CN108663527A - Blood clotting and hemagglutination-inhibition test readout instrument - Google Patents
Blood clotting and hemagglutination-inhibition test readout instrument Download PDFInfo
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- CN108663527A CN108663527A CN201710200048.0A CN201710200048A CN108663527A CN 108663527 A CN108663527 A CN 108663527A CN 201710200048 A CN201710200048 A CN 201710200048A CN 108663527 A CN108663527 A CN 108663527A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
Abstract
A kind of blood clotting of present invention offer and hemagglutination-inhibition test result automatic interpretation tool, the equipment for automatically generating result.Include the following steps：Blood clotting board mount adjusts, and red blood cell is made to form teardrop shaped first；Then subregion is carried out to blood-coagulation-board sample well to take pictures, calculate the tear length of red blood cell, find out target hole in fixture away from；Original image and sentence read result are stored in database, while will carry out statistic of classification according to the test result of sample and detection project by final result processing, are summarized to sample, by clicking menu, are exported result.Blood clotting proposed by the present invention and hemagglutination test interpretation device and statistical method, once can 96 samples of interpretation, only need 4 second time, interpretation speed than manually it is faster, it is time saving and energy saving.Furthermore with the present invention without manually analysis discrimination, visual fatigue and erroneous judgement are avoided, individual difference is avoided；Human contact's cause of disease chance is reduced simultaneously, while can manage system docking with sample message, realizes automatic management.
Description
Technical field
The invention belongs to a kind of field of biological detection, and in particular to a kind of blood clotting and hemagglutination-inhibition test result are sentenced automatically
It reads, automatically generates the method and apparatus of result.
Background technology
Blood clotting and hemagglutination-inhibition test are mainly for detection of Serum Antibody level, it can also be used to judge the infection shape of group
State etc..The experiment at present is that the World Health Organization and the global bird flu of international animal health tissues progress, newcastle disease etc. are great dynamic
Object epidemic disease detects the method generally used, and because its is easy to operate, can handle the advantages that a large amount of sample, has become
The most commonly used method in current base layer veterinary laboratories Disease monitor and immune antiboidy detection.
During blood clotting and hemagglutination-inhibition test, according to the power of red blood cell agglutinating reaction on blood-coagulation-board, it usually needs
Carry out 2 artificial interpretations.Firstly the need of the potency of artificial interpretation hemagglutination test, 4 unit antigens are configured according to potency, are then carried out
Hemagglutination-inhibition test carries out artificial interpretation again, finally determines test result.According to sentence read result, it is carried out at the same time manual record.
There are the following problems for existing result interpretation：1. artificial interpretation, is affected by artificial subjective factor, will produce sometimes
Visual fatigue be easy to cause result error.2. since what is usually detected is all pathogenic microorganism, excessive and human contact deposits
In bio-safety hidden danger.3. artificial interpretation and record, time-consuming and laborious.4. artificial interpretation can not with sample management information system from
Dynamic docking and the automatic management for realizing test result.
In view of this, nowadays there is an urgent need to design a kind of new blood clotting and hemagglutination test interpretative instrument, it is existing to overcome
Drawbacks described above existing for interpretation.
Invention content
The technical problem to be solved by the present invention is to：A kind of interpretation blood clotting and hemagglutination-inhibition test are provided as a result, simultaneously automatic
The method and apparatus of record and statistical results.
In order to solve the above technical problems, the present invention adopts the following technical scheme that：
On the one hand the present invention provides a kind of hemagglutination test result interpretation device, it is characterised in that including with lower component：
(1) blood clotting board mount for fixing and accommodating blood-coagulation-board, and makes blood-coagulation-board plane with respect to the horizontal plane generate folder
Angle；
(2) image acquiring device, the fluoroscopy images for obtaining the sample well on blood-coagulation-board；
(3) image conversion apparatus, for the image in each hole to be converted into HSL formats by rgb format；
(4) result computing device carries out image cutting for the HSL images to each hole, calculates erythrocyte agglutination pattern
Length, the result of hemagglutination test is determined according to the length of erythrocyte agglutination pattern；
(5) storage device, the storage device can store the original image of acquisition and the sentence read result per hole；
(6) result output device, the result output device include display and/or printer.
Hemagglutination test result interpretation device of the present invention, it is characterised in that the blood clotting board mount can be adjusted
Save to change the angle of blood-coagulation-board plane with respect to the horizontal plane angle, the included angle is between 1-90 degree.
Hemagglutination test result interpretation device of the present invention, it is characterised in that the image acquiring device be 1 or
Multiple digital cameras.
Hemagglutination test result interpretation device of the present invention, it is characterised in that described image conversion equipment can be by RGB
Image carries out binaryzation with Hue 15 and is converted to corresponding HSL images.
Hemagglutination test result interpretation device of the present invention, it is characterised in that the result computing device determines red blood cell
The largest contours of pattern are aggregated, obtain the corresponding minimum area-encasing rectangle of the profile, the long side of rectangle is erythrocyte agglutination pattern
Length.
Hemagglutination test result interpretation device of the present invention, it is characterised in that the result computing device, which first calculates, often goes
The length of erythrocyte agglutination pattern in blood clotting hole takes the average value for being aggregated maximum 3 holes of pattern length in every row to be denoted as L1；Then
Determine that blood clotting hole of first erythrocyte agglutination pattern length less than 75%L1 is as target hole in the row, according to the dilute of target hole
Degree of releasing judges hemagglutinative titer.
Hemagglutination test result interpretation device of the present invention, it is characterised in that the storage device includes hard disk, light
Disk, and/or network memory.
Second aspect, the present invention provide the hemagglutination test result interpretation device and sentence in blood clotting, hemagglutination-inhibition test result
Application in fixed.
The third aspect, the present invention provide a kind of method of interpretation hemagglutination test result, including：
(1) blood-coagulation-board is fixed on holder and blood-coagulation-board plane is made with respect to the horizontal plane to generate angle；
(2) fluoroscopy images of each sample well on blood-coagulation-board are obtained；
(3) image in each hole is converted into HSL formats by rgb format；
(4) image cutting is carried out to the HSL images in each hole, the length of erythrocyte agglutination pattern is calculated, according to red blood cell
The length of agglutination pattern determines the result of hemagglutination test；
(5) test result is stored
(6) output test result.
The method of the invention, wherein step (4) include
(a) largest contours for determining erythrocyte agglutination pattern obtain the corresponding minimum area-encasing rectangle of the profile, by rectangle
Length of the long side as erythrocyte agglutination pattern；
(b) length for coagulating erythrocyte agglutination pattern in hole per promoting circulation of blood is calculated, takes and is aggregated maximum 3 holes of pattern length in every row
Average value be denoted as L1；
(c) it is less than the blood clotting hole of row L1 × 75% using first erythrocyte agglutination pattern length in every row as target
Hole, according to target.
The technical solution of invention is described in detail below.
The present invention provides a kind of hemagglutination test result interpretation device, and automatically records the method with statistical results, institute
The method of stating includes the following steps：
Step S1,96 hole blood clotting board mount；
96 hole blood-coagulation-boards of experiment (Fig. 1-1-2) are placed on the rectangular support frame that one matches with blood-coagulation-board, according to
Test result needs to adjust blood-coagulation-board angle, and the red blood cell in blood-coagulation-board sample well is made to form teardrop shaped under the action of oblique force.
Step S2, the interpretation of hemagglutination test result；
Startup program first carries out subregion to the sample well on blood-coagulation-board away from in fixture by 4 cameras and takes pictures；Due to
The position in each hole is fixed, and after taking pictures, system carries out image cutting according to fixed pixel position to each hole, is calculated red thin
The tear length (Fig. 2) of born of the same parents.Sample red blood cell to be analyzed is red tone in hemagglutination test result, and system is by picture from RGB
Format conversion is at HSL formats, and according to the sample state taken, statistics, which obtains its colour system Hue, should be less than 15, can obtain
Corresponding binary map (Fig. 3).By finding its largest contours, the corresponding minimum area-encasing rectangle of profile is found out, long side is blood
Tear length.Then row statistics is pressed, calculates separately out the length of 12 samples of every row, finding out maximum 3 holes of length, (hole count is not
Foot is calculated by practical hole number), its average tear length value L 1 (Fig. 4) is calculated, while being looked for from right to left successively in one's own profession
Go out first tear hole for being less than 75% length of L1, the requirement of correlation test standard is judged according to hemagglutination test, this hole left side
Hole is target hole, is exactly Coupon Testing Results according to the hemagglutinative titer value of this hole interpretation.
Step S3, the statistics of hemagglutination test result；
The sentence read result that system is gone by the original image of test result and often is stored in database, while will be according to sample and inspection
The items test result such as survey project carries out statistic of classification, and sample can also be marked.Project is such as examined by clicking menu,
System collect statistics on fixed table automatically record sentence read result；Test sample can also be numbered and be re-flagged,
It marks and changes directly on EXCELL tables；As a result, instant look into, click output menu can also be beaten food preservation test simultaneously
Print test result.
A kind of hemagglutination-inhibition test result interpretation device, and automatically record the method with statistical results, the method
Include the following steps：
Step S1,96 hole blood clotting board mount；
96 blood-coagulation-boards of experiment (Fig. 1-1-2) are placed on the rectangular support frame that one matches with blood-coagulation-board, according to examination
It tests result to need to adjust blood-coagulation-board angle, the red blood cell in blood-coagulation-board sample well is made to form teardrop shaped under the action of oblique force.
Step S2, the interpretation of hemagglutination-inhibition test result；
Startup program first carries out subregion to the sample well on blood-coagulation-board away from in fixture by 4 cameras and takes pictures；Due to
The position in each hole is fixed, and after taking pictures, system carries out image cutting according to fixed pixel position to each hole, is calculated red thin
The tear length (Fig. 2) of born of the same parents.Since sample red blood cell to be analyzed in hemagglutination test result is red tone, system by picture from
Rgb format is converted into HSL formats, and according to the sample state taken, statistics, which obtains its colour system Hue, should be less than 15, can be with
Obtain corresponding binary map (Fig. 3).By finding its largest contours, the corresponding minimum area-encasing rectangle of profile is found out, long side is
Blood tear length.Then it presses row to count, after the length for calculating separately out 12 samples of every row, finds out maximum 3 holes of length
(hole count is insufficient to be calculated by practical hole number), calculates its average tear length value L 1 (Fig. 4).Simultaneously successively from a left side in one's own profession
First tear hole for being less than 75% length of L1 is found out to the right side, the requirement of correlation test standard, this hole are judged according to hemagglutination test
The hole on the left side is target hole, is exactly Coupon Testing Results according to the hemagglutinative titer value of this hole interpretation.
Step S3, the statistics of hemagglutination-inhibition test result；
The sentence read result that system is gone by the original image of test result and often is stored in database, while will be according to sample and inspection
The items test result such as survey project carries out statistic of classification, and sample can also be marked.Project is such as examined by clicking menu,
System collect statistics on fixed table automatically record sentence read result；Test sample can also be numbered and be re-flagged,
It marks and changes directly on EXCELL tables；As a result, instant look into, click output menu can also be beaten food preservation test simultaneously
Print test result.
Further include that can be supported and solid there are one rectangular support frame in step S1 as a preferred embodiment of the present invention
Fixed 96 hole blood-coagulation-boards, and the angle for placing blood clotting board mount can be adjusted according to test situation, not need manual control,
Save manpower.
As a preferred embodiment of the present invention, in step S2, which is based on taking pictures and calculating, and there have to be very strong accurate
Property.When shooting entire microwell plate with a camera, the distortion of its edge is particularly evident, and the present invention is solved using 4 cameras
This problem, every camera are responsible for 1/4 region therein, and four cameras are simultaneously taken pictures to target blood-coagulation-board, will be photographed
Sample counted, calculate red blood cell tear length, target hole, i.e. test result interpretation hole are selected according to tear length, speed
Degree is exceedingly fast.
As a preferred embodiment of the present invention, in step S3, which is based on statistics and summarizes, with portion serum sample
Product, it is sometimes desirable to the detection of multiple projects is carried out, it can be by clicking target menu, system automatically will will be same according to sample number into spectrum
A variety of project test results of a sample are summarised on same table, carry out statistical analysis, time saving province convenient for check experiment result
Power, it is convenient and efficient.
The beneficial effects of the present invention are：Blood clotting proposed by the present invention and hemagglutination test interpretation device and test result statistics
Method can once shoot 96 samples using 4 cameras, it is only necessary to 4 second time, and artificial interpretation, for knack
For personnel, 2 minutes or so are also wanted, therefore interpretation speed is more faster than artificial interpretation, it is time saving and energy saving.Furthermore with nothing of the present invention
Analysis discrimination need to be carried out to single hole with human eye, avoid visual fatigue and erroneous judgement, avoid the individual difference of artificial judgment；The present invention subtracts
Few human contact's cause of disease chance, inanimate object security risk；The present invention can manage the automatic butt of system with sample message, realize
The automatic management of test result.Each sample has picture, if encountering interference (strong light, label, foreign matter block) operator
It is easy to find；In addition, blood clotting board mount can be adjusted manually, it is easy to use.
Description of the drawings
Fig. 1 is interpretation equipment body structure in the method for the present invention.
Fig. 2 is automatic interpretation flow chart in the method for the present invention.
Fig. 3 is image cutting schematic diagram in the method for the present invention.
Fig. 4 is that the method for the present invention target hole calculates schematic diagram.
Fig. 5 is that actually photographed hemagglutination test photo in the method for the present invention.
Fig. 6 is that actually photographed hemagglutination-inhibition test photo in the method for the present invention.
Specific implementation mode
The preferred embodiment that the invention will now be described in detail with reference to the accompanying drawings.
Embodiment one
Referring to Fig. 5, present invention is disclosed a kind of hemagglutination test result interpretation devices, and automatically record and statistical test knot
The method of fruit, described method includes following steps：
【Step S1】96 hole blood clotting board mounts；
96 hole blood-coagulation-boards of experiment are placed on the rectangular support frame that one matches with blood-coagulation-board, according to test result need
Blood-coagulation-board angle is adjusted, the red blood cell in blood-coagulation-board sample well is made to form teardrop shaped under the action of oblique force.
【Step S2】The interpretation of hemagglutination test result；
Startup program first carries out subregion to the sample well on blood-coagulation-board away from in fixture by 4 cameras and takes pictures；Due to
The position in each hole is fixed, and after taking pictures, system carries out image cutting according to fixed pixel position to each hole, is calculated red thin
The tear length (Fig. 2) of born of the same parents.Sample red blood cell to be analyzed is red tone in hemagglutination test result, and system is by picture from RGB
Format conversion is at HSL formats, and according to the sample state taken, statistics, which obtains its colour system Hue, should be less than 15, can obtain
Corresponding binary map (Fig. 3).By finding its largest contours, the corresponding minimum area-encasing rectangle of profile is found out, long side is blood
Tear length.Then press row statistics, calculate separately out the length of 12 samples of every row, find out maximum 3 holes of length (the 10th,
11,12 hole), its average tear length value is calculated, while finding out first from right to left successively in one's own profession and being less than the length of L1 75%
The tear hole of degree i.e. the 9th hole judges the requirement of correlation test standard according to hemagglutination test, and the 8th hole of hole on this hole left side is mesh
Hole is marked, the hemagglutinative titer value according to this hole interpretation is exactly judgement result i.e. 2 of sampling test8.According to same method, the second row
Judge that result is 210, the judgement result of the third line is also 210。
【Step S3】The statistics of hemagglutination test result；
The sentence read result that system is gone by the original image of test result and often is stored in database, while will be according to sample and inspection
The items test result such as survey project carries out statistic of classification, and sample can also be marked.Project is such as examined by clicking menu,
System collect statistics on fixed table automatically record sentence read result；Test sample can also be numbered and be re-flagged,
It marks and changes directly on EXCELL tables；As a result, instant look into, click output menu can also be beaten food preservation test simultaneously
Print test result.
Embodiment two
Referring to Fig. 6, present invention is disclosed a kind of hemagglutination-inhibition test result interpretation devices, and automatically record and count examination
The method for testing result, described method includes following steps：
【Step S1】96 hole blood clotting board mounts；
96 hole blood-coagulation-boards of experiment are placed on the rectangular support frame that one matches with blood-coagulation-board, according to test result need
Blood-coagulation-board angle is adjusted, the red blood cell in blood-coagulation-board sample well is made to form teardrop shaped under the action of oblique force.
【Step S2】The interpretation of hemagglutination-inhibition test result；
Startup program first carries out subregion to the sample well on blood-coagulation-board away from in fixture by 4 cameras and takes pictures；Due to
The position in each hole is fixed, and after taking pictures, system carries out image cutting according to fixed pixel position to each hole, is calculated red thin
The tear length (Fig. 2) of born of the same parents.Since sample red blood cell to be analyzed in hemagglutination test result is red tone, system by picture from
Rgb format is converted into HSL formats, and according to the sample state taken, statistics, which obtains its colour system Hue, should be less than 15, can be with
Obtain corresponding binary map (Fig. 3).By finding its largest contours, the corresponding minimum area-encasing rectangle of profile is found out, long side is
Blood tear length.Then it presses row to count, after the length for calculating separately out 12 samples of every row, finds out maximum 3 holes of length
(hole count is insufficient to be calculated by practical hole number), calculates its average tear length value L 1 (Fig. 4).Simultaneously successively from a left side in one's own profession
First tear hole i.e. the 9th hole for being less than 75% length of L1 is found out to the right side, judges wanting for correlation test standard according to hemagglutination test
It asks, the hole on this hole left side is the 8th hole of target hole, and it is exactly the judgement of sampling test to inhibit valence value according to the blood clotting of this hole interpretation
As a result it is 28.According to same method, the judgement result of the second row is 27, the judgement result of the third line is also 27。
【Step S3】The statistics of hemagglutination-inhibition test result；
The sentence read result that system is gone by the original image of test result and often is stored in database, while will be according to sample and inspection
The items test result such as survey project carries out statistic of classification, and sample can also be marked.Project is such as examined by clicking menu,
System collect statistics on fixed table automatically record sentence read result；Test sample can also be numbered and be re-flagged,
It marks and changes directly on EXCELL tables；As a result, instant look into, click output menu can also be beaten food preservation test simultaneously
Print test result.
In conclusion blood clotting proposed by the present invention and hemagglutination test interpretation equipment, realize whole-course automation, utilize 4 photographs
Camera can once shoot 96 samples, it is only necessary to 4 second time, and artificial interpretation also wants 2 for the skilled person
Minute or so, therefore interpretation speed is more much faster than artificial interpretation, it is time saving and energy saving.It is not necessarily to human eye to single hole furthermore with the present invention
Analysis discrimination is carried out, visual fatigue and erroneous judgement are avoided, avoids artificial judgment individual difference；The present invention reduces human contact's cause of disease
Chance, inanimate object security risk；The present invention can manage the automatic butt of system with sample message, realize the automatic of test result
Change management.Each sample has picture, is easy to find if encountering interference (strong light, label, foreign matter block) operator；In addition,
Blood clotting holder can be adjusted manually, easy to use.
Description and application of the invention herein are illustrative, is not wishing to limit the scope of the invention to above-described embodiment
In.The deformation and change of embodiments disclosed herein are possible, real for those skilled in the art
The replacement and equivalent various parts for applying example are well known.It should be appreciated by the person skilled in the art that not departing from the present invention
Spirit or essential characteristics in the case of, the present invention can in other forms, structure, arrangement, ratio, and with other components,
Material and component are realized.Without departing from the scope and spirit of the present invention, can to embodiments disclosed herein into
The other deformations of row and change.
Claims (10)
1. a kind of hemagglutination test result interpretation device, it is characterised in that including with lower component：
(1) blood clotting board mount for fixing and accommodating blood-coagulation-board, and makes blood-coagulation-board plane with respect to the horizontal plane generate angle；
(2) image acquiring device, the fluoroscopy images for obtaining the sample well on blood-coagulation-board；
(3) image conversion apparatus, for the image in each hole to be converted into HSL formats by rgb format；
(4) result computing device carries out image cutting for the HSL images to each hole, calculates the length of erythrocyte agglutination pattern
Degree, the result of hemagglutination test is determined according to the length of erythrocyte agglutination pattern；
(5) storage device, the storage device can store the original image of acquisition and the sentence read result per hole；
(6) result output device, the result output device include display and/or printer.
2. hemagglutination test result interpretation device as described in claim 1, it is characterised in that the blood clotting board mount can be into
Row adjusts to change the angle of blood-coagulation-board plane with respect to the horizontal plane angle, and the included angle is between 1-90 degree.
3. hemagglutination test result interpretation device as described in claim 1, it is characterised in that the image acquiring device is 1
Or multiple digital cameras.
4. hemagglutination test result interpretation device as described in claim 1, it is characterised in that described image conversion equipment can incite somebody to action
RGB image carries out binaryzation with Hue 15 and is converted to corresponding HSL images.
5. hemagglutination test result interpretation device as described in claim 1, it is characterised in that the result computing device determines red
The largest contours of cell agglutination pattern obtain the corresponding minimum area-encasing rectangle of the profile, and the long side of rectangle is erythrocyte agglutination
The length of pattern.
6. the hemagglutination test result interpretation device as described in claim 1 or 5, it is characterised in that the result computing device is first counted
The length for coagulating erythrocyte agglutination pattern in hole per promoting circulation of blood is calculated, the average value for being aggregated maximum 3 holes of pattern length in every row is taken to be denoted as
L1；Then determine that blood clotting hole of first erythrocyte agglutination pattern length less than 75%L1 is as target hole in the row, according to mesh
The dilution in mark hole judges hemagglutinative titer.
7. hemagglutination test result interpretation device as described in claim 1, it is characterised in that the storage device include hard disk,
CD, and/or network memory.
8. hemagglutination test result interpretation device the answering in blood clotting, hemagglutination-inhibition test result judgement as described in claim 1-7
With.
9. a kind of method of interpretation hemagglutination test result, including：
(1) blood-coagulation-board is fixed on holder and blood-coagulation-board plane is made with respect to the horizontal plane to generate angle；
(2) fluoroscopy images of each sample well on blood-coagulation-board are obtained；
(3) image in each hole is converted into HSL formats by rgb format；
(4) image cutting is carried out to the HSL images in each hole, the length of erythrocyte agglutination pattern is calculated, according to erythrocyte agglutination
The length of pattern determines the result of hemagglutination test；
(5) test result is stored
(6) output test result.
10. the method for interpretation hemagglutination test result as claimed in claim 9, it is characterised in that the step (4) includes
(a) largest contours for determining erythrocyte agglutination pattern obtain the corresponding minimum area-encasing rectangle of the profile, by the long side of rectangle
Length as erythrocyte agglutination pattern；
(b) length for coagulating erythrocyte agglutination pattern in hole per promoting circulation of blood is calculated, takes and is aggregated the flat of maximum 3 holes of pattern length in every row
Mean value is denoted as L1；
(c) it is less than the blood clotting hole of row L1 × 75% using first erythrocyte agglutination pattern length in every row as target hole, root
According to the hemagglutinative titer of the dilution judgement sample of target hole.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115272325A (en) * | 2022-09-28 | 2022-11-01 | 广州知力医学诊断技术有限公司 | Platelet antibody detection result interpretation device and method |
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