CN108714238A - A kind of porous based composite dressing for medical use of fibroin/titanium and preparation method thereof of load Anti-radiation Microbes - Google Patents

A kind of porous based composite dressing for medical use of fibroin/titanium and preparation method thereof of load Anti-radiation Microbes Download PDF

Info

Publication number
CN108714238A
CN108714238A CN201810624336.3A CN201810624336A CN108714238A CN 108714238 A CN108714238 A CN 108714238A CN 201810624336 A CN201810624336 A CN 201810624336A CN 108714238 A CN108714238 A CN 108714238A
Authority
CN
China
Prior art keywords
fibroin
porous
titanium
radiation
medical use
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN201810624336.3A
Other languages
Chinese (zh)
Inventor
徐亚维
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dongguan Lianzhou Intellectual Property Operation and Management Co Ltd
Original Assignee
Dongguan Lianzhou Intellectual Property Operation and Management Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dongguan Lianzhou Intellectual Property Operation and Management Co Ltd filed Critical Dongguan Lianzhou Intellectual Property Operation and Management Co Ltd
Priority to CN201810624336.3A priority Critical patent/CN108714238A/en
Publication of CN108714238A publication Critical patent/CN108714238A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/22Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
    • A61L15/32Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/18Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing inorganic materials
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/40Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42Use of materials characterised by their function or physical properties
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42Use of materials characterised by their function or physical properties
    • A61L15/425Porous materials, e.g. foams or sponges
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42Use of materials characterised by their function or physical properties
    • A61L15/44Medicaments
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/412Tissue-regenerating or healing or proliferative agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2400/00Materials characterised by their function or physical properties
    • A61L2400/18Modification of implant surfaces in order to improve biocompatibility, cell growth, fixation of biomolecules, e.g. plasma treatment

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Materials Engineering (AREA)
  • Epidemiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Materials For Medical Uses (AREA)

Abstract

The present invention provides a kind of porous based composite dressing for medical use of fibroin/titanium and preparation method thereof of load Anti-radiation Microbes, includes the following steps:Using POROUS TITANIUM PLATE as backing material, thorough impregnation takes out in fibroin solutions through electric Gel Treatment, obtains the POROUS TITANIUM PLATE of fibroin gel cladding;The POROUS TITANIUM PLATE that fibroin gel coats is placed in -20~-80 DEG C of refrigerator after handling, it is composite porous to obtain fibroin/titanium for vacuum freeze-drying;It after the composite porous surface preparation of fibroin/titanium, is placed in Sabouraud dextrose enrichment liquid and cultivates 1d, be transferred to culture medium medium temperature chamber culture 1-7d, obtain the porous based composite dressing for medical use of fibroin/titanium of load Anti-radiation Microbes.Medical dressing prepared by the present invention is porous structure, and flexible, breathable moisturizing, high mechanical strength, good biocompatibility also has biological radiation resistance, comprehensive good, particularly suitable as surface of a wound medical dressing.

Description

It is a kind of load Anti-radiation Microbes the porous based composite dressing for medical use of fibroin/titanium and Preparation method
Technical field
The invention belongs to medical dressing field of material technology, and in particular to a kind of silk egg of load Anti-radiation Microbes Porous based composite dressing for medical use of in vain/titanium and preparation method thereof.
Background technology
X-ray is also referred to as roentgen-ray, is by the transition between two energy levels that energy differs greatly of the electronics in atom And the particle flux generated is excited, it is that there is very light penetration, electricity between gamma-rays and from a kind of electromagnetic wave between outer inspection From effect, fluorescence, heat effect and interference, diffraction reflection and refraction action, therefore X-ray has extensively in every field Application.But be chin while bringing convenient to our live and works also can harmful to human health, television tower, The electromagnetic radiation that the electrical equipments such as mobile phone, electromagnetic wave, launching tower, computer, television set, micro-wave oven generate is full of space, can It penetrates human body cell, destroy DNA, or even induce cancer cell, destroy red blood cell, it could even be possible to life security is threatened, therefore, It reduces various radiation intensity and the control of its dosage within safety line, achievees the purpose that ensure health safety.
Medical dressing be it is a kind of can temporary protection wound, the medical material that prevents wound infection, promote healing, it is current Medical dressing focus primarily upon research control wound fluid, avoid or control wound infection, removal or control wound stink, Mitigate pain, promote wound healing etc., but the wound for being the absence of crust protection is easier directly to be endangered by electromagnetic radiation Evil, it is easier to be compromised, therefore, it is very necessary study the medical dressing with radiation-resisting functional.Radiation resistance material is usually Ionising radiation is handled by way of absorbing or dissipating, and mainly has the anti-spoke of metallic fiber using relatively broad radiation resistance material Penetrate material and semimetal fiber radiation resistance material etc..A kind of functionality Medical face fabric disclosed in Chinese patent CN 104473365B And preparation method thereof, which includes internal layer, outer layer and the base being placed between internal layer and outer layer, and the wherein warp of internal layer is to wash Cotton blended fabric material, weft are terylene/alloy conductive yarn mixed yarn, and the warp of outer layer is chitosan fiber containing zinc, and weft is Imbue fibers, base are organosilicon/polyvinyl alcohol/graphene oxide composite membrane of porous structure, and internal layer and outer layer all use one On once plain weave, base be prepared by electrostatic spinning, internal layer, outer layer and link base layer are overpressurized heat treatment and bake system Standby to form, multifunctional medical fabric prepared by this method has stronger bacteria resistance, radioresistance, anti-static effect, and face Material is soft, moisture absorbing and sweat releasing effect is good, comfortable and easy to wear.A kind of Medical anti-radiation material disclosed in Chinese patent CN 106297928B, The radiation proof material is multilayered structure, including radiation protective layer, buffer layer and protective layer, and wherein radiation protective layer includes that cupro lead is thin The mixture of powder and coumarone indene resin fusing, buffer layer includes bamboo charcoal fiber, and protective layer includes containing super fine zinc oxide particle The preparation method of textile material, wherein radiation protective layer is:Solid coumarone resin is added in the metal can of preheating, is slowly risen Then cupro lead is added to melting in temperature, continue to stop heating after heating stirring, and stirring is to being uniformly mixed, after being cooled to room temperature Broken to obtain granular substance, as radiation protective layer, radiation proof material prepared by this method is mainly used for radioexmination and treatment Vest weaving and medical radioactive instrument security mask equipment in.By the above-mentioned prior art it is found that the doctor studied at present Radiation proof material is used to be difficult to more work(as collection breathable moisture permeability, biocompatibility, Antimicrobial preservative and radiation resistance one It can Medical wounded surface dressing.
Invention content
The technical problem to be solved in the present invention is to provide a kind of fibroin/titanium of load Anti-radiation Microbes is porous compound Medical dressing and preparation method thereof, the present invention are porous on the surface of POROUS TITANIUM PLATE and internal formation using POROUS TITANIUM PLATE as carrier Fibroin structure, then it is composite porous for substrate with fibroin/titanium, it cultivates to obtain the micro- life of radiation hardness on the surface of titanium plate Object further enhances the radiation resistance of medical dressing, obtains the Medical coating for integrating comfortable, high-strength pro-skin and radioresistance Material.
In order to solve the above technical problems, the technical scheme is that:
A kind of porous based composite dressing for medical use of fibroin/titanium of load Anti-radiation Microbes, the micro- life of the load radiation hardness The porous based composite dressing for medical use of fibroin/titanium of object includes porous silk albumen, POROUS TITANIUM PLATE and Anti-radiation Microbes, described more Hole silk is that fibroin is freeze-dried after electric Gel Treatment and obtains.
As a preferred embodiment of the above technical solution, the porous composite medical of fibroin/titanium of the load Anti-radiation Microbes Dressing further includes cotton gauze.
As a preferred embodiment of the above technical solution, the porous compound doctor of fibroin/titanium of the load Anti-radiation Microbes With the preparation method of dressing, include the following steps:
(1) using POROUS TITANIUM PLATE as backing material, thorough impregnation takes out in fibroin solutions through electric Gel Treatment, Obtain the POROUS TITANIUM PLATE of fibroin gel cladding;
(2) POROUS TITANIUM PLATE of fibroin gel cladding prepared by step (1) is placed in -20~-80 DEG C of refrigerator After reason, it is composite porous to obtain fibroin/titanium for vacuum freeze-drying;
(3) after the composite porous surface preparation of fibroin/titanium for preparing step (2), it is placed in Sabouraud dextrose 1d is cultivated in enrichment liquid, is transferred to culture medium medium temperature chamber culture 1-7d, and the fibroin/titanium for obtaining load Anti-radiation Microbes is more Hole based composite dressing for medical use.
As a preferred embodiment of the above technical solution, in the step (1), POROUS TITANIUM PLATE is prepared by the printing of 3D printing powder It arrives, the thickness of POROUS TITANIUM PLATE is 0.5-2mm, and porosity is more than 30%.
As a preferred embodiment of the above technical solution, in the step (1), the mass fraction of fibroin solutions is 4-6%.
As a preferred embodiment of the above technical solution, in the step (1), the voltage of coagulation glue is 15-30V, time 30- 90min。
As a preferred embodiment of the above technical solution, in the step (2), the time of vacuum freeze-drying is 2-3d, and vacuum degree is 0.01-0.05Pa。
As a preferred embodiment of the above technical solution, in the step (2), the composite porous middle silk egg of fibroin/titanium White and titanium mass ratio is 0.6-0.8:1, porosity 35-55%.
As a preferred embodiment of the above technical solution, in the step (3), the temperature of hot-house culture is 37 DEG C, and culture medium is fine jade Fat culture medium.
As a preferred embodiment of the above technical solution, it in the step (3), pre-processes as area load cotton gauze, it is described negative Load mode is chemical crosslinking or physical adherence.
Compared with prior art, the invention has the advantages that:
The porous based composite dressing for medical use of fibroin/titanium of load Anti-radiation Microbes prepared by the present invention includes porous silkworm POROUS TITANIUM PLATE prepared by three-dimensional printing technology is impregnated in fibroin solutions, makes by silk, POROUS TITANIUM PLATE and Anti-radiation Microbes Fibroin solutions are full of the inside and surface of POROUS TITANIUM PLATE, recycle electric gel technique, make inside POROUS TITANIUM PLATE and surface Fibroin forms gel, and composite porous, the porous composite wood is finally prepared using Vacuum Freezing & Drying Technology Porous silk albumen and POROUS TITANIUM PLATE, which interpenetrate, in material entwines, and forms dual network porous structure, not only contributes to microorganism Attachment and breeding, and ensure that the breathable moisture permeability of functional material, then again with composite porous for substrate, porous The surface of titanium plate is grown and microbial material of the breeding with radiation resistance, and the silk adhered to around POROUS TITANIUM PLATE, into one Step improves composite porous biocompatibility, further promotes the growth of Anti-radiation Microbes, is conducive to improve work( The radiation resistance of energy material.In addition the porous composite medical of fibroin/titanium for the load Anti-radiation Microbes that prepared by the present invention Dressing further includes cotton gauze, the cladding of cotton gauze does not influence other than containing composite porous conduct functional material The ventilative comfort of medical dressing also reduces external environment to composite porous influence, and prepared due to the present invention Contain sheet metal, therefore excellent in mechanical performance in porous based composite dressing for medical use, durability is good.
(2) preparation method of the invention is simple, is combined by coagulation glue, vacuum freeze drying and biological culture technigue, Porous metal material, multiporous biological protein material and microbial material are organically combined, mechanical property, gas permeability, parent is prepared The all good medical dressing of skin, radiation resistance is used particularly suitable as Medical wounded surface dressing, can be effectively promoted being cured for the surface of a wound It closes.
Specific implementation mode
Below in conjunction with specific embodiment, the present invention will be described in detail, herein illustrative examples and explanation of the invention For explaining the present invention, but it is not as a limitation of the invention.
Embodiment 1:
(1) it is 0.5mm by thickness, POROUS TITANIUM PLATE prepared by 3D printing powder printing of the porosity more than 30% is as support Material, thorough impregnation is in the fibroin solutions of 4wt%, and electric Gel Treatment 30min, takes out, obtain silkworm under the pressure of 15V The POROUS TITANIUM PLATE of silk-fibroin gel cladding.
(2) POROUS TITANIUM PLATE that fibroin gel coats is placed in -20 DEG C of refrigerator after handling, in 0.01P a vacuum Degree is lower to be lyophilized 2d, it is composite porous to obtain fibroin/titanium, wherein the composite porous middle fibroin of fibroin/titanium Mass ratio with titanium is 0.6:1, porosity 35%.
(3) it after area load cotton gauze, is placed in by way of chemical crosslinking by fibroin/titanium is composite porous 1d is cultivated in Sabouraud dextrose enrichment liquid, is transferred in agar medium, 1 d is cultivated at 37 DEG C, it is micro- to obtain load radiation hardness The porous based composite dressing for medical use of fibroin/titanium of biology.
Embodiment 2:
(1) it is 2mm by thickness, POROUS TITANIUM PLATE prepared by 3D printing powder printing of the porosity more than 30% is as fid Material, thorough impregnation is in the fibroin solutions of 6wt%, and electric Gel Treatment 90min, takes out, obtain silkworm under the pressure of 30V The POROUS TITANIUM PLATE of silk-fibroin gel cladding.
(2) POROUS TITANIUM PLATE that fibroin gel coats is placed in -80 DEG C of refrigerator after handling, in 0.05P a vacuum Degree is lower to be lyophilized 3d, it is composite porous to obtain fibroin/titanium, wherein the composite porous middle fibroin of fibroin/titanium Mass ratio with titanium is 0.8:1, porosity 35-55%.
(3) it after area load cotton gauze, is placed in by way of physical adherence by fibroin/titanium is composite porous 1d is cultivated in Sabouraud dextrose enrichment liquid, is transferred in agar medium, 7 d are cultivated at 37 DEG C, it is micro- to obtain load radiation hardness The porous based composite dressing for medical use of fibroin/titanium of biology.
Embodiment 3:
(1) it is 1mm by thickness, POROUS TITANIUM PLATE prepared by 3D printing powder printing of the porosity more than 30% is as fid Material, thorough impregnation is in the fibroin solutions of 4-6wt%, and electric Gel Treatment 60min, takes out, obtain silkworm under the pressure of 25V The POROUS TITANIUM PLATE of silk-fibroin gel cladding.
(2) POROUS TITANIUM PLATE that fibroin gel coats is placed in -60 DEG C of refrigerator after handling, in 0.03P a vacuum Degree is lower to be lyophilized 2.5d, it is composite porous to obtain fibroin/titanium, wherein the composite porous middle silk egg of fibroin/titanium White and titanium mass ratio is 0.7:1, porosity 45%.
(3) it after area load cotton gauze, is placed in by way of chemical crosslinking by fibroin/titanium is composite porous 1d is cultivated in Sabouraud dextrose enrichment liquid, is transferred in agar medium, 4 d are cultivated at 37 DEG C, it is micro- to obtain load radiation hardness The porous based composite dressing for medical use of fibroin/titanium of biology.
Embodiment 4:
(1) it is 1.5mm by thickness, POROUS TITANIUM PLATE prepared by 3D printing powder printing of the porosity more than 30% is as support Material, thorough impregnation is in the fibroin solutions of 4.5wt%, and electric Gel Treatment 45min, takes out, obtain under the pressure of 25V The POROUS TITANIUM PLATE of fibroin gel cladding.
(2) POROUS TITANIUM PLATE that fibroin gel coats is placed in -60 DEG C of refrigerator after handling, in 0.03P a vacuum Degree is lower to be lyophilized 2.5d, it is composite porous to obtain fibroin/titanium, wherein the composite porous middle silk egg of fibroin/titanium White and titanium mass ratio is 0.65:1, porosity 50%.
(3) it after area load cotton gauze, is placed in by way of chemical crosslinking by fibroin/titanium is composite porous 1d is cultivated in Sabouraud dextrose enrichment liquid, is transferred in agar medium, 5 d are cultivated at 37 DEG C, it is micro- to obtain load radiation hardness The porous based composite dressing for medical use of fibroin/titanium of biology.
Embodiment 5:
(1) it is 2mm by thickness, POROUS TITANIUM PLATE prepared by 3D printing powder printing of the porosity more than 30% is as fid Material, thorough impregnation is in the fibroin solutions of 5.5wt%, and electric Gel Treatment 75min, takes out, obtain silkworm under the pressure of 25V The POROUS TITANIUM PLATE of silk-fibroin gel cladding.
(2) POROUS TITANIUM PLATE that fibroin gel coats is placed in -50 DEG C of refrigerator after handling, in 0.02P a vacuum Degree is lower to be lyophilized 3d, it is composite porous to obtain fibroin/titanium, wherein the composite porous middle fibroin of fibroin/titanium Mass ratio with titanium is 0.7:1, porosity 40%.
(3) it after area load cotton gauze, is placed in by way of physical adherence by fibroin/titanium is composite porous 1d is cultivated in Sabouraud dextrose enrichment liquid, is transferred in agar medium, 6 d are cultivated at 37 DEG C, it is micro- to obtain load radiation hardness The porous based composite dressing for medical use of fibroin/titanium of biology.
Embodiment 6:
(1) it is 0.5mm by thickness, POROUS TITANIUM PLATE prepared by 3D printing powder printing of the porosity more than 30% is as support Material, thorough impregnation is in the fibroin solutions of 6wt%, and electric Gel Treatment 90min, takes out, obtain silkworm under the pressure of 15V The POROUS TITANIUM PLATE of silk-fibroin gel cladding.
(2) POROUS TITANIUM PLATE that fibroin gel coats is placed in -20 DEG C of refrigerator after handling, in 0.05P a vacuum Degree is lower to be lyophilized 2d, it is composite porous to obtain fibroin/titanium, wherein the composite porous middle fibroin of fibroin/titanium Mass ratio with titanium is 0.8:1, porosity 35%.
(3) it after area load cotton gauze, is placed in by way of physical adherence by fibroin/titanium is composite porous 1d is cultivated in Sabouraud dextrose enrichment liquid, is transferred in agar medium, 1 d is cultivated at 37 DEG C, it is micro- to obtain load radiation hardness The porous based composite dressing for medical use of fibroin/titanium of biology.
Embodiment 7:
(1) it is 2mm by thickness, POROUS TITANIUM PLATE prepared by 3D printing powder printing of the porosity more than 30% is as fid Material, thorough impregnation is in the fibroin solutions of 4wt%, and electric Gel Treatment 30min, takes out, obtain silkworm under the pressure of 30V The POROUS TITANIUM PLATE of silk-fibroin gel cladding.
(2) POROUS TITANIUM PLATE that fibroin gel coats is placed in -80 DEG C of refrigerator after handling, in 0.01P a vacuum Degree is lower to be lyophilized 3d, it is composite porous to obtain fibroin/titanium, wherein the composite porous middle fibroin of fibroin/titanium Mass ratio with titanium is 0.6:1, porosity 55%.
(3) it after area load cotton gauze, is placed in by way of chemical crosslinking by fibroin/titanium is composite porous 1d is cultivated in Sabouraud dextrose enrichment liquid, is transferred in agar medium, 7 d are cultivated at 37 DEG C, it is micro- to obtain load radiation hardness The porous based composite dressing for medical use of fibroin/titanium of biology.
Embodiment 8:
(1) it is 1.3mm by thickness, POROUS TITANIUM PLATE prepared by 3D printing powder printing of the porosity more than 30% is as support Material, thorough impregnation is in the fibroin solutions of 4-6wt%, and electric Gel Treatment 65min, takes out, obtain under the pressure of 23V The POROUS TITANIUM PLATE of fibroin gel cladding.
(2) POROUS TITANIUM PLATE that fibroin gel coats is placed in -75 DEG C of refrigerator after handling, in 0.04P a vacuum Degree is lower to be lyophilized 2-3d, it is composite porous to obtain fibroin/titanium, wherein the composite porous middle silk egg of fibroin/titanium White and titanium mass ratio is 0.75:1, porosity 45%.
(3) it after area load cotton gauze, is placed in by way of chemical crosslinking by fibroin/titanium is composite porous 1d is cultivated in Sabouraud dextrose enrichment liquid, is transferred in agar medium, 3. 5d are cultivated at 37 DEG C, obtains load radiation hardness The porous based composite dressing for medical use of fibroin/titanium of microorganism.
After testing, the porous based composite dressing for medical use of fibroin/titanium for the load Anti-radiation Microbes that prepared by embodiment 1-8 The result of gas permeability, mechanical property and radiation resistance is as follows:
As seen from the above table, the porous based composite dressing for medical use of fibroin/titanium for the load Anti-radiation Microbes that prepared by the present invention Good permeability, mechanical property and elasticity are excellent, also have good radiation resistance.
The above-described embodiments merely illustrate the principles and effects of the present invention, and is not intended to limit the present invention.It is any ripe The personage for knowing this technology can all carry out modifications and changes to above-described embodiment without violating the spirit and scope of the present invention.Cause This, institute is complete without departing from the spirit and technical ideas disclosed in the present invention by those of ordinary skill in the art such as At all equivalent modifications or change, should by the present invention claim be covered.

Claims (10)

1. a kind of porous based composite dressing for medical use of fibroin/titanium of load Anti-radiation Microbes, it is characterised in that:The load is resistance to The porous based composite dressing for medical use of fibroin/titanium for radiating microorganism includes porous silk albumen, POROUS TITANIUM PLATE and the micro- life of radiation hardness Object, the porous silk are that fibroin is freeze-dried after electric Gel Treatment and obtains.
2. a kind of porous based composite dressing for medical use of fibroin/titanium of load Anti-radiation Microbes according to claim 1, It is characterized in that:The porous based composite dressing for medical use of fibroin/titanium of the load Anti-radiation Microbes further includes cotton gauze.
3. the preparation of the porous based composite dressing for medical use of fibroin/titanium of load Anti-radiation Microbes described in claims 1 or 2 Method, which is characterized in that include the following steps:
(1) using POROUS TITANIUM PLATE as backing material, thorough impregnation takes out through electric Gel Treatment, obtains in fibroin solutions The POROUS TITANIUM PLATE of fibroin gel cladding;
(2) POROUS TITANIUM PLATE of fibroin gel cladding prepared by step (1) is placed in -20~-80 DEG C of refrigerator after handling, It is composite porous to obtain fibroin/titanium for vacuum freeze-drying;
(3) it after the composite porous surface preparation of fibroin/titanium for preparing step (2), is placed in Sabouraud dextrose and increases bacterium 1d is cultivated in liquid, is transferred to culture medium medium temperature chamber culture 1-7d, and the fibroin/titanium for obtaining load Anti-radiation Microbes is porous multiple Close medical dressing.
4. a kind of porous based composite dressing for medical use of fibroin/titanium of load Anti-radiation Microbes according to claim 3 Preparation method, it is characterised in that:In the step (1), POROUS TITANIUM PLATE is prepared by the printing of 3D printing powder, POROUS TITANIUM PLATE Thickness is 0.5-2mm, and porosity is more than 30%.
5. a kind of porous based composite dressing for medical use of fibroin/titanium of load Anti-radiation Microbes according to claim 3 Preparation method, it is characterised in that:In the step (1), the mass fraction of fibroin solutions is 4-6%.
6. a kind of porous based composite dressing for medical use of fibroin/titanium of load Anti-radiation Microbes according to claim 3 Preparation method, it is characterised in that:In the step (1), the voltage of coagulation glue is 15-30V, time 30-90min.
7. a kind of porous based composite dressing for medical use of fibroin/titanium of load Anti-radiation Microbes according to claim 3 Preparation method, it is characterised in that:In the step (2), the time of vacuum freeze-drying is 2-3d, vacuum degree 0.01-0.05Pa.
8. a kind of porous based composite dressing for medical use of fibroin/titanium of load Anti-radiation Microbes according to claim 3 Preparation method, it is characterised in that:In the step (2), the quality of fibroin/titanium composite porous middle fibroin and titanium Than for 0.6-0.8:1, porosity 35-55%.
9. a kind of porous based composite dressing for medical use of fibroin/titanium of load Anti-radiation Microbes according to claim 3 Preparation method, it is characterised in that:In the step (3), the temperature of hot-house culture is 37 DEG C, and culture medium is agar medium.
10. a kind of porous based composite dressing for medical use of fibroin/titanium of load Anti-radiation Microbes according to claim 3 Preparation method, it is characterised in that:It in the step (3), pre-processes as area load cotton gauze, the mode of loading is chemistry Crosslinking or physical adherence.
CN201810624336.3A 2018-06-16 2018-06-16 A kind of porous based composite dressing for medical use of fibroin/titanium and preparation method thereof of load Anti-radiation Microbes Withdrawn CN108714238A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810624336.3A CN108714238A (en) 2018-06-16 2018-06-16 A kind of porous based composite dressing for medical use of fibroin/titanium and preparation method thereof of load Anti-radiation Microbes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810624336.3A CN108714238A (en) 2018-06-16 2018-06-16 A kind of porous based composite dressing for medical use of fibroin/titanium and preparation method thereof of load Anti-radiation Microbes

Publications (1)

Publication Number Publication Date
CN108714238A true CN108714238A (en) 2018-10-30

Family

ID=63912175

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810624336.3A Withdrawn CN108714238A (en) 2018-06-16 2018-06-16 A kind of porous based composite dressing for medical use of fibroin/titanium and preparation method thereof of load Anti-radiation Microbes

Country Status (1)

Country Link
CN (1) CN108714238A (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103805797A (en) * 2014-01-17 2014-05-21 西北工业大学 Preparation method of medical porous titanium/porous titanium alloy
CN105664225A (en) * 2016-02-24 2016-06-15 苏州思彬纳米科技有限公司 Nano-silver, chitosan and fibroin compound biological dressing and preparation method thereof
CN105859294A (en) * 2016-03-31 2016-08-17 青岛百瑞吉生物工程有限公司 Method for preparing biomedical porous titanium-based composite material
CN107501961A (en) * 2017-09-17 2017-12-22 钱景 Titanium dioxide/Nano Silver/silk magnetic porous composite material and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103805797A (en) * 2014-01-17 2014-05-21 西北工业大学 Preparation method of medical porous titanium/porous titanium alloy
CN105664225A (en) * 2016-02-24 2016-06-15 苏州思彬纳米科技有限公司 Nano-silver, chitosan and fibroin compound biological dressing and preparation method thereof
CN105859294A (en) * 2016-03-31 2016-08-17 青岛百瑞吉生物工程有限公司 Method for preparing biomedical porous titanium-based composite material
CN107501961A (en) * 2017-09-17 2017-12-22 钱景 Titanium dioxide/Nano Silver/silk magnetic porous composite material and preparation method thereof

Similar Documents

Publication Publication Date Title
Xue et al. Bioinspired multifunctional biomaterials with hierarchical microstructure for wound dressing
Zhang et al. Nanocomposite membranes enhance bone regeneration through restoring physiological electric microenvironment
CA1212879A (en) Composite wound dressing
CN103768650A (en) Wound regenerating and repairing bandage and manufacture method thereof
CN104587515A (en) Medical wound dressing with anti-infection function
Guo et al. Facile and eco-friendly fabrication of biocompatible hydrogel containing CuS@ Ser NPs with mechanical flexibility and photothermal antibacterial activity to promote infected wound healing
CN108714238A (en) A kind of porous based composite dressing for medical use of fibroin/titanium and preparation method thereof of load Anti-radiation Microbes
Zheng et al. Waterproof and breathable wound dressing composited by expanded polytetrafluoroethylene backing and hydrogel
CN106474567B (en) A kind of flexibility artificial skin and preparation method
CN114470297B (en) Sugar-triggered response type wound quick-healing dressing and preparation method thereof
CN206579241U (en) A kind of glass facial mask
CN102328470A (en) Composite fabric
CN102584338B (en) Preparation method of composite biological coating on surface of carbon/carbon composite material
CN210596419U (en) Antibacterial moisture-absorbing and sweat-releasing fiber
CN115869453A (en) Double-layer antibacterial dressing loaded with antibacterial molecules, preparation and application
CN110256852A (en) The special type composite material and production technology and purposes of a kind of absorption of electromagnetic radiation
CN208085151U (en) A kind of anti-pilling thermal fabric
CN112263357A (en) Preparation method of medical artificial skin
CN111450315A (en) Inflammation-inhibiting hydrogel-coated polypropylene patch and preparation method thereof
CN213108506U (en) Novel antiviral fabric
CN216885639U (en) High temperature resistant high strength coated fabric
CN220535106U (en) Warm-keeping antibacterial fabric
Chen et al. Electrospun Multifunctional Radiation Shielding Nanofibrous Membrane for Daily Human Protection
CN114699552B (en) Preparation method and application of surface composite coating titanium mesh
CN215620501U (en) Far infrared heating and heat-preserving composite fabric

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WW01 Invention patent application withdrawn after publication
WW01 Invention patent application withdrawn after publication

Application publication date: 20181030