CN108693308A - A kind of method of Fast Evaluation strawberry cultivars petal falling speed - Google Patents
A kind of method of Fast Evaluation strawberry cultivars petal falling speed Download PDFInfo
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- CN108693308A CN108693308A CN201810327930.6A CN201810327930A CN108693308A CN 108693308 A CN108693308 A CN 108693308A CN 201810327930 A CN201810327930 A CN 201810327930A CN 108693308 A CN108693308 A CN 108693308A
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Abstract
The invention discloses a kind of methods of Fast Evaluation strawberry cultivars petal falling speed, including flower standard for manual sampling, cultured in vitro environment, falling speed evaluation criterion;The present invention, then by manually rejecting inhomogenous flower, ensures the stability of evaluation result, passes through the environmental condition of fixed cultured in vitro, the repeatability of guarantee test result by collecting the preferable flower of consistency in flower sample phase.The present invention overcomes existing strawberry petal falling speed evaluation whole process to carry out in vinyl house, it is influenced by Changes in weather during evaluation, the environmental conditions such as temperature, humidity in greenhouse are unstable in evaluation procedure, strawberry petal falling speed is also easily influenced by changes in environmental conditions, lead to the defect that the result repeatability that different time measures is bad, strawberry flower is removed and carries out cultured in vitro under the conditions of being placed in stable environment, different time measure result with strawberry Performance of cultivar is consistent in actual production and test repeatability is more preferable compared with prior art.
Description
Technical field
The invention belongs to agricultural plant germplasm resource evaluation method and technology fields, and in particular to a kind of Fast Evaluation strawberry product
The method of kind petal falling speed.
Background technology
Strawberry is that China is generally cultivated, and has both the fruit of high nutritive value and economic value.Gray mold is strawberry growth period
With the Major Diseases of storage phase, generally occur in the whole world.Cause the cause of disease Botrytis cinerea (Botrytis of grey mould fruit rot of strawberry
Cinerea it is) a kind of necrotrophic fungus of non-specialization, latent or dormant state is kept in health tissues, is infected
Tissue usually not symptom, when tissue die, pathogen extends rapidly and generates conidium.Botrytis cinerea needs first
It is colonized on the plant tissue of aging or death, it is thin to generate hydrolase, cell wall degrading enzyme etc. using the nutrition on aging tissues
Cellular toxicity object disintegrates the defence line of health plant tissue, is then diffused into health tissues from the aging tissues previously colonized.Strawberry
Valve rapid aging after the completion of pollination, the petal of aging are the important intermediaries that Botrytis cinerea infects fruit in development.
Petal falling speed is the important indicator for evaluating strawberry cultivars gray mold resistance level, and the prior art passes through in field
Directly petal falling speed is evaluated.But strawberry petal falling speed is easy the shadow by environmental conditions such as temperature, humidity
It rings, existing method evaluation result is unstable, and evaluation procedure is time-consuming.
Invention content
The present invention is for the defect that overcomes existing evaluation method unstable result, evaluation procedure time-consuming, it is therefore an objective to be to carry
For a kind of method of Fast Evaluation strawberry cultivars petal falling speed, carried for the crossbreeding or molecular mark of strawberry
For supporting.
The present invention technical concept be:A kind of method of Fast Evaluation strawberry petal falling speed of the present invention, including flower
Standard for manual sampling, cultured in vitro environment, falling speed evaluation criterion.The present invention is preferable by collecting consistency in flower sample phase
Flower ensure the stability of evaluation result then by manually rejecting inhomogenous flower.Pass through the ring of fixed cultured in vitro
Border condition, the repeatability of guarantee test result.
To solve the above problems, the technical solution adopted by the present invention is:
A kind of method of Fast Evaluation strawberry petal falling speed, includes the following steps:
(1) when strawberry flower just starts to budding, each variety selection 30 just starts the flower buddingged, and flower is connected
Anthocaulus is taken from plant together;
(2) flower is inserted in the water agar of solidification, is placed in cultured in vitro under 15-30 DEG C of constant temperature and illumination condition;
(3) after cultured in vitro 1-24 hours, the flower that petal is not yet fully deployed is rejected, it is ensured that each kind have 20 with
The flower that upper petal is fully deployed records every colored petal quantity, calculates petal sum;
(4) cultured in vitro is gently shaken after 2-4 days, and the petal completely disengaged with sepal is made to fall;
(5) remaining petal number on flower is recorded, petal expulsion rate is calculated;
Petal expulsion rate calculation formula is:
(6) petal falling speed is classified:The 4th day petal expulsion rate is de- to its petal after being buddingged according to different strawberry cultivars
Terminal-velocity degree is classified, and criteria for classification is as follows:
Preferably, the mass content of agar is 0.5-5% in the water agar.
Compared with prior art, technique effect of the invention is:
(1) existing strawberry petal falling speed evaluation whole process is carried out in vinyl house, by Changes in weather during evaluation
It influences, the environmental conditions such as temperature, humidity in greenhouse are unstable in evaluation procedure, and strawberry petal falling speed is also easily by ring
The influence of border condition variation, causes the result repeatability that different time measures bad.Strawberry flower is removed in the present invention and is placed in
Cultured in vitro is carried out under the conditions of stable environment, the result that different time measures is consistent and tries with strawberry Performance of cultivar in actual production
It is more preferable compared with prior art to test repeatability, is more easy to be applied by not commensurate;
(2) it need to one by one be hung on flower when flower just starts to budding in existing strawberry petal falling speed evaluation procedure
Board is marked with the date of buddingging to flower, also needs to find listed flower in numerous strawberry flowers in greenhouse after 7 days
It is evaluated, whole process is complicated, not easy to operate.The flower buddingged just is directly adopted lower concentration by the method for the present invention since field
Cultured in vitro is observed, easy to operate;
(3) existing strawberry petal falling speed evaluation procedure needs 7 days altogether from start to end, is entirely evaluated in the present invention
4 days are needed in the process, and evaluation is more quick.
Specific implementation mode
Technical scheme of the present invention is further elaborated with reference to embodiment:
Embodiment 1
A kind of method of Fast Evaluation strawberry cultivars petal falling speed, step are:
(1) when strawberry flower just starts to budding, each variety selection 30 just starts the flower buddingged, and flower is connected
Anthocaulus is taken from plant together;
(2) flower is inserted in the water agar (agar powder ratio is 1.5%) of solidification, is placed in 25 DEG C of constant temperature and illumination condition
Lower cultured in vitro;
(3) cultured in vitro rejected the flower that petal is not yet fully deployed after 12 hours, it is ensured that each kind has 20 or more
The flower that petal is fully deployed records every colored petal quantity, calculates petal sum;
(4) cultured in vitro is gently shaken after 4 days, and the petal completely disengaged with sepal is made to fall;
(5) the remaining petal number on flower is recorded, petal expulsion rate is calculated;
Petal expulsion rate calculation formula is:
(6) petal falling speed is classified:The 4th day petal expulsion rate is de- to its petal after being buddingged according to different strawberry cultivars
Terminal-velocity degree is classified, and criteria for classification is as follows:
Strawberry cultivars in the step (1) are ' sweet tea Cha Li ', ' brilliant jade ', ' brilliant precious jade ', ' Feng Xiang ' and ' beauty ', when implementation
Between be in January, 2018.
Embodiment 2:
A kind of method of Fast Evaluation strawberry cultivars petal falling speed, step are:
(1) when strawberry flower just starts to budding, each variety selection 30 just starts the flower buddingged, and flower is connected
Anthocaulus is taken from plant together;
(2) flower is inserted in the water agar (agar powder ratio is 1.5%) of solidification, is placed in 25 DEG C of constant temperature and illumination condition
Lower cultured in vitro;
(3) cultured in vitro rejected the flower that petal is not yet fully deployed after 12 hours, it is ensured that each kind has 20 or more
The flower that petal is fully deployed records every colored petal quantity, calculates petal sum;
(4) cultured in vitro is gently shaken after 4 days, and the petal completely disengaged with sepal is made to fall;
(5) the remaining petal number on flower is recorded, petal expulsion rate is calculated;
Petal expulsion rate calculation formula is:
(6) petal falling speed is classified:The 4th day petal expulsion rate is de- to its petal after being buddingged according to different strawberry cultivars
Terminal-velocity degree is classified, and criteria for classification is as follows:
Strawberry cultivars in the step (1) are ' sweet tea Cha Li ', ' brilliant jade ', ' brilliant precious jade ', ' Feng Xiang ' and ' beauty ', when implementation
Between be 2 months 2018.
Embodiment 3:
A kind of method of Fast Evaluation strawberry cultivars petal falling speed, step are:
(1) when strawberry flower just starts to budding, each variety selection 30 just starts the flower buddingged, and flower is connected
Anthocaulus is taken from plant together;
(2) flower is inserted in the water agar (agar powder ratio is 1.5%) of solidification, is placed in 25 DEG C of constant temperature and illumination condition
Lower cultured in vitro;
(3) cultured in vitro rejected the flower that petal is not yet fully deployed after 12 hours, it is ensured that each kind has 20 or more
The flower that petal is fully deployed records every colored petal quantity, calculates petal sum;
(4) cultured in vitro is gently shaken after 4 days, and the petal completely disengaged with sepal is made to fall;
(5) the remaining petal number on flower is recorded, petal expulsion rate is calculated;
Petal expulsion rate calculation formula is:
(6) petal falling speed is classified:The 4th day petal expulsion rate is de- to its petal after being buddingged according to different strawberry cultivars
Terminal-velocity degree is classified, and criteria for classification is as follows:
Strawberry cultivars in the step (1) are ' sweet tea Cha Li ', ' brilliant jade ', ' brilliant precious jade ', ' Feng Xiang ' and ' beauty ', when implementation
Between be in March, 2018.
Comparative example 1:
A method of evaluation strawberry cultivars petal falling speed, step are:
(1) when flower just starts to budding, each variety selection 20 just starts the flower buddingged, and hangs and hangs on anthocaulus
Board simultaneously records the time buddingged;
(2) 1-2 days after buddingging, the every petal quantity taken is recorded after petal is fully deployed;
(3) the remaining petal number on flower is recorded after buddingging 7 days, calculates petal expulsion rate.Petal expulsion rate calculation formula
For:
Strawberry cultivars in the step 1 are ' sweet tea Cha Li ', ' brilliant jade ', ' brilliant precious jade ', ' Feng Xiang ' and ' beauty ', implement the time
For in January, 2014.
Comparative example 2:
A method of evaluation strawberry cultivars petal falling speed, step are:
(1) when flower just starts to budding, each variety selection 20 just starts the flower buddingged, and hangs and hangs on anthocaulus
Board simultaneously records the time buddingged;
(2) 1-2 days after buddingging, the every petal quantity taken is recorded after petal is fully deployed;
(3) the remaining petal number on flower is recorded after buddingging 7 days, calculates petal expulsion rate.Petal expulsion rate calculation formula
For:
Strawberry cultivars in the step 1 are ' sweet tea Cha Li ', ' brilliant jade ', ' brilliant precious jade ', ' Feng Xiang ' and ' beauty ', implement the time
For in March, 2014.
Comparative example 3:
A method of evaluation strawberry cultivars petal falling speed, step are:
(1) when flower just starts to budding, each variety selection 20 just starts the flower buddingged, and hangs and hangs on anthocaulus
Board simultaneously records the time buddingged;
(2) 1-2 days after buddingging, the every petal quantity taken is recorded after petal is fully deployed;
(3) the remaining petal number on flower is recorded after buddingging 7 days, calculates petal expulsion rate.Petal expulsion rate calculation formula
For:
Strawberry cultivars in the step 1 are ' sweet tea Cha Li ', ' brilliant jade ', ' brilliant precious jade ', ' Feng Xiang ' and ' beauty ', implement the time
For in March, 2015.
Table 1:Petal expulsion rate and falling speed classification at embodiment and comparative example difference strawberry product
Table 1 the result shows that:Carry out from field strawberrying flower in different time in embodiment and tests, different strawberry product
Kind petal expulsion rate test result stability and reproducible.
It is slow by low temperature effect pollen loose powder since environment temperature is low during experiment in comparative example 1, it is different after buddingging 7 days
The petal falling speed of strawberry cultivars is 0.0%.Although petal falls off between different strawberry cultivars in comparative example 2 and comparative example 3
The entirety sequence of rate is basicly stable but less reproducible, and absolute figure difference is big.
Finally it should be noted that:The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
With technical scheme described in the above embodiments is modified or equivalent replacement of some of the technical features.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in the present invention's
Within protection domain.
Claims (2)
1. a kind of method of Fast Evaluation strawberry petal falling speed, which is characterized in that include the following steps:
(1) when strawberry flower just starts to budding, each variety selection 30 just starts the flower buddingged, and flower is connected anthocaulus
It is taken from plant together;
(2) flower is inserted in the water agar of solidification, is placed in cultured in vitro under 15-30 DEG C of constant temperature and illumination condition;
(3) after cultured in vitro 1-24 hours, the flower that petal is not yet fully deployed is rejected, it is ensured that each kind there are 20 or more flowers
The flower that valve is fully deployed records every colored petal quantity, calculates petal sum;
(4) cultured in vitro is gently shaken after 2-4 days, and the petal completely disengaged with sepal is made to fall;
(5) remaining petal number on flower is recorded, petal expulsion rate is calculated;
Petal expulsion rate calculation formula is:
(6) petal falling speed is classified:The 4th day petal expulsion rate falls off speed to its petal after being buddingged according to different strawberry cultivars
Degree is classified, and criteria for classification is as follows:
2. according to the method described in claim 1, it is characterized in that, the mass content of agar is about 0.5- in the water agar
5.0%.
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| CN201810327930.6A CN108693308A (en) | 2018-04-12 | 2018-04-12 | A kind of method of Fast Evaluation strawberry cultivars petal falling speed |
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| CN201810327930.6A CN108693308A (en) | 2018-04-12 | 2018-04-12 | A kind of method of Fast Evaluation strawberry cultivars petal falling speed |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113186323A (en) * | 2020-12-17 | 2021-07-30 | 湖北省农业科学院经济作物研究所 | KASP molecular marker related to strawberry petal shedding speed character and application thereof |
Citations (4)
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| CN103270893A (en) * | 2013-05-31 | 2013-09-04 | 湖北省农业科学院经济作物研究所 | Method for appraising resistance of strawberries to gray mold |
| CN103535279A (en) * | 2013-10-21 | 2014-01-29 | 中国林业科学研究院林业研究所 | Rooting method for un-rooted tissue culture seedlings of salix matsudana var.tortuosa |
| CN103571789A (en) * | 2013-11-13 | 2014-02-12 | 江苏省农业科学院 | Jasmine pollen in-vitro germination liquid culture medium and method for measuring activity of jasmine pollen |
| CN103837534A (en) * | 2014-02-25 | 2014-06-04 | 浙江大学 | Method for testing pollen viability of white peony root |
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2018
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103270893A (en) * | 2013-05-31 | 2013-09-04 | 湖北省农业科学院经济作物研究所 | Method for appraising resistance of strawberries to gray mold |
| CN103535279A (en) * | 2013-10-21 | 2014-01-29 | 中国林业科学研究院林业研究所 | Rooting method for un-rooted tissue culture seedlings of salix matsudana var.tortuosa |
| CN103571789A (en) * | 2013-11-13 | 2014-02-12 | 江苏省农业科学院 | Jasmine pollen in-vitro germination liquid culture medium and method for measuring activity of jasmine pollen |
| CN103837534A (en) * | 2014-02-25 | 2014-06-04 | 浙江大学 | Method for testing pollen viability of white peony root |
Non-Patent Citations (1)
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113186323A (en) * | 2020-12-17 | 2021-07-30 | 湖北省农业科学院经济作物研究所 | KASP molecular marker related to strawberry petal shedding speed character and application thereof |
| CN113186323B (en) * | 2020-12-17 | 2024-02-09 | 湖北省农业科学院经济作物研究所 | KASP molecular marker related to falling speed of strawberry petals and application thereof |
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Application publication date: 20181023 |
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