CN101297658A

CN101297658A - Method for reducing survival rate of salmonella on egg surface by ozonated water

Info

Publication number: CN101297658A
Application number: CNA2008101109434A
Authority: CN
Inventors: 孙宝忠; 贾英民; 刘俊梅; 赵玉佩; 李海鹏; 种京华; 于辉; 马晓燕; 马爱进; 张守勇
Original assignee: Institute of Animal Science of CAAS
Current assignee: Institute of Animal Science of CAAS
Priority date: 2008-06-18
Filing date: 2008-06-18
Publication date: 2008-11-05

Abstract

The invention discloses specific methods such as raw material request, aqueous medium request, ozone water temperature and immersion time, etc. of a technical method for reducing survival percentage of salmonella on the surfaces of eggs by ozone water. The eggs to be processed in the invention are produced by healthy chicken group without salmonella disease and the eggs are fresh eggs with a guarantee period of no more than 7 days. The aqueous medium for preparing the ozone water meets the national NY5028-2001 water quality requirement for processing water of pollution-free food poultry product. When an ozonizer is used for preparing the ozone, the performance of the ozonizer can ensure the ozone concentration in the prepared ozone water to achieve above 4mg/L under the condition of 0 to 30 DEG C. The ozone concentration of ozone water which can reduce the survival percentage of the salmonella on the surfaces of the eggs by above 90 percent is higher than 2mg/L with the optimum temperature of 0 to 5 DEG C. Under the condition of 0 to 30 DEG C, the eggs are soaked in the ozone water with the ozone concentration of above 2mg/L for 10 minutes and then taken out; under the clean condition with the temperature of 20 plus or minus 5 DEG C and the relative humidity of below 30 to 50 percent, the eggs are treated with natural drying or air seasoning and packaged, and then the egg product, the survival percentage of the salmonella on the surface of which being reduced by above 90 percent, is obtained. The methods of the invention can be used for improving the safe mass of the eggs and have broad application value.

Description

A kind of method of reducing survival rate of salmonella on egg surface by ozonated water

Technical field

The present invention relates to a kind of reducing survival rate of salmonella on egg surface by ozonated water, improve the technical method of egg safe mass.

Background technology

The salmonella bacterial type is various, and is widely distributed, is the pathogen of important zoonosis.The salmonellosis that it causes still all is one of food origin disease of the most frequent report in developing country in developed country, and in China, salmonella food poisoning accounts for the ratio Ceng Gaoda 70%～80% of food posioning.In view of this, how to prevent and eliminate food salmonella-polluted is that countries in the world government shows great attention to major issue always.Containing multiple rich nutrient contents in the livestock products such as meat, egg, milk, be suitable for very much the growth and breeding of salmonella, is the major product that causes salmonella poisoning.Because salmonella is to be the common pathogen in animal, birds, insect and the mankind's the enteron aisle, it both can cause retaining in the egg salmonella by sick fowl influence, also easily pollute the egg table just by ight soil and environmental contaminants, and in the egg storage process, enter in the egg, this makes prevention and elimination egg surface receive much attention more with inner salmonella-polluted.In this regard, proposed " cause of disease-food " combination of 21 kinds of needs assessments in 1999 in the 32nd CCFH, and determined 5 groups of preferential evaluation objects, comprising poultry, egg-salmonella; U.S. food and FAD (FDA) strict regulations matter in egg must not dye germ microorganisms such as salmonella is arranged, and needs just can sell behind the detected no salmonella at Canadian egg.In addition, for ensureing egg consumer physical and mental health, it is salmonella-polluted with control egg inside that developed countries such as state such as the U.S., Australia, Japan take system's measure to improve the cluster health quality on the one hand, on the other hand also the bright egg of strict regulations all must through cleaning, sterilization, dry, film, the processing of classification package processing, the just allowance that meets the egg product sanitary standard comes into the market, and the control of the temperature of whole storage, processing and sales process is very strict, and has carried out trade-mark administration.The utilization of these measures and improved the little living safe mass level of these national eggs significantly, but because salmonella exists in extensive rangely, and it is numerous to pollute egg food channel, in the world wide, food poisoning still has report repeatly due to the consumption band shell egg.Cause the risk assessment forecast model estimation of human salmonellosis about consumption shell egg egg according to United States Department of Agriculture: 417 * 1010 eggs are consumed with the shell egg form in annual 615 * 1010 eggs producing of the U.S., wherein have about 2.13 * 106 eggs to be polluted [49] by Bacterium enteritidis.The egg infection rate that Spain produces is up to 12.5%.Be prevention birds, beasts and eggs consumption salmonella poisoning, in December calendar year 2001, edible egg takes place and causes salmonella typhimurium bacteriophage 135a type gastroenteritis in the Australia south, and causes the cross-infection of other foods; In October, 2002, Britain public health laboratory service department data show, contain salmonella in the egg of nearly 3 years cause Spain import, cause 250 people to infect salmonella enteritis, and 10 people are in hospital, 2 people's death; The data of announcing according to CDC shows that during 1990～calendar year 2001, totally 667 of the report SE of various places hygiene department outbursts add up number of the infected 23366 examples.In 309 outbursts that are proved communication media, 241 (78%) is relevant with the feed shell egg, accumulative total 14319 people.Control egg how in full force and effect surface with inner salmonella-polluted be still that show great attention to countries in the world, major issue to be solved arranged.

Since the reform and opening-up, China's egg production development is rapid, promptly is in the first in the world egg egg big producing country status before 20 years.Not only egg output in 2005 accounts for the whole world 42% at present; the egg occupancy volume reaches 20.42 kilograms per capita, surpasses world average level, and in recent years; be accompanied by the propelling of egg production scaleization, standardization, branding, China's birds, beasts and eggs safe and sanitary quality has been able to obvious raising.But, be subjected to China's birds, beasts and eggs modern production developing history of short duration, especially lack influences such as being suitable for egg salmonella disinfecting technical, the consumption of the bright egg of China mainly is to be that main situation is not able to right-about as yet in " dirty egg " mode, according to investigation such as Liu Junmei, " dirty egg " ratio is 37.6% up to pollution rate in the commercially available bright egg of China.。Have ight soil, blood cake, feather, dust etc. to be rich in the pathogenic bacteria pollutant because dirty egg egg table pollutes, China's egg safe and sanitary quality is had a strong impact on, and especially salmonella safe and sanitary quality also is in comparatively low state.According to becoming Daiei etc., Suzhou City in 2004 five batches of egg salmonellas recall rate is up to 80%, and the salmonella recall rate is respectively up to 10% and 11.25% in bright egg and the commodity egg; Liu Junmei etc. also reported in five kinds of commercially available brand eggs of Beijing in 2008, a kind of salmonella that do not detect is only arranged, and other brand egg eggshell salmonella recall rates reach 1.67～8.3%, and its corresponding matter in egg recall rate reaches 1.67～5% in storage process.The China Zhao Zhi of Disease Control and Prevention Center crystalline substance etc. has carried out the research of national science and technology special topic for tackling key problems at salmonella-polluted model of China's egg and harmfulness, think that the quantity that the bright egg of band shell of the annual consumption of China polluted salmonella is higher approximately 100 times than the U.S., annual because of eating the number average out to 5.13 * 10 that is caused salmonellosis by salmonella-polluted band shell aquatic foods egg ⁷The people.Ozone is a kind of internationally recognized antiseptic sterilization agent.It has disinfection high efficiency, noble and unsullied type, broad spectrum activity, economical characters.At present relevant ozone generator to be being widely used in domestic and international drinking-water, swimming with water sterilization, medical apparatus sterilizing and fruit, fishes and shrimps fresh-keeping aspect, but the sterilization of egg salmonella kill aspect effect and application process both at home and abroad also shortage study.In view of this, for improving the egg security, the Eleventh Five-Year Plan period, we are in carrying out national food science and technology support " liquid egg process technology exploitation and industrialization demonstration " the project subject study course of work, system research ozone sterilization to egg safety and quality influence, and then formed this patent.

Summary of the invention

The purpose of this invention is to provide a kind of technical method that can be used to reduce the Ozone Water disinfection of survival rate of salmonella on egg surface.

The technical method that ozone provided by the present invention reduces survival rate of salmonella on egg surface comprises the steps:

1) the raw material egg requires: the shelf-life that egg that the present invention handles should come from no salmonella disease healthy chicken flock output, pollutant such as ight soil, feather, blood cake was arranged with interior, egg surface at 7 days but the egg content salmonella still is in the egg of the state of not detecting.

2) aqueous medium of preparation Ozone Water should be compound national NY 5028-2001 pollution-free food animal products processing water water quality requirement.

3) ozone generator should be able to make under 0～30 ℃ of condition that ozone concentration reaches more than the 4mg/L in the Ozone Water of preparation.Can reduce the Ozone Water ozone concentration of egg surface contamination salmonella survival rate more than 90% and should be higher than 2mg/L, optimum temperature is 0～5 ℃.

4) under 0～30 ℃ of condition, egg is entered immersion taking-up after 10 minutes in the Ozone Water of ozone concentration more than 2mg/L, air dry or air-dry packing get final product under 20 ± 5 ℃ of temperature, relative humidity 30～50% following cleaning conditions.

5) handle egg through the present invention, its surperficial salmonella survival rate should reduce more than 90%.

The specific embodiment

Ozone Water reduces the short motility rate method of salmonella on egg surface under 1. 0～5 ℃ of conditions of embodiment

Opening ozone generator makes temperature in advance reduce to that ozone concentration reaches 4mg/L in 0～5 ℃ the aqueous medium, under 0～5 ℃ of condition, egg is soaked in 10min in the Ozone Water then, take out egg then, drenching that solid carbon dioxide divides and place air dry under 20 ± 5 ℃ of temperature, relative humidity 30～50% following cleaning conditions can make eggshell surface pollution salmonella survival rate is 0% clean egg.

Ozone Water reduces the short motility rate method of salmonella on egg surface under 2,20 ± 5 ℃ of conditions of embodiment

Open ozone generator and make that ozone concentration reaches 4mg/L in the aqueous medium of 20 ± 5 ℃ of temperature, at room temperature egg is soaked in 10min in the Ozone Water then, take out egg then, drench solid carbon dioxide and divide and place under 20 ± 5 ℃ of temperature, relative humidity 30～50% cleaning conditions air dry can make eggshell surface and pollute the salmonella survival rate and reduce to more than 90%.

Ozone Water reduces the short motility rate method of salmonella on egg surface under 3,30 ± 5 ℃ of conditions of embodiment

Open ozone generator and make that ozone concentration reaches 6mg/L in the aqueous medium of 20 ± 5 ℃ of temperature, under 30 ± 5 ℃ of conditions, egg is soaked in 10min in the Ozone Water then, take out egg then, drench solid carbon dioxide and divide and place under 20 ± 5 ℃ of temperature, relative humidity 30～50% following cleaning conditions air dry can make eggshell surface pollution salmonella survival rate and reduce to more than 90%.

Claims

1. a reducing survival rate of salmonella on egg surface by ozonated water technical method operating procedure comprises:

1) shelf-life that egg that the present invention handles should come from no salmonella disease healthy chicken flock output existed salmonella-polluted with interior, surface at 7 days, but egg content is in the bright egg of the state of not detecting.

2) utilize the water that meets national NY 5028-2001 pollution-free food animal products processing water water quality requirement to prepare Ozone Water for medium

3) use the ozone generator that ozone concentration reaches more than the 4mg/L in the Ozone Water that makes preparation under 0 ~ 30 ℃ of condition to prepare Ozone Water.

4) can reduce the Ozone Water ozone concentration of egg surface contamination salmonella survival rate more than 90% and should be higher than 2mg/L, optimum temperature is 0 ~ 5 ℃.

5) under 0 ~ 30 ℃ of condition, egg is immersed in the Ozone Water of ozone concentration more than 2mg/L, soak after 10 minutes and take out, air dry or air-dry packing get final product under 20 ± 5 ℃ of temperature, relative humidity 30 ~ 50% following cleaning conditions.

2. according to the described method of claim 1, the shelf-life that egg that the present invention handles should come from no salmonella disease healthy chicken flock output 7 days with interior, the surface exists salmonella-polluted but egg content is in the bright egg of the state of not detecting.

3. according to the described method of claim 1, employed ozone generator should reach that ozone concentration reaches more than the 4mg/L in the Ozone Water that makes preparation under 0 ~ 30 ℃ of condition.

4. according to the described method of claim 1, the aqueous medium of preparation Ozone Water should meet national NY 5028-2001 pollution-free food animal products processing water water quality requirement.

5. according to the described method of claim 1, can reduce the Ozone Water ozone concentration of egg surface contamination salmonella survival rate more than 90% and should be higher than 2mg/L, optimum temperature is 0 ~ 5 ℃.

6. according to the described method of claim 1, under 0 ~ 30 ℃ of condition, egg is immersed immersion taking-up after 10 minutes in the Ozone Water of ozone concentration more than 2mg/L, and air dry or air-dry packing can obtain the product of survival rate of salmonella on egg surface reduction more than 90% under 20 ± 5 ℃ of temperature, relative humidity 30 ~ 50% following cleaning conditions.