CN108690144A - One primary yeast extracts the production method of trehalose - Google Patents
One primary yeast extracts the production method of trehalose Download PDFInfo
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- CN108690144A CN108690144A CN201810774257.0A CN201810774257A CN108690144A CN 108690144 A CN108690144 A CN 108690144A CN 201810774257 A CN201810774257 A CN 201810774257A CN 108690144 A CN108690144 A CN 108690144A
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- China
- Prior art keywords
- trehalose
- production method
- extraction
- primary yeast
- exchange resin
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The present invention relates to trehalose production technical fields, specifically disclose the production method of primary yeast extraction trehalose, include the following steps:Step 1, Saccharomyces cerevisiae boil extraction, step 2, extracting solution enzymatic treatment, step 3, the processing of bulk processing liquid, step 4, the pure liquid processing of trehalose, step 5, supernatant removal of impurities, step 6, trehalose crystallization.The present invention overcomes the deficiencies in the prior art, boil the trehalose in extraction method extraction yeast using distilled water, remove protein using the method that complex enzyme is combined with exchange column, reduce production cost, improve the profit of enterprise, reduce environmental pollution.
Description
Technical field
The present invention relates to trehalose production technical fields, particularly belong to the production method of primary yeast extraction trehalose.
Background technology
Trehalose is also known as Radix Rhapontici seu Radix Echinopsis sugar, gill fungus sugar etc..It is a kind of safe and reliable natural carbohydrate.Trehalose is by two grapes
The nonreducing sugar that glycan molecule is constituted with 1,1- glycosidic bonds has 3 kinds of isomers i.e. trehalose(α, α), isotrehalose(β, β)With it is new
Trehalose(α, β), and there is non-specific protective effect to various bioactivators.Trehalose is many edible in nature
With being all widely present in animals and plants and microbial body.Since the content of trehalose in yeast is larger, the 20% of yeast dry weight can reach
More than, so production trehalose is mainly extracted from yeast now.
Existing yeast extraction trehalose uses the method that ethyl alcohol is heated to reflux, and alkali metal is utilized in ethyl alcohol heating reflux method
Isolating protein can cause environmental pollution, and cost is very high, and protein can not recycle.The cost of ethyl alcohol heating reflux method
Higher, the profit of product is low.
Invention content
The object of the present invention is to provide the production methods that a primary yeast extracts trehalose, overcome the prior art not
Foot boils the trehalose in extraction method extraction yeast using distilled water, egg is removed using the method that complex enzyme is combined with exchange column
White matter reduces production cost, improves the profit of enterprise, reduces environmental pollution.
To solve the above problems, the technical solution used in the present invention is as follows:
One primary yeast extracts the production method of trehalose, includes the following steps:
Step 1, Saccharomyces cerevisiae boil extraction, take suitable Saccharomyces cerevisiae that a certain amount of distilled water is added and are uniformly mixed, are placed in and add
It is boiled in hot pot, continues heating 1-1.5 hours, ceaselessly stirred in heating process, obtain extracting solution;
Papain and trypsase mixed solution is added in step 2, extracting solution enzymatic treatment in extracting solution, and temperature is in 50-
It is handled 20 hours between 60 DEG C, cooling acquisition bulk processing liquid is centrifuged after enzyme deactivation;
Step 3, the processing of bulk processing liquid will pass through pretreated Macroporous weak acid cation exchange resin and fill column, by bulk processing liquid
Isolating protein is further removed with the flow velocity of 1.5mL/s, obtains the pure liquid of trehalose;
Step 4, the pure liquid processing of trehalose, the PH for adjusting the pure liquid of trehalose is 4-6,1% activated carbon is added, under the conditions of 40 DEG C
Water bath with thermostatic control is stirred 0.5-1 hours, until centrifuging and taking supernatant after pure liquid is colourless;
Treated Macroporous strongly acid cation exchange resin and macroporous strong basic resin anion (R.A.) are pressed in step 5, supernatant removal of impurities
According to volume ratio 2:1 mixing dress column, supernatant carry out cation and anion exchange impurity elimination with the flow velocity of 1.5mL/s, are washed with distilled water friendship
Column is changed, eluent is obtained.
Step 6, trehalose crystallization will obtain trehalose crystallization after eluent concentration, crystallization, drying.
Further, a large amount of vapor is will produce when being heated in the step 1 in heating kettle, after collecting vapor condensation
It flows back into heating kettle.
Further, in the step 2 papain and trypsase a concentration of 55-60%, enzyme-removal temperature 110-
120℃。
Further, the models such as macroporous ion exchange resin optional D151, D152, D113 in the step 3, warp
The best results of overtesting D152 models.
Further, 001X7 or 001X1.1 may be selected in the storng-acid cation exchange resin of the use, and strong basicity is cloudy
201X7 or 201X4 may be selected in ion exchange resin.
Further, eluent is concentrated into 3 times of volumes of addition in the concentrate of mass fraction 30%-40% in the step 5
Absolute ethyl alcohol, then stirred evenly under the conditions of 50 DEG C, seaweed sugar crystal be dried to obtain after filtering.
Further, the purity of the seaweed sugar crystal prepared is more than 98.26%, and the recovery rate of trehalose can reach 95.2%
More than.
Compared with prior art, implementation result of the invention is as follows by the present invention:
The production method of primary yeast extraction trehalose of the present invention boils the sea in extraction method extraction yeast using distilled water
Algae sugar removes protein using the method that complex enzyme is combined with exchange column, reduces production cost, improve the profit of enterprise,
Reduce environmental pollution.
Specific implementation mode
With reference to embodiment, the invention will be further described, but the present invention is not limited to these instances, and is being de-
Under the premise of from present inventive concept, carried out by it is any improvement be within the scope of the present invention.
Embodiment 1
The production method of primary yeast extraction trehalose of the present invention, includes the following steps:
Step 1, Saccharomyces cerevisiae boil extraction, take suitable Saccharomyces cerevisiae that a certain amount of distilled water is added and are uniformly mixed, are placed in and add
It is boiled in hot pot, continues heating 1-1.5 hours, ceaselessly stirred in heating process, obtain extracting solution;
Papain and trypsase mixed solution is added in step 2, extracting solution enzymatic treatment in extracting solution, and temperature is in 50-
It is handled 20 hours between 60 DEG C, the cooling acquisition pure liquid of trehalose is centrifuged after enzyme deactivation;
Step 3, the pure liquid processing of trehalose, the PH for adjusting the pure liquid of trehalose is 4-6,1% activated carbon is added, under the conditions of 40 DEG C
Water bath with thermostatic control is stirred 0.5-1 hours, until centrifuging and taking supernatant after pure liquid is colourless;
Treated Macroporous strongly acid cation exchange resin and macroporous strong basic resin anion (R.A.) are pressed in step 4, supernatant removal of impurities
According to volume ratio 2:1 mixing dress column, supernatant carry out cation and anion exchange impurity elimination with the flow velocity of 1.5mL/s, are washed with distilled water friendship
Column is changed, eluent is obtained.
Step 5, trehalose crystallization will obtain trehalose crystallization after eluent concentration, crystallization, drying.
Further, a large amount of vapor is will produce when being heated in the step 1 in heating kettle, after collecting vapor condensation
It flows back into heating kettle.
Further, in the step 2 papain and trypsase a concentration of 55-60%, enzyme-removal temperature 110-
120℃。
Further, 001X7 or 001X1.1 may be selected in the storng-acid cation exchange resin of the use, and strong basicity is cloudy
201X7 or 201X4 may be selected in ion exchange resin.
Further, eluent is concentrated into 3 times of volumes of addition in the concentrate of mass fraction 30%-40% in the step 5
Absolute ethyl alcohol, then stirred evenly under the conditions of 50 DEG C, seaweed sugar crystal be dried to obtain after filtering.
Further, the purity of the seaweed sugar crystal prepared is 93.24%, the extraction rate reached of trehalose to 94.2%.
Embodiment 2
The production method of primary yeast extraction trehalose of the present invention, includes the following steps:
Step 1, Saccharomyces cerevisiae boil extraction, take suitable Saccharomyces cerevisiae that a certain amount of distilled water is added and are uniformly mixed, are placed in and add
It is boiled in hot pot, continues heating 1-1.5 hours, ceaselessly stirred in heating process, obtain extracting solution;
Papain and trypsase mixed solution is added in step 2, extracting solution enzymatic treatment in extracting solution, and temperature is in 50-
It is handled 20 hours between 60 DEG C, cooling acquisition bulk processing liquid is centrifuged after enzyme deactivation;
Step 3, the processing of bulk processing liquid will pass through pretreated Macroporous weak acid cation exchange resin and fill column, by bulk processing liquid
Isolating protein is further removed with the flow velocity of 1.5mL/s, obtains the pure liquid of trehalose;
Step 4, the pure liquid processing of trehalose, the PH for adjusting the pure liquid of trehalose is 4-6,1% activated carbon is added, under the conditions of 40 DEG C
Water bath with thermostatic control is stirred 0.5-1 hours, until centrifuging and taking supernatant after pure liquid is colourless;
Treated Macroporous strongly acid cation exchange resin and macroporous strong basic resin anion (R.A.) are pressed in step 5, supernatant removal of impurities
According to volume ratio 2:1 mixing dress column, supernatant carry out cation and anion exchange impurity elimination with the flow velocity of 1.5mL/s, are washed with distilled water friendship
Column is changed, eluent is obtained.
Step 6, trehalose crystallization will obtain trehalose crystallization after eluent concentration, crystallization, drying.
Further, a large amount of vapor is will produce when being heated in the step 1 in heating kettle, after collecting vapor condensation
It flows back into heating kettle.
Further, in the step 2 papain and trypsase a concentration of 55-60%, enzyme-removal temperature 110-
120℃。
Further, the models such as macroporous ion exchange resin optional D151, D152, D113 in the step 3, warp
The best results of overtesting D152 models.
Further, 001X7 or 001X1.1 may be selected in the storng-acid cation exchange resin of the use, and strong basicity is cloudy
201X7 or 201X4 may be selected in ion exchange resin.
Further, eluent is concentrated into 3 times of volumes of addition in the concentrate of mass fraction 30%-40% in the step 5
Absolute ethyl alcohol, then stirred evenly under the conditions of 50 DEG C, seaweed sugar crystal be dried to obtain after filtering.
Further, the purity of the seaweed sugar crystal prepared is more than 98.26%, and the recovery rate of trehalose can reach 95.2%
More than.
Above content is only to present inventive concept example and explanation, affiliated those skilled in the art couple
Described specific embodiment does various modifications or additions or substitutes by a similar method, without departing from invention
Conceive or beyond the scope defined by this claim, is within the scope of protection of the invention.
Claims (7)
1. a primary yeast extracts the production method of trehalose, it is characterised in that:Include the following steps:
Step 1, Saccharomyces cerevisiae boil extraction, take suitable Saccharomyces cerevisiae that a certain amount of distilled water is added and are uniformly mixed, are placed in and add
It is boiled in hot pot, continues heating 1-1.5 hours, ceaselessly stirred in heating process, obtain extracting solution;
Papain and trypsase mixed solution is added in step 2, extracting solution enzymatic treatment in extracting solution, and temperature is in 50-
It is handled 20 hours between 60 DEG C, cooling acquisition bulk processing liquid is centrifuged after enzyme deactivation;
Step 3, the processing of bulk processing liquid will pass through pretreated Macroporous weak acid cation exchange resin and fill column, by bulk processing liquid
Isolating protein is further removed with the flow velocity of 1.5mL/s, obtains the pure liquid of trehalose;
Step 4, the pure liquid processing of trehalose, the PH for adjusting the pure liquid of trehalose is 4-6,1% activated carbon is added, in 40 DEG C of conditions
Lower water bath with thermostatic control is stirred 0.5-1 hours, until centrifuging and taking supernatant after pure liquid is colourless;
Treated Macroporous strongly acid cation exchange resin and macroporous strong basic resin anion (R.A.) are pressed in step 5, supernatant removal of impurities
According to volume ratio 2:1 mixing dress column, supernatant carry out cation and anion exchange impurity elimination with the flow velocity of 1.5mL/s, are washed with distilled water friendship
Column is changed, eluent is obtained;
Step 6, trehalose crystallization will obtain trehalose crystallization after eluent concentration, crystallization, drying.
2. the production method of primary yeast extraction trehalose according to claim 1, it is characterised in that:In the step 1
A large amount of vapor is will produce when heating in heating kettle, is flowed back into heating kettle after collecting vapor condensation.
3. the production method of primary yeast extraction trehalose according to claim 1, it is characterised in that:In the step 2
A concentration of 55-60% of papain and trypsase, enzyme-removal temperature are 110-120 DEG C.
4. the production method of primary yeast extraction trehalose according to claim 1, it is characterised in that:In the step 3
The models such as macroporous ion exchange resin optional D151, D152, D113, the best results through overtesting D152 models.
5. the production method of primary yeast extraction trehalose according to claim 1, it is characterised in that:The use it is strong
Acid cation exchange resin may be selected 001X7 either 001X1.1 strong-base anion-exchange resins may be selected 201X7 or
201X4。
6. the production method of primary yeast extraction trehalose according to claim 1, it is characterised in that:In the step 5
Eluent is concentrated into the absolute ethyl alcohol that 3 times of volumes are added in the concentrate of mass fraction 30%-40%, then is stirred under the conditions of 50 DEG C
It mixes uniformly, seaweed sugar crystal is dried to obtain after filtering.
7. the production method of primary yeast extraction trehalose according to claim 1, it is characterised in that:The sea prepared
The purity of algae sugar crystal is more than 98.26%, and the recovery rate of trehalose can reach 95.2% or more.
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| CN201810774257.0A CN108690144A (en) | 2018-07-16 | 2018-07-16 | One primary yeast extracts the production method of trehalose |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110229854A (en) * | 2019-06-24 | 2019-09-13 | 石狮市华宝明祥食品有限公司 | A kind of algae beverage processing biological enzyme processing method |
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2018
- 2018-07-16 CN CN201810774257.0A patent/CN108690144A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110229854A (en) * | 2019-06-24 | 2019-09-13 | 石狮市华宝明祥食品有限公司 | A kind of algae beverage processing biological enzyme processing method |
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Application publication date: 20181023 |