CN108663359A - Residues of formaldehyde assay kit in a kind of mushroom - Google Patents
Residues of formaldehyde assay kit in a kind of mushroom Download PDFInfo
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- CN108663359A CN108663359A CN201810349153.5A CN201810349153A CN108663359A CN 108663359 A CN108663359 A CN 108663359A CN 201810349153 A CN201810349153 A CN 201810349153A CN 108663359 A CN108663359 A CN 108663359A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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Abstract
The invention discloses residues of formaldehyde assay kits in a kind of mushroom, are made of supporter, color developing agent, white cloth and colorimetric card containing test agent, the test agent mainly contains following raw material：Citric acid, AHMT solution, glyceryl triacetate, lithium hydroxide and sodium ethylene diamine tetracetate.Residues of formaldehyde assay kit in mushroom of the present invention, precision is good, and detection cycle is short, strong antijamming capability, and method fast and stable is accurate, 97% or more rate of accuracy reached.And used convenient for family, detection method is simple, can voluntarily detect, and saves residues of formaldehyde testing cost.
Description
Technical field
The present invention relates to field of detection of food safety, and in particular to residues of formaldehyde assay kit in a kind of mushroom.
Background technology
Formaldehyde is the substance compared with high poison, is determined as carcinogenic and teratogen by the World Health Organization, is generally acknowledged change
One of state reaction source, and potential strong mutagen.Lot of documents is recorded, and influence of the formaldehyde to health is different in smell
Often, stimulation, allergy, abnormal pulmonary function, dysfunction of liver and immunologic dysfunction etc..After the poisoning of formaldehyde acute oral occurs
Can coup injury people oral cavity, throat, esophagus and stomach lining, while generating toxic reaction, less serious case is dizzy, cough, vomiting, upper abdomen
There is stupor, shock, pulmonary edema, hepatic renal dysfunction in pain, severe one, lead to bleeding, kidney failure and respiratory failure and death.It is long
Phase Low Level Exposure formaldehyde, can cause the damage of nervous system, immune system, respiratory system and liver, occur dizzy (pain), weary
The poisoning symptoms such as power, drowsiness, anorexia, visual impairment.
A large amount of investigation are found, contain formaldehyde in numerous food product.Formaldehyde is primarily as formation mushroom feature in mushroom
What the by-product of the enzymatic pathways of flavor generated.Content of formaldehyde in mushroom is an important indicator for weighing mushroom quality.
A large amount of dynamic variations in relation to formaldehyde are studies have shown that in the detection process of progress mushroom content of formaldehyde, mushroom
When homogenate is kept the temperature at 37 DEG C, content of formaldehyde rapid increase；The synthesis speed of 2h~2.5h formaldehyde tends towards stability；It is even after 2.5h
Content of formaldehyde reaches stable in slurries.The mushroom homogenate sample kept the temperature at 4 DEG C, variation the prolonging with the time of content of formaldehyde
Length also has increase, but amplification is little.This is because mushroom fruiting body is after being broken, suitable for formaldehyde metabolizing enzyme (γ-paddy
Aminoacyl transpeptidase and cysteinyl sulfoxide lyases) at a temperature of performance vigor, enzyme activity increases, and the enzymatic reaction of generation makes first
Aldehyde is largely metabolized generation.It therefore,, will be to final if not being controlled to enzyme activity in the pretreatment process of mushroom sample
Testing result has a huge impact.
The method of detection methanal content in mushroom mainly has spectrophotometric Colo, high performance liquid chromatography in the prior art
(HPLC) method.
Spectrophotometric Colo is spread out formaldehyde with acetylacetone,2,4-pentanedione reagent by distilling formaldehyde after being extracted in mushroom sample
Raw reaction generates the compound of yellow, and corresponding absorption value is measured under 414nm using spectrophotometer, quantitatively detects in sample
Extract obtained content of formaldehyde.
HPLC methods from mushroom sample extract formaldehyde by distilling, with DNPH (2,4-dinitrophenylhydrazine) by formaldehyde-derived at
There are the hydrazone compounds of diagnostic strong absorption in ultra-violet (UV) band, this derivative products is detached with HPLC, the purple in the channels 355nm
External detector detects, and is quantified come PARA FORMALDEHYDE PRILLS(91,95) with target peak peak area according to the chromatogram of gained.
Currently, time-consuming for formaldehyde detection reagent kit, complex steps are complicated for operation, and being unsuitable for family's use has, yet-to-be developed
Better kit is to use.
Invention content
The purpose of the present invention is to provide residues of formaldehyde assay kits in a kind of mushroom, live can fast and accurately measure
Formaldehyde in mushroom.
To achieve the above object, the present invention provides residues of formaldehyde assay kits in a kind of mushroom, by containing test agent
Supporter, color developing agent, white cloth and colorimetric card composition, the test agent mainly contain following raw material：Citric acid, AHMT solution
(Chinese is entitled：4- amino -3- hydrazines -5- dredges base -1,2,4- triazoles solution), glyceryl triacetate, lithium hydroxide and second
Sodium ethylene diamine tetracetate.
Preferably, in above-mentioned technical proposal, the test agent is counted in parts by weight, mainly contains following raw material：Citric acid
15-23 parts, 9-16 parts of AHMT solution, 4-11 parts of glyceryl triacetate, 6-17 parts of lithium hydroxide and sodium ethylene diamine tetracetate 3-9
Part.
Preferably, in above-mentioned technical proposal, the color developing agent is the ammonium ferric sulfate solution that mass concentration is 1%.
Preferably, in above-mentioned technical proposal, above support is plastic plate with holes, and test agent is placed in volume weight tube, institute
Volume weight tube is stated to be positioned in the support plate.
Preferably, in above-mentioned technical proposal, the white cloth is non-woven fabrics.
The application method of residues of formaldehyde assay kit, includes the following steps in a kind of above-mentioned mushroom：It will be in test agent
Citric acid, AHMT solution, glyceryl triacetate, lithium hydroxide, sodium ethylene diamine tetracetate and color developing agent be blended in detection pipe,
Then white cloth is immersed into mixed liquid dipping, mushroom sample to be measured is then put into detection pipe, after 5-10min, taken out
Cloth dries cloth, and the color of the color of cloth and colorimetric card is compared to the content of formaldehyde in i.e. detectable mushroom.
Compared with prior art, the present invention has the advantages that：Residues of formaldehyde assay kit in mushroom of the present invention,
Precision is good, and detection cycle is short, strong antijamming capability, and method fast and stable is accurate, 97% or more rate of accuracy reached.And it is convenient for family
It uses, detection method is simple, can voluntarily detect, and saves residues of formaldehyde testing cost.
Specific implementation mode
Present invention will be further explained below with reference to specific examples.These embodiments are merely to illustrate the present invention and do not have to
In limiting the scope of the invention.
Embodiment 1
Residues of formaldehyde assay kit in a kind of mushroom, by supporter, color developing agent, white cloth and colorimetric containing test agent
Card composition, the test agent are counted in parts by weight, mainly contain following raw material：19 parts of citric acid, 13 parts of AHMT solution, triacetic acid
4 parts of 7 parts of glyceride, 11 parts of lithium hydroxide and sodium ethylene diamine tetracetate.
The color developing agent is the ammonium ferric sulfate solution that mass concentration is 1%.
Above support is plastic plate with holes, and test agent is placed in volume weight tube, and the volume weight tube is positioned over the support
On plate.
The application method of residues of formaldehyde assay kit, includes the following steps in a kind of above-mentioned mushroom：It will be in test agent
Citric acid, AHMT solution, glyceryl triacetate, lithium hydroxide, sodium ethylene diamine tetracetate and color developing agent be blended in detection pipe,
Then white cloth is immersed into mixed liquid dipping, mushroom sample to be measured is then put into detection pipe, after 7min, take out cloth
Item dries cloth, and the color of the color of cloth and colorimetric card is compared to the content of formaldehyde in i.e. detectable mushroom.
Embodiment 2
Residues of formaldehyde assay kit in a kind of mushroom, by supporter, color developing agent, white cloth and colorimetric containing test agent
Card composition, the test agent are counted in parts by weight, mainly contain following raw material：15 parts of citric acid, 16 parts of AHMT solution, triacetic acid
9 parts of 11 parts of glyceride, 6 parts of lithium hydroxide and sodium ethylene diamine tetracetate.
The color developing agent is the ammonium ferric sulfate solution that mass concentration is 1%.
Above support is plastic plate with holes, and test agent is placed in volume weight tube, and the volume weight tube is positioned over the support
On plate.
The application method of residues of formaldehyde assay kit, includes the following steps in a kind of above-mentioned mushroom：It will be in test agent
Citric acid, AHMT solution, glyceryl triacetate, lithium hydroxide, sodium ethylene diamine tetracetate and color developing agent be blended in detection pipe,
Then white cloth is immersed into mixed liquid dipping, mushroom sample to be measured is then put into detection pipe, after 5min, take out cloth
Item dries cloth, and the color of the color of cloth and colorimetric card is compared to the content of formaldehyde in i.e. detectable mushroom.
Embodiment 3
Residues of formaldehyde assay kit in a kind of mushroom, by supporter, color developing agent, white cloth and colorimetric containing test agent
Card composition, the test agent are counted in parts by weight, mainly contain following raw material：23 parts of citric acid, 9 parts of AHMT solution, triacetic acid
3 parts of 4 parts of glyceride, 17 parts of lithium hydroxide and sodium ethylene diamine tetracetate.
The color developing agent is the ammonium ferric sulfate solution that mass concentration is 1%.
Above support is plastic plate with holes, and test agent is placed in volume weight tube, and the volume weight tube is positioned over the support
On plate.
The application method of residues of formaldehyde assay kit, includes the following steps in a kind of above-mentioned mushroom：It will be in test agent
Citric acid, AHMT solution, glyceryl triacetate, lithium hydroxide, sodium ethylene diamine tetracetate and color developing agent be blended in detection pipe,
Then white cloth is immersed into mixed liquid dipping, mushroom sample to be measured is then put into detection pipe, after 10min, take out cloth
Item dries cloth, and the color of the color of cloth and colorimetric card is compared to the content of formaldehyde in i.e. detectable mushroom.
Performance test
The kit box being prepared using the embodiment of the present invention common commercially available kit (as a comparison case) in the market
It is detected, the data obtained is included in table 1.
The different kit detection formaldehyde comparisons of table 1
| Group | Developing time (min) | Accuracy rate (%) |
| Embodiment 1 | 5 | 98.6 |
| Embodiment 2 | 5 | 98.2 |
| Embodiment 3 | 6 | 97.8 |
| Comparative example | 10 | 87.4 |
As shown in table 1, using the content of formaldehyde in the kit detection mushroom of the present invention, developing time is fast, and accuracy rate
It is high.
Specific embodiments of the present invention are described above, but the present invention is not limited to this.Based in the present invention
Embodiment, those of ordinary skill in the art made various modifications or repair under the premise of not making any creative work
Change, do not affect the essence of the present invention, belongs to the range that the present invention is protected.The scope of the present invention is intended to be wanted by right
Book and its equivalent form is asked to be limited.
Claims (7)
1. residues of formaldehyde assay kit in a kind of mushroom, by supporter, color developing agent, white cloth and colorimetric card containing test agent
Composition, which is characterized in that the test agent mainly contains following raw material：Citric acid, AHMT solution, glyceryl triacetate, hydrogen-oxygen
Change lithium and sodium ethylene diamine tetracetate.
2. residues of formaldehyde assay kit in mushroom according to claim 1, which is characterized in that the test agent is by weight
Number meter mainly contains following raw material：15-23 parts of citric acid, 9-16 parts of AHMT solution, 4-11 parts of glyceryl triacetate, hydrogen-oxygen
Change 3-9 parts of 6-17 parts of lithium and sodium ethylene diamine tetracetate.
3. residues of formaldehyde assay kit in mushroom according to claim 1, which is characterized in that the test agent is by weight
Number meter mainly contains following raw material：19 parts of citric acid, 13 parts of AHMT solution, 7 parts of glyceryl triacetate, 11 parts of lithium hydroxide
With 4 parts of sodium ethylene diamine tetracetate.
4. residues of formaldehyde assay kit in mushroom according to claim 1, which is characterized in that the color developing agent is quality
A concentration of 1% ammonium ferric sulfate solution.
5. residues of formaldehyde assay kit in mushroom according to claim 1, which is characterized in that above support is with holes
Plastic plate, test agent is placed in volume weight tube, and the volume weight tube is positioned in the support plate.
6. residues of formaldehyde assay kit in mushroom according to claim 1, which is characterized in that the white cloth is nothing
Woven fabric.
7. the application method of residues of formaldehyde assay kit in a kind of mushroom as described in claim 1, which is characterized in that including
Following steps：By citric acid, AHMT solution, glyceryl triacetate, lithium hydroxide, the sodium ethylene diamine tetracetate and aobvious in test agent
Toner is blended in detection pipe, and white cloth is then immersed mixed liquid dipping, mushroom sample to be measured is then put into detection pipe
Product take out cloth, dry cloth after 5-10min, and the color of the color of cloth and colorimetric card is compared i.e. detectable perfume
The content of formaldehyde in mushroom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810349153.5A CN108663359A (en) | 2018-04-18 | 2018-04-18 | Residues of formaldehyde assay kit in a kind of mushroom |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810349153.5A CN108663359A (en) | 2018-04-18 | 2018-04-18 | Residues of formaldehyde assay kit in a kind of mushroom |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108663359A true CN108663359A (en) | 2018-10-16 |
Family
ID=63780118
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810349153.5A Withdrawn CN108663359A (en) | 2018-04-18 | 2018-04-18 | Residues of formaldehyde assay kit in a kind of mushroom |
Country Status (1)
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107340289A (en) * | 2017-06-28 | 2017-11-10 | 防城港市质量技术监督局 | A kind of formaldehyde determination kit |
| CN107389670A (en) * | 2017-06-28 | 2017-11-24 | 防城港市质量技术监督局 | A kind of kit for determining formaldehyde in air |
-
2018
- 2018-04-18 CN CN201810349153.5A patent/CN108663359A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107340289A (en) * | 2017-06-28 | 2017-11-10 | 防城港市质量技术监督局 | A kind of formaldehyde determination kit |
| CN107389670A (en) * | 2017-06-28 | 2017-11-24 | 防城港市质量技术监督局 | A kind of kit for determining formaldehyde in air |
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Application publication date: 20181016 |
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