CN108722981B - Method for quickly removing paraffin in dehydration box - Google Patents

Method for quickly removing paraffin in dehydration box Download PDF

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CN108722981B
CN108722981B CN201810357992.1A CN201810357992A CN108722981B CN 108722981 B CN108722981 B CN 108722981B CN 201810357992 A CN201810357992 A CN 201810357992A CN 108722981 B CN108722981 B CN 108722981B
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paraffin
dewatering box
box
dehydration
weight
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CN108722981A (en
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朱清
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BENGBU MEDICAL COLLEGE
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B08CLEANING
    • B08BCLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/04Cleaning involving contact with liquid
    • B08B3/08Cleaning involving contact with liquid the liquid having chemical or dissolving effect
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B08CLEANING
    • B08BCLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/04Cleaning involving contact with liquid
    • B08B3/10Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/37Polymers
    • C11D3/3703Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C11D3/373Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds containing silicones
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D7/00Compositions of detergents based essentially on non-surface-active compounds
    • C11D7/22Organic compounds
    • C11D7/26Organic compounds containing oxygen
    • C11D7/261Alcohols; Phenols
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D7/00Compositions of detergents based essentially on non-surface-active compounds
    • C11D7/22Organic compounds
    • C11D7/26Organic compounds containing oxygen
    • C11D7/266Esters or carbonates
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D7/00Compositions of detergents based essentially on non-surface-active compounds
    • C11D7/22Organic compounds
    • C11D7/28Organic compounds containing halogen
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D7/00Compositions of detergents based essentially on non-surface-active compounds
    • C11D7/22Organic compounds
    • C11D7/32Organic compounds containing nitrogen
    • C11D7/3245Aminoacids
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D7/00Compositions of detergents based essentially on non-surface-active compounds
    • C11D7/22Organic compounds
    • C11D7/34Organic compounds containing sulfur

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Emergency Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Detergent Compositions (AREA)
  • Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)
  • Compositions Of Macromolecular Compounds (AREA)

Abstract

The invention discloses a method for quickly removing paraffin in a dehydration box, which comprises the following operation steps: (1) uniformly mixing in-situ polymerized nano hybrid silicone oil, sodium methallyl sulfonate and deionized water, and then carrying out ultrasonic treatment to prepare a dissociation agent; (2) adding 1, 2-cyclohexanediamine tetraacetic acid, D-methyl lactate and diethylene glycol chloroformate into ethanol, mixing and stirring uniformly, adding a dissociating agent, and continuously stirring and mixing uniformly to obtain paraffin removing liquid; (3) and (3) putting the dewatering box into warm water, taking out the dewatering box after soaking, quickly absorbing water on the surface of the dewatering box by using filter paper, spraying paraffin removing liquid on the surface of the dewatering box, putting the dewatering box into the warm water again after 5-7min, taking out the dewatering box after soaking, washing by using water again, naturally drying, and finishing paraffin removal in the dewatering box. The method provided by the invention can effectively remove the paraffin adhered on the dehydration box, has no residue, and cannot influence the reuse of the dehydration box.

Description

Method for quickly removing paraffin in dehydration box
Technical Field
The invention belongs to the technical field of cleaning of medical detection instruments, and particularly relates to a method for quickly removing paraffin in a dehydration box.
Background
The paraffin tissue section technology is an indispensable important technology in life science research, teaching and diagnosis, in the paraffin tissue section manufacturing process, especially in the paraffin tissue section manufacturing process of pathology diagnosis, teaching and scientific research experiments, the dehydration box is one of important equipment which can be repeatedly utilized, various tissue specimens need to be respectively placed in the dehydration boxes after being numbered and taken, the main purpose of the dehydration box is to distinguish the similar tissues of different individual sources, and simultaneously, the selected tissues are prevented from being lost. The fixation, dehydration, transparence, wax dipping and the like of each batch of tissues are carried out in a dehydration box, after the tissues are embedded, a large amount of solid paraffin can be adhered to the dehydration box entering a wax cylinder along with the tissues, the solid paraffin adhered to the dehydration box recycled in the next batch of tissue slice manufacturing process can be dissolved by organic solvents such as xylene for tissue transparence and the like in the tissue dehydration and transparence processes, and the xylene with the paraffin dissolved can be seriously influenced in purity and tissue transparency effect, so that the tissue slice manufacturing result is influenced. Therefore, after tissue embedding, the solid paraffin adhered to the dehydration box must be removed by treatment. If the paraffin solidified on the dehydration box is not processed or is not processed well, the normal use of the dehydration box in the next batch of tissue slice production is influenced. Some paraffin tissue section production units use a disposable dehydration box, which avoids the trouble of removing solidified paraffin adhered on the dehydration box, but causes a great deal of paraffin waste and causes the pollution of paraffin to the environment.
Disclosure of Invention
In order to solve the technical problem, the invention provides a method for quickly removing paraffin in a dehydration box.
The invention is realized by the following technical scheme.
A method for quickly removing paraffin from a dehydration box comprises the following operation steps:
(1) uniformly mixing in-situ polymerization nano hybrid silicone oil, sodium methallyl sulfonate and deionized water according to the mass ratio of 10-15:1:20, and performing ultrasonic treatment for 28-32min to prepare a dissociation agent;
(2) adding 14-19 parts by weight of 1, 2-cyclohexanediamine tetraacetic acid, 3-6 parts by weight of D-methyl lactate and 16-20 parts by weight of diethylene glycol chloroformate into 75-85 parts by weight of ethanol, uniformly mixing and stirring, adding 8-10 parts by weight of the dissociation agent prepared in the step (1), continuously stirring and uniformly mixing to prepare paraffin removal liquid;
(3) putting the dewatering box into warm water of 40-50 ℃, taking out the dewatering box after soaking for 3-5min, quickly absorbing water on the surface of the dewatering box by using filter paper, then spraying paraffin removal liquid on the surface of the dewatering box, putting the dewatering box into warm water of 40-50 ℃ again after 5-7min, taking out the dewatering box after soaking for 10-12min, washing by using water, naturally drying, and finishing paraffin removal in the dewatering box.
Specifically, in the step (1), the purity of the in-situ polymerized nano hybrid silicone oil is 99.9%.
Specifically, in the step (1), the frequency of the ultrasonic treatment is 28-30 kHz.
Specifically, in the step (2), the mass fraction of the ethanol is 90-95%.
Specifically, in the step (3), the spraying amount of the paraffin wax removing liquid is 350-2
According to the technical scheme, the beneficial effects of the invention are as follows:
the method for quickly removing the paraffin in the dehydration box is simple to operate, is green and environment-friendly, can effectively remove the paraffin adhered to the dehydration box, has no residue, and cannot influence the reuse of the dehydration box. The dissociating agent prepared in the step (1) can enable the acting force between paraffin molecules to be rapidly reduced, and can effectively avoid the phenomenon that paraffin is blocked in a sewer; after the synergistic effect of the 1, 2-cyclohexanediamine tetraacetic acid, the D-methyl lactate and the diglycol chloroformate, the permeability of the dissociating agent in the solid paraffin can be effectively improved, the paraffin removing effect is greatly improved, the paraffin at the dead angle position in the dehydration box also has a good removing effect, no residue exists, and the reuse of the dehydration box cannot be influenced.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the following examples further illustrate the present invention in detail. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1
A method for quickly removing paraffin from a dehydration box comprises the following operation steps:
(1) uniformly mixing in-situ polymerization nano hybrid silicone oil, sodium methallyl sulfonate and deionized water according to the mass ratio of 10:1:20, and performing ultrasonic treatment for 28min to prepare a dissociation agent;
(2) adding 14 parts by weight of 1, 2-cyclohexanediamine tetraacetic acid, 3 parts by weight of D-methyl lactate and 16 parts by weight of diethylene chloroformate into 75 parts by weight of ethanol, uniformly mixing and stirring, adding 8 parts by weight of the dissociation agent prepared in the step (1), continuously stirring and uniformly mixing to prepare paraffin removal liquid;
(3) putting the dewatering box into warm water at 40 ℃, taking out the dewatering box after soaking for 3min, quickly absorbing water on the surface of the dewatering box by using filter paper, then spraying paraffin removing liquid on the surface of the dewatering box, putting the dewatering box into the warm water at 40 ℃ again after 5min, taking out the dewatering box after soaking for 10min, washing by using water, naturally drying, and finishing paraffin removal in the dewatering box.
Specifically, in the step (1), the purity of the in-situ polymerized nano hybrid silicone oil is 99.9%.
Specifically, in the step (1), the frequency of the ultrasonic treatment is 28 kHz.
Specifically, in the step (2), the mass fraction of ethanol is 90%.
Specifically, in the step (3), the spraying amount of the paraffin wax removing liquid is 350ml/m2
Example 2
A method for quickly removing paraffin from a dehydration box comprises the following operation steps:
(1) uniformly mixing in-situ polymerization nano hybrid silicone oil, sodium methallyl sulfonate and deionized water according to the mass ratio of 13:1:20, and performing ultrasonic treatment for 30min to prepare a dissociation agent;
(2) adding 17 parts by weight of 1, 2-cyclohexanediamine tetraacetic acid, 5 parts by weight of D-methyl lactate and 18 parts by weight of diethylene chloroformate into 80 parts by weight of ethanol, mixing and stirring uniformly, adding 9 parts by weight of the dissociation agent prepared in the step (1), continuously stirring and mixing uniformly to prepare paraffin removal liquid;
(3) putting the dewatering box into warm water at 45 ℃, taking out the dewatering box after soaking for 4min, quickly absorbing water on the surface of the dewatering box by using filter paper, then spraying paraffin removing liquid on the surface of the dewatering box, putting the dewatering box into the warm water at 45 ℃ again after 6min, taking out the dewatering box after soaking for 11min, washing by using water, naturally drying, and finishing paraffin removal in the dewatering box.
Specifically, in the step (1), the purity of the in-situ polymerized nano hybrid silicone oil is 99.9%.
Specifically, in the step (1), the frequency of the ultrasonic treatment is 29 kHz.
Specifically, in the step (2), the mass fraction of ethanol is 93%.
Specifically, in the step (3), the spraying amount of the paraffin wax removing liquid is 380ml/m2
Example 3
A method for quickly removing paraffin from a dehydration box comprises the following operation steps:
(1) uniformly mixing in-situ polymerization nano hybrid silicone oil, sodium methallyl sulfonate and deionized water according to the mass ratio of 15:1:20, and performing ultrasonic treatment for 32min to prepare a dissociation agent;
(2) adding 19 parts by weight of 1, 2-cyclohexanediamine tetraacetic acid, 6 parts by weight of D-methyl lactate and 20 parts by weight of diethylene chloroformate into 85 parts by weight of ethanol, uniformly mixing and stirring, adding 10 parts by weight of the dissociation agent prepared in the step (1), continuously stirring and uniformly mixing to prepare paraffin removal liquid;
(3) putting the dewatering box into warm water at 50 ℃, taking out the dewatering box after soaking for 5min, quickly absorbing water on the surface of the dewatering box by using filter paper, then spraying paraffin removing liquid on the surface of the dewatering box, putting the dewatering box into the warm water at 50 ℃ again after 7min, taking out the dewatering box after soaking for 12min, washing by using water, naturally drying, and finishing paraffin removal in the dewatering box.
Specifically, in the step (1), the purity of the in-situ polymerized nano hybrid silicone oil is 99.9%.
Specifically, in the step (1), the frequency of the ultrasonic treatment is 30 kHz.
Specifically, in the step (2), the mass fraction of ethanol is 95%.
Specifically, in the step (3), the spraying amount of the paraffin wax removing liquid is 400ml/m2
Comparative example 1
In the step (2), the cleavage agent prepared in the step (1) was not added, and the remaining operation steps were completely the same as those in example 1.
The same batch of dewatering boxes having the same degree of paraffin contamination were subjected to cleaning tests using the methods of the examples and comparative examples, respectively, and the test results are shown in table 1:
TABLE 1 dehydration Box Paraffin removal Effect
Figure 220181DEST_PATH_IMAGE002
It is to be understood that the invention is not limited to the examples described above, but that modifications and variations may be effected thereto by those of ordinary skill in the art in light of the foregoing description, and that all such modifications and variations are intended to be within the scope of the invention as defined by the appended claims.

Claims (5)

1. A method for quickly removing paraffin from a dehydration box is characterized by comprising the following operation steps:
(1) uniformly mixing in-situ polymerization nano hybrid silicone oil, sodium methallyl sulfonate and deionized water according to the mass ratio of 10-15:1:20, and performing ultrasonic treatment for 28-32min to prepare a dissociation agent;
(2) adding 14-19 parts by weight of 1, 2-cyclohexanediamine tetraacetic acid, 3-6 parts by weight of D-methyl lactate and 16-20 parts by weight of diethylene glycol chloroformate into 75-85 parts by weight of ethanol, uniformly mixing and stirring, adding 8-10 parts by weight of the dissociation agent prepared in the step (1), continuously stirring and uniformly mixing to prepare paraffin removal liquid;
(3) putting the dewatering box into warm water of 40-50 ℃, taking out the dewatering box after soaking for 3-5min, quickly absorbing water on the surface of the dewatering box by using filter paper, then spraying paraffin removal liquid on the surface of the dewatering box, putting the dewatering box into warm water of 40-50 ℃ again after 5-7min, taking out the dewatering box after soaking for 10-12min, washing by using water, naturally drying, and finishing paraffin removal in the dewatering box.
2. The method for rapidly removing paraffin from a dehydration box according to claim 1, characterized in that in the step (1), the purity of the in-situ polymerized nano hybrid silicone oil is 99.9%.
3. The method for rapidly removing paraffin from a dewatering box according to claim 1, wherein in the step (1), the ultrasonic treatment frequency is 28-30 kHz.
4. The method for rapidly removing paraffin from a dehydration box according to claim 1, wherein the mass fraction of ethanol in the step (2) is 90-95%.
5. The method as claimed in claim 1, wherein the paraffin removal liquid is sprayed in an amount of 350-400ml/m in the step (3)2
CN201810357992.1A 2018-04-20 2018-04-20 Method for quickly removing paraffin in dehydration box Active CN108722981B (en)

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4089703A (en) * 1976-12-23 1978-05-16 White Chemical Company, Inc. Hot detergent process
JP2003313595A (en) * 2002-04-25 2003-11-06 Shinko Pantec Co Ltd Method for decontaminating organic halogen contaminant and decontamination system for the same
CN103614083A (en) * 2013-12-03 2014-03-05 沈阳化工大学 Automobile cleaning water wax and preparation method thereof
CN103980939A (en) * 2014-05-13 2014-08-13 山西兰花科技创业股份有限公司 Method of rapidly eliminating "wax precipitation" in alcoholization and alkylation systems
CN105419756A (en) * 2015-10-28 2016-03-23 中国石油化工股份有限公司 Wax removal and inhibition composition, water-soluble wax removal and inhibition agent and preparation method thereof
CN107144456A (en) * 2017-06-20 2017-09-08 广西壮族自治区肿瘤防治研究所 A kind of hydrogel support of paraffin pathological section tissue and lipid remove processing method
CN107429205A (en) * 2015-03-06 2017-12-01 弗洛泰克化学有限责任公司 Composition and method for clean surface and other application

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4089703A (en) * 1976-12-23 1978-05-16 White Chemical Company, Inc. Hot detergent process
JP2003313595A (en) * 2002-04-25 2003-11-06 Shinko Pantec Co Ltd Method for decontaminating organic halogen contaminant and decontamination system for the same
CN103614083A (en) * 2013-12-03 2014-03-05 沈阳化工大学 Automobile cleaning water wax and preparation method thereof
CN103980939A (en) * 2014-05-13 2014-08-13 山西兰花科技创业股份有限公司 Method of rapidly eliminating "wax precipitation" in alcoholization and alkylation systems
CN107429205A (en) * 2015-03-06 2017-12-01 弗洛泰克化学有限责任公司 Composition and method for clean surface and other application
CN105419756A (en) * 2015-10-28 2016-03-23 中国石油化工股份有限公司 Wax removal and inhibition composition, water-soluble wax removal and inhibition agent and preparation method thereof
CN107144456A (en) * 2017-06-20 2017-09-08 广西壮族自治区肿瘤防治研究所 A kind of hydrogel support of paraffin pathological section tissue and lipid remove processing method

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