CN108676850A - A kind of exercise metabolism gene detecting kit - Google Patents
A kind of exercise metabolism gene detecting kit Download PDFInfo
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- CN108676850A CN108676850A CN201810598687.1A CN201810598687A CN108676850A CN 108676850 A CN108676850 A CN 108676850A CN 201810598687 A CN201810598687 A CN 201810598687A CN 108676850 A CN108676850 A CN 108676850A
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- probe
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- exercise metabolism
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Abstract
The present invention discloses a kind of exercise metabolism gene detecting kit, which includes exercise metabolism genetic chip, and the exercise metabolism genetic chip includes probe groups A and multiple PCR primer group 1；The multiple PCR primer group 1 includes primer 1, primer 2, primer 3, primer 4, primer 5, primer 6, primer 7, primer 8, primer 9 and primer 10；The probe groups A includes probe 1, probe 2, probe 3, probe 4, probe 5, probe 6, probe 7, probe 8, probe 9 and probe 10；Each probe is each attached on solid phase carrier.The kit is detected using exercise metabolism genetic chip, can be quickly detected to the metabolic capability of nutriment to individual, in conjunction with questionnaire and actual observation, testing result sensitivity is good, and accuracy rate is high, highly practical, and sampling is convenient, use easy to spread.
Description
Technical field
The present invention relates to technical field of gene detection, especially a kind of exercise metabolism gene detecting kit.
Background technology
Genetic test refers to being detected to genomic DNA by blood, Oral Mucosal Cells or other histocytes
Technology.After extracting the DNA in cell and expanding related gene, the gene information in cell is examined by particular instrument equipment
It surveys, analyzes the genotype information contained by it, the difference for making people understand itself with other people from gene level is directed to utilize
The influence that related gene is likely to result in is prevented or improved to the means of property.By genetic test, SNP points of individual can be defined
Type understands these SNP sites, so that it may to judge heredity and the health status of the individual.
Basic metabolism is the energy expenditure for maintaining body vital movement most basic, and human body is to food ingredients (such as carbon hydrate
Object, lipid, protein) metabolic condition and gene relationship it is close, the individual of different SNP site Genotypings, to food
The metabolic capability of ingredient difference.
However not yet find a kind of exercise metabolism gene detecting kit at present, it can be quickly to individual to nutriment
The metabolic capability of (such as carbohydrate, lipid, protein) is detected, and knows itself to carbon hydrate in advance conducive to people
The metabolic capability situation of object, lipid and protein, specific aim carry out diet, prevent to lead to nutritional ingredient because of metabolic capability deficiency
Accumulation, so that people is eaten healthier.
Invention content
In order to overcome the disadvantages mentioned above of the prior art, that the object of the present invention is to provide a kind of sensitivity is good, accuracy rate is high, real
Exercise metabolism gene detecting kit strong with property, that quickly individual can be detected the metabolic capability of nutriment.
The technical solution adopted by the present invention to solve the technical problems is：
A kind of exercise metabolism gene detecting kit, the kit include exercise metabolism genetic chip, the exercise metabolism
Genetic chip includes probe groups A and multiple PCR primer group 1；
C.46-23525T the probe groups A is used for gene containing FTO>The sites A, TCF7L2 genes are c.552+9017G
>The sites T, APOA5 genes c.-620C>The sites T, LDLR genes c.*52G>The c.-901G in the sites A and CTSS genes>A
The nucleic acid fragment of point carries out specific amplification；
C.46-23525T the multiple PCR primer group 1 is used for FTO genes>A loci gene types, TCF7L2 genes
c.552+9017G>The c.-620C of T loci gene types, APOA5 genes>The c.*52G of T loci gene types, LDLR genes>The sites A
The c.-901G of genotype and CTSS genes>A loci gene types carry out specific detection.
As a further improvement on the present invention：The multiple PCR primer group 1 include primer 1, primer 2, primer 3, primer 4,
Primer 5, primer 6, primer 7, primer 8, primer 9 and primer 10, the sequence of above-mentioned primer are as follows：
(1) primer 1：5’-CACTAACATCAGTTATGCAT-3’；
(2) primer 2：5’-CTCTCCCACTCCATTTCTGC-3’；
(3) primer 3：5’-GTCCAGTTTACACATAAGGA-3’；
(4) primer 4：5’-ATCTGGCACTCAGAAGAGAG-3’；
(5) primer 5：5’-CAGGGTGAAGATGAGATGGC-3’；
(6) primer 6：5’-CTGAGCATTTGGGCTTGCTC-3’；
(7) primer 7：5’-GTGGCGTGAACATCTGCCTG-3’；
(8) primer 8：5’-GATGAATAAATATATAAAAC-3’；
(9) primer 9：5’-CTCAAATATATCCAATTGAG-3’；
(10) primer 10：5-TGAAAAGATCCTCAACCTCA-3’.
As a further improvement on the present invention：The probe groups A include probe 1, probe 2, probe 3, probe 4, probe 5,
Probe 6, probe 7, probe 8, probe 9 and probe 10, the sequence of above-mentioned probe are as follows：
(1) probe 1：5’-TGTGAATTTTGTGATGCAC-3’；
(2) probe 2：5’-TGTGAATTTAGTGATGCAC-3’；
(3) probe 3：5’-GGCAAGAATGACCATATTC-3’；
(4) probe 4：5’-GGCAAGAATTACCATATTC-3’；
(5) probe 5：5’-AGCGAAAGTGAGATTTGCC-3’；
(6) probe 6：5’-AGCGAAAGTAAGATTTGCC-3’；
(7) probe 7：5’-GACCTCGCCGGCCTTGTTT-3’；
(8) probe 8：5’-GACCTCGCCAGCCTTGTTT-3’；
(9) probe 9：5’-GGAGCAAGACGAACTATCA-3’；
(10) probe 10：5-GGAGCAAGATGAACTATCA-3’.
As a further improvement on the present invention：The probe groups A includes a variety of probes, each probe is each attached to solid phase
On carrier.
As a further improvement on the present invention：The solid phase carrier is in nitrocellulose filter, nylon membrane, polystyrene
It is at least one.
As a further improvement on the present invention：The kit further includes hybridization solution and eluent.
As a further improvement on the present invention：The hybridization solution includes the formamide and matter that mass concentration percentage is 50%
Measure the lauryl sodium sulfate that percentage is 0.2%.
As a further improvement on the present invention：The eluent is absolute ethyl alcohol.
Compared with prior art, the beneficial effects of the invention are as follows：
The kit of the present invention can quickly be detected the metabolic capability of nutriment individual, shift to an earlier date conducive to people
Know that itself metabolic capability situation to carbohydrate, lipid and protein, specific aim carry out diet, prevents because of metabolic capability
Accumulation that is insufficient and leading to nutritional ingredient, makes people eat healthier.The kit of the present invention uses exercise metabolism genetic chip
It is detected, in conjunction with questionnaire and actual observation, testing result sensitivity is good, and accuracy rate is high, highly practical, and sampling side
Just, use easy to spread.
Specific implementation mode
In conjunction with embodiment, the present invention is further described：
A kind of exercise metabolism gene detecting kit, the kit include exercise metabolism genetic chip, the exercise metabolism
Genetic chip includes probe groups A and multiple PCR primer group 1；
C.46-23525T the probe groups A is used for gene containing FTO>The sites A, TCF7L2 genes are c.552+9017G
>The sites T, APOA5 genes c.-620C>The sites T, LDLR genes c.*52G>The c.-901G in the sites A and CTSS genes>A
The nucleic acid fragment of point carries out specific amplification；
C.46-23525T the multiple PCR primer group 1 is used for FTO genes>A loci gene types, TCF7L2 genes
c.552+9017G>The c.-620C of T loci gene types, APOA5 genes>The c.*52G of T loci gene types, LDLR genes>The sites A
The c.-901G of genotype and CTSS genes>A loci gene types carry out specific detection.
The multiple PCR primer group 1 includes primer 1, primer 2, primer 3, primer 4, primer 5, primer 6, primer 7, primer
8, primer 9 and primer 10, the sequence of above-mentioned primer are as follows：
(1) primer 1：5’-CACTAACATCAGTTATGCAT-3’；
(2) primer 2：5’-CTCTCCCACTCCATTTCTGC-3’；
(3) primer 3：5’-GTCCAGTTTACACATAAGGA-3’；
(4) primer 4：5’-ATCTGGCACTCAGAAGAGAG-3’；
(5) primer 5：5’-CAGGGTGAAGATGAGATGGC-3’；
(6) primer 6：5’-CTGAGCATTTGGGCTTGCTC-3’；
(7) primer 7：5’-GTGGCGTGAACATCTGCCTG-3’；
(8) primer 8：5’-GATGAATAAATATATAAAAC-3’；
(9) primer 9：5’-CTCAAATATATCCAATTGAG-3’；
(10) primer 10：5-TGAAAAGATCCTCAACCTCA-3’.
The probe groups A includes probe 1, probe 2, probe 3, probe 4, probe 5, probe 6, probe 7, probe 8, probe 9
It is as follows with the sequence of probe 10, above-mentioned probe：
(1) probe 1：5’-TGTGAATTTTGTGATGCAC-3’；
(2) probe 2：5’-TGTGAATTTAGTGATGCAC-3’；
(3) probe 3：5’-GGCAAGAATGACCATATTC-3’；
(4) probe 4：5’-GGCAAGAATTACCATATTC-3’；
(5) probe 5：5’-AGCGAAAGTGAGATTTGCC-3’；
(6) probe 6：5’-AGCGAAAGTAAGATTTGCC-3’；
(7) probe 7：5’-GACCTCGCCGGCCTTGTTT-3’；
(8) probe 8：5’-GACCTCGCCAGCCTTGTTT-3’；
(9) probe 9：5’-GGAGCAAGACGAACTATCA-3’；
(10) probe 10：5-GGAGCAAGATGAACTATCA-3’.
The probe groups A includes a variety of probes, each probe is each attached on solid phase carrier.The solid phase carrier is nitre
At least one of acid cellulose film, nylon membrane, polystyrene.
The kit further includes hybridization solution and eluent.The hybridization solution includes the formyl that mass concentration percentage is 50%
The lauryl sodium sulfate that amine and mass concentration percentage are 0.2%.The eluent is absolute ethyl alcohol.
Embodiment 1：In multiple subjects, possess FTO genes c.46-23525T>The sites A TT genotype or TCF7L2 bases
Because c.552+9017G>The subject of the sites T GG genotype has stronger carbohydrate metabolism ability, these subjects warp
It crosses actual observation and the excessively high situation of blood glucose is less likely to occur really with questionnaire discovery；Possess FTO genes c.46-23525T>A
Site AT genotype or TCF7L2 genes are c.552+9017G>The subject of the sites T GT genotype has general carbohydrate
Metabolic capability, this kind of subject of actual observation has found, wherein there is groups of people's blood glucose excessively high, is learnt by questionnaire, these by
Inspection person, which is substantially, takes in excessive carbohydrate food；Possess FTO genes c.46-23525T>The sites A AA genotype or
TCF7L2 genes are c.552+9017G>The subject of the sites T TT genotype has weaker carbohydrate metabolism ability, practical
Investigation is learnt, even if this kind of subject takes in seldom carbohydrate and also easily causes blood glucose rise.
Embodiment 2：In multiple subjects, possess APOA5 genes c.-620C>The sites T CC genotype or LDLR genes
c.*52G>The subject of the sites A GG genotype has stronger lipid-metabolism ability, these subjects are by actual observation and tune
Volume is interrogated to find really to be not easy that obesity occurs because of fat accumulation；Possess APOA5 genes c.-620C>The sites T CT genotype or
LDLR genes c.*52G>The subject of the sites A AG genotype has general lipid-metabolism ability, this kind of subject of actual observation
It was found that wherein there is groups of people's heavier-weight, learnt by questionnaire, these subjects are substantially the group foods such as intake frying
More people；Possess APOA5 genes c.-620C>The sites T TT genotype or LDLR genes c.*52G>The sites A AA genotype by
Inspection person has weaker lipid-metabolism ability, and factual survey is learnt, even if this kind of subject eats seldom greasy food and is also easy
Cause fat accumulation and leads to obesity.
Embodiment 3：In multiple subjects, possess CTSS genes c.-901G>The subject of the sites A GG genotype has
Stronger protein metabolism ability, these subjects are not easy by actual observation and questionnaire discovery due to protein loss
Caused Health cost；Possess CTSS genes c.-901G>The subject of the sites A AG genotype has general protein metabolism
Ability, this kind of subject of factual survey have found that wherein some people has the additional experience for supplementing protein；Possess CTSS bases
Because of c.-901G>The subject of the sites A AA genotype has weaker protein metabolism ability, and factual survey is learnt, this kind of to be examined
There is a big chunk people to have the bodies undesirable condition such as malnutrition caused by protein lacks in person.
In conclusion after those skilled in the art read file of the present invention, according to the technique and scheme of the present invention with
Technical concept is not necessarily to creative mental labour and makes other various corresponding conversion schemes, belongs to the model that the present invention is protected
It encloses.
Claims (8)
1. a kind of exercise metabolism gene detecting kit, it is characterised in that：The kit includes exercise metabolism genetic chip, described
Exercise metabolism genetic chip includes probe groups A and multiple PCR primer group 1；
C.46-23525T the probe groups A is used for gene containing FTO>The sites A, TCF7L2 genes are c.552+9017G>T
The c.-620C of point, APOA5 genes>The sites T, LDLR genes c.*52G>The c.-901G in the sites A and CTSS genes>The sites A
Nucleic acid fragment carries out specific amplification；
C.46-23525T the multiple PCR primer group 1 is used for FTO genes>A loci gene types, TCF7L2 genes
c.552+9017G>The c.-620C of T loci gene types, APOA5 genes>The c.*52G of T loci gene types, LDLR genes>The sites A
The c.-901G of genotype and CTSS genes>A loci gene types carry out specific detection.
2. a kind of exercise metabolism gene detecting kit according to claim 1, it is characterised in that：The multiplex PCR draws
Object group 1 includes primer 1, primer 2, primer 3, primer 4, primer 5, primer 6, primer 7, primer 8, primer 9 and primer 10, above-mentioned to draw
The sequence of object is as follows：
(1) primer 1：5’-CACTAACATCAGTTATGCAT-3’；
(2) primer 2：5’-CTCTCCCACTCCATTTCTGC-3’；
(3) primer 3：5’-GTCCAGTTTACACATAAGGA-3’；
(4) primer 4：5’-ATCTGGCACTCAGAAGAGAG-3’；
(5) primer 5：5’-CAGGGTGAAGATGAGATGGC-3’；
(6) primer 6：5’-CTGAGCATTTGGGCTTGCTC-3’；
(7) primer 7：5’-GTGGCGTGAACATCTGCCTG-3’；
(8) primer 8：5’-GATGAATAAATATATAAAAC-3’；
(9) primer 9：5’-CTCAAATATATCCAATTGAG-3’；
(10) primer 10：5-TGAAAAGATCCTCAACCTCA-3’.
3. a kind of exercise metabolism gene detecting kit according to claim 1, it is characterised in that：The probe groups A packets
Include probe 1, probe 2, probe 3, probe 4, probe 5, probe 6, probe 7, probe 8, probe 9 and probe 10, the sequence of above-mentioned probe
Row are as follows：
(1) probe 1：5’-TGTGAATTTTGTGATGCAC-3’；
(2) probe 2：5’-TGTGAATTTAGTGATGCAC-3’；
(3) probe 3：5’-GGCAAGAATGACCATATTC-3’；
(4) probe 4：5’-GGCAAGAATTACCATATTC-3’；
(5) probe 5：5’-AGCGAAAGTGAGATTTGCC-3’；
(6) probe 6：5’-AGCGAAAGTAAGATTTGCC-3’；
(7) probe 7：5’-GACCTCGCCGGCCTTGTTT-3’；
(8) probe 8：5’-GACCTCGCCAGCCTTGTTT-3’；
(9) probe 9：5’-GGAGCAAGACGAACTATCA-3’；
(10) probe 10：5-GGAGCAAGATGAACTATCA-3’.
4. a kind of exercise metabolism gene detecting kit according to claim 1, it is characterised in that：The probe groups A packets
A variety of probes are included, each probe is each attached on solid phase carrier.
5. a kind of exercise metabolism gene detecting kit according to claim 4, it is characterised in that：The solid phase carrier is
At least one of nitrocellulose filter, nylon membrane, polystyrene.
6. a kind of exercise metabolism gene detecting kit according to claim 1, it is characterised in that：The kit further includes
Hybridization solution and eluent.
7. a kind of exercise metabolism gene detecting kit according to claim 6, it is characterised in that：The hybridization solution includes
The lauryl sodium sulfate that the formamide and mass concentration percentage that mass concentration percentage is 50% are 0.2%.
8. a kind of exercise metabolism gene detecting kit according to claim 6, it is characterised in that：The eluent is nothing
Water-ethanol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810598687.1A CN108676850A (en) | 2018-06-12 | 2018-06-12 | A kind of exercise metabolism gene detecting kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810598687.1A CN108676850A (en) | 2018-06-12 | 2018-06-12 | A kind of exercise metabolism gene detecting kit |
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| CN108676850A true CN108676850A (en) | 2018-10-19 |
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|---|---|---|---|
| CN201810598687.1A Pending CN108676850A (en) | 2018-06-12 | 2018-06-12 | A kind of exercise metabolism gene detecting kit |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103180458A (en) * | 2009-10-16 | 2013-06-26 | 基因投资私人有限公司 | Macronutrient sensitivity |
| CN106086222A (en) * | 2016-08-24 | 2016-11-09 | 厦门美因生物科技有限公司 | Motion detecting and evaluating genes method and system based on qPCR typing method |
-
2018
- 2018-06-12 CN CN201810598687.1A patent/CN108676850A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103180458A (en) * | 2009-10-16 | 2013-06-26 | 基因投资私人有限公司 | Macronutrient sensitivity |
| CN106086222A (en) * | 2016-08-24 | 2016-11-09 | 厦门美因生物科技有限公司 | Motion detecting and evaluating genes method and system based on qPCR typing method |
Non-Patent Citations (3)
| Title |
|---|
| HENRI HOOTON等: "Dietary factors impact on the association between CTSS variants and obesity related traits", 《PLOS ONE》 * |
| TERTIA VAN ZYL等: "Common and rare single nucleotide polymorphisms in the LDLR gene are present in a black South African population and associate with low-density lipoprotein cholesterol levels", 《JOURNAL OF HUMAN GENETICS》 * |
| 阿克巴尼亚等等: "《儿童脊柱外科学》", 30 November 2012, 人民军医出版社 * |
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Application publication date: 20181019 |