CN108716391A - A method of it recovers the oil and is regulated and controled with endogenous microbes group - Google Patents
A method of it recovers the oil and is regulated and controled with endogenous microbes group Download PDFInfo
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- E—FIXED CONSTRUCTIONS
- E21—EARTH DRILLING; MINING
- E21B—EARTH DRILLING, e.g. DEEP DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
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- E—FIXED CONSTRUCTIONS
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Abstract
The invention belongs to technical field of tertiary oil recovery, and in particular to a kind of regulation and control method recovered the oil with endogenous microbes group.The present invention carries out the screening of experiment oil reservoir first, simulation core parameter is determined according to the condition of experiment oil reservoir, and then carry out the physical simulation experiment of experiment oil reservoir, microbiologic population's regularity of distribution is determined according to experiment reservoir physical simulation experimental result, to clear different type microorganism activator injection amount and live injection technology, field test is finally carried out.The present invention can effectively different types of microorganism in activation experiment oil reservoir, simultaneously effective reduce the cost of investment of microorganism activator, to realize the recovery ratio for increasing substantially experiment oil reservoir, field test results are apparent, input-output ratio 1:12 or more, it improves recovery ratio and is more than 20%.
Description
Technical field
The invention belongs to technical field of tertiary oil recovery, and in particular to a kind of side regulated and controled with endogenous microbes group of recovering the oil
Method.
Background technology
The endogenous microbes displacement of reservoir oil refers to using the microbiologic population's system formed during oil reservoir long-term injecting water, by note
The microbiologic population in activator activation oil reservoir, the surface reactive material generated using microorganism itself and metabolism are injected in well
It has an effect with biogas and the crude oil in oil reservoir, improves crude output and recovery ratio, be an environmental protection, inexpensive exploitation skill
Art.
Endogenous microbes are sequentially distributed aerobic microbiological, facultative aerobic microbiological in oil reservoir from water injection well to producing well
And anaerobe, different types of microorganism run through entire oil reservoir, different type microorganism and its metabolite jointly with oil
Crude oil effect in Tibetan, to generate oil displacement efficiency.Although but being noted from water injection well in endogenous microbes oil displacement process at present
Enter different types of microorganism activator activation aerobic microbiological, facultative aerobic microbiological and anaerobe, but it is different
The activator of type is to inject oil reservoir from water injection well, and there are Adsorption And Retention effects for one side activator itself, on the other hand not
There is overlapping in same type activator, influence effective activation of microorganism.Therefore, it is necessary to the micro- lifes of different type in clear oil reservoir
The regularity of distribution of object, and then realize the accurate injection of different type microorganism activator, realize effectively activation.
By literature search, notification number " CN104481476A ", a kind of patent name " microbial oil displacement raising oil recovery factor
Method ", disclosing a kind of method that microbial oil displacement improves oil recovery factor will be micro- by detecting endogenous microbes type
The biological displacement of reservoir oil has been divided into stages aerobic, facultative, that the anaerobe displacement of reservoir oil is different.But the disadvantages of this method is：(1) in oil reservoir
Different type microorganism include aerobic, facultative aerobic and anaerobe be it is simultaneous from water injection well to producing well, can not
The microorganism activation displacement of reservoir oil of a certain type can be only existed；(2) the different microorganisms displacement of reservoir oil stage is injected by water injection well in oil reservoir,
And it is not injected into the difference of speed and injection mode, activator is mainly consumed utilization in oil reservoir middle front part, is unfavorable for oil reservoir
The activation of medium and deep microorganism；(3) bacterium solution that injection different type microorganism is also needed in the above method, needs to micro- life
Object is screened, is cultivated, is fermented, and implementation process complexity is cumbersome, is unfavorable for field conduct, but also asking there are oil reservoir conformability
Topic.
Invention content
The purpose of the present invention is provide a kind of oil recovery endogenous microbes group tune in view of the above shortcomings of the prior art
The method of control.The present invention carries out the screening of experiment oil reservoir first, determines simulation core parameter according to the condition of experiment oil reservoir, in turn
The physical simulation experiment for carrying out experiment oil reservoir determines microbiologic population's distribution rule according to experiment reservoir physical simulation experimental result
Rule finally carries out field test to clear different type microorganism activator injection amount and live injection technology.The present invention
Can effectively different types of microorganism in activation experiment oil reservoir, simultaneously effective reduce the investment of microorganism activator at
This, to realize that the recovery ratio for increasing substantially experiment oil reservoir, field test results are apparent.
The invention discloses a kind of methods that oil recovery is regulated and controled with endogenous microbes group, which is characterized in that specifically include with
Lower step, but it is not limited to following steps：
(1) screening of oil reservoir is tested
It is necessary to meet following condition for the screening of experiment oil reservoir：90 DEG C of reservoir temperature <, reservoir pressure < 20MPa, permeability
> 100 × 10-3μm2, reservoir formation water salinity < 200000mg/L and Reservoir Crude Oil viscosity < 50000mPas.
(2) determination of experimental simulation core parameters
Experimental simulation core parameters include：Rock core length, diameter and sample point；
Experimental simulation rock core length：It is 100- that the distance between oil reservoir oil-water well, which is tested, with experimental simulation rock core length ratio
200:1；
Experimental simulation core diameter：It is 50-80 to test reservoir thickness and experimental simulation core diameter ratio:1；
Experimental simulation boring sample point：Sample point quantity is that a sample point is arranged at interval of 0.1m-0.2m, and sample point is equal
It is even to be distributed on experimental simulation rock core.
(3) physical simulation experiment of oil reservoir is tested
Using above-mentioned experimental simulation rock core carry out experiment oil reservoir physical simulation experiment, experiment the specific steps are：
Load experimental simulation rock core identical with experiment Reservoir Permeability；Simulation core vacuumizes, saturation testing oil reservoir
Water flooding；The dehydration degassed crude of saturation testing oil reservoir；Experimental simulation rock core is placed under experiment reservoir temperature and pressure condition
10d, then carries out a water drive, and a water drive is extremely aqueous consistent with experiment oil reservoir current composite；Experimental simulation boring sample point
It is sampled, each sample point sampling amount is 10-20mL；Aerobic, the facultative aerobic and anaerobe content of test sample.
(4) in experimental simulation rock core microbiologic population's regularity of distribution determination
According to above-mentioned test result, aerobic, facultative aerobic and anaerobe distribution rule in experimental simulation rock core are analyzed
Rule determines aerobic and facultative aerobic, the facultative aerobic separation with anaerobe.
(5) determination of microbiologic population's regularity of distribution in oil reservoir is tested
Aerobic and facultative aerobic, the facultative aerobic separation with anaerobe, the Yi Jishi determined according to above-mentioned steps
The ratio for testing the distance between oil reservoir oil-water well and experimental simulation rock core length, determine respectively experiment oil reservoir in it is aerobic with it is facultative
Aerobic separation and water injection well distance L1, facultative aerobic separation and water injection well distance L with anaerobe2。
(6) determination of activator injection amount
Activator includes aerobic microbiological activator, facultative aerobic microbiological activator and anaerobe activator.
(7) determination of activator scene injection technology
Activator scene injection technology be in the experiment oil reservoir determined according to above-mentioned steps it is aerobic with it is facultative aerobic and simultaneous
Facultative aerobic and anaerobe activator note is respectively set at aerobic position corresponding with the separation of anaerobe in property
Entrance, facultative aerobic and anaerobe activator are injected from above-mentioned inlet respectively, and aerobic microbiological activator is oily from experiment
It is injected in the water injection well of Tibetan.
(8) field test and effect assessment
Field test is carried out according to above-mentioned determining activator injection amount and activator scene injection technology, after the test
Statistics and the analysis for carrying out field test results, calculate the raising recovery ratio value and input-output ratio of experiment oil reservoir.
The aerobic microbiological includes bacillus subtilis, pseudomonas aeruginosa and acinetobacter calcoaceticus.
The facultative aerobic microbiological includes ground bacillus, beam hanger bacterium and achromobacter.
The anaerobe includes methanogen, nitrate reduction bacterium and anaerobism dialister bacterium.
The aerobic microbiological activator is starch 1.5-2.0wt%, peptone 0.9-1.2wt%, dipotassium hydrogen phosphate
0.3-0.6wt%, hydrogen peroxide 1.0-1.5wt%.
The facultative aerobic microbiological activator be glucose 1.8-2.2wt%, Dried Corn Steep Liquor Powder 1.0-1.4wt%,
Disodium hydrogen phosphate 0.4-0.8wt%.
The anaerobe activator is starch 1.5-2.0wt%, Dried Corn Steep Liquor Powder 0.5-0.8wt%, phosphoric acid hydrogen
Diamino 0.3-0.6wt%.
The determination of the aerobic microbiological activator injection amount is as follows：
V1=R2L1Фβ1
In formula：V1- aerobic microbiological activator injection amount, m3；
R-experiment reservoir thickness, m；
L1- aerobic at a distance from facultative aerobic separation and water injection well, m；
The porosity of Ф-experiment oil reservoir, dimensionless；
β1- use level coefficient, dimensionless, value range are 0.5~0.6.
The determination of the facultative aerobic microbiological activator injection amount is as follows：
V2=R2(L2-L1)Фβ2
In formula：V2- facultative aerobic microbiological activator injection amount, m3；
R-experiment reservoir thickness, m；
L1- aerobic at a distance from facultative aerobic separation and water injection well, m；
L2At a distance from-facultative aerobic separation and water injection well with anaerobe, m；
Ф-reservoir pore degree, dimensionless；
β2- use level coefficient, dimensionless, value range are 0.8~1.0.
The determination of the anaerobe activator injection amount is as follows：
V3=R2(L-L2)Фβ3
In formula：V3- anaerobe activator injection amount, m3；
R-experiment reservoir thickness, m；
L-experiment oil reservoir injection well is at a distance from oil well, m；
L2At a distance from-facultative aerobic separation and water injection well with anaerobe, m；
Ф-reservoir pore degree, dimensionless；
β3- use level coefficient, dimensionless, value range are 0.6~0.8.
The present invention has the following advantages that compared with prior art and advantageous effect：
(1) specify that different location is aerobic, facultative aerobic in experiment oil reservoir by simulated experiment using experimental simulation rock core
And the distributed areas of anaerobe, the design for activator injection amount provide foundation；
(2) activator that different type microorganism is injected from experiment oil reservoir different location, improves activator activating microorganisms
Specific aim and validity, to improve the oil displacement efficiency of microorganism, input-output ratio 1:12 or more, it is big to improve recovery ratio
In 20%；
(3) activator system is oriented activation according to distributed areas of the microorganism in testing oil reservoir, effectively prevents
The absorption and delay of activator component, save activator cost；
(4) specific aim and operability of the method field conduct are strong, the production low with cost of winning compared with conventional method
Go out the advantages that liquid is without subsequent processing, safety and environmental protection.
Specific implementation mode
With reference to specific embodiment, the present invention is described in further detail：
Embodiment 1：
Test block A overviews in Shengli Oil Field oil recovery factory：65 DEG C, reservoir pressure 11MPa of reservoir temperature, core intersection
6.5m, permeability 800 × 10-3μm2, formation water salinity 8500mg/L, porosity 26%, viscosity of crude 1200mPas is comprehensive
Aqueous 93.5% is closed, recovery percent of reserves 24.0%, average well spacing is 240m.Carry out micro- life in the oil reservoir using the method for the present invention
The object displacement of reservoir oil, is as follows：
(1) screening of oil reservoir is tested
Reservoir temperature is 65 DEG C, reservoir pressure 11MPa, Reservoir Permeability are 800 × 10-3μm2, formation water salinity be
8500mg/L, viscosity of crude 1200mPas.Meet the screening criteria of the experiment oil reservoir of the present invention, therefore this hair can be implemented
It is bright.
(2) determination of experimental simulation core parameters
Experimental simulation rock core length：The distance between oil-water well is 240m, the distance between experiment oil reservoir oil-water well and reality
It is 100 to test simulation core length ratio:1, the length for calculating rock core is 2.4m.
Experimental simulation core diameter：Reservoir thickness is 6.5m, tests reservoir thickness and experimental simulation core diameter ratio 50:
1, it is 0.13m to calculate core diameter.
Experimental simulation boring sample point：Rock core length is 2.4m, and 0.2m is divided between sample point, and design sample points are 12
It is a.
(3) physical simulation experiment of oil reservoir is tested
Using above-mentioned experimental simulation rock core carry out experiment oil reservoir physical simulation experiment, experiment the specific steps are：1. according to
Oil reservoir A conditions load rock core, and rock core basic demand is permeability 800 × 10-3μm2, porosity 26%, saturated pool water flooding,
Saturated pool is dehydrated degassed crude, and rock core is simulated oil reservoir aging at temperature and placed 10 days；2. water drive of rock core, a water drive extraction
Degree 24%, Produced Liquid aqueous 93.5%；3. carry out microbiological analysis is sampled to 12 sample points of rock core after a water drive,
Specific data are as shown in table 1 below：
The dense variation of different type microbial bacteria in 1 boring sample point production fluid of table
(4) in experimental simulation rock core microbiologic population's regularity of distribution determination
According to above-mentioned test result, rock core displacement test analysis result shows that aerobic microbiological is mainly distributed on the 1 of rock core
Region between number-No. 3 sample points, facultative aerobic microbiological are mainly distributed on the region between No. 4-No. 7 sample points of rock core, detest
Oxygen animalcule is mainly distributed on the region between No. 8-No. 12 sample points of rock core.So being advised according to the distribution of different zones microorganism
Rule determines aerobic and facultative aerobic microbiological separation apart from the position of core entry 0.6m, the facultative aerobic and micro- life of anaerobism
The separation of object is apart from the position of core entry 1.4m.
(5) determination of microbiologic population's regularity of distribution in oil reservoir is tested
Aerobic and facultative aerobic, the facultative aerobic separation with anaerobe, the Yi Jishi determined according to above-mentioned steps
Test the ratio 100 of the distance between oil reservoir oil-water well and experimental simulation rock core length:1, determine experiment oil reservoir in it is aerobic with it is simultaneous
Property aerobic separation and water injection well distance L1For 60m, facultative aerobic separation and water injection well with anaerobe away from
From L2For 140m.
(6) determination of activator injection amount
Activator includes aerobic microbiological activator, facultative aerobic microbiological activator and anaerobe activator.Its
Middle aerobic microbiological activator is：Starch 1.5wt%, peptone 1wt%, dipotassium hydrogen phosphate 0.3wt%, hydrogen peroxide 1.5wt%,
The determination of aerobic microbiological activator injection amount is as follows：
V1=3.14R2L1Фβ1=1035m3
In formula：V1- aerobic microbiological activator injection amount, m3；
R-experiment reservoir thickness 6.5m；
L1- injection well to the aerobic distance between facultative aerobic microbiological separation be 60m；
Ф-reservoir pore degree 0.26；
β1- use level coefficient, dimensionless, value 0.5.
Facultative aerobic microbiological activator is glucose 2.2wt%, Dried Corn Steep Liquor Powder 1.2wt%, disodium hydrogen phosphate
The determination of 0.6wt%, facultative aerobic microbiological activator injection amount are as follows：
V2=3.14R2(L2-L1)Фβ2=2483m3
In formula：V2- facultative aerobic microbiological activator injection amount, m3；
R-experiment reservoir thickness 6.5m；
L1- aerobic with facultative aerobic separation and water injection well distance 60m；
L2- facultative aerobic separation and water injection well distance 140m with anaerobe；
Ф-reservoir pore degree 0.26；
β2- use level coefficient, dimensionless, value 0.9.
Anaerobe activator is starch 1.6wt%, Dried Corn Steep Liquor Powder 0.7wt%, dihydrogen phosphate 0.5wt%, is detested
The determination of oxygen animalcule activator injection amount is as follows：
V3=3.14R2(L-L2)Фβ3=2424.5m3
In formula：V3- anaerobe activator injection amount, m3；
R-experiment reservoir thickness 6.5m；
L-experiment oil reservoir injection well and oil well distance 240m；
L2- facultative aerobic separation and water injection well distance 140m with anaerobe；
Ф-reservoir pore degree 0.26；
β3- use level coefficient, dimensionless, value 0.7.
(7) determination of activator injection technique
Activator scene injection technology be in the experiment oil reservoir determined according to above-mentioned steps it is aerobic with it is facultative aerobic and simultaneous
Facultative aerobic and anaerobe activator note is respectively set at aerobic position corresponding with the separation of anaerobe in property
Entrance, wherein aerobic microbiological activator are injected from the water injection well of experiment oil reservoir, and facultative aerobic microbiological activator is from distance
The inlet of water injection well 60m injects, and anaerobe activator is injected from the inlet apart from water injection well 140m.
(8) field test and effect assessment
Block comprehensive water cut drops to 76.5% by 93.5% after field test, aqueous to reduce by 17 percentage points, increasing
Produce crude oil 2.5 × 104T improves recovery ratio 21.8%, input-output ratio 1:10.5, field test results are good.
Embodiment 2
Test block B overviews in Shengli Oil Field oil recovery factory：80 DEG C, reservoir pressure 15MPa, core intersection 4m of reservoir temperature,
Permeability 1200 × 10-3μm2, formation water salinity 10500mg/L, porosity 28%, viscosity of crude 850mPas, synthesis contain
Water 94.8%, recovery percent of reserves 30%, average well spacing are 200m.Carry out endogenous microbes in the oil reservoir using the method for the present invention
The displacement of reservoir oil is as follows：
(1) screening of oil reservoir is tested
Reservoir temperature is 80 DEG C, reservoir pressure 15MPa, Reservoir Permeability are 1200 × 10-3μm2, formation water salinity
For 10500mg/L, viscosity of crude 850mPas.Meet the screening criteria of the experiment oil reservoir of the present invention, therefore this can be implemented
Invention.
(2) determination of experimental simulation core parameters
Experimental simulation rock core length：The distance between oil-water well is 200m, the distance between experiment oil reservoir oil-water well and reality
Test simulation core length ratio 200:1, it is 1m to calculate rock core length.
Experimental simulation core diameter：Reservoir thickness is 4m, tests reservoir thickness and experimental simulation core diameter ratio 50:1,
It is 0.08m to calculate core diameter.
Experimental simulation boring sample point：Rock core length is 2m, and 0.1m is divided between sample point, and it is 10 to calculate number of sampling
It is a.
(3) physical simulation experiment of oil reservoir is tested
Using above-mentioned experimental simulation rock core carry out experiment oil reservoir physical simulation experiment, experiment the specific steps are：1. according to
Oil reservoir B conditions load rock core, and rock core basic demand is permeability 1200 × 10-3μm2, porosity 28%, saturated pool water flooding,
Saturated pool is dehydrated degassed crude, and rock core is simulated reservoir temperature and placed 10 days；2. water drive of rock core, a water drive recovery percent of reserves
30%, Produced Liquid aqueous 94.8%；3. being sampled carry out microbiological analysis to 10 sample points of rock core after a water drive, specifically
Data are as shown in table 2 below：
The dense variation of different type microbial bacteria in 2 boring sample point production fluid of table
(4) in experimental simulation rock core microbiologic population's regularity of distribution determination
According to above-mentioned test result, rock core displacement test analysis result shows that aerobic microbiological is mainly distributed on the 1 of rock core
Region between number-No. 3 sample points, facultative aerobic microbiological are mainly distributed on the region between No. 4-No. 6 sample points of rock core, detest
Oxygen animalcule is mainly distributed on the region between No. 7-No. 10 sample points of rock core.So being advised according to the distribution of different zones microorganism
Rule determines aerobic and facultative aerobic microbiological separation apart from the position of core entry 0.3m, the facultative aerobic and micro- life of anaerobism
The separation of object is apart from the position of core entry 0.6m.
(5) determination of microbiologic population's regularity of distribution in oil reservoir is tested
Aerobic and facultative aerobic, the facultative aerobic separation with anaerobe, the Yi Jishi determined according to above-mentioned steps
The ratio for testing the distance between oil reservoir oil-water well and experimental simulation rock core length, determine experiment oil reservoir in it is aerobic with it is facultative aerobic
Separation and water injection well distance L1For 60m, facultative aerobic separation and water injection well distance L with anaerobe2For
120m。
(6) determination of activator injection amount
Activator includes aerobic microbiological activator, facultative aerobic microbiological activator and anaerobe activator.Its
Middle aerobic microbiological activator is：Starch 1.7wt%, peptone 1.1wt%, dipotassium hydrogen phosphate 0.4wt%, hydrogen peroxide
The determination of 1.3wt%, aerobic microbiological activator injection amount are as follows：
V1=3.14R2L1Фβ1=506m3
In formula：V1- aerobic microbiological activator injection amount, m3；
R-experiment reservoir thickness 4m；
L1- injection well to the aerobic distance between facultative aerobic microbiological separation be 60m；
Ф-reservoir pore degree 0.28；
β1- use level coefficient, dimensionless, value 0.6.
Facultative aerobic microbiological activator is glucose 1.9wt%, Dried Corn Steep Liquor Powder 1.3wt%, disodium hydrogen phosphate
The determination of 0.5wt%, facultative aerobic microbiological activator injection amount are as follows：
V2=3.14R2(L2-L1)Фβ2=759m3
In formula：V2- facultative aerobic microbiological activator injection amount, m3；
R-experiment reservoir thickness 4m；
L1- aerobic with facultative aerobic separation and water injection well distance 60m；
L2- facultative aerobic separation and water injection well distance 120m with anaerobe；
Ф-reservoir pore degree 0.28；
β2- use level coefficient, dimensionless, value 0.9.
Anaerobe activator is starch 1.8wt%, Dried Corn Steep Liquor Powder 0.8wt%, dihydrogen phosphate 0.6wt%, is detested
The determination of oxygen animalcule activator injection amount is as follows：
V3=3.14R2(L-L2)Фβ3=900m3
In formula：V3- anaerobe activator injection amount, m3；
R-experiment reservoir thickness 4m；
L-experiment oil reservoir injection well and oil well distance 200m；
L2- facultative aerobic separation and water injection well distance 120m with anaerobe；
Ф-reservoir pore degree 0.28；
β3- use level coefficient, dimensionless, value 0.8.
(7) determination of activator injection technique
Activator scene injection technology be in the experiment oil reservoir determined according to above-mentioned steps it is aerobic with it is facultative aerobic and simultaneous
Facultative aerobic and anaerobe activator note is respectively set at aerobic position corresponding with the separation of anaerobe in property
Entrance, wherein aerobic microbiological activator are injected from the water injection well of experiment oil reservoir, and facultative aerobic microbiological activator is from distance
The inlet of water injection well 60m injects, and anaerobe activator is injected from the inlet apart from water injection well 120m.
(8) statistics of field test results and analysis
Block comprehensive water cut drops to 84.5% by 94.8% after field test, aqueous to reduce by 10.3 percentage points,
Incremental oil production 3.2 × 104T improves recovery ratio 22.5%, input-output ratio 1:12.0, field test results are good.
Embodiment 3：
Test block C overviews in Shengli Oil Field oil recovery factory：75 DEG C, reservoir pressure 12.5MPa of reservoir temperature, core intersection
10m, permeability 1050 × 10-3μm2, formation water salinity 9400mg/L, porosity 32%, viscosity of crude 690mPas, synthesis
Aqueous 89%, recovery percent of reserves 24%, average well spacing is 300m.Carry out endogenous microbes in the oil reservoir using the method for the present invention
Oil displacement test is as follows：
(1) screening of oil reservoir is tested
Reservoir temperature is 75 DEG C, reservoir pressure 12.5MPa, Reservoir Permeability are 1050 × 10-3μm2, water flooding mineralising
Degree is 9400mg/L, viscosity of crude 690mPas.Meet the screening criteria of the experiment oil reservoir of the present invention, therefore can implement
The present invention.
(2) determination of experimental simulation core parameters
Experimental simulation rock core length：The distance between oil-water well is 300m, the distance between experiment oil reservoir oil-water well and reality
Test simulation core length ratio 100:1, calculate rock core length 3m.
Experimental simulation core diameter：Reservoir thickness is 10m, tests reservoir thickness and experimental simulation core diameter ratio 80:
1, it is 0.125m to calculate core diameter.
Experimental simulation boring sample point：Rock core length is 3m, and 0.2m is divided between sample point, and it is 15 to calculate number of sampling
It is a.
(3) physical simulation experiment of oil reservoir is tested
Using above-mentioned experimental simulation rock core carry out experiment oil reservoir physical simulation experiment, experiment the specific steps are：1. according to
Oil reservoir C conditions load rock core, and rock core basic demand is permeability 1050 × 10-3μm2, porosity 32%, saturated pool water flooding,
Saturated pool is dehydrated degassed crude, and rock core is simulated reservoir temperature and placed 10 days；2. water drive of rock core, a water drive recovery percent of reserves
24%, Produced Liquid aqueous 89%；3. being sampled carry out microbiological analysis to 15 sample points of rock core after a water drive, specific number
According to as shown in table 3 below：
The dense variation of different type microbial bacteria in 3 boring sample point production fluid of table
(4) in experimental simulation rock core microbiologic population's regularity of distribution determination
According to above-mentioned test result, rock core displacement test analysis result shows that aerobic microbiological is mainly distributed on the 1 of rock core
Region between number-No. 5 sample points, facultative aerobic microbiological are mainly distributed on the region between No. 6-No. 11 sample points of rock core,
Anaerobe is mainly distributed on the region between No. 12-No. 15 sample points of rock core.So according to point of different zones microorganism
Cloth rule determines the aerobic separation with facultative aerobic microbiological apart from the position of core entry 1m, facultative aerobic micro- with anaerobism
The separation of biology is apart from the position of core entry 2.2m.
(5) determination of microbiologic population's regularity of distribution in oil reservoir is tested
Aerobic and facultative aerobic, the facultative aerobic separation with anaerobe, the Yi Jishi determined according to above-mentioned steps
The ratio for testing the distance between oil reservoir oil-water well and experimental simulation rock core length, determine experiment oil reservoir in it is aerobic with it is facultative aerobic
Separation and water injection well distance L1For 100m, facultative aerobic separation and water injection well distance L with anaerobe2For
220m。
(6) determination of activator injection amount
Activator includes aerobic microbiological activator, facultative aerobic microbiological activator and anaerobe activator.Its
Middle aerobic microbiological activator is：Starch 1.8wt%, peptone 0.9wt%, dipotassium hydrogen phosphate 0.5wt%, hydrogen peroxide
The determination of 1.5wt%, aerobic microbiological activator injection amount are as follows：
V1=3.14R2L1Фβ1=5024m3
In formula：V1- aerobic microbiological activator injection amount, m3；
R-experiment reservoir thickness 10m；
L1- injection well to the aerobic distance between facultative aerobic microbiological separation be 100m；
Ф-reservoir pore degree 0.32；
β1- use level coefficient, dimensionless, value 0.5.
Facultative aerobic microbiological activator is glucose 2wt%, Dried Corn Steep Liquor Powder 1.3wt%, disodium hydrogen phosphate
The determination of 0.5wt%, facultative aerobic microbiological activator injection amount are as follows：
V2=3.14R2(L2-L1)Фβ2=9646m3
In formula：V2- facultative aerobic microbiological activator injection amount, m3；
R-experiment reservoir thickness 10m；
L1- aerobic with facultative aerobic separation and water injection well distance 100m；
L2- facultative aerobic separation and water injection well distance 220m with anaerobe；
Ф-reservoir pore degree 0.32；
β2- use level coefficient, dimensionless, value 0.8.
Anaerobe activator is starch 1.5wt%, Dried Corn Steep Liquor Powder 0.6wt%, dihydrogen phosphate 0.4wt%, is detested
The determination of oxygen animalcule activator injection amount is as follows：
V3=3.14R2(L-L2)Фβ3=4823m3
In formula：V3- anaerobe activator injection amount, m3；
R-experiment reservoir thickness 10m；
L-experiment oil reservoir injection well and oil well distance 300m；
L2- facultative aerobic separation and water injection well distance 220m with anaerobe；
Ф-reservoir pore degree 0.32；
β3- use level coefficient, dimensionless, value 0.6.
(7) determination of activator injection technique
Activator scene injection technology be in the experiment oil reservoir determined according to above-mentioned steps it is aerobic with it is facultative aerobic and simultaneous
Facultative aerobic and anaerobe activator note is respectively set at aerobic position corresponding with the separation of anaerobe in property
Entrance, wherein aerobic microbiological activator are injected from the water injection well of experiment oil reservoir, and facultative aerobic microbiological activator is from distance
The inlet of water injection well 100m injects, and anaerobe activator is injected from the inlet apart from water injection well 220m.
(8) statistics of field test results and analysis
Block comprehensive water cut drops to 74.5% by 89% after field test, aqueous to reduce by 14.5 percentage points, increasing
Produce crude oil 5.59 × 104T improves recovery ratio 21.7%, input-output ratio 1:13.2, field test results are good.
Claims (6)
1. a kind of method regulated and controled with endogenous microbes group of recovering the oil, which is characterized in that specifically include following steps, but be not limited to
Following steps：
(1) screening of oil reservoir is tested
It is necessary to meet following condition for the screening of experiment oil reservoir：90 DEG C of reservoir temperature <, reservoir pressure < 20MPa, permeability > 100
×10-3μm2, reservoir formation water salinity < 200000mg/L and Reservoir Crude Oil viscosity < 50000mPas；
(2) determination of experimental simulation core parameters
Experimental simulation core parameters include：Rock core length, diameter and sample point；
Experimental simulation rock core length：It is 100-200 that the distance between oil reservoir oil-water well, which is tested, with experimental simulation rock core length ratio:
1；Experimental simulation core diameter：It is 50-80 to test reservoir thickness and experimental simulation core diameter ratio:1；Experimental simulation rock core takes
Sampling point：Sample point quantity is that a sample point is arranged at interval of 0.1m-0.2m, and sample point is evenly distributed on experimental simulation rock core
On；
(3) physical simulation experiment of oil reservoir is tested
Using above-mentioned experimental simulation rock core carry out experiment oil reservoir physical simulation experiment, experiment the specific steps are：It loads and tests
The identical experimental simulation rock core of Reservoir Permeability；Simulation core vacuumizes, the water flooding of saturation testing oil reservoir；Saturation testing oil reservoir
Dehydration degassed crude；Experimental simulation rock core places 10d under experiment reservoir temperature and pressure condition, then carries out a water
It drives, a water drive is extremely aqueous consistent with experiment oil reservoir current composite；Experimental simulation boring sample point is sampled, each sample point
Sampling amount is 10-20mL；Aerobic, the facultative aerobic and anaerobe content of test sample；
(4) in experimental simulation rock core microbiologic population's regularity of distribution determination
According to above-mentioned test result, aerobic, facultative aerobic and anaerobe the regularity of distribution in experimental simulation rock core is analyzed, really
Set oxygen and facultative aerobic, the facultative aerobic separation with anaerobe；
(5) determination of microbiologic population's regularity of distribution in oil reservoir is tested
Aerobic and facultative aerobic, the facultative aerobic separation with anaerobe, and experiment oil determined according to above-mentioned steps
Hide the ratio of the distance between oil-water well with experimental simulation rock core length, determine respectively in experiment oil reservoir it is aerobic with it is facultative aerobic
Separation and water injection well distance L1, facultative aerobic separation and water injection well distance L with anaerobe2；
(6) determination of activator injection amount
Activator includes aerobic microbiological activator, facultative aerobic microbiological activator and anaerobe activator；
(7) determination of activator scene injection technology
Activator scene injection technology be in the experiment oil reservoir determined according to above-mentioned steps it is aerobic with it is facultative aerobic and facultative good
Facultative aerobic and anaerobe activator inlet is respectively set at oxygen position corresponding with the separation of anaerobe,
Facultative aerobic and anaerobe activator is injected from above-mentioned inlet respectively, note of the aerobic microbiological activator from experiment oil reservoir
It is injected in well；
(8) field test and effect assessment
Field test is carried out according to above-mentioned determining activator injection amount and activator scene injection technology, is carried out after the test
The statistics of field test results and analysis calculate the raising recovery ratio value and input-output ratio of experiment oil reservoir.
2. method according to claim 1 that regulated and controled with endogenous microbes group of recovering the oil, which is characterized in that described is aerobic
Microorganism includes bacillus subtilis, pseudomonas aeruginosa and acinetobacter calcoaceticus, and facultative aerobic microbiological includes ground bacillus, oil
Bacillus and achromobacter, anaerobe include methanogen, nitrate reduction bacterium and anaerobism dialister bacterium.
3. method according to claim 1 or 2 that regulated and controled with endogenous microbes group of recovering the oil, which is characterized in that described
Aerobic microbiological activator is starch 1.5-2.0wt%, peptone 0.9-1.2wt%, dipotassium hydrogen phosphate 0.3-0.6wt%, double
Oxygen water 1.0-1.5wt%, facultative aerobic microbiological activator are glucose 1.8-2.2wt%, Dried Corn Steep Liquor Powder 1.0-
1.4wt%, disodium hydrogen phosphate 0.4-0.8wt%, anaerobe activator are starch 1.5-2.0wt%, Dried Corn Steep Liquor Powder
0.5-0.8wt%, dihydrogen phosphate 0.3-0.6wt%.
4. method according to claim 1 that regulated and controled with endogenous microbes group of recovering the oil, which is characterized in that described is aerobic
The determination of microorganism activator injection amount is as follows：
V1=R2L1Фβ1
In formula：V1- aerobic microbiological activator injection amount, m3；
R-experiment reservoir thickness, m；
L1- aerobic at a distance from facultative aerobic separation and water injection well, m；
The porosity of Ф-experiment oil reservoir, dimensionless；
β1- use level coefficient, dimensionless, value range are 0.5~0.6.
5. method according to claim 1 or 4 that regulated and controled with endogenous microbes group of recovering the oil, which is characterized in that described
The determination of facultative aerobic microbiological activator injection amount is as follows：
V2=R2(L2-L1)Фβ2
In formula：V2- facultative aerobic microbiological activator injection amount, m3；
R-experiment reservoir thickness, m；
L1- aerobic at a distance from facultative aerobic separation and water injection well, m；
L2At a distance from-facultative aerobic separation and water injection well with anaerobe, m；
Ф-reservoir pore degree, dimensionless；
β2- use level coefficient, dimensionless, value range are 0.8~1.0.
6. method according to claim 5 that regulated and controled with endogenous microbes group of recovering the oil, which is characterized in that the anaerobism
The determination of microorganism activator injection amount is as follows：
V3=R2(L-L2)Фβ3
In formula：V3- anaerobe activator injection amount, m3；
R-experiment reservoir thickness, m；
L-experiment oil reservoir injection well is at a distance from oil well, m；
L2At a distance from-facultative aerobic separation and water injection well with anaerobe, m；
Ф-reservoir pore degree, dimensionless；
β3- use level coefficient, dimensionless, value range are 0.6~0.8.
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