CN108693180A - A kind of chilled poultry storage and transportation freshness safety warning card and preparation and use - Google Patents
A kind of chilled poultry storage and transportation freshness safety warning card and preparation and use Download PDFInfo
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- CN108693180A CN108693180A CN201810696787.8A CN201810696787A CN108693180A CN 108693180 A CN108693180 A CN 108693180A CN 201810696787 A CN201810696787 A CN 201810696787A CN 108693180 A CN108693180 A CN 108693180A
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- storage
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- chilled
- chilled poultry
- card
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- 244000144977 poultry Species 0.000 title claims abstract description 82
- 238000003860 storage Methods 0.000 title claims abstract description 62
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 241000894006 Bacteria Species 0.000 claims abstract description 88
- 239000007788 liquid Substances 0.000 claims abstract description 36
- 241000287828 Gallus gallus Species 0.000 claims abstract description 33
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 33
- 239000000284 extract Substances 0.000 claims abstract description 29
- 238000004806 packaging method and process Methods 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 18
- 239000011780 sodium chloride Substances 0.000 claims abstract description 18
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims abstract description 10
- 238000007789 sealing Methods 0.000 claims abstract description 9
- 239000000126 substance Substances 0.000 claims description 11
- 230000008569 process Effects 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 241000532370 Atla Species 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 6
- 239000012137 tryptone Substances 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 3
- 235000015278 beef Nutrition 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 3
- 210000000988 bone and bone Anatomy 0.000 claims description 3
- 239000000084 colloidal system Substances 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 230000008595 infiltration Effects 0.000 claims description 3
- 238000001764 infiltration Methods 0.000 claims description 3
- 238000003801 milling Methods 0.000 claims description 3
- 238000001694 spray drying Methods 0.000 claims description 3
- 238000005538 encapsulation Methods 0.000 claims description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 2
- 239000011707 mineral Substances 0.000 claims description 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- 230000011218 segmentation Effects 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 238000010521 absorption reaction Methods 0.000 claims 1
- 235000021393 food security Nutrition 0.000 abstract description 2
- 238000012544 monitoring process Methods 0.000 abstract description 2
- 235000013372 meat Nutrition 0.000 description 13
- 238000012856 packing Methods 0.000 description 9
- 244000144972 livestock Species 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000006701 autoxidation reaction Methods 0.000 description 1
- 206010064097 avian influenza Diseases 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 108010046845 tryptones Proteins 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
The invention belongs to agricultural product and food security on-line monitoring technique field, preparation and the application method of a kind of chilled poultry storage and transportation freshness safety warning card are disclosed.Chilled poultry storage and transportation freshness safety warning card of the present invention is sealed in container, including has adsorbed the bacterium solution packet of the filter paper and sealing of colour developing enrichment liquid.The enrichment liquid that develops the color includes Chicken extract, sodium chloride, 2,3, 5-Triphenyltertrazoliumchloride;Bacterium solution packet is 102-103Cfu/mL pseudomonas aeruginosas.The chilled poultry storage and transportation freshness safety warning is stuck in before chilled poultry enters finished bin, pinches brokenly the closed poly-bag containing bacterium warned in card packaging bag, and card of warning is sufficiently humidified so as to, and keeps sealing state, be placed on outside the packaging bag of chilled poultry, storage and transportation together.When consumer buys chilled poultry, the color for card of warning is observed, judges the freshness of poultry.
Description
Technical field
The invention belongs to agricultural product and food security on-line monitoring technique field, and in particular, to a kind of chilled poultry storage
Transport preparation and the application method of freshness safety warning card.
Background technology
It takes place frequently with the propulsion of urbanization process with bird flu epidemic situation, has driven the poultry market of farm produce now to be killed and now sell pattern
The transformation of pattern is butchered to concentration, live-bird sale turns to fresh or freezing sale.All carrying forward vigorously " poultry concentration both at home and abroad
Butcher, the listing of fresh poultry ", after butchering fowl, body surface residual microbial, in the growth of storage and transport process, to accelerate poultry
It is putrid and deteriorated.Since consumer can not be by visually judging freshness and the safety of chilled poultry, to affect ice
The market acceptance level of fresh poultry.In recent years, various quick, lossless chilled poultry safety detection technologies become research heat
Point, such as resistance detection method, spectrum detection method.But these methods are required for large-scale instrument and special messenger to operate, and are not suitable for daily life
Production, storage and transportation, sale and consumer intuitively judge when purchasing.
Invention content
It is an object of the present invention to provide a kind of chilled poultry storage and transportation freshness safety warning card, easy to operate, judgement is intuitively
It is illustrated, is easy to use, is suitble to chilled poultry cold chain storage and transportation consigner new to product to the supervision of cold chain transportation and terminal consumer
Freshness, safety judgement.
In order to achieve the above object, the technical solution adopted by the present invention is:A kind of chilled poultry storage and transportation freshness safety warning
Card, card of warning are sealed in container, including have adsorbed the bacterium solution packet of the filter paper and sealing of colour developing enrichment liquid.
Further, the filter paper uses 25cm2 middling speed qualitative filter papers.
Further, the colour developing enrichment liquid be Chicken extract, sodium chloride, 2,3, 5-Triphenyltertrazoliumchloride press than
Example is dissolved in the water to obtain mixed liquor.
Further, the bacterium solution packet is the pseudomonas aeruginosa of quantitative filling, bacteria concentration 102-103cfu/mL.
A kind of preparation method of chilled poultry storage and transportation freshness safety warning card described in one, includes the following steps:
(A)Bacterium solution packs standby, and pseudomonas aeruginosa is activated with beef extract tryptone culture medium to 109 cfu/mL, 3000r/
Min centrifuges 20min, obtains thalline, sterile saline is added to be diluted to 102-103cfu/mL, quantitative filling to sterile bag, 10-
15 DEG C of preservations.
(B)Prepared by the enrichment liquid that develops the color, Chicken extract, sodium chloride, 2,3, 5-Triphenyltertrazoliumchloride is molten in proportion
Colour developing enrichment liquid is obtained in Xie Yushui;
(C), impregnate, filter paper be soaked in 3 ~ 10s in the colour developing enrichment liquid, draining, 45-50 DEG C of drying, drying preservation;
(D)The filter paper of above-mentioned gained enrichment liquid containing colour developing and the filling bacterium solution to sterile bag are packaged into same closed appearance by encapsulation
In device, it is ensured that after sealing, remaining filtrated air is grown for indicator bacteria in bag, and box height ensures bacterium solution packet in the complete of storage process
Whole property.
Preferably, in Chicken extract described in the Chicken extract, small peptide content >=50% of molecular weight≤3000, always
Protein >=90%, mineral salt >=1%.
Preferably, the Chicken extract obtains as follows:Take the chicken carcasses cleaning after segmentation;Boiling water 10min is killed
Bacterium;It is cooled to 55-60 DEG C, enzymolysis 5-8 h;Remaining substance is through milling treatment of colloid after removing bone;It is concentrated in vacuo to 0.5g/mL;
Spray drying obtains Chicken extract pulvis.
Preferably, the enzymolysis uses enzyme activity for the trypsase of 200kU/g, in solid-liquid ratio 1:3, enzyme concentration 2.8-
It is carried out under conditions of 6.7 kU/g, enzymolysis time 5-8 h.
Preferably, when cleaning, ultrasonic wave 440w handles 10min after infiltration.
Preferably, Chicken extract, sodium chloride, 2,3, 5-Triphenyltertrazoliumchloride are in proportion(700-1000):
(450-550):(1-2).
A kind of use of chilled poultry storage and transportation freshness safety warning card is pinched brokenly closed before chilled poultry enters finished bin
The bacterium solution packet warned in card packaging bag, card of warning are sufficiently humidified so as to, and are continued to keep sealing state, are placed on the packaging bag of chilled poultry
Outside, storage and transportation together;Matching used control colour atla is directly printed in packaging;The color of comparison control colour atla, it can be determined that should
Whether whether chilled poultry qualified in storage and transport process.
Beneficial effects of the present invention:
1, chilled poultry of the invention is warned card, is built upon to poultry surface, lumen microbial type and growth characteristics research
On the basis of, pointedly select content at most, in chilled poultry surface well-grown, the cause corruption bacterium for causing poultry rotten ---
Pseudomonas aeruginosa.Within the chilled poultry shelf-life, if reserve temperature reaches 4 DEG C hereinafter, object bacteria can self-dissolving death;10℃
Left and right growth is extremely slow;The fast-growth of the beginning more than 25 DEG C.Therefore, the indicator bacteria can indicate chilled chicken storage and transportation, sell
The temperature of journey controls.
2, the indicator selected by the present invention is 2,3, 5-Triphenyltertrazoliumchloride, is in the instruction of growth and breeding state
Bacterium, bacterial dehydrogenase can make colourless TTC, receive hydrogen as red, red to make bacterium colony present, maximum feature is just
It is in the case of having oxygen, will not be by autoxidation, the accuracy for the card that influences to warn.
3, the effect for the Chicken extract culture indicator bacteria selected in present invention increasing microbial inoculum is better than conventional tryptone etc., together
The indicator bacteria viable bacteria recall rate of sample concentration is 10-15 times high;There is protection to make indicator bacteria in the case where Chicken extract is for low temperature condition
With being handled 7 days at 4 DEG C, pseudomonas aeruginosa survival rate reaches 30% or more;Molecular weight is small, good water solubility, can be by card of warning
Carrier adsorbs completely, and enriching effect is notable;It has been investigated that Chicken extract has pseudomonas aeruginosa promotes growth well
Effect, growth rate of the purpose bacterium in nutrient solution are consistent with chilled poultry body surface.
4, indicator bacteria is packaged in bacterium card is increased in a confined space by the present invention simultaneously, is placed in outside chilled chicken packaging bag
In crisper, it ensure that safety warning card will not pollute chilled poultry.
5, the reaction of color and initial microbial population, temperature, storage time have relationship, if there is bacterium growth, bacterium number is more than
104Cfu/mL, card color of warning will switch to red by colourless, and to warn limit value, color is deeper, illustrates that bacterium number is more;This shows
Alert card meets safe and nontoxic, non-harmful theory, have it is easy to operate, it is easy to use, be easy to the advantage that observation judges, be suitble to
Judgement of the chilled poultry cold chain storage and transportation consigner to the supervision and terminal consumer of cold chain transportation to product freshness, safety.
Specific implementation mode
In order to make those skilled in the art more fully understand technical scheme of the present invention, the present invention is retouched in detail below
It states, the description of this part is only exemplary and explanatory, should not there is any restriction effect to protection scope of the present invention.
The preparation method of Chicken extract described in embodiment is:Chicken carcasses clean;Ultrasonic wave 440w processing after infiltration
10min;Boiling water 10min sterilizations;It is cooled to 55-60 DEG C, 2.8-6.7 kU/g pancreatin, enzymolysis time 5-8 h is added;Remove bone
Remaining substance is through milling treatment of colloid afterwards;It is concentrated in vacuo to 0.5g/mL;Spray drying obtains Chicken extract pulvis.
Filter paper is warned the preparation method of card:The Chicken extract, sodium chloride, 2,3, 5-Triphenyltertrazoliumchloride are pressed
Ratio is dissolved in the water to obtain colour developing enrichment liquid, the qualitative thick filter paper of 25cm2 middling speeds is soaked in 3 in the colour developing enrichment liquid ~
10s, draining, 45-50 DEG C of drying, drying preservation.
The preparation method of bacterium solution packet is in chilled poultry storage and transportation freshness safety warning card:By pseudomonas aeruginosa beef
Cream tryptone culture medium is activated to 109 Cfu/mL, 3000r/m centrifuge 20min, obtain thalline, sterile saline is added to dilute
To 109 Cfu/mL, quantitative filling 1mL to sterile bag, 10-15 DEG C of preservation.
The manner of packing of above-mentioned chilled poultry storage and transportation freshness safety warning card, in desinfection chamber, by above-mentioned gained containing aobvious
Color enrichment liquid filter paper and bacterium solution are packaged into same closed plastic box, and plastic casing inside casing area is 25cm2, it is highly 0.5cm;Really
Remaining filtrated air is grown for indicator bacteria in confidential letter back pkt., integrality of the box height guarantee bacterium solution packet in storage process.
In use, before chilled poultry enters finished bin, the closed bacterium solution packet warned in card packaging bag is pinched brokenly, card of warning is abundant
Moistening continues to keep sealing state, be placed on outside the packaging bag of chilled poultry, together storage and transportation;Matching used control colour atla is direct
It is printed on the packaging;Comparison control colour atla color, it can be determined that the chilled poultry whether in storage and transport process whether preservation temperature
High or overlong time is spent, there is security risk.
Embodiment 1
The present embodiment provides a kind of chilled poultry storage and transportation freshness safety warning card, indicator bacteria develops the color enrichment liquid by following quality hundred
The each group of ratio is divided to be grouped as:7.0 mg Chicken extracts, 4.5 mg sodium chloride, 0.01 mg TTC.According to the above ratio by three kinds
Substance is compounded, and is mixed well to get indicator bacteria colour developing enrichment liquid, is adsorbed in 25cm2Filter paper.
A concentration of the 10 of 1mL2Cfu/mL P. aeruginosas bacterium solution is by closed filling in small plastic bag.With warn card together
It is packaged in closed polybag.
In use, racking the bacterium bag in packaging, so that bacterium solution is evenly distributed on card of warning, be placed in the outer packing of chilled poultry
.
Within the shelf-life of chilled poultry(0-4 DEG C, 7d), when chilled poultry storage and transportation temperature reaches"Livestock meat cold chain transportation
Administrative skill specification"(GB/T 28640)Standard, i.e., chilled meat central temperature are no more than 0-4 DEG C, and storage and transportation environment is not higher than 7 DEG C,
Indicator bacteria cannot grow, and red is not presented in card of warning.
Embodiment 2
The present embodiment provides a kind of chilled poultry storage and transportation freshness safety warning cards, and indicator bacteria enrichment liquid is by following mass percent
Each group be grouped as:9.0 mg Chicken extracts, 5.0 mg sodium chloride, 0.01 mg TTC.According to the above ratio by three kinds of substances
It is compounded, mixes well to get the indicator bacteria enrichment liquid, be adsorbed in 25cm2Filter paper.Inventor presses above-mentioned three kinds of substances
Certain proportion compounds, and the speed of growth of pseudomonas aeruginosa wherein can be allowed consistent in the poultry surface speed of growth, be in
Colour response can accurately react the cause corruption microbiology turbidity situation of chilled poultry body surface, illustrate the new of chilled poultry indirectly
Freshness and edible safety.
A concentration of the 10 of 1mL2Cfu/mL P. aeruginosas bacterium solution is by closed filling in small plastic bag.With warn card together
It is packaged in closed polybag or plastic casing.
In use, racking the bacterium bag in packaging, so that bacterium solution is evenly distributed on card of warning, be placed in the outer packing of chilled poultry
.
Within the shelf-life of chilled poultry(0-4 DEG C, 7d), when chilled poultry storage and transportation temperature reaches"Livestock meat cold chain transportation
Administrative skill specification"(GB/T 28640)Standard, i.e., chilled meat central temperature are no more than 0-4 DEG C, and storage and transportation environment is not higher than 7 DEG C,
Indicator bacteria cannot grow, and red is not presented in card of warning.
Embodiment 3
The present embodiment provides a kind of chilled poultry storage and transportation freshness safety warning cards, and indicator bacteria enrichment liquid is by following mass percent
Each group be grouped as:10. 0 mg Chicken extracts, 5.5 mg sodium chloride, 0.02 mg TTC.According to the above ratio by three kinds
Substance is compounded, and is mixed well to get the indicator bacteria enrichment liquid, is adsorbed in 25cm2Filter paper.
A concentration of the 10 of 1mL3Cfu/mL P. aeruginosas bacterium solution is by closed filling in small plastic bag.With warn card together
It is packaged in closed polybag.
In use, racking the bacterium bag in packaging, so that bacterium solution is evenly distributed on card of warning, be placed in the outer packing of chilled poultry
.
Within the shelf-life of chilled poultry(0-4 DEG C, 7d), when chilled poultry storage and transportation temperature reaches"Livestock meat cold chain transportation
Administrative skill specification"(GB/T 28640)Standard, i.e., chilled meat central temperature are no more than 0-4 DEG C, and storage and transportation environment is not higher than 7 DEG C,
Indicator bacteria cannot grow, and red is not presented in card of warning.
Embodiment 4
The present embodiment provides a kind of chilled poultry storage and transportation freshness safety warning cards, and indicator bacteria enrichment liquid is by following mass percent
Each group be grouped as:10. 0 mg Chicken extracts, 5.5 mg sodium chloride, 0.01 mg TTC.According to the above ratio by three kinds of objects
Matter is compounded, and is mixed well to get the indicator bacteria enrichment liquid, is adsorbed in 25cm2Filter paper.
A concentration of the 10 of 1mL3Cfu/mL P. aeruginosas bacterium solution is by closed filling in small plastic bag.With warn card together
It is packaged in closed polybag.
In use, racking the bacterium bag in packaging, so that bacterium solution is evenly distributed on card of warning, be placed in the outer packing of chilled poultry
.
When chilled poultry storage and transportation temperature reaches"Livestock meat cold chain transportation administrative skill specification"(GB/T 28640)Standard, i.e.,
Chilled meat storage and transportation environment reaches 10 DEG C, and indicator bacteria can grow, and card of warning presents red.
Comparative example 1
The present embodiment provides a kind of chilled poultry storage and transportation freshness safety warning cards, and indicator bacteria enrichment liquid is by following mass percent
Each group be grouped as:0 mg Chicken extracts of 7.0-10., 0.01-0.02 mg TTC.Three kinds of substances are carried out according to the above ratio
Compounding, mixes well to get the indicator bacteria enrichment liquid, is adsorbed in 25cm2Filter paper.(Lack sodium chloride)
A concentration of the 10 of 1mL2-103Cfu/mL P. aeruginosas bacterium solution is by closed filling in small plastic bag.With warn card together
It is packaged in closed polybag.
In use, racking the bacterium bag in packaging, so that bacterium solution is evenly distributed on card of warning, be placed in the outer packing of chilled poultry
.
When chilled poultry storage and transportation temperature is more than"Livestock meat cold chain transportation administrative skill specification"(GB/T 28640)Standard, storage
Fortune environment reaches 15 DEG C, and indicator bacteria cannot grow, and red is not presented in card of warning.
Comparative example 2
The present embodiment provides a kind of chilled poultry storage and transportation freshness safety warning cards, and indicator bacteria enrichment liquid is by following mass percent
Each group be grouped as:0 mg Chicken extracts of 7.0-10., 0.04 mg TTC of 4.5-5.5 mg sodium chloride.According to the above ratio will
Three kinds of substances are compounded, and are mixed well to get the indicator bacteria enrichment liquid, are adsorbed in 25cm2Filter paper.(Increase TTC concentration)
A concentration of the 10 of 1mL2-103Cfu/mL P. aeruginosas bacterium solution is by closed filling in small plastic bag.With warn card together
It is packaged in closed polybag.
In use, racking the bacterium bag in packaging, so that bacterium solution is evenly distributed on card of warning, be placed in the outer packing of chilled poultry
.
When chilled poultry storage and transportation temperature is more than"Livestock meat cold chain transportation administrative skill specification"(GB/T 28640)Standard, storage
Fortune environment reaches 15 DEG C, and indicator bacteria cannot grow, and red is not presented in card of warning.
Comparative example 3
The present embodiment provides a kind of chilled poultry storage and transportation freshness safety warning cards, and indicator bacteria enrichment liquid is by following mass percent
Each group be grouped as:0 mg tryptones of 7.0-10., 4.5-5.5 mg sodium chloride, 0.01-0.02 mg TTC.By above-mentioned ratio
Example compounds three kinds of substances, mixes well to get the indicator bacteria enrichment liquid, is adsorbed in 25cm2Filter paper(Chicken is extracted
Object is changed to tryptone).
A concentration of the 10 of 1mL2-103Cfu/mL P. aeruginosas bacterium solution is by closed filling in small plastic bag.With card one of warning
It rises and is packaged in closed polybag.
In use, racking the bacterium bag in packaging, so that bacterium solution is evenly distributed on card of warning, be placed in the outer packing of chilled poultry
.
When chilled poultry storage and transportation temperature is more than"Livestock meat cold chain transportation administrative skill specification"(GB/T 28640)Standard, storage
Fortune environment reaches 15 DEG C, and indicator bacteria cannot grow, and red is not presented in card of warning.
Comparative example 4
The present embodiment provides a kind of chilled poultry storage and transportation freshness safety warning cards, and indicator bacteria enrichment liquid is by following mass percent
Each group be grouped as:0 mg Chicken extracts of 7.0-10., 4.5-5.5 mg sodium chloride, 0.01-0.02 mg TTC.By above-mentioned
Ratio compounds three kinds of substances, mixes well to get the indicator bacteria enrichment liquid, is adsorbed in 25cm2Filter paper.
A concentration of the 10 of 1mL2-103Cfu/mL Escherichia coli bacteria liquids are by closed filling in small plastic bag.With warn card together
It is packaged in closed polybag.(Replace indicator bacteria)
In use, racking the bacterium bag in packaging, bacterium solution is made to be evenly distributed on card of warning, is placed in the outer packing of chilled poultry i.e.
It can.
Within the shelf-life of chilled poultry(0-4 DEG C, 7d), when chilled poultry storage and transportation temperature reaches"Livestock meat cold chain transportation
Administrative skill specification"(GB/T 28640)Standard, i.e., chilled meat central temperature are no more than 0-4 DEG C, and storage and transportation environment is not higher than 7 DEG C,
Indicator bacteria can grow, and card of warning presents red.
It should be noted that herein, the terms "include", "comprise" or its any other variant are intended to non-row
His property includes, so that the process, method, article or equipment including a series of elements includes not only those elements, and
And further include other elements that are not explicitly listed, or further include for this process, method, article or equipment institute it is intrinsic
Element.
Principle and implementation of the present invention are described for specific case used herein, the explanation of above example
It is merely used to help understand the method and its core concept of the present invention.The above is only a preferred embodiment of the present invention, it answers
When pointing out due to the finiteness of literal expression, and objectively there is unlimited concrete structure, for the common skill of the art
For art personnel, without departing from the principle of the present invention, several improvement, retouching or variation can also be made, can also incited somebody to action
Above-mentioned technical characteristic is combined in the right way;These improve retouching, variation or combination, or the not improved structure by invention
Think and technical solution directly applies to other occasions, is regarded as protection scope of the present invention.
Claims (10)
1. a kind of chilled poultry storage and transportation freshness safety warning card, which is characterized in that card of warning is sealed in container, including absorption
The bacterium solution packet of the filter paper and sealing of colour developing enrichment liquid.
2. a kind of chilled poultry storage and transportation freshness safety warning card according to claim 1, which is characterized in that the filter paper
Using 25cm2Middling speed qualitative filter paper.
3. a kind of chilled poultry storage and transportation freshness safety warning card according to claim 1, which is characterized in that the colour developing
Enrichment liquid is Chicken extract, sodium chloride, 2,3, 5-Triphenyltertrazoliumchloride are dissolved in the water to obtain mixed liquor in proportion.
4. a kind of chilled poultry storage and transportation freshness safety warning card according to claim 1, which is characterized in that the bacterium solution
Packet is the pseudomonas aeruginosa of quantitative filling, bacteria concentration 102-103cfu/mL。
5. a kind of preparation method of chilled poultry storage and transportation freshness safety warning card according to any one of claims 1-4,
It is characterized in that, includes the following steps:
(A)Bacterium solution packs standby, and pseudomonas aeruginosa is activated with beef extract tryptone culture medium to 109Cfu/mL, 3000r/
Min centrifuges 20min, obtains thalline, sterile saline is added to be diluted to 102-103Cfu/mL, quantitative filling to sterile bag, 10-15
DEG C preserve;
(B)Prepared by the enrichment liquid that develops the color, Chicken extract, sodium chloride, 2,3, 5-Triphenyltertrazoliumchloride are dissolved in proportion
Colour developing enrichment liquid is obtained in water;
(C), impregnate, filter paper be soaked in 3 ~ 10s in the colour developing enrichment liquid, draining, 45-50 DEG C of drying, drying preservation;
(D)The filter paper of above-mentioned gained enrichment liquid containing colour developing and the filling bacterium solution to sterile bag are packaged into same closed appearance by encapsulation
In device, it is ensured that after sealing, remaining filtrated air is grown for indicator bacteria in bag, and box height ensures bacterium solution packet in the complete of storage process
Whole property.
6. a kind of preparation method of chilled poultry storage and transportation freshness safety warning card according to claim 1, feature exist
In, in Chicken extract described in the Chicken extract, small peptide content >=50% of molecular weight≤3000, gross protein >=90%,
Mineral salt >=1%.
7. a kind of preparation method of chilled poultry storage and transportation freshness safety warning card according to claim 1, feature exist
In the Chicken extract obtains as follows:Take the chicken carcasses cleaning after segmentation;Boiling water 10min sterilizations;It is cooled to 55-
60 DEG C, enzymolysis 5-8 h;Remaining substance is through milling treatment of colloid after removing bone;It is concentrated in vacuo to 0.5g/mL;Spray drying obtains
Chicken extract pulvis.
8. a kind of preparation method of chilled poultry storage and transportation freshness safety warning card according to claim 7, feature exist
In the enzymolysis uses enzyme activity for the trypsase of 200kU/g, in solid-liquid ratio 1:3, enzyme concentration 2.8-6.7 kU/g, enzymolysis
It is carried out under conditions of time 5-8 h.
9. a kind of preparation method of chilled poultry storage and transportation freshness safety warning card according to claim 7, feature exist
When, cleaning, ultrasonic wave 440w handles 10min after infiltration.
10. according to a kind of use for chilled poultry storage and transportation freshness safety warning card that claim 1 is stated, which is characterized in that
Before chilled poultry enters finished bin, the closed bacterium solution packet warned in card packaging bag is pinched brokenly, card of warning is sufficiently humidified so as to, and continues to keep sealing
State is placed on outside the packaging bag of chilled poultry, together storage and transportation;Matching used control colour atla is directly printed in packaging;Comparison
Compare the color of colour atla, it can be determined that whether the chilled poultry qualified in storage and transport process.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110129406A (en) * | 2019-05-06 | 2019-08-16 | 上海源本食品质量检验有限公司 | A kind of pseudomonas aeruginosa chromogenic culture medium and the method quickly detected using it |
| CN114252435A (en) * | 2020-09-21 | 2022-03-29 | 湖南洪江嵩云禽业有限公司 | Portable safety warning card for freshness of chilled poultry meat in storage and transportation |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2950202A (en) * | 1958-07-29 | 1960-08-23 | Hygrade Food Products Corp | Meat spoilage indicator |
| US4039386A (en) * | 1976-04-02 | 1977-08-02 | Morinaga Milk Industry Co., Ltd. | Method for determining the viable bacteria count of food and a medium for accomplishing the method |
| EP1251350A1 (en) * | 2001-04-11 | 2002-10-23 | Natalie Chen | Food freshness indicator |
| KR20070100501A (en) * | 2006-04-07 | 2007-10-11 | 가부시키가이샤 에스엔티 | A label for indicating a quality |
| CN106525541A (en) * | 2016-09-20 | 2017-03-22 | 广东东阳光药业有限公司 | Method for detecting cell viability of fresh Cordyceps sinensis |
| CN107703125A (en) * | 2016-08-16 | 2018-02-16 | 江南大学 | A kind of preparation method and applications for the intelligent label for judging the critical freshness of pork |
| CN108037116A (en) * | 2017-11-14 | 2018-05-15 | 中国农业大学 | A kind of microorganism type time-temperature indicator card and its preparation method and application |
-
2018
- 2018-06-29 CN CN201810696787.8A patent/CN108693180B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2950202A (en) * | 1958-07-29 | 1960-08-23 | Hygrade Food Products Corp | Meat spoilage indicator |
| GB902018A (en) * | 1958-07-29 | 1962-07-25 | Hygrade Food Products Corp | Time-temperature indicator |
| US4039386A (en) * | 1976-04-02 | 1977-08-02 | Morinaga Milk Industry Co., Ltd. | Method for determining the viable bacteria count of food and a medium for accomplishing the method |
| EP1251350A1 (en) * | 2001-04-11 | 2002-10-23 | Natalie Chen | Food freshness indicator |
| KR20070100501A (en) * | 2006-04-07 | 2007-10-11 | 가부시키가이샤 에스엔티 | A label for indicating a quality |
| CN107703125A (en) * | 2016-08-16 | 2018-02-16 | 江南大学 | A kind of preparation method and applications for the intelligent label for judging the critical freshness of pork |
| CN106525541A (en) * | 2016-09-20 | 2017-03-22 | 广东东阳光药业有限公司 | Method for detecting cell viability of fresh Cordyceps sinensis |
| CN108037116A (en) * | 2017-11-14 | 2018-05-15 | 中国农业大学 | A kind of microorganism type time-temperature indicator card and its preparation method and application |
Non-Patent Citations (2)
| Title |
|---|
| 孙媛媛 等: "猪肉新鲜度指示卡的研究", 《包装工程》, vol. 34, no. 5, pages 29 - 33 * |
| 王红戟 等: "2, 3, 5-三苯基氯化四氮唑在增菌液中的应用研究", 《疾病监测》, vol. 27, no. 5, pages 400 - 402 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110129406A (en) * | 2019-05-06 | 2019-08-16 | 上海源本食品质量检验有限公司 | A kind of pseudomonas aeruginosa chromogenic culture medium and the method quickly detected using it |
| CN114252435A (en) * | 2020-09-21 | 2022-03-29 | 湖南洪江嵩云禽业有限公司 | Portable safety warning card for freshness of chilled poultry meat in storage and transportation |
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| CN108693180B (en) | 2023-12-08 |
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