CN108685938A - The hypoglycemic purposes of beans taro leaf polyose - Google Patents

The hypoglycemic purposes of beans taro leaf polyose Download PDF

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Publication number
CN108685938A
CN108685938A CN201810404020.3A CN201810404020A CN108685938A CN 108685938 A CN108685938 A CN 108685938A CN 201810404020 A CN201810404020 A CN 201810404020A CN 108685938 A CN108685938 A CN 108685938A
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beans taro
taro leaf
leaf polyose
beans
polyose
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郑晓冬
俞露霜
楚强
刘阳阳
李永璐
李禧禧
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Zhejiang University ZJU
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/70Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00Drugs for disorders of the metabolism
    • A61P3/08Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/48Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
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  • General Chemical & Material Sciences (AREA)
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  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
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Abstract

The invention discloses a kind of hypoglycemic purposes of beans taro leaf polyose, and developing natural antihypelipidemic product for fields such as medicine, Food Sciences provides new resources.HepG2 cell models, RINm5F cell model experimental evaluations through high sugar induced structure in the present invention prove, beans taro leaf polyose can significantly improve the ability of HepG2 cell consumptions intake glucose, promote the synthesis of RINm5F cells and excreting insulin, function and effect comprehensively and notable.Therefore, beans taro leaf polyose of the invention can be applied to prepare antihypelipidemic foodstuff or drug, be occurred with treating and preventing diabetes and its chronic diseases.

Description

The hypoglycemic purposes of beans taro leaf polyose
Technical field
The present invention relates to field of medicaments, and in particular to a kind of hypoglycemic purposes of beans taro leaf polyose.
Background technology
U.S. beans taro (Apios americana Medikus) is mainly grown in the Ontario, Canada in North America to the U.S. Between Florida, there is extensive cultivated area in American-European and Japan at present.This is a kind of perennial pulse family Root of Fortune Apios category plant Object is similar to potato.Edible part is underground stem tuber, is a kind of high protein, high-calcium food, thus by American Indian as leading Food.U.S. beans taro introduced China's plantation in 2009, and was cultivated well.Research shows that its flower, leaf, stem tuber are containing rich The ingredients such as rich free amino acid, soluble protein, flavones, isoflavones, saponin(e, vitamin and mineral.Wherein, beans taro contains There are the vitamin, total saposins and isoflavones of high level, there is great potentiality to be exploited.
According to epidemiological analysis, diabetes are a kind of chronic diseases having long history.It can severely impact the mankind Eubolism, such as carbohydrate, lipid and protein metabolism.This metabolic disorder be typically by insulin secretion not Caused by foot or insulin inactivation.Common symptom has:Diuresis, more drinks, more food, weight loss etc., can threat to life when serious. 2000 annual datas statistics shows the torment that the whole world has 1.91 hundred million populations enduring diabetes, it is contemplated that the year two thousand thirty, this number will Reach 3.06 hundred million.Although this is that a kind of coverage is wide, the quite deep disease of harm, since it is practised by heredity, diet, life The used influence for waiting many factors, we are difficult to start in terms of single, find the not high precautionary measures of effective, convenient and cost Therapeutic scheme.How prevention and treatment diabetes and its related complication have caused the concern of whole world scholar, natural plants to be lived Property substance is particularly subject to favor, but the function of polysaccharide of beans taro leaf polyose is rarely reported at present.
Invention content
The technical problem to be solved in the present invention is to provide a kind of hypoglycemic purposes of beans taro leaf polyose, are medicine, Food Science Equal fields develop natural antihypelipidemic product and provide new resources.
In order to solve the above technical problems, the present invention provides a kind of hypoglycemic purposes of beans taro leaf polyose:
It is used to prepare antihypelipidemic foodstuff and/or drug.
The improvement of hypoglycemic purposes as beans taro leaf polyose of the present invention:
It is used to prepare the antihypelipidemic foodstuff and/or drug for improving high sugar induced HepG2 cells to glucose consumption ability.
Hypoglycemic purposes as beans taro leaf polyose of the present invention is further improved:
It is used to prepare the antihypelipidemic foodstuff and/or drug for improving high sugar induced HepG2 cells to glucose uptake ability.
Hypoglycemic purposes as beans taro leaf polyose of the present invention is further improved:
It is used to prepare the antihypelipidemic foodstuff and/or drug for improving high sugar induced RINm5F cells synthesis excreting insulin ability.
Note:Above-mentioned beans taro leaf polyose is obtained by prior art preparation, such as can be prepared by following steps:
1), the preparation of beans taro leaf polyose crude product:
According to 1g:The solid-liquid ratio of 10ml weighs beans taro leaf 100g and 1000mL distilled water is added, and 80 DEG C of water-bath 4h are extracted (enzyme deactivation is also achieved while extraction), is then beaten, then centrifuges (4000r/min centrifuges 30min), by the supernatant of centrifugation gained Liquid filters and collects filtrate;
It is substituted after beans taro leaf repeats above-mentioned addition distilled water with the filter residue of centrifugation gained and first carries out extraction process (80 DEG C of water-baths 4h) and then mashing, the process that centrifuges again 2 times, merge 3 centrifugations and filter the filtrate concentrated by rotary evaporations of gained to the 1/5 of original volume, Obtain concentrate.
Under stirring condition, ethyl alcohol (straight alcohol) is added in the concentrate of gained to ethyl alcohol final concentration of 85%, at 4 DEG C It stands and collects precipitation afterwards for 24 hours, obtain beans taro leaf Thick many candies;
2), the purifying of beans taro leaf Thick many candies, follows the steps below successively:
A, activated carbon decolorizing:
By beans taro leaf Thick many candies be placed in beaker with distillation water dissolution (dosage of distilled water need to only can guarantee that beans taro leaf is slightly more Sugar dissolving), beans taro leaf Thick many candies solution is obtained, activated carbon is added in beans taro leaf Thick many candies solution in 80 ± 10 DEG C of water-baths It is filtered after middle stirring 45min;The mass ratio of beans taro leaf Thick many candies and activated carbon is 15:1;
It is substituted after above-mentioned beans taro leaf Thick many candies solution repeats above-mentioned addition activated carbon and stirring and is filtered with the filtrate of gained Decolorization 3 times, the filtrate of final gained is in colourless, polysaccharide crude solution after must decolourizing at this time;
B, Sevage methods take off albumen:
According to 4:100mL Sevage reagents, Yu Ci is added in polysaccharide crude solution after 400mL decolorations in 1 volume ratio 1000 turns/min rotating speeds stir 30min in power blender, are then centrifuged for, and water layer and position positioned at lower layer are removed by separatory funnel Albuminate between the solvent layer on upper layer;The Sevage reagents are by n-butanol:Chloroform=1:5 volume ratio mixing and ;
Polysaccharide crude solution repeats above-mentioned addition after substituting above-mentioned decoloration with the gains after the removing albuminate of gained Sevage reagents, magnetic agitation, centrifugation remove the de- protein process 5 times of albuminate, must remove the polysaccharide solution of protein;
The polysaccharide solution concentrated by rotary evaporation for removing protein is obtained into concentrate (about the 10% of original volume), by concentrate In -80 DEG C of pre-freeze 6h, then use vacuum freeze drier 48h (technological parameter that vacuum freeze drier is set as -40 DEG C, It 1.2Pa) is dried into powdered (moisture content≤0.1%), obtains beans taro leaf polyose.
The invention discloses a kind of hypoglycemic purposes of beans taro leaf polyose, are the natural drop of the fields such as medicine, Food Science exploitation Sugar product provides new resources.HepG2 cell models, RINm5F cell model experimental evaluations through high sugar induced structure in the present invention It proves, beans taro leaf polyose can significantly improve the ability of HepG2 cellular uptake consumption of glucose, promote the synthesis of RINm5F cells simultaneously Excreting insulin, function and effect are comprehensively and notable.Therefore, beans taro leaf polyose of the invention be applied to prepare antihypelipidemic foodstuff and/or Drug is occurred with treating and preventing diabetes and its chronic diseases.
The usage of the beans taro leaf polyose of the present invention is oral, dosage about 150~250mg every time, three times a day.
The present invention compared with the existing technology, has the following advantages:
1. present invention firstly discovers that beans taro leaf polyose can promote high sugar induced HepG2 cellular uptake consumption of glucose, table Reveal significant hypoglycemic effect, there is good development prospect.
2. present invention firstly discovers that beans taro leaf polyose can promote high sugar induced RINm5F cells to synthesize excreting insulin, tool There is good development prospect.
3. the present invention provides new medical application for beans taro leaf polyose, a new application field has been expanded.
Description of the drawings
Fig. 1 is influence of the various concentration beans taro leaf polyose to HepG2 cell Proliferations in the present invention.
Fig. 2 is influence of the various concentration beans taro leaf polyose to HepG2 cell consumption glucose abilities in the present invention.
Fig. 3 is that (fluorescence shines for influence of the various concentration beans taro leaf polyose to HepG2 cellular uptake glucose abilities in the present invention Piece).
Fig. 4 is that (fluorescence is fixed for influence of the various concentration beans taro leaf polyose to HepG2 cellular uptake glucose abilities in the present invention Amount).
Fig. 5 is influence of the various concentration beans taro leaf polyose to RINm5F cell Proliferations in the present invention.
Fig. 6 is influence of the various concentration beans taro leaf polyose to RINm5F insulin inside cells contents in the present invention.
Fig. 7 is influence of the various concentration beans taro leaf polyose to the extracellular insulin contents of RINm5F in the present invention.
Note:In Fig. 2~Fig. 4 of the present invention:
C represents low sugar control group (DMEM concentration of glucose 5.5mM);
H represents high sugared control group (DMEM concentration of glucose 30mM);
M represents positive controls (DMEM concentration of glucose 30mM+2mM melbine)
In Fig. 6~Fig. 7 of the present invention:
C represents low sugar control group (RPMI-1640 concentration of glucose 4.5mM);
H represents high sugared control group (RPMI-1640 concentration of glucose 16.7mM).
Specific implementation mode
With reference to specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in This:
The hypoglycemic purposes of embodiment 1, beans taro leaf polyose is intervened with beans taro leaf polyose by high sugar as Figure 1-Figure 4 The glucose uptake of the HepG2 cells of induction, observation HepG2 cells consumes ability, illustrates that beans taro leaf polyose has significant drop Sugared effect.
Experiment 1, influence of the beans taro leaf polyose to HepG2 cell Proliferation vigor;
The HepG2 cell inoculations for collecting logarithmic phase are placed in 37 DEG C, 5%CO in 96 orifice plates per 5000, hole cell2Culture It is incubated in case and is grouped afterwards for 24 hours.
It is divided into following 9 groups:Blank control group and 5,10,20,40,80,160,320 and 640ug/mL beans taro leaf polyoses Group;Above-mentioned 5,10,20,40,80,160,320 and 640ug/mL beans taro leaf polyose groups are separately added into the ethyl alcohol extraction of corresponding concentration Object is added without ethanol extract in blank control group.
Every group of 6 hole of repetition, is placed in 37 DEG C, 5%CO2After being incubated for 24 hours jointly in incubator, residual liquid in each hole is sucked out, The MTT (0.5mg/mL is dissolved in not serum-containing media) of 100ul is added afterwards twice with the rinsing of phosphate buffer (PBS) solution, In 37 DEG C, 5%CO2Continue culture 4 hours in incubator.
Then, it removes per boreliquid, 250ul dimethyl sulfoxide (DMSO)s (DMSO) is added per hole, 10 points are shaken on horizontal shaker Clock, microplate reader measure its light absorption value at 570nm.Cell-proliferation activity (%)=experimental port OD values/control wells OD values * 100%; According to Fig. 1, MTT experiment is not the results show that light absorption value has significant difference between each group, illustrate beans taro leaf polyose 0~ It is non-toxic to HepG2 cells in 640ug/mL concentration ranges, the proliferation of HepG2 cells had both been pressed down without facilitation effect or not Effect processed.
Experiment 2, influence of the beans taro leaf polyose to HepG2 cell consumption glucose abilities;
The HepG2 cell inoculations of logarithmic phase are collected in 6cm culture dishes (density 4*105A cell/ware), low sugar training is added Base (DMEM concentration of glucose 5.5mM) is supported, 37 DEG C, 5%CO are placed in2It is grouped after being incubated overnight in incubator;It is equally divided into following 5 Group:Low sugar control group, high sugared control group, melbine positive controls (a concentration of 2mM) and 100ug/mL beans taro leaf polyoses Group and 300ug/mL beans taro leaf polyose groups, each group repeat 5 wares.
Wherein melbine positive controls are added melbine and are pre-processed for 24 hours in incubator.100ug/ml beans taro leaves Polysaccharide group and 300ug/ml beans taro leaf polyose groups are separately added into corresponding concentration beans taro leaf polyose and are pre-processed for 24 hours in incubator.
Replace culture medium:It discards original culture solution and is cleaned twice with PBS, wherein low sugar control group adds low sugar culture medium (DMEM concentration of glucose 5.5mM), remaining group increase sugar culture-medium (DMEM concentration of glucose 30mM).Keep diformazan double simultaneously Determination of metformin and consistent, 100ug/mL beans taro leaf polyose group and 300ug/mL beans taro leaves when pretreatment in guanidine positive controls In polysaccharide group, beans taro leaf polyose concentration is consistent when being pre-processed with it.
By above-mentioned each group in incubator coprocessing for 24 hours after, replace culture medium:Discard original culture solution in culture dish It is used in combination PBS to clean twice, changes low sugar culture medium into later.12h is cultivated in incubator.
Cell conditioned medium is collected, the glucose utilization in culture solution is measured using glucose kit.Discard extra training Nutrient solution is simultaneously cleaned twice with PBS, and 1ml cell pyrolysis liquids are added in each culture dish, 2ml centrifuge tubes are collected in cell scraper.Ultrasound Afterwards quantitative and corrected glucose consumption data are carried out with BCA protein reagents box.
According to Fig.2, glucose consumption experimental result is shown, the beans taro leaf polyose of 100ug/ml and 300ug/ml concentration It can obviously repair by the glucose absorption ability of the lower impaired HepG2 cells of high sugar effect, and 100ug/ml and 300ug/ml The beans taro leaf polyose effect of concentration is similar.
Experiment 3, influence of the beans taro leaf polyose to HepG2 cellular uptake glucose abilities;
The HepG2 cell inoculations of collection logarithmic phase (density 4*10 in 24 orifice plates5A cells/well), low sugar training is added Support base (DMEM concentration of glucose 5.5mM), be placed in 37 DEG C, be incubated overnight in 5%CO2 incubators after be grouped;It is respectively divided into following Five groups:Low sugar control group, high sugared control group, melbine positive controls (a concentration of 2mM), 100ug/mL beans taro leaf polyose groups With 300ug/mL beans taro leaf polyose groups, each group repeats 3 holes.
Wherein melbine positive controls are added melbine and are pre-processed for 24 hours in incubator.100ug/ml beans taro leaves Polysaccharide group and 300ug/ml beans taro leaf polyose groups are separately added into corresponding concentration beans taro leaf polyose and are pre-processed for 24 hours in incubator.
Replace culture medium:It discards original culture solution and is cleaned twice with PBS, wherein low sugar control group adds low sugar culture medium (DMEM concentration of glucose 5.5mM), remaining group increase sugar culture-medium (DMEM concentration of glucose 30mM).Keep diformazan double simultaneously Determination of metformin and consistent, 100ug/mL beans taro leaf polyose group and 300ug/mL beans taro leaves when pretreatment in guanidine positive controls It is consistent when beans taro leaf polyose concentration is pre-processed with it in polysaccharide group.
In incubator coprocessing for 24 hours after, discard culture medium and cleaned twice with PBS, change into 2-NBDG containing 0.1mM spy The fresh culture of needle and 100nM insulin, cultivates 30min in incubator.After dyestuff is clean simultaneously with fluorescence microscope It takes pictures, calculates average optical density value.
According to fig. 3 and shown in Fig. 4, glucose uptake experimental result is shown, compared to low sugar condition, under high sugar effect, HepG2 cells can absorb more glucose, but gap unobvious.Beans taro leaf polyose can improve HepG2 cellular uptakes Portugal The ability of grape sugar.Wherein, compared to high sugared control group, the function and effect of the beans taro leaf polyose (300ug/ml) of high concentration are very bright It is aobvious.
Embodiment 2, as shown in Figure 5-Figure 7, intervened the RINm5F cells by high sugar induced with beans taro leaf polyose, observe The insulin synthesis of RINm5F cells secretes situation, illustrates that beans taro leaf polyose has significant hypoglycemic effect.
Experiment 1, influence of the beans taro leaf polyose to RINm5F cell Proliferation vigor;
The RINm5F cell inoculations of logarithmic phase are collected in 96 orifice plates, per 5000, hole cell, are placed in 37 DEG C, 5%CO2 cultures It is incubated in case and is grouped afterwards for 24 hours.
It is divided into following 9 groups:Blank control group and 5,10,20,40,80,160,320 and 640ug/mL beans taro leaf polyoses Group;Above-mentioned 5,10,20,40,80,160,320 and 640ug/mL beans taro leaf polyose groups are separately added into the ethyl alcohol extraction of corresponding concentration Object is added without ethanol extract in blank control group.
Every group of 6 hole of repetition, is placed in 37 DEG C, 5%CO2After being incubated for 24 hours jointly in incubator, residual liquid in each hole is sucked out, The MTT (0.5mg/mL is dissolved in not serum-containing media) of 100ul is added afterwards twice with the rinsing of phosphate buffer (PBS) solution, In 37 DEG C, 5%CO2Continue culture 4 hours in incubator.
Then, it removes per boreliquid, 250ul dimethyl sulfoxide (DMSO)s (DMSO) is added per hole, 10 points are shaken on horizontal shaker Clock, microplate reader measure its light absorption value at 570nm.Cell-proliferation activity (%)=experimental port OD values/control wells OD values * 100%; According to Fig.5, MTT experiment is the results show that compared with blank control group, and the beans taro leaf polyose of 40~640ug/ml concentration is to thin Born of the same parents' proliferation activity has apparent facilitation.Beans taro leaf polyose is nontoxic to RINm5F cells in 0-640ug/mL concentration ranges Property.
Experiment 2, beans taro leaf polyose synthesize RINm5F cells the influence of excreting insulin;
The RINm5F cell inoculations of logarithmic phase are collected in 6cm culture dishes (density 4*105A cell/ware), low sugar is added Culture medium (RPMI-1640 concentration of glucose 4.5mM), be placed in 37 DEG C, be incubated overnight in 5%CO2 incubators after be grouped, that is, point For low sugar control group, high sugared control group, 200ug/mL beans taro leaf polyose group and 300ug/mL beans taro leaf polyose groups.Wherein 200ug/ The beans taro leaf polyose that corresponding concentration is added in mL beans taro leaf polyose group and 300ug/ml beans taro leaf polyose groups pre-processes in incubator 24h。
Replace culture medium:It discards original culture solution in hole and is cleaned twice with PBS, wherein low sugar control group adds low sugar to train Base (RPMI-1640 concentration of glucose 4.5mM) is supported, remaining increases sugar culture-medium (RPMI-1640 concentration of glucose 16.7mM) simultaneously The beans taro leaf polyose concentration for keeping beans taro leaf polyose group consistent with when pretreatment.In incubator coprocessing for 24 hours after, replace culture Base:It discards original culture solution in culture dish and is cleaned twice with PBS, change low sugar culture medium into without exception.It is cultivated in incubator 12h。
Cell conditioned medium is collected, the insulin content in Elisa kit measurement culture solutions is used.Discard extra culture solution It is used in combination PBS to clean twice, 1mlPBS is added in each ware, 2ml centrifuge tubes are collected in cell scraper.A part of use is taken after ultrasonication BCA protein reagent boxes are quantified, and Elisa kit measurement insulin contents are used after rest part centrifugation.By intraor extracellular Insulin content is corrected with albumen concentration.
According to Fig. 6,7, the results show that under high sugar effect, the synthesis of RINm5F cells divides insulin content determination experiment The ability for secreting insulin is impaired.200,300 and 400ug/ml beans taros leaf polyose can obviously be repaired lower impaired by high sugar effect RINm5F cells synthesis and excreting insulin ability, significant effect.Show that beans taro leaf polyose can promote islet cells to increase Excreting insulin is grown and synthesizes, it is great for the therapeutic potential of hypoinsulinism class patients with type Ⅰ DM.
The present invention proposes a kind of hypoglycemic purposes of beans taro leaf polyose, is that natural hypoglycemic is developed in the fields such as medicine, Food Science Product provides new resources.It is related to application of the beans taro leaf polyose in preparing antihypelipidemic product simultaneously.That listed above is only the present invention Implementation several examples.It is clear that the invention is not restricted to above example, acceptable there are many deformations.The common skill of this field All deformations that art personnel directly can export or associate from present disclosure are considered as the protection model of the present invention It encloses.

Claims (4)

1. the hypoglycemic purposes of beans taro leaf polyose, it is characterised in that:
It is used to prepare antihypelipidemic foodstuff and/or drug.
2. the hypoglycemic purposes of beans taro leaf polyose according to claim 1, it is characterised in that:
It is used to prepare the antihypelipidemic foodstuff and/or drug for improving high sugar induced HepG2 cells to glucose consumption ability.
3. the hypoglycemic purposes of beans taro leaf polyose according to claim 1, it is characterised in that:
It is used to prepare the antihypelipidemic foodstuff and/or drug for improving high sugar induced HepG2 cells to glucose uptake ability.
4. the hypoglycemic purposes of beans taro leaf polyose according to claim 1, it is characterised in that:
It is used to prepare the antihypelipidemic foodstuff and/or drug for improving high sugar induced RINm5F cells synthesis excreting insulin ability.
CN201810404020.3A 2018-04-28 2018-04-28 The hypoglycemic purposes of beans taro leaf polyose Pending CN108685938A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109512812A (en) * 2018-12-14 2019-03-26 江苏省农业科学院 A kind of glucose induction human liver cancer cell HepG2 establishes the method and application of diabetes model

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
安家炜等: "美国豆芋地上部位有效成分及α- 葡萄糖苷酶抑制活性研究", 《核农学报》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109512812A (en) * 2018-12-14 2019-03-26 江苏省农业科学院 A kind of glucose induction human liver cancer cell HepG2 establishes the method and application of diabetes model

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