CN108704032A - Chinese medicine composition and its preparation method and application for conditioning and treating hepatopathy - Google Patents

Abstract

The present invention relates to a kind of Chinese medicine composition and its preparation method and application for conditioning and treating hepatopathy, Chinese medicine composition of the present invention is prepared by the raw material of following weight：15~25 parts of ganoderma spove powder, 15~25 parts of Ganodenna Lucidum P.E, 15~25 parts of extract of Zhuling, 15~25 parts of dandelion extract, 15~25 parts of Schisandra chinens P.E.The Chinese medicine composition chooses the Chinese medicine of integration of drinking and medicinal herbs, according to the theory of Chinese medicine " monarch ", scientific compatibility is carried out to above-mentioned five kinds of Pharmaceutical ingredients, propose effective pharmaceutical composition, the immunity of hepatopath can be enhanced, the discharge for promoting vivotoxin does not have any toxic side effect to promoting hepatopathy rehabilitation significant effect to human body.

Description

Chinese medicine composition and its preparation method and application for conditioning and treating hepatopathy

Technical field

The present invention relates to technical field of Chinese medicine, more particularly to a kind of Chinese medicine composition for conditioning and treating hepatopathy and Preparation method and application.

Background technology

Hepatopathy is common disease and frequently-occurring disease in China, and hepatopathy can be usually divided into virus hepatitis, alcoholic liver disease, drug Several major class such as property hepatic injury, fatty liver, hepatic sclerosis and liver cancer.Hepatitis can be divided into hepatitis A virus (HAV), hepatitis B again Malicious (HBV), Hepatitis C Virus (HCV), Hepatitis D virus (HDV), Hepatitis E virus, fulminant hepatitis and non-icteric type Hepatitis etc., wherein the harm of hepatitis B is maximum, incidence is also higher.As the improvement of people's living standards, alcoholic liver and fat Fat liver etc. " rich people's disease " incidence improves year by year, and number of patients even alreadys exceed hepatitis B patient.Fatty liver is one kind by various A kind of pathological state that reason causes liver fat deposition excessive.About 10% simple fatty liver can be to steatohepatitis rank Section, wherein there is 20%-40% that can develop into liver fibrosis and hepatic sclerosis, eventually leads to liver cancer.Do not come to be turned by fatty liver people The number of liver fibrosis and hepatic sclerosis is turned to about in 400-800 ten thousand.Alcoholic liver disease situation also very severe, can not be ignored.It is long Phase heavy drinking makes liver damage, causes various alcoholic liver disease, can be divided into mild alcoholic liver, alcoholic liver, wine by degree of injury Essence fatty liver, alcoholic fibrosis and alcoholic cirrhosis.Conservative estimation China alcoholic liver patient numbers should be 50,000,000 Left and right, is such as not treated in time, and 60% alcohol hepatopath finally translates into hepatic sclerosis even liver cancer.

By hepatitis, fatty liver, alcoholic liver patient numbers calculate liver fibrosis crowd should be 10,000,000 or so.China has more than 7000000 liver cirrhosis patient, and number of patients is on the increase.Cause the cause of disease of hepatic sclerosis very much, virus hepatitis can be divided into Hepatic sclerosis, alcoholic cirrhosis, metabolic hepatic sclerosis, autoimmune cirrhosis etc..In China, most of hepatic sclerosis are hepatitis Hepatic sclerosis afterwards, small part are alcoholic cirrhosis and Cirrhosis In Schistosomiasis.

This great disease of common harmfulness in face of hepatopathy, although modern medicine can effectively control the symptom of hepatopathy, But toxic side effect is big, treatment cost is expensive.Moreover, it is a more difficult thing that hepatopathy, which is got well, can utilize a variety of The composition of Chinese medicine carries out hepatopathy rehabilitation conditioning, and it is a new issue for being difficult to accelerate hepatopathy rehabilitation.At present, it has been reported that have The drug for adjusting liver function has had tens of kinds, such as cordyceps sinensis, rheum officinale, Radix Notoginseng, Radix Astragali, kuh-seng, the fruit of Chinese wolfberry, Schisandra chinensis, ganoderma lucidum, strand Stock indigo plant etc., still, the Chinese medicine preparation reported at present is very complicated, some include even ten several Chinese medicines, " are as the saying goes medicines Three points of poison ", formula is excessively complicated, is easy to introduce the ingredient to human body toxic side effect.

Invention content

Based on this, it is necessary to a kind of Chinese medicine composition for conditioning and treating hepatopathy is provided, is had no toxic and side effect to human body, it can To enhance the immunity of hepatopath, promote hepatopathy rehabilitation.

A kind of Chinese medicine composition for conditioning and treating hepatopathy, the Chinese medicine composition by following weight raw material Composition：

Ganoderma lucidum and ganoderma spove powder, for the monarch drug in a prescription of the present invention.Ganoderma lucidum (Ganoderma lucidum), as drug First recorded in《Sheng Nong's herbal classic》, reinforcement can be moistened and strengthened, strengthen the body resistance to consolidate the constitution, promote longevity, there is high medical value and health care work( Effect.Ganoderma lucidum belongs to a kind of medicinal fungi plant of Basidiomycetes Polyporaceae Ganoderma, and Reishi sporule is ganoderma lucidum in growth and maturity The reproduction cell ejected from cap, also referred to as basidiospore have and inhibit tumour, improve immunity, is hypoglycemic and anti-oxidant Equal multi-efficiencies.Effect of ganoderma lucidum mystery is in addition to containing abundant ganoderma lucidum polysaccharide, terpene, adenosine, amino acid and micro member in it A variety of pharmacological components such as element, especially organic germanium, it can change acidic constitution of human body, remove phthiremia impurity.

Umbellate pore furgus is the ministerial drug of the present invention, contains ergosterol, α hydroxyls -24 carbonic acid, biotin, water in extract of Zhuling The cancer preventing and treatings effective ingredients such as dissolubility poly sugar compounds such as grifola polysaccharide and crude protein.Experiment shows that grifola polysaccharide can improve T Cellular immune function has adjustment effect to humoral immunity, and the weakling of immunologic hypofunction can be made hale and hearty.Study table Bright, contained grifola polysaccharide in umbellate pore furgus also increases the accumulation of hepatic glycogen, promotes the effect of liver cell regeneration.Umbellate pore furgus can improve pair The phagocytic activity of cell promotes the immune of cell, and acting with promotion hepatitis surface antibody.

Dandelion, for the adjutant of the present invention, dandelion enters Liver Channel.Liver protection, clear liver poison, and Chinese medicine thinks, dandelion bitter but sweet flavor Cold in nature, Return liver, stomach have clearing heat and detoxicating, carbuncle that disappears dissipating bind, anti-inflammatory, cool blood, diuresis, cholagogic, hypocatharsis, stomach invigorating, anti-cancer etc. a variety of Function.Choline in dandelion extract can accelerate phosphatide to synthesize, thus can accelerate cell repair speed, have to liver cell bright Aobvious protective effect.

Schisandra chinensis makes medicine, schizandrin A, B prime, C prime, alcohol first, alcohol second, ester in Chinese Magnoliavine Fruit alcohol extract for the present invention First, ester the second grade has obvious protective function to animal liver cell damage caused by chemical toxicant, can inhibit the release of transaminase, makes third Propylhomoserin transferase (ALT) activity reduces.

Above-mentioned Chinese medicine composition chooses the Chinese medicine of integration of drinking and medicinal herbs, according to the theory of Chinese medicine " monarch ", to above-mentioned five Kind Pharmaceutical ingredients carry out scientific compatibility, it is proposed that effective pharmaceutical composition can enhance the immunity of hepatopath, promote internal The discharge of toxin does not have any toxic side effect to promoting hepatopathy rehabilitation significant effect to human body.

In one of the embodiments, the ganoderma spove powder be 18~22 parts, the Ganodenna Lucidum P.E be 18~ 22 parts, the extract of Zhuling is 18~22 parts, and the dandelion extract is 18~22 parts, and the Schisandra chinens P.E is 18 ~22 parts.

Further, the ganoderma spove powder is 20 parts, and the Ganodenna Lucidum P.E is 20 parts, the extract of Zhuling It it is 20 parts, the dandelion extract is 20 parts, and the Schisandra chinens P.E is 20 parts.

The sporoderm-broken rate of the ganoderma spove powder is more than 95% in one of the embodiments,.

The ganoderma spove powder is Reishi sporule through the Ultramicro-powder obtained by broken wall, institute in one of the embodiments, The broken wall step for stating ganoderma spove powder includes：After carrying out Microwave Pretreatment to Reishi sporule under vacuum, liquid is carried out Nitrogen deep cooling freezing processing freezes to the temperature of the Reishi sporule and is less than or equal to -30 DEG C, then carries out air-flow crushing.

Reishi sporule have double-layer cell wall, be mainly chitin, cause its tough and tensile cell wall, acid and alkali-resistance, it is resistance to enzymolysis, The properties such as anti-digestion, pressure-resistant heat resistanceheat resistant, hinder human body digesting and assimilating and utilizing to effective component of ganoderma lucidum spore powder.It first passes through micro- Wave pretreatment makes the chitin fiber of Reishi sporule wall be broken, and improves the brittleness of Reishi sporule wall, and reduce in lucidum spore powder The content of water, then liquid nitrogen deep freezing processing is carried out, the brittleness of Reishi sporule wall is further increased, is carried out to lucidum spore powder When air-flow crushing, water content is lower, and the brittle degree of conidial cell wall is higher, easier broken wall, and shell-broken effect is better.

The Ganodenna Lucidum P.E, extract of Zhuling, dandelion extract and Schisandra chinensis extraction in one of the embodiments, Object be respectively ganoderma lucidum, umbellate pore furgus, dandelion and Schisandra chinensis after crushed, with volumetric concentration be 65%~75% ethanol water soak 40h~50h is carried, is filtered, filtrate is obtained, concentrates the medicinal extract of gained after filtrate, the relative density of the medicinal extract is 1.1~1.15.

Another object of the present invention is to provide a kind of preparation method of the Chinese medicine composition for conditioning and treating hepatopathy, including Following steps：

Reishi sporule is taken, after carrying out Microwave Pretreatment to Reishi sporule under vacuum, is carried out at liquid nitrogen deep freezing Reason freezes to the temperature of the Reishi sporule and is less than or equal to -30 DEG C, then carries out air-flow crushing, obtain ganoderma spove powder；

After respectively crushing ganoderma lucidum, umbellate pore furgus, dandelion and Schisandra chinensis, it is 65%~75% ethyl alcohol to use volumetric concentration respectively Aqueous solution extracts 40h~50h, and filtering obtains filtrate, obtains the medicinal extract that relative density is 1.1~1.15 after concentrating filtrate, that is, distinguishes Obtain Ganodenna Lucidum P.E, extract of Zhuling, dandelion extract and Schisandra chinens P.E；

Take 15~25 parts of ganoderma spove powders, 15~25 parts of Ganodenna Lucidum P.Es, 15~25 parts of umbellate pore furgus Extract, 15~25 parts of dandelion extracts and 15~25 parts of Schisandra chinens P.Es, each component is mixed, you can.

The temperature of the microwave treatment is 30 DEG C~40 DEG C in one of the embodiments, and the time is 10min~20min.

In this way, to Reishi sporule progress microwave treatment it is possible to prevente effectively from the oxygen of Reishi sporule under vacuum low-temperature environment Change, reduces influence of the oxygen in air to nutriment in Reishi sporule, and low temperature environment effectively can overcome microwave to spirit The heat damage that the heat effect of Ganoderma lucidum spore generates can fully maintain the ganoderma lucidum polysaccharide contained in Reishi sporule powder raw material, three The effect of active ingredients high-content such as terpene, organic germanium, raising product.

In one of the embodiments, the Ganodenna Lucidum P.E be ganoderma lucidum fruitbody after crushed, be with volumetric concentration 65%~75% ethanol water extraction 40h~50h, filtering, filtrate concentration after gained medicinal extract, the medicinal extract it is relatively close Degree is 1.1~1.15.

Further, the mass volume ratio of the ganoderma lucidum fruitbody and the ethanol water is 1g：(15~20) mL.

The mass volume ratio of the umbellate pore furgus and the ethanol water is 1g in one of the embodiments,：(15~20) mL。

The mass volume ratio of the dandelion and the ethanol water is 1g in one of the embodiments,：(10~ 20)mL。

The mass volume ratio of the Schisandra chinensis and the ethanol water is 1g in one of the embodiments,：(10~ 20)mL。

In one of the embodiments, the preparation method further include above-mentioned Chinese medicine composition is dried, powder processed Step.

Further, the step of the drying is that the Chinese medicine composition is carried out microwave drying under vacuum environment.Such as This, play the role of uniformly, rapid draing while can also sterilize.Water is the strongly absorbing medium of microwave, and microwave can allow microorganism In hydrone formed galvanic couple polarity and with electric field change and high speed transport, cause membrane structure to rupture, hydrogen between cellular elements Key destroys, to have the function that sterilization.

Specifically, vacuum microwave drying case can be used, processing is dried.

Another object of the present invention is to provide a kind of above-mentioned Chinese medicine composition and is preparing for assisting in hepatic drug Application.

Another object of the present invention is to provide a kind of pharmaceutical preparation, is with the above-mentioned Chinese medicine group for conditioning and treating hepatopathy Conjunction object is active constituent.

Further, the pharmaceutical preparation can be with the Chinese medicine composition and medically acceptable pharmaceutic adjuvant, The various dosage forms for being suitable for clinical application, including but not limited to capsule, granule, piece are made by the conventional method of pharmaceutical preparation The dosage forms such as agent, oral solution.

The invention has the advantages that：

1), the present invention chooses the Chinese medicine of five tastes integration of drinking and medicinal herbs, it then follows the principles of formulating prescriptions of Chinese medicine " monarch ", with ganoderma lucidum With ganoderma spove powder be monarch drug in a prescription, umbellate pore furgus is ministerial drug, dandelion is adjutant, Schisandra chinensis is to make medicine, reasonable formula, all medicine phases The effect of closing, playing tonifying liver tonifying speen and tonifying kidney, promoting blood circulation toxin expelling, can enhance the immunity of hepatopath, aobvious to hepatopathy rehabilitation effect It writes, has the characteristics that have no toxic and side effect to human body, is stable and controllable for quality, having a good application prospect in hepatopathy rehabilitation field.

2), Chinese medicine composition of the present invention selects ganoderma spove powder, Ganodenna Lucidum P.E, extract of Zhuling, dandelion to carry It is that raw material carries out prescription to take object and Schisandra chinens P.E, more convenient to use to which taking dose is small.

Specific implementation mode

To facilitate the understanding of the present invention, below will to invention is more fully described, and give the present invention compared with Good embodiment.But the present invention can realize in many different forms, however it is not limited to embodiment described herein.Phase Instead, purpose of providing these embodiments is makes the disclosure of the present invention more thorough and comprehensive.

Unless otherwise defined, all of technologies and scientific terms used here by the article and belong to the technical field of the present invention The normally understood meaning of technical staff is identical.Used term is intended merely to description tool in the description of the invention herein The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term as used herein "and/or" includes one or more phases Any and all combinations of the Listed Items of pass.

A kind of Chinese medicine composition for conditioning and treating hepatopathy of embodiment 1

1, the formula of Chinese medicine composition：20 parts of ganoderma spove powder, 20 parts of Ganodenna Lucidum P.E, 20 parts of extract of Zhuling, 20 parts of dandelion extract, 20 parts of Schisandra chinens P.E.

2, each component raw material

1), ganoderma spove powder

Ganoderma spove powder is the drying spore of On Polyporaceae red sesame or purple sesame through the Ultramicro-powder obtained by broken wall, is pressed NY/T1677-2008《The measurement of ganoderma spove powder sporoderm-broken rate》, it is desirable that broken rate must not be less than 95%, can also pass through Commercially available purchase can also be prepared by the following method and obtain：

Reishi sporule is taken, pretreatment 15min is carried out to Reishi sporule using microwave under 30 DEG C~40 DEG C of cryogenic conditions, Liquid nitrogen deep freezing processing is carried out to Reishi sporule using liquid nitrogen deep instant freezer, is frozen to donor center temperature to -35 DEG C, then Supersonic airstream broken wall is carried out at -10 DEG C using supersonic jet mill, crushing mesh number is 800 mesh, obtains wall-broken ganoderma spore Powder, sporoderm-broken rate reach 99.5%.

2), Ganodenna Lucidum P.E

Ganodenna Lucidum P.E is extract made of ganoderma lucidum fruitbody warp adds, can be by commercially available purchase, also can be by following Method is prepared：

Ganoderma lucidum fruitbody is taken, coarse powder (30 mesh) is ground into, is 1g by the mass volume ratio of ganoderma lucidum coarse powder and ethyl alcohol：18mL, With the alcohol solvent of 70vol%, after impregnating ganoderma lucidum coarse powder 48h, filtrate is collected in filtering, and ethyl alcohol is recovered under reduced pressure and is concentrated into opposite Density is the medicinal extract of 1.1~1.15 (65 DEG C), obtains Ganodenna Lucidum P.E, extract yield 8.6%.

3), extract of Zhuling

Extract of Zhuling be On Polyporaceae umbellate pore furgus sclerotium through plus made of extract, can by commercially available purchase, Also it can be prepared by the following method and obtain：

Dry sclerotia is taken, coarse powder (30 mesh) is ground into, is 1g by the mass volume ratio of umbellate pore furgus coarse powder and ethyl alcohol： 15mL, with the alcohol solvent of 70vol%, after impregnating umbellate pore furgus coarse powder 48h, filtering collects filtrate, ethyl alcohol is recovered under reduced pressure and is concentrated into Relative density is the medicinal extract of 1.1~1.15 (65 DEG C), obtains extract of Zhuling, extract yield 9.1%.

4), dandelion extract

Dandelion extract be dandelion herb through plus made of extract, can by commercially available purchase, also can by with Lower section method is prepared：

Dandelion herb is ground into coarse powder (30 mesh), is 1g by the mass volume ratio of dandelion coarse powder and ethyl alcohol：15mL, After the alcohol dipping dandelion coarse powder 48h of 70vol%, filtrate is collected in filtering, and ethyl alcohol is recovered under reduced pressure and is concentrated into relative density For the medicinal extract of 1.1~1.15 (65 DEG C), dandelion extract, extract yield 7.5% are obtained.

5), Schisandra chinens P.E

Schisandra chinens P.E is the dry mature fruit warp of magnoliaceae schisandra plus manufactured extract, can be passed through Commercially available purchase can also be prepared by the following method and obtain：

The dry mature fruit of Schisandra chinensis is ground into coarse powder (30 mesh), by the mass volume ratio of Schisandra chinensis coarse powder and ethyl alcohol For 1g：10mL, after the alcohol dipping Schisandra chinensis coarse powder 48h of 70vol%, filtering collects filtrate, ethyl alcohol is recovered under reduced pressure and concentrates To the medicinal extract that relative density is 1.1~1.15 (65 DEG C), Schisandra chinens P.E, extract yield 8.8% are obtained.

Embodiment 2 is used for the granule of auxiliary treatment hepatopathy

2000 grams of ganoderma spove powder in above-described embodiment 1,2000 grams of Ganodenna Lucidum P.E, umbellate pore furgus extraction are weighed respectively 2000 grams of object, 2000 grams of dandelion extract, 2000 grams of Schisandra chinens P.E.

The mixture of above-mentioned four kinds of extracts and ganoderma spove powder is placed in vacuum microwave drying case, it is dry in 40 DEG C Dry 30min, then powder processed, is processed into particle electuary, per 3 grams of parcel.

Embodiment 3

Embodiment 3 is substantially the same manner as Example 2, the difference is that：Ganoderma spove powder is 250 grams, ganoderma lucidum is extracted Object is 150 grams, extract of Zhuling is 150 grams, and dandelion extract is 230 grams, and Schisandra chinens P.E is 220 grams.

Embodiment 4

Embodiment 4 is substantially the same manner as Example 2, the difference is that：Ganoderma spove powder is 200 grams, ganoderma lucidum is extracted Object is 250 grams, extract of Zhuling is 250 grams, and dandelion extract is 150 grams, and Schisandra chinens P.E is 150 grams.

Embodiment 5

Embodiment 5 is substantially the same manner as Example 2, the difference is that：Ganoderma spove powder is 180 grams, ganoderma lucidum is extracted Object is 220 grams, extract of Zhuling is 220 grams, and dandelion extract is 180 grams, and Schisandra chinens P.E is 200 grams.

Comparative example 1

Comparative example 1 is substantially the same manner as Example 2, the difference is that：Ganoderma spove powder is 100 grams, ganoderma lucidum is extracted Object is 200 grams, extract of Zhuling is 200 grams, and dandelion extract is 200 grams, and Schisandra chinens P.E is 200 grams.

Comparative example 2

Comparative example 2 is substantially the same manner as Example 2, the difference is that：Ganoderma spove powder is 200 grams, ganoderma lucidum is extracted Object is 200 grams, extract of Zhuling is 100 grams, and dandelion extract is 200 grams, and Schisandra chinens P.E is 200 grams.

Comparative example 3

Comparative example 3 is substantially the same manner as Example 2, the difference is that：Ganoderma spove powder is 200 grams, ganoderma lucidum is extracted Object is 200 grams, extract of Zhuling is 200 grams, and dandelion extract is 100 grams, and Schisandra chinens P.E is 200 grams.

Comparative example 4

Comparative example 4 is substantially the same manner as Example 2, the difference is that：Ganoderma spove powder is 200 grams, ganoderma lucidum is extracted Object is 200 grams, extract of Zhuling is 200 grams, and dandelion extract is 200 grams, and Schisandra chinens P.E is 100 grams.

The Chinese medicine composition of 6 Application Example 2~5 of embodiment and comparative example 1~4 causes D-Gal (D-Gla) The Protection of acute liver

Experiment material：80 Kunming mouses, male, weight 20g ± 2g are randomly divided into 8 groups, every group 10：Model group, The granule group of Normal group, bifendate positive controls (25mg), embodiment 2~5 and comparative example 1~4 (100mg).Ad lib, drinking-water, natural light irradiation before experiment.

Experimental method：Each medication group mouse distinguishes gastric infusion 1 time daily, and normal group and model group are with isometric physiology salt Water gavage 20mL/ (kgd), continuous 10 days.After last medication about 6h, in addition to Normal group, remaining equal abdomen of each group mouse Chamber injects 1%D- amine-galactoses normal saline solution (200mg/kg).It is deprived of food but not water, takes a blood sample and take afterwards for 24 hours hepatic tissue, examine It surveys serum transaminase ALT (alanine aminotransferase), AST (glutamic-oxalacetic transaminease) level and liver MDA (malonaldehyde) is horizontal, as a result see The following table 1.

Table 1

Group	ALT(U/L)	AST(U/L)	MDA(nmol/mgprot)
				Normal group	28.25±5.52	113.16±9.24	10.52±1.948
Model group	163.45±11.87	275.61±18.5	34.36±6.45
				Bifendate control group	81.25±8.97	201.35±10.28	19.49±4.36
2 granule group of embodiment	106.75±9.65	213.19±11.64	21.57±2.80
				3 granule group of embodiment	138.94±9.87	234.28±12.13	27.07±3.58
4 granule group of embodiment	136.75±9.65	237.19±11.64	27.50±2.81
				5 granule group of embodiment	123.75±9.65	209.15±10.73	24.57±2.55
1 granule group of comparative example	155.02±10.73	265.43±12.4	30.89±3.52
				2 granule group of comparative example	154.37±9.52	263.36±11.72	31.97±3.24
3 granule group of comparative example	155.94±9.87	266.28±12.32	32.07±3.58
				4 granule group of comparative example	157.64±9.54	268.46±10.25	31.79±3.65

As a result：

Serum transaminase is the sensitive indicator of hepatocellular damage, and the most commonly used is ALT and AST.MDA is that free radical is attacked The peroxidatic reaction of lipid product of biomembrane initiation is hit, the content in tissue can reflect level of lipid peroxidation, reflect indirectly Go out the degree that cell is attacked and damaged by free radical.

Compared with Normal group, ALT, AST are horizontal in model group mice serum and liver MDA level significantly increases, Show that D-Gal destroys the antioxidant system of liver, damage is caused to liver cell.And bifendate group, embodiment 2~5 with And 1~4 granule group of comparative example is compared with model group, can reduce to varying degrees ALT and AST enzymes activity and The level of MDA illustrates to have certain protective action to acute liver damage caused by D-Gal.

But 1~4 above-mentioned five kind of raw material of embodiment 2~5 and comparative example combines the Chinese medicine composition of formation in varing proportions Granule in, it is optimal with the effect of embodiment 2.Model group is compared, although 1~4 granule group of comparative example can be to a certain degree The upper MDA for reducing ALT, AST is horizontal, but effect unobvious；The effect that embodiment 2~5 reduces the MDA levels of ALT, AST is bright It is aobvious, and 2 granule group of embodiment and the significant difference of model control group, D-Gal acute hepatic injury mice blood can be significantly inhibited ALT and AST activity levels in clear, and the reduction of MDA contents is acted on obviously, the denaturation and necrosis of hepatic tissue can be mitigated, it is right Hepatic injury has good protective action.

Embodiment 7 imitates the granule clinical trial with the Chinese medicine composition for promoting hepatopathy rehabilitation effect of embodiment 2 Fruit is verified

Instructions of taking：Sooner or later each daily edible primary, 3 grams every time.

Present inventor began one's study from 2010, and formula has constantly been improved since 8 years, 313 formulas was done and changes Into experiment, there are several hepatopaths to eat the product of the present invention, wherein hepatopath on the books 19, wherein man 11, Female 8,40 years old 3 below, 5 of 40 or more years old, 6 of 50 years old or more, 5 of 60 or more years old, total effective rate reaches 95.3%, obvious effective rate reaches 81.7%, restores normal person after 12 months edible and leads up to 81.6%, now takes passages three patient's cases for example Under：

Huang, man, 43 years old 2010, hepatitis B is diagnosed as through People's Hospital, Hunan Prov., and patient's gastric disorder causing nausea is indigestion and loss of appetite, detests oil, burnout It is weak, eyes are yellow, therapeutic effect is bad for many years, and paddy transaminase continued jitters are checked in 310~385 units per liters to hospital, we It is recommended that Huang on the basis of drug therapy, eats the product of conditioning the embodiment of the present invention 2, adjustment has apparent effect in 6 months Fruit, in being off drug, symptom completely disappears at 1 year, and paddy transaminase still controls very well, present Huang 51 years old, feels Very healthy, now substantially free from taking medicine, check also remains always normal, obtains significant rehabilitation conditioning effect.

46 years old 2012, hepatitis B is diagnosed as through Xiang Ya hospitals of Hunan Province by poplar, man, patient's self-induction malaise, it is indigestion and loss of appetite not It is intended to eat, detests that oil, urine color be yellow, pore membrane xanthochromia, and right flank distending pain occur, paddy transaminase is checked in 310 units per liters to hospital, it is more Year therapeutic effect is bad, it is proposed that Huang on the basis of drug therapy, eats the product of the conditioning present invention, adjusts 8 months It has obvious effects on, in being off drug, symptom completely disappears when 1 year edible, and paddy transaminase still controls very well, present poplar Certain 53 years old, feel very healthy, now substantially free from taking medicine, check also remains always normal, obtains significant rehabilitation Conditioning effect.

41 years old 2012, hepatic sclerosis is diagnosed as through Xiang Ya hospitals of Hunan Province by Liu, female, patient's self-induction general malaise is weak, Indigestion and loss of appetite loss of appetite, urine color Huang, pore membrane xanthochromia, and detest oil, paddy transaminase is checked in 400 units per liters to hospital, and treatment for many years is imitated Fruit is bad, it is proposed that Liu on the basis of drug therapy, improves the product for eating the present invention, conditioning has apparent for 6 months Effect, in being off drug, symptom completely disappears when 1 year edible, and paddy transaminase is down to 50 units per liters, and present Liu 48 In year, feel very healthy, index full recovery always is normal, obtains significant rehabilitation conditioning effect.

Each technical characteristic of embodiment described above can be combined arbitrarily, to keep description succinct, not to above-mentioned reality It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited In contradiction, it is all considered to be the range of this specification record.

Several embodiments of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention Range.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.

Claims (10)

1. a kind of Chinese medicine composition for conditioning and treating hepatopathy, which is characterized in that the Chinese medicine composition is by following parts by weight The raw material of proportioning forms：

2. Chinese medicine composition according to claim 1, which is characterized in that the ganoderma spove powder is 18~22 parts, The Ganodenna Lucidum P.E is 18~22 parts, and the extract of Zhuling is 18~22 parts, and the dandelion extract is 18~22 parts, The Schisandra chinens P.E is 18~22 parts.

3. Chinese medicine composition according to claim 1, which is characterized in that the ganoderma spove powder is 20 parts, described Ganodenna Lucidum P.E is 20 parts, and the extract of Zhuling is 20 parts, and the dandelion extract is 20 parts, the Schisandra chinens P.E It is 20 parts.

4. Chinese medicine composition according to claim 1, which is characterized in that the ganoderma spove powder passes through for Reishi sporule The step of Ultramicro-powder obtained by broken wall, the broken wall includes：Microwave Pretreatment is carried out to the Reishi sporule under vacuum Afterwards, liquid nitrogen deep freezing processing is carried out, freezes to the temperature of the Reishi sporule and is less than or equal to -30 DEG C, then carry out air-flow powder It is broken.

5. Chinese medicine composition according to claim 1, which is characterized in that the Ganodenna Lucidum P.E, extract of Zhuling, Pu Gong English extract and Schisandra chinens P.E be respectively ganoderma lucidum, umbellate pore furgus, dandelion and Schisandra chinensis after crushed, with volumetric concentration be 65% ~75% ethanol water extracts 40h~50h, and filtering obtains filtrate, concentrates the medicinal extract of gained after filtrate, the phase of the medicinal extract It is 1.1~1.15 to density.

6. a kind of preparation method of Chinese medicine composition for conditioning and treating hepatopathy, which is characterized in that include the following steps：

Reishi sporule is taken, after carrying out Microwave Pretreatment to Reishi sporule under vacuum, liquid nitrogen deep freezing processing is carried out, freezes Temperature to the Reishi sporule is less than or equal to -30 DEG C, then carries out air-flow crushing, obtains ganoderma spove powder；

After respectively crushing ganoderma lucidum, umbellate pore furgus, dandelion and Schisandra chinensis, the ethyl alcohol for using volumetric concentration to be 65%~75% respectively is water-soluble Liquid extracts 40h~50h, and filtering obtains filtrate, obtains the medicinal extract that relative density is 1.1~1.15 after concentrating filtrate, that is, respectively obtains Ganodenna Lucidum P.E, extract of Zhuling, dandelion extract and Schisandra chinens P.E；

Take 15~25 parts of ganoderma spove powders, 15~25 parts of Ganodenna Lucidum P.Es, 15~25 parts of umbellate pore furgus extractions Object, 15~25 parts of dandelion extracts and 15~25 parts of Schisandra chinens P.Es, each component is mixed, you can.

7. preparation method according to claim 6, which is characterized in that the temperature of the microwave treatment is 30 DEG C~40 DEG C, Time is 10min~20min.

8. preparation method according to claim 6, which is characterized in that the volumetric concentration of the ethanol water is 70%.

9. application of any Chinese medicine composition of Claims 1 to 5 in preparing the drug for auxiliary treatment hepatopathy.

10. using any Chinese medicine composition of Claims 1 to 5 as the pharmaceutical preparation of active constituent.