CN1086942C - Application of butyl phthalide in preparing medicines curing thrombosis and thrombocyte coagulation - Google Patents
Application of butyl phthalide in preparing medicines curing thrombosis and thrombocyte coagulation Download PDFInfo
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- CN1086942C CN1086942C CN98125618A CN98125618A CN1086942C CN 1086942 C CN1086942 C CN 1086942C CN 98125618 A CN98125618 A CN 98125618A CN 98125618 A CN98125618 A CN 98125618A CN 1086942 C CN1086942 C CN 1086942C
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Abstract
The present invention relates to application of butylphthalide to the preparation of medicines for treating thrombosis and antiplatelet aggregation. The butylphthalide is levorotatory in an optimized executive plan.
Description
The present invention relates to the new purposes of butylphthalide (dl-3-n-butylphthalide is called for short NBP), more specifically relate to the application of this chemical compound in anti-thrombosis drug and medicament for resisting platelet aggregation.
Cardiovascular and cerebrovascular disease such as atherosclerosis, hypertension etc., easily cause tunica intima impaired, slow blood flow, it is big that blood viscosity becomes, blood is in high sticking, Gao Ning, high state (StevensMK and Yaksh TL, the Time course of release in vivo of PGE of gathering
2, PGF
2 α, 6-keto-PGF
1 α, and TXB
2Into the brain extracellular space after 15min of complete global ischemia in the presence and absence ofcyclooxygenase inhibition, J cereb blood flow metab 1988,8:790-94; And Wu JF, Liu TP, Effects of berberine on plateletaggregation and plasma levels of TXB
2And 6-keto-PGF
1 αIn rats withreversible middle cerebral artery occlusion, Acta Pharm Sin 1995,30 (2): 98-102).As seen, antithrombotic therapy is significant in control thrombosis cerebrovascular disease.
Butylphthalide has effect (Chong ZZ and Feng YP, the Effects of dl-3-n-butylphthalide on production of TXB that regulates neurocyte arachidonic acid metabolic after NOS-NO-cGMP systemic-function and the cerebral ischemia
2And 6-keto-PGF
1 αIn rat brainduring focal cerebral ischemia and reperfusion, Acta PharmacolSin, 1997,18:505-08; And Yan CH and Feng YP, Effects of d-3-n-butylphthalide and 1-3-n-butylphthalide extracellular NO leveland intracellular cGMP level in primary cultured rat corticalneurons, Acta Pharm Sin, 1998,33 (6): 418-423).And the generation of arachidonic acid metabolic and NO has significant effects to thrombosis, platelet function and the hemodynamics of body.
Therefore, the object of the present invention is to provide effect of butylphthalide anticoagulant and the application in the preparation anti-thrombosis drug.
Butylphthalide is a kind of chipal compounds, and it comprises racemization, left-handed and dextrorotation butylphthalide, is abbreviated as dl-, l-and d-NBP respectively.
One aspect of the present invention comprises the application of NBP in anti-thrombosis drug.Preferably, described butylphthalide is l-NBP.
The present invention comprises the application of NBP in anti-thrombosis drug on the other hand.Preferably, described butylphthalide is l-NBP.
NBP can the pure material form or with materia medica on acceptable excipient or carrier combinations administration.For the dosage form of described excipient or carrier and compositions, but those skilled in the art's reference standard teaching material, as Remington ' s Pharmaceutical Sciences 17
ThVersion is determined.The dosage of NBP or its compositions depends on following factor: the order of severity of disease to be treated, route of administration and patient's sex, age, health status etc., and this is conspicuous to common resident doctor.And those of ordinary skills can easily determine suitable dosage, mode and frequency.In general, behind the oral l-NBP 200mg, the platelet aggregation rate that ADP and AA cause is starkly lower than normally (P＜0.01 or P＜0.05), and has certain time-effect relationship.The drug effect of l-NBP begins to weaken after continuing to 4 hours, and it is reversible pointing out out the l-NBP effect, and this is very useful to data for clinical drug use.
NBP or its compositions can be used for the formation of anticoagulant and thrombosis, control apoplexy, coronary heart disease and peripheral blood vessel.At acute stage and convalescent period cerebral infarction the clinical II phase test, through at random, double blinding, placebo-controlled trial, find that NBP group (n=25) compares with placebo group (n=25) that there were significant differences (P＜0.01).This shows that NBP has good therapeutic effect to acute ischemic cerebral apoplexy.
Thrombosis is one of main paathogenic factor in the ischemic cardiovascular and cerebral vascular disease.And during cerebral ischemia, PGI in the brain
2/ TXA
2Ratio obviously descend, simultaneously, platelet function abnormality is hyperfunction, blood is in hypercoagulability, this is one of major reason that causes secondary brain injury.The follow-up embodiment of the present invention utilizes rat carotid artery-external jugular vein blood flow the method for bypass, has observed the influence of the NBP of different optical activitys to the formation of half external thrombus, and compares with Asp.The result shows that ip administration dl and l-NBP all have tangible anti-thrombosis function, and action effect is weaker than Asp when low dose of, and during increased dosage amount, the effect of l-NBP then is better than Asp.After irritating stomach (ig) administration, dl-NBP and l-NBP can also reduce wet weight of thrombus in dose dependent ground, but than a little less than the Asp with dosage, l-NBP and Asp low dosage administering drug combinations, its thromboembolism preventing effect obviously strengthens, and is similar to the l-NBP or the independent result of use of Asp of high dose.In a word, NBP has thromboembolism preventing effect in the stronger body, irritates the stomach effect and obviously is weaker than intraperitoneal injection, and this may be low relevant with bioavailability of medicament behind the filling stomach.
Because the platelet function activation is hyperfunction to be thrombotic key factor, so further study the influence of NBP to platelet function.Found that, but dl-, d-and l-NBP all dose dependent ground inhibition AA, collagen (Coll), the inductive platelet aggregation of ADP, and the platelet aggregation that when higher concentration AA is caused has relative selectivity, and wherein the l-NBP effect is the strongest.The level of dl-NBP and the interior cAMP of l-NBP energy dose dependent platelet increasing, and cAMP can bring into play antiplatelet effects by suppressing the interior calcium ion concentration rising of endochylema in the platelet.
In addition, TXA
2Also be the informational molecule that has important physiological function in the platelet, AA produces TXA through epoxidase, thromboxane synthetase metabolism on the platelet membrane phospholipid
2, it can directly activate PLC in feedback ground, reduces cAMP level and induced platelet gathering (modern thrombotic disease is learned, Beijing: Beijing Medical University, combined publication society of China Concord Medical Science University, 1997, the 508-509 pages or leaves for Wang Zhong, Zheng Zhiquan chief editor) in the platelet.Zhong Zhaozhong etc. discover people such as (, the same) Chong ZZ, and dl-, d-and l-NBP all can dose dependent ground suppress that AA discharges in the ischemic tissue of brain, and promotion cerebral cortex cell PGI
2Synthetic, suppress TXA
2Synthetic, thereby rising PGI
2/ TXA
2Ratio, and think one of the main mechanism that the AA metabolism is its performance pharmacological action that influences.
And find the platelet T XA that dl-, d-NBP cause exogenous AA in the present invention's research
2Increasing does not all have obviously influence, and l-NBP is also only in high concentration (10
-4More weak inhibitory action is arranged in the time of mol/L).Simultaneously, current research found that, dl-, l-NBP can dose dependent rising endothelial cells cultured in PGI
2Discharge, and d-NBP is to PGI
2Generation do not have obvious effect.As seen, influence the AA metabolism, especially increase PGI
2Generation, in the anti thrombotic action of NBP, play an important role, also be the l-NBP effect be better than d-NBP may mechanism one of.
Result of the present invention also finds, and is different with the Asp effect, and l-NBP can suppress significantly to have in the platelet that induced platelet is assembled and the release of the 5-HT of blood vessel contracture effect, and dl-, d-NBP, metabolism does not make significant difference Asp to 5-HT.
In sum, dl-, but d-and l-NBP suppress hematoblastic function on external all dose dependent ground, and the most remarkable with l-NBP.In the thromboembolism preventing experiment, also remarkable in the body with the l-NBP effect.L-NBP has the platelet of inhibition 5-HT and discharges, and the effect of cAMP level in the increased platelets counts is the important component of dl-NBP performance anti thrombotic action.As and if d-NBP does not have the thromboembolism preventing effect, may there be stereo selectivity in this with biomacromolecule in the body, makes that two kinds of optical isomers metabolic pathway and generation activated product difference in vivo is relevant.
This result of study shows that NBP has anti thrombotic action, the research of prompting NBP and use the prevention of thrombosis cerebral ischemia disease and peripheral blood vessel embolism class diseases and treat significant.
Describe the present invention in detail below with reference to drawings and Examples, so that objects and advantages of the present invention will be more obvious.In the accompanying drawings:
What Fig. 1 showed is the effect that NBP and Asp (ip) form experimental thrombosis, and wherein, each organizes n=6,
*P＜0.05,
*Compare with group of solvents P＜0.001.
What Fig. 2 showed is the effect that NBP and Asp (ip) form experimental thrombosis, and wherein, each organizes n=6,
*P＜0.05,
*P＜0.01,
* *Compare with group of solvents P＜0.001.
What Fig. 3 showed is the effect that NBP and Asp discharge 5-HT in the platelet, and wherein, each organizes n=6,
*P＜0.05,
*P＜0.01,
* *Compare with group of solvents P＜0.001.
What Fig. 4 showed is the effect of 1-NBP to the inductive human platelet aggregation of ADP.
What Fig. 5 showed is the effect of 1-NBP to the inductive human platelet aggregation of AA.
To use among the following embodiment: the material animal
Sprague-Dawley rat and rabbit are provided by Ministry of Public Health medicine calibrating institute Animal House.Medicine and reagent
Dl-, l-and d-NBP are provided by institute of Materia Medica,Chinese Academy of Medical Sciences professor Yang Jinghua, purity＞96%, optical rotation be respectively 0 ,-69.73 and+64.08 the degree.(Aspirin Asp) is pharmaceutical factory of Xinhua product to aspirin; (collagen Coll) makes by oneself with rat skin collagen; Thrombin is The Biochemical Pharmaceutical Factory of Zhuhai SEZ production; Adenosine diphosphate (ADP) is available from Sigma company; Arachidonic acid (AA), o-phthalaldehyde(OPA) (OPT) are available from Fluka company.OPT faces with preceding and is dissolved as 0.004% solution with HCl (10mol/L), puts 4 ℃ of refrigerators and preserves.CAMP, TXB
2The ria-determination test kit is respectively available from Institute for Atomic Research, Beijing and East Asia, Beijing technical research institute.Instrument
TYXN-91 intelligence platelet aggregation instrument (Shanghai General Machinery ﹠ Electric technology Inst.'s production).
Platelet aggregation instrument (a day island proper Tianjin produces).The statistical analysis method
Experimental result is with mean+SD (SE) expression, and with the significance of t check analysis difference.Embodiment 1:dl-, l-and d-NBP are to the thrombotic influence of rat experiment
Rat, body weight are between 260-300g, and be male, random packet, every group of 6 animals.Intraperitoneal administration group (ip): Asp, dl-, d-and l-NBP (dosage be respectively 5,10,20mg/kg) and with the normal saline (NS) of volume.Behind 20min with thrombosis the method for bypass (UmetzuT that experimentizes, Sanai K, Effect of 1-Methyl-2-Mercapto-5-(3-Pyridyl)-Imidazole (KC-6141), an anti-aggregating compound, onexperimental thrombosis in rats, Thromb Haemost, 1978; 39:74-83): after rat usefulness pentobarbital sodium 30mg/kg ip anesthesia, get internal diameter 0.9mm, be about three sections polyethylene tubes (built-in 6cm long silk thread) of 12cm, connect right common carotid artery and left external jugular vein, Herba Clinopodii in behind the open blood flow 15min, the taking-up silk thread is weighed, this moment, the silk thread weight in wet base deducted former silk thread dry weight, was thrombosed weight in wet base.Gastric infusion group: ig administration Asp, dl-, d-and l-NBP (dosage be respectively 100,200mg/kg) and Asp+l-NBP (dosage respectively is 100mg/kg), promoting the circulation of blood bolt coronary artery bypass grafting as mentioned above behind the 30min.The results are shown in Fig. 1 and 2.
The result shows that the Asp of low dosage (ip) can produce the thrombotic effect of tangible anti-experimental character, 5,10, the Asp of 20mg/kg is respectively 32%, 48% and 52% to thrombotic suppression ratio.L-NBP also can suppress thrombosis in dose dependent ground, and its suppression ratio is respectively 22%, 36% and 69%.The dl-NBP effect obviously is weaker than with the Asp of dosage and l-NBP, and the d-NBP of various dose does not all have obvious effect (see figure 1) to wet weight of thrombus.Behind D1, l-NBP (100, the 200mg/kg) ig40min, the suppression ratio of thrombosis is respectively 26.4%, 32.7% and 28.0%, 36.1%, and the suppression ratio of thrombosis is respectively 35.6% and 54.8% with the Asp of dosage.Each 100mg/kg administering drug combinations thromboembolism preventing effect of l-NBP and Asp is obvious, and the thrombosis suppression ratio is 52.6% (see figure 2).Embodiment 2:dl-, l-and d-NBP are to the influence of extracorporeal platelet aggregation
Rat carotid artery is got blood, prepare according to a conventional method platelet rich plasma (platelet richplasm, PRP) and platelet poor plasma (platelet poor plasm PPP), and transfers with PPP that platelet count is 4.0～6.0 * 10 among the PRP
8Individual/ml, with reference to BornShi method (Herbert JM, Bernat A, Samama M, Maffrand JP, The antiaggregating andantithrombotic activity of ticlopidine is potentiated by aspirinin the rat, Thromb Haemost 1996,76:94-98), get PRP 200 μ l, add dl respectively, (final concentration is 3 for d and l-NBP, 10,30,100 μ mol/l) and Asp (100 μ mol/l or 3-100 μ mol/l), place on the intelligent platelet aggregation instrument, behind 37 ℃ of pre-temperature 5min, add four kinds respectively and lure poly-agent: Coll, ADP, thrombin, AA, its final concentration are respectively 50 μ l/ml, 5 μ mol/l, 1U/ml and 0.5mmol/l.Be determined at the platelet maximum agglutination rate that lures after poly-agent adds 5min.The results are shown in following table 1 and 2.Table 1:NBP and Asp are to the influence (external) of collagen and the effect of the inductive Mus platelet aggregation of ADP
P＜0.05,
*P＜0.01,
* *P＜0.001, compared with the control.Table 2:NBP and Asp are to the influence (external) of the Mus platelet aggregation effect of AA and thrombin induction
P＜0.05,
*P＜0.01,
* *P＜0.001, compared with the control.
| Dosage (μ mol/L) | Collagen-induced (%) | ||||
| Asp | dl-NBP | l-NBP | d-NBP | ||
| Contrast | 78.4±7.1 | ||||
| 3 | 66.3±8.0 * | 60.7±2.8 *** | 74.4±13.6 | ||
| 10 | 58.7±10.2 ** | 50.2±2.0 *** | 72.3±6.0 | ||
| 30 | 49.2±2.2 *** | 35.7±7.0 *** | 57.4±4.7 *** | ||
| 100 | 41.7±7.4 *** | 34.0±5.2 *** | 25.0±10.7 *** | 43.8±8.2 *** | |
| ADP induces (%) | |||||
| Asp | dl-NBP | l-NBP | d-NBP | ||
| Contrast | 72.4±4.7 | ||||
| 3 | 64.1±3.9 ** | 62.4±3.9 ** | 73.1±5.1 | ||
| 10 | 59.1±4.1 *** | 54.6±2.0 *** | 65.5±3.5 * | ||
| 30 | 54.4±7.7 *** | 46.5±3.7 *** | 65.1±8.8 *** | ||
| 100 | 69.1±6.0 | 48.8±2.7 *** | 37.3±2.8 *** | 63.6±7.4 *** | |
| Dosage (μ mol/L) | AA(％) | ||||||
| Asp | dl-NBP | l-NBP | d-NBP | ||||
| Contrast | 78.9±8.6 | ||||||
| 3 | 70.03±13.22 | 69.3±3.5 * | 73.9±5.7 | 66.9±4.2 * | |||
| 10 | 45.52±12.67 *** | 48.2±2.6 ** | 59.2±4.5 *** | 47.1±5.2 *** | |||
| 30 | 37.2±13.02 *** | 33.4±5.2 *** | 33.4±2.7 *** | 41.2±7.4 *** | |||
| 100 | 0±0 *** | 2.6±4.5 *** | 0±0 *** | 4.6±4.1 *** | |||
| Thrombin induction (%) | |||||||
| Asp | dl-NBP | l-NBP | d-NBP | ||||
| Contrast | 72.4±4.7 | ||||||
| 3 | 83.7±5.4 | 85.7±4.5 | 86.4±4.3 | ||||
| 10 | 84.9±7.9 | 82.4±7.5 | 85.9±7.6 | ||||
| 30 | 76.8±16.4 | 72.7±11.8 | 82.2±9.5 | ||||
| 100 | 54.2±7.3 ** | 70.2±7.3 | 70.9±9.9 | 78.6±11.2 | |||
Can obviously find out from the result of table 1 and 2, the dl-of external variable concentrations, d-and l-NBP all have the effect that suppresses Coll, ADP, the inductive platelet aggregation of AA, and be good dose-dependent relationship, the l-NBP effect is the strongest, and when high concentration (100 μ mol/l), can suppress the inductive platelet aggregation of AA fully, and a little less than the d-NBP effect.The platelet aggregation that the dl-of variable concentrations, d-and l-NBP cause thrombin does not have obvious influence.Asp (100 μ ml/l) is all inhibited to the platelet aggregation that Coll, AA, three kinds of derivants of thrombin cause, and the inductive platelet aggregation of ADP is not had obvious influence.Embodiment 3:dl-, l-and d-NBP are to the influence of cAMP content in the platelet
Behind the rabbit anesthesia, the common carotid artery blood sampling with the centrifugal preparation of 120g * 10min PRP, is adjusted platelet count to 2.4 * 10
9Individual/ml, get PRP 0.4ml, Asp, dl-, d-and the l-NBP and Polyethylene Glycol (PEG) contrast that add variable concentrations shown in the according to the form below 3, behind 37 ℃ of pre-temperature 5min, press literature method (Zhang Xiaying, Ren Shuqing, Xiong Jun, a kind of sensitive platelet cyclic adenosine monophosphate (cAMP) algoscopy, Chinese cardiovascular diseases's magazine 1980 8:142-43) is measured cAMP content in the platelet.The results are shown in following table 3.Embodiment 4:dl-, l-and d-BP are to platelet T XA
2The influence of content
Rabbit is got blood, and preparation PRP adjusts platelet count to 1.5 * 10
9Individual/ml, get PRP 180 μ l, according to the form below 3 adds dl-, d-and the l-NBP and the PEG contrast of variable concentrations, behind 37 ℃ of pre-temperature 5min, add AA (final concentration is 0.3mmol/L), behind the 5min, indomethacin cessation reaction with 60 μ g/ml, 200g * 15min is centrifugal, draws supernatant, measures TXB in the sample by the test kit requirement
2Content (Kato K, Sawada S, Toyoda T, Influence ofendothelin on human platelet aggregation and prostacyclingeneration from human vascular endothelial cells in culture, Jpn.Circ.J., 1992,56:422-431).The external effect of table 3:NBP to cAMP and TXA2 concentration in the inductive Mus platelet of AA
Each organizes n=6,
*P＜0.05,
*P＜0.01,
* *P＜0.001.
| Dosage (μ mol/l) | cAMP(mol/10 9Platelet) | ||||
| Dl-NBP | l-NBP | d-NBP | |||
| Contrast | 8.50±3.14 | ||||
| 1.0 | 9.89±2.20 | 11.54±3.59 | 9.20±5.71 | ||
| 10 | 16.52±5.17 ** | 13.92±5.02 * | 8.56±6.67 | ||
| 100 | 16.34±2.93 *** | 19.89±10.44 * | 12.70±7.89 | ||
| TXB 2(μg/10 9Platelet) | |||||
| dl-NBP | l-NBP | d-NBP | |||
| Contrast | 3.88±0.45 | ||||
| 1.0 | 3.60±0.48 | 3.29±0.33 | 3.68±0.35 | ||
| 10 | 3.67±0.28 | 3.57±0.45 | 3.41±0.21 | ||
| 100 | 2.93±0.66 | 2.94±0.41 * | 3.29±0.56 | ||
The result of above table 3 shows, dl, l-NBP (1,10,100 μ mol/l) but cAMP content in the dose dependent ground increased platelets counts, and d-NBP does not have obviously it and acts on.Dl-NBP and d-NBP are to platelet T XA simultaneously
2Content does not have obvious influence, l-NBP only when high concentration to TXA
2Content raises more weak inhibitory action.The influence that embodiment 5:dl-, l-and d-NBP discharge platelet 5-hydroxy tryptamine (5-HT)
Preparation rabbit PRP, adding final concentration respectively is 0.1,1.0,10, the dl-of 100 μ mol/l, d-and l-NBP, Asp 100 μ ml/l and PEG contrast, behind 37 ℃ of pre-temperature 5min, assemble back 5min at collagen-induced platelet, cessation reaction, press Curzon method (CurzonG, Green AR., Rapid method for the determination of 5-hydroxytryptamine and 5-hydroxyindoleacetic acid in smallregions of rat brain, Br J Pharmacol, 1970,39:653-56) prepare sample, and measure the 5-HT release rate with spectrofluorophotometer (exciting light 365nm, emission light 480nm).The results are shown in Figure 3.
As can be seen from Figure 3, but the remarkable inhibition platelet 5-HT release of l-NBP dose dependent, and dl, d-NBP then act on not obvious.Asp (100 μ mol/m1) discharges 5-HT does not also have the obvious suppression effect.Embodiment 6:l-NBP is to the influence of extracorporeal platelet aggregation
The health volunteer, the male, 28-30 year, at 8 o'clock in the morning oral l-NBP 200mg/ people.After taking medicine, behind 30min, 1h, 2h, 3h and 4h, get blood, 3ml/ time from ulnar vein.Prepare platelet rich plasma (PRP) and platelet poor plasma (PPP) according to a conventional method, and transfer with PPP that platelet count is 4.0～6.0 * 10 among the PRP
8Individual/ml, with reference to BornShi method (the same), get PRP 200 μ l, add dl, d and l-NBP (final concentration is 3,10,30,100 μ mol/l) and Asp respectively, place on the intelligent platelet aggregation instrument, behind 37 ℃ of pre-temperature 5min, adding lures poly-agent: ADP, AA, its final concentration to be respectively 5 μ mol/l and 0.5mmol/l.Be determined at the platelet maximum agglutination rate that lures after poly-agent adds 5min.The result is shown in Figure 4 and 5.
By Figure 4 and 5 as can be seen, before taking medicine, ADP and AA lure imported platelet aggregation rate to be respectively 55.2 ± 6.3% and 55.8 ± 8.0%.Took medicine back 0.5 hour, the platelet aggregation rate calibration that ADP causes does not often have significant change, but between 1 hour to 3 hours, platelet aggregation rate continues to descend, and is respectively 37.3 ± 7.5%, 26 ± 15.6% and 24.7 ± 2.1%.Took medicine back 4 hours, platelet aggregation rate recovers to some extent, is 39.7 ± 5.9%.Inductive platelet aggregation effect slightly is later than ADP to l-NBP to AA, and platelet aggregation rate is respectively 52.8 ± 9.3%, 47.8 ± 9.3%, 28.7 ± 14.5%, 29.3 ± 5.7% and 43.3 ± 10.7% behind take medicine back 0.5h, 1h, 2h, 3h and the 4h.And the experimenter take medicine the back readme do not have sense of discomfort.
Claims (4)
1, the application of butylphthalide in the preparation anti-thrombosis drug.
2, application as claimed in claim 1, wherein, described butylphthalide is left-handed butylphthalide.
3, the application of butylphthalide in the preparation medicament for resisting platelet aggregation.
4, application as claimed in claim 3, wherein, described butylphthalide is left-handed butylphthalide.
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| US7678776B2 (en) | 2002-08-21 | 2010-03-16 | Shijiazhuang Pharma. Group Zhongqi Pharmaceutical Technology (Shijiazhuang) Co., Ltd. | Inclusion complexes of butylphthalide with cyclodextrin or its derivatives, a process for their preparation and the use thereof |
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| CN1605336A (en) | 2003-10-10 | 2005-04-13 | 中国医学科学院药物研究所 | Application of L-butylphthalide in the process for preparing cerebral infarction preventing and treating medicine |
| CN1257711C (en) * | 2003-12-05 | 2006-05-31 | 石药集团中奇制药技术（石家庄）有限公司 | Butyl benzene phthalein soft capsule and its preparation method |
| CN1647796A (en) | 2004-01-20 | 2005-08-03 | 中奇制药技术（石家庄）有限公司 | Use of Levo-butylphthalide in preparing medicine for preventing and treating cerebral ischemia diseases |
| CN101627992B (en) * | 2004-01-20 | 2012-09-12 | 石药集团中奇制药技术（石家庄）有限公司 | Application of levo butyl phthalide in preparing brain stroke medicament |
| CN1331469C (en) * | 2004-04-05 | 2007-08-15 | 王弢 | Use of dipolymer of phthalide |
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| CN100361656C (en) | 2004-08-27 | 2008-01-16 | 石药集团中奇制药技术（石家庄）有限公司 | Butylbenzene phthalein self emulsifying releasing medicine system, preparation method and application |
| CN100367951C (en) | 2005-12-16 | 2008-02-13 | 石药集团恩必普药业有限公司 | Butyl benzene phthalein vein emulsion and its application |
| CN101289438B (en) * | 2007-04-16 | 2012-04-18 | 山东绿叶天然药物研究开发有限公司 | 3-(3'-hydroxyl)-butyl phthalide ester, and preparation thereof and uses |
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| CN103655555B (en) | 2012-09-14 | 2018-08-28 | 中山大学 | Application of the 3- normal-butyls -1-isoindolinone in the drug for preparing prevention and treatment cerebral infarction |
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|---|
| 中国工理学报18(6) 1997.1.1 种兆钟 丁基苯酞对大鼠局灶性脑缺血和重罐后脑内TXB和6KETO-PGFLA含量的影响 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7678776B2 (en) | 2002-08-21 | 2010-03-16 | Shijiazhuang Pharma. Group Zhongqi Pharmaceutical Technology (Shijiazhuang) Co., Ltd. | Inclusion complexes of butylphthalide with cyclodextrin or its derivatives, a process for their preparation and the use thereof |
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| CN1257706A (en) | 2000-06-28 |
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