CN108642049A - A kind of cutting type reagent extraction amplification device - Google Patents
A kind of cutting type reagent extraction amplification device Download PDFInfo
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- CN108642049A CN108642049A CN201810605074.6A CN201810605074A CN108642049A CN 108642049 A CN108642049 A CN 108642049A CN 201810605074 A CN201810605074 A CN 201810605074A CN 108642049 A CN108642049 A CN 108642049A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
Abstract
The present invention provides a kind of cutting type reagents to extract amplification device comprising：Extract storehouse comprising cracking mechanism, washing mechanism and the elution mechanism being sequentially distributed according to sample flow direction；It cracks and is equipped with lytic reagent and magnetisable material in mechanism；Washing mechanism includes the first oil groove and sink, and cleaning solution is equipped in sink；First oil groove is located between cracking mechanism and sink；It includes the second oil groove and elution slot to elute mechanism, and eluent is equipped in elution slot；Second oil groove is located between sink and elution slot；It is equipped with oil phase in first oil groove and the second oil groove；Mobilizable partition is equipped between adjacent two slot；It cracks and is equipped with mobilizable partition between mechanism and the first oil groove；Driving mechanism comprising the sliding block of sliding can be flowed to according to sample；Sliding block is equipped with magnet；Amplification pipe is located at elution mechanism downstream；Amplification pipe is connected to elution slot.The present invention can by the extraction of nucleic acid and augmentation detection under totally-enclosed environment integration, high-efficient simple, as a result accurately.
Description
Technical field
The present invention relates to a kind of cutting type reagents to extract amplification device.
Background technology
Nucleic acid diagnostic assay method based on round pcr is the core technology in molecule diagnosis.Nucleic acid is carried automatically
It takes process and PCR amplification detection process mutually integrated, integrated nucleic acid extraction and augmentation detection system is built, for further
Nucleic acid diagnostic assay efficiency is improved, realizes that the automation of entire nucleic acid diagnostic assay process has important practical significance.
Chinese patent 201120449250.5 discloses a kind of nucleic acid amplification and detection reaction tube, including mutual cooperation tube body
With pipe lid, tube body includes the liquid storage area being located above and underlying nucleic acid amplification area, completes to react in nucleic acid amplification area
And the real-time detection means such as fluorescence signal acquisition；Pipe lid is internally provided with medium altitude groove, and the volume in nucleic acid amplification area is respectively smaller than
Or equal to the volume of liquid storage area and the volume of medium altitude groove.
Chinese patent 201521043284.9 discloses a kind of isothermal nucleic acid amplification device and experimental method, including tablet is former
Position nucleic acid amplification chip, the chip is by being bonded to each other, slidable upper and lower layer carrier is constituted, and upper and lower layer carrier is same size
Rectangle, when lower layer is fixed, upper layer carrier can relative friction sliding.Various open is opened up on the upper binding face of lower layer's carrier
The downward groove runner of mouth, microcavity, open up groove discharge orifice, the microcavity of various opening upwards on the lower binding face of upper layer carrier,
And some import and export through-holes.
Chinese patent 201620284715.9 discloses a kind of multi-functional nucleic acid augmentative instrument, including control system, switch electricity
Source, host computer, heating circulation system and sample module, control system connecting valve power supply, host computer and heating circulation system, add
The top of heat circulating system connects sample module, 384 orifice plates of the sample module equipped with 0.2mL, 96 orifice plates, 48 orifice plates and
12 orifice plates of 1.5mL.
Chinese patent application 201710163203.6 discloses integrated nucleic acid extraction and augmentation detection system, the system packet
Include disposable micro-fluidic chip and mating detection device.Wherein micro-fluidic chip includes reaction member and waste liquid storage unit, core
When piece works, by being mutually mixed between vibration module realization reaction reagent, between reaction reagent and magnetic bead, driven by magnetic bead
Dynamic model block, be sequentially completed nucleic acid absorption, purifying and etc.；PCR amplification reagent is allowed to rinse magnetic bead, elution by fluid drive module
Amplification template on magnetic bead passes through heating module and fluorescent collecting mould into the convection current PCR reactors built by vertical channel
Block realizes pcr amplification reaction and detection.
The defect of above-mentioned technical proposal is：
1, complicated, need vacuum pump；And lysate is more sticky, vacuum pump operation easy tos produce bubble, influences to carry
It takes；
2, waste liquid can pass through nucleic acid amplification test chamber, there is waste liquid residual contamination, influence nucleic acid amplification detection；
Both 3, detection process is not closed, and the environment outside detection device is caused to be contacted with the reagent in detection device, cause
There are the risks of cross contamination；
4, detection process is cumbersome, and multiple links need to increase manual operations；
5, detection time is long.
Therefore, how to provide it is a kind of there is no vacuum pump, instrument and equipment structure is simpler；Bubble is not generated, is conducive to extract
It is smoothed out, obtains the higher nucleic acid of purity；With individual nucleic acid amplification test chamber, there is no waste liquid remaining influence；It detected
Journey closing, the reagent that can effectively avoid external environment and detection reagent cross contamination, which extract amplification device and become industry, to be needed to solve
Certainly the problem of.
Invention content
In view of the shortcomings of the prior art, the object of the present invention is to provide a kind of cutting type reagents to extract amplification device, knot
Structure is simple, efficiently and accurately, and detection process closing can effectively avoid external environment and detection reagent cross contamination.
To achieve the goals above, the present invention provides a kind of cutting type reagents to extract amplification device, reagent extraction amplification
Device includes：
Storehouse is extracted, extraction storehouse includes cracking mechanism, washing mechanism and the elution mechanism being sequentially distributed according to sample flow direction；It splits
It solves and is equipped with lytic reagent and magnetisable material in mechanism；Washing mechanism includes the first oil groove and sink, and washing is equipped in sink
Liquid；First oil groove is located between cracking mechanism and sink；It includes the second oil groove and elution slot to elute mechanism, elutes and is equipped in slot
Eluent；Second oil groove is located between sink and elution slot；It is equipped with oil phase in first oil groove and the second oil groove；Adjacent two slot
Between be equipped with mobilizable partition；It cracks and is equipped with mobilizable partition between mechanism and the first oil groove；Partition is that can move up and down
Diaphragm seal or tear-away fixed card；
Driving mechanism, driving mechanism include that the sliding block of sliding can be flowed to according to sample；Sliding block is equipped with magnet；
Amplification pipe, amplification pipe are located at elution mechanism downstream；Amplification pipe is connected to elution slot.
In the present invention, extraction storehouse can be made of elastic material.
In the present invention, extract in storehouse, for the quantity of slot depending on needed for carried reagent or solid matter, capacity can be not to the utmost
It is identical.
In the present invention, driving mechanism further comprises that the power part for driving sliding block to run, power part can be electricity
Machine or cylinder.
In the present invention, the nucleic acid generated after sample cracking is attached on magnetisable material；When slide block movement, driven by magnet
Magnetisable material moves, to drive nucleic acid movement.
In the present invention, each slot is along lineal layout；In use, extraction storehouse can be horizontally arranged, vertically place or
Slant setting.
In the present invention, when sliding block is by separating, driving mechanism can make partition fail, and the slot for separating both sides communicates；Part
Separate permanent failure, partial partition restores once again later in sliding block warp.
The present invention solve nucleic acid extraction and nucleic acid amplification detection cannot integration, nucleic acid extraction and nucleic acid amplification detected
The technical issues of journey trivial operations, high-efficient simple, as a result accurately.
According to another specific implementation mode of the present invention, cracking mechanism include the magnetic slot being sequentially distributed according to sample flow direction,
Splitter and engagement groove；It cracks in mechanism, mobilizable partition is equipped between adjacent two slot；It is set between engagement groove and the first oil groove
There is mobilizable partition.
Sample lid further comprises the sample for being located at magnetic slot upstream according to another specific implementation mode of the present invention, cracking mechanism
This slot, sample are located in sample slot；Lytic reagent includes that cracking reinforcing agent and cracking combine liquid, is respectively arranged on splitter and combination
In slot；Magnetisable material is set in magnetic slot.
In the present solution, sample slot is equipped with the feed inlet for adding sample, feed inlet is equipped with sample lid, is used for sample
Add rear enclosed sample slot.
According to another specific implementation mode of the present invention, extraction storehouse further comprises that buffering liquid groove, buffering liquid groove are located at elution
Slot downstream, and it is equipped with mobilizable partition between elution slot；Buffering liquid groove is located at amplification pipe upstream, and is communicated with amplification pipe.
In the present solution, buffering liquid groove is connected with amplification pipe by connector, connector is equipped with discharge port；Sample is by going out
Material mouth flows into amplification pipe from buffering liquid groove.
In addition it is also possible to not set amplification pipe, and carried out amplification reaction in buffering liquid groove；At this point, it is not necessary that connector is arranged,
Discharge port is directly set on buffering liquid groove, the outflow for sample after amplified reaction.
According to another specific implementation mode of the present invention, sealing moving up and down is equipped between the first oil groove and sink
Piece；It is equipped with diaphragm seal moving up and down between second oil groove and elution slot.
It according to another specific implementation mode of the present invention, cracks in mechanism, tear-away fixing card is equipped between adjacent two slot
Piece；Tear-away fixed card is equipped between engagement groove and the first oil groove；It is equipped between sink and the second oil groove tear-away
Fixed card；It elutes and is equipped with tear-away fixed card between slot and buffering liquid groove.
According to another specific implementation mode of the present invention, extraction storehouse further comprises that elastic sealing film, elastic sealing film are located at
Diaphragm seal and fixed card lower section, and compressed by diaphragm seal and fixed card.
According to another specific implementation mode of the present invention, sliding block is equipped with for jacking up diaphragm seal or fixed card being made to fall off
Wedge block.
According to another specific implementation mode of the present invention, magnet is located at center, is permanent magnet or electromagnet.
According to another specific implementation mode of the present invention, magnetisable material is magnetic bead.
In the present solution, reagent extraction amplification device further comprises upper cover, holder and bottom cover；Holder is fixed on extraction storehouse
On, the two is combined by modes such as bonding, cementing, hot adhesions；Upper cover and bottom cover are detachably connected, and the two surrounds a sky
Chamber, extracts storehouse and holder is located in cavity；Holder is equipped with upper cover mounting hole, for being connect with upper cover.
It extracts orlop portion to seal using sealer, sealer can be sealed loaded on the liquid or solid in extraction storehouse in each slot；Envelope
Film can be combined with extraction storehouse by the modes such as Nian Jie, cementing, hot adhesion；Elastic sealing film is located above sealer, in fixation
It under the pressuring action of card and diaphragm seal, is bonded with sealer, the liquid or solid between each slot forms isolation.
Fixed card is blocked for reagent, prevents the reagent in adjacent two slot from mutually mixing, and quantity is depending on reaction requires；
Fixed card both sides connect upper cover bottom by weak support respectively；Weak support is easy to for being connected and fixed card and upper cover
It is cut off.
Holder is equipped with the limiting slot for limiting diaphragm seal degree of freedom, and limiting slot is correspondingly arranged with diaphragm seal；Diaphragm seal
For preventing the reagent in adjacent two slot from mutually mixing；Upper cover inside top is equipped with spring mounting hole, is correspondingly arranged with diaphragm seal；It is close
Mounting includes the protrusion for compressing elastic sealing film and the axis pin for coordinating with spring, and protrusion is located at diaphragm seal bottom, pin
Axle position is at the top of diaphragm seal；Spring side is installed in spring mounting hole, and the other side is sleeved on axis pin.
The number of wedge block is two, is located at magnet both sides；Sliding block is located on the outside of bottom cover.
The driver slot for magnet movement is equipped in the middle part of bottom cover, both sides are equipped with the slideway for wedge block movement；Sliding block is transported
When dynamic, extraction position in storehouse is between two wedge blocks；Weak support can be cut off in wedge block, and fixed card is made to fall off；Or pass through its top
The inclined-plane in portion jacks up diaphragm seal.
Upper lid is equipped with extrusion cavities, and extrusion cavities are located above buffering liquid groove；Openable and closable pressing plate, pressing plate are connected on extrusion cavities
It can extrude elution slot and buffering liquid groove when moving down.
According to another specific implementation mode of the present invention, the amplification examination expanded for realizing nucleic acid increment is equipped in amplification pipe
Agent, amplifing reagent are PCR platforms reagent or constant-temperature amplification reagent.
The operating principle of the present invention is following (magnet is electromagnet)：
1, sample slot is added in sample, closes feed inlet；Meanwhile sliding block is placed in below sample slot；At this point, magnet is not yet logical
Electricity does not have magnetism；
2, sliding block is moved to amplification pipe direction, and partition failure, liquid flows into magnetic slot；
3, sliding block continues to move to, and partition failure, liquid flows into splitter；
4, sliding block continues to move to, and partition failure, liquid flows into engagement groove；
5, magnetisable material is uniformly diffused into cracking and combines in liquid；Sample is cleaved, and discharges nucleic acid；Nucleic acid is with magnetisable material knot
It is combined；
6, magnet is powered, and starts have magnetism；Sliding block continues to move to, and partition failure, magnetisable material flows into the first oil groove；
7, sliding block continues to move to, and separates temporarily failure, and magnetisable material flows into sink；When sliding block is located at below sink
When, the partition between the first oil groove and sink restores, and continues to separate the first oil groove and sink；
8, magnet powers off, and magnetisable material is uniformly diffused into cleaning solution；Impurity on magnetisable material is washed off, and is transferred to
In cleaning solution；
9, magnet is powered, and sliding block continues to move to, and partition failure, magnetisable material flows into the second oil groove；
10, sliding block continues to move to, and separates temporarily failure, and magnetisable material flows into elution slot；When sliding block is located at below elution slot
When, the partition between the second oil groove and elution slot restores, and continues to separate the second oil groove and elution slot；
11, magnet powers off, and magnetisable material is uniformly diffused into eluent；Nucleic acid on magnetisable material is washed off, and is shifted
Into eluent；
12, magnet is powered, and sliding block continues to move to, and partition failure, liquid flows into buffering liquid groove；Sliding block back moves, and is parked in
It elutes below slot, magnetisable material is made to be retained in elution slot；Nucleic acid stays in buffering liquid groove；
13, in the case where the external world squeezes, the liquid in buffering liquid groove flows into amplification pipe, and carries out nucleic acid amplification in amplification is managed.
Compared with prior art, the present invention has following advantageous effect：
Nucleic acid extraction and nucleic acid amplification can be detected seamless union by the present invention, it is only necessary to sample be added, can be obtained in 1 hour
As a result；Whole process carries out under totally-enclosed environment without being further added by operating procedure, can effectively avoid external environment and inspection
Test agent cross contamination；Lysate, cleaning solution, eluent are separately fixed at individual liquid storage cavity, and with oil phase by liquid
It separates two-by-two, will not be mutually in direct contact between liquid, avoid cross contamination；Amplification pipe be also be separately provided, be more advantageous into
Row amplified reaction.
The present invention is described in further detail below in conjunction with the accompanying drawings.
Description of the drawings
Fig. 1 is the explosive view of the reagent extraction amplification device of embodiment 1；
Fig. 2 is the sectional view of the reagent extraction amplification device of embodiment 1；
Fig. 3 is the structural schematic diagram of the holder of embodiment 1；
Fig. 4 is the structural schematic diagram of the upper cover of embodiment 1；
Fig. 5 is the structural schematic diagram of the sliding block of embodiment 1；
Fig. 6 is the structural schematic diagram of the diaphragm seal of embodiment 1；
Fig. 7 is the overall structure diagram of the reagent extraction amplification device of embodiment 1；
Fig. 8 is the structural schematic diagram of the bottom cover of embodiment 1.
Specific implementation mode
Embodiment 1
A kind of cutting type reagent extraction amplification device is present embodiments provided, as shown in figures 1-8 comprising extraction storehouse 1,
Crack mechanism 2, washing mechanism 3, elution mechanism 4, driving mechanism 5, amplification pipe 6, upper cover 7, holder 8 and bottom cover 9.
Wherein, extraction storehouse 1 is made of elastic material comprising flows to the cracking mechanism 2 being sequentially distributed, washing according to sample
Mechanism 3, elution mechanism 4, buffering liquid groove 101 and elastic sealing film 104；Holder 8 is fixed on extraction storehouse 1, and the two is by cohering
Agent bonds；Upper cover 7 and bottom cover 9 are detachably connected, and the two surrounds a cavity, extract storehouse 1 and holder 8 is located in cavity；Branch
Frame 8 is equipped with upper cover mounting hole 801, for being connect with upper cover 7.
It cracks and is equipped with lytic reagent and magnetisable material in mechanism 2；Magnetisable material is magnetic bead.
Washing mechanism 3 includes the first oil groove 301 and sink 302, and cleaning solution is equipped in sink 302；First oil groove 301
Between cracking mechanism 2 and sink 302.
It includes the second oil groove 401 and elution slot 402 to elute mechanism 4, and eluent is equipped in elution slot 402；Second oil groove 401
Between sink 302 and elution slot 402；It is equipped with oil phase in first oil groove 301 and the second oil groove 401；Adjacent two slot it
Between be equipped with mobilizable partition；It cracks and is equipped with mobilizable partition between mechanism 2 and the first oil groove 301.
Cracking mechanism 2 includes sample slot 201, magnetic slot 202, splitter 203 and the knot being sequentially distributed according to sample flow direction
Close slot 204；It cracks in mechanism 2, mobilizable partition is equipped between adjacent two slot；It is set between engagement groove 204 and the first oil groove 301
There is mobilizable partition；Sample slot 201 is equipped with the feed inlet 205 for adding sample, and feed inlet 205 is equipped with sample lid
206, for sample addition rear enclosed sample slot 201.
Lytic reagent includes that cracking reinforcing agent and cracking combine liquid, is respectively arranged in splitter 203 and engagement groove 204；Magnetic
Property substance be set in magnetic slot 202.
Driving mechanism 5 includes the sliding block 501 that sliding can be flowed to according to sample and the power part for driving sliding block 501 to run
Part (not shown), power part are motor；Sliding block 501 is equipped with magnet 502；Magnet 502 is located at 501 center of sliding block, is
Electromagnet.
It expands pipe 6 and is located at 4 downstream of elution mechanism；Amplification pipe 6 is connected to elution slot 402；Amplification pipe in be equipped with for realizing
The amplifing reagent of nucleic acid increment amplification, amplifing reagent are PCR platform reagents.
Buffering liquid groove 101 is located at 402 downstream of elution slot, and is equipped with mobilizable partition between elution slot 402；Buffer solution
Slot 101 is located at 6 upstream of amplification pipe, and is communicated with amplification pipe 6；Buffering liquid groove 101 is connected with amplification pipe 6 by connector 102, even
Connector 102 is equipped with discharge port 103；Sample flows into amplification pipe 6 by discharge port 103 from buffering liquid groove 101；Upper cover 7 is equipped with
Extrusion cavities 701, extrusion cavities 701 are located at 101 top of buffering liquid groove；Openable and closable pressing plate 702, pressing plate 702 are connected on extrusion cavities 701
It can extrude elution slot 402 and buffering liquid groove 101 when moving down.
Diaphragm seal 11 moving up and down is equipped between first oil groove 301 and sink 302；Second oil groove 401 and elution
Diaphragm seal 11 moving up and down is equipped between slot 402；Holder 8 is equipped with the limiting slot for limiting 11 degree of freedom of diaphragm seal
802, limiting slot 802 is correspondingly arranged with diaphragm seal 11；Diaphragm seal 11 is for preventing the reagent in adjacent two slot from mutually mixing；In upper cover 7
Side roof part is equipped with spring mounting hole 703, is correspondingly arranged with diaphragm seal 11；Diaphragm seal 11 includes for compressing elastic sealing film
104 protrusion 1101 and axis pin 1102 for coordinating with spring 13, protrusion 1101 are located at 11 bottom of diaphragm seal, axis pin 1102
In 11 top of diaphragm seal；13 side of spring is installed in spring mounting hole 703, and the other side is sleeved on axis pin 1102.
It cracks in mechanism 2, tear-away fixed card 12 is equipped between adjacent two slot；Engagement groove 204 and the first oil groove 301
Between be equipped with tear-away fixed card 12；Tear-away fixed card 12 is equipped between sink 302 and the second oil groove 401；
It elutes and is equipped with tear-away fixed card 12 between slot 402 and buffering liquid groove 101；Fixed card 12 is blocked for reagent, is prevented
Reagent in adjacent two slot mutually mixes；12 both sides of fixed card pass through 1,201 7 bottom of connection upper cover of weak support respectively.
Elastic sealing film 104 is located at diaphragm seal 11 and 12 lower section of fixed card, and is pressed by diaphragm seal 11 and fixed card 12
Tightly.
Sliding block 501 is equipped with the wedge block 503 for jacking up diaphragm seal 11 or fixed card 12 being made to fall off；Wedge block 503
Number be two, be located at 502 both sides of magnet；Sliding block 501 is located at 9 outside of bottom cover；9 middle part of bottom cover, which is equipped with, is used for magnet
The driver slot 901 of 502 movements, both sides are equipped with the slideway 902 moved for wedge block 503；When sliding block 501 moves, 1, storehouse is extracted
Between two wedge blocks 503；Weak support 1201 can be cut off in wedge block 503, and fixed card 12 is made to fall off；Or pass through its top
Inclined-plane jack up diaphragm seal 11.
In the present embodiment, extraction 1 bottom of storehouse is sealed using sealer 105, and sealer 105 can be sealed loaded on each in extraction storehouse 1
Liquid or solid in slot；Sealer 105 is bonded with extraction storehouse 1 by adhesive；Elastic sealing film 104 is located at 105 top of sealer,
It is bonded under the pressuring action of fixed card 12 and diaphragm seal 11 with sealer 105, and the liquid or solid between each slot is formed
Isolation.
The application method of the present embodiment is as follows：
1, sample lid 206 is removed, sample is added to sample slot 201, covers sample lid 206；
2, under power part driving, sliding block 501 is moved toward 6 direction of amplification pipe；When sliding block 501 passes through fixed card 12
When, wedge block 503 cuts off the weak support 1201 of 12 both sides of fixed card；Fixed card 12 falls off, sealing function failure；It is fixed
The elastic sealing film 104 of 12 top tight sealing of card bounces under natural resiliency effect, and never restores sealing state；It is formed at this time
Access, the fluid connection being located in two side channel of access；
3, sample flows into magnetic slot 202, and magnetisable material carries sample information；Under the effect of magnet 502, sample information is carried
Magnetisable material continued to move to sliding block 501；When by fixed card 12, step 2 is repeated；
4, when sliding block 501 is by diaphragm seal 11, diaphragm seal 11 is bounced when wedge block 503 passes through, and is pushed up by diaphragm seal 11
The elastic sealing film 104 of tightening seal bounces under natural resiliency effect；Magnetisable material passes through with sliding block 501；It is logical in wedge block 503
Later, diaphragm seal 11 compresses elastic sealing film 104 again under the effect of spring 13, blocks its both sides liquid again；
5, when magnetisable material is retained in elution slot 402, and when nucleic acid is stayed in buffering liquid groove 101, pressing plate 702 is to moving down
It is dynamic, squeeze elution slot 402 and buffering liquid groove 101；Liquid in buffering liquid groove 101 flows into amplification pipe 6, and in amplification pipe 6 into
Row PCR amplification.
Embodiment 2
The present embodiment and embodiment 1 difference lies in：Amplification pipe is not set, and is carried out amplification reaction in buffering liquid groove；This
When, it is not necessary that connector is arranged, discharge port is directly set on buffering liquid groove, the outflow for sample after amplified reaction.
Embodiment 3
The present embodiment and embodiment 1 difference lies in：It is equipped with third oil groove, third oil groove between buffering liquid groove and amplification pipe
It is interior to be equipped with oil phase；It is equipped with tear-away fixed card between buffering liquid groove and third oil groove, is set between amplification pipe and third oil groove
There is tear-away fixed card.
Although the present invention is disclosed above with preferred embodiment, it is not limited to the range that the present invention is implemented.Any
The those of ordinary skill in field improves in the invention scope for not departing from the present invention when can make a little, i.e., every according to this hair
Bright done same improvement, should be the scope of the present invention and is covered.
Claims (11)
1. a kind of cutting type reagent extracts amplification device, wherein the reagent extracts amplification device and includes：
Storehouse is extracted, the extraction storehouse includes cracking mechanism, washing mechanism and the elution mechanism being sequentially distributed according to sample flow direction；Institute
It states in cracking mechanism and is equipped with lytic reagent and magnetisable material；The washing mechanism includes the first oil groove and sink, the washing
Cleaning solution is equipped in slot；First oil groove is between the cracking mechanism and the sink；The elution mechanism includes
Second oil groove and elution slot, the elution slot is interior to be equipped with eluent；Second oil groove is located at the sink and the elution
Between slot；It is equipped with oil phase in first oil groove and second oil groove；Mobilizable partition is equipped between adjacent two slot；Institute
It states and is equipped with mobilizable partition between cracking mechanism and first oil groove；The partition is diaphragm seal moving up and down or can
The fixed card to fall off；
Driving mechanism, the driving mechanism include that the sliding block of sliding can be flowed to according to sample；The sliding block is equipped with magnet；
Amplification pipe, the amplification pipe are located at the elution mechanism downstream；The amplification pipe is connected to the elution slot.
2. reagent as described in claim 1 extracts amplification device, wherein the cracking mechanism includes being flowed to successively according to sample
Magnetic slot, splitter and the engagement groove of distribution；In the cracking mechanism, mobilizable partition is equipped between adjacent two slot；It is described
Mobilizable partition is equipped between engagement groove and first oil groove.
3. reagent as claimed in claim 2 extracts amplification device, wherein the cracking mechanism further comprises being located at the magnetic
The sample slot of property slot upstream, sample are located in the sample slot；The lytic reagent includes that cracking reinforcing agent and cracking combine liquid,
It is respectively arranged in the splitter and the engagement groove；The magnetisable material is set in the magnetic slot.
4. reagent as claimed in claim 3 extracts amplification device, wherein the extraction storehouse further comprises buffering liquid groove, institute
It states buffering liquid groove and is located at the elution slot downstream, and be equipped with mobilizable partition between the elution slot；The buffering liquid groove
It is communicated positioned at the amplification pipe upstream, and with the amplification pipe.
5. reagent as claimed in claim 4 extracts amplification device, wherein be equipped between first oil groove and the sink
Diaphragm seal moving up and down；Diaphragm seal moving up and down is equipped between second oil groove and the elution slot.
6. reagent as claimed in claim 5 extracts amplification device, wherein in the cracking mechanism, be equipped between adjacent two slot
Tear-away fixed card；Tear-away fixed card is equipped between the engagement groove and first oil groove；The sink
Tear-away fixed card is equipped between second oil groove；It is equipped between the elution slot and the buffering liquid groove tear-away
Fixed card.
7. reagent as claimed in claim 6 extracts amplification device, wherein the extraction storehouse further comprises elastic sealing film,
The elastic sealing film is located at the diaphragm seal and fixed card lower section, and by the diaphragm seal and the fixed card pressure
Tightly.
8. reagent as claimed in claim 7 extracts amplification device, wherein the sliding block is equipped with for jacking up the diaphragm seal
Or the wedge block for making the fixed card fall off.
9. reagent as described in claim 1 extracts amplification device, wherein the magnet is located at the center, for forever
Magnet or electromagnet.
10. reagent as described in claim 1 extracts amplification device, wherein the magnetisable material is magnetic bead.
11. reagent as described in claim 1 extracts amplification device, wherein be equipped in the amplification pipe and increase for realizing nucleic acid
Measure the amplifing reagent of amplification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810605074.6A CN108642049B (en) | 2018-06-12 | 2018-06-12 | Cut-off type reagent extraction and amplification device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810605074.6A CN108642049B (en) | 2018-06-12 | 2018-06-12 | Cut-off type reagent extraction and amplification device |
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| Publication Number | Publication Date |
|---|---|
| CN108642049A true CN108642049A (en) | 2018-10-12 |
| CN108642049B CN108642049B (en) | 2023-09-29 |
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| CN201810605074.6A Active CN108642049B (en) | 2018-06-12 | 2018-06-12 | Cut-off type reagent extraction and amplification device |
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Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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