CN106086222A - Motion detecting and evaluating genes method and system based on qPCR typing method - Google Patents
Motion detecting and evaluating genes method and system based on qPCR typing method Download PDFInfo
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Abstract
The invention provides a kind of motion detecting and evaluating genes method based on qPCR typing method, described gene assessment system carries out fluorescent PCR detection to the motion gene polymorphism sites of person under inspection, and give the contribution degree that different pleomorphism sites is different, according to gene test result and the statistical analysis of different contribution degrees, drawing the assessment result of individuation, assessment result collects foundation motion talent's gene database.The present invention can be estimated all sidedly in motor capacity and relevant risk to person under inspection, the basis of assessment is precisely, assessment efficiency is high and assessment result stablize accurate.During recruiting athlete, existing selection means can be assisted to carry out athlete and to select；During training, athlete can be done individual training and instruct and management, the method that instant invention overcomes the single solution for diverse problems of traditional method, in selection of sportsmen and training, and individual body-building effect aspect more advantage.
Description
Technical field
The present invention relates to field of biological detection, particularly relate to detecting and evaluating genes system, specifically a kind of based on
The motion detecting and evaluating genes method and system of qPCR typing method.
Background technology
Along with the development of scientific research, after the Human Genome Project completes, being in full swing of post genome project, with
The biotechnology that genetic engineering is taken as the leading factor is paid close attention to widely in the application of sports problem domain.Motion gene is to determine
The related gene of mankind and nature, motor capacity 70% is affected gene genetic engineer applied in selection of athletes by inherited genetic factors
Being trend of the times, from molecular level, it can disclose why athlete moves outstanding reason, this technology will be fundamentally
Cause motion selection theoretical and the revolution of method.Genetic theory and method is used at present to carry out at the beginning of motion selection had
Stepping exhibition.Conventional gene tester has: direct Sequencing (direct sequencing, DS), Ligase detection reaction
(ligase detection reaction, LDR), restriction fragment length polymorphism analysis (restriction fragment
Length polymorphism, RFLP), dhplc analysis (denaturing high performance
Liquid chromotography, DHPLC), quantitative PCR, wherein utilizing DNA analysis instrument direct Sequencing is goldstandard, but on
The problems such as the method for stating is respectively present that cost is high, accuracy rate is low, complex operation and poor repeatability.Real time fluorescent quantitative nucleic acid amplification
Detecting system (Real-time Quantitative PCR Detecting System, qPCR), also makes real-time quantitative gene expand
Increasing fluorescence detecting system, qPCR system is the PCR detection technique of three generations, and it is high that qPCR has detection sensitivity, detects the range of linearity
Width, accuracy of detection and the outstanding advantage such as reproducible, be therefore acknowledged as the world today for clinical most advanced nucleic acid molecules
Diagnostic techniques.Therefore this technology can be used for motion gene test efficiently, accurately.
In current prior art, the detection majority of motion gene is unit point effect study, the existing product on market
Being the most only the combination using minority site, different test kits uses the different assortment of genes, and results contrast is unilateral, and to same
The testing result of testee there may be bigger difference.China Patent Publication No. CN 101851674A, CN 104313147A,
These type of documents such as CN 102864223A and CN 104517023A are all the combine detection to componental movement gene, and detect knot
Really standard differs, and different testing results is difficult to unified judge, and versatility is poor.Therefore motion gene can be carried out entirely by one
The detection in face also carries out the problem in the urgent need to address that the assessment system of Scientific evaluation is this area.
Summary of the invention
For this reason, it may be necessary to provide a kind of total movement gene polynorphisms site can be detected based on qPCR typing
The motion detecting and evaluating genes method and system of technology.
For achieving the above object, motion detecting and evaluating genes method and system based on qPCR typing method, to person under inspection
Motion gene polymorphism sites carry out fluorescent PCR detection, genotype is carried out score assessment；According to gene test result and comment
The statistical analysis divided, draws the assessment result of individuation；Assessment result collects foundation motion talent's gene database.
Preferably, motion gene includes following aspect:
Endurance, explosive strength quality, motion enthusiasm, sportive recovery from fatigue, soft tissue injury risk, risk of obesity,
Carbohydrate and lipid sensitivity, 52 pleomorphism sites of 44 related genes of totally 8 aspects.Wherein, endurance bag
Including 13 pleomorphism sites, explosive strength quality includes 11 pleomorphism sites, and motion enthusiasm includes 6 pleomorphism sites, fortune
Dynamic fatigue recovery includes 6 pleomorphism sites, and soft tissue injury risk includes 5 pleomorphism sites, and risk of obesity includes 11
Pleomorphism site, carbohydrate sensitivity includes 6 pleomorphism sites, and lipid sensitivity includes 7 pleomorphism sites.
It is further preferred that the contribution margin of each pleomorphism site is: capability evaluation pleomorphism site has according to genotype
Sharp or blunt and assignment 2 points, favourable heterozygosis assignment 1 point, without affecting assignment 0 point；Risk assessment pleomorphism site is according to genotype risk
Pure and mild assignment 2 points, risk heterozygosis assignment 1 point, without affecting assignment 0 point；
Concrete motion gene loci and contribution margin be:
13 sites of endurance assessment, its different genotype and being entered as: ADRB2 gene rs1042713, AA-AG-
GG genotype is respectively 2-1-0 and divides；AQP1 gene rs1049305, CC-CG-GG genotype is divided into divides for 2-1-0；AMPD1 gene
Rs17602729, CC-CT-TT genotype is respectively 2-1-0 and divides；COL5A1 gene rs12722, TT-CT-CC genotype is respectively
2-1-0 divides；GABPB1 gene rs12594956, TT-GT-GG genotype is respectively 2-1-0 and divides；GABPB1 gene rs7181866,
CC-CT-TT genotype is respectively 2-1-0 and divides；HFE gene rs1799945, GG-CG-CC genotype is respectively 2-1-0 and divides；
KCNJ11 gene rs5219, CC-CT-TT genotype is respectively 2-1-0 and divides；PPARA gene rs4253778, GG-CG-CC gene
Type is respectively 2-1-0 and divides；PPARD gene rs2016520, CC-CT-TT genotype is respectively 2-1-0 and divides；UCP3 gene
Rs1800849, TT-CT-CC genotype is respectively 2-1-0 and divides；ACE gene rs4646994, II-DI-DD genotype is respectively 2-
1-0 divides；PPARGC1A gene rs8192678, GG-AG-AA genotype is respectively 2-1-0 and divides；
11 sites of explosive strength competency assessment, its different genotype and being entered as: AGT gene rs699 site, CC-
CT-TT genotype is respectively 2-1-0 and divides；AMPD1 gene rs17602729, CC-CT-TT genotype is respectively 2-1-0 and divides；CKM
Gene rs8111989, GG-AG-AA genotype is respectively 2-1-0 and divides；HIF1A gene rs11549465, TT-CT-CC genotype
It is respectively 2-1-0 to divide；IL6 gene rs1800795, GG-CG-CC genotype is respectively 2-1-0 and divides；NOS3 gene rs2070744,
TT-CT-CC genotype is respectively 2-1-0 and divides；PPARA gene rs4253778, CC-CG-GG genotype is respectively 2-1-0 and divides；
PPARG gene rs1801282, GG-CG-CC genotype is respectively 2-1-0 and divides；ACE gene rs4646994, DD-DI-II gene
Type is respectively 2-1-0 and divides；ACTN3 gene rs1815739, CC-CT-TT genotype is respectively 2-1-0 and divides；Mthfr gene
Rs1801131, CC-AC-AA genotype is respectively 2-1-0 and divides；
6 sites of motion enthusiasm assessment, its different genotype and being entered as: CREB1 gene rs2253206, GG-AG-
AA genotype is respectively 2-1-0 and divides；C18orf2 gene rs8097348, GG-AG-AA genotype is respectively 2-1-0 and divides；AMPD1
Gene rs17602729, CC-CT-TT genotype is respectively 2-1-0 and divides；PATS2L gene rs12612420, AA-AG-GG gene
Type is respectively 2-1-0 and divides；PAPSS2 gene rs10887741, TT-CT-CC genotype is respectively 2-1-0 and divides；BDNF gene
Rs6265, AA-AG-GG genotype is respectively 2-1-0 and divides；
6 sites of sportive recovery from fatigue assessment, its different genotype and being entered as: AMPD1 gene rs17602729,
CC-CT-TT genotype is respectively 2-1-0 and divides；IL6 gene rs1800795, GG-CG-CC genotype is respectively 2-1-0 and divides；
HIF1A gene rs11549465, TT-CT-CC genotype is respectively 2-1-0 and divides；NAT2 gene rs1208, GG-AG-AA genotype
It is respectively 2-1-0 to divide；PPARD gene rs2016520, CC-CT-TT genotype is respectively 2-1-0 and divides；MCT1 gene
Rs1049434, TT-AT-AA genotype is respectively 2-1-0 and divides；
5 sites of soft tissue injury risk assessment, its different genotype and being entered as: MMP3 gene rs591058, GG-
AG-AA genotype is respectively 2-1-0 and divides；MMP3 gene rs679620, GG-AG-AA genotype is respectively 2-1-0 and divides；COL5A1
Gene rs12722, TT-CT-CC genotype is respectively 2-1-0 and divides；COL1A1 gene rs1800012, GG-GT-TT genotype is divided
Do not divide for 2-1-0；GDF5 gene rs143383, TT-CT-CC genotype is respectively 2-1-0 and divides；
11 sites of risk of obesity assessment, its different genotype and being entered as: MC4R gene rs17782313, CC-CT-
TT genotype is respectively 2-1-0 and divides；LEPR gene rs8179183, CC-CG-GG genotype is respectively 2-1-0 and divides；FABP2 gene
Rs1799883, AA-AG-GG genotype is respectively 2-1-0 and divides；FTO gene rs1421085, CC-CT-TT genotype is respectively 2-
1-0 divides；PPARG gene rs1801282, GG-CG-CC genotype is respectively 2-1-0 and divides；INSIG2 gene rs7566605, CC-
CG-GG genotype is respectively 2-1-0 and divides；GNB3 gene rs5443, TT-CT-CC genotype is respectively 2-1-0 and divides；ADRB2 gene
Rs1042713, AA-AG-GG genotype is respectively 0-1-2 and divides；ADRB2 gene rs1042714, GG-CG-CC genotype is respectively
2-1-0 divides；ADRB3 gene rs4994, CC-CT-TT genotype is respectively 2-1-0 and divides；UCP1 gene rs1800592, CC-CT-
TT genotype is respectively 2-1-0 and divides；
6 sites of carbohydrate sensitivity assessment, its different genotype and being entered as: TCF7L2 gene
Rs7903146, TT-CT-CC genotype is respectively 2-1-0 and divides；TCF7L2 gene rs12255372, TT-GT-GG genotype is respectively
Divide for 2-1-0；FTO gene rs9939609, AA-AT-TT genotype is respectively 2-1-0 and divides；PPARG gene rs1801282, CC-
CG-GG genotype is respectively 2-1-0 and divides；KCNJ11 gene rs5219, TT-CT-CC genotype is respectively 2-1-0 and divides；IGF2BP2
Gene rs4402960, AA-AC-CC genotype is respectively 2-1-0 and divides；
7 sites of lipid sensitivity assessment, its different genotype and being entered as: APOC1 gene rs4420638, GG-
AG-AA genotype is respectively 2-1-0 and divides；APOB gene rs693, TT-CT-CC genotype is respectively 2-1-0 and divides；LDLR gene
Rs6511720, GG-GT-TT genotype is respectively 2-1-0 and divides；CETP gene rs5882, AA-AG-GG genotype is respectively 2-1-
0 point；CETP gene rs708272, AA-AG-GG genotype is respectively 2-1-0 and divides；Lpl gene rs328, CC-CG-GG genotype
It is respectively 2-1-0 to divide；APOA5 gene rs662799, CC-CT-TT genotype is respectively 2-1-0 and divides.
Preferably, assessment result includes sport potential and deficiency, and wherein sport potential is according to endurance, explosive strength element
Matter is marked.
Another goal of the invention of the present invention is to provide a kind of motion of based on qPCR typing method detecting and evaluating genes system
System, it is characterised in that motion detecting and evaluating genes system includes:
-personal information database, is used for process personal information of collecting and classify；
-motion gene test result database, for detecting the motion gene polymorphism sites of testee, collects detection knot
Really；
-motor capacity or vulnerability database, for gene test result is carried out Contribution Analysis, obtain assessment result；
-motion talent's gene bank, according to the comprehensive remittance of personal information, gene test result, motor capacity or Risk Results
Always, provide corresponding individuation motion or weight management program for person under inspection, and set up motion talent's gene database.
Preferably, gene contribution degree score is normalized, and full marks are 100 points, for a person under inspection, each
The genotype that SNP site obtains according to detection, has a score value, and we define this score value is S, totally 8 projects, Mei Yixiang
We assume that there be n site, then each project must be divided into P:
The score of project is normalized by we according to hundred-mark system, must be divided into P after process100:
P100=(P*100)/2n (2)
It is the most right that person under inspection is carried out classification, ability or risk class in the ability of this project or risk according to score by us
A-B-C-D-E should be identified, represent Gao-slightly higher-normal-the most low-low five grades respectively.
Preferably, motor capacity comprehensive assessment includes that sport potential and motion are less than two aspects.Sport potential is according to endurance
Quality and explosive strength quality are marked, when both be B level and above time, it is believed that this person under inspection's integrated motion ability is high, definition
It it is I rank；When both are C level, it is believed that this person under inspection's integrated motion ability is general, is defined as II rank；When both are D
Level and following time, it is believed that this person under inspection's integrated motion ability is relatively low, is defined as III rank；Sport potential is assessed according to endurance
Marking with explosive strength quality, when endurance is superior to explosive strength rank, this person under inspection is endurance advantage type；Work as explosive force
When amount is superior to endurance rank, this person under inspection is explosive strength advantage type；When explosive strength rank is equal to endurance rank,
This person under inspection has type concurrently for endurance and explosive strength.
Discovery potentiality with the not enough score according to person under inspection in disparity items, draw and are examined compared with crowd's average mark
The ability of person and risk class, ability higher (A level, B level) or risk relatively low (D level, E level) are potentiality, ability relatively low (D level, E
Level) or risk more a height of (A level, B level) deficiency.
According to motor capacity comprehensive assessment, formulate type of sports selection scheme.Endurance and explosive strength have I type concurrently, it is proposed that
Preferably endurance and explosive strength has type project concurrently, secondary selects endurance type project or explosive strength type project；Endurance is held concurrently with explosive strength
Have II type and III type, it is proposed that select endurance and explosive strength to have type project concurrently；Endurance advantage I type, it is proposed that preferably endurance type project,
Secondary endurance and explosive strength is selected to have type project or explosive strength type project concurrently；Endurance advantage II type and III type, preferably endurance type item
Mesh；Explosive strength advantage I type, it is proposed that preferably explosive strength type project, secondary selects endurance and explosive strength to have type project or endurance concurrently
Amount type project；Explosive strength advantage II type and III type, it is proposed that select explosive strength type project.According to potentiality and not enough project, system
Surely elicit latent faculties, improve deficiency, reduce risk training scheme.
Being different from prior art, technique scheme can be carried out all sidedly in motor capacity and relevant risk to person under inspection
Assessment, it is accurate that basic accurate, the assessment efficiency height of assessment and assessment result are stablized.According to gene test result, can be shown that tool
The height of the latent energy value that body person under inspection's genotype is possessed in certain motion.Report in conjunction with gene test, concrete in conjunction with individual
Individual sports are made science, system, are planned targetedly by situation and demand, farthest play the fortune that gene is contained
Dynamic potentiality.During recruiting athlete, existing selection means can be assisted to carry out athlete and to select；During training, can
Athlete to be done individual training guidance and management.The method that instant invention overcomes the single solution for diverse problems of traditional method, athlete
Select and training, and individual body-building effect aspect more advantage.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of the motion detecting and evaluating genes system of the present invention；
Fig. 2 is the content schematic diagram of the motion examining report described in detailed description of the invention.
Detailed description of the invention
By describing the technology contents of technical scheme, structural feature in detail, being realized purpose and effect, below in conjunction with concrete real
Execute example and coordinate accompanying drawing to be explained in detail.
Embodiment 1:
Refer to Fig. 1～2, as the present invention one preferred embodiment, the present embodiment based on qPCR typing skill
The motion detecting and evaluating genes method and system of art include following ingredient:
A. personal information database, personal information database includes individual name, sex, age, height, body weight, BMI
And the personal information such as sample number；
B. motion gene test result database: detect gene based on qPCR typing method, including endurance, explosive force
Amount quality, soft tissue injury risk, sportive recovery from fatigue, motion enthusiasm, risk of obesity, carbohydrate and lipid sensitivity 8
52 pleomorphism sites of 44 related genes in terms of individual quality, obtain the genotype of each person under inspection, and concrete site is such as
Shown in following table:
Endurance: endurance refers to that body keeps the ability of certain strength load or quality of movement within a certain period of time, right
For distance sports events, project that endurance is required particularly important.Outstanding endurance associates with gene pleiomorphism
Research carry out the earliest, and study the most extensive.The gene being associated with outstanding endurance mainly had
Peroxisome proliferator activated receptor gene PPARA and PPARD, adrenergic receptor gene ADRB2, collagen protein coding base
Because of COL5A1, ACE, mtDNA etc..Such as adrenoceptor (Adrenaline receptors, ADR) can with epinephrine and
The g protein coupled receptor that norepinephrine combines.Catecholamine activates adipolytic difference and epinephrine alpha-2 receptor
Affinity is relevant with number.Research finds that marathon runner's utilization rate to fat is just apparently higher than ordinary person and other motions
Member, the significant energy metabolic pathway of fat splitting energy supply advantage endurance exercise.
Explosive strength quality refers to the power making apparatus (or human body itself) move to the most remote distance within the shortest time.
Explosive strength quality is one of two importances of outstanding motor capacity, for short distance speed project, has explosive force and wants
For the project asked particularly important.Explosive strength quality is carried out later with the association study of gene pleiomorphism, has now been found that
ACTN3, ACE, IL6, HIF1A are relevant with explosive force strength characteristics with NOS3 gene etc..Such as ACTN3 gene code α-accessorius
Filamentous actin 3, is only expressed in fast muscle fiber, and fast muscle fiber can produce the contraction required for explosive force.Multiple studies have shown that
R577X polymorphism can affect the myofibrillar distribution proportion of speed.If people's genotype is RR type, the contraction work of fast muscle fiber
Can be normal, explosive strength is strong, also shows as strength building sensitive, so needs prompt explosion power project in dash, weight lifting
In athlete, the ratio of carrying of this normal gene is up to 92%.If genotype is RX type, also have normal inside fast muscle fiber
ACTN3 express, function also will not be impacted.If genotype is XX type, the contraction of fast muscle fiber will be impacted, strength water
Aobvious reduction dawn.
Soft tissue injury, refer to soft tissue or skeletal muscle by direct or indirect violence, or long-term chronic strain cause
A big class wound syndrome.There is microcirculation disturbance, aseptic inflammation after being wound in tissue, and causes local swelling pain.Such as
The MMP3 of MMP3 gene code, the topmost function of this enzyme is exactly degradation of cell epimatrix and basement membrane, its degraded
Substrate include multiple collagen protein (tendon etc. knot hoof tissue), gelatin, Laminin lens, fibronectin, proteoglycan
Deng extracellular matrix components, may participate in damage and the reparation of multiple soft tissue.
Sportive recovery from fatigue: based on energy consuming process during motion, recovery process does not catches up with consumption process, makes ATP store up
Leave fall, muscle glycogen and hepatic glycogen to consume the most in a large number, blood sugar level even can be caused to decline, body central fatigue can be caused,
It is also tired main cause that the accumulation of metabolite is primarily referred to as the increase of lactate level and ammonia content, the speed that lactic acid eliminates
The quantity and the reset mode that produce with it are relevant, and it is the fastest that the lactic acid formed during work eliminates less.Such as adenylic acid takes off
Ammonia enzyme 1 (AMPD1) is all expressed in all of tissue, but main expression in muscular tissue, its effect is catalysis adenosine one
Phosphoric acid (AMP) hydrolytic deaminzation generates inosinic acid (IMP) and ammonia.AMPD1 gene is easier to undergo mutation, the shortage meeting of AMPD1 gene
Cause a kind of metabolic muscle disease, such as the fatigue of exercise induced, cramp, myalgia etc., the blood ammonia caused with purine metabolic disturbance
Level relevant.The activation of AMPD1 and adenosine energy convert can protect body from stress infringement.
Motion enthusiasm: in sports, athletic sports achievement height is relevant with factors, undeniable psychology
Factor has very important impact in training, sports, stand and the many-side such as motivation of pain.Although inherited genetic factors determines the heart
Reason response is also knows about seldom, but has now been found that multiple gene is relevant with athletic motion enthusiasm.Such as BDNF gene, i.e.
Brain Derived Neurotrophic Factor (brain-derived neurotrophic factor, BDNF), it is that one has neural battalion
Support the protein of effect.Brain Derived Neurotrophic Factor and receptor thereof are in nervous system wide expression.It is to hippocampus, ridge in brain
The generation of the neural meaning blood vessel of marrow and skeletal muscle, develop and regenerate and all play an important role.There are some researches show, on BDNF gene
Emotion and the cognition of training that SNP site is different from individuality are relevant.
Risk of obesity: scientific investigations showed that, there is contacting of countless ties with gene in obesity, based on the difference between individuality,
Inherited genetic factors is 20%-70% on fat impact.So, these " ob genes " the most just determine the fat of a human body type
Thin？Answer is clearly negative！One of key factor that gene genetic is simply causeed fat.The appearance of any obesity, be all
Gene and the coefficient result of environment, bad dietary habit (too fast, breakfast skipping, the frequent sumptuous meal etc. eaten), life are practised
The increase of the risk that used (stay up late, do not have enough sleep, tobacco and wine excessively etc.) and lack exercise (sitting is motionless) all can be causeed fat.Example
Such as FTO gene: be referred to as ob gene, it is found in the earliest 2007, is one of gene the strongest with obesity-related.FTO is
C/EBP family transcribes auxiliary activation factor (transcriptional coactivator), can strengthen C/EBP and promoter sequence
The combination of row, plays a role in Adipocyte Differentiation and Fat Accumulation.Research shows that C/EBP family member divides with adipose cell
Changing relevant, C/EBP makes PECTORAL LIMB SKELETON to Adipocyte Differentiation, accelerates Fat Accumulation.
Carbohydrate is the main source of energy needed for the activity of sustaining life.Carbohydrate sensitivity, refers to that people exists
Long-term evolution adapts to the dietary structure of high protein-low-carb, is easy for after taking in substantial amounts of carbohydrate
Forming obesity, this lacks satiety mainly due to lacking appropriate albumen in food, thus such crowd is also easily generated generation
Thank to problem, such as develop into type 2 diabetes mellitus.Such as TCF7L2 gene is transcription factor 7 analog 2, and TCF7L2 is prevalent in
Tissue, including in ripe beta Cell of islet, but seldom expresses in skeletal muscle.TCFTL2 may beta Cell of islet in early days
Atomization plays an important role, affects normal secretions and the blood glucose regulation of insulin.At present, there is many for various places crowd
The SNPs research of TCF7L2 gene all confirms that rs12255372 and rs7903146 is mostly concerned with type 2 diabetes mellitus.
Lipid sensitivity, refers to adapt to Hi CHO-low-fat dietary structure in long-term evolution, works as absorption
Just it is easily formed obesity after substantial amounts of fat, lacks satiety owing to food lacking appropriate carbohydrate.Therefore such
Crowd is also easily generated metabolic problems, such as develops into cardiovascular and cerebrovascular disease.Lipoprotein lipase (lipoprotein lipase
LPL).The main Physiological Function of LPL is rich in three fat in triglyceride (triglyceride, TG) lipoprotein in hydrolysis blood
Acyl glycerol, the glycerol of generation, fatty acid are available for tissue oxidizing and decompose and provide energy.It Chylomicron (chylomicron,
And the catabolism of very low density lipoprotein (VLDL) (very low density lipoprotein, VLDL) has key CM)
Effect.
The concrete gene loci of above-mentioned aspect is:
1) 13 sites of endurance assessment, its different genotype is: ADRB2 gene rs1042713, AQP1 gene
Rs1049305, AMPD1 gene rs17602729, COL5A1 gene rs12722, GABPB1 gene rs12594956, GABPB1 base
Because of rs7181866, HFE gene rs1799945, KCNJ11 gene rs5219, PPARA gene rs4253778, PPARD gene
Rs2016520, UCP3 gene rs1800849, ACE gene rs4646994, PPARGC1A gene rs8192678.
2) 11 sites of explosive strength competency assessment, its different genotype is: AGT gene rs699 site, AMPD1 gene
Rs17602729, CKM gene rs8111989, HIF1A gene rs11549465, IL6 gene rs1800795, NOS3 gene
Rs2070744, PPARA gene rs4253778, PPARG gene rs1801282, ACE gene rs4646994, ACTN3 gene
Rs1815739, mthfr gene rs1801131.
3) 5 sites of soft tissue injury risk assessment, its different genotype is: MMP3 gene rs591058, MMP3 gene
Rs679620, COL5A1 gene rs12722, COL1A1 gene rs1800012, GDF5 gene rs143383.
4) 6 sites of sportive recovery from fatigue assessment, its different genotype is: AMPD1 gene rs17602729, IL6 gene
Rs1800795, HIF1A gene rs11549465, NAT2 gene rs1208, PPARD gene rs2016520, MCT1 gene
rs1049434。
5) 6 sites of motion enthusiasm assessment, its different genotype is: CREB1 gene rs2253206, C18orf2 gene
Rs8097348, AMPD1 gene rs17602729, PATS2L gene rs12612420, PAPSS2 gene rs10887741, BDNF
Gene rs6265.
6) 11 sites of risk of obesity assessment, its different genotype is: MC4R gene rs17782313, LEPR gene
Rs8179183, FABP2 gene rs1799883, FTO gene rs1421085, PPARG gene rs1801282, INSIG2 gene
Rs7566605, GNB3 gene rs5443, ADRB2 gene rs1042713, ADRB2 gene rs1042714, ADRB3 gene
Rs4994, UCP1 gene rs1800592.
7) 6 sites of carbohydrate sensitivity assessment, its different genotype is: TCF7L2 gene rs7903146,
TCF7L2 gene rs12255372, FTO gene rs9939609, PPARG gene rs1801282, KCNJ11 gene rs5219,
IGF2BP2 gene rs4402960.
8) 7 sites of lipid sensitivity assessment, its different genotype is: APOC1 gene rs4420638, APOB gene
Rs693, LDLR gene rs6511720, CETP gene rs5882, CETP gene rs708272, lpl gene rs328, APOA5 base
Because of rs662799.
C. motor capacity or vulnerability database, motor capacity or vulnerability database are for carrying out the gene loci detected
Assessment, the assignment 2 point the most pure and mild according to genotype in each site, favourable heterozygosis assignment 1 point, without affecting assignment 0 point.Different
Genotype and assignment be respectively as follows:
1) 13 sites of endurance assessment: ADRB2 gene rs1042713, AA-AG-GG genotype is respectively 2-1-0
Point；AQP1 gene rs1049305, CC-CG-GG genotype is divided into divides for 2-1-0；AMPD1 gene rs17602729, CC-CT-TT
Genotype is respectively 2-1-0 and divides；COL5A1 gene rs12722, TT-CT-CC genotype is respectively 2-1-0 and divides；GABPB1 gene
Rs12594956, TT-GT-GG genotype is respectively 2-1-0 and divides；GABPB1 gene rs7181866, CC-CT-TT genotype is respectively
Divide for 2-1-0；HFE gene rs1799945, GG-CG-CC genotype is respectively 2-1-0 and divides；KCNJ11 gene rs5219, CC-
CT-TT genotype is respectively 2-1-0 and divides；PPARA gene rs4253778, GG-CG-CC genotype is respectively 2-1-0 and divides；PPARD
Gene rs2016520, CC-CT-TT genotype is respectively 2-1-0 and divides；UCP3 gene rs1800849, TT-CT-CC genotype is divided
Do not divide for 2-1-0；ACE gene rs4646994, II-DI-DD genotype is respectively 2-1-0 and divides；PPARGC1A gene
Rs8192678, GG-AG-AA genotype is respectively 2-1-0 and divides.
2) 11 sites of explosive strength competency assessment: AGT gene rs699 site, CC-CT-TT genotype is respectively 2-1-0
Point；AMPD1 gene rs17602729, CC-CT-TT genotype is respectively 2-1-0 and divides；CKM gene rs8111989, GG-AG-AA
Genotype is respectively 2-1-0 and divides；HIF1A gene rs11549465, TT-CT-CC genotype is respectively 2-1-0 and divides；IL6 gene
Rs1800795, GG-CG-CC genotype is respectively 2-1-0 and divides；NOS3 gene rs2070744, TT-CT-CC genotype is respectively
2-1-0 divides；PPARA gene rs4253778, GG-CG-CC genotype is respectively 0-1-2 and divides；PPARG gene rs1801282, GG-
CG-CC genotype is respectively 2-1-0 and divides；ACE gene rs4646994, II-DI-DD genotype is respectively 0-1-2 and divides；ACTN3 base
Divide because rs1815739, CC-CT-TT genotype is respectively 2-1-0；Mthfr gene rs1801131, CC-AC-AA genotype is respectively
Divide for 2-1-0.
3) 5 sites of soft tissue injury risk assessment: MMP3 gene rs591058, GG-AG-AA genotype is respectively 2-1-
0 point；MMP3 gene rs679620, GG-AG-AA genotype is respectively 2-1-0 and divides；COL5A1 gene rs12722, TT-CT-CC base
Divide because type is respectively 2-1-0；COL1A1 gene rs1800012, GG-GT-TT genotype is respectively 2-1-0 and divides；GDF5 gene
Rs143383, TT-CT-CC genotype is respectively 2-1-0 and divides.
4) 6 sites of sportive recovery from fatigue assessment: AMPD1 gene rs17602729, CC-CT-TT genotype is respectively 2-
1-0 divides；IL6 gene rs1800795, GG-CG-CC genotype is respectively 2-1-0 and divides；HIF1A gene rs11549465, TT-CT-
CC genotype is respectively 2-1-0 and divides；NAT2 gene rs1208, GG-AG-AA genotype is respectively 2-1-0 and divides；PPARD gene
Rs2016520, CC-CT-TT genotype is respectively 2-1-0 and divides；MCT1 gene rs1049434, TT-AT-AA genotype is respectively
2-1-0 divides.
5) 6 sites of motion enthusiasm assessment: CREB1 gene rs2253206, GG-AG-AA genotype is respectively 2-1-0 and divides；
C18orf2 gene rs8097348, GG-AG-AA genotype is respectively 2-1-0 and divides；AMPD1 gene rs17602729, CC-CT-TT
Genotype is respectively 2-1-0 and divides；PATS2L gene rs12612420, AA-AG-GG genotype is respectively 2-1-0 and divides；PAPSS2 base
Divide because rs10887741, TT-CT-CC genotype is respectively 2-1-0；BDNF gene rs6265, AA-AG-GG genotype is respectively
2-1-0 divides.
6) 11 sites of risk of obesity assessment: MC4R gene rs17782313, CC-CT-TT genotype is respectively 2-1-0
Point；LEPR gene rs8179183, CC-CG-GG genotype is respectively 2-1-0 and divides；FABP2 gene rs1799883, AA-AG-GG
Genotype is respectively 2-1-0 and divides；FTO gene rs1421085, CC-CT-TT genotype is respectively 2-1-0 and divides；PPARG gene
Rs1801282, GG-CG-CC genotype is respectively 2-1-0 and divides；INSIG2 gene rs7566605, CC-CG-GG genotype is respectively
Divide for 2-1-0；GNB3 gene rs5443, TT-CT-CC genotype is respectively 2-1-0 and divides；ADRB2 gene rs1042713, AA-
AG-GG genotype is respectively 0-1-2 and divides；ADRB2 gene rs1042714, GG-CG-CC genotype is respectively 2-1-0 and divides；ADRB3
Gene rs4994, CC-CT-TT genotype is respectively 2-1-0 and divides；UCP1 gene rs1800592, CC-CT-TT genotype is respectively
2-1-0 divides.
7) 6 sites of carbohydrate sensitivity assessment: TCF7L2 gene rs7903146, TT-CT-CC genotype is respectively
Divide for 2-1-0；TCF7L2 gene rs12255372, TT-GT-GG genotype is respectively 2-1-0 and divides；FTO gene rs9939609,
AA-AT-TT genotype is respectively 2-1-0 and divides；PPARG gene rs1801282, GG-CG-CC genotype is respectively 0-1-2 and divides；
KCNJ11 gene rs5219, TT-CT-CC genotype is respectively 2-1-0 and divides；IGF2BP2 gene rs4402960, AA-AC-CC base
Divide because type is respectively 2-1-0.
8) 7 sites of lipid sensitivity assessment: APOC1 gene rs4420638, GG-AG-AA genotype is respectively 2-1-0
Point；APOB gene rs693, TT-CT-CC genotype is respectively 2-1-0 and divides；LDLR gene rs6511720, GG-GT-TT genotype
It is respectively 2-1-0 to divide；CETP gene rs5882, AA-AG-GG genotype is respectively 2-1-0 and divides；CETP gene rs708272, AA-
AG-GG genotype is respectively 2-1-0 and divides；Lpl gene rs328, CC-CG-GG genotype is respectively 2-1-0 and divides；APOA5 gene
Rs662799, CC-CT-TT genotype is respectively 2-1-0 and divides.
For a person under inspection, the genotype that each SNP site obtains according to detection, there is a score value, define this
Score value is S.8 detection projects, each hypothesis has n site, then each project must be divided into P:
According to hundred-mark system, the score of project is normalized, after process, P must be divided into100:
P100=(P*100)/2n (2)
As the present embodiment one preferred embodiment, motor capacity or vulnerability database are with thousand human genome East Asia
The genomic data of crowd is foundation, and East Asia crowd includes 5 segmentation subgroups, is Han nationality of BeiJing, China respectively, the southern china Chinese
Race, Xishuangbanna of China the Dai nationality, Tokyo Japanese and Ho Chi Minh City Jing nationality of Vietnam.Calculate the crowd under different characteristic the highest
Score PmaxWith minimum score Pmin(hundred-mark system), carries out 5 deciles by score interval, and its 4 Along ents are respectively P from small to large1、
P2、P3And P4.Person under inspection is carried out classification, ability or risk class correspondence mark respectively in ability or the risk of this project by us
A-B-C-D-E, A rank is P100≥P4；B rank is P3≤P100＜ P4；C rank is P2≤P100＜ P3；D rank is P1≤P100＜
P2；E rank is P100＜ P1；Represent Gao-slightly higher-normal-the most low-low five grades respectively.
This person under inspection ability in disparity items or risk can be obtained according to the ability of single person under inspection or risk score
Rank, and crowd's percentage ratio, the result of comprehensive disparity items can draw the integration capability of person under inspection, predominant direction and self
Not enough.
Motor capacity comprehensive assessment includes sport potential and less than two aspects.Sport potential is according to endurance and explosive force
Amount quality scoring, as long as when two quality has one of which be B level and above time, it is believed that this person under inspection's integrated motion ability
Height, is defined as I rank；When two quality is C level or one of which be C level and time another quality is D, E level, it is believed that should
Person under inspection's integrated motion ability is general, is defined as II rank；When both be D level and following time, it is believed that this person under inspection comprehensively transports
Kinetic force is relatively low, is defined as III rank；Sport potential type evaluation is comprehensively commented with explosive strength quality according to endurance
Point, when endurance is superior to explosive strength quality rank, this person under inspection is endurance advantage type, particularly may be divided into endurance excellent
I grade of gesture type, II grade of endurance advantage type, III grade of endurance advantage type；When explosive strength quality is superior to endurance rank, should
Person under inspection is explosive strength advantage type；When explosive strength quality rank and endurance rank are same one-level, this person under inspection is
Endurance and explosive strength have type concurrently, particularly may be divided into endurance and explosive strength has I grade of type concurrently, endurance has type II concurrently with explosive strength
Level, endurance and explosive strength have III grade of type concurrently.
Find potentiality with not enough according to person under inspection in the score rank of disparity items, ability higher (A level, B level) or risk
Relatively low (D level, E level) is potentiality, and ability relatively low (D level, E level) or risk more a height of (A level, B level) are not enough.
According to motor capacity comprehensive assessment, formulate type of sports selection scheme.Endurance and explosive strength have I type concurrently, it is proposed that
Preferably endurance and explosive strength has type project concurrently, secondary selects endurance type project or explosive strength type project；Endurance is held concurrently with explosive strength
Have II type and III type, it is proposed that select endurance and explosive strength to have type project concurrently；Endurance advantage I type, it is proposed that preferably endurance type project,
Secondary endurance and explosive strength is selected to have type project or explosive strength type project concurrently；Endurance advantage II type and III type, preferably endurance type item
Mesh；Explosive strength advantage I type, it is proposed that preferably explosive strength type project, secondary selects endurance and explosive strength to have type project or endurance concurrently
Amount type project；Explosive strength advantage II type and III type, it is proposed that select explosive strength type project.According to potentiality and not enough project, system
Surely elicit latent faculties, improve deficiency, reduce risk training scheme.
Endurance type project includes swimming, long-distance running, marathon, bicycle, iron-man triathlon, footrace, all-round, cross-country skiing, north
Two, Europe etc.；Explosive strength type project includes dash, high jump, long-jump, javelin, weight lifting, speed skating, short-track speeding skating etc.；Endurance
Have type project concurrently with explosive strength and include the ball game such as football, basketball, vollyball, shuttlecock, tennis, sailing boat, boat, middle length
Distance swimming, weight lifting, wrestling etc..
D. according to personal information, gene test result, motor capacity or the synthesis of Risk Results, provide for person under inspection
Corresponding individuation motion or weight management program, and set up motion talent's gene database.
As the present embodiment another preferred embodiment, or the base of international elite national according to detection
Cause, reversely evaluates advantage and the deficiency of this assessment system, is saying that athletic gene and sports level are stored into motion talent's number
Behind storehouse, assessment models is carried out regular optimization and upgrading.
Embodiment 2:
Lee person under inspection, collecting sample is buccal swab, by the quantitative fluorescent PCR genotype to relating in embodiment 1
Check order.Concrete sequencing result is as follows:
(1) endurance
The endurance testing result of Lee is: 46.2 points (this crowd's average is 45.0), ability grade: C, C
Level crowd accounts for the 33.7% of total crowd.Endurance aspect genotype call results such as following table:
(2) explosive strength quality
The explosive force testing result of Lee is: 63.6 points (this crowd's average is 44.8), ability grade: A, C level
Crowd accounts for the 1.2% of total crowd.Explosive force aspect genotype call results such as following table:
| Gene name | Genotype | Site effect | Genotype describes |
| AGT | CC | ↑↑ | Useful to explosive strength, Angiotensin II activity is the highest, promotes Skeletal Muscle Growth |
| AMPD1 | CC | ↑↑ | Useful to explosive strength, strengthen energy metabolism speed |
| CKM | AA | ↓ | Unhelpful to explosive strength, muscular energy supply is general |
| HIF1A | CT | ↑ | Useful to explosive strength, glycolysis type flesh ratio improves, and glucose absorption ability strengthens |
| IL6 | GG | ↑↑ | Useful to explosive strength, blood plasma il-6 activity is high, promotes muscle cell metabolism |
| NOS3 | TT | ↑↑ | Useful to explosive strength, vascular function strengthens |
| PPARA | CG | ↑ | Unhelpful to explosive strength, fatty acid oxidation energy supply strengthens |
| PPARG | CC | ↓ | Unhelpful to explosive strength, skeletal muscle glucose absorption efficiency and muscle fiber cross-sectional area are general |
| ACE | DD | ↑↑ | Useful to explosive strength, fast muscle fiber ratio improves, and muscle strength and muscle volume increase |
| ACTN3 | CC | ↑↑ | Useful to explosive strength, fast muscle fiber ratio is high, and muscle contraction ability is strong |
| MTHFR | AA | ↓ | Unhelpful to explosive strength, muscle is increased impact little |
(3) soft tissue injury risk
The soft tissue injury risk supervision result of Lee is: 70.0 points (this crowd's average is 65.3), ability level
Other: B, B level crowd accounts for the 43.1% of total crowd.Soft tissue injury risk aspect genotype call results such as following table:
| Gene name | Genotype | Site effect | Genotype describes |
| MMP3<sup>①</sup> | GG | ↑↑ | Soft tissue injury risk is high |
| MMP3<sup>②</sup> | AG | ↑ | Soft tissue injury risk is higher |
| COL5A1 | CC | ↓ | Soft tissue injury risk is relatively low |
| COL1A1 | GG | ↑↑ | Soft tissue injury risk is higher |
| GDF5 | TT | ↑↑ | Soft tissue injury risk is high |
(4) sportive recovery from fatigue
The sportive recovery from fatigue testing result of Lee is: 58.3 points (this crowd's average is 44.7), ability grade:
B, B level crowd accounts for the 11.3% of total crowd.Sportive recovery from fatigue aspect genotype call results such as following table:
| Gene name | Genotype | Point effect | Genotype describes |
| AMPD1 | CC | ↑↑ | Useful to sportive recovery from fatigue, strengthen energy metabolism speed |
| IL6 | GG | ↑↑ | Useful to sportive recovery from fatigue, strengthen muscle injury recovery capability |
| HIF1A | CT | ↑ | Useful to sportive recovery from fatigue, strengthen glucose metabolism ability |
| NAT2 | AG | ↑ | Useful to sportive recovery from fatigue, harmful substance metabolic capacity is stronger |
| PPARD<sup>②</sup> | CT | ↑ | Useful to sportive recovery from fatigue, strengthen skeletal muscle and absorb glucose ability |
| MCT1 | TT | ↓ | Unhelpful to sportive recovery from fatigue, Lactate Transport ability declines |
(5) motion enthusiasm
The motion enthusiasm testing result of Lee is: 66.7 points (this crowd's average is 48.7), ability grade: B, B
Level crowd accounts for the 30.8% of total crowd.Motion enthusiasm aspect genotype call results such as following table:
| Gene name | Genotype | Site effect | Genotype describes |
| CREB1 | AG | ↑ | Useful to motion enthusiasm, motivation in sports is positive, is more happy to take exercise |
| C18orf2 | GG | ↑↑ | Useful to motion enthusiasm, motivation in sports is positive, is more happy to take exercise |
| AMPD1 | CC | ↑↑ | Useful to motion enthusiasm, motivation in sports is positive, is more happy to take exercise |
| PATS2L | GG | ↓ | Unhelpful to motion enthusiasm, motion enthusiasm is general |
| PAPSS2 | TT | ↑↑ | Useful to motion enthusiasm, motivation in sports is positive, is more happy to take exercise |
| BDNF | AG | ↑ | Useful to motion enthusiasm, motivation in sports is positive, is more happy to take exercise |
(6) risk of obesity
The risk of obesity testing result of Lee is: 22.7 points (this crowd's average is 23.0), ability grade: D, D
Level crowd accounts for the 52.4% of total crowd.Motion enthusiasm aspect genotype call results such as following table:
| Gene name | Genotype | Site effect | Genotype describes |
| MC4R | TT | ↓ | Risk of obesity is low |
| LEPR | GG | ↓ | Risk of obesity is low |
| FABP2 | GG | ↓ | Risk of obesity is low |
| FTO | TT | ↓ | Risk of obesity is low |
| PPARG | CC | ↓ | Risk of obesity is low |
| INSIG2 | GG | ↓ | Risk of obesity is low |
| GNB3 | TT | ↑↑ | Risk of obesity is high |
| ADRB2<sup>①</sup> | AG | ↑ | Risk of obesity is medium |
| ADRB2<sup>②</sup> | CC | ↓ | Risk of obesity is low |
| ADRB3 | TT | ↓ | Risk of obesity is low |
| UCP1 | CC | ↑↑ | Risk of obesity is high |
(7) carbohydrate sensitivity
The risk of obesity testing result of Lee is: 25.0 points (this crowd's average is 29.4), ability grade: D, D
Level crowd accounts for the 55.4% of total crowd.Motion enthusiasm aspect genotype call results such as following table:
| Gene name | Genotype | Site effect | Genotype describes |
| TCF7L2<sup>①</sup> | CC | ↓ | Carbohydrate sensitivity is low |
| TCF7L2<sup>②</sup> | GG | ↓ | Carbohydrate sensitivity is low |
| FTO | TT | ↓ | Carbohydrate sensitivity is low |
| PPARG | CC | ↑↑ | Carbohydrate sensitivity is high |
| KCNJ11 | CT | ↑ | Carbohydrate sensitivity is medium |
| IGF2BP2 | CC | ↓ | Carbohydrate sensitivity is low |
(8) lipid sensitivity
The risk of obesity testing result of Lee is: 64.3 points (this crowd's average is 50.0), ability grade: B, B
Level crowd accounts for the 35.9% of total crowd.Motion enthusiasm aspect genotype call results such as following table:
| Gene name | Genotype | Site effect | Genotype describes |
| APOC1 | AA | ↓ | Lipid sensitivity is low |
| APOB | CT | ↑ | Lipid sensitivity is medium |
| LDLR | GG | ↑↑ | Lipid sensitivity is high |
| CETP<sup>①</sup> | AG | ↑ | Lipid sensitivity is medium |
| CETP<sup>②</sup> | AG | ↑ | Lipid sensitivity is medium |
| LPL | CC | ↑↑ | Lipid sensitivity is high |
| APOA5 | CC | ↑↑ | Lipid sensitivity is high |
The gene test result of 8 aspects of summary, the comprehensive assessment result such as following table of Lee:
| Project name | Point value of evaluation | Crowd's accounting | Talent/degree of risk |
| Motion enthusiasm | 66.7 | 8.9% | Is low/and is lower slightly/ is normal/■ is slightly higher/and is high |
| Explosive strength | 63.6 | 1.0% | Is low/and is lower slightly/ is normal/ is slightly higher/and ■ is high |
| Endurance | 46.2 | 19.6% | Is low/and is lower slightly/■ is normal/ is slightly higher/and is high |
| Soft tissue injury | 70.0 | 20.0% | Is low/and is lower slightly/ is normal/■ is slightly higher/and is high |
| Sportive recovery from fatigue | 58.3 | 11.3% | Is low/and is lower slightly/ is normal/■ is slightly higher/and is high |
| Risk of obesity | 22.7 | 21.2% | Is low/and ■ is lower slightly/ is normal/ is slightly higher/and is high |
| Carbohydrate sensitivity | 25.0 | 37.9% | Is low/and ■ is lower slightly/ is normal/ is slightly higher/and is high |
| Lipid sensitivity | 64.3 | 15.3% | Is low/and is lower slightly/ is normal/■ is slightly higher/and is high |
As can be seen from the above table, the endurance score 46.2 of Lee, the percentage ratio in crowd is 19.6%, assessment etc.
Level is C, and higher than crowd's average level 1.2%, your residing endurance C grade percentage ratio in crowd is 33.7%, because of
This your endurance in crowd without advantage.And your explosive strength quality score 63.6, the percentage ratio in crowd is
1.0%, evaluation grade is A, exceeds crowd's average level 18.8%, and your residing explosive strength quality A grade is in crowd
Percentage ratio is only 1.2%, and therefore his explosive strength quality has clear superiority in crowd.To sum up, Lee belongs to explosive force
Amount advantage type I type is individual, selects the sports events needing strong explosive strength to have obvious innate advantage.
The sports events that explosive strength is stronger is the project that the time is short, action is violent, including dash, high jump, long-jump, mark
Rifle, weight lifting, speed skating, short-track speeding skating etc..
The conclusion drawn according to above-mentioned check and evaluation method, includes the Personalized motion training management scheme of Lee's customization
Below some:
1. explosive force is strong, and endurance is general, belongs to explosive strength advantage type.Choose inclined explosive force type of sports, be more easy to play
Motor capacity advantage；
2. motion enthusiasm is higher, needs to continue to keep your motion enthusiasm and participation；
3. sportive recovery from fatigue is relatively strong, can suitably strengthen intensity and the frequency of training；
4. the movement protection of the soft tissues such as soft tissue injury risk is higher, should be noted that knee joint, heel string, evades damage wind
Danger；
5. risk of obesity is low, low to the sensitivity of carbohydrate group food, but the sensitivity to lipid food
Height, therefore takes in the risk rising that excess fat group food can be causeed fat, and needs fat food in strict control meals
Absorption ratio, can suitably increase the absorption of high-quality protein and carbohydrate group food to carry out body weight control.
The conclusion be given in the present embodiment and suggestion are only conclusion and the suggestion that I needs, any according to detecting this reality
Conclusion and the corresponding suggestion that can provide that after executing the genotype in example, statistical separates out all are considered as relating in the present embodiment
Content, should be in this, as the restriction to the application.Any drawn based on the check and evaluation method and system in the application
Conclusion and suggestion all be considered as within the protection domain of the application.
The present invention can be estimated all sidedly in motor capacity and relevant risk to person under inspection, the basis of assessment precisely,
It is accurate that assessment efficiency height and assessment result are stablized.During recruiting athlete, existing selection means can be assisted to carry out athlete
Select；During training, athlete can be done individual training and instruct and management, instant invention overcomes traditional method
The method imposed uniformity without examining individual cases, in selection of sportsmen and training, and individual body-building effect aspect more advantage.
It should be noted that in this article, the relational terms of such as first and second or the like is used merely to a reality
Body or operation separate with another entity or operating space, and deposit between not necessarily requiring or imply these entities or operating
Relation or order in any this reality.And, term " includes ", " comprising " or its any other variant are intended to
Comprising of nonexcludability, so that include that the process of a series of key element, method, article or terminal unit not only include those
Key element, but also include other key elements being not expressly set out, or also include for this process, method, article or end
The key element that end equipment is intrinsic.In the case of there is no more restriction, statement " including ... " or " comprising ... " limit
Key element, it is not excluded that there is also other key element in including the process of described key element, method, article or terminal unit.This
Outward, in this article, " be more than ", " being less than ", " exceeding " etc. are interpreted as not including this number；More than " ", " below ", " within " etc. understand
For including this number.
Although being described the various embodiments described above, but those skilled in the art once know basic wound
The property made concept, then can make other change and amendment to these embodiments, so the foregoing is only embodiments of the invention,
Not thereby the scope of patent protection of the present invention, every equivalent structure utilizing description of the invention and accompanying drawing content to be made are limited
Or equivalence flow process conversion, or directly or indirectly it is used in other relevant technical fields, the most in like manner it is included in the patent of the present invention
Within protection domain.
Claims (10)
1. a motion detecting and evaluating genes method based on qPCR typing method, it is characterised in that: described gene assessment is
The motion gene polymorphism sites to person under inspection of uniting carries out fluorescent PCR detection, and genotype is carried out score assessment；According to gene
Testing result and the statistical analysis of scoring, draw the assessment result of individuation；Assessment result collects foundation motion talent's gene number
According to storehouse.
Motion detecting and evaluating genes method based on qPCR typing method the most according to claim 1, it is characterised in that fortune
Dynamic gene includes following aspect:
Endurance, explosive strength quality, soft tissue injury risk, sportive recovery from fatigue, motion enthusiasm, risk of obesity, carbon water
Compound and lipid sensitivity.
Motion detecting and evaluating genes method based on qPCR typing method the most according to claim 2, it is characterised in that: resistance to
Power quality includes 13 pleomorphism sites, and explosive strength quality includes 11 pleomorphism sites, and soft tissue injury risk includes 5
Pleomorphism site, sportive recovery from fatigue includes 6 pleomorphism sites, and motion enthusiasm includes 6 pleomorphism sites, risk of obesity bag
Including 11 pleomorphism sites, carbohydrate sensitivity includes 6 pleomorphism sites, and lipid sensitivity includes 7 polymorphic positions
Point.
Motion detecting and evaluating genes method based on qPCR typing method the most according to claim 2, it is characterised in that
13 sites of endurance assessment: ADRB2 gene rs1042713, AQP1 gene rs1049305, AMPD1 gene
Rs17602729, COL5A1 gene rs12722, GABPB1 gene rs12594956, GABPB1 gene rs7181866, HFE gene
Rs1799945, KCNJ11 gene rs5219, PPARA gene rs4253778, PPARD gene rs2016520, UCP3 gene
Rs1800849, ACE gene rs4646994, PPARGC1A gene rs8192678；
11 sites of explosive strength competency assessment: AGT gene rs699, AMPD1 gene rs17602729, CKM gene
Rs8111989, HIF1A gene rs11549465, IL6 gene rs1800795, NOS3 gene rs2070744, PPARA gene
Rs4253778, PPARG gene rs1801282, ACE gene rs4646994, ACTN3 gene rs1815739, mthfr gene
rs1801131；
5 sites of soft tissue injury risk assessment: MMP3 gene rs591058, MMP3 gene rs679620, COL5A1 gene
Rs12722, COL1A1 gene rs1800012, GDF5 gene rs143383；
6 sites of sportive recovery from fatigue assessment: AMPD1 gene rs17602729, IL6 gene rs1800795, HIF1A gene
Rs11549465, NAT2 gene rs1208, PPARD gene rs2016520, MCT1 gene rs1049434；
6 sites of motion enthusiasm assessment: CREB1 gene rs2253206, C18orf2 gene rs8097348, AMPD1 gene
Rs17602729, PATS2L gene rs12612420, PAPSS2 gene rs10887741, BDNF gene rs6265；
11 sites of risk of obesity assessment: MC4R gene rs17782313, LEPR gene rs8179183, FABP2 gene
Rs1799883, FTO gene rs1421085, PPARG gene rs1801282, INSIG2 gene rs7566605, GNB3 gene
Rs5443, ADRB2 gene rs1042713, ADRB2 gene rs1042714, ADRB3 gene rs4994, UCP1 gene
rs1800592；
6 sites of carbohydrate sensitivity assessment: TCF7L2 gene rs7903146, TCF7L2 gene rs12255372, FTO
Gene rs9939609, PPARG gene rs1801282, KCNJ11 gene rs5219, IGF2BP2 gene rs4402960；
7 sites of lipid sensitivity assessment: APOC1 gene rs4420638, APOB gene rs693, LDLR gene rs6511720,
CETP gene rs5882, CETP gene rs708272, lpl gene rs328, APOA5 gene rs662799.
Motion detecting and evaluating genes method based on qPCR typing method the most according to claim 1, it is characterised in that every
The contribution margin of individual pleomorphism site is: capability evaluation pleomorphism site is composed according to the favourable pure and mild assignment of genotype 2 points, favourable heterozygosis
It is worth 1 point, without affecting assignment 0 point；Risk assessment pleomorphism site is according to the pure and mild assignment of genotype risk 2 points, risk heterozygosis assignment 1
Point, without affecting assignment 0 point.
Motion detecting and evaluating genes method based on qPCR typing method the most according to claim 1, it is characterised in that: comment
Estimating result and include sport potential and deficiency, wherein sport potential is according to endurance, the scoring of explosive strength quality；Not enough according to being subject to
Inspection person is potentiality in the score rank of disparity items, ability A level～B level or risk D level～E level, ability D level～E level or risk A
～B level is not enough.
Motion detecting and evaluating genes system based on qPCR typing method the most according to claim 1, it is characterised in that fortune
Dynamic detecting and evaluating genes system includes:
-personal information database, is used for process personal information of collecting and classify；
-motion gene test result database, for detecting the motion gene polymorphism sites of testee, collects testing result；
-motor capacity or vulnerability database, for gene test result is carried out Contribution Analysis, obtain assessment result；
-motion talent's gene bank, according to personal information, gene test result, motor capacity or the synthesis of Risk Results, for
Person under inspection provides corresponding individuation motion or weight management program, and sets up motion talent's gene database.
Motion detecting and evaluating genes system based on qPCR typing method the most according to claim 7, it is characterised in that base
Because contribution degree score is normalized, full marks are 100 points.
Motion detecting and evaluating genes system based on qPCR typing method the most according to claim 7, it is characterised in that every
One gene contribution degree score is divided into five grades, ability or risk class correspondence mark A-B-C-D-E respectively, represent Gao-summary respectively
High-normal-the most low-low five grades.
Motion detecting and evaluating genes system based on qPCR typing method the most according to claim 7, it is characterised in that
When endurance is superior to explosive force/strength rank, person under inspection is endurance advantage type；When explosive force/strength is superior to endurance
During rank, person under inspection is explosive strength advantage type；When explosive force/strength rank is equal to endurance rank, person under inspection is endurance
Type is had concurrently with explosive strength.
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| PCT/CN2017/098569 WO2018036495A1 (en) | 2016-08-24 | 2017-08-23 | Qpcr genotyping technique-based athleticism gene detection and evaluation method and system |
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Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101134981A (en) * | 2006-09-01 | 2008-03-05 | 上海体育科学研究所 | Aerobic sport ability related gene polymorphism and detecting chip and reagent case |
| CN101805790A (en) * | 2010-01-26 | 2010-08-18 | 中国人民解放军总医院 | Method for simultaneously detecting polymorphism of 32 SNP loci on 24 sports-related genes |
| WO2010146214A2 (en) * | 2009-06-17 | 2010-12-23 | Progenika Biopharma, S.A. | Method for evaluating the athletic capabilities of a subject |
| CN102864223A (en) * | 2012-08-30 | 2013-01-09 | 山东百福基因科技有限公司 | Detection kit for highly specific multiple genes related to movement functions and detection method thereof |
| CN103160585A (en) * | 2013-04-01 | 2013-06-19 | 哈尔滨体育学院 | Screening method for genes related to physical ability of excellent cross-country skiers |
| CN103201393A (en) * | 2010-11-01 | 2013-07-10 | 霍夫曼-拉罗奇有限公司 | Predicting progression to advanced age-related macular degeneration using a polygenic score |
| CN103525906A (en) * | 2013-08-07 | 2014-01-22 | 广州市体育科学研究所 | Speed or explosive power relative gene-ACTN3 (Alpha-Actinin 3) polymorphism rapid detection kit and detection method |
| CN104109707A (en) * | 2013-04-22 | 2014-10-22 | 泰州医药城博奥邦科生物科技有限公司 | Human physiologic characteristic gene chip |
| WO2015109021A1 (en) * | 2014-01-14 | 2015-07-23 | Omicia, Inc. | Methods and systems for genome analysis |
| CN105603054A (en) * | 2014-11-17 | 2016-05-25 | 武汉白原科技有限公司 | Human explosive power level detection kit and human explosive power level detection method |
| CN105648048A (en) * | 2014-11-17 | 2016-06-08 | 武汉白原科技有限公司 | A human tolerance level detection kit and a detection method |
| CN105671137A (en) * | 2014-11-17 | 2016-06-15 | 武汉白原科技有限公司 | Detection kit and computing method of exercise heart rate response |
| CN105671131A (en) * | 2014-11-17 | 2016-06-15 | 武汉白原科技有限公司 | Detection kit for evaluating human body VO2MAX (Maximum Oxygen Consumption) and method |
| CN105671135A (en) * | 2014-11-17 | 2016-06-15 | 武汉白原科技有限公司 | Detection kit for constitution recovery condition after body building exercises |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103160592B (en) * | 2013-04-03 | 2015-01-14 | 哈尔滨体育学院 | Molecular biology method for predicating endurance potentials of excellent ice-snow sportsmen |
| CN105671136A (en) * | 2014-11-17 | 2016-06-15 | 武汉白原科技有限公司 | Detection kit and computing method for assessing maximal heart rate and target heart rate of human body |
| CN106086222A (en) * | 2016-08-24 | 2016-11-09 | 厦门美因生物科技有限公司 | Motion detecting and evaluating genes method and system based on qPCR typing method |
-
2016
- 2016-08-24 CN CN201610711918.6A patent/CN106086222A/en active Pending
-
2017
- 2017-08-23 WO PCT/CN2017/098569 patent/WO2018036495A1/en active Application Filing
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101134981A (en) * | 2006-09-01 | 2008-03-05 | 上海体育科学研究所 | Aerobic sport ability related gene polymorphism and detecting chip and reagent case |
| WO2010146214A2 (en) * | 2009-06-17 | 2010-12-23 | Progenika Biopharma, S.A. | Method for evaluating the athletic capabilities of a subject |
| CN101805790A (en) * | 2010-01-26 | 2010-08-18 | 中国人民解放军总医院 | Method for simultaneously detecting polymorphism of 32 SNP loci on 24 sports-related genes |
| CN103201393A (en) * | 2010-11-01 | 2013-07-10 | 霍夫曼-拉罗奇有限公司 | Predicting progression to advanced age-related macular degeneration using a polygenic score |
| CN102864223A (en) * | 2012-08-30 | 2013-01-09 | 山东百福基因科技有限公司 | Detection kit for highly specific multiple genes related to movement functions and detection method thereof |
| CN103160585A (en) * | 2013-04-01 | 2013-06-19 | 哈尔滨体育学院 | Screening method for genes related to physical ability of excellent cross-country skiers |
| CN104109707A (en) * | 2013-04-22 | 2014-10-22 | 泰州医药城博奥邦科生物科技有限公司 | Human physiologic characteristic gene chip |
| CN103525906A (en) * | 2013-08-07 | 2014-01-22 | 广州市体育科学研究所 | Speed or explosive power relative gene-ACTN3 (Alpha-Actinin 3) polymorphism rapid detection kit and detection method |
| WO2015109021A1 (en) * | 2014-01-14 | 2015-07-23 | Omicia, Inc. | Methods and systems for genome analysis |
| CN105603054A (en) * | 2014-11-17 | 2016-05-25 | 武汉白原科技有限公司 | Human explosive power level detection kit and human explosive power level detection method |
| CN105648048A (en) * | 2014-11-17 | 2016-06-08 | 武汉白原科技有限公司 | A human tolerance level detection kit and a detection method |
| CN105671137A (en) * | 2014-11-17 | 2016-06-15 | 武汉白原科技有限公司 | Detection kit and computing method of exercise heart rate response |
| CN105671131A (en) * | 2014-11-17 | 2016-06-15 | 武汉白原科技有限公司 | Detection kit for evaluating human body VO2MAX (Maximum Oxygen Consumption) and method |
| CN105671135A (en) * | 2014-11-17 | 2016-06-15 | 武汉白原科技有限公司 | Detection kit for constitution recovery condition after body building exercises |
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|---|---|---|---|---|
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| CN106868126A (en) * | 2017-02-20 | 2017-06-20 | 深圳美因临床检验所有限公司 | Fluorescent quantificationally PCR detecting kit and detection method |
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| CN106868126B (en) * | 2017-02-20 | 2020-06-19 | 深圳美因医学检验实验室 | Fluorescent quantitative PCR detection kit and detection method |
| CN109086563A (en) * | 2017-06-14 | 2018-12-25 | 达易特基因科技股份有限公司 | Using the adaptive physical education analysis method of genetic test |
| CN107391908A (en) * | 2017-06-28 | 2017-11-24 | 天方创新（北京）信息技术有限公司 | Exercise risk appraisal procedure and device |
| CN107893120A (en) * | 2017-11-28 | 2018-04-10 | 保定佑安生物科技有限公司 | The detection method and application of the primer sets of detection motion gene SNP and its application and product and detection motion gene SNP |
| CN107893120B (en) * | 2017-11-28 | 2021-05-04 | 保定佑安生物科技有限公司 | Primer group for detecting motion gene SNP, application and product thereof, and detection method and application for detecting motion gene SNP |
| CN107723370A (en) * | 2017-11-30 | 2018-02-23 | 深圳美因医学检验实验室 | A kind of fluorescence quantitative PCR detection system and its application for nasopharyngeal carcinoma gene screening |
| CN109846040A (en) * | 2018-01-10 | 2019-06-07 | 中创寰宇生物科技有限责任公司 | Use of balsam pear seed oil for preparing anti-body fat forming preparation |
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| CN108893542A (en) * | 2018-06-12 | 2018-11-27 | 广州中安基因科技有限公司 | A kind of movement potential quality and Protecting gene detection kit |
| CN108841966A (en) * | 2018-06-12 | 2018-11-20 | 广州中安基因科技有限公司 | A kind of weight reducing gene detecting kit |
| CN108676850A (en) * | 2018-06-12 | 2018-10-19 | 广州中安基因科技有限公司 | A kind of exercise metabolism gene detecting kit |
| CN108998542A (en) * | 2018-08-30 | 2018-12-14 | 上海佰臻生物科技有限公司 | A kind of appetite ability gene appraisal procedure |
| CN109468388A (en) * | 2018-12-25 | 2019-03-15 | 北京北基医学检验实验室有限公司 | A kind of primer sets and detection kit of amplification and locomitivity related gene |
| CN110358844A (en) * | 2019-08-13 | 2019-10-22 | 沈阳哥瑞科技有限公司 | Movement genetic marker site detection method and products thereof based on nucleic acid mass spectrum typing method |
| CN112941191A (en) * | 2019-12-11 | 2021-06-11 | 宁波海尔施基因科技有限公司 | Multiple gene kit for detecting movement pattern and use method thereof |
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