CN108703993A - Flourish muscle picks up the preparation process of pain side - Google Patents
Flourish muscle picks up the preparation process of pain side Download PDFInfo
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
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- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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Abstract
The present invention provides a kind of preparation methods of pharmaceutical composition for the treatment of of arthritis (i.e. " flourish muscle picks up pain side "), it includes the following steps：A, the bulk pharmaceutical chemicals of each weight proportion are weighed：10~14 parts of the root of bidentate achyranthes, 4~8 parts of Radix Angelicae Sinensis, 6~12 parts of Radix Angelicae Pubescentis, 4~8 parts of Rhizoma Et Radix Notopterygii, 4~8 parts windproof, 4~8 parts of Radix Glycyrrhizae；B, 8~12 times of a concentration of 50%~90% ethyl alcohol of amount are added, impregnates 20~40min, is heated to 80~85 DEG C, 1~2h of extraction time, extract 1~3 time, filtering, concentrate to get.The preparation process that honor muscle of the invention picks up pain Fang Xin is used as extraction solution using the polarity ethyl alcohol weaker than water, although the extraction scope to polar substances is smaller than water, the small-molecule substances such as main extraction alkaloid, but it is wider than water range to the extraction of apolar substance, and recovery rate is high；And the drug being prepared is used for anti-inflammatory and antalgic, IL-1 β, IL-6, TNF-α content can be reduced, improve IL-10 contents, especially alcohol extract is better than water extract to the adjustment effect of inflammatory factor, and alcohol extract curative effect is better than control group, the use for picking up pain side for clinical flourish muscle provides a kind of new selection.
Description
Technical field
The present invention relates to the preparation processes that flourish muscle picks up pain side.
Background technology
Flourish muscle picks up pain side, is derived from traditional Chinese medical science great master CHEN Ke ji academician《Palace of the Qing Dynasty formula is integrated》Middle treatment heumatism (osteoarthritis)
High frequency, core prescription, including the root of bidentate achyranthes, Radix Angelicae Sinensis, Radix Angelicae Pubescentis, Rhizoma Et Radix Notopterygii, windproof, 6 taste Chinese medicine of Radix Glycyrrhizae.The root of bidentate achyranthes is to tonify the liver and kidney in side
And strong muscles and bones, and the root of bidentate achyranthes can invigorate blood circulation using tonneau podomere tendon and vessel as monarch drug in a prescription.Minister is with Chinese Angelica blood replonishing and blood, with flourish muscles and bones blood vessels.Solely
It is living to be apt to control volt wind, remove long numbness；Notopterygium wind dispelling is wet, sharp joint, analgesic；Windproof expelling wind and eliminating dampness analgesic, three medicine phases are matched, and the numbness of the whole body is removed
Bitterly, to reach wind-damp dispelling, stopping numbness pain as adjutant.Radix Glycyrrhizae, which is arrogated to oneself, controls spasm of limbs, and relieving spasm to stop pain, more coordinating the drug actions of a prescription are to make medicine.All medicines
Filling liver kidney is played altogether, is strengthened the bone, and the benefits of wind-damp dispelling, stopping numbness pain, the etiology and pathogenesis that can be directed to knee osteoarthritis plays therapeutic effect.
Research shows that：Flourish muscle, which picks up pain, can be effectively relieved the clinical symptoms and Ti Zheng [ of Patients with Knee Osteoarthritis;Zhu Dingyu, woods
The wooden south, Wanning wait honor muscle to pick up pain side treatment knee osteoarthritis Lin Chuanliaoxiaoguancha [A]The 24th Chinese combination of Chinese tradiational and Western medicine
Orthopedics and traumatology science nd Annual Meeting collects;C].2017 year .]；With the computer simulation side based on compound-target spot interaction
Method specifies that flourish muscle picks up the active constituent and its action target spot of pain side's treatment osteoarthritis, and the intuitive flourish muscle of display picks up pain side not only
It can inhibit inflammatory Cytokines Expression, relieve pain, it can also be by inhibiting catabolism, stimulation anabolism, inhibiting class flower
Raw acid synthesis etc. has the function that multiple target point protection cartilage [Zheng Chunsong, Fan Zhanbiao, leaf luxuriant sesame, wait the study of computer simulation
Flourish muscle picks up effective substance, action target spot and the Zuo Yongtedian [ of pain side's treatment osteoarthritis;J]Chinese medicine bonesets, and 2017,29
(10)：20-24.]；In addition, also chemically space angle illustrates flourish muscle and picks up pain side and has the function of wide spectrum for computer simulation,
In, filling liver kidney strengthen the bone drug have the function of stimulate articular cartilage in proteoglycan synthesize, and wind-damp dispelling stopping numbness pain drug have
There is anti-inflammatory and antalgic [Zheng Chunsong, leaf luxuriant sesame pay long queue, and waiting, chemically discussion flourish muscle in space picks up pain side's tonifying liver and kidney and strengthening bones and muscles
With the Zuo Yong [ of wind-damp dispelling stopping numbness pain;J]Rheumatism and arthritis, 2018,7 (2)：33-36.].As it can be seen that flourish muscle picks up the treatment of pain side
Knee osteoarthritis has preferable clinical efficacy, and its active constituent has to knee osteoarthritis multiple target point synergistic therapeutic action.
Application number：201710284659.8 denomination of invention：Flourish muscle picks up pain side and its preparation method and application, and the invention is public
It has opened flourish muscle and has picked up pain side and its preparation method and application.Pharmaceutical composition of the present invention, it is by the bulk pharmaceutical chemicals of following weight proportion
The preparation being prepared：10~14 parts of the root of bidentate achyranthes, 4~8 parts of Radix Angelicae Sinensis, 6~12 parts of Radix Angelicae Pubescentis, 4~8 parts of Rhizoma Et Radix Notopterygii, windproof 4~8 parts, Radix Glycyrrhizae
4~8 parts.The disclosure of the invention water extraction process, water itself are polar solvents, mainly extract hydrophilic composition, and extraction scope is wide,
But poor selectivity, easily extracts polysaccharide, and the macromolecular substances such as protein bring difficulty with preparation to differentiating, easily go mouldy, be not easy to store up
It deposits.
Invention content
In order to solve the above-mentioned technical problem, the invention discloses the preparation processes that flourish muscle picks up pain Fang Xin.
The present invention provides a kind of preparation methods of the pharmaceutical composition for the treatment of of arthritis, it includes the following steps：
A, the bulk pharmaceutical chemicals of each weight proportion are weighed：
10~14 parts of the root of bidentate achyranthes, 4~8 parts of Radix Angelicae Sinensis, 6~12 parts of Radix Angelicae Pubescentis, 4~8 parts of Rhizoma Et Radix Notopterygii, 4~8 parts windproof, 4~8 parts of Radix Glycyrrhizae；
B, 8~12 times of a concentration of 50%~90% ethyl alcohol of amount are added, impregnate 20~40min, are heated to 80~85 DEG C, extraction
1~2h of time, extract 1~3 time, filtering, concentrate to get.
It is further preferred that the weight proportion of the bulk pharmaceutical chemicals described in a steps is：
12 parts of the root of bidentate achyranthes, 6 parts of Radix Angelicae Sinensis, 9 parts of Radix Angelicae Pubescentis, 6 parts of Rhizoma Et Radix Notopterygii, 6 parts windproof, 6 parts of Radix Glycyrrhizae.
Wherein, the addition of the ethyl alcohol described in b step is 10 times of amounts.
Wherein, the concentration of alcohol is 70% ethyl alcohol.
Wherein, the dip time is 30min.
Wherein, the heating temperature is 80 DEG C.
Wherein, the extraction time is 2h.
Wherein, the extraction time is 3 times.
Honor muscle of the invention picks up the preparation process of pain Fang Xin, using the polarity ethyl alcohol weaker than water as extraction solution, although right
The extraction scope of polar substances is smaller than water, main to extract the small-molecule substances such as alkaloid, but compares water to the extraction of apolar substance
Range is wider, and recovery rate is high；And the drug being prepared is used for anti-inflammatory analgesic, can reduce IL-1 β, IL-6, TNF-α content, carry
High IL-10 contents, especially alcohol extract are better than water extract to the adjustment effect of inflammatory factor, and alcohol extract curative effect is better than control
Group, the use for picking up pain side for clinical flourish muscle provide a kind of new selection.
Obviously, the above according to the present invention is not being departed from according to the ordinary technical knowledge and customary means of this field
Under the premise of the above-mentioned basic fundamental thought of the present invention, the modification, replacement or change of other diversified forms can also be made.
The specific implementation mode of form by the following examples remakes further specifically the above of the present invention
It is bright.But the range that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to example below.It is all to be based on the above of the present invention
The technology realized all belongs to the scope of the present invention.
Description of the drawings
Husking sterol, ferulic acid, macrotin glycosides, 5-O- methyl visamminols glycosides, glycyrrhizic acid content detection in Fig. 1 compounds
Osthole, Isomperatorin and Notopterol content detection in Fig. 2 compounds
The influence that Fig. 3 concentration of alcohol extracts compound
Fig. 4 solvents measure the influence extracted to compound again
Fig. 5 extraction times extract compound the influence of result response
The influence that Fig. 6 extraction times extract compound
Influence of Fig. 7 differences intervention group to IL-1 β contents in synovial tissue
Influence of Fig. 8 differences intervention group to IL-6 contents in synovial tissue
Influence of Fig. 9 differences intervention group to TNF-α content in synovial tissue
Influence of Figure 10 differences intervention group to IL-10 contents in synovial tissue
Influence of Figure 11 differences intervention group to IL-1 β contents in serum
Influence of Figure 12 differences intervention group to IL-6 contents in serum
Influence of Figure 13 differences intervention group to TNF-α content in serum
Influence of Figure 14 differences intervention group to IL-10 contents in serum
Specific implementation mode
The preparation of 1 drug extract of the present invention of embodiment
It accurately weighs root of bidentate achyranthes 12g, Radix Angelicae Pubescentis 9g, Radix Angelicae Sinensis 6g, Rhizoma Et Radix Notopterygii 6g, windproof 6g, Radix Glycyrrhizae 6g to be placed in round-bottomed flask, extract
Temperature is fixed as 80 DEG C, extracts 10 times of multiple, alcohol solvent ratio 70%, impregnates 30min, after refluxing extraction 1.5h, filters, dense
Contracting is settled to 500mL, takes concentrate, 50% 5 times of dilution in acetonitrile, filter membrane, sample introduction.
The preparation of 2 drug extract of the present invention of embodiment
It accurately weighs root of bidentate achyranthes 12g, Radix Angelicae Pubescentis 9g, Radix Angelicae Sinensis 6g, Rhizoma Et Radix Notopterygii 6g, windproof 6g, Radix Glycyrrhizae 6g to be placed in round-bottomed flask, extract
Temperature is fixed as 80 DEG C, extracts 10 times of multiple, alcohol solvent ratio 70%, impregnates 30min, after refluxing extraction 2h, filters, concentration
It is settled to 500mL, takes concentrate, 50% 5 times of dilution in acetonitrile, filter membrane, sample introduction.
The preparation of 3 drug extract of the present invention of embodiment
It accurately weighs root of bidentate achyranthes 10g, Radix Angelicae Pubescentis 6g, Radix Angelicae Sinensis 4g, Rhizoma Et Radix Notopterygii 4g, windproof 4g, Radix Glycyrrhizae 4g to be placed in round-bottomed flask, extract
Temperature is fixed as 85 DEG C, extracts 8 times of multiple, alcohol solvent ratio 50%, impregnates 20min, after refluxing extraction 1h, filters, concentration
It is settled to 500mL, takes concentrate, 50% 5 times of dilution in acetonitrile, filter membrane, sample introduction.
The preparation of 4 drug extract of the present invention of embodiment
It accurately weighs root of bidentate achyranthes 14g, Radix Angelicae Pubescentis 12g, Radix Angelicae Sinensis 8g, Rhizoma Et Radix Notopterygii 8g, windproof 8g, Radix Glycyrrhizae 8g to be placed in round-bottomed flask, carry
It takes temperature to be fixed as 85 DEG C, extracts 10 times of multiple, alcohol solvent ratio 90% impregnates 40min, after refluxing extraction 2h, filters, dense
Contracting is settled to 500mL, takes concentrate, 50% 5 times of dilution in acetonitrile, filter membrane, sample introduction.
The flourish muscle of embodiment 5 picks up optimize technique prepared by pain side
1 experiment material
1.1 medicinal materials and reference substance source
Reference substance β-ecdysterone (lot number：111638-201706, content 98.4%) it is purchased from Chinese food drug assay
Research institute.The medicinal material root of bidentate achyranthes derives from Xiamen Yan Laifu pharmaceutical Co. Ltds (lot number：171122).
Reference substance Osthole (lot number：111820-201504, content 98.88%), angeloyloxy-columbianetin
(batch：111583-201605, content 98.6%), it is purchased from National Institute for Food and Drugs Control.Medicinal material Radix Angelicae Pubescentis is purchased from Lu
Watchtower medicine company (lot number：1708030352).
Reference substance ferulic acid (lot number：110773-201614, content 99.0%) it is studied purchased from Chinese food drug assay
Institute.Radix Angelicae Sinensis medicinal material is purchased from Fuzhou Ruilai Chuntang Biotechnology Co., Ltd.'s (lot number：195201701).
Reference substance macrotin glycosides (lot number：111522-201712, content 96.2%) 5-O- methyl visamminol glycosides
(lot number：111523-201610, content 96.1%) it is purchased from National Institute for Food and Drugs Control.Medicinal material is windproof to be purchased from good fortune
The state bio tech ltd Rui Laichuntang (lot number：197201702).
Reference substance ammonium glycyrrhetate (batch：110731-201619, content 93.0%), it is purchased from the inspection of Chinese food drug
Determine research institute.Medicinal material Radix Glycyrrhizae is coordinated purchased from Anhui into pharmaceutcal corporation, Ltd's (lot number：17082004).
Reference substance Notopterol (lot number：111820-201504, content 98.88%) it is ground purchased from Chinese food drug assay
Study carefully institute.Medicinal material Rhizoma Et Radix Notopterygii is purchased from Bozhou City Tian Ji pharmaceutcal corporation, Ltds (lot number：170301).
1.2 instrument
Ten a ten thousandth assay balance of CPA225D types (Sai Duolisi scientific instrument Co., Ltd), SHB-III type recirculated waters
Multiplex vavuum pump (Zhengzhou Greatwall Scientific Industrial & Trading Co., Ltd.), LX-07A types multifunctional crusher (the limited public affairs of Shanghai river letter science and technology
Department), HH-Z type digital displays thermostat water bath (Changzhou Guo Hua Electric companies), 1260 efficient liquid phases of Agilent TechnOlOgies
Chromatograph (agilent company), the desk-top ultrasonic cleaner of KQ-500E types (Kunshan Ultrasonic Instruments Co., Ltd.), Milli-Q
Ultra-pure water instrument (MillipOre companies).Chromatographic grade acetonitrile and methanol (German Merck companies), chromatographic grade formic acid (Shanghai Aladdin
Reagent Co., Ltd), remaining reagent is that analysis is pure.
2 methods and result
2.1 chromatographic condition
2.1.1 chromatographic condition
The root of bidentate achyranthes, Radix Angelicae Sinensis, windproof and licorice ingredient, wherein root of bidentate achyranthes detection husking sterol are measured, Radix Angelicae Sinensis detects ferulic acid, windproof
Macrotin glycosides and 5-O- methyl visamminol glycosides are detected, Radix Glycyrrhizae detects glycyrrhizic acid.
Chromatographic column：ThermO BETASIL C18 Analytical chromatographic columns (4.6mm × 250mm, 5 μm), mobile phase：
- 0.1% formic acid (B) of acetonitrile (A), gradient elution (0~5min, 18.0%A → 18.0%A；5~35min, 18.0%A →
30.0%A；35~45min, 30.0%A → 55.0%A；45~54min, 55.0%A → 92.0%A；54~54.5min,
92.0%A → 12.0%A；54.5~60min, 12.0%A → 12.0%A), column temperature：35 DEG C, Detection wavelength：237nm and
317nm；Flow velocity：1.0mL/min, 10 μ L of sample size.Compound chromatogram is shown in Fig. 1.
2.1.2 chromatographic condition
Radix Angelicae Pubescentis and Rhizoma Et Radix Notopterygii ingredient are measured, wherein Radix Angelicae Pubescentis measures Osthole, and Rhizoma Et Radix Notopterygii measures Isomperatorin and Notopterol.
Chromatographic column：ThermO BETASIL C18 Analytical chromatographic columns (4.6mm × 250mm, 5 μm), mobile phase：
Acetonitrile (A)-water (B), gradient elution (0~10min, 49%A → 49%A；10~18min, 49%A → 55%A；18~
25min, 55%A → 70%A；25~42min, 70%A → 85%A；42~45min, 85%A → 90%A；45~45.5min,
90%A → 40%A；45.5~50min, 40%A → 40%A), column temperature：35 DEG C, Detection wavelength：310nm, flow velocity：1.0mL/
Min, 10 μ L of sample size.See Fig. 2
The preparation of 2.2 solution
2.2.1 prepared by reference substance solution
Precision weighs β-ecdysterone, ferulic acid, Notopterol, Isomperatorin, 5-O- methyl visamminols glycosides, Radix Glycyrrhizae
Reference substance 5.15mg, 4.58mg, 5.14mg, 5.48mg, 5.73mg, 4.92mg, 5.00mg, 3.02mg of acid, Osthole,
He is respectively placed in 5mL volumetric flasks, and ferulic acid and glycyrrhizic acid dissolve with 70% methanol and are diluted to scale, other are dissolved with methanol
And be diluted to scale, shake up, respectively obtain mass concentration be 1.03mg/mL β-ecdysterone, 0.916mg/mL ferulic acids,
1.028mg/mL Notopterol, 1.096mg/mL Isomperatorins, 1.146mg/mL5-O- methyl visamminols glycosides, 0.984mg/
The glycyrrhizic acid of mL, the reference substance solution of 1.000mg/mL Ostholes and 604 μ g/mL macrotin glycosides.Above-mentioned reference substance solution is taken,
According to configuring mixed reference substance solution under Chinese Pharmacopoeia one point external standard method assay item：70% methanol dilution is added to obtain β-husking
Sterone 0.103mg/mL, 18.32 μ g/mL of ferulic acid, 60.4 μ g/mL of macrotin glycosides, 57.3 μ g/ of 5-O- methyl visamminols glycosides
The mixed solution of mL, 196.8 μ g/mL of glycyrrhizic acid, for measuring the root of bidentate achyranthes, Radix Angelicae Sinensis, windproof and licorice ingredient, wherein root of bidentate achyranthes detection is sloughed off
Skin sterol, Radix Angelicae Sinensis detect ferulic acid, windproof detection macrotin glycosides and 5-O- methyl visamminol glycosides, and Radix Glycyrrhizae detects glycyrrhizic acid；
70% methanol dilution is added to obtain the mixing of 27.4 μ g/mL of 51.4 μ g/mL of Notopterol, 150 μ g/mL of Osthole and Isomperatorin
Solution, for measuring Radix Angelicae Pubescentis and Rhizoma Et Radix Notopterygii ingredient, wherein Radix Angelicae Pubescentis measures Osthole, and Rhizoma Et Radix Notopterygii measures Isomperatorin and Notopterol.
2.2.2 prepared by test solution
Alcohol extracting：Accurately weighing root of bidentate achyranthes 12g, Radix Angelicae Pubescentis 9g, Radix Angelicae Sinensis 6g, Rhizoma Et Radix Notopterygii 6g, windproof 6g, Radix Glycyrrhizae 6g is placed in round-bottomed flask
In, Extracting temperature is fixed as 80 DEG C, extracts 10 times of multiple, alcohol solvent ratio 70%, impregnates 30min, after refluxing extraction 1.5h,
It filters, concentration is settled to 500mL, takes concentrate, 50% 5 times of dilution in acetonitrile, filter membrane, sample introduction.
Water carries：Accurately weighing root of bidentate achyranthes 12g, Radix Angelicae Pubescentis 9g, Radix Angelicae Sinensis 6g, Rhizoma Et Radix Notopterygii 6g, windproof 6g, Radix Glycyrrhizae 6g is placed in round-bottomed flask
In, Extracting temperature is fixed as 80 DEG C, extracts 10 times of multiple, impregnates 30min, and after refluxing extraction 1.5h, 95% ethyl alcohol, which is added, makes second
Determining alcohol is 70%, one night of alcohol precipitation, is filtered, and concentration is settled to 500mL, takes concentrate, 50% 5 times of dilution in acetonitrile, filter membrane, into
Sample.
2.2.3 sample measures
According to method under " 2.1 " item, according to the retention time of reference substance, the retention time of each ingredient in sample is determined, product
Get each Component peak area, substitute into reference substance peak area and reference substance concentration, calculate the concentration of sample, and then calculates each
The content of a each ingredient of sample.
2.3 alcohol extracting taking techniques
2.3.1 volume fraction of ethanol preliminary examinations
It is Extraction solvent to select 30%, 50%, 70% and 90% ethyl alcohol respectively, and extraction multiple is 10 times, refluxing extraction 1 time
1.5h obtains extracting solution, and measuring flourish muscle according to test sample preparation method under method " 2.2.2 " item and " under 2.1 " chromatographic process picks up
β-ecdysterone, ferulic acid, Osthole, macrotin glycosides, Notopterol, Isomperatorin, the 5-O- methyl Wei Sia meter of pain side
The content of alcohol glycosides, glycyrrhizic acid investigates ethyl alcohol Extraction solvent and picks up the influence that pain side extracts to flourish muscle, from the figure 3, it may be seen that in 30%-
Between 50% concentration of alcohol, flourish muscle is picked up pain side's extraction result response and is not substantially change, but is carried from 50%-70% concentration of alcohol
It taking, concentration of alcohol increases, and extraction result response improves, and to 90% concentration of alcohol, response declines instead.For synthesis
70% proportion of ethanol response highest, therefore select 50%, 70%, 90% to be selected as orthogonal experiment concentration of alcohol when orthogonal experiment
The level selected.
2.3.2 extraction times amount preliminary examinations
Selective extraction multiple is 5,10,15 and 20 respectively, and 70% ethyl alcohol is Extraction solvent, and 1 1.5h of refluxing extraction is obtained
Extracting solution measures the β-that flourish muscle picks up pain side according to test sample preparation method under method " 2.2.2 " item and " under 2.1 " chromatographic process
Ecdysterone, ferulic acid, Osthole, macrotin glycosides, Notopterol, Isomperatorin, 5-O- methyl visamminols glycosides, Radix Glycyrrhizae
The content of acid, as a result such as Fig. 4, at 5-10 times, flourish muscle picks up pain side and extracts result response to be increased with the increase of solid-liquid ratio, and 15
Again, 20 times of significantly lower than 10 times amounts, therefore select 8,10 and 12 times of levels for measuring investigation again as orthogonal experiment.
2.3.3 extraction time preliminary examinations
Extracting 1,1.5,2 and 2.5h respectively, 70% ethyl alcohol is Extraction solvent, and extraction multiple is 10 times, refluxing extraction 1 time
To extracting solution, measures flourish muscle according to test sample preparation method under method " 2.2.2 " item and " under 2.1 " chromatographic process and pick up pain side
It is β-ecdysterone, ferulic acid, Osthole, macrotin glycosides, Notopterol, Isomperatorin, 5-O- methyl visamminols glycosides, sweet
The content of oxalic acid investigates extraction time in alcohol extracting and picks up the influence that pain side extracts result response to flourish muscle.As a result such as Fig. 5, with
The growth of extraction time, result response are gradually increasing, but response becomes in decline when extraction time is 2h to 2.5h
Gesture, while considering to save extraction time.Final choice 1h, 1.5h and 2h are orthogonal test inspection target.
2.3.4 extraction time preliminary examinations
70% ethyl alcohol is Extraction solvent, and solid-liquid ratio 1: 10, difference refluxing extraction 1,2,3 and 4 time, extraction time, 1.5h was obtained
To extracting solution, measures flourish muscle according to test sample preparation method under method " 2.2.2 " item and " under 2.1 " chromatographic process and pick up pain side
It is β-ecdysterone, ferulic acid, Osthole, macrotin glycosides, Notopterol, Isomperatorin, 5-O- methyl visamminols glycosides, sweet
The content of oxalic acid investigates alcohol extracting refluxing extraction number and picks up the influence that pain side extracts result response to flourish muscle.As a result it such as Fig. 6, carries
It takes number at 1 time less than extraction 2,3,4 times, and tends to balance when extraction reaches 2 times.Therefore 1,2 and 3 conduct of selection is orthogonal
Test the inspection target of extraction time.
2.3.5 alcohol extraction process orthogonal experiment
According to pertinent literature report and the experimental result of front, with β-ecdysterone, ferulic acid, Osthole, macrotin
Glycosides, Notopterol, Isomperatorin, 5-O- methyl visamminols glycosides, glycyrrhizic acid content be inspection target, extraction process scoring
Using total evaluation, computational methods：Overall score=∑ (maximum value of the recovery rate of each ingredient/each constituents extraction rate).It adopts
Pain side's alcohol extraction process orthogonal test is picked up with L16 (45) orthogonal arrage arrangement honor muscle, investigates volume fraction of ethanol, extraction times is measured, extraction
4 factors of number and extraction time, and variance analysis and significance test are carried out to result, determine that honor muscle is picked up the best of pain side and carried
Taking technique.Factor level table, orthogonal test calendar and analysis of variance table are as shown in the following table 1, table 2 and table 3.
1 each factor level table of orthogonal test alcohol extracting of table
3 alcohol extracting orthogonal experiments variance analysis of table
Note：F0.05(2,2)=19.
Variance analysis is carried out by error term of the B factors of very poor minimum it can be seen from variance analysis in table 1-3, in alcohol
It carries in technique, the maximum factor of influence of Extraction solvent, extraction four multiple, extraction time and extraction time factors to overall score
It is extraction time and times amount, the sequence for influencing the influence factor that flourish muscle picks up pain side's overall score is respectively：C > A > D > B, most preferably carry
Taking technique is A2B3C3D2, that is, 10 times, extraction time 2h of multiple is extracted, is extracted 3 times, 70% ethyl alcohol.
Beneficial effects of the present invention are proved below by way of the specific test of pesticide effectiveness.
The flourish muscle of 1 present invention of test example picks up the anti-inflammatory effect research of pain side
It is index to choose cell factor IL-1 β, IL-6, TNF-α, IL-10 below, anti-inflammatory to compare alcohol extract and water extract
Analgesic effect is good and bad.
The main pathological change of osteoarthritis (OA) is cartilage degeneration, shows as cartilage fibres, fracture, defect, dew
Go out subcartilaginous osseous lamella.The maintenance of articular cartilage structure and function is coordinated jointly dependent on both cartilage cell and matrix, and cartilage
Matrix normally synthesizes and decomposes the regulation and control of the dependent cells factor.Cell factor is common in inflammatory reaction, IL-1 β, IL-6, TNF-
The effect of α is typical inflammatory factor, and IL-10 can fight inflammatory factor.These cell factors and OA cartilage matrixs structure and work(
Energy, the physiological status of cartilage cell are closely related, or even can influence the structure and function of synovial membrane, subchondral bone.
1 experiment material
2 monthly age of 1.1 animal cleaning grade SD rats 30 come from Shanghai Slac Experimental Animal Co., Ltd., animal license
Card number is SCXK (Shanghai) 2012-0002.It raises in Fujian University of Traditional Chinese Medicine's Experimental Animal Center, experimental program meets medical faunae
The Experimental Ethical committee ratifies.
1.2 instrument Elx-800 microplate reader (Bio-Tek companies of the U.S.).
(biotech firm, article No. are built up in Nanjing to 1.3 reagent IL-1 β detection kits：MD6744), IL-6 detection kits
(biotech firm, article No. are built up in Nanjing：MD6746), (biotech firm, article No. are built up in Nanjing to TNF-α detection kit：MD6987),
(biotech firm, article No. are built up in Nanjing to IL-10 detection kits：MD6920).
2 experimental methods
2.1 modeling with grouping using random digits table by 30 rats be randomly divided into normal group, model group, control group,
Flourish muscle picks up pain side's water extract group (water puies forward group), flourish muscle picks up pain side's alcohol extract group (alcohol extracting group) (preparation of embodiment 2), totally 5 groups.Except just
Often group is outer, other group rats are all made of after 10% chloraldurate carries out intraperitoneal injection of anesthesia by every 0.3ml/100g and are improved
Hulth modus operandis establish knee OA models (HulthA, LindbergL, Telhag H.Experimental osteoarthritis
in rabbit.Preliminary report[J].ActaOrthop Scand, 1970,41 (5)：522-530.；Liu Xianxiang,
Li Xihai, Zhou Jiangtao improvement Hulth molding methods replicate the experimental study of knee osteoarthritis;J]Chinese combination of Chinese tradiational and Western medicine magazine,
2005,25 (12)：1104-1108.).
2.2 interference method rat dosages convert according to the equivalent dose ratio table that humans and animals body surface area is converted
(Huang Jihan, Huang Xiaohui, Chen Zhiyang wait the equivalent dose conversion between animal between animals and human beings body in pharmacological testings;J]Chinese
Clinical pharmacology and acology, 2004,9 (9)：1069-1072.) then control group give diclofenac sodium extended action tablet by 7.5mg
kg-1·d-1Amount carry out gavage, flourish muscle picks up pain side's water extract and alcohol extract equivalent dose by 4.5gkg-1·d-1Amount carry out
Gavage, model group and normal group give equal dosage physiological saline gavage.Daily gavage 1 time, continuous gavage 6 weeks.
After 2.3 materials last gavages, is pressed with 10% chloraldurate and carries out intraperitoneal injection of anesthesia per 0.3ml/100g,
Open abdominal cavity, the blood sampling of row abdominal aorta.Articular cavity is opened again, is sheared lower rat articular synovial tissue, is put in 1.5mL centrifuge tubes,
It is placed in liquid nitrogen container, stores for future use.Arterial blood centrifuges 15min after standing 2h under the conditions of 3000r/min, collects serum in 5mL
In centrifuge tube, it is put in rapidly in -80 DEG C of refrigerators, with to be detected.
Rat blood serum is taken out in 2.4ELISA detections, thaws under cryogenic conditions, in case experiment.Synovial tissue is then claimed with accurate
It takes 0.1g, is added phosphate buffer 1 mL, Ultrasonic Pulverization under cryogenic conditions, 12000r/min is centrifuged after fully shaking, takes supernatant
Liquid is in case experiment.According to gradient dilution method configuration standard product, after designing loading hole coordinate, be separately added into standard items, Quality Control and
Sample, concussion is uniform, and 37 DEG C of baking ovens are incubated 2 hours, automatic board-washing instrument board-washing 3 times, and antibody is added, and 37 DEG C of baking ovens are incubated.Automatically
Enzyme conjugates is added after board-washing instrument board-washing to be incubated, color developing agent is added, is eventually adding terminate liquid and terminates reaction, microplate reader 450nm waves
It is long to survey OD values.Calculate each sample IL-1 β, IL-6, TNF-α, IL-10 concentration.
3 results
Influence of the 3.1 each intervention groups to inflammatory cytokine in synovial tissue
3.1.1 influence of the different intervention groups to IL-1 β contents in synovial tissue
Compared with normal group, IL-1 β contents are significantly raised in model group, and difference is statistically significant (P=0.000).With
Model group is compared, and control group, water put forward group and alcohol extracting group IL-1 β contents and reduces, difference statistically significant (P=0.000, P
=0.000, P=0.000), illustrate that control group, water extract and alcohol extract can effectively adjust IL-1 β contents.A group phase is put forward with water
Than the reduction of alcohol extracting group IL-1 β contents becomes apparent from, and difference is statistically significant (P=0.000), illustrates that alcohol extract adjusts IL-1 β's
Curative effect is better than water extract.Compared with the control group, alcohol extracting group IL-1 β contents are substantially reduced, the statistically significant (P=of difference
0.000), illustrate that the effect of alcohol extract adjusts IL-1 β is better than control group.See Fig. 7.
Note：Compared with normal group, * * P < 0.05, * P < 0.01；Compared with model group,▲▲P < 0.05,▲P < 0.01；
Compared with water puies forward group,★★P < 0.05,★P < 0.01；Compared with the control group,□□P < 0.05,□P < 0.01.
3.1.2 influence of the different intervention groups to IL-6 contents in synovial tissue
Compared with normal group, IL-6 contents are significantly raised in model group, and difference is statistically significant (P=0.000).With mould
Type group is compared, and control group, water put forward group and alcohol extracting group IL-6 contents and reduces, difference statistically significant (P=0.000, P=
0.000, P=0.000), illustrate that control group, water extract and alcohol extract can effectively adjust IL-6 contents.Compared with water puies forward group, alcohol
It proposes a group IL-6 reductions to become apparent from, difference is statistically significant (P=0.000), illustrates that the effect of alcohol extract adjusts IL-6 is better than water
Extract.Compared with the control group, alcohol extracting group IL-6 contents are substantially reduced, and difference is statistically significant (P=0.000), illustrates alcohol extracting
Object adjusts the effect of IL-6 better than control group.See Fig. 8.
Note：Compared with normal group, * * P < 0.05, * P < 0.01；Compared with model group,▲▲P < 0.05,▲P < 0.01；
Compared with water puies forward group,★★P < 0.05,★P < 0.01；Compared with the control group,□□P < 0.05,□P < 0.01.
3.1.3 influence of the different intervention groups to TNF-α content in synovial tissue
Compared with normal group, TNF-α content is significantly raised in model group, and difference is statistically significant (P=0.000).With
Model group is compared, and control group, water put forward group and alcohol extracting group TNF-α content and reduces, difference statistically significant (P=0.000, P
=0.000, P=0.000), illustrate that control group, water extract and alcohol extract can effectively adjust TNF-α content.A group phase is put forward with water
Than alcohol extracting group TNF-α content is substantially reduced, and difference is statistically significant (P=0.000), illustrates that alcohol extract adjusts the treatment of TNF-α
Effect is better than water extract.Compared with the control group, alcohol extracting group TNF-α content is substantially reduced, and difference is statistically significant (P=0.000),
Illustrate that the effect of alcohol extract adjusts TNF-α is better than control group.See Fig. 9.
Note：Compared with normal group, * * P < 0.05, * P < 0.01；Compared with model group,▲▲P < 0.05,▲P < 0.01；
Compared with water puies forward group,★★P < 0.05,★P < 0.01；Compared with the control group,□□P < 0.05,□P < 0.01.
3.1.4 influence of the different intervention groups to IL-10 contents in synovial tissue
Compared with normal group, IL-10 contents are substantially reduced in model group, and difference is statistically significant (P=0.000).With
Model group is compared, and control group, water put forward group and alcohol extracting group IL-10 contents increase, difference statistically significant (P=0.000, P
=0.000, P=0.000), illustrate that control group, water extract and alcohol extract can effectively adjust IL-10 contents.A group phase is put forward with water
Than alcohol extracting group IL-10 contents are significantly raised, and difference is statistically significant (P=0.000), illustrate that alcohol extract adjusts the treatment of IL-10
Effect is better than water extract.Compared with the control group, alcohol extracting group IL-10 contents obviously increase, and difference is statistically significant (P=0.000),
Illustrate that the effect of alcohol extract adjusts IL-10 is better than control group.See Figure 10.
Note：Compared with normal group, * * P < 0.05, * P < 0.01；Compared with model group,▲▲P < 0.05,▲P < 0.01；
Compared with water puies forward group,★★P < 0.05,★P < 0.01；Compared with the control group,□□P < 0.05,□P < 0.01.
Influence of the 3.2 each intervention groups to serum inflammatory cell factor
3.2.1 influence of the different intervention groups to IL-1 β contents in serum
Compared with normal group, IL-1 β contents are significantly raised in model group, and difference is statistically significant (P=0.000).With
Model group is compared, and control group, water put forward group and alcohol extracting group IL-1 β contents and reduces, difference statistically significant (P=0.000, P
=0.000, P=0.000), illustrate that control group, water extract and alcohol extract can effectively adjust IL-1 β contents.A group phase is put forward with water
Than alcohol extracting group IL-1 β contents are substantially reduced, and difference is statistically significant (P=0.000), illustrate that alcohol extract adjusts the treatment of IL-1 β
Effect is better than water extract.Compared with the control group, alcohol extracting group IL-1 β contents reduce, and difference is statistically significant (P=0.004), explanation
Alcohol extract adjusts the effect of IL-1 β better than control group.See Figure 11.
Note：Compared with normal group, * * P < 0.05, * P < 0.01；Compared with model group,▲▲P < 0.05,▲P < 0.01；
Compared with water puies forward group,★★P < 0.05,★P < 0.01；Compared with the control group,□□P < 0.05,□P < 0.01.
3.2.2 influence of the different intervention groups to IL-6 contents in serum
Compared with normal group, IL-6 contents are significantly raised in model group, and difference is statistically significant (P=0.000).With mould
Type group is compared, and control group, water put forward group and alcohol extracting group IL-6 expression quantity and reduces, difference statistically significant (P=0.000, P
=0.000, P=0.000), illustrate that control group, water extract and alcohol extract can effectively adjust IL-6 contents.Compared with water puies forward group,
Alcohol extracting group IL-6 contents are substantially reduced, and difference is statistically significant (P=0.000), illustrate that the effect of alcohol extract adjusts IL-6 is excellent
In water extract.Compared with the control group, alcohol extracting group IL-6 contents reduce, and difference is statistically significant (P=0.001), illustrates alcohol extracting
Object adjusts the effect of IL-6 better than control group.See Figure 12.
Note：Compared with normal group, * * P < 0.05, * P < 0.01；Compared with model group,▲▲P < 0.05,▲P < 0.01；
Compared with water puies forward group,★★P < 0.05,★P < 0.01；Compared with the control group,□□P < 0.05,□P < 0.01.
3.2.3 influence of the different intervention groups to TNF-α content in serum
Compared with normal group, TNF-α content is significantly raised in model group, and difference is statistically significant (P=0.000).With
Model group is compared, and control group, water put forward group and alcohol extracting group TNF-α content and reduces, difference statistically significant (P=0.000, P
=0.000, P=0.000), illustrate that control group, water extract and alcohol extract can effectively adjust TNF-α content.A group phase is put forward with water
Than alcohol extracting group TNF-α content is substantially reduced, and difference is statistically significant (P=0.000), illustrates that alcohol extract adjusts the treatment of TNF-α
Effect is better than water extract.Compared with the control group, alcohol extracting group TNF-α content is substantially reduced, and difference is statistically significant (P=0.000),
Illustrate that the effect of alcohol extract adjusts TNF-α is better than control group.See Figure 13.
Note：Compared with normal group, * * P < 0.05, * P < 0.01；Compared with model group,▲▲P < 0.05,▲P < 0.01；
Compared with water puies forward group,★★P < 0.05,★P < 0.01；Compared with the control group,□□P < 0.05,□P < 0.01.
3.2.4 influence of the different intervention groups to IL-10 contents in serum
Compared with normal group, IL-10 contents are substantially reduced in model group, and difference is statistically significant (P=0.000).With
Model group is compared, and control group, water put forward group and alcohol extracting group IL-10 contents increase, difference statistically significant (P=0.000, P
=0.000, P=0.000), illustrate that control group, water extract and alcohol extract can effectively adjust IL-10 contents.A group phase is put forward with water
Than alcohol extracting group IL-10 contents are significantly raised, and difference is statistically significant (P=0.000), illustrate that alcohol extract adjusts the treatment of IL-10
Effect is better than water extract.Compared with the control group, alcohol extracting group IL-10 contents are significantly raised, and difference is statistically significant (P=0.000),
Illustrate that the effect of alcohol extract adjusts IL-10 is better than control group.See Figure 14.
Note：Compared with normal group, * * P < 0.05, * P < 0.01；Compared with model group,▲▲P < 0.05,▲P < 0.01；
Compared with water puies forward group,★★P < 0.05,★P < 0.01；Compared with the control group,□□P < 0.05,□P < 0.01.
Conclusion：
Alcohol extract and water extract can effectively reduce IL-1 β, IL-6, TNF-α content, improve IL-10 contents, especially alcohol
Extract is better than water extract to the adjustment effect of these inflammatory factors, and alcohol extract curative effect is better than control group.
Claims (8)
1. a kind of preparation method of the pharmaceutical composition for the treatment of of arthritis, it is characterised in that：It includes the following steps：
A, the bulk pharmaceutical chemicals of each weight proportion are weighed：
10~14 parts of the root of bidentate achyranthes, 4~8 parts of Radix Angelicae Sinensis, 6~12 parts of Radix Angelicae Pubescentis, 4~8 parts of Rhizoma Et Radix Notopterygii, 4~8 parts windproof, 4~8 parts of Radix Glycyrrhizae；
B, 8~12 times of a concentration of 50%~90% ethyl alcohol of amount are added, impregnates 20~40min, is heated to 80~85 DEG C, extraction time
1~2h, extract 1~3 time, filtering, concentrate to get.
2. preparation method according to claim 1, it is characterised in that：The weight proportion of bulk pharmaceutical chemicals described in a steps is：
12 parts of the root of bidentate achyranthes, 6 parts of Radix Angelicae Sinensis, 9 parts of Radix Angelicae Pubescentis, 6 parts of Rhizoma Et Radix Notopterygii, 6 parts windproof, 6 parts of Radix Glycyrrhizae.
3. preparation method according to claim 1 or 2, it is characterised in that：The addition of ethyl alcohol described in b step is 10 times
Amount.
4. preparation method according to claim 1 or 2, it is characterised in that：The concentration of alcohol is 70% ethyl alcohol.
5. preparation method according to claim 1 or 2, it is characterised in that：The dip time is 30min.
6. preparation method according to claim 1 or 2, it is characterised in that：The heating temperature is 80 DEG C.
7. extracting method according to claim 1 or 2, it is characterised in that：The extraction time is 2h.
8. extracting method according to claim 1 or 2, it is characterised in that：The extraction time is 3 times.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103316140A (en) * | 2013-06-24 | 2013-09-25 | 周博 | Drug for treating arthrophlogosis |
| CN106389573A (en) * | 2016-11-16 | 2017-02-15 | 陕西玉航电子有限公司 | Rheumatism liquid for curing gonitis |
| CN106974958A (en) * | 2017-04-26 | 2017-07-25 | 福建中医药大学 | Flourish muscle picks up pain side and its production and use |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103316140A (en) * | 2013-06-24 | 2013-09-25 | 周博 | Drug for treating arthrophlogosis |
| CN106389573A (en) * | 2016-11-16 | 2017-02-15 | 陕西玉航电子有限公司 | Rheumatism liquid for curing gonitis |
| CN106974958A (en) * | 2017-04-26 | 2017-07-25 | 福建中医药大学 | Flourish muscle picks up pain side and its production and use |
Non-Patent Citations (3)
| Title |
|---|
| 尹莲: "《天然药物化学 新世纪第2版》", 31 July 2017, 中国中医药出版社 * |
| 武子敬等: "《中药化学》", 30 June 2017, 电子科技大学出版社 * |
| 郑春松等: "计算机模拟研究荣筋拈痛方治疗骨关关节炎的药效物质基础、作用靶点及作用特点", 《中医正骨》 * |
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