CN108633914A - A kind of bacteria agent for preventing Pestalotiopsis photiniae - Google Patents
A kind of bacteria agent for preventing Pestalotiopsis photiniae Download PDFInfo
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- CN108633914A CN108633914A CN201810292242.0A CN201810292242A CN108633914A CN 108633914 A CN108633914 A CN 108633914A CN 201810292242 A CN201810292242 A CN 201810292242A CN 108633914 A CN108633914 A CN 108633914A
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- strawberry
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- microbial bacterial
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/34—Nitriles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The present invention relates to a kind of microbial bacterial agents of prevention Strawberry Root Rot, are characterized in that：The microbial bacterial agent includes dwell rice pseudomonad bacterium solution and synergist；The rice pseudomonad that dwells, depositary institution：China Committee for Culture Collection of Microorganisms's common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, deposit number is CGMCC No.15325, the deposit date is on 01 29th, 2018, synergist was 4,000 5000 liquid mixed liquor of Bravo and hymexazol；The bacterial manure is good in rhizosphere of strawberry colonization ability and safe and reliable, and there is good degradation to pine root fungus in continuous cropping strawberry soil, soil physico-chemical property is significantly improved, the anti-adversity ability of strawberry is promoted, improves the yield and fruit quality of strawberry.
Description
Technical field
The present invention relates to field of biological control, and in particular to a kind of bacteria agent for preventing Pestalotiopsis photiniae.
Background technology
Strawberry Root Rot is mainly caused harm root system disease, when morbidity by tiny lateral root or new root, just there is light red
The irregular patch of brown, color are gradually deepened in crineous.Develop with disease, all root systems downright bad browning rapidly.Overground part
Point it is the jaundice of siphonal lobe leaf margin, browning, necrosis to crispaturaing, diseased plant shows water shortage shape, gradual centripetal to be developed to the withered and yellow death of complete stool.Grass
Certain kind of berries root rot plant root is shorter and smaller than healthy plant root system, and color is gloomy, and underground part adventitious root mortality, new root is by disease
The infringement of opportunistic pathogen, growth are sparse.Root is aggrieved, and absorbability declines, and causes moisture, inorganic matter and nutriment normal
Conveying, causes overground part small and weak or whole strain blueness is withered.
Strawberry Root Rot is the general name of a major class disease caused by a variety of pathogens and environmental interaction.Indoor separation
A variety of pathogens can be obtained in old complaint, and common pathogen has：Cause strawberry black root rot mainly has rhizoctonia, sickle-like bacteria, corruption
Mould etc..Harm of the strawberry black root corruption in strawberry root disease in recent years is most heavy, influences maximum, and pathogen is related to more than ten of category,
It wherein further include pathogenic nematode class.For strawberry black root rot because of complexity, Disease Cycle is various.It can be by a variety of pathogenic soil fungies, line
The barren and unfavorable environment of worm, soil property（Such as freeze injury）Caused by independent or compound.Hebei strawberry base strawberry black root corruption root all over the city
The main pathogen object that portion's tissue is separated to has Rhizoctonia solani Kuhn etc..
For strawberry cultivating peasant household, in the 3-4 of strawberry picking, frequent crop rotation can cause economic benefit to reduce, and
It is be easy to cause using the method for fertilising and hinders the adverse consequences such as seedling, single chemical control can cause under drug resistance and strawberry quality
Drop.
Invention content
In order to make up above-mentioned the deficiencies in the prior art, the present invention proposes a kind of biological bacteria for preventing Pestalotiopsis photiniae
Agent, for preventing Strawberry Root Rot, while the suppression harmful bacteria in Strawberry Root Exudates of degrading, prevention soil surface characters are played,
Promote the purpose of strawberry growth and degradation Auto toxicity.
The purpose of the present invention is what is be achieved through the following technical solutions, a kind of microbial bacterial agent of prevention Strawberry Root Rot,
It is characterized in that：The microbial bacterial agent includes dwell rice pseudomonad bacterium solution and synergist；
The rice pseudomonad that dwells（Pseudomonas oryzihabitans）, depositary institution：Chinese microorganism strain preservation
Administration committee's common micro-organisms center, address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences microorganism
Research institute, deposit number are CGMCC No. 15325, and the deposit date is on 01 29th, 2018；
The bacterium colony and thalline of the bacterial strain are characterized as：The suppression harmful bacteria degradation bacteria is in NA culture mediums（Yeast extract 1g；Beef extract 3g；
Peptone 5g；Sucrose 10g；Agar 18g；Distilled water 1000ml；121 DEG C of sterilizing 30min）After upper culture 48h bacterium colony it is smooth or
Wrinkle, neat in edge or irregular, plano-convex is faint yellow, and colony diameter 2mm after 30 DEG C of culture 2d, thalline is rod-shaped, flagellum fortune
It is dynamic, do not produce gemma, Gram-negative；
The bacterial strain physiological and biochemical property is：Oxidase test is negative, catalase test is positive, methyl red test is negative, starch water
Solve negative, gelatin hydrolysis negative, the nitrate reduction test positive, H2S negatives, malonate utilization test sun
Property, citrate is positive using experiment, glucose utilization experiment positive, the experiment of lactose utilization negative, galactose utilization are positive
Property, maltose it is positive using experiment.
The optimum culture medium for rice pseudomonad strain of dwelling is LB culture mediums, and formula includes that tryptone 10g/L, yeast carry
Take object 5g/L, sodium chloride（NaCl）10g/L, inoculum concentration are the 2% of culture medium weight, and fermentation temperature is 28 DEG C, and mixing speed is
120rpm, fermentation time are 5 days.
The rice pseudomonad that dwells prepares a concentration of 2.5-3.5 × 10 of microbial bacterial agent8cfu/ml；
Synergist is Bravo and hymexazol 4000-5000 liquid mixed liquors, and the ratio of the two is 1:8-8:1.
The method of application of microbial bacterial agent：Root irrigation, the rice pseudomonad that dwells is used to prepare micro- life in strawberry root
Object bacteria suspension concentration is 2.0-3.0 × 108The ratio of cfu/ml, Bravo and hymexazol 4000-5000 liquid mixed liquors, the two is 1:
8-8:1。
Beneficial effects of the present invention：The bacterial manure is good in rhizosphere of strawberry colonization ability and safe and reliable, and to continuous cropping
Pine root fungus has good degradation in strawberry soil, hence it is evident that improves soil physico-chemical property, promotes strawberry
Anti-adversity ability improves the yield and fruit quality of strawberry；Preparation method is simple, and formula is reasonable；While strawberry root system of degrading
Suppression harmful bacteria in secretion plays prevention soil surface characters, promotes the purpose of strawberry growth and degradation Auto toxicity.
Specific implementation mode
Embodiment 1：
The present embodiment provides a kind of bacteria agent of effective prevention Strawberry Root Rot, while alleviating strawberry Auto toxicity and promoting to give birth to
Long development.
1 materials and methods
1.1 material to be tested
For trying strawberry cultivars：Dandong 99
Culture medium for detaching the rice pseudomonad that dwells is minimal medium, and formula includes MgSO4·7H20.2 g/L of O,
CaCl2·2H2O0.02g/L, FeCl30.05g/L, KH2PO41.0 g/L, K2HPO41.0 g/L, NH4NO31.0 g/L, courage
Sterol 0.3 g/L, agar 17g/L are respectively with concentration for the treatment of for 200 μ gmL-1P-hydroxybenzoic acid, benzoic acid and Chinese cassia tree
Acid is used as sole carbon source, adjusts pH value to neutrality.
Method for detaching the rice pseudomonad that dwells：It is cut into segment, 75% alcohol after taking muskmelon rhizome intersection flowing water to rinse
1min, 0.1% mercuric chloride 30s are impregnated, rinsed with sterile water is reused 4 times, grinds and be slurried in sterile mortar, 3 times of grinding fluid dilution
After be coated in minimal medium, cultivate 48 h in 28 DEG C of incubators, obtain rice pseudomonad strain of dwelling.
Dwell rice pseudomonad（Pseudomonas oryzihabitans）, depositary institution：Chinese microorganism strain preservation pipe
Reason committee common micro-organisms center, address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and Chinese Academy of Sciences microorganism is ground
Study carefully institute, deposit number is CGMCC No. 15325, strain number：20180101H16, the deposit date is on 01 29th, 2018.
The bacterium colony and thalline of the bacterial strain are characterized as：The suppression harmful bacteria degradation bacteria is in NA culture mediums（Yeast extract 1g；Beef extract
3g；Peptone 5g；Sucrose 10g；Agar 18g；Distilled water 1000ml；121 DEG C of sterilizing 30min）Bacterium colony is smooth after upper culture 48h
Or wrinkle, neat in edge or irregular, plano-convex is faint yellow, and colony diameter 2mm after 30 DEG C of culture 2d, thalline is rod-shaped, flagellum fortune
It is dynamic, do not produce gemma, Gram-negative.
The bacterial strain physiological and biochemical property is：Oxidase test is negative, catalase test is positive, methyl red test is negative, forms sediment
Powder hydrolysis experiment feminine gender, gelatin hydrolysis negative, the nitrate reduction test positive, H2S negatives, malonate utilize examination
Testing positive, citrate, using experiment, positive, glucose utilization tests the positive, lactose utilization negative, galactose utilization try
It is positive using experiment to test positive, maltose.
The optimum culture medium for rice pseudomonad strain of dwelling is LB culture mediums, and formula includes that tryptone 10g/L, yeast carry
Take object 5g/L, sodium chloride（NaCl）10g/L, inoculum concentration are the 2% of culture medium weight, and fermentation temperature is 28 DEG C, and mixing speed is
120rpm, fermentation time are 5 days.
Prepare bacteria agent：The rice pseudomonad that dwells prepares microbial bacterial agent a concentration of 3.0 × 108cfu/ml；Synergist is
The ratio of 4000 liquid mixed liquor of Bravo and hymexazol, the two is 1:1.
50% Bravo wettable powder（Jiangyin Fuda agrochemical Co., Ltd produces）
99% hymexazol active compound（The oasis Yan Bian Chemical Co., Ltd.）
The method of application of microbial bacterial agent：Root irrigation is used in strawberry root.
1.2 test method
Strawberry transplanting is front and back to carry out pouring root with microbial bacterial agent 200ml.
2 test results
Test result shows bacteria agent pouring root using the present invention, hence it is evident that the incidence for reducing Strawberry Root Rot improves simultaneously
Yield of strawberry.
1 Strawberry Root Rot of table prevents field test results
| Processing | Root rot incidence | Per mu yield（kg） |
| Bacteria agent root irrigation | 0.28% | 1152 |
| Non- root irrigation | 2.3% | 893 |
Claims (2)
1. a kind of microbial bacterial agent of prevention Strawberry Root Rot, it is characterized in that：The microbial bacterial agent includes the rice pseudomonad that dwells
Bacterium solution and synergist；
The rice pseudomonad that dwells（Pseudomonas oryzihabitans）, depositary institution：Chinese microorganism strain preservation
Administration committee's common micro-organisms center, address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences microorganism
Research institute, deposit number are CGMCC No. 15325, and the deposit date is on 01 29th, 2018；
The bacterium colony and thalline of the bacterial strain are characterized as：The suppression harmful bacteria degradation bacteria is in NA culture mediums（Yeast extract 1g；Beef extract 3g；
Peptone 5g；Sucrose 10g；Agar 18g；Distilled water 1000ml；121 DEG C of sterilizing 30min）After upper culture 48h bacterium colony it is smooth or
Wrinkle, neat in edge or irregular, plano-convex is faint yellow, and colony diameter 2mm after 30 DEG C of culture 2d, thalline is rod-shaped, flagellum fortune
It is dynamic, do not produce gemma, Gram-negative；
The bacterial strain physiological and biochemical property is：Oxidase test is negative, catalase test is positive, methyl red test is negative, starch water
Solve negative, gelatin hydrolysis negative, the nitrate reduction test positive, H2S negatives, malonate utilization test sun
Property, citrate is positive using experiment, glucose utilization experiment positive, the experiment of lactose utilization negative, galactose utilization are positive
Property, maltose it is positive using experiment；
The optimum culture medium for rice pseudomonad strain of dwelling is LB culture mediums, and formula includes tryptone 10g/L, yeast extract
5g/L, sodium chloride（NaCl）10g/L, inoculum concentration are the 2% of culture medium weight, and fermentation temperature is 28 DEG C, and mixing speed is
120rpm, fermentation time are 5 days；
The rice pseudomonad that dwells prepares a concentration of 2.5-3.5 × 10 of microbial bacterial agent8cfu/ml；
Synergist is Bravo and hymexazol 4000-5000 liquid mixed liquors, and the ratio of the two is 1:8-8:1.
2. a kind of microbial bacterial agent of prevention Strawberry Root Rot according to claim 1, it is characterized in that：The microorganism
The method of application of microbial inoculum：Root irrigation is used in strawberry root, it is a concentration of that the rice pseudomonad that dwells prepares microbial bacterial agent
2.0-3.0×108Cfu/ml, Bravo and hymexazol（Ratio is 1:1）4000-5000 liquid mixed liquors.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810292242.0A CN108633914A (en) | 2018-04-03 | 2018-04-03 | A kind of bacteria agent for preventing Pestalotiopsis photiniae |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810292242.0A CN108633914A (en) | 2018-04-03 | 2018-04-03 | A kind of bacteria agent for preventing Pestalotiopsis photiniae |
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| CN108633914A true CN108633914A (en) | 2018-10-12 |
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| CN201810292242.0A Withdrawn CN108633914A (en) | 2018-04-03 | 2018-04-03 | A kind of bacteria agent for preventing Pestalotiopsis photiniae |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110041139A (en) * | 2019-05-30 | 2019-07-23 | 沈阳农业大学 | A kind of agent of biological bacteria sandwich tape and application method for continuous cropping soil reparation |
-
2018
- 2018-04-03 CN CN201810292242.0A patent/CN108633914A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110041139A (en) * | 2019-05-30 | 2019-07-23 | 沈阳农业大学 | A kind of agent of biological bacteria sandwich tape and application method for continuous cropping soil reparation |
| CN110041139B (en) * | 2019-05-30 | 2021-11-30 | 沈阳农业大学 | Biological bacterium multilayer tape agent for continuous cropping soil remediation and use method |
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Application publication date: 20181012 |
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