CN108653358A - A kind of preparation method of root of Low serpentroot chromocor extract - Google Patents
A kind of preparation method of root of Low serpentroot chromocor extract Download PDFInfo
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- CN108653358A CN108653358A CN201810770949.8A CN201810770949A CN108653358A CN 108653358 A CN108653358 A CN 108653358A CN 201810770949 A CN201810770949 A CN 201810770949A CN 108653358 A CN108653358 A CN 108653358A
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- preparation
- serpentroot
- root
- low
- microwave
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
The invention discloses a kind of preparation methods of root of Low serpentroot chromocor extract, and steps are as follows：Root of Low serpentroot medicinal powder 70 80% ethyl alcohol of volume fraction is extracted 24 times in Microwave Extraction Apparatus, 40 50min, microwave temperature are 55 65 DEG C every time, and microwave power is 840 860W, merges extracting solution, and extracting solution is concentrated under reduced pressure to give medicinal extract；By medicinal extract to use large pore resin absorption column chromatographic isolation after appropriate water dissolution, pure water, ethanol gradient elution are used successively, collects 70% alcohol elution, then detach through silica gel column chromatography, use volume ratio 65 successively:1、45:1、25:1、16:1、5:1 chloroform methanol elution, collects 16:1 elution fraction, concentrates to obtain the final product.The method that the present invention uses microwave radiation exaraction, can fast and effectively extract flavones ingredient in root of Low serpentroot, have neuroprotection, can be used for drug preparation.
Description
Technical field
The present invention relates to a kind of preparation methods of root of Low serpentroot chromocor extract.
Background technology
Cerebral arterial thrombosis（Ischemicstroke, IS）With very high case fatality rate and disability rate, the mankind are seriously affected
Life and health and quality of life.Ischemical reperfusion injury is most important link in cerebral arterial thrombosis pathogenic process, is occurred
Mechanism may include excessive generations of free radical, intracellular calcium overload, toxicity of excitatory amino acid act on, leucocyte high aggregation and
The shortage etc. of high energy phosphate compound.In recent years, multinomial research confirms plant flavone substance to after cerebral ischemia re-pouring injured
Tissue and nerve have certain protective effect.Root of Low serpentroot（Scorzonera sinensisLipsch）Nature and flavor are pungent, cool, enter
Lung, liver two pass through, and effect is clearing heat and detoxicating, detoxification sore treatment, are that the important medicine of northern China eats wild feverfew, resourceful.
Invention content
The purpose of the present invention is to provide a kind of preparation methods of root of Low serpentroot chromocor extract.
The present invention is realized by following technical solution：
A kind of preparation method of root of Low serpentroot chromocor extract, includes the following steps：By root of Low serpentroot medicinal powder volume point
Number 70-80% ethyl alcohol extracts 2-4 times in Microwave Extraction Apparatus, and each 40-50min, microwave temperature is 55-65 DEG C, and microwave power is
840-860W, merges extracting solution, and extracting solution is concentrated under reduced pressure to give medicinal extract；By medicinal extract to use macroporous absorbent resin after appropriate water dissolution
Pillar layer separation is collected 70% ethyl alcohol and is washed successively with pure water, the ethanol gradient elution of volume fraction 10%, 35%, 60%, 70%, 95%
De- position, then detached through silica gel column chromatography, volume ratio 65 is used successively:1、45:1、25:1、16:1、5:1 chloroform-methanol elution,
Collect 16:1 elution fraction, concentrates to obtain the final product.
Preferably, in the preparation method, by root of Low serpentroot medicinal powder with 75% ethyl alcohol of volume fraction in microwave abstracting
It is extracted in instrument.
Preferably, it in the preparation method, extracts 3 times.
Preferably, in the preparation method, 45min is extracted every time.
Preferably, in the preparation method, microwave temperature is 60 DEG C.
Preferably, in the preparation method, microwave power 850W.
Preferably, in the preparation method, 1kg root of Low serpentroot is extracted using 5-10L solvents.
Preferably, in the preparation method, by medicinal extract first to use macroporous absorption again with cotton filtering after appropriate water dissolution
Resin column chromatography detaches.
Preferably, in the preparation method, the 4-6mL water dissolutions of 1g medicinal extract.
Preferably, in the preparation method, the large pore resin absorption column is AB-8 types.
The technology of the present invention effect：
The method that the present invention uses microwave radiation exaraction, can fast and effectively extract flavones ingredient in root of Low serpentroot, to brain
The expression of ischemical reperfusion injury rat hippocampus area A β and its precursor APP has an inhibiting effect, and has neuroprotection, can be with
Active component as root of Low serpentroot is prepared applied to drug.
Specific implementation mode
The essentiality content of the present invention is specifically introduced with reference to embodiment.
Embodiment 1
A kind of preparation method of root of Low serpentroot chromocor extract, includes the following steps：By root of Low serpentroot medicinal powder volume point
Several 75% ethyl alcohol extract 3 times in Microwave Extraction Apparatus, each 45min, and microwave temperature is 60 DEG C, and microwave power 850W, merging carries
Liquid, extracting solution is taken to be concentrated under reduced pressure to give medicinal extract；By medicinal extract first to use AB-8 type macropores to inhale again with cotton filtering after appropriate water dissolution
Attached resin column chromatography separation collects 70% successively with pure water, the ethanol gradient elution of volume fraction 10%, 35%, 60%, 70%, 95%
Alcohol elution, then detached through silica gel column chromatography, volume ratio 65 is used successively:1、45:1、25:1、16:1、5:1 chloroform-first
Alcohol elutes, and collects 16:1 elution fraction, concentrates to obtain the final product.
Wherein, 1kg root of Low serpentroot is extracted using 7L solvents, the 5mL water dissolutions of 1g medicinal extract.
Embodiment 2
A kind of preparation method of root of Low serpentroot chromocor extract, includes the following steps：By root of Low serpentroot medicinal powder volume point
Several 70% ethyl alcohol extract 2 times in Microwave Extraction Apparatus, each 40min, and microwave temperature is 55 DEG C, and microwave power 840W, merging carries
Liquid, extracting solution is taken to be concentrated under reduced pressure to give medicinal extract；By medicinal extract first to use AB-8 type macropores to inhale again with cotton filtering after appropriate water dissolution
Attached resin column chromatography separation collects 70% successively with pure water, the ethanol gradient elution of volume fraction 10%, 35%, 60%, 70%, 95%
Alcohol elution, then detached through silica gel column chromatography, volume ratio 65 is used successively:1、45:1、25:1、16:1、5:1 chloroform-first
Alcohol elutes, and collects 16:1 elution fraction, concentrates to obtain the final product.
Wherein, 1kg root of Low serpentroot is extracted using 5L solvents, the 4mL water dissolutions of 1g medicinal extract.
Embodiment 3
A kind of preparation method of root of Low serpentroot chromocor extract, includes the following steps：By root of Low serpentroot medicinal powder volume point
Several 80% ethyl alcohol extract 4 times in Microwave Extraction Apparatus, each 50min, and microwave temperature is 65 DEG C, and microwave power 860W, merging carries
Liquid, extracting solution is taken to be concentrated under reduced pressure to give medicinal extract；By medicinal extract first to use AB-8 type macropores to inhale again with cotton filtering after appropriate water dissolution
Attached resin column chromatography separation collects 70% successively with pure water, the ethanol gradient elution of volume fraction 10%, 35%, 60%, 70%, 95%
Alcohol elution, then detached through silica gel column chromatography, volume ratio 65 is used successively:1、45:1、25:1、16:1、5:1 chloroform-first
Alcohol elutes, and collects 16:1 elution fraction, concentrates to obtain the final product.
Wherein, 1kg root of Low serpentroot is extracted using 10L solvents, the 6mL water dissolutions of 1g medicinal extract.
The method that the present invention uses microwave radiation exaraction, can fast and effectively extract flavones ingredient in root of Low serpentroot,
There is inhibiting effect to the expression of cerebral ischemia-reperfusion injury in rats hippocampus A β and its precursor APP, and there is neuroprotection,
It can be prepared applied to drug as the active component of root of Low serpentroot.
Claims (10)
1. a kind of preparation method of root of Low serpentroot chromocor extract, it is characterised in that include the following steps：By root of Low serpentroot medicinal material
Powder is extracted 2-4 times with volume fraction 70-80% ethyl alcohol in Microwave Extraction Apparatus, each 40-50min, microwave temperature 55-65
DEG C, microwave power 840-860W merges extracting solution, and extracting solution is concentrated under reduced pressure to give medicinal extract；By medicinal extract with appropriate water dissolution after
With large pore resin absorption column chromatographic isolation, washed successively with the ethanol gradient of pure water, volume fraction 10%, 35%, 60%, 70%, 95%
It is de-, 70% alcohol elution is collected, then detach through silica gel column chromatography, uses volume ratio 65 successively:1、45:1、25:1、16:1、5:1
Chloroform-methanol elution, collect 16:1 elution fraction, concentrates to obtain the final product.
2. preparation method according to claim 1, it is characterised in that：By root of Low serpentroot medicinal powder volume fraction 75%
Ethyl alcohol extracts in Microwave Extraction Apparatus.
3. preparation method according to claim 2, it is characterised in that：Extraction 3 times.
4. preparation method according to claim 3, it is characterised in that：Extraction 45min every time.
5. preparation method according to claim 1, it is characterised in that：Microwave temperature is 60 DEG C.
6. preparation method according to claim 1, it is characterised in that：Microwave power is 850W.
7. preparation method according to claim 1, it is characterised in that：1kg root of Low serpentroot is carried using 5-10L solvents
It takes.
8. preparation method according to claim 1, it is characterised in that：By medicinal extract first to be filtered with cotton after appropriate water dissolution
Large pore resin absorption column chromatographic isolation is used again.
9. preparation method according to claim 8, it is characterised in that：The 4-6mL water dissolutions of 1g medicinal extract.
10. preparation method according to claim 8, it is characterised in that：The large pore resin absorption column is AB-8 types.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810770949.8A CN108653358A (en) | 2018-07-13 | 2018-07-13 | A kind of preparation method of root of Low serpentroot chromocor extract |
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| CN201810770949.8A CN108653358A (en) | 2018-07-13 | 2018-07-13 | A kind of preparation method of root of Low serpentroot chromocor extract |
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| CN108653358A true CN108653358A (en) | 2018-10-16 |
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| CN201810770949.8A Withdrawn CN108653358A (en) | 2018-07-13 | 2018-07-13 | A kind of preparation method of root of Low serpentroot chromocor extract |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109897030A (en) * | 2019-03-26 | 2019-06-18 | 吉林大学 | A kind of 6 "-oxygen-coffee acyl orientin preparation methods |
-
2018
- 2018-07-13 CN CN201810770949.8A patent/CN108653358A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109897030A (en) * | 2019-03-26 | 2019-06-18 | 吉林大学 | A kind of 6 "-oxygen-coffee acyl orientin preparation methods |
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Application publication date: 20181016 |