CN108634047A - Application and the fungus growing process of a kind of floating derivant and the floating derivant - Google Patents
Application and the fungus growing process of a kind of floating derivant and the floating derivant Download PDFInfo
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- CN108634047A CN108634047A CN201810447590.0A CN201810447590A CN108634047A CN 108634047 A CN108634047 A CN 108634047A CN 201810447590 A CN201810447590 A CN 201810447590A CN 108634047 A CN108634047 A CN 108634047A
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- 238000000034 method Methods 0.000 title claims abstract description 216
- 230000008569 process Effects 0.000 title claims abstract description 198
- 238000007667 floating Methods 0.000 title claims abstract description 196
- 241001597008 Nomeidae Species 0.000 title claims abstract description 90
- 241000233866 Fungi Species 0.000 title claims abstract description 16
- 241000894006 Bacteria Species 0.000 claims abstract description 250
- 239000002775 capsule Substances 0.000 claims abstract description 169
- 239000000725 suspension Substances 0.000 claims abstract description 64
- 239000002994 raw material Substances 0.000 claims abstract description 59
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 45
- 230000001954 sterilising effect Effects 0.000 claims abstract description 38
- 230000001580 bacterial effect Effects 0.000 claims abstract description 34
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 20
- 241000186660 Lactobacillus Species 0.000 claims abstract description 17
- 241001467578 Microbacterium Species 0.000 claims abstract description 17
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 16
- 241000186000 Bifidobacterium Species 0.000 claims abstract description 5
- 244000269722 Thea sinensis Species 0.000 claims description 94
- 235000013616 tea Nutrition 0.000 claims description 80
- 235000006468 Thea sinensis Nutrition 0.000 claims description 19
- 238000002360 preparation method Methods 0.000 claims description 17
- 241001205401 Aspergillus cristatus Species 0.000 claims description 15
- 239000003795 chemical substances by application Substances 0.000 claims description 13
- 235000020334 white tea Nutrition 0.000 claims description 13
- 238000012545 processing Methods 0.000 claims description 12
- 235000020279 black tea Nutrition 0.000 claims description 11
- 235000009569 green tea Nutrition 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 11
- 235000020333 oolong tea Nutrition 0.000 claims description 10
- 239000008223 sterile water Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 235000020338 yellow tea Nutrition 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 7
- 239000000654 additive Substances 0.000 claims description 4
- 230000000996 additive effect Effects 0.000 claims description 4
- 210000004209 hair Anatomy 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 229920000742 Cotton Polymers 0.000 claims description 3
- 239000007900 aqueous suspension Substances 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 239000011814 protection agent Substances 0.000 claims description 3
- 239000004519 grease Substances 0.000 claims description 2
- 239000008267 milk Substances 0.000 claims description 2
- 210000004080 milk Anatomy 0.000 claims description 2
- 235000013336 milk Nutrition 0.000 claims description 2
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 2
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 claims 1
- 241000131482 Bifidobacterium sp. Species 0.000 claims 1
- 241000186610 Lactobacillus sp. Species 0.000 claims 1
- 241000178948 Lactococcus sp. Species 0.000 claims 1
- 241000500375 Microbacterium sp. Species 0.000 claims 1
- 241000589516 Pseudomonas Species 0.000 claims 1
- 230000004913 activation Effects 0.000 claims 1
- 238000001035 drying Methods 0.000 claims 1
- 238000007710 freezing Methods 0.000 claims 1
- 230000008014 freezing Effects 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 42
- 230000000694 effects Effects 0.000 abstract description 16
- 241000194036 Lactococcus Species 0.000 abstract description 5
- 230000000813 microbial effect Effects 0.000 abstract description 5
- 235000013406 prebiotics Nutrition 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 230000002708 enhancing effect Effects 0.000 abstract description 2
- 241001122767 Theaceae Species 0.000 abstract 5
- 238000004519 manufacturing process Methods 0.000 description 13
- 239000000047 product Substances 0.000 description 13
- 241000193749 Bacillus coagulans Species 0.000 description 11
- 229940054340 bacillus coagulans Drugs 0.000 description 11
- 238000005516 engineering process Methods 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 8
- 241000186018 Bifidobacterium adolescentis Species 0.000 description 7
- 210000000481 breast Anatomy 0.000 description 6
- 239000000796 flavoring agent Substances 0.000 description 6
- 235000019634 flavors Nutrition 0.000 description 6
- 239000002253 acid Substances 0.000 description 5
- 238000000855 fermentation Methods 0.000 description 5
- 230000004151 fermentation Effects 0.000 description 5
- 238000004321 preservation Methods 0.000 description 5
- 244000057717 Streptococcus lactis Species 0.000 description 4
- 235000014897 Streptococcus lactis Nutrition 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 230000005855 radiation Effects 0.000 description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 description 3
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 3
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 3
- 239000006041 probiotic Substances 0.000 description 3
- 235000018291 probiotics Nutrition 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 241000228427 Eurotiales Species 0.000 description 2
- 241001136486 Trichocomaceae Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
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- 230000006698 induction Effects 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- 240000006914 Aspalathus linearis Species 0.000 description 1
- 235000012984 Aspalathus linearis Nutrition 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241001136487 Eurotium Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000011449 brick Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013065 commercial product Substances 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000018612 quorum sensing Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical class Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/14—Tea preparations, e.g. using additives
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/137—Delbrueckii
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/231—Lactis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/513—Adolescentes
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Tropical Medicine & Parasitology (AREA)
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Abstract
The present invention relates to a kind of floating derivant and application and the fungus growing process of the floating derivant.The floating derivant includes any one or several combinations in the bacterial strain of the bacterial strain of bacillus, the bacterial strain of lactobacillus, the bacterial strain of Microbacterium, the bacterial strain of lactococcus and Bifidobacterium.It is using the tealeaves fungus growing process step of the floating derivant：Tea raw material sterilization treatment；Add coronoid process dissipate capsule bacterium and floating derivant：The bacteria suspension of the spore suspension of coronoid process dissipate capsule bacterium and floating derivant is added in tea raw material；Tealeaves floating.Floating derivant of the present invention can solve the problems such as Fu tea and other floating type tea product process floating periods are long, floating quality is unstable and is likely to occur harmful miscellaneous bacteria, the present invention can both improve floating efficiency, enhancing floating effect, there is specific microbial safety to ensure again, can provide comprising one or several kinds of prebiotic Fu tea of beneficial microorganism high-quality and other prebiotic tea products of floating type.
Description
Technical field
The invention belongs to microbe applications in food processing technology field, and in particular to a kind of floating derivant and the hair
The application of flower derivant and fungus growing process.
Background technology
Coronoid process dissipate capsule bacterium (Eurotiumcristatum), is commonly called as " golden flower ", is Eurotiale (Eurotiales) Trichocomaceae
(Trichocomaceae) a kind of fungi of capsule category (Eurotium) is dissipated.It improves the immunity of the human body, adjust studies have shown that the bacterium has
It saves human metabolism, lipid-loweringing, decompression, adjust the various health care functions such as blood glucose.Fu tea is a kind of distinctive black tea in China.In it
Matter arohid flavour, aroma of pure is strong, and soup look is orange, and flavour is mellow.A most important link is a kind of custom in its production process
Claim the process of " floating ", i.e., coronoid process dissipate capsule bacterium is as dominant bacteria, in the raw material of Fu tea --- the vigorous growth in Dark Green Tea.Institute
With, as a kind of microbial fermentation tea (black teas of one of i.e. six big teas), the distinguishing character of Fu tea, peculiar flavor and
Numerous effects are all attributed to the fact that coronoid process dissipate capsule bacterium therein (seeing below document).
[1] Zheng Shujuan, Sheng Yao, Ou little Qun, Xu Wentao, yellow the Kunlun pile fermentation black tea fragrance and primary efficacy progress foods
Product industrial technology, 2016,37 (20)：366-376.
[2] the modest Fu tea coronoid process dissipate capsule bacterium fermenting and producing Yin of Liu Liping, Tang Yuwei, Wang Ruoxian, Li Zhibing, Liu Zhonghua, Liu Shuo
The research tea sciences of diindyl alkaloid, 2017,37 (5)：503-512.
[3] Huang Yan, Shi Rui, the advances and application biological processings for two positive coronoid process dissipate capsule bacteriums of reviving, 2017,15
(1)：49-56.
[4] chemical composition and its Study on functional properties the progress Chinese foods of Hou Aixiang, Li Zong army Fu tea and nutrition,
2016,22 (6)：63-67.
The essence of Dark Green Tea is to experienced the green tea of slight microbial fermentation, bitter taste reduce, flavor more alcohol and, fit
Make various black teas.
The traditional handicraft of production Fu tea is in the form of Dark Green Tea is compressed to brick tea by loose tea or in advance, to be placed in floating storehouse
In library, then under harsh temperature and humidity conditions control, about 2-3 weeks floating process of experience obtains Fu tea finished products.However whether
Actual production still in the lab, we have found to be inoculated with coronoid process dissipate capsule bacterium in Dark Green Tea, and the effect of floating is not
Obviously, the Fu tea of high-quality can not be produced.So how to produce " golden flower " apparent Fu tea, becomes and urgently break through at present
Technical barrier.
Invention content
In order to solve the above technical problem, the present invention provides the application of a kind of floating derivant and the floating derivant with
Fungus growing process.
In order to achieve the object of the present invention, present invention employs following technical schemes：
A kind of floating derivant, the floating derivant include the bacterial strain of bacillus (Bacillussp.), lactobacillus
(Lactobacillussp.) bacterial strain, the lactococcus of bacterial strain, Microbacterium (Microbacteriumsp.)
(Lactococcussp.) in the bacterial strain of bacterial strain and Bifidobacterium (Bifidobacteriumsp.) any one or
Several combination of person.
Further scheme, the freeze-dried processing of the floating derivant obtain.
Further scheme is added with coronoid process dissipate capsule bacterium (Eurotiumcristatum) in the floating derivant.
Further scheme, the freeze-drying process process are：Milk is subjected to centrifugal treating and removes upper layer grease, later
It carries out sterilization treatment and obtains spare protection agent solution；Then 5 to 7 days coronoid process dissipate capsule bacterium institutes will be grown in PDA culture medium
The spore of generation, and the floating derivant bacterium bacterial strain cultivated 48 hours on LB or MRS culture mediums are suspended in the guarantor respectively
It protects in agent solution, after 2~5 hours to solid-state of -80 DEG C of pre-freezes, is put into freeze drier and carries out vacuum freeze drying 15~20
Hour.
Application of the floating derivant in tealeaves floating process a kind of described in.
A kind of fungus growing process using the floating derivant includes the following steps：
Step 1, coronoid process dissipate capsule bacterium and floating derivant are added：By the spore suspension of the coronoid process dissipate capsule bacterium and described
The bacteria suspension of floating derivant is added in the tea raw material for waiting for floating；
Step 2, floating：Floating 3~5 days under the conditions of the tea raw material is placed in 28~32 DEG C obtain the production of floating type tea
Product.
Preferable scheme is that before addition coronoid process dissipate capsule bacterium and floating derivant, first go out to all kinds of tea raw materials
Bacterium is handled.
Further scheme, the preparation method of the spore suspension of coronoid process dissipate capsule bacterium is in the step 1：First by coronoid process dissipate capsule bacterium
It is activated with PDA culture medium, then sterile water is added on the PDA culture medium surface, spore is gently scraped with cotton swab and is made one
Determine the coronoid process dissipate capsule bacterium spore aqueous suspensions of concentration；
The preparation method of the bacteria suspension of floating derivant is in the step 1：By the floating derivant LB culture mediums
Or MRS culture mediums are activated, and corresponding bacteria suspension is made with sterile water mixing in the floating derivant later.
Further scheme, additive amount of the spore suspension of coronoid process dissipate capsule bacterium in tealeaves is in the step 1：Every 28~32
The spore suspension of 1 milliliter of coronoid process dissipate capsule bacterium is added in gram tealeaves；Additive amount of the floating derivant bacteria suspension in tealeaves be：
1 milliliter of bacteria suspension is added in every 28~32 grams of tealeaves.
Further scheme, the tea raw material in the step 1 are green tea, white tea, yellow tea, oolong tea, black tea and Dark Green Tea
Any one of.
The beneficial effects of the present invention are：
(1) present invention discover that there are a kind of quorum sensing mechanisms between the coronoid process dissipate capsule bacterium and the floating derivant.
That is the raised growth of floating derivant so that certain bacterium metabolite is had accumulated in its ambient enviroment, when this metabolin exists
It when concentration in environment reaches certain threshold value, is just identified by coronoid process dissipate capsule bacterium, to promote the vigorous growth of coronoid process dissipate capsule bacterium.
This is not only greatly improved floating effect, has also significantly speeded up floating speed.
The floating derivant in the present invention is the raw material for producing high-quality floating type tea product.The floating lures
Leading agent can solve that Fu tea and other floating type tea product process floating periods are long, floating quality is unstable and may go out
The problems such as existing harmful miscellaneous bacteria, i.e., the described floating derivant can not only improve floating efficiency, enhancing floating effect, but also have specific micro-
Bio-safety ensures, can provide comprising the prebiotic Fu tea of one or several kinds of beneficial microorganism high-quality and other floating type benefits
Raw tea product.
(2) product under the lyophilised state of long-term preservation can be made in floating derivant of the present invention, convenient for directly making
With.The product, which can be inoculated in after directly being mixed with sterile water in tealeaves, is used for floating.
(3) coronoid process dissipate capsule bacterium and floating derivant provided by the invention are combined, to green tea, white tea, yellow tea, oolong tea, red
Tea and Dark Green Tea can have extraordinary promotion floating effect.The present invention can be on the basis for the organoleptic features for retaining major teas
On, the peculiar flavour of its " golden flower " is further assigned, all kinds of novel floating type teas are produced.The technique is existing major teas
Wide development space is provided in terms of the further exploitation of safe efficient, the prebiotic healthcare function of tool floating type tea product.
(4) fungus growing process provided by the invention can occur being harmful to miscellaneous bacteria first to avoid tea processing process, secondly can be with
Tea raw material is set to realize that a large amount of hair time spents are shortened to by 2 to 3 original weeks within 5 days.Moreover, the floating induction
Agent is probiotic strain composition, can be that tea product introduces new health-care efficacy.Present invention process is simple, can produce a variety of hairs
Flower pattern health care tea product.
Description of the drawings
Fig. 1 is white tea after irradiation sterilization in different microorganisms addition treated the 5th day floating situation comparison.
Fig. 2 is Dark Green Tea after irradiation sterilization in different microorganisms addition treated the 5th day floating situation comparison.
Fig. 3 is white tea after high pressure steam sterilization in different microorganisms addition treated the 5th day floating situation comparison.
Fig. 4 is oolong tea after high pressure steam sterilization in different microorganisms addition treated the 5th day floating situation pair
Than.
Fig. 5 is green tea after high pressure steam sterilization in different microorganisms addition treated the 5th day floating situation comparison.
Fig. 6 is yellow tea after high pressure steam sterilization in different microorganisms addition treated the 5th day floating situation comparison.
Fig. 7 is black tea after high pressure steam sterilization in different microorganisms addition treated the 5th day floating situation comparison.
Fig. 8 is Dark Green Tea after high pressure steam sterilization in different microorganisms addition treated the 5th day floating situation pair
Than.
Fig. 9 is that treated the 5th in the different microorganisms of addition lyophil preservation for Dark Green Tea after high pressure steam sterilization
Its floating situation compares.
Figure 10 is the green tea after high pressure steam sterilization in floating derivant and coronoid process dissipate capsule bacterium addition processing in varing proportions
The 5th day floating situation comparison afterwards.
Figure 11 is oolong tea after high pressure steam sterilization at floating derivant and coronoid process dissipate capsule bacterium in varing proportions addition
The 5th day floating situation comparison after reason.
Figure 12 is Dark Green Tea after high pressure steam sterilization at floating derivant and coronoid process dissipate capsule bacterium in varing proportions addition
The 5th day floating situation comparison after reason.
Figure 13 is the Dark Green Tea after high pressure steam sterilization at floating derivant and coronoid process dissipate capsule bacterium are with different order addition
Floating situation comparison after reason.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, to the technical solution in the present invention carry out it is clear, completely retouch
It states.Following embodiment is used for illustrating the present invention, but is not limited to the scope of the present invention.
In microbial strains provided by the invention, coronoid process dissipate capsule bacterium, hay bacillus, moth microbacterium are isolated from commercially available Fu
Tea, cultivar identification are based on DNA comparison results.Other 5 plants of bacterium bacterial strains are commercial product.
1 high-quality Fu tea of embodiment and its processing technology
The technique includes the following steps：
It is prepared by tea raw material：Dark Green Tea is taken slightly to be crushed first, next takes a portion to be placed under 9K radiation resistances
Carry out radiation sterilizing；It separately takes a part to be put into high-pressure steam sterilizing pan to sterilize 20 minutes in 121 DEG C, then in gnotobasis condition
Under, tea raw material that above two sterilization method obtains is added to 12 porocyte culture plates with the amount in about 0.6 gram of every hole respectively
In, for use.
The preparation of eurotium cristatum spore suspension：First coronoid process dissipate capsule bacterium is fully activated in culture dish until its generation is big
Spore is measured, then sterile water is added in its media surface, coronoid process dissipate capsule bacterium spore aqueous suspensions are made with cotton swab scraping spore
(600CFU/mL, this concentration is not required to considered critical, any to be similar to or may each be suitable concentration higher than the concentration of the value),
For use.
The preparation of floating derivant bacteria suspension：The hay bacillus of bacillus, bacillus coagulans, methyl are sought respectively
The type of supporting bacillus, the lactobacillus delbruockii subspecies bulgaricus of lactobacillus, the Lactococcus lactis of lactococcus, Bifidobacterium
Bifidobacterium adolescentis and 7 bacterial strains such as moth microbacterium of Microbacterium activated, certain density phase is made with sterile water
The bacteria suspension (10 of the single plant bacterium answered6CFU/mL), title is demarcated, for use.The bacteria suspension of this 7 bacterial strains is taken one respectively again
Divide and be used as a kind of mixed cell bacteria suspension by equal proportion mixing, demarcates title, for use.
The preparation of coronoid process dissipate capsule bacterium and floating derivant bacterium solution can also be：Take the coronoid process dissipate capsule bacterium spore of lyophil preservation
The hay bacillus of son and bacillus, bacillus coagulans, Methylotrophic bacillus, lactobacillus De Shi breast bars
Bacterium subspecies bulgaricus, the Lactococcus lactis of lactococcus, the moth of the bifidobacterium adolescentis of Bifidobacterium and Microbacterium are micro-
The strain (being about 0.05 gram) of bacillus uses 1mL sterile water mixings, corresponding coronoid process dissipate capsule bacterium and each floating is made respectively
The bacteria suspension of derivant freeze-dried powder.In this operating method, first fungal spore and the freeze-dried powder of bacterium are mixed with sterile water respectively
It is outstanding to remix, or first the spore of coronoid process dissipate capsule bacterium and the freeze-dried powder of bacterium are mixed and are suspended again with sterile water, it is former that tea is added
It is the same to promote the effect of floating after material.
By the above-mentioned eurotium cristatum spore suspension prepared with volume and mass ratio (ml/g) about 1:30 ratio
(can be adjusted accordingly herein according to practical application in production) is added on the sterile tea raw material of above-mentioned 12 porocyte culture plates, then
By the bacteria suspension of single plant bacterium or mixed cell bacteria suspension with 106CFU/mL or 105CFU/mL、104CFU/mL、103CFU/
mL、102The bacterial concentration of CFU/mL, with volume and mass ratio (ml/g) about 1:30 ratio is added on above-mentioned tea raw material.
Next the tea raw material being inoculated with is placed in 30 DEG C of environment, waits for floating process can be completed in about 5 days, the Fu tea of high-quality is made
(see Fig. 2, Fig. 8, Fig. 9, Figure 12, Figure 13).
Here by analyzing attached drawing, which is described further：
First, comparison diagram 2, Fig. 8 be visible irradiated or the Dark Green Tea raw material of the methods of high-pressure steam sterilizing can be smoothly
Floating, and the floating control group with obvious effects better than no floating derivant, so the method for sterilizing is not to limit, i.e., it is various
Sterilizing methods should all can be as the pre-treating method of floating tea raw material.
Microorganism addition type is respectively in each hole of tissue culture plate in Fig. 2：
C2：Coronoid process dissipate capsule bacterium；C3：It is not added with microorganism；A1：Coronoid process dissipate capsule bacterium+hay bacillus；A2：Coronoid process dissipate capsule bacterium+first
Base auxotype bacillus；A3：Coronoid process dissipate capsule bacterium+bacillus coagulans；A4：Coronoid process dissipate capsule bacterium+moth microbacterium；B1：Coronoid process dissipates
Capsule bacterium+Bulgarian Lactobacillus；B2：The bacteria suspension of coronoid process dissipate capsule bacterium+above 5 bacterium bacterial strain equal proportions mixing.
From figure 2 it can be seen that using the Dark Green Tea that sterilizes as tea raw material, this 5 kinds of probiotics floating derivants are added or it is mixed
Coronoid process dissipate capsule bacterium floating can be obviously promoted after combined bacteria agent.
Secondly, comparison diagram 8 is visible with Fig. 9 with the microorganism fungus kind activated temporarily, or with the microbial bacteria being lyophilized in advance
Powder can make the smooth floating of Dark Green Tea, and floating effect is equally substantially better than the control group of no floating derivant, so microorganism
The addition form of strain is also not limit, that is, the microorganism fungus kind activated temporarily is added or the microorganism of freeze-drying preservation is added
Strain can make Dark Green Tea or even other a variety of smooth floatings of tea raw material.
Microorganism addition type is respectively in each hole of tissue culture plate in Fig. 8：
C2：Coronoid process dissipate capsule bacterium；C4：It is not added with microorganism；A1：Coronoid process dissipate capsule bacterium+hay bacillus；A3：Coronoid process dissipate capsule bacterium+breast
Acid streptococci；A4：Coronoid process dissipate capsule bacterium+moth microbacterium；B1：Coronoid process dissipate capsule bacterium+Methylotrophic bacillus；B2：Coronoid process dissipates capsule
Bacterium+bacillus coagulans；B4：Coronoid process dissipate capsule bacterium+Bulgarian Lactobacillus.
As can be seen from Figure 8, this 6 kinds of bacterium floating derivants provided by the invention can be obviously promoted coronoid process dissipate capsule bacterium
Floating in the Dark Green Tea raw material that sterilizes.
Microorganism addition type is respectively in each hole of tissue culture plate in Fig. 9：
C2：Coronoid process dissipate capsule bacterium；C3：It is not added with microorganism；A1：Coronoid process dissipate capsule bacterium+hay bacillus；A2：Coronoid process dissipate capsule bacterium+first
Base auxotype bacillus；A3：Coronoid process dissipate capsule bacterium+bacillus coagulans；A4：Coronoid process dissipate capsule bacterium+moth microbacterium；B1：Coronoid process dissipates
Capsule bacterium+streptococcus lactis；B2：Coronoid process dissipate capsule bacterium+bifidobacterium adolescentis；B3：Coronoid process dissipate capsule bacterium+Bulgarian Lactobacillus.
It can be seen in figure 9 that the floating derivant strain of any one lyophil preservation provided by the invention can be with
It is obviously promoted floating of the coronoid process dissipate capsule bacterium in the Dark Green Tea raw material that sterilizes.
Finally, by Figure 12 and Figure 13 as it can be seen that between coronoid process dissipate capsule bacterium and bacterium floating derivant biomass each ratio,
Or the sequencing of addition coronoid process dissipate capsule bacterium and bacterium floating derivant, do not significantly affect floating effect, and its effect is equal
It is significantly better than the control group of no floating derivant.No matter this proves suitable with which kind of between coronoid process dissipate capsule bacterium and bacterium floating derivant
Tea raw material is added in sequence or ratio, can generate good floating effect.
Microorganism addition type is respectively in each hole of tissue culture plate in Figure 12：
A1：Coronoid process dissipate capsule bacterium (600CFU/mL)+hay bacillus (106CFU/mL)A2：Coronoid process dissipate capsule bacterium (600CFU/mL)+
Hay bacillus (105CFU/mL)；A3：Coronoid process dissipate capsule bacterium (600CFU/mL)+hay bacillus (104CFU/mL)；A4：Coronoid process dissipate capsule bacterium
(600CFU/mL)+hay bacillus (103CFU/mL)；B1：Coronoid process dissipate capsule bacterium (600CFU/mL)+hay bacillus (100CFU/mL)；
B2：Coronoid process dissipate capsule bacterium (600CFU/mL)；B3：It is not added with microorganism.
In figure 12 it can be seen that floating derivant strain provided by the invention is being added with coronoid process dissipate capsule bacterium with arbitrary proportion
The case where adding, can be obviously promoted floating of the coronoid process dissipate capsule bacterium in the Dark Green Tea raw material that sterilizes.
Microorganism addition type is respectively in each hole of tissue culture plate in Figure 13：
A1：Coronoid process dissipate capsule bacterium is first added, lactobacillus bulgaricus strain is added after 48 hours, and (A1a, A1b, A1c distinguish
To be added the 2nd day, the 3rd day, the 4th day floating picture of Bulgarian Lactobacillus).
A2：Coronoid process dissipate capsule bacterium is first added, B. subtilis strain is added after 48 hours, and (A2a, A2b, A2c are respectively added withered
The 2nd day of straw bacterium, the 3rd day, the 4th day floating picture).
B1：Lactobacillus bulgaricus strain is first added, coronoid process dissipate capsule bacterium is added after 48 hours, and (B1a, B1b, B1c distinguish
To be added the 2nd day, the 3rd day, the 4th day floating picture of coronoid process dissipate capsule bacterium).
B2：B. subtilis strain is first added, coronoid process dissipate capsule bacterium is added after 48 hours, and (B2a, B2b, B2c are respectively that hat is added
The 2nd day, the 3rd day, the 4th day floating picture of prominent bulk bacteria).
C1：Coronoid process dissipate capsule bacterium and lactobacillus bulgaricus strain (C1a, C1b, C1c respectively two classes of addition are added simultaneously
The 4th day of microorganism, the 5th day, the 6th day floating picture).
C2：Coronoid process dissipate capsule bacterium and B. subtilis strain are added simultaneously, and (C2a, C2b, C2c are respectively that two quasi-microorganisms are added
4th day, the 5th day, the 6th day floating picture).
B4：Only addition coronoid process dissipate capsule bacterium (B4a, B4b, B4c are respectively to be added the 2nd day of coronoid process dissipate capsule bacterium, the 3rd day, the 4th
Its floating picture).
C4：It is not added with microorganism (C4a, C4b, C4c are respectively the 4th day, the 5th day, the 6th day picture).
As seen from Figure 13, no matter how coronoid process dissipate capsule bacterium and the order of addition of bacteria-induction agent change, tea raw material
Notable floating appears at after coronoid process dissipate capsule bacterium and floating derivant co-inoculation about 5 days or so, and floating effect is notable
Better than the control group of no derivant.So floating derivant strain provided by the invention and coronoid process dissipate capsule bacterium are added in various orders
Floating of the latter in the Dark Green Tea raw material that sterilizes can be obviously promoted.
2 high-quality floating white tea of embodiment and its processing technology
The technique includes the following steps：
It is prepared by tea raw material：Take white tea slightly to be crushed first, next take a portion be placed under 9K radiation resistances into
Row radiation sterilizing；It separately takes a part to be put into high-pressure steam sterilizing pan to sterilize 20 minutes in 121 DEG C, then in gnotobasis condition
Under, tea raw material that above two sterilization method obtains is added to 12 porocyte culture plates with the amount in about 0.6 gram of every hole respectively
In, for use.
The preparation of eurotium cristatum spore suspension：With embodiment 1.
The preparation of floating derivant bacteria suspension：With embodiment 1.
By the above-mentioned eurotium cristatum spore suspension prepared with volume and mass ratio (ml/g) about 1:30 ratio
(can be adjusted accordingly herein according to practical application in production) is added on the sterile tea raw material of above-mentioned 12 porocyte culture plates, then
Single plant bacterium bacteria suspension or mixed cell bacteria suspension are added to ratio identical with spore suspension on above-mentioned tea raw material, connect down
The tea raw material being inoculated with is placed in 30 DEG C of environment, waits for floating process can be completed in about 5 days, the floating white tea of high-quality is made
(see Fig. 1 and Fig. 3).
The case where to embodiment 1, is similar, we pass through analysis chart 1 and Fig. 3, equally visible irradiated or high-pressure steam etc.
The smooth floating of energy of the white tea raw material of method sterilizing, and the floating control group with obvious effects better than no floating derivant.
Microorganism addition type is respectively in each hole of tissue culture plate in Fig. 1：
C1：Coronoid process dissipate capsule bacterium；C3：It is not added with microorganism；A1：Coronoid process dissipate capsule bacterium+hay bacillus；A2：Coronoid process dissipate capsule bacterium+first
Base auxotype bacillus；A3：Coronoid process dissipate capsule bacterium+bacillus coagulans；A4：Coronoid process dissipate capsule bacterium+moth microbacterium；B1：Coronoid process dissipates
Capsule bacterium+Bulgarian Lactobacillus；B2：The bacteria suspension of coronoid process dissipate capsule bacterium+above 5 bacterium bacterial strain equal proportions mixing.
From figure 1 it appears that this 5 kinds of bacterium floating derivants provided by the invention and its mix bacterium agent can be apparent
Floating of the coronoid process dissipate capsule bacterium in the white tea raw material that sterilizes is promoted (to pay attention to：The intrinsic a large amount of pekoes of visible white tea in C1 and C3, these
It is not " golden flower ".)
Microorganism addition type is respectively in each hole of tissue culture plate in Fig. 3：
C3：Coronoid process dissipate capsule bacterium；C4：It is not added with microorganism；A1：Coronoid process dissipate capsule bacterium+hay bacillus；A3：Coronoid process dissipate capsule bacterium+breast
Acid streptococci；A4：Coronoid process dissipate capsule bacterium+moth microbacterium；B1：Coronoid process dissipate capsule bacterium+Methylotrophic bacillus；B2：Coronoid process dissipates capsule
Bacterium+bacillus coagulans；B3：Coronoid process dissipate capsule bacterium+bifidobacterium adolescentis；B4：Coronoid process dissipate capsule bacterium+Bulgarian Lactobacillus；C1：
The bacteria suspension of coronoid process dissipate capsule bacterium+above 7 bacterium bacterial strain equal proportions mixing.
From figure 3, it can be seen that the floating derivant and its mix bacterium agent of the present invention, which can be obviously promoted coronoid process, dissipates capsule
Floating of the bacterium in the white tea raw material that sterilizes (pays attention to：The intrinsic a large amount of pekoes of visible white tea in C3 and C4, these are not " golden flower ".)
Examples 1 and 2 can prove that various sterilizing methods should all can be as the pre-treating method of floating tea raw material.
3 high-quality floating oolong tea of embodiment and its processing technology
The technique includes the following steps：
It is prepared by tea raw material：Oolong tea is taken slightly to be crushed first, next puts it into 121 in high-pressure steam sterilizing pan
DEG C, it sterilizes 20 minutes, then under the conditions of gnotobasis, is added in 12 porocyte culture plates with the amount of every hole about 0.6g, for use.
The preparation of eurotium cristatum spore suspension：With embodiment 1.
The preparation of floating derivant bacteria suspension：With embodiment 1.
By the above-mentioned eurotium cristatum spore suspension prepared with volume and mass ratio (ml/g) about 1:30 ratio
(can be adjusted accordingly herein according to practical application in production) is added on the sterile tea raw material of above-mentioned 12 porocyte culture plates, then
By single plant bacterium bacteria suspension or mixed cell bacteria suspension with 106CFU/mL or 105CFU/mL、104CFU/mL、103CFU/
mL、102The bacterial concentration of CFU/mL, with volume and mass ratio (ml/g) about 1:30 ratio is added on above-mentioned tea raw material,
Next the tea raw material being inoculated with is placed in 30 DEG C of environment, waits for floating process can be completed in about 5 days, the floating of high-quality is made
Oolong tea (see Fig. 4 and Figure 11).
Microorganism addition type is respectively in each hole of tissue culture plate in Fig. 4：
C2：Coronoid process dissipate capsule bacterium；C4：It is not added with microorganism；A1：Coronoid process dissipate capsule bacterium+hay bacillus；A3：Coronoid process dissipate capsule bacterium+breast
Acid streptococci；A4：Coronoid process dissipate capsule bacterium+moth microbacterium；B1：Coronoid process dissipate capsule bacterium+Methylotrophic bacillus；B2：Coronoid process dissipates capsule
Bacterium+bacillus coagulans；B4：Coronoid process dissipate capsule bacterium+Bulgarian Lactobacillus；C1：Coronoid process dissipate capsule bacterium+above 6 bacterium bacterial strains
The bacteria suspension of equal proportion mixing
This 6 kinds of bacterium floating derivants and its mix bacterium agent provided by the invention can be apparent it can be seen from Fig. 4
Promote floating of the coronoid process dissipate capsule bacterium in the oolong tea raw material that sterilizes.
Microorganism addition type is respectively in each hole of tissue culture plate in Figure 11：
A1：Coronoid process dissipate capsule bacterium (600CFU/mL)+hay bacillus (106CFU/mL)；A2：Coronoid process dissipate capsule bacterium (600CFU/mL)
+ hay bacillus (105CFU/mL)；A3：Coronoid process dissipate capsule bacterium (600CFU/mL)+hay bacillus (104CFU/mL)；A4：Coronoid process dissipates capsule
Bacterium (600CFU/mL)+hay bacillus (103CFU/mL)；B1：Coronoid process dissipate capsule bacterium (600CFU/mL)+hay bacillus (100CFU/
mL)；B2：Coronoid process dissipate capsule bacterium (600CFU/mL)；B3：It is not added with microorganism.
Floating derivant strain provided by the invention is being added with coronoid process dissipate capsule bacterium with a variety of ratios it can be seen from Figure 11
Floating of the coronoid process dissipate capsule bacterium in the oolong tea raw material that sterilizes can be obviously promoted in the case of adding.
4 high-quality floating green tea of embodiment and its processing technology
The technique includes the following steps：
It is prepared by tea raw material：Green tea is taken slightly to be crushed first, next puts it into high-pressure steam sterilizing pan 121 DEG C,
Sterilizing 20 minutes, then under the conditions of gnotobasis, be added in 12 porocyte culture plates with the amount of every hole about 0.6g, for use.
The preparation of eurotium cristatum spore suspension：With embodiment 1.
The preparation of floating derivant bacteria suspension：With embodiment 1.
By the above-mentioned eurotium cristatum spore suspension prepared with volume and mass ratio (ml/g) about 1:30 ratio
(can be adjusted accordingly herein according to practical application in production) is added on the sterile tea raw material of above-mentioned 12 porocyte culture plates, then
By floating derivant single plant bacterium bacteria suspension or mixed cell bacteria suspension with 106CFU/mL or 105CFU/mL、104CFU/
mL、103CFU/mL、102The bacterial concentration of CFU/mL, with volume and mass ratio (ml/g) about 1:30 ratio is added to above-mentioned
On tea raw material, next the tea raw material being inoculated with is placed in 30 DEG C of environment, waits for floating process can be completed in about 5 days, height is made
The floating green tea of quality (see Fig. 5 and Figure 10).
Microorganism addition type is respectively in each hole of tissue culture plate in Fig. 5：
C3：Coronoid process dissipate capsule bacterium；C4：It is not added with microorganism；A1：Coronoid process dissipate capsule bacterium+hay bacillus；A3：Coronoid process dissipate capsule bacterium+breast
Acid streptococci；A4：Coronoid process dissipate capsule bacterium+moth microbacterium；B1：Coronoid process dissipate capsule bacterium+Methylotrophic bacillus；B2：Coronoid process dissipates capsule
Bacterium+bacillus coagulans；B3：Coronoid process dissipate capsule bacterium+bifidobacterium adolescentis；B4：Coronoid process dissipate capsule bacterium+Bulgarian Lactobacillus；C1：
The bacteria suspension of coronoid process dissipate capsule bacterium+above 7 bacterium bacterial strain equal proportions mixing.
Any one floating derivant and its mix bacterium agent provided by the invention can be apparent it can be seen from Fig. 5
Promote floating of the coronoid process dissipate capsule bacterium in the green tea materials that sterilize.
Microorganism addition type is respectively in each hole of tissue culture plate in Figure 10：
A1：Coronoid process dissipate capsule bacterium (600CFU/mL)+hay bacillus (106CFU/mL)；A2：Coronoid process dissipate capsule bacterium (600CFU/mL)
+ hay bacillus (105CFU/mL)；A3：Coronoid process dissipate capsule bacterium (600CFU/mL)+hay bacillus (104CFU/mL)；A4：Coronoid process dissipates capsule
Bacterium (600CFU/mL)+hay bacillus (103CFU/mL)；B1：Coronoid process dissipate capsule bacterium (600CFU/mL)+hay bacillus (100CFU/
mL)；B2：Coronoid process dissipate capsule bacterium (600CFU/mL)；B3：It is not added with microorganism.
Floating derivant strain provided by the invention is being added with coronoid process dissipate capsule bacterium with a variety of ratios it can be seen from Figure 10
Floating of the coronoid process dissipate capsule bacterium in the green tea materials that sterilize can be obviously promoted in the case of adding.
Pass through analysis chart 10, Figure 11 and Figure 12, it has been found that between coronoid process dissipate capsule bacterium and bacterium floating derivant 5 across
Several apparent floatings of tea raw material can be made by spending under prodigious biomass ratio, and its effect is significantly better than no floating induction
The control group of agent.No matter which kind of ratio tea raw material is added between provable coronoid process dissipate capsule bacterium and bacterium floating derivant with accordingly,
Good floating effect can be generated.
5 high-quality floating yellow tea of embodiment and its processing technology
The technique includes the following steps：
It is prepared by tea raw material：Yellow tea is taken slightly to be crushed first, next puts it into high-pressure steam sterilizing pan 121 DEG C,
Sterilizing 20 minutes, sterilizing terminate to be cooled to room temperature, then under the conditions of gnotobasis, and it is thin that 12 holes are added with the amount of every hole about 0.6g
In born of the same parents' culture plate, for use.
The preparation of eurotium cristatum spore suspension：With embodiment 1.
The preparation of floating derivant bacteria suspension：With embodiment 1.
By the above-mentioned eurotium cristatum spore suspension prepared with volume and mass ratio (ml/g) about 1:30 ratio
(can be adjusted accordingly herein according to practical application in production) is added on the sterile tea raw material of above-mentioned 12 porocyte culture plates, then
Single plant bacterium bacteria suspension or mixed cell bacteria suspension are added to ratio identical with spore suspension on above-mentioned tea raw material, connect down
The tea raw material being inoculated with is placed in 30 DEG C of environment, waits for floating process can be completed in about 5 days, the floating yellow tea of high-quality is made
(see Fig. 6).
Microorganism addition type is respectively in each hole of tissue culture plate in Fig. 6：
C3：Coronoid process dissipate capsule bacterium；C4：It is not added with microorganism；A1：Coronoid process dissipate capsule bacterium+hay bacillus；A3：Coronoid process dissipate capsule bacterium+breast
Acid streptococci；A4：Coronoid process dissipate capsule bacterium+moth microbacterium；B1：Coronoid process dissipate capsule bacterium+Methylotrophic bacillus；B2：Coronoid process dissipates capsule
Bacterium+bacillus coagulans；B3：Coronoid process dissipate capsule bacterium+bifidobacterium adolescentis；B4：Coronoid process dissipate capsule bacterium+Bulgarian Lactobacillus；C1：
The bacteria suspension of coronoid process dissipate capsule bacterium+above 7 bacterium bacterial strain equal proportions mixing.
Any one floating derivant and its mix bacterium agent provided by the invention can be apparent it can be seen from Fig. 6
Promote floating of the coronoid process dissipate capsule bacterium in the yellow tea raw material that sterilizes.
6 high-quality floating black tea of embodiment and its processing technology
The technique includes the following steps：
It is prepared by tea raw material：Black tea is taken slightly to be crushed first, next puts it into high-pressure steam sterilizing pan 121 DEG C,
Sterilizing 20 minutes, sterilizing terminate to be cooled to room temperature, then under the conditions of gnotobasis, and it is thin that 12 holes are added with the amount of every hole about 0.6g
In born of the same parents' culture plate, for use.
The preparation of eurotium cristatum spore suspension：With embodiment 1.
The preparation of floating derivant bacteria suspension：With embodiment 1.
By the above-mentioned eurotium cristatum spore suspension prepared with volume and mass ratio (ml/g) about 1:30 ratio
(can be adjusted accordingly herein according to practical application in production) is added on the sterile tea raw material of above-mentioned 12 porocyte culture plates, then
Single plant bacterium bacteria suspension or mixed cell bacteria suspension are added to ratio identical with spore suspension on above-mentioned tea raw material, connect down
The tea raw material being inoculated with is placed in 30 DEG C of environment, waits for floating process can be completed in about 5 days, the floating black tea of high-quality is made
(see Fig. 7).
Microorganism addition type is respectively in each hole of tissue culture plate in Fig. 7：
C2：Coronoid process dissipate capsule bacterium；C4：It is not added with microorganism；A1：Coronoid process dissipate capsule bacterium+hay bacillus；A2：Coronoid process dissipate capsule bacterium+first
Base auxotype bacillus；A3：Coronoid process dissipate capsule bacterium+bacillus coagulans；A4：Coronoid process dissipate capsule bacterium+moth microbacterium；B1：Coronoid process dissipates
Capsule bacterium+Bulgarian Lactobacillus；B2：Coronoid process dissipate capsule bacterium+streptococcus lactis；B3：Coronoid process dissipate capsule bacterium+bifidobacterium adolescentis；B4：
The bacteria suspension of coronoid process dissipate capsule bacterium+above 7 kinds of bacterium bacterial strain equal proportions mixing.
Any one floating derivant and its mix bacterium agent provided by the invention can be apparent it can be seen from Fig. 7
Promote floating of the coronoid process dissipate capsule bacterium in the black tea raw material that sterilizes.
The floating temperature of above-mentioned each technique be laboratory condition under set temperature, in production when can be according to actual environment item
Part adjusts accordingly.
In summary embodiment is found：Bacillus bacterial strain, Lactobacillus bacteria bacterial strain, Microbacterium bacterium bacterium
Strain, lactococcus bacteria bacterial strain, bifidobacterium bacteria bacterial strain, can respectively by individually or using arbitrary proportion mix in the form of as
Floating derivant and coronoid process dissipate capsule bacterium are used in combination, and coronoid process dissipate capsule bacterium can be promoted efficiently to send out applied to tea leaf fermentation manufacture field
Flower, greatly improves the production efficiency of floating type tea product, and improve sample tea entirety flavor.In addition, since tea raw material passes through
Sterilization treatment is crossed, and derivant is probiotic bacterial strain, also significantly increases safety and the tea product of tea leaf fermentation process
Health-care efficacy.
Claims (10)
1. a kind of floating derivant, it is characterised in that：The floating derivant includes the bacterium of bacillus (Bacillus sp.)
Strain, the bacterial strain of lactobacillus (Lactobacillus sp.), Microbacterium (Microbacterium sp.) bacterial strain, milk-globule
It is arbitrary in the bacterial strain of Pseudomonas (Lactococcus sp.) and the bacterial strain of Bifidobacterium (Bifidobacterium sp.)
One or several kinds of combinations.
2. floating derivant as described in claim 1, it is characterised in that：Coronoid process dissipate capsule bacterium is added in the floating derivant
(Eurotium cristatum)。
3. floating derivant as claimed in claim 1 or 2, it is characterised in that：The freeze-dried processing of floating derivant
It obtains.
4. floating derivant as claimed in claim 3, it is characterised in that：The freeze-drying process process is：By milk into
Row centrifugal treating removes upper layer grease, carries out sterilization treatment later and obtains spare protection agent solution；It then will be in PDA culture medium
Spore caused by 5 to 7 days coronoid process dissipate capsule bacteriums is grown, and grows the floating of 48 hours on LB or MRS culture mediums and lures
It leads agent bacterium bacterial strain to be suspended in respectively in the protection agent solution, after 2~5 hours to solid-state of -80 DEG C of pre-freezes, is put into freezing
Drying machine carries out vacuum freeze drying 15~20 hours.
5. a kind of such as application of the Claims 1 to 4 any one of them floating derivant in tealeaves floating process.
6. a kind of fungus growing process using floating derivant described in claim 1, it is characterised in that include the following steps：
Step 1, coronoid process dissipate capsule bacterium and floating derivant are added：By the spore suspension of the coronoid process dissipate capsule bacterium and the floating
The bacteria suspension of derivant is added in the tea raw material for waiting for floating；
Step 2, floating：Floating 3~5 days under the conditions of the tea raw material is placed in 28~32 DEG C obtain floating type tea product.
7. the fungus growing process of floating derivant as claimed in claim 6, it is characterised in that：In addition coronoid process dissipate capsule bacterium and hair
Before flower derivant, sterilization treatment first is carried out to all kinds of tea raw materials.
8. the fungus growing process of floating derivant as claimed in claim 6, it is characterised in that：
The preparation method of the spore suspension of coronoid process dissipate capsule bacterium is in the step 1：First coronoid process dissipate capsule bacterium is carried out with PDA culture medium
Activation, then sterile water is added on the PDA culture medium surface, spore is gently scraped with cotton swab, and the scattered capsule of certain density coronoid process is made
Bacterium spore aqueous suspensions；
The preparation method of the bacteria suspension of floating derivant is in the step 1：By floating derivant LB culture mediums or the MRS
Culture medium is activated, and corresponding bacteria suspension is made with sterile water mixing in the floating derivant later.
9. the fungus growing process of floating derivant as claimed in claim 6, it is characterised in that：Coronoid process dissipate capsule bacterium in the step 1
Additive amount of the spore suspension in tealeaves be：The spore suspension of 1 milliliter of coronoid process dissipate capsule bacterium is added in every 28~32 grams of tealeaves；Institute
Stating additive amount of the floating derivant bacteria suspension in tealeaves is：1 milliliter of bacteria suspension is added in every 28~32 grams of tealeaves.
10. the fungus growing process of floating derivant as claimed in claim 6, it is characterised in that：Floating type tea is former in the step 1
Material is any one of green tea, white tea, yellow tea, oolong tea, black tea and Dark Green Tea.
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