CN108670497A - A kind of closed type rat breathing mask - Google Patents

A kind of closed type rat breathing mask Download PDF

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Publication number
CN108670497A
CN108670497A CN201810566564.XA CN201810566564A CN108670497A CN 108670497 A CN108670497 A CN 108670497A CN 201810566564 A CN201810566564 A CN 201810566564A CN 108670497 A CN108670497 A CN 108670497A
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China
Prior art keywords
rat
ratch
shaped
mask
special
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CN201810566564.XA
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Chinese (zh)
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CN108670497B (en
Inventor
赵渊
蒋华北
葛绍理
赵芮
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University of Electronic Science and Technology of China
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University of Electronic Science and Technology of China
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D7/00Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
    • A61D7/04Devices for anaesthetising animals by gases or vapours; Inhaling devices

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Anesthesiology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Respiratory Apparatuses And Protective Means (AREA)

Abstract

The invention discloses a kind of closed type rat breathing masks, are related to a kind of animal experiment device, more particularly to a kind of rat breathing mask.The problem of the invention solves effective fixed rat head and preventing mask internal gas from leaking.The present invention is by special-shaped ar bowl-shaped enclosure, special-shaped silica gel sealing ring, ratch, silica gel ring and packing ring composition.Special-shaped ar bowl-shaped enclosure binding silica gel sealing ring enables mask to be bonded rat face, and ratch connects rat and, also as the outlet passageway of mask, packing ring completes the sealing to gas outlet while can effectively fixing ratch while breathing mask.The good leakproofness of the present invention can not only effectively prevent experimenter to be exposed in hazardous gas, it avoids ambient atmos (liquid) from entering mask, and optoacoustic, thermal acoustic imaging can be met etc. experiment also ensures its experiment demand freely breathed after rat pulls the plug.

Description

A kind of closed type rat breathing mask
Technical field
The present invention relates to a kind of animal experiment device, more particularly to a kind of rat breathing mask.
Background technology
In medicine and life science, breathing mask is being inhaled as a kind of important device in rat experiment Enter and is widely used in the experiments such as formula anesthesia, inhalation-type drug administration, the exposure of mouth and nose smog.Existing rat breathing mask is mainly brain The tooth of rat, is usually placed on ratch by stereotaxic instrument, the equipment developments such as Magnetic resonance imaging, by cone (or circle Cylindricality) sucting mask moves back up to cling to rat nose or move down by fixed block and pushes down rat nose Realize that rat head is fixed.However, the head of rat is not completely conical, the face of animal cannot complete binding face The dangerous leakage of anesthetic gases occurs in order to prevent for cover, and one layer of recycling mask can be also designed generally outside circular cone sucting mask, Cooperation gas recovery device siphons away the anesthetic gases of spilling.However this method needs the flow for increasing anesthetic gases to ensure rat It breathes, while needing expensive recover to generate negative pressure around sucting mask to prevent gas overflowing, wastes part fiber crops Medicine increases the equipment cost mating with rat breathing mask.Further, since mask seal is bad, cause depth of anesthesia, The problems such as gas and drug concentration control and difficult monitoring.Therefore closed type rat mask can reduce anaesthetic waste, save back The cost of device is received, while ambient atmos being avoided to be mixed into mask, gas concentration is allowed more to stablize, gas componant monitoring is simpler.
Other than above application has the demand for preventing mask gas overflowing, in imaging fields such as photoacoustic imaging, thermal acoustic imagings In, penetrate into the demand of breathing mask there is also extraneous gas and liquid is prevented.For example, when carrying out optoacoustic, thermal acoustic imaging, rat Scanned position, which needs to be completely immersed in water, to be detected by ultrasonic probe, when needing to scan the brain of rat, in order to ensure Rat can breathe, and can only be positioned over rat outside water, and signal detection is completed by film and ultrasonic coupling agent, and film Laser, microwave and ultrasound can be reflected, scattered and absorbed with ultrasonic coupling agent, makes the optoacoustic of acquisition, the matter of thermoacoustic image Quantitative change is poor.Wearing closed type rat mask can prevent couplant (such as deionized water) from entering inside mask, make rat can Directly to put it into water optoacoustic, the thermal acoustic imaging experiment for carrying out the position in addition to mouth and nose in the case where freely breathing.Greatly Mouse skin can be contacted directly with couplant, be interfered caused by avoiding film and ultrasonic coupling agent.It therefore can by the present invention To realize a kind of new live body rat imaging method based on optoacoustic and thermo-acoustic technology.
Invention content
The object of the present invention is to provide a kind of closed type rat breathing masks, can be the position hand in addition to rat mouth and nose Art, treatment and imaging etc. need the research of suction-type anesthesia (administration) to provide an effectively fixed rat head and sealing performance Reliable rat breathing mask.The harm of gas overflowing is prevented, or ambient atmos (liquid) is avoided to enter mask.Meet optoacoustic, The experiment such as thermal acoustic imaging is fully submerged the experiment demand in water in the case where ensureing rat eupnea.
As depicted in figs. 1 and 2, technical solution of the present invention is a kind of closed type rat breathing mask, which includes:Abnormity Ar bowl-shaped enclosure, is set to special-shaped ar bowl-shaped enclosure bottom at the special-shaped silica gel sealing ring being tightly connected with special-shaped ar bowl-shaped enclosure peristoma Air inlet pipe and an air outlet pipe, the hollow ratch being set in escape pipe, the ratch clamp device being set to outside escape pipe;The tooth Bar one end is located in shell, and one end is located at outside shell, and front tooth mounting hole is offered on one end ratch in shell, for solid Determine the front tooth of rat;Mask is worn when on rat head, the air inlet pipe position corresponds to rat nose, and escape pipe position corresponds to Rat face.The abnormity ar bowl-shaped enclosure and special-shaped silica gel sealing ring are consistent with rat facial dissection profile.
Further, the ratch clamp device is:Packing ring with arc surface, is arranged in escape pipe elastic fastening circle The external screw thread of outside, the internal thread for being set to one end inside packing ring;The other end is gradually reduced for radius inside the packing ring Arc surface structure, the centrally disposed trepanning of arc surface;The external screw thread outside internal thread and escape pipe inside the packing ring is nibbled Close, on packing ring the trepanning at arc surface end allow ratch to pass freely through, elastic fastening circle is set in the arc surface of packing ring.Institute Stating packing ring, there are two effects by the generation deformation of screw thread cap extruding silica gel sealing ring:(a) compressing is mounted on ratch Silicone tube achievees the purpose that fixed ratch, (b) seals gas outlet peripheral gap.
Further, the ratch is divided into two sections:One section is cylinder, and another section is the quadrangular for being provided with wedge step Shape, front tooth mounting hole are set to the end of four prism type, and the escape pipe inner passage ratch quadrangular coordinates, inside escape pipe It is provided with and is used to stop that ratch all to slide into inside mask with the wedge step that wedge-shaped platform matches on ratch, the wedge step.
Further, the air inlet pipe outer wall is provided with circumferential barb slot, and the hose for preventing from connecting with air inlet pipe is de- It falls.
Further, the escape pipe lower part of the shell is provided with circular locating slot, the angle for adjusting rat head.
Further, the elastic fastening circle is silica gel ring.
Further, the rat breathing mask includes following three kinds of sizes:
(1) it is suitable for 100g-200g rats:A height of 25mm × the 16.4mm of length and width × 21.6mm of special-shaped ar bowl-shaped enclosure, into Gas port is internal diameter 3mm, outer diameter 5mm, and the cylinder of 5.5mm is stretched out from mask surface;Gas outlet outer diameter 10mm, extend out to and air inlet Mouth is in conplane cylinder;It is the prismatic trepanning of 5mm × 5mm inside gas outlet, outside is M10 screw threads;Under gas outlet There is the circular locating slot of diameter 4mm depths 3mm in portion;Packing ring outer diameter 13mm, inside are the screw thread of M10, and packing ring opening diameter is 6mm, inside are the fillet that radius is 2.5mm;It is the through-hole of a diameter of 3mm inside ratch, a diameter of 5mm of cylindrical section is long 16m, tetragonous shell of column bottom surface are 5mm × 5mm, long 13mm, and there are one the rat front tooth location holes of 3mm × 5mm thereon;
(2) it is suitable for 200-300g rats:No except special-shaped ar bowl-shaped enclosure length, width and height 31.5mm × 20.5mm × 27mm and (1) Together, remaining structure size is identical as (1);
(3) it is suitable for 300g-500g rats:Except special-shaped ar bowl-shaped enclosure length, width and height 35mm × 23mm × 30mm is different from (1), Remaining structure size is identical as (1).
The present invention is compared with existing scheme, in addition to breathing mask shape is different, also adds special-shaped silica gel sealing ring and tooth Bar fastening seal structure part, wherein ratch fastening seal structure part include the externally threaded gas outlet of rectangular through-hole in mask, urgent Ring and elastic fastening coil structures.In addition, the ratch of prior art is solid, an individually designed gas outlet is needed, this Innovation gas outlet is integrated into inside ratch, ratch, which is designed as through-hole structure, eliminates a gas outlet structural member, So that breathing mask structure is compacter while realizing mask seal.The shape of the present invention meets rat facial dissection wheel Exterior feature reduces respiration dead space.The ratch fastening seal structure of innovation designs, and keeps face mask structure compact, small and exquisite.Face is needed with existing The method that rat head could be fixed with ear bar by covering is compared, and the present invention is easy to use, and rat head can be fixed using only mask Portion.Increased abnormal shape silica gel sealing ring structure design enhances compatibility and sealed reliable of the mask for different building shape rat Property.With it is existing needs by entire rat head it is fully wrapped around could realize the method for leakproofness compared with, the present invention has only been blocked greatly Mouse mouth and nose, applicability is wider, is suitable for the experimental study at the position such as rat eye, brain, ear.
Description of the drawings
Fig. 1 closed type rat breathing mask stereoscopic schematic diagrams;
Fig. 2 closed type rat breathing mask upward views;
Fig. 3 closed type rat breathing mask diagrammatic cross-sections;
Fig. 4 closed type rat breathing mask decomposition diagrams;
Fig. 5 closed type rat breathing mask scheme of installation.
In figure:1. special-shaped ar bowl-shaped enclosure, 2. special-shaped silica gel sealing rings, 3. ratch, 4. elastic fastening circles, 5. packing rings, 6. The circumferential barb slot of air inlet pipe, 7. circular locating slots, the arc surface inside 8. packing rings, the internal thread of 9. packing rings, 10. outlets External screw thread outside pipe, 11. rats face, the cylindrical segment of 12. ratch, prismatic section of 13. ratch, 14. front tooth mounting holes, 15. silicone tube.
Specific implementation mode
The present invention devises ratch (3), has not only been used to connect rat and mask, but also be used as outlet passageway.Such as Fig. 3, Fig. 4 Shown in Fig. 5, silica gel tracheae (15) is sleeved in the cylindrical structure (12) of ratch (3), and ratch (3) passes through special-shaped ar bowl-shaped enclosure (1) rectangular opening is inserted into mask, and the position of the front tooth mounting hole (14) on ratch (3) is located at rat front tooth institute in the plane, First rat front tooth is fixed in the mounting hole (14) of ratch (3), then ratch is back pulled to rat face and special-shaped silica gel Sealing ring (2) is bonded completely, achievees the effect that sealing.Packing ring (5) is screwed at this time, in screwing operations, inside packing ring Arc surface (8) can squeeze silica gel ring (4), realize the internal thread of the external screw thread (10) and packing ring (5) of special-shaped ar bowl-shaped enclosure (1) With the sealing effect of the hose (15) and the contact surface of packing ring (5) that are sleeved on ratch (3).Rectangular configuration is provided on ratch (13) rectangular outlet with special-shaped ar bowl-shaped enclosure (1) matches, and the ratch rotation inside being driven when rotation packing ring (5) is avoided to make Deviate from from ratch mounting hole at rat front tooth.
Rat breathing mask of the present invention includes following three kinds of sizes:
(1) it is suitable for 100g-200g rats:A height of 25mm × the 16.4mm of length and width × 21.6mm of special-shaped ar bowl-shaped enclosure, into Gas port is internal diameter 3mm, outer diameter 5mm, and the cylinder of 5.5mm is stretched out from mask surface;Gas outlet outer diameter 10mm, extend out to and air inlet Mouth is in conplane cylinder;It is the prismatic trepanning of 5mm × 5mm inside gas outlet, outside is M10 screw threads;Under gas outlet There is the circular locating slot of diameter 4mm depths 3mm in portion;Packing ring outer diameter 13mm, inside are the screw thread of M10, and packing ring opening diameter is 6mm, inside are the fillet that radius is 2.5mm;It is the through-hole of a diameter of 3mm inside ratch, a diameter of 5mm of cylindrical section is long 16m, tetragonous shell of column bottom surface are 5mm × 5mm, long 13mm, and there are one the rat front tooth location holes of 3mm × 5mm thereon;
(2) it is suitable for 200-300g rats:No except special-shaped ar bowl-shaped enclosure length, width and height 31.5mm × 20.5mm × 27mm and (1) Together, remaining structure size is identical as (1);
(3) it is suitable for 300g-500g rats:Except special-shaped ar bowl-shaped enclosure length, width and height 35mm × 23mm × 30mm is different from (1), Remaining structure size is identical as (1).
(1), specific implementation mode one
1, it anaesthetizes:It is injected intraperitoneally using 3% yellow Jackets (40-50mg/kg) or rat is put into the different of 4% concentration Fluothane induces box to carry out gas induced anesthesia.
2, unhairing:According to imaging technique need to reject a part of hair (such as:Optoacoustic, thermoacoustic and ultrasonic imaging), then it needs It will first be become mildewed rejectings with the special shaver of toy to specified region, and reuse depilatory cream and remove undercoat and villus.(if desired The air-tightness for ensureing 100%, the hair in region of needing rat face being in contact with abnormal shape silica gel sealing ring are rejected with shaver, with Ensure that special-shaped silica gel sealing ring 2 is contacted with rat facial skin).
2, ratch 3 and waste gas recovery tank (such as activated carbon recycling can) are connected with silicone tube, ensures that experimenter will not connect Contact exhaust gas.Mask air inlet is connected with silicone tube and with the air pump of flowmeter.If desired suction-type anesthesia is carried out, silicon can be used Sebific duct connects mask air inlet 6 and Anesthesia machine.
3, rat head and mask connect:As shown in figure 5, rat front tooth is put into the mounting hole 14 of ratch 3, pull back Mask and rat head after screwing packing ring, can be connected as one by moving teeth bar 3 up to experiencing light resistance.
4, the circular locating slot 7 of mask is connected with mask fixator:Regulate the posture of rat.
5, the researchs such as position operation, treatment and imaging in addition to rat mouth and nose are carried out.
6, mask is removed:Packing ring 5 is unscrewed, carefully 14 takes out rat front tooth from 3 mounting hole of ratch;With rat Couplant dry, be put into the mouse incubator sterilized.After waiting for anesthesia duration, rat is awake, can freely activity.
(2), specific implementation mode two
A kind of new live body rat imaging method based on optoacoustic and thermo-acoustic technology may be implemented by the present invention.With it is specific Embodiment one is compared, and specific implementation mode two has difference, specific implementation mode in the 5th point of method studied For:
1, the rat for wearing the present invention can be mounted on corresponding rat fixator by circular locating slot 7 through the invention On.
2, after adjusting rat posture by rat fixator, directly entire fixator is put into couplant and carries out light Sound, thermal acoustic imaging scanning meet the experiment such as optoacoustic, thermal acoustic imaging and also ensure its reality freely breathed after rat pulls the plug Test demand.
Compared with existing optoacoustic, thermoacoustic just have film and ultrasonic coupling agent carries out live body rat experimental method, the present invention is real Show rat skin to be directly in contact with couplant, avoids believing laser, microwave and ultrasound for film and ultrasonic coupling agent Number interference, improve the signal-to-noise ratio of image.In addition to this, this method avoid existing methods is positioned over nothing under film by rat The problem of rat posture is adjusted flexibly in method, while avoiding rats breathing difficulty caused by rat is oppressed due to hydraulic pressure, improves Rat imaging experiment survival rate.

Claims (7)

1. a kind of closed type rat breathing mask, the mask include:Special-shaped ar bowl-shaped enclosure connects with special-shaped ar bowl-shaped enclosure peristoma sealing The special-shaped silica gel sealing ring that connects, the air inlet pipe and an air outlet pipe for being set to special-shaped ar bowl-shaped enclosure bottom, in being set in escape pipe Empty ratch, the ratch clamp device being set to outside escape pipe;Described ratch one end is located in shell, and one end is located at outside shell, Front tooth mounting hole is offered on one end ratch in shell, the front tooth for fixing rat;Mask is worn on rat head When, the air inlet pipe position corresponds to rat nose, and escape pipe position corresponds to rat face.
2. a kind of closed type rat breathing mask as described in claim 1, it is characterised in that the ratch clamp device is:Band The packing ring of arc surface, elastic fastening circle, the external screw thread being arranged outside escape pipe, the interior spiral shell for being set to one end inside packing ring Line;The other end is the diminishing arc surface structure of radius, the centrally disposed trepanning of arc surface inside the packing ring;The packing Internal thread inside ring is engaged with the external screw thread outside escape pipe, on packing ring the trepanning at arc surface end allow ratch freely to wear It crosses, elastic fastening circle is set in the arc surface of packing ring.
3. a kind of closed type rat breathing mask as described in claim 1, it is characterised in that the ratch is divided into two sections:One section For cylinder, another section is the four prism type for being provided with wedge step, and front tooth mounting hole is set to the end of four prism type, described The ratch quadrangular cooperation of escape pipe inner passage, the wedge step that escape pipe is internally provided with wedge-shaped platform matches on ratch, The wedge step is for stopping that ratch all slides into inside mask.
4. a kind of closed type rat breathing mask as described in claim 1, it is characterised in that the air inlet pipe outer wall is provided with Circumferential barb slot, for preventing the hose being connect with air inlet pipe from falling off.
5. a kind of closed type rat breathing mask as described in claim 1, it is characterised in that the escape pipe lower part of the shell It is provided with circular locating slot, the angle for adjusting rat head.
6. a kind of closed type rat breathing mask as claimed in claim 2, it is characterised in that the elastic fastening circle is silica gel Circle.
7. a kind of closed type rat breathing mask as claimed in claim 2, it is characterised in that the rat breathing mask includes Three kinds of sizes below:
(1) it is suitable for 100g-200g rats:A height of 25mm × the 16.4mm of length and width × 21.6mm of special-shaped ar bowl-shaped enclosure, air inlet For internal diameter 3mm, outer diameter 5mm, the cylinder of 5.5mm is stretched out from mask surface;Gas outlet outer diameter 10mm, extend out to and exists with air inlet Conplane cylinder;It is the prismatic trepanning of 5mm × 5mm inside gas outlet, outside is M10 screw threads;Gas outlet lower part has The circular locating slot of diameter 4mm depths 3mm;Packing ring outer diameter 13mm, inside are the screw thread of M10, and packing ring opening diameter is 6mm, Inside is the fillet that radius is 2.5mm;It is the through-hole of a diameter of 3mm inside ratch, cylindrical section a diameter of 5mm, long 16m, Tetragonous shell of column bottom surface is 5mm × 5mm, long 13mm, and there are one the rat front tooth location holes of 3mm × 5mm thereon;
(2) it is suitable for 200-300g rats:Except special-shaped ar bowl-shaped enclosure length, width and height 31.5mm × 20.5mm × 27mm is different from (1), Remaining structure size is identical as (1);
(3) it is suitable for 300g-500g rats:Except special-shaped ar bowl-shaped enclosure length, width and height 35mm × 23mm × 30mm is different from (1), remaining Structure size is identical as (1).
CN201810566564.XA 2018-06-05 2018-06-05 Canned type rat respirator Expired - Fee Related CN108670497B (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109745143A (en) * 2019-03-22 2019-05-14 中国科学院深圳先进技术研究院 A kind of anaesthetic mask for animal component
CN110200724A (en) * 2019-07-03 2019-09-06 武汉瑞福宁科技有限公司 A kind of rats and mice gas anesthesia mask
CN111281595A (en) * 2020-03-06 2020-06-16 武汉联影生命科学仪器有限公司 Animal fixing bin and imaging scanning system
RU202204U1 (en) * 2020-07-10 2021-02-05 Федеральное государственное бюджетное образовательное учреждение высшего образования Санкт-Петербургский государственный университет ветеринарной медицины ФГБОУ ВО СПбГУВМ Breathing mask for horses

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CN203662940U (en) * 2013-12-25 2014-06-25 中南大学湘雅医院 Sealed cover for continuous inhalation anesthesia of rat
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CN204379480U (en) * 2015-01-07 2015-06-10 邱德来 The three-dimensional fixture of the laboratory animal brain used under a kind of microscope
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109745143A (en) * 2019-03-22 2019-05-14 中国科学院深圳先进技术研究院 A kind of anaesthetic mask for animal component
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CN110200724A (en) * 2019-07-03 2019-09-06 武汉瑞福宁科技有限公司 A kind of rats and mice gas anesthesia mask
CN111281595A (en) * 2020-03-06 2020-06-16 武汉联影生命科学仪器有限公司 Animal fixing bin and imaging scanning system
RU202204U1 (en) * 2020-07-10 2021-02-05 Федеральное государственное бюджетное образовательное учреждение высшего образования Санкт-Петербургский государственный университет ветеринарной медицины ФГБОУ ВО СПбГУВМ Breathing mask for horses

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