CN108686052A - It is a kind of to adjust endocrine anti-aging biological agent, preparation method and applications - Google Patents
It is a kind of to adjust endocrine anti-aging biological agent, preparation method and applications Download PDFInfo
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Abstract
Endocrine anti-aging biological agent is adjusted the invention discloses a kind of, it is composed of the following components to prepare raw material:30-50 parts of grape, 20-30 parts of granatum, 10-15 parts of Radix Astragali, 5-10 parts of Radix Angelicae Sinensis, Jiangnan is Hu 10-20 parts profound, 5-10 parts of Mucuna birdwoodiana.In addition, the present invention also provides a kind of preparation method and application adjusting endocrine anti-aging biological agent.The present invention improves internal system using the synergistic effect of all medicines, by disperse blood stasis and dredge collateral, improves cycle, eliminates siltation in vivo, adjustment incretion balance to realize, makes human body functional recovery to good physiological status.In addition, the biological agent of the present invention can also anti-ageing ecchymose removing, improve that skin is yellow, black, relaxed state, desalinate melanin, supplement a variety of nutriments of skin, desalination wrinkle has the foreground of being widely applied.
Description
Technical field
The invention belongs to pharmaceutical technology fields, and in particular to a kind of to adjust endocrine anti-aging biological agent and preparation side
Method.
Background technology
Human body has internal system, secretes metabolism and physiological function that various hormones and nervous system adjust human body together.
Various hormones are to maintain balance under normal circumstances, such as because certain reason makes this balance break (certain hormonosis or mistake
It is few), this results in endocrine disorder, can cause corresponding clinical manifestation.Endocrine disorder causes prodigious puzzlement, example to women
Such as, cutaneous malignancies can be caused, many macula luteas, color spot, butterfly macules, melanin deposition etc. are will appear on skin, even if smearing cosmetics
Also of no avail.For another example endocrine disorder can cause many gynecological diseases, as endometriosis, menstrual flow it is irregular,
Dysmenorrhoea, irregular menstruation etc. can also cause infertile what is more.Thus it is guaranteed that incretion balance is extremely important.
For endocrine imbalance, the guideline of doctor trained in Western medicine is to adjust endocrine, is allowed to reach balance again.For
The different cause of disease, symptom and constitution, severity, have different countermeasures.Work(caused by usually excessive to hormone secretion
Can be hyperfunction, to inhibit, cut down as principle, operation excision endocrine tumors can be taken, or with the secretion of drug inhibitory hormone with
Synthesis;Imbalance caused by very few to hormone secretion is essentially its deficiency of supplement, including supplement physiological dose hormone, organ shifting
Plant etc..But there is many side effects for the use of hormone, such as the hyperfunction syndrome of class adrenal cortex function occur, have floating
Swollen, Diagnostic value, hypertension, glycosuria, thinning of skin, moon shaped face, buffalo hump, central obesity, crinosity, acne, myasthenia and flesh
The symptoms such as atrophy.
From the point of view of in Chinese medicine, endocrine disorder is the performance of the deficiency of Yin, is caused by being retarded by silt by qi and blood.Extravasated blood be detained in vivo,
Train of thought, which is obstructed, outer poison invades human body, lochiostasis etc. may all be led to that qi and blood is retarded by silt.Many women common diseases, in fact
All it is caused by endocrine disorder.Therefore, these illnesss are treated, to be started with from endocrine is adjusted, is enabled qi and blood unobstructed, make essence
Blood nourishes whole body, promotes blood circulation, comprehensive conditioning from inside to outside.
Invention content
Endocrine anti-aging biological agent is adjusted the present invention provides a kind of, solves and is controlled in the prior art using doctor trained in Western medicine
Treat the big problem of endocrine disorder side effect.
Adjusting endocrine anti-aging biological agent the first purpose of the invention is to provide a kind of, prepare raw material by with
The group of lower parts by weight is grouped as:30-50 parts of grape, 20-30 parts of granatum, 10-15 parts of Radix Astragali, 5-10 parts of Radix Angelicae Sinensis, Jiangnan are profound
10-20 parts of Hu, 5-10 parts of Mucuna birdwoodiana.
Preferably, the raw material for preparing for adjusting endocrine anti-aging biological agent is grouped by the group of following parts by weight
At:40 parts of grape, 25 parts of granatum, 10 parts of Radix Astragali, 10 parts of Radix Angelicae Sinensis, 15 parts of the profound Hu in Jiangnan, 5 parts of Mucuna birdwoodiana.
Second object of the present invention is to provide a kind of preparation method adjusting endocrine anti-aging biological agent, including
Following steps:
Step 1, weigh in parts by weight 30-50 parts of grape, 20-30 parts of granatum, 10-15 parts of Radix Astragali, 5-10 parts of Radix Angelicae Sinensis,
Jiangnan is Hu 10-20 parts profound, 5-10 parts of Mucuna birdwoodiana, spare;
Step 2, the fruit pulp that grape is weighed in step 1 is detached with grape pip, respectively obtain grape pomace pulp and
Grape pip;
The mashing of grape pomace pulp is broken, obtain grape slurries;
It pulverizes after grape pip is dried, obtains grape pip powder;
Step 3, the water for being equivalent to 10 times of granatum weight is added into the granatum that step 1 weighs, is smashed to pieces and is slurried
Body obtains pomegranate latex, and the pH for adjusting pomegranate latex is 5.0-6.0, and is heated to 45-50 DEG C, is then charged with quite
In the complex enzyme of pomegranate latex weight 0.1%, 5h is digested, obtains enzymolysis material;
The grape slurries obtained in step 2 are uniformly mixed with enzymolysis material, obtain mixed fermentation raw material, it is former toward mixed fermentation
The S. cervisiae bacterium solution for being equivalent to mixed fermentation raw material weight 1% is added in material, is sealed after stirring evenly, at 30-35 DEG C
Fermentation 15 days, fermentation finish to obtain tunning, tunning are filtered, ferment filtrate is obtained;
Step 4, one is blended in after the Radix Astragali weighed in step 1, Radix Angelicae Sinensis, the profound Hu in Jiangnan and Mucuna birdwoodiana being crushed
It rises, is then charged with the ethanol solution for being equivalent to Radix Astragali, 5 times of Radix Angelicae Sinensis, the profound Hu in Jiangnan and Mucuna birdwoodiana total weight amounts
2h is impregnated, reheats refluxing extraction 3 times, 2h is extracted every time, merges all extracting solutions, is filtered, filtrate concentrates removing at 80 DEG C
Ethyl alcohol obtains Chinese medicine extract;
Step 5, the grape pip powder in step 2, the ferment filtrate in step 3 and the Chinese medicine extract in step 4 are mixed
It closes uniformly, it is 1.2-1.3 to be then concentrated under reduced pressure into density, obtains medicinal extract;By medicinal extract in 60 DEG C, the item that vacuum degree is -0.08Mpa
It is dried in vacuo under part, obtains dry extract, the dry extract is to adjust endocrine anti-aging biological agent.
Preferably, pharmaceutically acceptable pharmaceutical preparation is conventionally made in the dry extract.
Preferably, the pharmaceutical dosage form of the pharmaceutical preparation is tablet, capsule, granule, Disket or pill.
Preferably, the granularity of grape pip powder is 20 mesh in the step 2.
Preferably, the complex enzyme is by pectase, cellulase and carbohydrase according to 2:2:1 quality compares mixed preparing
It forms.
Preferably, the pectinase activity, the cellulase activity, the saccharifying enzymic activity are 10000U/mL.
Preferably, living bacteria count is 10 in the S. cervisiae bacterium solution8-1010cfu/mL。
Third object of the present invention is to provide a kind of endocrine anti-aging biological agents of adjusting described in claim 1
Preparing the application in adjusting endocrine, antiaging agent.
Side's solution of the present invention:
Grape:Acid mild-natured, sweet in flavor, enters lung, spleen, kidney channel, fill blood, liver and kidney benefiting, born fluid, strengthening the bones and muscles, cough-relieving relieving restlessness,
The effect of tonifying Qi and blood, tonneau urine.The procyanidin content in resveratrol, grape pip in Grape Skin is all higher than grape
Other positions, also above other most of fruit trees, and have high medical value.The study found that grape is than aspirin energy
Thrombosis is preferably prevented, and human serum cholesterol levels can be reduced, reduces the cohesiveness of blood platelet, to preventing heart and brain blood
Pipe disease has certain effect.Suitable fresh grape is eaten daily, can not only be reduced the onset risk of angiocardiopathy, is additionally beneficial to office
The health of portion's ischemic heart disease and atherosclerotic heart disease patient.Flavonoid substances in fresh grape, can " cleaning " blood
Liquid prevents the formation of cholesterol plaques.
Granatum:Property acid, taste it is astringent, have hemostasis, puckery intestines, desinsection effectiveness.Contain a large amount of granatums in granatum
Polyphenol, pomegranate peel polyphenol are a kind of general names with polyphenol hydroxyl compound, including nutgall tannin, Ellagitannins, ellagic acid,
The multiple compounds such as chlorogenic acid, gallic acid, catechin, epicatechin, anthocyanidin, ferulic acid and quercetin, content are about that it is dry
The 10-20% of weight.Numerous studies show that pomegranate peel polyphenol has Green Tea Extract, anti-mutagenesis, anti-aging, antibacterial, prevention of cardiovascular
Disease improves the multiple functions such as women's climacteric syndrome.
Radix Astragali:It is sweet, warm.Have the function of invigorating qi for consolidating superficies, arrest sweating admittedly take off, torr sore myogenic, inducing diuresis for removing edema.Contain Huang in Radix Astragali
Ketone, saponin constituents, flavones ingredient such as formononetin, 3'Hydroxyl formononetin (calycosin) and its glucose
Glucoside, 2', 3'Dihydroxy -7,4'Dimethoxy isoflavones, 7,2'Dihydroxy -3', 4'The different Radix Astragali flavane of dimethoxy and its
Glycoside, 7,3'Dihydroxy -4', 5'Dimethoxy isoflavan, 3-hydroxy-9,10-dimethoxy pterocarpane and its grape
Glucoside etc., some of which ingredient have stronger antioxidant activity, moreover it is possible to enhance body's immunity, enhancing hematopoiesis function, improve
The effects that metabolism, enhancing gonad function, resisting stress, anti-aging.Radix Astragali also has cardiac stimulant, adjusts blood pressure, antiviral property
The effects that myocarditis, liver protection, antiulcer.
Radix Angelicae Sinensis:It is sweet, pungent, warm.Have effects that enrich blood, invigorate blood circulation, menstruction regulating and pain relieving, moisturize laxation.Radix Angelicae Sinensis polysaccharide can be by straight
Macrophage, the lymphocyte etc. activated with degradation pathways in hematopoieticmicroenviron-ment is connect, muscular tissue can be also stimulated, its generation is promoted to make
Blood regulatory factor, and then promotion functions candidate stem cell granular leukocyte macrophage colony stimulates marrow at the Proliferation, Differentiation of unit
Grain monosystem hematopoiesis.
The profound Hu in Jiangnan:It is bitter, mild-natured.Have effects that removing toxicity for detumescence, dissipate the stasis of blood, promoting blood circulation and stopping pain.Contain in the profound Hu in Jiangnan
Globulol, γ-gurjunene, terpinen-4-ols, nopinone etc. have effects that the scattered stasis of blood, dissipating bind, have very for vivotoxin siltation
Good dissipation role promotes body cycle unobstructed, prevents siltation from forming lump or knurl.
Mucuna birdwoodiana:Mucuna birdwoodiana be pulse family Mucuna plant, wherein containing 3- phenyl coumarin classes compound,
2- phenyl benzofurans analog derivative, isoflavones, red sandalwood vinyl compound, anthraquinone analog compound, triterpene compound have and mend
The effect of blood, degrading the channel, strengthening the bones and muscles, is mainly used for anaemia, leukopenia, irregular menstruation, the diseases such as pain in waist and lower extremities.
Compared with prior art, the beneficial effects of the present invention are:
1) present invention improves internal system using the synergistic effect of all medicines, by disperse blood stasis and dredge collateral, improves cycle, to
It realizes and eliminates siltation in vivo, adjustment incretion balance, make human body functional recovery to good physiological status.In addition, the life of the present invention
Object preparation can also anti-ageing ecchymose removing, improve that skin is yellow, black, relaxed state, desalinate melanin, the supplement a variety of nutriments of skin,
Desalinate wrinkle.
2) present invention is extracted the active ingredient in grape and granatum using enzymolysis and zymotechnique to greatest extent
Come, using the Punica granatum L. polyphenol collective effect in flavonoid substance in grape and granatum, play disperse blood stasis and dredge collateral, improve cycle and
Green Tea Extract, anti-mutagenesis, anti-aging function are lacked of proper care so as to improve human endocrine.
Specific implementation mode
In order to enable those skilled in the art to more fully understand, technical scheme of the present invention is practiced, with reference to specific
The invention will be further described for embodiment, but illustrated embodiment is not as a limitation of the invention.
S. cervisiae as used in the following examples is specially that China Committee for Culture Collection of Microorganisms is commonly micro-
Bio-Centers deposit number is the bacterial strain of CGMCC NO.2735.
S. cervisiae bacterium solution is obtained using conventional method culture in embodiment, is tested described in following each embodiments
Method is unless otherwise specified conventional method.
Embodiment 1
It is a kind of to adjust endocrine anti-aging biological agent, it prepares raw material and is grouped as by the group of following parts by weight:Portugal
40 parts of grape, 25 parts of granatum, 10 parts of Radix Astragali, 10 parts of Radix Angelicae Sinensis, 15 parts of the profound Hu in Jiangnan, 5 parts of Mucuna birdwoodiana;
It is specifically implemented according to the following steps:
Step 1, the profound Hu in 40 parts of grape, 25 parts of granatum, 10 parts of Radix Astragali, 10 parts of Radix Angelicae Sinensis, Jiangnan 15 is weighed in parts by weight
Part, 5 parts of Mucuna birdwoodiana, it is spare;
Step 2, the fruit pulp that grape is weighed in step 1 is detached with grape pip, respectively obtain grape pomace pulp and
Grape pip;
The mashing of grape pomace pulp is broken, obtain grape slurries;
It pulverizes after grape pip is dried, crosses 20 mesh sieve, obtain grape pip powder;
Step 3, the water for being equivalent to 10 times of granatum weight is added into the granatum that step 1 weighs, is smashed to pieces and is slurried
Body obtains pomegranate latex, and the pH for adjusting pomegranate latex is 5.0-6.0, and is heated to 45-50 DEG C, is then charged with quite
In the complex enzyme of pomegranate latex weight 0.1%, 5h is digested, obtains enzymolysis material;
The grape slurries obtained in step 2 are uniformly mixed with enzymolysis material, obtain mixed fermentation raw material, it is former toward mixed fermentation
The S. cervisiae bacterium solution for being equivalent to mixed fermentation raw material weight 1% is added in material, is sealed after stirring evenly, at 30-35 DEG C
Fermentation 15 days, fermentation finish to obtain tunning, tunning are filtered, ferment filtrate is obtained;
Wherein, complex enzyme is by pectase, cellulase and carbohydrase according to 2:2:1 quality is formed than mixed preparing,
And pectinase activity, cellulase activity and saccharifying enzymic activity are 10000U/mL;
Living bacteria count is 10 in S. cervisiae bacterium solution8-1010cfu/mL;
Step 4, one is blended in after the Radix Astragali weighed in step 1, Radix Angelicae Sinensis, the profound Hu in Jiangnan and Mucuna birdwoodiana being crushed
It rises, is then charged with the ethanol solution for being equivalent to Radix Astragali, 5 times of Radix Angelicae Sinensis, the profound Hu in Jiangnan and Mucuna birdwoodiana total weight amounts
2h is impregnated, reheats refluxing extraction 3 times, 2h is extracted every time, merges all extracting solutions, is filtered, filtrate concentrates removing at 80 DEG C
Ethyl alcohol obtains Chinese medicine extract;
Step 5, the grape pip powder in step 2, the ferment filtrate in step 3 and the Chinese medicine extract in step 4 are mixed
It closes uniformly, it is 1.2-1.3 to be then concentrated under reduced pressure into density, obtains medicinal extract;By medicinal extract in 60 DEG C, the item that vacuum degree is -0.08Mpa
It is dried in vacuo under part, obtains dry extract, the dry extract is to adjust endocrine anti-aging biological agent;
Will above-mentioned dry extract crush after plus appropriate amount of auxiliary materials, mixing pelletize, be sieved, it is dry, then by particulate matter obtained pass through into
One step tabletting, it is dry, obtain the endocrine anti-aging biological agent of adjusting of tablet form.
Embodiment 2
It is a kind of to adjust endocrine anti-aging biological agent, it prepares raw material and is grouped as by the group of following parts by weight:Portugal
30 parts of grape, 30 parts of granatum, 15 parts of Radix Astragali, 5 parts of Radix Angelicae Sinensis, 10 parts of the profound Hu in Jiangnan, 10 parts of Mucuna birdwoodiana.
The preparation method is the same as that of Example 1, and difference lies in will be formulated the formula for being changed to embodiment 2.
After the dry extract that embodiment 2 is prepared crushes plus appropriate amount of auxiliary materials, mixing are pelletized, and are sieved, dry, are packed into capsule, i.e.,
The endocrine anti-aging biological agent of adjusting of capsule-type is made.
Embodiment 3
It is a kind of to adjust endocrine anti-aging biological agent, it prepares raw material and is grouped as by the group of following parts by weight:Portugal
50 parts of grape, 20 parts of granatum, 12 parts of Radix Astragali, 8 parts of Radix Angelicae Sinensis, 20 parts of the profound Hu in Jiangnan, 8 parts of Mucuna birdwoodiana.
The preparation method is the same as that of Example 1, and difference lies in will be formulated the formula for being changed to embodiment 3.
After the dry extract that embodiment 3 is prepared crushes plus appropriate amount of auxiliary materials, mixing are pelletized, and are sieved, dry, obtain particle
The endocrine anti-aging biological agent of adjusting of type.
Since the endocrine anti-aging biological agent performance of adjusting that embodiment 1-3 is prepared is essentially identical, only with
The endocrine anti-aging biological agent of adjusting that embodiment 1 is prepared is used as explanation.
1, the measurement of the reducing power of endocrine anti-aging biological agent is adjusted
It is measured using Prussia's blue laws, steps are as follows for specific experiment:
Be added into the endocrine anti-aging biological agent of adjusting of 1mg embodiments 1 2.5mL phosphate buffers (pH 6.6,
0.2mo1/L), the K that mass fraction is 1% is added3Fe(CN)6Solution 1mL, is placed in 50 DEG C of water-bath 20min, and ice bath cools down rapidly
The solution of trichloroacetic acid 2.5mL that mass fraction is 10% is added later, supernatant 5mL is taken after centrifuging 10min, 5mL distillations are added
Water adds the ferric chloride solution 1mL that mass fraction is 0.1%, and mixing stands 10min, and light absorption value A, distillation are surveyed at 710nm
Water is blank absorbency A0, potassium ferricyanide reduction rate is calculated as follows.
Reduction rate (%)=(A-A0) × 100%;
Wherein A is sample liquid light absorption value, A0For blank absorbency.
By measuring, the endocrine total reducing power of anti-aging biological agent of adjusting obtained of embodiment 1 is stronger, also proper energy
Power is up to 93.5%.
2, superoxide anion (O2-) Scavenging activity measurement
4.5mL Tris-HCl buffer solutions (25 DEG C, pH8.2,50mmol/L) are put into 25 DEG C of water-bath 20min, 1mg is added
The endocrine anti-aging biological agent of adjusting of embodiment 1, adds 0.4mL pyrogallols solution (25mmol/L), mixing 25
DEG C water-bath 5min is added 1mL HCl solutions (8mmol/L) and surveys absorbance A 325nm at, and distilled water does blank, survey absorbance
A0, it is As that 4.5mL Tris-HCl buffer solutions, which are added 1mL sample liquids and measure control light absorption value,.
Clearance rate (%)=[A-(AS-A0)]/ A × 100%;
Wherein A is sample liquid light absorption value, ASTo compare light absorption value, A0For blank absorbency.
After measured, the endocrine anti-aging biological agent of adjusting obtained of embodiment 1 has centainly superoxide anion
Scavenging activity, Scavenging activity are up to 91.8%.
3, the measurement of endocrine anti-aging biological agent Scavenging ability is adjusted
1mL salicylic acids-ethanol solution (6mmol/L) is added into 1mL ferrous sulfate (6mmol/L), 1mg embodiments are added
The 1 endocrine anti-aging biological agent of adjusting, then add distilled water to 5.0mL, 1mLH is then added2O2, 510nm after 10min
Place surveys light absorption value A, and distilled water is blank absorbency A0。
Clearance rate (%)=(A0-A)/A0× 100%;
Wherein A is sample liquid light absorption value, A0For blank absorbency.
After measured, embodiment 1 is obtained adjusts endocrine anti-aging biological agent to hydroxy radical with centainly clear
Removing solid capacity, Scavenging activity are up to 99.2%.
4, the measurement of endocrine anti-aging biological agent scavenging ability of DPPH free radical is adjusted
2.0mL DPPH solution (60 μm of ol/L, absolute ethyl alcohol are solvent) is taken, is added in the adjusting of 1mg embodiments 1 and divides
The anti-aging biological agent secreted places 30min, surveys absorbance value A under 517nm, compareed with distilled water, absorbance at room temperature
Value is AS, the mixed liquor of 3mL water and 2mL absolute ethyl alcohols is blank demodulating apparatus zero, absorbance value A0。
Clearance rate (%)=[A0-(A-AS)]/A0× 100%;
Wherein A is sample liquid light absorption value, A0For blank absorbency, ASTo compare light absorption value.
After measured, embodiment 1 is obtained adjusts endocrine anti-aging biological agent to DPPH free radicals with certain
Scavenging activity, scavenging effect more by force be up to 99.5%.
It can be seen that the inoxidizability for the endocrine anti-aging biological agent of adjusting that the present invention prepares is strong, it can be very
Good adjusting endocrine slows down aging.
Next pair of application of the endocrine anti-aging biological agent of adjusting produced with reference to embodiment 1-3 in clinic
The effect of the present invention is described further.
1, subjects
Female incretion patient 180 of the age at 20-55 Sui is chosen, average age is 31.8 years old, and sick time exists
3.7 years average between 1-5, patient clinical symptom is:The intensely dark pool of skin, loose, pore is coarse, there are a large amount of colors in face
Spot;It is menstruation irregularis, dysmenorrhoea, in poor health, heavy, it is liable to illness.
2, instructions of taking
Above-mentioned patient is equally divided into three groups, there was no significant difference between each group, and three groups of patients take embodiment respectively
The endocrine anti-aging biological agent of adjusting that 1-3 is prepared, daily early, evening take, and each taking 5g, even served 21, observe
And record result.
3, criterion of therapeutical effect
Significantly:Each clinical symptoms such as color spot, irregular menstruation are completely disappeared or are disappeared substantially, and endocrine restores normal;
Effectively:Each clinical symptoms such as color spot, irregular menstruation take an evident turn for the better, and endocrine disorder makes moderate progress;
In vain:Endocrine disorder, each clinical symptoms are without improvement.
4, test result
Most patients are having apparent feeling for 1 week or so according to the method for the present invention using biological agent, and pigment is heavy
Lighter, a small number of spots disappear or become smaller, and skin color improves, and physical condition improves.After drug of the present invention 3 weeks
Effect is shown in Table 1.
1 clinical test results of table
| Number of cases | Effective number | Efficiently individual quantity | Invalid number | Total effective rate | |
| Embodiment 1 | 60 | 42 | 16 | 2 | 96.7% |
| Embodiment 2 | 60 | 38 | 21 | 1 | 98.3% |
| Embodiment 3 | 60 | 45 | 14 | 1 | 98.3% |
As it can be seen from table 1 the effective percentage of the symptoms such as biological agent endocrine disorder provided by the invention reach 96% with
On, it can be seen that, the present invention improves internal system using the synergistic effect of all medicines, by disperse blood stasis and dredge collateral, improves cycle, from
And realize and eliminate siltation in vivo, adjustment incretion balance, make human body functional recovery to good physiological status.In addition, the present invention
Biological agent can also anti-ageing ecchymose removing, improve that skin is yellow, black, relaxed state, desalinate melanin, the supplement a variety of nutrients of skin
Matter desalinates wrinkle.
It should be noted that involved in description of the invention when numberical range, it is thus understood that two of each numberical range
Any one numerical value can be selected between endpoint and two endpoints, since the step method of use is identical as embodiment 1-3, be
It prevents from repeating, description of the invention preferred embodiment is created once a person skilled in the art knows basic
Property concept, then additional changes and modifications may be made to these embodiments.So it includes excellent that the following claims are intended to be interpreted as
It selects embodiment and falls into all change and modification of the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
God and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (10)
1. a kind of adjusting endocrine anti-aging biological agent, which is characterized in that it prepares group of the raw material by following parts by weight
It is grouped as:30-50 parts of grape, 20-30 parts of granatum, 10-15 parts of Radix Astragali, 5-10 parts of Radix Angelicae Sinensis, Jiangnan is Hu 10-20 parts profound, white flower
5-10 parts of mucuna.
2. according to claim 1 adjust endocrine anti-aging biological agent, which is characterized in that its prepare raw material by with
The group of lower parts by weight is grouped as:40 parts of grape, 25 parts of granatum, 10 parts of Radix Astragali, 10 parts of Radix Angelicae Sinensis, 15 parts of the profound Hu in Jiangnan, white flower
5 parts of mucuna.
3. the preparation method according to claim 1 for adjusting endocrine anti-aging biological agent, which is characterized in that specific
Implement according to the following steps:
Step 1,30-50 parts of grape, 20-30 parts of granatum, 10-15 parts of Radix Astragali, 5-10 parts of Radix Angelicae Sinensis, Jiangnan are weighed in parts by weight
Profound Hu 10-20 parts, 5-10 parts of Mucuna birdwoodiana, it is spare;
Step 2, the fruit pulp that grape is weighed in step 1 is detached with grape pip, respectively obtains grape pomace pulp and grape
Seed;
The mashing of grape pomace pulp is broken, obtain grape slurries;
It pulverizes after grape pip is dried, obtains grape pip powder;
Step 3, the water for being equivalent to 10 times of granatum weight is added into the granatum that step 1 weighs, is smashed into pieces slurry, obtained
To pomegranate latex, the pH for adjusting pomegranate latex is 5.0-6.0, and is heated to 45-50 DEG C, is then charged with and is equivalent to pomegranate
The complex enzyme of latex weight 0.1% digests 5h, obtains enzymolysis material;
The grape slurries obtained in step 2 are uniformly mixed with enzymolysis material, mixed fermentation raw material are obtained, into mixed fermentation raw material
The S. cervisiae bacterium solution for being equivalent to mixed fermentation raw material weight 1% is added, seals after stirring evenly, ferments at 30-35 DEG C
15 days, fermentation finished to obtain tunning, and tunning is filtered, ferment filtrate is obtained;
Step 4, it is mixed after the Radix Astragali weighed in step 1, Radix Angelicae Sinensis, the profound Hu in Jiangnan and Mucuna birdwoodiana being crushed, so
It is charged with the alcohol solution dipping for being equivalent to Radix Astragali, 5 times of Radix Angelicae Sinensis, the profound Hu in Jiangnan and Mucuna birdwoodiana total weight amounts afterwards
2h, reheats refluxing extraction 3 times, extracts 2h every time, merges all extracting solutions, filters, and filtrate concentrates at 80 DEG C removes ethyl alcohol,
Obtain Chinese medicine extract;
Step 5, the grape pip powder in step 2, the ferment filtrate in step 3 and the Chinese medicine extract in step 4 are mixed equal
Even, it is 1.2-1.3 to be then concentrated under reduced pressure into density, obtains medicinal extract;By medicinal extract 60 DEG C, vacuum degree be -0.08Mpa under conditions of
Vacuum drying, obtains dry extract, and the dry extract is to adjust endocrine anti-aging biological agent.
4. the preparation method according to claim 3 for adjusting endocrine anti-aging biological agent, which is characterized in that by institute
It states dry extract and pharmaceutically acceptable pharmaceutical preparation is conventionally made.
5. the preparation method according to claim 4 for adjusting endocrine anti-aging biological agent, which is characterized in that described
The pharmaceutical dosage form of pharmaceutical preparation is tablet, capsule, granule, Disket or pill.
6. the preparation method according to claim 3 for adjusting endocrine anti-aging biological agent, which is characterized in that described
The granularity of grape pip powder is 20 mesh in step 2.
7. the preparation method according to claim 3 for adjusting endocrine anti-aging biological agent, which is characterized in that described
Complex enzyme is by pectase, cellulase and carbohydrase according to 2:2:1 quality is formed than mixed preparing.
8. the preparation method according to claim 7 for adjusting endocrine anti-aging biological agent, which is characterized in that described
Pectinase activity, the cellulase activity, the saccharifying enzymic activity are 10000U/mL.
9. the preparation method according to claim 3 for adjusting endocrine anti-aging biological agent, which is characterized in that described
Living bacteria count is 10 in S. cervisiae bacterium solution8-1010cfu/mL。
10. a kind of endocrine anti-aging biological agent of adjusting described in claim 1 is preparing adjusting endocrine, Kangshuaining mixture
Application in object.
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Application publication date: 20181023 |