CN108668900A - A kind of tissue cultivation rapid breeding method of wrinkle leaf Alpinia japonica - Google Patents
A kind of tissue cultivation rapid breeding method of wrinkle leaf Alpinia japonica Download PDFInfo
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- CN108668900A CN108668900A CN201810690810.2A CN201810690810A CN108668900A CN 108668900 A CN108668900 A CN 108668900A CN 201810690810 A CN201810690810 A CN 201810690810A CN 108668900 A CN108668900 A CN 108668900A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention discloses a kind of tissue cultivation rapid breeding methods of wrinkle leaf Alpinia japonica.This method includes explant disinfection, adventitious bud inducing, adventitious bud proliferation, strong plantlets and rootage and acclimatization and transplants step.The present invention only needs simple Plant Tissue Breeding equipment that can carry out, and a cycle from the bud point on explant stem tuber to intact plant is completed in 80~120d, greatly accelerates the reproduction speed of wrinkle leaf Alpinia japonica.This method culture is wrinkled, and leaf Alpinia japonica is with short production cycle, proliferation times are high, survival rate is high, can obtain enough test tube plantlets, is conducive to mass market and promotes.
Description
Technical field
The invention belongs to field of plant tissue culture technique, and in particular to a kind of tissue culture quick breeding side of wrinkle leaf Alpinia japonica
Method.
Background technology
The leaf Alpinia japonica (Alpinia rugosa S.J.Chen＆Z.Y.Chen) that wrinkles is Zingiber Alpinia novel species, only produces Hainan and protects
Pavilion hangs Luoshan.It is introduced a fine variety from nineteen ninety to after South China Botanical Garden, domestic Xishuangbanna botanical garden and external Hawaii are planted from south China
It introduces a fine variety in object garden.Hawaii will wrinkle leaf Alpinia japonica breeding application at present.The plant has high gardening ornamental with its leaf shrinkage
Property, it is the novel gardening ornamental plant of Zingiber.Blade is oval, and blade face is hairless, and the back side is close by pubescence, great wrinkle, base portion innermost being
Shape is overlapped, slightly deflection.Raceme is upright, close flower, has 9-20 flower, entire inflorescence is slightly long in fruiting period, carries on the back close yellow pubescence；
Bract brown, calyx pink, tubulose；Because its plant type is neat, blade is peculiar and is easy to cultivation management, can be applied to all kinds of public
Greenery patches, garden and road both sides greenery patches, also can be used as indoor sight leaf and hayashishita ground cover plant.
Leaf Alpinia japonica breeding of wrinkling mainly is bred using cutting root-like stock, and because of the scarcity of species, not excessive plant is for cutting
Divide breeding.And cutting breeding proliferation rate is relatively low, long-term division propagation is easy virus infection, is unfavorable for the excellent hereditary capacity of kind
Stability.Tissue culture propagating survival rate is high, breeding growth rate is high, growth cycle is short, can carry out large-scale breeding in a short time；
However, there has been no the correlation techniques of suitable wrinkle leaf Alpinia japonica tissue culture quick breeding.
Invention content
The purpose of the present invention is to provide a kind of tissue cultivation rapid breeding methods of wrinkle leaf Alpinia japonica, are deposited in the prior art with overcoming
Deficiency, this method culture wrinkle leaf Alpinia japonica it is with short production cycle, proliferation times are high, survival rate is high, enough test tube plantlets can be obtained；
And wrinkle leaf Alpinia japonica seedling stability is improved, the good characteristic of maternal plant is kept.
The tissue cultivation rapid breeding method of the wrinkle leaf Alpinia japonica of the present invention, includes the following steps：
A. explant sterilizes：The wrinkle leaf Alpinia japonica subterranean stem of sprouting is selected, the blade of fibrous root, stem apex, outer layer covers is removed,
Cleaning disinfection, cutting contain the stem tuber of single bud point as explant, carry out disinfection to explant；
B. adventitious bud inducing：Explant is inoculated into adventitious bud induction culture base, Fiber differentiation adventitious bud；It is described not
Normal bud inducing culture：Every liter containing 2.0~3.5mg of 6- benzyl aminoadenines, 0.2~0.3mg of niacin, 20~30g of sucrose,
5.0~6.0g of agar, surplus are MS culture mediums, and pH value is 5.6~5.8；
C. adventitious bud proliferation：Adventitious bud is cut, blade and stem is removed, stays bastem portion, is inoculated into adventitious bud proliferation training
It supports in base, culture forms Multiple Buds；The adventitious bud proliferation culture medium：Every liter containing 6- benzyls aminoadenine 1.5~
3.0mg, 0.1~0.3mg of niacin, 20~30g of sucrose, 5~6g of agar, surplus are MS culture mediums, and pH value is 5.6~5.8；
D. strong plantlets and rootage：Single Multiple Buds are cut, are inoculated into Rooting and hardening-off culture base, culture forms rooted seedling；
The Rooting and hardening-off culture base：Every liter contains 0.3~0.8mg of niacin, 20~30g of sucrose, 5~6g of agar, surplus MS
Culture medium, pH value are 5.6~5.8；
E. acclimatization and transplants：Rooted seedling is taken out, root culture medium is cleaned, the transplantation of seedlings that will take root after sterilized is to cultivation matrix
In, training orientation is carried out, wrinkle leaf Alpinia japonica seedling is obtained.
It is preferred that the cleaning disinfection of the step a is rinsed well with 75% ethanol water of volume fraction；The step
Carry out disinfection to explant in rapid a is that 75% ethanol water of explant volume fraction is impregnated 20~30s, sterile water wash
1 time；0.08%~0.12% mercuric chloride of mass fraction is used to impregnate 8~10min, aseptic water washing 4~6 times again.
It is preferred that the condition of culture in described step b, c, d is：27 ± 2 DEG C, 1800~2300lx of intensity of illumination of temperature,
10~14h/d of light application time.
It is furthermore preferred that the condition of culture in described step b, c, d is：27 ± 2 DEG C, intensity of illumination 2000lx of temperature, light
According to time 12h/d.
It is preferred that the disinfection of the step e is to soak 0.1%~0.3% Bravo solution of rooted seedling use quality score
Steep 30~40min.
It is preferred that the cultivation matrix of the step e be fine sand, perlite and peat soil with weight ratio 1~2：1~2：3~4
The mixture being uniformly mixed so as to obtain.
It is preferred that the condition of culture of the step e is：23~27 DEG C of temperature, relative air humidity 70%~80%.
It is preferred that the adventitious bud induction culture base：Every liter containing 6- benzyl aminoadenines 3.0mg, niacin 0.3mg,
Sucrose 30g, agar 5.5g, surplus are MS culture mediums, pH value 5.8.
It is preferred that the adventitious bud proliferation culture medium：Every liter containing 6- benzyl aminoadenines 2.0mg, niacin 0.15mg,
Sucrose 25g, agar 5.5g, surplus are MS culture mediums, pH value 5.8.
It is preferred that the Rooting and hardening-off culture base：Every liter is containing niacin 0.5mg, sucrose 25g, agar 5.5g, surplus
MS culture mediums, pH value 5.8.
For the present invention using the sprouting to wrinkle on the stem tuber of leaf Alpinia japonica underground as material, subterranean stem is infected with multiple-microorganism；If directly using
Water rinses underground stem tuber, and since stem tuber may be damaged during excavation, tap water flushing is likely to cause wound microbiological contamination
Make explant body pollution, this will be influenced, and follow-up wrinkle leaf Alpinia japonica expansion is numerous, and breeding is slow, survival rate is low.The present invention first to explant stem tuber into
Row cleaning sterilizes, then stem tuber of the cutting with bud point；Plant avoids contact with other substances from after excavating or is infected, through excessive
Secondary to disinfect, explant pollution rate is low, and Disinfection Effect is apparent, this is beneficial to subsequent tissue culture propagating：Greatly shorten
Breeding cycle and the survival rate for improving plant.
The beneficial effects of the present invention are：
(1) present invention is using the sprouting to wrinkle on leaf Alpinia japonica subterranean stem as material, by repeatedly disinfecting, you can enters induction
Phase, the explant pollution rate after disinfecting is low, and the present invention only needs simple Plant Tissue Breeding equipment that can carry out, and utilizes plant
The biotechnologys such as the histiocytic totipotency of object and Plant Tissue Breeding can be completed in 80~120d from explant stem tuber
Bud point to intact plant a cycle, greatly accelerate wrinkle leaf Alpinia japonica reproduction speed.
(2) wrinkle leaf Alpinia japonica Wild ornamental resources can be preserved, wrinkle leaf Alpinia japonica seedling stability is improved, passes through the bud point on stem tuber
The seedling of vegetative propagation can keep the hereditary information of parent, maternal fine quality to be stabilized heredity completely.The kind of proliferation
Seedling has the advantages that robust seedling, growth are consistent, convenient for unified management, saves production cost.
(3) by induction and Multiplying culture, the breeding coefficient of wrinkle leaf Alpinia japonica is substantially increased；Culture of rootage obtains blade and stretches
It opens up, the strong sprout of well developed root system, enhances the resistance of seedling, improve cultivation survival rate.
The wrinkle leaf Alpinia japonica tissue cultivation rapid breeding method of the present invention is simple and easy to do, can in a short time obtain largely without strain
Seedling, plant strain growth is healthy and strong, and input low output is high, is conducive to mass market and promotes.
Description of the drawings
Fig. 1 is that the wrinkle leaf Alpinia japonica of embodiment 1 peels off the explant after crust disinfection.
Fig. 2 is the adventitious bud that 1 explant of embodiment accesses adventitious bud induction culture base 12d.
Fig. 3 is the Multiple Buds that embodiment 1 cuts that adventitious bud accesses adventitious bud proliferation culture medium 20d.
Fig. 4 is the rooted seedling intact plant that 1 Rooting and hardening-off culture of embodiment is formed.
Fig. 5 is that embodiment 1 cleans the rooted seedling after the culture medium disinfection of root.
Fig. 6 is the wrinkle leaf Alpinia japonica seedling of 1 transplant survival of embodiment.
Specific implementation mode
The following examples are further illustrations of the invention, rather than limiting the invention.Do not have in following instance
The dated experimental method of body, can conventionally carry out, or according to the operation instruction of production manufacturer used；It is used
Material, reagent etc., unless otherwise specified, can be obtained by commercial sources.
Embodiment 1
The embodiment of the present invention provides a kind of tissue cultivation rapid breeding method of wrinkle leaf Alpinia japonica comprising following steps：
(1) explant sterilizes：The wrinkle leaf Alpinia japonica subterranean stem for selecting sprouting, is wiped clean with paper handkerchief, cuts off the palpus on stem tuber
Root cuts stem apex, peels off outer layer covers blade, and soil is rinsed by explant under 75% ethanol water of volume fraction and is done
Only.The stem tuber that cutting contains single bud point is explant, and is carried out disinfection to explant；75% ethanol water of volume fraction disappears
Malicious 20s, sterile water wash 1 time；0.1% mercuric chloride of mass fraction is used to sterilize 10min, aseptic water washing 4 times again；Explant after disinfection
Body is as shown in Figure 1.
(2) adventitious bud inducing：The bud point explant disinfected in step (1) is inoculated into adventitious bud induction culture base,
The Fiber differentiation of adventitious bud is carried out, explant 12d on inducing culture forms the adventitious bud (as shown in Figure 2) of high 1~2cm；
The adventitious bud induction culture base：Every liter contains 6- benzyl aminoadenines 2.0mg, niacin 0.2mg, sucrose 20g, agar
5.0g, surplus are MS culture mediums, pH value 5.6；Adventitious bud induction culture condition is：27 ± 2 DEG C of temperature, intensity of illumination
1800lx, light application time 10h/d, adventitious bud induction culture time are 28d.
(3) adventitious bud proliferation：The adventitious bud induced in step (2) is cut, blade and stem are cut away, stays bastem portion, is inoculated with
To squamous subculture is carried out in adventitious bud proliferation culture medium, the adventitious bud of cutting cultivates 20d on adventitious bud proliferation culture medium can
Form 3-5 Multiple Buds (as shown in Figure 3)；The adventitious bud proliferation culture medium：Every liter containing 6- benzyl aminoadenines 1.5mg,
Niacin 0.1mg, sucrose 20g, agar 5g, surplus are MS culture mediums, pH value 5.6.Adventitious bud proliferation condition of culture is：Temperature
27 ± 2 DEG C, intensity of illumination 1800lx, light application time 10h/d, the time of Multiplying culture is 40d.
(4) strong plantlets and rootage：The single Multiple Buds of 4~6cm of height of seedling in step (3) are cut, and are inoculated into strong plantlets and rootage
Culture of rootage is carried out in culture medium, to form the rooted seedling (as shown in Figure 4) of intact plant；The Rooting and hardening-off culture base：Often
It rises containing niacin 0.3mg, sucrose 20g, agar 5g, surplus is MS culture mediums, pH value 5.6.The condition of Rooting and hardening-off culture
For：The time of 27 ± 2 DEG C, intensity of illumination 1800lx, light application time 10h/d of temperature, Rooting and hardening-off culture is 26d.
(5) acclimatization and transplants：By the rooted seedling of the high 6~8cm of culture in step (4), root culture medium is cleaned (such as Fig. 5 institutes
Show), in the transplantation of seedlings to cultivation matrix that will take root after sterilized, normal training orientation can be obtained wrinkle leaf Alpinia japonica seedling (such as Fig. 6 institutes
Show).The cultivation matrix is by fine sand, perlite and peat soil with weight ratio 1：2：3 mixtures being uniformly mixed so as to obtain；The disinfection
It is that 0.1% Bravo solution of use quality score impregnates 30min；It takes root after transplantation of seedlings to cultivation matrix, carries out shading culture, train
Support 23 DEG C of temperature, relative air humidity 70%.
Embodiment 2
The embodiment of the present invention provides a kind of tissue cultivation rapid breeding method of wrinkle leaf Alpinia japonica comprising following steps：
(1) explant sterilizes：The wrinkle leaf Alpinia japonica subterranean stem for selecting sprouting, is wiped clean with paper handkerchief, cuts off the palpus on stem tuber
Root cuts stem apex, peels off outer layer covers blade, and explant is rushed soil under 75% ethanol water of volume fraction of flowing
Wash clean.The stem tuber that cutting contains single bud point is explant, and is carried out disinfection to explant；75% ethyl alcohol of volume fraction is water-soluble
Liquid disinfectant 25s, sterile water wash 1 time；0.1% mercuric chloride of mass fraction is used to sterilize 9min, aseptic water washing 4 times again.
(2) adventitious bud inducing：The bud point explant disinfected in step (1) is inoculated into adventitious bud induction culture base,
Carry out the Fiber differentiation of adventitious bud；The adventitious bud induction culture base：Every liter contains 6- benzyl aminoadenines 3.0mg, niacin
0.3mg, sucrose 30g, agar 5.5g, surplus are MS culture mediums, pH value 5.8；Adventitious bud induction culture condition is：Temperature 27 ±
2 DEG C, intensity of illumination 2000lx, light application time 12h/d, the adventitious bud induction culture time is 25d.
(3) adventitious bud proliferation：The adventitious bud induced in step (2) is cut, blade and stem are cut away, stays bastem portion, is inoculated with
To carrying out squamous subculture in adventitious bud proliferation culture medium；The adventitious bud proliferation culture medium：Every liter contains 6- benzyl aminoadenines
2.0mg, niacin 0.15mg, sucrose 25g, agar 5.5g, surplus are MS culture mediums, pH value 5.8.Adventitious bud proliferation culture item
Part is：The time of 27 ± 2 DEG C, intensity of illumination 2000lx, light application time 12h/d of cultivation temperature, Multiplying culture is 35d.
(4) strong plantlets and rootage：The single Multiple Buds of 4~6cm of height of seedling in step (3) are cut, and are inoculated into strong plantlets and rootage
Culture of rootage is carried out in culture medium, to form the rooted seedling of intact plant；The Rooting and hardening-off culture base：Every liter contains niacin
0.5mg, sucrose 25g, agar 5.5g, surplus are MS culture mediums, pH value 5.8.The condition of Rooting and hardening-off culture is：Temperature 27 ±
2 DEG C, intensity of illumination 2000lx, light application time 12h/d, the time of Rooting and hardening-off culture is 20d.
(5) acclimatization and transplants：By the rooted seedling of the high 6~8cm of culture in step (4), root culture medium is cleaned, it will after sterilized
It takes root in transplantation of seedlings to cultivation matrix, normal training orientation can be obtained wrinkle leaf Alpinia japonica seedling.The cultivation matrix be by fine sand,
Perlite and peat soil are with weight ratio 2：1：4 mixtures being uniformly mixed so as to obtain；The disinfection is 0.2% Bravo of use quality score
Solution impregnates 35min；It takes root after transplantation of seedlings to cultivation matrix, carries out shading culture, 25 DEG C of cultivation temperature, relative air humidity
80%.
Embodiment 3
The embodiment of the present invention provides a kind of tissue cultivation rapid breeding method of wrinkle leaf Alpinia japonica comprising following steps：
(1) explant sterilizes：The wrinkle leaf Alpinia japonica subterranean stem for selecting sprouting, is wiped clean with paper handkerchief, cuts off the palpus on stem tuber
Root cuts stem apex, peels off outer layer covers blade, and explant is rushed soil under 75% ethanol water of volume fraction of flowing
Wash clean.The stem tuber that cutting contains single bud point is explant, and is carried out disinfection to explant；75% ethyl alcohol of volume fraction is water-soluble
Liquid disinfectant 30s, sterile water wash 1 time；0.1% mercuric chloride of mass fraction is used to sterilize 8min, aseptic water washing 6 times again.
(2) adventitious bud inducing：The bud point explant disinfected in step (1) is inoculated into adventitious bud induction culture base,
Carry out the Fiber differentiation of adventitious bud；The adventitious bud induction culture base：Every liter contains 6- benzyl aminoadenines 3.5mg, niacin
0.3mg, sucrose 30g, agar 6.0g, surplus are MS culture mediums, pH value 5.6；Adventitious bud induction culture condition is：Temperature 27 ±
2 DEG C, intensity of illumination 2300lx, light application time 14h/d, the adventitious bud induction culture time is 35d.
(3) adventitious bud proliferation：The adventitious bud induced in step (2) is cut, blade and stem are cut away, stays bastem portion, is inoculated with
To carrying out squamous subculture in adventitious bud proliferation culture medium；The adventitious bud proliferation culture medium：Every liter contains 6- benzyl aminoadenines
2.5mg, niacin 0.18mg, sucrose 30g, agar 6g, surplus are MS culture mediums, pH value 5.8.Adventitious bud proliferation condition of culture
For：The time of 27 ± 2 DEG C, intensity of illumination 2300lx, light application time 14h/d of cultivation temperature, Multiplying culture is 45d.
(4) strong plantlets and rootage：The single Multiple Buds of 4~6cm of height of seedling in step (3) are cut, and are inoculated into strong plantlets and rootage
Culture of rootage is carried out in culture medium, to form the rooted seedling of intact plant；The Rooting and hardening-off culture base：Every liter contains niacin
0.8mg, sucrose 30g, agar 6g, surplus are MS culture mediums, pH value 5.8.The condition of Rooting and hardening-off culture is：Temperature 27 ± 2
DEG C, the time of intensity of illumination 2300lx, light application time 14h/d, Rooting and hardening-off culture are 30d.
(5) acclimatization and transplants：By the rooted seedling of the high 6~8cm of culture in step (4), root culture medium is cleaned, it will after sterilized
It takes root in transplantation of seedlings to cultivation matrix, normal training orientation can be obtained wrinkle leaf Alpinia japonica seedling.The cultivation matrix be by fine sand,
Perlite and peat soil are with weight ratio 2：2：3 mixtures being uniformly mixed so as to obtain；The disinfection is 0.3% Bravo of use quality score
Solution impregnates 40min；It takes root after transplantation of seedlings to cultivation matrix, carries out shading culture, 27 DEG C of cultivation temperature, relative air humidity is
75%.
Claims (10)
1. a kind of tissue cultivation rapid breeding method of wrinkle leaf Alpinia japonica, which is characterized in that include the following steps：
A. explant sterilizes：The wrinkle leaf Alpinia japonica subterranean stem of sprouting is selected, the blade of fibrous root, stem apex, outer layer covers, cleaning are removed
Disinfection, cutting contain the stem tuber of single bud point as explant, carry out disinfection to explant；
B. adventitious bud inducing：Explant is inoculated into adventitious bud induction culture base, Fiber differentiation adventitious bud；The adventitious bud
Inducing culture：Every liter contains 2.0~3.5mg of 6- benzyl aminoadenines, 0.2~0.3mg of niacin, 20~30g of sucrose, agar
5.0~6.0g, surplus are MS culture mediums, and pH value is 5.6~5.8；
C. adventitious bud proliferation：Adventitious bud is cut, blade and stem is removed, stays bastem portion, be inoculated into adventitious bud proliferation culture medium
In, culture forms Multiple Buds；The adventitious bud proliferation culture medium：Every liter containing 1.5~3.0mg of 6- benzyl aminoadenines, how
0.1~0.3mg of acetic acid, 20~30g of sucrose, 5~6g of agar, surplus are MS culture mediums, and pH value is 5.6~5.8；
D. strong plantlets and rootage：Single Multiple Buds are cut, are inoculated into Rooting and hardening-off culture base, culture forms rooted seedling；It is described
Rooting and hardening-off culture base：Every liter is cultivated containing 0.3~0.8mg of niacin, 20~30g of sucrose, 5~6g of agar, surplus for MS
Base, pH value are 5.6~5.8；
E. acclimatization and transplants：Rooted seedling is taken out, root culture medium is cleaned, in the transplantation of seedlings to cultivation matrix that will take root after sterilized,
Training orientation is carried out, wrinkle leaf Alpinia japonica seedling is obtained.
2. according to the method described in claim 1, it is characterized in that, the cleaning disinfection of the step a is to use volume fraction
75% ethanol water is rinsed well；Carrying out disinfection to explant for the step a is by explant volume fraction 75%
Ethanol water impregnates 20~30s, sterile water wash 1 time；Again use 0.08%~0.12% mercuric chloride of mass fraction impregnate 8~
10min, aseptic water washing 4~6 times.
3. according to the method described in claim 1, it is characterized in that, the condition of culture in described step b, c, d is：Temperature 27
± 2 DEG C, 1800~2300lx of intensity of illumination, 10~14h/d of light application time.
4. according to the method described in claim 3, it is characterized in that, the condition of culture in described described step b, c, the d is：
27 ± 2 DEG C, intensity of illumination 2000lx, light application time 12h/d of temperature.
5. according to the method described in claim 1, it is characterized in that, the disinfection of the step e is by rooted seedling use quality
0.1%~0.3% Bravo solution of score impregnates 30~40min.
6. according to the method described in claim 1, it is characterized in that, the cultivation matrix of the step e is by fine sand, perlite
With peat soil with weight ratio 1~2：1~2：3~4 mixtures being uniformly mixed so as to obtain.
7. according to the method described in claim 1, it is characterized in that, the condition of culture of the step e is：Temperature 23~27
DEG C, relative air humidity 70%~80%.
8. according to the method described in claim 1, it is characterized in that, the adventitious bud induction culture base：Every liter contains 6- benzyls
Aminoadenine 3.0mg, niacin 0.3mg, sucrose 30g, agar 5.5g, surplus are MS culture mediums, pH value 5.8.
9. according to the method described in claim 1, it is characterized in that, the adventitious bud proliferation culture medium：Every liter contains 6- benzyls
Aminoadenine 2.0mg, niacin 0.15mg, sucrose 25g, agar 5.5g, surplus are MS culture mediums, pH value 5.8.
10. according to the method described in claim 1, it is characterized in that, the Rooting and hardening-off culture base：Every liter contains niacin
0.5mg, sucrose 25g, agar 5.5g, surplus are MS culture mediums, pH value 5.8.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114568307A (en) * | 2022-03-24 | 2022-06-03 | 中国热带农业科学院热带作物品种资源研究所 | Method for rapidly breeding seedlings by utilizing alpinia katsumadai stem tips |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107667860A (en) * | 2017-10-18 | 2018-02-09 | 广州普邦园林股份有限公司 | Tissue culture propagation method of alpinia japonica |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107667860A (en) * | 2017-10-18 | 2018-02-09 | 广州普邦园林股份有限公司 | Tissue culture propagation method of alpinia japonica |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114568307A (en) * | 2022-03-24 | 2022-06-03 | 中国热带农业科学院热带作物品种资源研究所 | Method for rapidly breeding seedlings by utilizing alpinia katsumadai stem tips |
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