretical content of report
Away from.In addition, strong light is the Main Factors for inducing haematococcus astaxanthin accumulation, however, being frequently occurred outdoors (especially in rainy season)
Continuous rainy weather, day highest light intensity but only have 200 μm of ol/m2/ s or less (the day highests of fine day open air induction astaxanthin accumulation
2000 μm of ol/m of light intensity2/ s or more).Insufficient light caused by rainy weather delays even to inhibit the product of Astaxanthin In Haematococcus Pluvialis
It is tired, also greatly restrict production of astaxanthin efficiency.In this regard, at present without very in the production of scale Astaxanthin In Haematococcus Pluvialis
Good measure, it would be highly desirable to which technological break-through is resolved.
Invention content
The object of the present invention is to provide a kind of accumulation of promotion Astaxanthin In Haematococcus Pluvialis, to improve production of astaxanthin efficiency
Method.
To achieve the above object, the invention adopts a technical scheme as：
A method of promote Astaxanthin In Haematococcus Pluvialis accumulation, haematococcus pluvialis cell culture to reach plateau, carries out
When photoinduction, small organic molecule glycerine is added into cultivating system, and then promote the accumulation of astaxanthin in incubation.
The mode of the small organic molecule glycerine one or more times is added in cultivating system when carrying out photoinduction.
Light intensity haematococcus pluvialis cell being placed under light when inducing astaxanthin accumulation can be 100-4000 μm of ol/
m2/s。
When the small organic molecule glycerine is by being disposably added in cultivating system when carrying out photoinduction, glycerine adds
Dosage is 0.01-0.15mol in every liter of haematococcus pluvialis algae solution；
Or, being added in cultivating system when carrying out photoinduction by multiple mode, the additive amount of glycerine is that every liter of rain is given birth to
0.001-0.04mol/L in haematococcus algae solution, after haematococcus pluvialis cell becomes brown under photoinduction culture, then adds
Throw the glycerine of 0.02-0.15mol in every liter of haematococcus pluvialis algae solution.
The present invention is had the advantage that：
The present invention added during obtaining Astaxanthin In Haematococcus Pluvialis it is widely distributed in vivo, it is relatively cheap small
(100-4000 μm of ol/m can be improved under different light intensity in molecular organic-glycerine2/ s) Astaxanthin In Haematococcus Pluvialis content；
No matter the content of Determination of Astaxanthin in Haematococcus Pluvialis all can be improved under fine day intense light conditions or under rainy days low light condition, in turn
Be conducive to the outdoor Astaxanthin In Haematococcus Pluvialis production efficiency of comprehensive raising.
Description of the drawings
Fig. 1 is low light condition provided in an embodiment of the present invention (200 μm of ol/m2/ s) under, final concentration 0.014mol/L's is sweet
The influence that oil accumulates Astaxanthin In Haematococcus Pluvialis (control is to be not added with the haematococcus pluvialis of glycerine).
Fig. 2 is intense light conditions provided in an embodiment of the present invention (2500 μm of ol/m2/ s) under, final concentration 0.01mol/L's is sweet
The influence that oil accumulates Astaxanthin In Haematococcus Pluvialis (control is to be not added with the haematococcus pluvialis of glycerine).
Fig. 3 is intense light conditions provided in an embodiment of the present invention (3000 μm of ol/m2/ s) under, final concentration 0.02mol/L's is sweet
The influence that oil accumulates Astaxanthin In Haematococcus Pluvialis (control is to be not added with the haematococcus pluvialis of glycerine).
Fig. 4 is intense light conditions provided in an embodiment of the present invention (2000 μm of ol/m2/ s) under, initial final concentration 0.002mol/L
Glycerine and Astaxanthin In Haematococcus Pluvialis is accumulated in the glycerine of the 5th day additional final concentration 0.02mol/L (at arrow) influence
(control is to be not added with the haematococcus pluvialis of glycerine).
Specific implementation mode
Example is used for the technical solution that present invention be described in more detail in detail below.But the present invention is only limitted to absolutely not
This.
Embodiment 1
It is 150 μm of ol/m in light intensity2Culture haematococcus pluvialis cell reaches flat under conditions of/s, Light To Dark Ratio are 14h/10h
Platform phase, cell density are 50.89 ten thousand cell/mL.This haematococcus pluvialis cell is diluted to 14.22 ten thousand carefully with tap water
Born of the same parents/mL, addition glycerine to final concentration of 0.014mol/L are 200 μm of ol/m in light intensity2/ s, the condition that Light To Dark Ratio is 14h/10h
Lower induction astaxanthin accumulation.
As seen from Figure 1, since the 5th day, compared with control (non-glycerol adding group), the disposable glycerine that adds significantly improves
The content of Astaxanthin In Haematococcus Pluvialis shows that glycerine can promote the accumulation of Astaxanthin In Haematococcus Pluvialis under dim light.
Embodiment 2
It is 200 μm of ol/m in light intensity2Culture haematococcus pluvialis cell reaches flat under conditions of/s, Light To Dark Ratio are 14h/10h
Platform phase, cell density are 41.03 ten thousand cell/mL.This haematococcus pluvialis cell is directly added to glycerine to final concentration of
0.01mol/L is 2500 μm of ol/m in light intensity2/ s, Light To Dark Ratio induce astaxanthin accumulation under conditions of being 14h/10h.
From Figure 2 it can be seen that since the 5th day, compared with control (non-glycerol adding group), the disposable glycerine that adds significantly improves
The content of Astaxanthin In Haematococcus Pluvialis shows that glycerine can promote the accumulation of Astaxanthin In Haematococcus Pluvialis under strong light.
Embodiment 3
It is 180 μm of ol/m in light intensity2Culture haematococcus pluvialis cell reaches flat under conditions of/s, Light To Dark Ratio are 14h/10h
Platform phase, cell density are 52.34 ten thousand cell/mL.This haematococcus pluvialis cell is diluted to 20.12 ten thousand carefully with tap water
Born of the same parents/mL, addition glycerine to final concentration of 0.02mol/L are 3000 μm of ol/m in light intensity2/ s, the condition that Light To Dark Ratio is 14h/10h
Lower induction astaxanthin accumulation.
As seen from Figure 3, since the 5th day, compared with control (non-glycerol adding group), the disposable glycerine that adds significantly improves
The content of Astaxanthin In Haematococcus Pluvialis shows that glycerine can promote the accumulation of Astaxanthin In Haematococcus Pluvialis under strong light.
Embodiment 4
It is 190 μm of ol/m in light intensity2Culture haematococcus pluvialis cell reaches flat under conditions of/s, Light To Dark Ratio are 14h/10h
Platform phase, cell density are 50.98 ten thousand cell/mL.This haematococcus pluvialis cell is diluted to 19.87 ten thousand carefully with tap water
Born of the same parents/mL, addition glycerine to final concentration of 0.002mol/L are 2000 μm of ol/m in light intensity2/ s, the item that Light To Dark Ratio is 14h/10h
Astaxanthin accumulation is induced under part；Simultaneously in the 5th day glycerine for adding final concentration of 0.02mol/L at night of culture.
As shown in figure 4, since the 5th day, compared with control (non-glycerol adding group), glycerine is added at twice and is significantly improved
The content of Astaxanthin In Haematococcus Pluvialis shows that Astaxanthin In Haematococcus Pluvialis under strong light can be promoted by adding glycerine by multiple mode
Accumulation.
Embodiment described above is patent preferred application case of the present invention, but is not generated in any form to the present invention
Limitation；For example, general in production carry out photoinduction accumulation astaxanthin all after plateau, but can also be real in other periods
The existing present invention.In actual application, without departing from the scope of the present invention, when the technology using the disclosure above
Content makes a little change or the equivalent embodiment for being modified to equivalent variations.
Claims (4)
1. a kind of method promoting Astaxanthin In Haematococcus Pluvialis accumulation, it is characterised in that：Haematococcus pluvialis cell culture reaches flat
When carrying out photoinduction, small organic molecule glycerine was added into cultivating system, and then promotes astaxanthin in incubation the platform phase
Accumulation.
2. by the method for promoting Astaxanthin In Haematococcus Pluvialis accumulation described in claim 1, it is characterised in that：The small molecule is organic
The mode of object glycerine one or more times is added in cultivating system when carrying out photoinduction.
3. by the method for promoting Astaxanthin In Haematococcus Pluvialis accumulation described in claims 1 or 2, it is characterised in that：It is described to give birth to rain
The light intensity that haematococcus cell is placed under light when inducing astaxanthin accumulation can be 100-4000 μm of ol/m2/s。
4. by the method for promoting Astaxanthin In Haematococcus Pluvialis accumulation described in claim 3, it is characterised in that：The small molecule is organic
When object glycerine is by being disposably added in cultivating system when carrying out photoinduction, the additive amount of glycerine is every liter of haematococcus pluvialis algae
0.01-0.15mol in liquid；
Or, being added in cultivating system when carrying out photoinduction by multiple mode, the additive amount of glycerine is that every liter of rain gives birth to red ball
0.001-0.04mol/L in algae algae solution, after haematococcus pluvialis cell becomes brown under photoinduction culture, then additional throwing is every
Rise the glycerine of 0.02-0.15mol in haematococcus pluvialis algae solution.
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| CN201810575767.5A CN108728510B (en) | 2018-06-06 | 2018-06-06 | Method for promoting haematococcus pluvialis astaxanthin accumulation |
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| CN201810575767.5A CN108728510B (en) | 2018-06-06 | 2018-06-06 | Method for promoting haematococcus pluvialis astaxanthin accumulation |
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| CN108728510B CN108728510B (en) | 2021-07-23 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108504619A (en) * | 2018-06-06 | 2018-09-07 | 中国科学院海洋研究所 | A method of alleviating haematococcus pluvialis Xanthophyll cycle |
| CN113881749A (en) * | 2021-11-11 | 2022-01-04 | 中国科学院海洋研究所 | Method for rapidly promoting accumulation of astaxanthin in haematococcus pluvialis |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107365826A (en) * | 2017-09-18 | 2017-11-21 | 深圳市德和生物科技有限公司 | A kind of method of regulating astaxanthin accumulation |
-
2018
- 2018-06-06 CN CN201810575767.5A patent/CN108728510B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107365826A (en) * | 2017-09-18 | 2017-11-21 | 深圳市德和生物科技有限公司 | A kind of method of regulating astaxanthin accumulation |
Non-Patent Citations (3)
| Title |
|---|
| LITAO ZHANG等: "A strategy for stimulating astaxanthin and lipid production in Haematococcus pluvialis by exogenous glycerol application under low light", 《ALGAL RESEARCH》 * |
| YINGXUE SU等: "Metabolomic and network analysis of astaxanthin-producing Haematococcus pluvialis under various stress conditions", 《BIORESOURCE TECHNOLOGY》 * |
| ZEWEN WEN等: "Ethanol induced astaxanthin accumulation and transcriptional expression of carotenogenic genes in Haematococcus pluvialis", 《ENZYME AND MICROBIAL TECHNOLOGY》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108504619A (en) * | 2018-06-06 | 2018-09-07 | 中国科学院海洋研究所 | A method of alleviating haematococcus pluvialis Xanthophyll cycle |
| CN108504619B (en) * | 2018-06-06 | 2021-06-01 | 中国科学院海洋研究所 | Method for relieving light inhibition of haematococcus pluvialis |
| CN113881749A (en) * | 2021-11-11 | 2022-01-04 | 中国科学院海洋研究所 | Method for rapidly promoting accumulation of astaxanthin in haematococcus pluvialis |
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