CN108703251A - A method of preparing edible albumen and carbohydrate using biomass - Google Patents

A method of preparing edible albumen and carbohydrate using biomass Download PDF

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Publication number
CN108703251A
CN108703251A CN201810227927.7A CN201810227927A CN108703251A CN 108703251 A CN108703251 A CN 108703251A CN 201810227927 A CN201810227927 A CN 201810227927A CN 108703251 A CN108703251 A CN 108703251A
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China
Prior art keywords
carbohydrate
biomass
method preparing
albumen
edible albumen
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CN201810227927.7A
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Chinese (zh)
Inventor
杨宗鑫
陈春辉
周敏
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Hangzhou Xinwei Low Carbon Technology R & D Co Ltd
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Hangzhou Xinwei Low Carbon Technology R & D Co Ltd
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Priority to CN201810227927.7A priority Critical patent/CN108703251A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/008Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Disclosed by the invention is a kind of method preparing edible albumen and carbohydrate using biomass, preparation method includes the removal impurity of biomass, pretreatment, saccharification, liquid state fermentation, extraction, precipitate and decolorization taste removal cleans, it is dry, safety testing, the pretreatment is that the biomass after removal impurity is cut into segment or is crushed into powder, pretreated biomass is saccharified and obtains saccharified liquid through high steam explosion or acid/base processing or bacterium degradation or enzymatic treatment or combinations thereof, again through precipitation, decoloration, taste removal, decontamination, the dry carbohydrate that solid particle is made, or saccharification formula of liquid is obtained into protide through liquid state fermentation and subsequent processing, the practice and extension of the present invention can provide endlessly edible albumen and carbohydrate for the mankind, reduce cultivated land occupancy, turn waste into wealth, reduce pollution, be conducive to national food security strategy and environmental protection.

Description

A method of preparing edible albumen and carbohydrate using biomass
Technical field
The present invention relates to a kind of methods preparing edible albumen and carbohydrate, more specifically say, are related to a kind of utilize and give birth to The method that substance prepares edible albumen and carbohydrate.
Background technology
For green plants by photosynthesis continuously synthesising biological matter resource, the major part in them cannot be by the mankind It eats, this is primarily due to them and all contains more or less lignin, forms hard cell wall.With economic society Development, the mankind are increasing to the demand of edible albumen and carbohydrate, and people daily required edible albumen and carbohydrate Greatly mostly from industrial crops, they occupy cultivated land, limits throughput, or even rely on import, need to expend a large amount of resource and fund, The present invention, for raw material, is prepared edible with the low awns straw or like vegetable of economic value, reed class plant, stalk, bagasse, garbage etc. With albumen and carbohydrate, it will reduce cultivated land occupancy, reduce environmental pollution, reduce the wasting of resources, improve biomass money in nature The utilization ratio in source is conducive to national food security strategy.
Invention content
The purpose of the present invention is to provide a kind of method preparing edible albumen and carbohydrate using biomass, party's legal systems Standby edible albumen and carbohydrate, the utilization ratio that can be reduced waste, improve biomass resource, provide endlessly for the mankind Edible albumen and carbohydrate, it is edible etc. for industrial production, animal feed or the mankind.
A method of preparing edible albumen and carbohydrate, including following preparation process using biomass:
Step 1):Biomass is placed on mesh screen and washes off impurity with giant, juxtaposition air-dries spare at room temperature;
Step 2):0.5-5cm segments are cut into the rear biomass pretreatment of step 1) processing or are crushed into powder;
Step 3):The pretreated biomass of step 2) is subjected to cellulose processing, then is saccharified, saccharified liquid is obtained, Be formulated by saccharified liquid precipitate and decolorization taste removal removal of impurities, the dry carbohydrate that solid particle is made, or by saccharified liquid, be added strain into Row liquid state fermentation, then precipitation, removal of impurities of decolourizing, dry, acquisition mycelium, protide is obtained by mycelium processing;
Step 4):The carbohydrate or protide that step 3) is obtained carry out toxicological experiment, zoopery, human experimentation, obtain Edible carbohydrates or edible albumen.
As an improvement the biomass includes awns straw or like vegetable, reed class plant, stalk, bagasse, kitchen biology rubbish Rubbish.
As an improvement the strain includes whiterot fungi, brown rot fungus, soft-rot fungi.
As an improvement during the cellulose processing is high steam explosion, acid or alkali process, fungi degradation and digests It is any one or more.
As an improvement the explosion temperature of the high steam explosion is 150-220 DEG C, reaction time 5- 45min。
As an improvement the enzyme includes lignoenzyme, hemilignin enzyme, cellulase, hemicellulase.
As an improvement containing the sources N in the formula, the sources N include thiamines, urea, ammonium hydrogen carbonate, animal leftover bits and pieces Material.
As an improvement the protide includes protein, amino acid and peptides.
As an improvement the carbohydrate includes polysaccharide, disaccharide, monosaccharide.
Advantageous effect:The practice and extension of the present invention can provide endlessly edible albumen and carbohydrate for the mankind, reduce Cultivated land occupancy is turned waste into wealth, and pollution is reduced, and is conducive to national food security strategy and environmental protection;Prepare simple, operation side Just, be conducive to produce in batches;It is environmentally protective, pollution-free.
Description of the drawings
Fig. 1 is flow diagram of the present invention
Specific implementation mode
Below by way of specific embodiment, the present invention is further illustrated, but embodiments of the present invention not by The limitation of following embodiment.
Embodiment 1
A method of preparing edible albumen and carbohydrate, including following preparation process using biomass:
Step 1):Biomass is placed on mesh screen and washes off impurity with giant, juxtaposition air-dry at room temperature it is spare, The biomass includes awns straw or like vegetable, reed class plant, stalk (paddy and wheat, sorghum, corn), bagasse, kitchen biologic garbage;
Step 2):0.5cm segments are cut into the rear biomass pretreatment of step 1) processing or are crushed into powder;
Step 3):The pretreated biomass of step 2) is subjected to cellulose processing, then is saccharified, saccharified liquid is obtained, Saccharified liquid precipitate and decolorization, taste removal removal of impurities, drying are made the carbohydrate of solid particle, or take and be formulated saccharified liquid, is added Strain carries out liquid state fermentation, then precipitates (precipitating mycelium), decoloration removal of impurities (mycelium is detached with impurity), drying, obtains Mycelium is processed and obtains protide by mycelium, and the cellulose processing is high steam explosion, acid or alkali process, the height It is 150 DEG C to press the explosion temperature of steam blasting, is depressured rapidly after reacting 5min, and indirect steam is generated so that volume surges, raw Materials Cell wall is destroyed, and lignin and cellulose separation, hemicellulose is hydrolyzed, obtains saccharified liquid, the formula is will be sugared Change liquid and wood fibre enzyme and cellulase enzymolysis is added, contains the sources N in formula, the sources N include under thiamines, urea, ammonium hydrogen carbonate, animal Heel, the strain that liquid state fermentation uses include whiterot fungi, brown rot fungus, soft-rot fungi;
Step 4):The carbohydrate or protide that step 3) is obtained carry out toxicological experiment, zoopery, human experimentation, obtain Edible carbohydrates or edible albumen, the protide include protein, amino acid and peptides, and the carbohydrate includes polysaccharide, double Sugar, monosaccharide.
Embodiment 2
A method of preparing edible albumen and carbohydrate, including following preparation process using biomass:
Step 1):Biomass is placed on mesh screen and washes off impurity with giant, juxtaposition air-dry at room temperature it is spare, The biomass includes awns straw or like vegetable, reed class plant, stalk (paddy and wheat, sorghum, corn), bagasse, kitchen biologic garbage;
Step 2):3cm segments are cut into the rear biomass pretreatment of step 1) processing or are crushed into powder;
Step 3):The pretreated biomass of step 2) is subjected to cellulose processing, then is saccharified, saccharified liquid is obtained, The carbohydrate of solid particle is made in saccharified liquid precipitate and decolorization taste removal removal of impurities, drying, or takes and is formulated saccharified liquid, bacterium is added Kind carries out liquid state fermentation, and then precipitation, removal of impurities of decolourizing, drying, obtain mycelium, and mycelium processing is obtained protide, described Cellulose processing is high steam explosion, fungi degradation, and the explosion temperature of the high steam explosion is 185 DEG C, after reacting 25min Rapid decompression, generates indirect steam so that volume surges, and biomass cells wall is destroyed, lignin and cellulose separation, half fiber Dimension element is hydrolyzed, obtains saccharified liquid, and the formula is that wood fibre enzyme and cellulase enzymolysis is added in saccharified liquid, is contained in formula The sources N, the sources N include thiamines, urea, ammonium hydrogen carbonate, tankage, the strain that liquid state fermentation uses include whiterot fungi, brown rot fungus, Soft-rot fungi;
Step 4):The carbohydrate or protide that step 3) is obtained carry out toxicological experiment, zoopery, human experimentation, obtain Edible carbohydrates or edible albumen, the protide include protein, amino acid and peptides, and the carbohydrate includes polysaccharide, double Sugar, monosaccharide.
Embodiment 3
A method of preparing edible albumen and carbohydrate, including following preparation process using biomass:
Step 1):Biomass is placed on mesh screen and washes off impurity with giant, juxtaposition air-dry at room temperature it is spare, The biomass includes awns straw or like vegetable, reed class plant, stalk (paddy and wheat, sorghum, corn), bagasse, kitchen biologic garbage;
Step 2):5cm segments are cut into the rear biomass pretreatment of step 1) processing or are crushed into powder;
Step 3):The pretreated biomass of step 2) is subjected to cellulose processing, then is saccharified, saccharified liquid is obtained, The carbohydrate of solid particle is made in saccharified liquid precipitate and decolorization taste removal removal of impurities, drying, or takes and is formulated saccharified liquid, bacterium is added Kind carries out liquid state fermentation, and then precipitation, removal of impurities of decolourizing, drying, obtain mycelium, and mycelium processing is obtained protide, described Cellulose processing is high steam explosion, fungi degradation, and the explosion temperature of the high steam explosion is 220 DEG C, after reacting 45min Rapid decompression, generates indirect steam so that volume surges, and biomass cells wall is destroyed, lignin and cellulose separation, half fiber Dimension element is hydrolyzed, obtains saccharified liquid, and the formula is that wood fibre enzyme and cellulase enzymolysis is added in saccharified liquid, is contained in formula The sources N, the sources N include thiamines, urea, ammonium hydrogen carbonate, tankage, and the strain that liquid state fermentation uses includes whiterot fungi, brown Rotten bacterium, soft-rot fungi;
Step 4):The carbohydrate or protide that step 3) is obtained carry out toxicological experiment, zoopery, human experimentation, obtain Edible carbohydrates or edible albumen, the protide include protein, amino acid and peptides, and the carbohydrate includes polysaccharide, double Sugar, monosaccharide.
Embodiment 4
A method of preparing edible albumen and carbohydrate, including following preparation process using biomass:
Step 1):Biomass is placed on mesh screen and washes off impurity with giant, juxtaposition air-dry at room temperature it is spare, The biomass includes awns straw or like vegetable, reed class plant, stalk (paddy and wheat, sorghum, corn), bagasse, kitchen biologic garbage;
Step 2):5cm segments are cut into the rear biomass pretreatment of step 1) processing or are crushed into powder;
Step 3):The pretreated biomass of step 2) is subjected to cellulose processing, then is saccharified, saccharified liquid is obtained, The carbohydrate of solid particle is made in saccharified liquid precipitate and decolorization taste removal removal of impurities, drying, or takes and is formulated saccharified liquid, bacterium is added Kind carries out liquid state fermentation, and then precipitation, removal of impurities of decolourizing, drying, obtain mycelium, and mycelium processing is obtained protide, described Cellulose processing is enzymolysis, obtains saccharified liquid, and the formula is that wood fibre enzyme and cellulase enzymolysis, formula is added in saccharified liquid In contain the sources N, the sources N include thiamines, urea, ammonium hydrogen carbonate, tankage, the strain that liquid state fermentation uses include whiterot fungi, Brown rot fungus, soft-rot fungi;
Step 4):The carbohydrate or protide that step 3) is obtained carry out toxicological experiment, zoopery, human experimentation, obtain Edible carbohydrates or edible albumen, the protide include protein, amino acid and peptides, and the carbohydrate includes polysaccharide, double Sugar, monosaccharide.
Finally it should be noted that present invention is not limited to the above embodiments, there can also be many variations.This field it is general All deformations that logical technical staff directly can export or associate from present disclosure are considered as the present invention's Protection domain.

Claims (9)

1. a kind of method preparing edible albumen and carbohydrate using biomass, it is characterised in that including following preparation process:
Step 1):Biomass is placed on mesh screen and washes off impurity with giant, juxtaposition air-dries spare at room temperature;
Step 2):0.5-5cm segments are cut into the rear biomass pretreatment of step 1) processing or are crushed into powder;
Step 3):The pretreated biomass of step 2) is subjected to cellulose processing, then is saccharified, saccharified liquid is obtained, it will be sugared Change liquid precipitate decoloration, taste removal removal of impurities, drying, the carbohydrate of solid particle is made;Or be formulated saccharified liquid, strain is added and carries out Liquid state fermentation, then precipitate and decolorization, removal of impurities, drying obtain mycelium, and mycelium is processed and obtains protide;
Step 4):The carbohydrate or protide that step 3) is obtained carry out toxicological experiment, zoopery, human experimentation, obtain edible With carbohydrate or edible albumen.
2. a kind of method preparing edible albumen and carbohydrate using biomass according to claim 1, which is characterized in that The biomass includes awns straw or like vegetable, reed class plant, stalk, bagasse, kitchen biologic garbage.
3. a kind of method preparing edible albumen and carbohydrate using biomass according to claim 1, which is characterized in that The strain includes whiterot fungi, brown rot fungus, soft-rot fungi.
4. a kind of method preparing edible albumen and carbohydrate using biomass according to claim 1, which is characterized in that The cellulose processing is high steam explosion, acid or alkali process, fungi degradation and enzymolysis any one or more of.
5. a kind of method preparing edible albumen and carbohydrate using biomass according to claim 4, which is characterized in that The explosion temperature of the high steam explosion is 150-220 DEG C, reaction time 5-45min.
6. a kind of method preparing edible albumen and carbohydrate using biomass according to claim 4, which is characterized in that The enzyme includes lignoenzyme, hemilignin enzyme, cellulase, hemicellulase.
7. a kind of method preparing edible albumen and carbohydrate using biomass according to claim 1, which is characterized in that Contain the sources N in the formula, the sources N include thiamines, urea, ammonium hydrogen carbonate, tankage.
8. a kind of method preparing edible albumen and carbohydrate using biomass according to claim 1, which is characterized in that The protide includes protein, amino acid and peptides.
9. a kind of method preparing edible albumen and carbohydrate using biomass according to claim 1, which is characterized in that The carbohydrate includes polysaccharide, disaccharide, monosaccharide.
CN201810227927.7A 2018-03-20 2018-03-20 A method of preparing edible albumen and carbohydrate using biomass Pending CN108703251A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109576729A (en) * 2019-01-28 2019-04-05 谢吉萍 A kind of preparation method of no acid alkali metal degreaser
CN109748309A (en) * 2019-02-25 2019-05-14 谭祖伍 A kind of preparation method of high dispersion of nano-calcium carbonate
CN110004495A (en) * 2019-02-20 2019-07-12 谭凤香 A kind of method of monocrystalline silicon making herbs into wool

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101998995A (en) * 2008-04-11 2011-03-30 帝国创新有限公司 Methods of pretreatment of cellulosic or lignocellulosic biomass

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101998995A (en) * 2008-04-11 2011-03-30 帝国创新有限公司 Methods of pretreatment of cellulosic or lignocellulosic biomass

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
庄桂: "利用玉米秸秆生产酵母蛋白的研究", 《郑州工程学院学报》 *
瞿国才: "蔗糖蛋白饲料的生产工艺", 《河南农业》 *
贠建民,等: "马铃薯淀粉渣生料多菌种固态发酵生产蛋白饲料工艺", 《农业工程学报》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109576729A (en) * 2019-01-28 2019-04-05 谢吉萍 A kind of preparation method of no acid alkali metal degreaser
CN110004495A (en) * 2019-02-20 2019-07-12 谭凤香 A kind of method of monocrystalline silicon making herbs into wool
CN109748309A (en) * 2019-02-25 2019-05-14 谭祖伍 A kind of preparation method of high dispersion of nano-calcium carbonate

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Application publication date: 20181026