CN108707677A - The liquid-phase chip of sheep meat SNP marker and its composition - Google Patents
The liquid-phase chip of sheep meat SNP marker and its composition Download PDFInfo
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Abstract
The present invention relates to biotechnologys, mainly utilize SNP and liquid-phase chip technology.Using musculature sample as research object, to its data into obtaining 127 SNP labels sites after line program Quality Control.Design and synthesize the liquid-phase chip containing the label upstream and downstream primer.127 SNP site numbers are SMS001~SMS127.This 127 SNP labels applications will improve accuracy in detection, and shorten detection time, improve detection level, liquid-phase chip simultaneously containing this 127 SNP marker primer sequences can provide strong technical support and guarantee thus to discovery and medical diagnosis on disease, the identification of breeding and the generation of new varieties, the research of resistance and kind of the gene cloning of the important economical trait of domestic animal, the early prediction of hybrid vigour, marker assisted selection, novel drugs.
Description
Technical field
The liquid-phase chip of sheep meat SNP marker and its composition is related to molecular biosciences SNP technologies and chip field,
More particularly to the liquid-phase chip of SNP selection markers and its composition.
Background technology
In today's society, with the improvement of people ' s living standards with the enhancing of health care consciousness, to diet structure and nutrition
It is required that higher and higher.Especially to the intake of protein, and meat products is the main source of protein.Mutton has low fat, low
Cholesterol, high protein, meat tenderness succulence, unique flavor and it is easy to digest the features such as, be food and medicament dual-purpose, meat full of nutrition, more
Favored by consumers in general to get over, becomes universally acknowledged one of high-grade meat product, become main egg in human diet
One of white matter source has been shown to have good nutritive value.Its m eatquality and demand of the present consumer to nutrient meat
It is consistent, there is satisfactory edible quality.Mutton occupies part and parcel in China's meat products, and meat of a sheep is for we
Usually edible most mutton, it is mainly more slightly higher than chevon fat content because of meat of a sheep so that meat of a sheep is tasted more
Refinement is greasy palatable and very popular.
Identification is distinguished to meat by modern biotechnology means.Single nucleotide polymorphism（Single
Nucleotide Polymorphism, SNP）As in biological genome a kind of genetic molecule label, animals and plants heredity into
Change analysis, important economical trait screening, molecular breeding etc. and plays increasingly important role.Although new-generation sequencing technology
It flourishes, more and more species complete gene order-checking.But it relies on sequencing to carry out large-scale the high of SNP researchs to take
With threshold, or most of researcher is hung back.Chip with advantages such as its high performance-price ratio, data stabilizations, according to
The old mainstream market in occupation of SNP application studies.
From the point of view of current report, for the technology of SNP with being very extensive in terms of animal, Chen Xiao, Shi Wei etc. utilize SNP
Label carries out the discriminating of honeybee species；Beautiful Guo Yong of Qiu Li etc. carry out the identification of the kind of soybean using multiple SNP sites；Dong Yuan
Circle etc. finds that SNP typing methods are numerous, including digestion with restriction enzyme method, SNPshot, simpleness, PCR primer mispairing, burnt phosphorus
The certain methods such as sour sequencing, Invader, SNPlex and genetic chip, show SNP technologies be present illness genomics,
Pharmacogenomics and Swarm Evolution research etc. play significant role.Being used in some above-mentioned researchs for SNP obtains centainly
Achievement, but be even more a sky especially on SNP and liquid-phase chip technology in the meat of sheep or defective
In vain.
The indices of meat are detected using conventional method, testing cost is higher, time loss is long；And this
Invention is exactly the label using SNP and then analyzes meat, can be disposably meat by the information corresponding to SNP
Index is detected, and just as detection single amino acids, aliphatic acid, fast and accurately, testing cost substantially reduces, detection
Time also reduces accordingly, and then achievees the purpose that both quick and accurate true；In addition the identification of meat can also be carried out using these sites,
The type of meat can quickly be identified.
Liquid-phase chip is a kind of new technology that chip technology is combined with flow cytometry.DNA, antibody etc. are attached to
Microsphere surface is combined with determinand in the liquid phase as probe, the reporter molecule of fluorescent marker is added, by flow cytometer
Detect microsphere surface fluorescence marker.Liquid-phase chip is a kind of method of research and the detection for biological function, can be utilized
Some are attached to the primer sequence of microsphere surface, are combined with determinand, can quickly detect whether contain in determinand
There is the sequence being combined with known primer, to quickly judge to detect the meat of the meat.Meat includes to contain below
Amount:1. fat content:Studies have shown that the flavor of muscle is from fat, rather than from lean meat, Wasserman and Talley
(1968) research is pointed out, the flavor characteristic that the lean meat that all kinds of domestic animals are produced has its basic, and the formation master of its peculiar taste
If being determined by fat.Intramuscular fat content can improve the quality of mutton, in lean meat fat content increase can improve meat
The tenderness of product, intermuscular fat can improve the water holding capacity of meat；2. just moisture:Studies have shown that the first moisture of mutton contains
The succulence measured with meat has close relationship.The deliciousness and succulence of the height relationship mutton of first moisture；3. minerals
With micronutrient levels:Studies have shown that mineral element and trace element are the indispensable nutrients of livestock and poultry, to animal meat quality
There is important influence；4. amino acid:Amino acid is the base unit for constituting protein, studies have shown that amino acid kind in muscle
The influence of class and quantity to meat nutritive value and Meat Flavor is very big；5. aliphatic acid:Studies have shown that in muscle aliphatic acid kind
Class and composition are to determine adipose tissue physicochemical property.The important chemical composition for influencing meat flavor is evaluation meat nutriture value
It is worth one of the important indicator of height.
Based on above-mentioned factor, this invention is done ontology using Sheep Muscle and is studied, and has chosen longissimus dorsi muscle, rib respectively
Between 4 kinds of flesh, biceps muscle of thigh and lower limb flesh muscle samples, 127 SNP markers are had found on Sheep Muscle after program Quality Control
Site, and the gene and its primer sequence information that it is located at are found using these SNP sites, and then liquid-phase chip is made.It utilizes
The liquid-phase chip of these SNP sites provides scientific basis for the identification of later meat, contributes to the fat to muscle in this way
Content, first moisture, minerals and micronutrient levels, amino acid, aliphatic acid etc. carry out holistic approach.Additionally by online
DAVID softwares 127 genes corresponding to SNP are analyzed, and this time analysis includes GO（Gene Ontology）Function conspicuousness
Enrichment analysis and KEGG pathway（Kyoto Encyclopedia of Genes and Genomes）Conspicuousness enrichment point
Analysis.GO (Gene Ontology) is a standardized Gene Ontology, can describe comprehensively in organism gene and
The attribute of its product.Gene is pressed the bioprocess that gene participates in by GO（BP）, residing molecule cellular component (CC) and had
Three parts of function (MF) are classified, and are annotated to gene；KEGG is network analysis gene function, contact genome letter
The database of breath and functional information, contributing to researcher, network is analyzed as a whole by gene and expressing information.
The enrichment analysis of Pathway conspicuousnesses is examined by hypergeometry, finds the pathway of the difference expression gene of conspicuousness enrichment.It is right
Three classification, each is chosen preceding 10 gene clusters that P_value values are less than 0.05 and genetic enrichment number is most and is analyzed:
It was found that 127 genes are in bioprocess（BP）In be muscle systems develop；It is muscle fibre development in cellular component (CC)；
It is that dehydrogenase is related with the constituent of muscle in molecular function (MF), with carbon metablism and glycolysis is main side in KEGG
Formula.Clustering is carried out by the genotype to 127 SNP sites, four cluster groups are presented（Fig. 3）, and this four cluster groups
Corresponding is precisely four kinds of muscle samples of this carried out data analysis, can significantly distinguish the type of four kinds of muscle.
So also can be in terms of liquid genetic chip, Rapid identification goes out the type of muscle samples.
Invention content
The present invention provides the SNP sites of 127 identification sheep meats, it is characterised in that contains 127 SNP marker positions
The information of point, the branch's situation of site on chromosome are as shown in Figure 1.SNP site primer sequence corresponding to them and base
Because title is provided by the 5th of the case study on implementation in patent specification, 127 SNP site numbers are SMS001~SMS127.
The sheep meat SNP site, feature SMS001~SMS127 include the combination of SNP sequence informations,
And include the complementary series or homologous sequence of the sequence of SNP site.
The sheep meat SNP site, the DNA sequence dna primer structure corresponding to the sites feature SMS001~SMS127
At liquid-phase chip, corresponding carrier be liquid phase carrier.
Description of the drawings
Fig. 1 is the physical location schematic diagram that 127 SNP are located at 27 chromosomes of sheep.
Fig. 2 is PCR electrophoresis detection schematic diagrames.
Fig. 3 is PCA clustering figures.
Case study on implementation
The implementation of the present invention is mainly comprising SNP site screening and and preparation and application comprising site primer sequence, liquid-phase chip.
The sites 1.SNP determine
Utilize the muscle of the body different parts of sheep（Longissimus dorsi muscle, intercostal muscle, biceps muscle of thigh, lower limb flesh）Heavy sequencing data
With the file of the annotation of sheep, in conjunction with gene 50K length the characteristics of and rule, carry out Quality Control and selection, obtain 127 SNP
Marker site, the determination in these sites are also the basis that the making of following primer sequence and liquid-phase chip provides.
2. the synthesis of primer
127 pairs of primers are the sequence informations according to SMS001~SMS127 in subordinate list.
3. the preparation of liquid-phase chip
Primer sequence corresponding to above-mentioned 127 SMS001~SMS127 is carried out being mixed into liquid phase miniature droplets and is carried out a little
System.Liquid-phase chip is totally 96 orifice plate, sets up positive control and negative control wherein, to ensure the accuracy of experiment.
4. examples of implementation
The total serum IgE of muscle is extracted using Trizol methods and carries out resurveying sequence, then passes through the softwares such as samtools, VarScan
It is analyzed into line program, Quality Control goes out standard compliant SNP site and relevant information, details are listed by subordinate list.And utilize software
Primer5 is designed primer, establishes liquid-phase chip.PCR is built with this（Polymerase Chain Reaction）System,
Hybridized.The PCR total systems established are 20 microlitres of systems, including:CDNA is that 1 μ l, SMS001-F are 1 μ l, SMS001-R
For 1 μ l, Taq enzyme be 10 μ l, ddH2O it is 7 μ l.The reaction condition of PCR:95 DEG C of pre-degeneration 5min, 95 DEG C of denaturation 30S, 55 DEG C move back
Fiery 30S, 72 DEG C of extension 40S are recycled 32 times, last 72 DEG C of extensions 5min；The case where according to TM values, it is 13 to be divided on this basis
A standard, respectively 49 ° to 50 °, 50 ° to 51 °, 51 ° to 52 °, 52 ° to 53 °, 53 ° to 54 °, 54 ° to 55 °, 55 ° to 56 °,
56 ° to 57 °, 57 ° to 58 °, 58 ° to 59 °, 59 ° to 60 °, 60 ° to 61 °, 61 ° to 62 °；Wherein meet 49 ° to 50 ° of the positions SNP
Point has SMS026, SMS104, SMS003, SMS062, SMS093 totally 5,50 ° to 51 ° of SNP site have SMS013,
SMS029, SMS035, SMS027, SMS094, SMS018, SMS073 totally 7,51 ° to 52 ° of SNP site have SMS092,
SMS065、SMS054、SMS123、SMS053、SMS120、SMS102、SMS088、SMS096、SMS099、SMS087、SMS045、
SMS082 totally 13,52 ° to 53 ° of SNP site have SMS015, SMS098, SMS007, SMS002, SMS031, SMS057,
SMS030、SMS038、SMS050、SMS086、SMS127、SMS122、SMS052、SMS023、SMS001、SMS024、SMS046、
SMS064, SMS113 totally 19,53 ° to 54 ° of SNP site have SMS119, SMS008, SMS014, SMS020, SMS044,
SMS048, SMS005 totally 7,54 ° to 55 ° of SNP site have SMS060, SMS040, SMS010, SMS034, SMS090,
SMS078、SMS091、SMS085、SMS114、SMS069、SMS079、SMS058、SMS076、SMS022、SMS067、SMS043、
SMS077 totally 17,55 ° to 56 ° of SNP site have SMS004, SMS017, SMS080, SMS112, SMS125, SMS025,
SMS016, SMS028, SMS111, SMS019, SMS089, SMS124, SMS042, SMS072 totally 14,56 ° to 57 ° of the positions SNP
Point has SMS021, SMS012, SMS032, SMS118, SMS070, SMS061, SMS106, SMS041 totally 8,57 ° to 58 °
SNP site has SMS056, SMS011, SMS126, SMS068, SMS105, SMS033, SMS009, SMS055, SMS039 totally 9,
58 ° to 59 ° of SNP site have SMS109, SMS036, SMS121, SMS047, SMS051, SMS101, SMS117, SMS107,
SMS103, SMS059, SMS066 totally 11,59 ° to 60 ° of SNP site have SMS063, SMS075, SMS074, SMS006,
SMS097, SMS081, SMS108 totally 7,60 ° to 61 ° of SNP site have SMS115, SMS084, SMS071, SMS095,
SMS100, SMS037, SMS110 totally 7,61 ° to 62 ° of SNP site have SMS083, SMS049, SMS116 totally 3.PCR is produced
Object is detected using 1.5% agarose gel electrophoresis, such as the schematic diagram of Fig. 2.
5. the information of the sequence of primer and corresponding SNP site
SNP is numbered:SMS001；Gene Name:sheep_RPS8；Aligning primer left and right ends information is:Sense primer SEQ ID
NO:1:5'-GCCATCTGTATTTCCTTTATTC-3', downstream primer SEQ ID NO:2:5'-
CACTGTTATCAAACCCTTCTG-3'；
SMS001 reference informations:Positioned at 19229472 of No. 1 chromosome；Allele 1 is G, and allele 2 is A；Positioned at pair
It answers in the 5th exon of gene.
SNP is numbered:SMS002；Gene Name:sheep_PGM1；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:3:5'- TTTATGACTTTTGGTTGGAT -3', downstream primer SEQ ID NO:4:5'-
AGAATTTGTAGGCATGAGAT -3'；
SMS002 reference informations:Positioned at 38677500 of No. 1 chromosome；Allele 1 is T, and allele 2 is A；Positioned at pair
It answers in the 9th exon of gene.
SNP is numbered:SMS003；Gene Name:sheep_AGL；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:5:5'- ATTTATTAGGCCCCTGAGCT -3', downstream primer SEQ ID NO:6:5'-
TCTTAACACCCATATTTCGA -3'；
SMS003 reference informations:Positioned at 76462563 of No. 1 chromosome；Allele 1 is C, and allele 2 is A；Positioned at pair
It answers in the 31st exon of gene.
SNP is numbered:SMS004；Gene Name:sheep_PDE4DIP；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:7:5'- ACCAGGAGACCAGGTCAAAT -3', downstream primer SEQ ID NO:8: 5'-
TGGTGAAGGTCCATAAGTCC -3'；
SMS004 reference informations:Positioned at 96867241 of No. 1 chromosome；Allele 1 is A, and allele 2 is G；Positioned at pair
It answers in the 4th exon of gene.
SNP is numbered:SMS005；Gene Name:sheep_PDE4DIP；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:9:5'- ACTTCAGGGTTTCCTTCTCC -3', downstream primer SEQ ID NO:10: 5'-
GTAATCATTTCCAAGGGTCT -3'；
SMS005 reference informations:Positioned at 96920345 of No. 1 chromosome；Allele 1 is C, and allele 2 is T；Positioned at pair
It answers in the 18th exon of gene.
SNP is numbered:SMS006；Gene Name:sheep_CASQ1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:11:5'- TTGGCTCTGTCGGCAGTTTC -3', downstream primer SEQ ID NO:12: 5'-
TCTGGGTCGGCACGTCTCGT -3'；
SMS006 reference informations:Positioned at 109544995 of No. 1 chromosome；Allele 1 is T, and allele 2 is C；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS007；Gene Name:sheep_PPP1R2；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:13:5'- AGCTGCTATACAGTGAAGAA -3', downstream primer SEQ ID NO:14: 5'-
GTAACTTTGCCTGTGATTGA -3'；
SMS007 reference informations:Positioned at 190380609 of No. 1 chromosome；Allele 1 is G, and allele 2 is C；It is located at
In 6th exon of corresponding gene.
SNP is numbered:SMS008；Gene Name:sheep_NRD1；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:15:5'-ACAACAGCCTCATTCCC -3', downstream primer SEQ ID NO:16:5'-
CTCATTATTGACTACTTAGCG -3'；
SMS008 reference informations:Positioned at 25983145 of No. 1 chromosome；Allele 1 is G, and allele 2 is A；Positioned at pair
It answers in the 11st exon of gene.
SNP is numbered:SMS009；Gene Name:sheep_AMPD1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:17:5'- AAGCCTCACCGTCAATGTTC -3', downstream primer SEQ ID NO:18:5'-
CCAAGTCAAGTGGGAAGCAG -3'；
SMS009 reference informations:Positioned at 91207938 of No. 1 chromosome；Allele 1 is T, and allele 2 is C；Positioned at pair
It answers in the 13rd exon of gene.
SNP is numbered:SMS010；Gene Name:sheep_VDUP1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:19:5'- TCCAGGATGCCATTTCTACCC -3', downstream primer SEQ ID NO:20: 5'-
GCCAGCGAGATGATGTGATA -3'；
SMS010 reference informations:Positioned at 98337781 of No. 1 chromosome；Allele 1 is G, and allele 2 is A；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS011；Gene Name:sheep_TFRC；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:21:5'- TGGGACAAGGGATAGAGGAA -3', downstream primer SEQ ID NO:22: 5'-
TTGCCAATGAGGTCTGAAATAG -3'；
SMS011 reference informations:Positioned at 188913266 of No. 1 chromosome；Allele 1 is C, and allele 2 is T；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS012；Gene Name:sheep_FXR1；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:23:5'- TGGGTTGCCATCCCTTCTCC -3', downstream primer SEQ ID NO:24: 5'-
TTCAGTGGGTGGTGTCAGTA -3'；
SMS012 reference informations:Positioned at 204327898 of No. 1 chromosome；Allele 1 is T, and allele 2 is C；It is located at
In 12nd exon of corresponding gene.
SNP is numbered:SMS013；Gene Name:sheep_MLF1；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:25:5'- TCACAAAGAGGCTGAGCAGA -3', downstream primer SEQ ID NO:26: 5'-
ACACTCATCAGACTTTCCAT -3'；
SMS013 reference informations:Positioned at 226996845 of No. 1 chromosome；Allele 1 is G, and allele 2 is C；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS014；Gene Name:sheep_KIAA0368；Aligning primer left and right ends information is:Draw upstream
Object SEQ ID NO:27: 5'- GTGTATCTCAGGCATCAAGG -3', downstream primer SEQ ID NO:28: 5'-
TCTAAAGAGGGTTTCCCAAA -3'；
SMS014 reference informations:Positioned at 12044069 of No. 2 chromosome；Allele 1 is C, and allele 2 is T；Positioned at pair
It answers in the 33rd exon of gene.
SNP is numbered:SMS015；Gene Name:sheep_ZFAND5；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:29:5'- TTTTAGGATGTTTGGGTGTT -3', downstream primer SEQ ID NO:30: 5'-
TGTTTGGCACGAAGGAACTA -3'；
SMS015 reference informations:Positioned at 63738526 of No. 2 chromosome；Allele 1 is T, and allele 2 is C；Positioned at pair
It answers in the 5th exon of gene.
SNP is numbered:SMS016；Gene Name:sheep_BIN1；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:31:5'- GAACAATGACCTGCTCTGGATGG -3', downstream primer SEQ ID NO:32: 5'-
GCTCACCTTGATGTCAGGGAACT -3'；
SMS016 reference informations:Positioned at 117301256 of No. 2 chromosome；Allele 1 is A, and allele 2 is G；It is located at
In 5th exon of corresponding gene.
SNP is numbered:SMS017；Gene Name:sheep_TTN；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:33:5'- TAGAGGCTCACCGATTCATG -3', downstream primer SEQ ID NO:34: 5'-
CAAATAGTCCGACAAACACC -3'；
SMS017 reference informations:Positioned at 130196359 of No. 2 chromosome；Allele 1 is A, and allele 2 is C；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS018；Gene Name:sheep_TTN；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:35:5'- GCATCTTCGTGGCATGGGACA -3', downstream primer SEQ ID NO:36: 5'-
CAGTTGGTTTGCTGGGTGGG -3'；
SMS018 reference informations:Positioned at 130415194 of No. 2 chromosome；Allele 1 is A, and allele 2 is G；It is located at
In 74th exon of corresponding gene.
SNP is numbered:SMS019；Gene Name:sheep_NEB；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:37:5'- GCAGCCAAAGCCAACACCAA -3', downstream primer SEQ ID NO:38: 5'-
CAGCCACATCTAAAGACAGG -3'；
SMS019 reference informations:Positioned at 156679580 of No. 2 chromosome；Allele 1 is C, and allele 2 is T；It is located at
In 22nd exon of corresponding gene.
SNP is numbered:SMS020；Gene Name:sheep_NEB；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:39:5'- TCCAGTACGTTGGTCCATAA -3', downstream primer SEQ ID NO:40: 5'-
TCATCTCATCCCAGCCTTCA -3'；
SMS020 reference informations:Positioned at 156776689 of No. 2 chromosome；Allele 1 is A, and allele 2 is G；It is located at
In 78th exon of corresponding gene.
SNP is numbered:SMS021；Gene Name:sheep_KLHL41；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:41:5'- AGATTGCCTCCCAACCTACA -3', downstream primer SEQ ID NO:42: 5'-
CAAGATATTACAAGAACCCT -3'；
SMS021 reference informations:Positioned at 138842153 of No. 2 chromosome；Allele 1 is G, and allele 2 is A；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS022；Gene Name:sheep_XIRP2；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:43:5'- TGTCTCAGAAGAGGGCATTG -3', downstream primer SEQ ID NO:44: 5'-
CTCCCGGAGTTACTTGGTAT -3'；
SMS022 reference informations:Positioned at 141116728 of No. 2 chromosome；Allele 1 is T, and allele 2 is C；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS023；Gene Name:sheep_SF3B1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:45:5'- CATAGTAGCCAGTCCAGCAG -3', downstream primer SEQ ID NO:46: 5'-
CATGAGCATTGCATCAGTAA -3'；
SMS023 reference informations:Positioned at 198636469 of No. 2 chromosome；Allele 1 is G, and allele 2 is A；It is located at
In 13rd exon of corresponding gene.
SNP is numbered:SMS024；Gene Name:sheep_MYL1；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:47:5'- AGCCAACATGAAACTAGGAA -3', downstream primer SEQ ID NO:48: 5'-
GCATAAGGGGAGGATAAGAT -3'；
SMS024 reference informations:Positioned at 210945936 of No. 2 chromosome；Allele 1 is G, and allele 2 is A；It is located at
In 7th exon of corresponding gene.
SNP is numbered:SMS025；Gene Name:sheep_TUBA4A；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:49:5'- GATCAGCCTCCGTTCTTTAGC -3', downstream primer SEQ ID NO:50: 5'-
ACGACTCCTTCACCACCTTT -3'；
SMS025 reference informations:Positioned at 220177234 of No. 2 chromosome；Allele 1 is G, and allele 2 is A；It is located at
In 3rd exon of corresponding gene.
SNP is numbered:SMS026；Gene Name:sheep_HADHB；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:51:5'- TTTCCCTCTATTGCTATTACTTCC -3', downstream primer SEQ ID NO:52: 5'-
CTGTGACCTACCTGCCCTCC -3'；
SMS026 reference informations:Positioned at 33364292 of No. 3 chromosome；Allele 1 is C, and allele 2 is G；Positioned at pair
It answers in the 14th exon of gene.
SNP is numbered:SMS027；Gene Name:sheep_RMND5A；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:53:5'- CATCATCTTTGGGAACACTA -3', downstream primer SEQ ID NO:54: 5'-
AACCTGCCCTAAAACTAACT -3'；
SMS027 reference informations:Positioned at 58463973 of No. 3 chromosome；Allele 1 is A, and allele 2 is G；Positioned at pair
It answers in the 9th exon of gene.
SNP is numbered:SMS028；Gene Name:sheep_RTN4；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:55:5'- AATGTCAGTGCCAGTTTCCA -3', downstream primer SEQ ID NO:56: 5'-
TACTTAGCCACGGCATCTTT -3'；
SMS028 reference informations:Positioned at 68943495 of No. 3 chromosome；Allele 1 is G, and allele 2 is A；Positioned at pair
It answers in the 6th exon of gene.
SNP is numbered:SMS029；Gene Name:sheep_SLC38A2；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:57:5'- TGCTTCATACCTACTCTTCC -3', downstream primer SEQ ID NO:58: 5'-
ATCTTCATGCCTAACAACTC -3'；
SMS029 reference informations:Positioned at 139944734 of No. 3 chromosome；Allele 1 is C, and allele 2 is T；It is located at
In 6th exon of corresponding gene.
SNP is numbered:SMS030；Gene Name:sheep_MYBPC1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:59:5'- TTGAGTTCCAGCATCCTTGT -3', downstream primer SEQ ID NO:60: 5'-
AATCTTACCCTCTTTCTTAGTG -3'；
SMS030 reference informations:Positioned at 170450578 of No. 3 chromosome；Allele 1 is A, and allele 2 is G；It is located at
In the exon 2 of corresponding gene.
SNP is numbered:SMS031；Gene Name:sheep_MYBPC1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:61:5'- CAGAACTGATCGAAAAGACC -3', downstream primer SEQ ID NO:62: 5'-
TCAAAGGAGTGACAACAAGAG -3'；
SMS031 reference informations:Positioned at 170523344 of No. 3 chromosome；Allele 1 is G, and allele 2 is T；It is located at
In 18th exon of corresponding gene.
SNP is numbered:SMS032；Gene Name:sheep_YBX3；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:63:5'- AGACCACGACCTTACGAACA -3', downstream primer SEQ ID NO:64: 5'-
ATAAACCTAGTGGCTGCTGA -3'；
SMS032 reference informations:Positioned at 203781385 of No. 3 chromosome；Allele 1 is A, and allele 2 is T；It is located at
In 9th exon of corresponding gene.
SNP is numbered:SMS033；Gene Name:sheep_ATF4；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:65:5'- AAACCTTACGACCCTCCTGG -3', downstream primer SEQ ID NO:66: 5'-
CCCTTGCTTTGCGAACCTCT -3'；
SMS033 reference informations:Positioned at 214931488 of No. 3 chromosome；Allele 1 is C, and allele 2 is T；It is located at
In 3rd exon of corresponding gene.
SNP is numbered:SMS034；Gene Name:sheep_RHOQ；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:67:5'- TCTTGTGGTTTGGTCAGCAT -3', downstream primer SEQ ID NO:68: 5'-
CGTTCAACTGTAACAAGGCT -3'；
SMS034 reference informations:Positioned at 77673397 of No. 3 chromosome；Allele 1 is T, and allele 2 is C；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS035；Gene Name:sheep_TMEM182；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:69:5'- TTCACAAGCCAGGGGTCGGG -3', downstream primer SEQ ID NO:70: 5'-
AGTGGAATGTCTATTTCTTCTCAG -3'；
SMS035 reference informations:Positioned at 98974533 of No. 3 chromosome；Allele 1 is G, and allele 2 is T；Positioned at pair
It answers in the 5th exon of gene.
SNP is numbered:SMS036；Gene Name:sheep_RPL31；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:71:5'- GCCCAGACGGCTTTGTTGAG -3', downstream primer SEQ ID NO:72: 5'-
TGACGTGGTGGTGAAGGATT -3'；
SMS036 reference informations:Positioned at 100401365 of No. 3 chromosome；Allele 1 is C, and allele 2 is T；It is located at
In 3rd exon of corresponding gene.
SNP is numbered:SMS037；Gene Name:sheep_SMARCD1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:73:5'- TGCTCACCAAGCCTCCCTCC -3', downstream primer SEQ ID NO:74: 5'-
CACCATGACCGGCAAGAAAA -3'；
SMS037 reference informations:Positioned at 136038983 of No. 3 chromosome；Allele 1 is A, and allele 2 is G；It is located at
In 9th exon of corresponding gene.
SNP is numbered:SMS038；Gene Name:sheep_TMBIM6；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:75:5'- ATCATTCTTGGCACTGTTTC -3', downstream primer SEQ ID NO:76: 5'-
GATTTCCTTCCTATCAACCC -3'；
SMS038 reference informations:Positioned at 136357629 of No. 3 chromosome；Allele 1 is G, and allele 2 is C；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS039；Gene Name:sheep_PFKM；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:77:5'- ATCAGCTCTGTCCTTGCCTTAA -3', downstream primer SEQ ID NO:78: 5'-
GTCTCATCCCTGACCCTTTCT -3'；
SMS039 reference informations:Positioned at 138241428 of No. 3 chromosome；Allele 1 is C, and allele 2 is A；It is located at
In 7th exon of corresponding gene.
SNP is numbered:SMS040；Gene Name:sheep_MB；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:79:5'- TCCACCTCAGATCATCAGTTA -3', downstream primer SEQ ID NO:80: 5'-
GCACCTTTACCCTCACCTTG -3'；
SMS040 reference informations:Positioned at 178866631 of No. 3 chromosome；Allele 1 is A, and allele 2 is G；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS041；Gene Name:sheep_TPI1；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:81:5'- TCTCAGCCAAGAAGAAGCCACC -3', downstream primer SEQ ID NO:82: 5'-
CGACATCATCAATGCCAAACAATA -3'；
SMS041 reference informations:Positioned at 207527837 of No. 3 chromosome；Allele 1 is G, and allele 2 is A；It is located at
In the exon 2 of corresponding gene.
SNP is numbered:SMS042；Gene Name:sheep_DLD；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:83:5'- TCTTCCCTTTCAGACCTTAT -3', downstream primer SEQ ID NO:84: 5'-
TTCAATTCCGTCCAAACCTA -3'；
SMS042 reference informations:Positioned at 48976918 of No. 4 chromosome；Allele 1 is C, and allele 2 is T；Positioned at pair
It answers in the 14th exon of gene.
SNP is numbered:SMS043；Gene Name:sheep_PPP1R3A；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:85:5'- CCTTTGTCTTCAAGTTCATC -3', downstream primer SEQ ID NO:86: 5'-
TCTGCCATTAGTTGGTCTGC -3'；
SMS043 reference informations:Positioned at 54571140 of No. 4 chromosome；Allele 1 is G, and allele 2 is A；Positioned at pair
It answers in the 4th exon of gene.
SNP is numbered:SMS044；Gene Name:sheep_PDK4；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:87:5'- CCACGGAAAGAAGCCATACT -3', downstream primer SEQ ID NO:88: 5'-
TAATGCCTGAAAGCTGTAAT -3'；
SMS044 reference informations:Positioned at 12768698 of No. 4 chromosome；Allele 1 is G, and allele 2 is A；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS045；Gene Name:sheep_NDUFA4；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:89:5'- AGTCTGGACCTTCTTTCTTC -3', downstream primer SEQ ID NO:90: 5'-
TTTTCCAAGTTCTACTCTGT -3'；
SMS045 reference informations:Positioned at 19024317 of No. 4 chromosome；Allele 1 is T, and allele 2 is A；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS046；Gene Name:sheep_CAPZA2；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:91:5'- TCCACCTCAGATCATCAGTTA -3', downstream primer SEQ ID NO:92: 5'-
GCACCTTTACCCTCACCTTG -3'；
SMS046 reference informations:Positioned at 51428699 of No. 4 chromosome；Allele 1 is T, and allele 2 is C；Positioned at pair
It answers in the 7th exon of gene.
SNP is numbered:SMS047；Gene Name:sheep_PKD2L2；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:93:5'- CCTCTGCTTTCCCTGCTTCT -3', downstream primer SEQ ID NO:94: 5'-
TAACCCATCCCTCTTCTCCC -3'；
SMS047 reference informations:Positioned at 46724707 of No. 5 chromosome；Allele 1 is T, and allele 2 is C；Positioned at pair
It answers in the exon 2 of gene.
SNP is numbered:SMS048；Gene Name:sheep_CCNG1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:95:5'- TCCACCTCAGATCATCAGTTA -3', downstream primer SEQ ID NO:96: 5'-
GCACCTTTACCCTCACCTTG -3'；
SMS048 reference informations:Positioned at 72441870 of No. 5 chromosome；Allele 1 is T, and allele 2 is A；Positioned at pair
It answers in the 7th exon of gene.
SNP is numbered:SMS049；Gene Name:sheep_CKMT2；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:97:5'- NNNCAGTCCTTGGCTGTTCG -3', downstream primer SEQ ID NO:98: 5'-
TCTCAAGAGTTACCACGAAT -3'；
SMS049 reference informations:Positioned at 78568608 of No. 5 chromosome；Allele 1 is A, and allele 2 is C；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS050；Gene Name:sheep_SQSTM1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:99:5'- TGGTTATGAAATAGGCAGTA -3', downstream primer SEQ ID NO:100: 5'-
ATTATACCAGCAGTCCAGAG -3'；
SMS050 reference informations:Positioned at 1119808 of No. 5 chromosome；Allele 1 is C, and allele 2 is T；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS051；Gene Name:sheep_GNB2L1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:101:5'- AGTCCCGCCAAAGGCAATCA -3', downstream primer SEQ ID NO:102: 5'-
GTACCAGCAGTAAGGCAGAG -3'；
SMS051 reference informations:Positioned at 37888078 of No. 5 chromosome；Allele 1 is G, and allele 2 is A；Positioned at pair
It answers in the exon 2 of gene.
SNP is numbered:SMS052；Gene Name:sheep_MYOZ2；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:103:5'- GCTTTGCCAGTGAGTAGAAT -3', downstream primer SEQ ID NO:104: 5'-
TCATGGCCTTTACCAATCCT -3'；
SMS052 reference informations:Positioned at 6265267 of No. 6 chromosome；Allele 1 is G, and allele 2 is T；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS053；Gene Name:sheep_SYNPO2；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:105:5'- TTGATAGGTTCCTCCTTGAT -3', downstream primer SEQ ID NO:106: 5'-
CAGGCTTCTTGCCAGATGTT -3'；
SMS053 reference informations:Positioned at 6436138 of No. 6 chromosome；Allele 1 is G, and allele 2 is T；Positioned at pair
It answers in the exon 2 of gene.
SNP is numbered:SMS054；Gene Name:sheep_RPL9；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:107:5'- CAGGAATGCCTATTGGACAC -3', downstream primer SEQ ID NO:108: 5'-
TTCGCACAATCTGTAGTCACGT -3'；
SMS054 reference informations:Positioned at 58540327 of No. 6 chromosome；Allele 1 is G, and allele 2 is A；Positioned at pair
It answers in the 4th exon of gene.
SNP is numbered:SMS055；Gene Name:sheep_PAPD4；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:109:5'- ATCACAGCTCAGCCCTCCTC -3', downstream primer SEQ ID NO:110: 5'-
CTTCCGTCTTCACTTCATCC -3'；
SMS055 reference informations:Positioned at 10475144 of No. 7 chromosome；Allele 1 is C, and allele 2 is T；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS056；Gene Name:sheep_THBS4；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:111:5'- TTGCAGACACCATCCCAGAG -3', downstream primer SEQ ID NO:112: 5'-
CCCGTCAAAGCCTGAGCCAC -3'；
SMS056 reference informations:Positioned at 10811662 of No. 7 chromosome；Allele 1 is G, and allele 2 is A；Positioned at pair
It answers in the 21st exon of gene.
SNP is numbered:SMS057；Gene Name:sheep_RPLP1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:113:5'- TTTTCGGTGCTACCACAGGC -3', downstream primer SEQ ID NO:114: 5'-
ATCTCCACCCTCCTCACTCC -3'；
SMS057 reference informations:Positioned at 16136504 of No. 7 chromosome；Allele 1 is T, and allele 2 is C；Positioned at pair
It answers in the 3rd exon of gene.
SNP is numbered:SMS058；Gene Name:sheep_CAPN3；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:115:5'- TCCATGAAAGCAGGAGTGTA -3', downstream primer SEQ ID NO:116: 5'-
GACAGCAGAAGCCAAGAAAA -3'；
SMS058 reference informations:Positioned at 34803754 of No. 7 chromosome；Allele 1 is A, and allele 2 is T；Positioned at pair
It answers in the 24th exon of gene.
SNP is numbered:SMS059；Gene Name:sheep_PKM；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:117:5'- AGTCTTAACAGGAGCACTTGCC -3', downstream primer SEQ ID NO:118: 5'-
CCTCAGCCTCCCTCACCTTG -3'；
SMS059 reference informations:Positioned at 18782043 of No. 7 chromosome；Allele 1 is C, and allele 2 is T；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS060；Gene Name:sheep_TPM1；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:119:5'- TTGTGGTTTTATTCAGCGTT -3'Downstream primer SEQ ID NO:120: 5'-
TTCTTTGCTTCACTTCTCCC -3'；
SMS060 reference informations:Positioned at 44024206 of No. 7 chromosome；Allele 1 is A, and allele 2 is T；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS061；Gene Name:sheep_TRDN；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:121:5'- TCTTAGGTGCCACATACATT -3', downstream primer SEQ ID NO:122: 5'-
TCGATGAGGCAGAACCAACC -3'；
SMS061 reference informations:Positioned at 15087537 of No. 8 chromosome；Allele 1 is T, and allele 2 is C；Positioned at pair
It answers in the 20th exon of gene.
SNP is numbered:SMS062；Gene Name:sheep_TRDN；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:123:5'- TCCACCTCAGATCATCAGTTA -3', downstream primer SEQ ID NO:124: 5'-
GCACCTTTACCCTCACCTTG -3'；
SMS062 reference informations:Positioned at 15176038 of No. 8 chromosome；Allele 1 is G, and allele 2 is A；Positioned at pair
It answers in the 34th exon of gene.
SNP is numbered:SMS063；Gene Name:sheep_EEF1A1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:125:5'- AAGCTGGAAGATGGCCCTAA -3', downstream primer SEQ ID NO:126: 5'-
TGATGACACCCACAGCGACT -3'；
SMS063 reference informations:Positioned at 83012 of No. 8 chromosome；Allele 1 is G, and allele 2 is A；Positioned at correspondence
In the exon 2 of gene.
SNP is numbered:SMS064；Gene Name:sheep_PLN；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:127:5'- GTAGATTAGTATTGCTGGTT -3', downstream primer SEQ ID NO:128: 5'-
TTTATCCCACTACTGTTCAT -3'；
SMS064 reference informations:Positioned at 19474890 of No. 8 chromosome；Allele 1 is G, and allele 2 is C；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS065；Gene Name:sheep_TXLNB；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:129:5'- TACATGGGTTCTCAAATAGC -3', downstream primer SEQ ID NO:130: 5'-
AGAAAGGGAGGAAGAGGAAA -3'；
SMS065 reference informations:Positioned at 63935302 of No. 8 chromosome；Allele 1 is G, and allele 2 is A；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS066；Gene Name:sheep_ABRA；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:131:5'- AAGTGGGCAATCTGAAAGGG -3', downstream primer SEQ ID NO:132: 5'-
ATCGAGCGGATGATGAAACA -3'；
SMS066 reference informations:Positioned at 70496280 of No. 9 chromosome；Allele 1 is C, and allele 2 is T；Positioned at pair
It answers in the 3rd exon of gene.
SNP is numbered:SMS067；Gene Name:sheep_JPH1；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:133:5'- GGGTTAGGAAGGAGCAGTCA -3', downstream primer SEQ ID NO:134: 5'-
CCTTATTTGCTACCATTTCG -3'；
SMS067 reference informations:Positioned at 50346640 of No. 9 chromosome；Allele 1 is T, and allele 2 is A；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS068；Gene Name:sheep_CA3；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:135:5'- AGAATGAGTCCCTTCAGTTA -3', downstream primer SEQ ID NO:136: 5'-
CGTGAGGGTGGGATTAACAA -3'；
SMS068 reference informations:Positioned at 89664858 of No. 9 chromosome；Allele 1 is T, and allele 2 is C；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS069；Gene Name:sheep_SUCLA2；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:137:5'- TTTGATGCCTTCTACGATAT -3', downstream primer SEQ ID NO:138: 5'-
TTCTGTTTCCCTTCCTGCTA -3'；
SMS069 reference informations:Positioned at 18147113 of No. 10 chromosome；Allele 1 is G, and allele 2 is A；It is located at
In 7th exon of corresponding gene.
SNP is numbered:SMS070；Gene Name:sheep_RPL21；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:139:5'- TAAACTGCTCCCAGAACCAA -3', downstream primer SEQ ID NO:140: 5'-
TTAGAGGGAAACCCGTGAAA -3'；
SMS070 reference informations:Positioned at 32843929 of No. 10 chromosome；Allele 1 is T, and allele 2 is C；It is located at
In the exon 2 of corresponding gene.
SNP is numbered:SMS071；Gene Name:sheep_PPP1R27；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:141:5'- TTGGTGGAGCTATTCAAAGGG -3', downstream primer SEQ ID NO:142: 5'-
CGAAGGACTGGACAGGACGAT-3'；
SMS071 reference informations:Positioned at 50212646 of No. 11 chromosome；Allele 1 is C, and allele 2 is T；It is located at
In the exon 2 of corresponding gene.
SNP is numbered:SMS072；Gene Name:sheep_OMYHC2X；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:143:5'- GATGCACATGGCAAACACCC -3', downstream primer SEQ ID NO:144: 5'-
TTCTGCGTCACCGTCAACCC-3'；
SMS072 reference informations:Positioned at 29023084 of No. 11 chromosome；Allele 1 is G, and allele 2 is A；It is located at
In 37th exon of corresponding gene.
SNP is numbered:SMS073；Gene Name:sheep_UBB；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:145:5'- TTCTTCGCTCCTCCCGACGCTCAC -3', downstream primer SEQ ID NO:146: 5'-
TTTCGGCGGCGCAGGGACACTA-3'；
SMS073 reference informations:Positioned at 32807859 of No. 11 chromosome；Allele 1 is G, and allele 2 is C；It is located at
In 3rd exon of corresponding gene.
SNP is numbered:SMS074；Gene Name:sheep_ADCK3；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:147:5'- CATCAGGGCTGCTGCTAACC -3', downstream primer SEQ ID NO:148: 5'-
CCTTCCCACAAACACCAAAA-3'；
SMS074 reference informations:Positioned at 28124588 of No. 12 chromosome；Allele 1 is T, and allele 2 is C；It is located at
In 15th exon of corresponding gene.
SNP is numbered:SMS075；Gene Name:sheep_MYBPH；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:149:5'- TTCCCGCCCAGCATACGACT-3', downstream primer SEQ ID NO:150: 5'-
GCCAGGCCCAGAATCAAGCA-3'；
SMS075 reference informations:Positioned at 344555 of No. 12 chromosome；Allele 1 is G, and allele 2 is A；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS076；Gene Name:sheep_KIF1B；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:151:5'- GATTCTCCAATGAGGCAAAC -3', downstream primer SEQ ID NO:152: 5'-
CAGCAGCAGTAATGCTCACAGA-3'；
SMS076 reference informations:Positioned at 41402275 of No. 12 chromosome；Allele 1 is G, and allele 2 is T；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS077；Gene Name:sheep_VIM；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:153:5'- CACATACTTCGTGCAGCAAA -3', downstream primer SEQ ID NO:154: 5'-
CAGAAAGGCACTTGAAAGCT-3'；
SMS077 reference informations:Positioned at 30755105 of No. 13 chromosome；Allele 1 is G, and allele 2 is A；It is located at
In 9th exon of corresponding gene.
SNP is numbered:SMS078；Gene Name:sheep_GNAS；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:155:5'- CCCAACTGAAGGGCACAAAG -3', downstream primer SEQ ID NO:156: 5'-
ACCACCACCACCACCACCAC-3'；
SMS078 reference informations:Positioned at 56777673 of No. 13 chromosome；Allele 1 is G, and allele 2 is A；It is located at
In the exon 2 of corresponding gene.
SNP is numbered:SMS079；Gene Name:sheep_COX4I1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:157:5'- GAATGTTGGCGACCAGAGTA -3', downstream primer SEQ ID NO:158: 5'-
CTGTATGTATCTCCCTTCAAAT-3'；
SMS079 reference informations:Positioned at 11250117 of No. 14 chromosome；Allele 1 is G, and allele 2 is A；It is located at
In the exon 2 of corresponding gene.
SNP is numbered:SMS080；Gene Name:sheep_PHKB；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:159:5'- ACAGATTGTCGTGGAGGTTT -3', downstream primer SEQ ID NO:160: 5'-
GACTCAAGGGTCAAGGATGG-3'；
SMS080 reference informations:Positioned at 15620047 of No. 14 chromosome；Allele 1 is C, and allele 2 is T；It is located at
In 29th exon of corresponding gene.
SNP is numbered:SMS081；Gene Name:sheep_MYBPC2；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:161:5'- AGGACATTGCGGACCTGACG-3', downstream primer SEQ ID NO:162: 5'-
GGAACTGAATCCAAGCCACC-3'；
SMS081 reference informations:Positioned at 55636182 of No. 14 chromosome；Allele 1 is T, and allele 2 is G；It is located at
In 13rd exon of corresponding gene.
SNP is numbered:SMS082；Gene Name:sheep_TNNT1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:163:5'- CCCGTCAATCCCGCCAACTC -3', downstream primer SEQ ID NO:164: 5'-
CGCCACCAGCTCCTCTTCCT-3'；
SMS082 reference informations:Positioned at 59550799 of No. 14 chromosome；Allele 1 is G, and allele 2 is C；It is located at
In 4th exon of corresponding gene.
SNP is numbered:SMS083；Gene Name:sheep_GOT2；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:165:5'- TTTATTTTCTCCCACTACAG -3', downstream primer SEQ ID NO:166: 5'-
TTTTCCACCAGAGTTTACAT-3'；
SMS083 reference informations:Positioned at 25868954 of No. 14 chromosome；Allele 1 is C, and allele 2 is G；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS084；Gene Name:sheep_CKM；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:167:5'- AGGAGCGGAGGGCAAGATGA -3', downstream primer SEQ ID NO:168: 5'-
CTGCTGCTGGCTTCGGGAAT-3'；
SMS084 reference informations:Positioned at 52026336 of No. 14 chromosome；Allele 1 is A, and allele 2 is G；It is located at
In 4th exon of corresponding gene.
SNP is numbered:SMS085；Gene Name:sheep_EIF4G2；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:169:5'- TGGGTAATTTTGTATGTTGC -3', downstream primer SEQ ID NO:170: 5'-
TTTTCCTCCTCCTTTCAGTT-3'；
SMS085 reference informations:Positioned at 41435339 of No. 15 chromosome；Allele 1 is T, and allele 2 is C；It is located at
In 5th exon of corresponding gene.
SNP is numbered:SMS086；Gene Name:sheep_RPS3；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:171:5'- TTGCTTTTGGTTAGTTTGTC -3', downstream primer SEQ ID NO:172: 5'-
CTGTTGTTCTGCTACGTTTA-3'；
SMS086 reference informations:Positioned at 52781908 of No. 15 chromosome；Allele 1 is T, and allele 2 is C；It is located at
In the exon 2 of corresponding gene.
SNP is numbered:SMS087；Gene Name:sheep_SLN；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:173:5'- GTCAGGGCATACAGAAGATAG -3', downstream primer SEQ ID NO:174: 5'-
TAGGAGGTAAGGACAAGCAG-3'；
SMS087 reference informations:Positioned at 16723859 of No. 15 chromosome；Allele 1 is G, and allele 2 is C；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS088；Gene Name:sheep_CRYAB；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:175:5'- GTCACAAGGTTTTCATTCACT -3', downstream primer SEQ ID NO:176: 5'-
TTACTTCATCCCTGTCGTCT-3'；
SMS088 reference informations:Positioned at 21674987 of No. 15 chromosome；Allele 1 is C, and allele 2 is T；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS089；Gene Name:sheep_CCDC63；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:177:5'- TGGGAACTAGCAAAGGGTCT -3', downstream primer SEQ ID NO:178: 5'-
ATGTGGCTGCCTCATTTATC-3'；
SMS089 reference informations:Positioned at 54295463 of No. 17 chromosome；Allele 1 is G, and allele 2 is A；It is located at
In 11st exon of corresponding gene.
SNP is numbered:SMS090；Gene Name:sheep_RPLP0；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:179:5'- ATTCTCCCTTACCTTATTGGC -3', downstream primer SEQ ID NO:180: 5'-
TTGGTGTCTTTATCCTCCCT-3'；
SMS090 reference informations:Positioned at 62189330 of No. 17 chromosome；Allele 1 is G, and allele 2 is A；It is located at
In 5th exon of corresponding gene.
SNP is numbered:SMS091；Gene Name:sheep_SYNM；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:181:5'- GGGAACAGGAGGTTAGCAAG -3', downstream primer SEQ ID NO:182: 5'-
ACAATAGTAGTCAGCTTTAGG-3'；
SMS091 reference informations:Positioned at 6723895 of No. 18 chromosome；Allele 1 is A, and allele 2 is G；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS092；Gene Name:sheep_HSPCA；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:183:5'- AGCCAACATGAAACTAGGAA -3', downstream primer SEQ ID NO:184: 5'-
GCATAAGGGGAGGATAAGAT -3'；
SMS092 reference informations:Positioned at 65639925 of No. 18 chromosome；Allele 1 is A, and allele 2 is G；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS093；Gene Name:sheep_RPL32；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:185:5'- ATCAGAATCACCAATCCCAATG -3', downstream primer SEQ ID NO:186: 5'-
AGCAGAACAGGCAGAAGACC -3'；
SMS093 reference informations:Positioned at 56205952 of No. 19 chromosome；Allele 1 is A, and allele 2 is G；It is located at
In 4th exon of corresponding gene.
SNP is numbered:SMS094；Gene Name:sheep_KLHL31；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:187:5'- TGGCTGTGATGGATGGATTT -3', downstream primer SEQ ID NO:188: 5'-
ATGCTTGGCTTGATTTCTTG -3'；
SMS094 reference informations:Positioned at 6966976 of No. 20 chromosome；Allele 1 is T, and allele 2 is C；Positioned at pair
It answers in the exon 2 of gene.
SNP is numbered:SMS095；Gene Name:sheep_RPL10A；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:189:5'- TACGAGGCGGTGCGGGAAGT -3', downstream primer SEQ ID NO:190: 5'-
TGGAGCCACAAGGGAGGAGA -3'；
SMS095 reference informations:Positioned at 9537284 of No. 20 chromosome；Allele 1 is G, and allele 2 is A；Positioned at pair
It answers in the exon 2 of gene.
SNP is numbered:SMS096；Gene Name:sheep_MALAT1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:191:5'- GGACGCAAACATACAAGATA -3', downstream primer SEQ ID NO:192: 5'-
TTCCGCACCACCACAAATAA -3'；
SMS096 reference informations:Positioned at 42986088 of No. 21 chromosome；Allele 1 is A, and allele 2 is G；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS097；Gene Name:sheep_ACTN3；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:193:5'- CCCGACCTCATTGACTACGC -3', downstream primer SEQ ID NO:194: 5'-
ACCTTCTGCATCCAGCATCTT -3'；
SMS097 reference informations:Positioned at 43894323 of No. 21 chromosome；Allele 1 is C, and allele 2 is T；It is located at
In 7th exon of corresponding gene.
SNP is numbered:SMS098；Gene Name:sheep_TRPM5；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:195:5'- ATCGGTGCCTTTGAGCTTGGAC -3', downstream primer SEQ ID NO:196: 5'-
AGCGGGCTGGACACTCACCTTC -3'；
SMS098 reference informations:Positioned at 48608255 of No. 21 chromosome；Allele 1 is G, and allele 2 is A；It is located at
In 3rd exon of corresponding gene.
SNP is numbered:SMS099；Gene Name:sheep_LDHA；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:197:5'- AACCCAGTGGCTCCCATTTA -3', downstream primer SEQ ID NO:198: 5'-
CAGTTGCCCTTCAAACCAGA -3'；
SMS099 reference informations:Positioned at 25633875 of No. 21 chromosome；Allele 1 is C, and allele 2 is T；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS100；Gene Name:sheep_AHNAK；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:199:5'- TTGAAGTGGACATCAGGCATCT -3', downstream primer SEQ ID NO:200: 5'-
AGGGCCAAACGTAAAGGGTG -3'；
SMS100 reference informations:Positioned at 40266946 of No. 21 chromosome；Allele 1 is G, and allele 2 is A；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS101；Gene Name:sheep_DTNA；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:201:5'- AGGGGAAATAGTTCAAAGTC -3', downstream primer SEQ ID NO:202: 5'-
TCATTCTTACTGGATGGAGG -3'；
SMS101 reference informations:Positioned at 22668109 of No. 23 chromosome；Allele 1 is G, and allele 2 is A；It is located at
In 22nd exon of corresponding gene.
SNP is numbered:SMS102；Gene Name:sheep_MYOM1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:203:5'- TCCACCAGGGAAGCCCATTT -3', downstream primer SEQ ID NO:204: 5'-
GGAAGGCGTAACAGCCAGCA -3'；
SMS102 reference informations:Positioned at 37599526 of No. 23 chromosome；Allele 1 is T, and allele 2 is C；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS103；Gene Name:sheep_MYOM1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:205:5'- GGAGACCACCCAGCTAGGCA -3', downstream primer SEQ ID NO:206: 5'-
CCGACGGTCAGAGGAAGTGC -3'；
SMS103 reference informations:Positioned at 37693255 of No. 23 chromosome；Allele 1 is G, and allele 2 is T；It is located at
In 34th exon of corresponding gene.
SNP is numbered:SMS104；Gene Name:sheep_UQCRC2；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:207:5'- CATAGAGGAGCATCTGGAAT -3', downstream primer SEQ ID NO:208: 5'-
AACTTACTTAGCAGCTTGGA -3'；
SMS104 reference informations:Positioned at 19706140 of No. 24 chromosome；Allele 1 is A, and allele 2 is G；It is located at
In 12nd exon of corresponding gene.
SNP is numbered:SMS105；Gene Name:sheep_MYLPF；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:209:5'- TCTGCCCAACCCTCACCCTT -3', downstream primer SEQ ID NO:210: 5'-
GCCTTTGGTTAGCCTTCAGT -3'；
SMS105 reference informations:Positioned at 26577894 of No. 24 chromosome；Allele 1 is A, and allele 2 is G；It is located at
In 3rd exon of corresponding gene.
SNP is numbered:SMS106；Gene Name:sheep_MDH2；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:211:5'- TTCCCTACAAGACGGATTTACT -3', downstream primer SEQ ID NO:212: 5'-
ACCGGATTTGAGATGATGCA -3'；
SMS106 reference informations:Positioned at 34170741 of No. 24 chromosome；Allele 1 is C, and allele 2 is T；It is located at
In 4th exon of corresponding gene.
SNP is numbered:SMS107；Gene Name:sheep_SRL；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:213:5'- GCTACGACGATGCCCAGTTC -3', downstream primer SEQ ID NO:214: 5'-
TGCCCTCCCACCTTGCTCCT -3'；
SMS107 reference informations:Positioned at 3421119 of No. 24 chromosome；Allele 1 is G, and allele 2 is A；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS108；Gene Name:sheep_ATP2A1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:215:5'- GCAGCTCAGCTCAATCAGTCG -3', downstream primer SEQ ID NO:216: 5'-
CCCATAGTGACGCATCTTCG -3'；
SMS108 reference informations:Positioned at 25933153 of No. 24 chromosome；Allele 1 is T, and allele 2 is C；It is located at
In the exon 2 of corresponding gene.
SNP is numbered:SMS109；Gene Name:sheep_ALDOA；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:217:5'- AGCACTGGCTGGTTGGAAGA -3', downstream primer SEQ ID NO:218: 5'-
ATTGGTGCCTGGTGTTGTCT -3'；
SMS109 reference informations:Positioned at 26221544 of No. 24 chromosome；Allele 1 is T, and allele 2 is C；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS110；Gene Name:sheep_COX6A2；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:219:5'- TCTGGAGGCATCAGCCAAGC -3', downstream primer SEQ ID NO:220: 5'-
TCCGCACCAAGGTACACCGA -3'；
SMS110 reference informations:Positioned at 27474866 of No. 24 chromosome；Allele 1 is A, and allele 2 is G；It is located at
In the exon 2 of corresponding gene.
SNP is numbered:SMS111；Gene Name:sheep_YWHAG；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:221:5'- GGCCTAAACCATAAACAGAC -3', downstream primer SEQ ID NO:222: 5'-
TTTGGGTACTTCCTAGCTCATC -3'；
SMS111 reference informations:Positioned at 34280448 of No. 24 chromosome；Allele 1 is T, and allele 2 is G；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS112；Gene Name:sheep_ACTN2；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:223:5'- CTCTGCTCTGGTCCACTTCA -3', downstream primer SEQ ID NO:224: 5'-
GCCTTTGCTGGCTATGTAAT -3'；
SMS112 reference informations:Positioned at 9392640 of No. 25 chromosome；Allele 1 is A, and allele 2 is G；Positioned at pair
It answers in the 3rd exon of gene.
SNP is numbered:SMS113；Gene Name:sheep_EIF4EBP2；Aligning primer left and right ends information is:Draw upstream
Object SEQ ID NO:225:5'- GACTGTCCGTAACCATCTGC -3', downstream primer SEQ ID NO:226: 5'-
CCAGTAACCCACCATCTAAT -3'；
SMS113 reference informations:Positioned at 26443157 of No. 25 chromosome；Allele 1 is C, and allele 2 is T；It is located at
In 3rd exon of corresponding gene.
SNP is numbered:SMS114；Gene Name:sheep_VDAC2；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:227:5'- TAAGTCTGTTTTGTACCTGCTG -3', downstream primer SEQ ID NO:228: 5'-
TTGGCACTGTCAAAGGTCAT -3'；
SMS114 reference informations:Positioned at 30852607 of No. 25 chromosome；Allele 1 is A, and allele 2 is G；It is located at
In 7th exon of corresponding gene.
SNP is numbered:SMS115；Gene Name:sheep_LDB3；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:229:5'- TTACTCCCTCGTGCCGTCTG -3', downstream primer SEQ ID NO:230: 5'-
GGGCAGGTTTGGTTCATTTC -3'；
SMS115 reference informations:Positioned at 40948899 of No. 25 chromosome；Allele 1 is C, and allele 2 is T；It is located at
In 4th exon of corresponding gene.
SNP is numbered:SMS116；Gene Name:sheep_ACTA1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:231:5'- TCCTTCCACAGGGCTTCGTT -3', downstream primer SEQ ID NO:232: 5'-
CTGGCTTCGCTGTCCACCTT -3'；
SMS116 reference informations:Positioned at 773489 of No. 25 chromosome；Allele 1 is T, and allele 2 is G；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS117；Gene Name:sheep_MYOZ1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:233:5'- CTGTATCCAACACCCAGTCCA -3', downstream primer SEQ ID NO:234: 5'-
GGCTCTGACCAGCAACACTATC -3'；
SMS117 reference informations:Positioned at 29497559 of No. 25 chromosome；Allele 1 is A, and allele 2 is G；It is located at
In 3rd exon of corresponding gene.
SNP is numbered:SMS118；Gene Name:sheep_VDAC3；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:235:5'- TCACTTTCGTGCCACATTTC -3', downstream primer SEQ ID NO:236: 5'-
AGAGTCGGTCACAGCAAACA -3'；
SMS118 reference informations:Positioned at 35738105 of No. 26 chromosome；Allele 1 is C, and allele 2 is T；It is located at
In 8th exon of corresponding gene.
SNP is numbered:SMS119；Gene Name:sheep_ASB5；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:237:5'- TTTAGTAAGGGTTCACATAG -3', downstream primer SEQ ID NO:238: 5'-
AAGATTACACCTGATCCCAC -3'；
SMS119 reference informations:Positioned at 6462798 of No. 26 chromosome；Allele 1 is T, and allele 2 is A；Positioned at pair
It answers in the 1st exon of gene.
SNP is numbered:SMS120；Gene Name:sheep_ACSL1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:239:5'- ACGACGACTGCTCTTTAGGG -3', downstream primer SEQ ID NO:240: 5'-
CTCATTTCCTGGGATTAGAT -3'；
SMS120 reference informations:Positioned at 13920513 of No. 26 chromosome；Allele 1 is C, and allele 2 is T；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS121；Gene Name:sheep_PDLIM3；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:241:5'- GTGCCACTTTGTTAGGTATT -3', downstream primer SEQ ID NO:242: 5'-
TTCAACTGTTTGGGAGGGAG -3'；
SMS121 reference informations:Positioned at 14449882 of No. 26 chromosome；Allele 1 is C, and allele 2 is T；It is located at
In 1st exon of corresponding gene.
SNP is numbered:SMS122；Gene Name:sheep_PDHA1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:243:5'- GATGCTGCTCAGTTTGCTAC -3', downstream primer SEQ ID NO:244: 5'-
TCTCAATCTACACGCCTTCT -3'；
SMS122 reference informations:Positioned at 16836850 of X chromosomes；Allele 1 is C, and allele 2 is T；Positioned at pair
It answers in the 11st exon of gene.
SNP is numbered:SMS123；Gene Name:sheep_PGK1；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:245:5'- GCTAGTAGCCAAGGGATGTA -3', downstream primer SEQ ID NO:246: 5'-
GACAAAGCGAATCAGAATAA -3'；
SMS123 reference informations:Positioned at 65732105 of X chromosomes；Allele 1 is C, and allele 2 is T；Positioned at pair
It answers in the 9th exon of gene.
SNP is numbered:SMS124；Gene Name:sheep_SMPX；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:247:5'- ACTCCTTGACCCACATCCAC -3', downstream primer SEQ ID NO:248: 5'-
TGTTATTGCCGTTTCTTACTTC -3'；
SMS124 reference informations:Positioned at 19018105 of X chromosomes；Allele 1 is C, and allele 2 is T；Positioned at pair
It answers in the exon 2 of gene.
SNP is numbered:SMS125；Gene Name:sheep_PHKA1；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:249:5'- CATTTGGGCATAAGGTCAGC -3', downstream primer SEQ ID NO:250: 5'-
GATCTTGGAGTAATAAGACAG -3'；
SMS125 reference informations:Positioned at 61945475 of X chromosomes；Allele 1 is T, and allele 2 is C；Positioned at pair
It answers in the 23rd exon of gene.
SNP is numbered:SMS126；Gene Name:sheep_RPL10；Aligning primer left and right ends information is:Sense primer
SEQ ID NO:251:5'- AGGCTAATGATTGGGCACTT -3', downstream primer SEQ ID NO:252: 5'-
GATTCGCATCTTCGACTTGG -3'；
SMS126 reference informations:Positioned at 77230913 of X chromosomes；Allele 1 is C, and allele 2 is A；Positioned at pair
It answers in the 4th exon of gene.
SNP is numbered:SMS127；Gene Name:sheep_FHL1；Aligning primer left and right ends information is:Sense primer SEQ
ID NO:253:5'- CACAGAACAGGGTGAGCAGC -3', downstream primer SEQ ID NO:254: 5'-
AGATAAAGCCCCACTGAGAT -3'；
SMS127 reference informations:Positioned at 93880114 of X chromosomes；Allele 1 is A, and allele 2 is T；Positioned at pair
It answers in the 1st exon of gene.
Sequence table
<110>Hubei Duo Gao Biology Breedings Science and Technology Ltd.
<120>The liquid-phase chip of sheep meat SNP marker and its composition
<160> 254
<170> SIPOSequenceListing 1.0
<210> 1
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 1
gccatctgta tttcctttat tc 22
<210> 2
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 2
cactgttatc aaacccttct g 21
<210> 3
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 3
tttatgactt ttggttggat 20
<210> 4
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 4
agaatttgta ggcatgagat 20
<210> 5
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 5
atttattagg cccctgagct 20
<210> 6
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 6
tcttaacacc catatttcga 20
<210> 7
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 7
accaggagac caggtcaaat 20
<210> 8
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 8
tggtgaaggt ccataagtcc 20
<210> 9
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 9
acttcagggt ttccttctcc 20
<210> 10
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 10
gtaatcattt ccaagggtct 20
<210> 11
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 11
ttggctctgt cggcagtttc 20
<210> 12
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 12
tctgggtcgg cacgtctcgt 20
<210> 13
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 13
agctgctata cagtgaagaa 20
<210> 14
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 14
gtaactttgc ctgtgattga 20
<210> 15
<211> 17
<212> DNA
<213>Sheep (Ovis aries)
<400> 15
acaacagcct cattccc 17
<210> 16
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 16
ctcattattg actacttagc g 21
<210> 17
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 17
aagcctcacc gtcaatgttc 20
<210> 18
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 18
ccaagtcaag tgggaagcag 20
<210> 19
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 19
tccaggatgc catttctacc c 21
<210> 20
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 20
gccagcgaga tgatgtgata 20
<210> 21
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 21
tgggacaagg gatagaggaa 20
<210> 22
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 22
ttgccaatga ggtctgaaat ag 22
<210> 23
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 23
tgggttgcca tcccttctcc 20
<210> 24
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 24
ttcagtgggt ggtgtcagta 20
<210> 25
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 25
tcacaaagag gctgagcaga 20
<210> 26
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 26
acactcatca gactttccat 20
<210> 27
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 27
gtgtatctca ggcatcaagg 20
<210> 28
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 28
tctaaagagg gtttcccaaa 20
<210> 29
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 29
ttttaggatg tttgggtgtt 20
<210> 30
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 30
tgtttggcac gaaggaacta 20
<210> 31
<211> 23
<212> DNA
<213>Sheep (Ovis aries)
<400> 31
gaacaatgac ctgctctgga tgg 23
<210> 32
<211> 23
<212> DNA
<213>Sheep (Ovis aries)
<400> 32
gctcaccttg atgtcaggga act 23
<210> 33
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 33
tagaggctca ccgattcatg 20
<210> 34
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 34
caaatagtcc gacaaacacc 20
<210> 35
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 35
gcatcttcgt ggcatgggac a 21
<210> 36
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 36
cagttggttt gctgggtggg 20
<210> 37
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 37
gcagccaaag ccaacaccaa 20
<210> 38
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 38
cagccacatc taaagacagg 20
<210> 39
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 39
tccagtacgt tggtccataa 20
<210> 40
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 40
tcatctcatc ccagccttca 20
<210> 41
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 41
agattgcctc ccaacctaca 20
<210> 42
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 42
caagatatta caagaaccct 20
<210> 43
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 43
tgtctcagaa gagggcattg 20
<210> 44
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 44
ctcccggagt tacttggtat 20
<210> 45
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 45
catagtagcc agtccagcag 20
<210> 46
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 46
catgagcatt gcatcagtaa 20
<210> 47
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 47
agccaacatg aaactaggaa 20
<210> 48
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 48
gcataagggg aggataagat 20
<210> 49
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 49
gatcagcctc cgttctttag c 21
<210> 50
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 50
acgactcctt caccaccttt 20
<210> 51
<211> 24
<212> DNA
<213>Sheep (Ovis aries)
<400> 51
tttccctcta ttgctattac ttcc 24
<210> 52
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 52
ctgtgaccta cctgccctcc 20
<210> 53
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 53
catcatcttt gggaacacta 20
<210> 54
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 54
aacctgccct aaaactaact 20
<210> 55
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 55
aatgtcagtg ccagtttcca 20
<210> 56
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 56
tacttagcca cggcatcttt 20
<210> 57
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 57
tgcttcatac ctactcttcc 20
<210> 58
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 58
atcttcatgc ctaacaactc 20
<210> 59
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 59
ttgagttcca gcatccttgt 20
<210> 60
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 60
aatcttaccc tctttcttag tg 22
<210> 61
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 61
cagaactgat cgaaaagacc 20
<210> 62
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 62
tcaaaggagt gacaacaaga g 21
<210> 63
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 63
agaccacgac cttacgaaca 20
<210> 64
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 64
ataaacctag tggctgctga 20
<210> 65
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 65
aaaccttacg accctcctgg 20
<210> 66
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 66
cccttgcttt gcgaacctct 20
<210> 67
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 67
tcttgtggtt tggtcagcat 20
<210> 68
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 68
cgttcaactg taacaaggct 20
<210> 69
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 69
ttcacaagcc aggggtcggg 20
<210> 70
<211> 24
<212> DNA
<213>Sheep (Ovis aries)
<400> 70
agtggaatgt ctatttcttc tcag 24
<210> 71
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 71
gcccagacgg ctttgttgag 20
<210> 72
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 72
tgacgtggtg gtgaaggatt 20
<210> 73
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 73
tgctcaccaa gcctccctcc 20
<210> 74
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 74
caccatgacc ggcaagaaaa 20
<210> 75
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 75
atcattcttg gcactgtttc 20
<210> 76
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 76
gatttccttc ctatcaaccc 20
<210> 77
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 77
atcagctctg tccttgcctt aa 22
<210> 78
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 78
gtctcatccc tgaccctttc t 21
<210> 79
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 79
tccacctcag atcatcagtt a 21
<210> 80
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 80
gcacctttac cctcaccttg 20
<210> 81
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 81
tctcagccaa gaagaagcca cc 22
<210> 82
<211> 24
<212> DNA
<213>Sheep (Ovis aries)
<400> 82
cgacatcatc aatgccaaac aata 24
<210> 83
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 83
tcttcccttt cagaccttat 20
<210> 84
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 84
ttcaattccg tccaaaccta 20
<210> 85
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 85
cctttgtctt caagttcatc 20
<210> 86
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 86
tctgccatta gttggtctgc 20
<210> 87
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 87
ccacggaaag aagccatact 20
<210> 88
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 88
taatgcctga aagctgtaat 20
<210> 89
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 89
agtctggacc ttctttcttc 20
<210> 90
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 90
ttttccaagt tctactctgt 20
<210> 91
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 91
tccacctcag atcatcagtt a 21
<210> 92
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 92
gcacctttac cctcaccttg 20
<210> 93
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 93
cctctgcttt ccctgcttct 20
<210> 94
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 94
taacccatcc ctcttctccc 20
<210> 95
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 95
tccacctcag atcatcagtt a 21
<210> 96
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 96
gcacctttac cctcaccttg 20
<210> 97
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 97
nnncagtcct tggctgttcg 20
<210> 98
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 98
tctcaagagt taccacgaat 20
<210> 99
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 99
tggttatgaa ataggcagta 20
<210> 100
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 100
attataccag cagtccagag 20
<210> 101
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 101
agtcccgcca aaggcaatca 20
<210> 102
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 102
gtaccagcag taaggcagag 20
<210> 103
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 103
gctttgccag tgagtagaat 20
<210> 104
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 104
tcatggcctt taccaatcct 20
<210> 105
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 105
ttgataggtt cctccttgat 20
<210> 106
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 106
caggcttctt gccagatgtt 20
<210> 107
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 107
caggaatgcc tattggacac 20
<210> 108
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 108
ttcgcacaat ctgtagtcac gt 22
<210> 109
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 109
atcacagctc agccctcctc 20
<210> 110
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 110
cttccgtctt cacttcatcc 20
<210> 111
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 111
ttgcagacac catcccagag 20
<210> 112
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 112
cccgtcaaag cctgagccac 20
<210> 113
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 113
ttttcggtgc taccacaggc 20
<210> 114
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 114
atctccaccc tcctcactcc 20
<210> 115
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 115
tccatgaaag caggagtgta 20
<210> 116
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 116
gacagcagaa gccaagaaaa 20
<210> 117
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 117
agtcttaaca ggagcacttg cc 22
<210> 118
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 118
cctcagcctc cctcaccttg 20
<210> 119
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 119
ttgtggtttt attcagcgtt 20
<210> 120
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 120
ttctttgctt cacttctccc 20
<210> 121
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 121
tcttaggtgc cacatacatt 20
<210> 122
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 122
tcgatgaggc agaaccaacc 20
<210> 123
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 123
tccacctcag atcatcagtt a 21
<210> 124
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 124
gcacctttac cctcaccttg 20
<210> 125
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 125
aagctggaag atggccctaa 20
<210> 126
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 126
tgatgacacc cacagcgact 20
<210> 127
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 127
gtagattagt attgctggtt 20
<210> 128
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 128
tttatcccac tactgttcat 20
<210> 129
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 129
tacatgggtt ctcaaatagc 20
<210> 130
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 130
agaaagggag gaagaggaaa 20
<210> 131
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 131
aagtgggcaa tctgaaaggg 20
<210> 132
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 132
atcgagcgga tgatgaaaca 20
<210> 133
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 133
gggttaggaa ggagcagtca 20
<210> 134
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 134
ccttatttgc taccatttcg 20
<210> 135
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 135
agaatgagtc ccttcagtta 20
<210> 136
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 136
cgtgagggtg ggattaacaa 20
<210> 137
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 137
tttgatgcct tctacgatat 20
<210> 138
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 138
ttctgtttcc cttcctgcta 20
<210> 139
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 139
taaactgctc ccagaaccaa 20
<210> 140
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 140
ttagagggaa acccgtgaaa 20
<210> 141
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 141
ttggtggagc tattcaaagg g 21
<210> 142
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 142
cgaaggactg gacaggacga t 21
<210> 143
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 143
gatgcacatg gcaaacaccc 20
<210> 144
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 144
ttctgcgtca ccgtcaaccc 20
<210> 145
<211> 24
<212> DNA
<213>Sheep (Ovis aries)
<400> 145
ttcttcgctc ctcccgacgc tcac 24
<210> 146
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 146
tttcggcggc gcagggacac ta 22
<210> 147
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 147
catcagggct gctgctaacc 20
<210> 148
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 148
ccttcccaca aacaccaaaa 20
<210> 149
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 149
ttcccgccca gcatacgact 20
<210> 150
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 150
gccaggccca gaatcaagca 20
<210> 151
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 151
gattctccaa tgaggcaaac 20
<210> 152
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 152
cagcagcagt aatgctcaca ga 22
<210> 153
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 153
cacatacttc gtgcagcaaa 20
<210> 154
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 154
cagaaaggca cttgaaagct 20
<210> 155
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 155
cccaactgaa gggcacaaag 20
<210> 156
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 156
accaccacca ccaccaccac 20
<210> 157
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 157
gaatgttggc gaccagagta 20
<210> 158
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 158
ctgtatgtat ctcccttcaa at 22
<210> 159
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 159
acagattgtc gtggaggttt 20
<210> 160
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 160
gactcaaggg tcaaggatgg 20
<210> 161
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 161
aggacattgc ggacctgacg 20
<210> 162
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 162
ggaactgaat ccaagccacc 20
<210> 163
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 163
cccgtcaatc ccgccaactc 20
<210> 164
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 164
cgccaccagc tcctcttcct 20
<210> 165
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 165
tttattttct cccactacag 20
<210> 166
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 166
ttttccacca gagtttacat 20
<210> 167
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 167
aggagcggag ggcaagatga 20
<210> 168
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 168
ctgctgctgg cttcgggaat 20
<210> 169
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 169
tgggtaattt tgtatgttgc 20
<210> 170
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 170
ttttcctcct cctttcagtt 20
<210> 171
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 171
ttgcttttgg ttagtttgtc 20
<210> 172
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 172
ctgttgttct gctacgttta 20
<210> 173
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 173
gtcagggcat acagaagata g 21
<210> 174
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 174
taggaggtaa ggacaagcag 20
<210> 175
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 175
gtcacaaggt tttcattcac t 21
<210> 176
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 176
ttacttcatc cctgtcgtct 20
<210> 177
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 177
tgggaactag caaagggtct 20
<210> 178
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 178
atgtggctgc ctcatttatc 20
<210> 179
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 179
attctccctt accttattgg c 21
<210> 180
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 180
ttggtgtctt tatcctccct 20
<210> 181
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 181
gggaacagga ggttagcaag 20
<210> 182
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 182
acaatagtag tcagctttag g 21
<210> 183
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 183
agccaacatg aaactaggaa 20
<210> 184
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 184
gcataagggg aggataagat 20
<210> 185
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 185
atcagaatca ccaatcccaa tg 22
<210> 186
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 186
agcagaacag gcagaagacc 20
<210> 187
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 187
tggctgtgat ggatggattt 20
<210> 188
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 188
atgcttggct tgatttcttg 20
<210> 189
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 189
tacgaggcgg tgcgggaagt 20
<210> 190
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 190
tggagccaca agggaggaga 20
<210> 191
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 191
ggacgcaaac atacaagata 20
<210> 192
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 192
ttccgcacca ccacaaataa 20
<210> 193
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 193
cccgacctca ttgactacgc 20
<210> 194
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 194
accttctgca tccagcatct t 21
<210> 195
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 195
atcggtgcct ttgagcttgg ac 22
<210> 196
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 196
agcgggctgg acactcacct tc 22
<210> 197
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 197
aacccagtgg ctcccattta 20
<210> 198
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 198
cagttgccct tcaaaccaga 20
<210> 199
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 199
ttgaagtgga catcaggcat ct 22
<210> 200
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 200
agggccaaac gtaaagggtg 20
<210> 201
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 201
aggggaaata gttcaaagtc 20
<210> 202
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 202
tcattcttac tggatggagg 20
<210> 203
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 203
tccaccaggg aagcccattt 20
<210> 204
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 204
ggaaggcgta acagccagca 20
<210> 205
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 205
ggagaccacc cagctaggca 20
<210> 206
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 206
ccgacggtca gaggaagtgc 20
<210> 207
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 207
catagaggag catctggaat 20
<210> 208
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 208
aacttactta gcagcttgga 20
<210> 209
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 209
tctgcccaac cctcaccctt 20
<210> 210
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 210
gcctttggtt agccttcagt 20
<210> 211
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 211
ttccctacaa gacggattta ct 22
<210> 212
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 212
accggatttg agatgatgca 20
<210> 213
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 213
gctacgacga tgcccagttc 20
<210> 214
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 214
tgccctccca ccttgctcct 20
<210> 215
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 215
gcagctcagc tcaatcagtc g 21
<210> 216
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 216
cccatagtga cgcatcttcg 20
<210> 217
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 217
agcactggct ggttggaaga 20
<210> 218
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 218
attggtgcct ggtgttgtct 20
<210> 219
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 219
tctggaggca tcagccaagc 20
<210> 220
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 220
tccgcaccaa ggtacaccga 20
<210> 221
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 221
ggcctaaacc ataaacagac 20
<210> 222
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 222
tttgggtact tcctagctca tc 22
<210> 223
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 223
ctctgctctg gtccacttca 20
<210> 224
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 224
gcctttgctg gctatgtaat 20
<210> 225
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 225
gactgtccgt aaccatctgc 20
<210> 226
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 226
ccagtaaccc accatctaat 20
<210> 227
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 227
taagtctgtt ttgtacctgc tg 22
<210> 228
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 228
ttggcactgt caaaggtcat 20
<210> 229
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 229
ttactccctc gtgccgtctg 20
<210> 230
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 230
gggcaggttt ggttcatttc 20
<210> 231
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 231
tccttccaca gggcttcgtt 20
<210> 232
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 232
ctggcttcgc tgtccacctt 20
<210> 233
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 233
ctgtatccaa cacccagtcc a 21
<210> 234
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 234
ggctctgacc agcaacacta tc 22
<210> 235
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 235
tcactttcgt gccacatttc 20
<210> 236
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 236
agagtcggtc acagcaaaca 20
<210> 237
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 237
tttagtaagg gttcacatag 20
<210> 238
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 238
aagattacac ctgatcccac 20
<210> 239
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 239
acgacgactg ctctttaggg 20
<210> 240
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 240
ctcatttcct gggattagat 20
<210> 241
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 241
gtgccacttt gttaggtatt 20
<210> 242
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 242
ttcaactgtt tgggagggag 20
<210> 243
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 243
gatgctgctc agtttgctac 20
<210> 244
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 244
tctcaatcta cacgccttct 20
<210> 245
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 245
gctagtagcc aagggatgta 20
<210> 246
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 246
gacaaagcga atcagaataa 20
<210> 247
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 247
actccttgac ccacatccac 20
<210> 248
<211> 22
<212> DNA
<213>Sheep (Ovis aries)
<400> 248
tgttattgcc gtttcttact tc 22
<210> 249
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 249
catttgggca taaggtcagc 20
<210> 250
<211> 21
<212> DNA
<213>Sheep (Ovis aries)
<400> 250
gatcttggag taataagaca g 21
<210> 251
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 251
aggctaatga ttgggcactt 20
<210> 252
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 252
gattcgcatc ttcgacttgg 20
<210> 253
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 253
cacagaacag ggtgagcagc 20
<210> 254
<211> 20
<212> DNA
<213>Sheep (Ovis aries)
<400> 254
agataaagcc ccactgagat 20
Claims (5)
1. the liquid-phase chip of sheep meat SNP marker and its composition, it is characterised in that contain following 127 SNP marker sites
Information, SNP site primer sequence and Gene Name corresponding to them are given by the 5th of the case study on implementation in patent specification
Go out, 127 SNP site numbers are SMS001~SMS127.
2. sheep meat SNP marker as described in claim 1, feature SMS001~SMS127 includes SNP site
Sequence information combines.
3. sheep meat SNP marker as described in claim 1, feature SMS001~SMS127 includes SNP site
The complementary series or homologous sequence of sequence.
4. the liquid-phase chip of sheep meat as described in claim 1, the carrier described in feature is liquid phase carrier.
5. the liquid-phase chip of sheep meat as described in claim 1, feature contains the SNP upstream and downstream primers,
SMS001 sense primers are：SEQ ID NO:1, SMS001 downstream primer is：SEQ ID NO:2, SMS002 sense primers are：
SEQ ID NO:3 ..., SMS127 sense primers are：SEQ ID NO:253, SMS127 downstream primers are：SEQ ID NO:254,
Assistance application for related molecular marker.
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| CN201810633358.6A CN108707677A (en) | 2018-06-20 | 2018-06-20 | The liquid-phase chip of sheep meat SNP marker and its composition |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810633358.6A CN108707677A (en) | 2018-06-20 | 2018-06-20 | The liquid-phase chip of sheep meat SNP marker and its composition |
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| CN108707677A true CN108707677A (en) | 2018-10-26 |
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| CN201810633358.6A Pending CN108707677A (en) | 2018-06-20 | 2018-06-20 | The liquid-phase chip of sheep meat SNP marker and its composition |
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| CN109943649A (en) * | 2019-05-08 | 2019-06-28 | 刘学峰 | Sheep intramuscular fat content molecular labeling and its naked eyed test cultivation in apply |
| CN110079611A (en) * | 2019-05-08 | 2019-08-02 | 刘学峰 | It sheep intramuscular fat and inosine acid content molecular labeling and its is applied in breed of variety |
| CN110687236A (en) * | 2019-10-30 | 2020-01-14 | 陕西师范大学 | Method for evaluating freezing and thawing degree of meat based on iTRAQ marker protein |
| CN111979338A (en) * | 2020-09-02 | 2020-11-24 | 刘学峰 | Molecular biological method for breeding high-quality mutton sheep |
| CN112695107A (en) * | 2021-03-23 | 2021-04-23 | 中国农业大学 | Growth performance SNP locus combination of meat sheep and application thereof |
| CN113249495A (en) * | 2021-06-29 | 2021-08-13 | 西北农林科技大学 | Sheep liquid phase chip and application thereof |
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| CN114854869A (en) * | 2022-04-15 | 2022-08-05 | 中国农业科学院北京畜牧兽医研究所 | SNP (Single nucleotide polymorphism) marker related to goat heat-resistant character and application |
| CN116516025A (en) * | 2023-06-09 | 2023-08-01 | 吉林省农业科学院 | SNP mutation site of beef cattle HADHB gene and application thereof |
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| CN109943649B (en) * | 2019-05-08 | 2019-12-03 | 黑龙江省农业科学院畜牧兽医分院 | Sheep intramuscular fat content molecular labeling and its naked eyed test cultivation in apply |
| CN110079611B (en) * | 2019-05-08 | 2020-01-07 | 黑龙江省农业科学院畜牧兽医分院 | Molecular marker for contents of intramuscular fat and inosinic acid of sheep and application of molecular marker in variety cultivation |
| CN109943649A (en) * | 2019-05-08 | 2019-06-28 | 刘学峰 | Sheep intramuscular fat content molecular labeling and its naked eyed test cultivation in apply |
| CN110687236B (en) * | 2019-10-30 | 2022-05-17 | 陕西师范大学 | Method for evaluating freezing and thawing degree of meat based on iTRAQ marker protein |
| CN110687236A (en) * | 2019-10-30 | 2020-01-14 | 陕西师范大学 | Method for evaluating freezing and thawing degree of meat based on iTRAQ marker protein |
| CN111979338A (en) * | 2020-09-02 | 2020-11-24 | 刘学峰 | Molecular biological method for breeding high-quality mutton sheep |
| CN112695107A (en) * | 2021-03-23 | 2021-04-23 | 中国农业大学 | Growth performance SNP locus combination of meat sheep and application thereof |
| CN112695107B (en) * | 2021-03-23 | 2021-06-08 | 中国农业大学 | Growth performance SNP locus combination of meat sheep and application thereof |
| CN115029451B (en) * | 2021-06-29 | 2023-09-19 | 西北农林科技大学 | Sheep liquid phase chip and application thereof |
| CN113249495A (en) * | 2021-06-29 | 2021-08-13 | 西北农林科技大学 | Sheep liquid phase chip and application thereof |
| CN115029451A (en) * | 2021-06-29 | 2022-09-09 | 西北农林科技大学 | Sheep liquid phase chip and application thereof |
| CN114231642A (en) * | 2022-01-07 | 2022-03-25 | 新疆畜牧科学院畜牧研究所 | Molecular marker related to Erdos fine wool sheep wool fiber diameter character, specific primer pair and application |
| CN114231642B (en) * | 2022-01-07 | 2024-01-02 | 新疆畜牧科学院畜牧研究所 | Molecular marker and specific primer pair related to diameter character of wool fibers of Erdos fine wool sheep and application |
| CN114854869B (en) * | 2022-04-15 | 2022-12-09 | 中国农业科学院北京畜牧兽医研究所 | SNP (Single nucleotide polymorphism) marker related to goat heat-resistant character and application |
| CN114854869A (en) * | 2022-04-15 | 2022-08-05 | 中国农业科学院北京畜牧兽医研究所 | SNP (Single nucleotide polymorphism) marker related to goat heat-resistant character and application |
| CN116516025A (en) * | 2023-06-09 | 2023-08-01 | 吉林省农业科学院 | SNP mutation site of beef cattle HADHB gene and application thereof |
| CN116516025B (en) * | 2023-06-09 | 2023-11-17 | 吉林省农业科学院(中国农业科技东北创新中心) | SNP mutation site of beef cattle HADHB gene and application thereof |
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| CN117385061B (en) * | 2023-12-13 | 2024-03-15 | 中国农业科学院北京畜牧兽医研究所 | Molecular marker related to Hu sheep growth traits and application thereof |
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Application publication date: 20181026 |