CN108627595B - Method for simultaneously detecting 12 alkaline drugs in hair - Google Patents
Method for simultaneously detecting 12 alkaline drugs in hair Download PDFInfo
- Publication number
- CN108627595B CN108627595B CN201810375411.7A CN201810375411A CN108627595B CN 108627595 B CN108627595 B CN 108627595B CN 201810375411 A CN201810375411 A CN 201810375411A CN 108627595 B CN108627595 B CN 108627595B
- Authority
- CN
- China
- Prior art keywords
- hair
- mobile phase
- simultaneously detecting
- drugs
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention belongs to the technical field of drug detection, and particularly relates to a method for simultaneously detecting 12 alkaline drugs in hair. The method for simultaneously analyzing the 12 psychotropic drugs in the hair is simple, convenient and rapid, and has wide analysis range. Compared with the existing method, the method omits the liquid-liquid extraction process in the sample pretreatment, the whole process can be completed within 1h, and the time is not increased; in contrast, when multiple samples are analyzed, the advantage of high throughput is more pronounced. The technical scheme provided by the invention can realize external pollution detection and instant quantitative analysis of actual cases, and is convenient for data accumulation of positive cases. Therefore, the method for simultaneously detecting 12 alkaline drugs in hair has excellent selectivity, linear correlation, precision and accuracy, and the LOD and the LOQ are low enough, so that technical support is successfully provided for conjecturing drug sources and correctly distinguishing illegal abuse and clinical medication, and considerable market potential is achieved.
Description
Technical Field
The invention belongs to the technical field of drug detection, and particularly relates to a method for simultaneously detecting 12 alkaline drugs in hair.
Background
As is well known, alkaline drugs represented by morphine, codeine, methamphetamine and amphetamine are widely diffused and most harmful in China.
Compared with other biological detection materials such as blood, urine and the like, the hair detection material has the advantages of easiness in obtaining and storing, stability of a target object, long detection time limit and the like, and particularly, the hair detection material can reflect the drug use condition for a long time (several months or years), so that the hair detection material plays an important role in forensic science virus intake identification and becomes evidence for identifying drug addiction in China at present.
In addition, in judicial identification, the detection of drug components in human hair samples is increased to be scientific evidence for identifying drug addicts; however, there is no method for simultaneously detecting multiple drugs, and it is difficult to meet the urgent need of detecting drugs in hair.
However, the methods in the prior art can only aim at one type of drugs, different drug detection requirements require different sample pretreatment operations, and pretreatment is complicated and time-consuming, so that the requirements of real identification can obviously not be met. At present, the types of drugs abused in China are increasingly diversified, and nearly 20% of drug addicts are often mixed with various drugs, such as methamphetamine, ketamine, cocaine and the like. In this case, if analysis is performed only by a method for a single drug, detection omission is likely to occur, and therefore, in practice, a method for screening and analyzing a plurality of drugs and metabolites thereof in hair is urgently required.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides a method for simply, quickly and efficiently detecting 12 alkaline drugs in hair simultaneously, thereby popularizing the analysis technology of the drugs in the hair, standardizing the analysis method of legal medical toxicants in a laboratory and providing technical support for testification, detection and examination of virus taking identification.
Therefore, the invention provides the following technical scheme:
a method for simultaneously detecting 12 alkaline drugs in hair, comprising the steps of:
(1) pretreating a sample;
(2) LC-MS/MS analysis;
wherein, the sample pretreatment in the step (1) comprises the following steps:
washing the hair sample with distilled water and acetone at room temperature, air drying, cutting, and placing in an EP tube; then, adding ceramic grinding beads and an internal standard solution into the EP tube, and carrying out freeze grinding; then, centrifuging, taking the supernatant, transferring the supernatant into a sample injection bottle, and injecting the sample into an LC-MS/MS system;
wherein, the 12 alkaline drugs are: monoacetylmorphine, morphine, codeine, methamphetamine, amphetamine, 3, 4-methylenedioxymethamphetamine (MDMA), 3, 4-Methylenedioxyamphetamine (MDA), 3, 4-methylenedioxy-N-ethyl-amphetamine (MDE), ketamine, norketamine, cocaine, meceutran.
Preferably, in the above method, the internal standard solution is a methanolic solution of methoxamine.
Further preferably, in the above method, the concentration of the internal standard solution is 1 ng/mL.
Preferably, in the above method, the temperature of the freeze-grinding is 10 ℃ or less.
Preferably, in the method, the duration of the freeze grinding is 2-8 min.
Preferably, in the method, the rotation speed of the centrifugation is 10000-13000 rpm.
Further preferably, in the method, the duration of the centrifugation is 2-4 min.
Further preferably, in the method, the sample pretreatment comprises the following specific operations: washing the hair sample with distilled water and acetone sequentially at room temperature for 2 times, air drying, cutting to pieces of about 1mm, and placing 20mg of the pieces in an EP tube; then, 0.3g of ceramic grinding beads and 1mL of an internal standard solution (1 ng/mL of methoxamine) were added to the EP tube, and the mixture was subjected to freeze grinding (10 ℃ C. or lower) for 5 min; then, the mixture was centrifuged at 12000rpm for 3min, and the supernatant was transferred to a sample bottle and introduced into an LC-MS/MS system.
Preferably, in the above method, in the LC-MS/MS analysis of step (2), the chromatographic conditions comprise:
the chromatographic column is an Allure PFP Propyl liquid chromatographic column (2.1mm × 100mm × 5 μm); the mobile phase A is acetonitrile, the mobile phase B is 20mmol/L of amine acetate and 0.1% formic acid buffer solution, and gradient elution is carried out; flow rate: 350 mu L/min; sample introduction amount: 5 mu L of the solution; sample injector temperature: room temperature; column temperature: and (4) room temperature.
Further preferably, in the above method, the conditions of the gradient elution are: 0-4 min: 80% mobile phase a, 20% mobile phase B; 4-4.1 min: 95% mobile phase a, 5% mobile phase B; 4.1-9 min: 80% mobile phase a, 20% mobile phase B; 9min to last: 80% mobile phase a, 20% mobile phase B.
Preferably, in the above method, in the LC-MS/MS analysis of step (2), the mass spectrometry conditions include: electrospray ionization-positive ion mode (ESI +); collision gas (CAD): 7 psi; air curtain gas (CUR): 25 psi; ion source voltage (IS): 5500V; atomizing gas (GS 1): 35 psi; assist gas (GS 2): 35 psi; ion source Temperature (TEM): 500 ℃; the scanning mode is as follows: multiple Reaction Monitoring (MRM).
Compared with the prior art, the technical scheme provided by the invention has the following technical advantages: the method for simultaneously analyzing 12 psychotropic drugs in hair is simple, convenient and rapid, and has wide analysis range. According to the analysis rule of hair identification of abuse drugs issued by the International Hair analysis Association (SOHT), the cutoff values of opioid and phenylpropylamine drugs are 0.2ng/mg, the cutoff values of cocaine and benzoylekonin are 0.5ng/mg and 0.05ng/mg respectively, and the LOD of the method is 0.05ng/mg (20mg hair dose), which is sufficient for meeting the common cases of identification of abuse drugs in hair. The new method is applied to PT sample test of SOHT, and the feasibility, the qualitative and quantitative accuracy are proved. Compared with the existing method, the method omits the liquid-liquid extraction process in the sample pretreatment, the whole process can be completed within 1h, and the time is not increased; in contrast, when multiple samples are analyzed, the advantage of high throughput is more pronounced. The technical scheme provided by the invention can realize external pollution detection and instant quantitative analysis of actual cases, and is convenient for data accumulation of positive cases.
In conclusion, the method for simultaneously detecting 12 alkaline drugs in hair has excellent selectivity, linear correlation, precision and accuracy, and the LOD and the LOQ are low enough, so that technical support is provided for predicting drug sources and correctly distinguishing illegal abuse and clinical medication successfully, and therefore, the method has considerable market potential.
Drawings
FIG. 1 is an LC-MS/MS spectrum of a hair sample from example 1;
FIG. 2 is an LC-MS/MS spectrum of a hair sample from example 2;
FIG. 3 is an LC-MS/MS spectrum of a hair sample from example 3;
FIG. 4 is a total ion current chromatogram of 12 kinds of alkaline drugs;
FIG. 5 is an LC-MS/MS spectrum of monoacetylmorphine;
figure 6 is an LC-MS/MS profile of morphine;
FIG. 7 is an LC-MS/MS spectrum of codeine;
FIG. 8 is an LC-MS/MS spectrum of methamphetamine;
FIG. 9 is an LC-MS/MS spectrum of amphetamine;
FIG. 10 is an LC-MS/MS profile of MDMA;
FIG. 11 is an LC-MS/MS spectrum of MDA;
FIG. 12 is an LC-MS/MS spectrum of MDE;
FIG. 13 is an LC-MS/MS spectrum of ketamine;
FIG. 14 is an LC-MS/MS spectrum of norketamine;
FIG. 15 is an LC-MS/MS profile of cocaine;
FIG. 16 is an LC-MS/MS spectrum of benzoylekonin;
FIG. 17 is an LC-MS/MS spectrum of the internal standard.
Detailed Description
The present invention will be further described with reference to specific embodiments, but the present invention is not limited to the following embodiments.
A method for simultaneously detecting 12 alkaline drugs in hair comprises the following steps:
(1) pretreating a sample;
(2) LC-MS/MS analysis;
wherein, the sample pretreatment in the step (1) comprises the following steps:
washing the hair sample with distilled water and acetone at room temperature, air drying, cutting, and placing in an EP tube; then, adding ceramic grinding beads and an internal standard solution into the EP tube, and carrying out freeze grinding; then, centrifuging, taking the supernatant, transferring the supernatant into a sample injection bottle, and injecting the sample into an LC-MS/MS system;
wherein, the 12 alkaline drugs are: monoacetylmorphine, morphine, codeine, methamphetamine, amphetamine, 3, 4-methylenedioxymethamphetamine (MDMA), 3, 4-Methylenedioxyamphetamine (MDA), 3, 4-methylenedioxy-N-ethyl-amphetamine (MDE), ketamine, norketamine, cocaine, meceutran.
In a preferred embodiment, the internal standard solution is a methanolic solution of methoxamine.
In a further preferred embodiment, the concentration of the internal standard solution is 1 ng/mL.
In a preferred embodiment, the temperature of the freeze-grinding is 10 ℃ or less.
In a preferred embodiment, the duration of the freeze-grinding is 2-8 min.
In a preferred embodiment, the rotating speed of the centrifugation is 10000-13000 rpm.
In a further preferred embodiment, the duration of the centrifugation is 2 to 4 min.
In a preferred embodiment, in the LC-MS/MS analysis described in step (2), the chromatographic conditions comprise:
the chromatographic column is an Allure PFP Propyl liquid chromatographic column (2.1mm × 100mm × 5 μm); the mobile phase A is acetonitrile, the mobile phase B is 20mmol/L of amine acetate and 0.1% formic acid buffer solution, and gradient elution is carried out; flow rate: 350 mu L/min; sample introduction amount: 5 mu L of the solution; sample injector temperature: room temperature; column temperature: and (4) room temperature.
In a further preferred embodiment, the conditions of the gradient elution are as shown in table 1 below:
TABLE 1 conditions of gradient elution
| Time (min) | A(v％) | B(v％) |
| Initial | 80 | 20 |
| 1.00 | 80 | 20 |
| 4 | 95 | 5 |
| 4.10 | 80 | 20 |
| 9.00 | 80 | 20 |
In a preferred embodiment, in the LC-MS/MS analysis of step (2), the mass spectrometry conditions comprise: electrospray ionization-positive ion mode (ESI +); collision gas (CAD): 7 psi; air curtain gas (CUR): 25 psi; ion source voltage (IS): 5500V; atomizing gas (GS 1): 35 psi; assist gas (GS 2): 35 psi; ion source Temperature (TEM): 500 ℃; the scanning mode is as follows: multiple Reaction Monitoring (MRM).
Among these, the detected ions and other related parameters are shown in table 2 below:
TABLE 2 MS/MS conditions and Retention times for the Compounds
Note: first parent/daughter ion pair is a quantitative ion pair.
In addition, the inventor also carries out a series of tests to verify the method, which mainly comprises verifying the selectivity, the linear correlation, the quantitative limit, the precision, the accuracy and the like of the method; as follows respectively:
the blank hair is subjected to sample pretreatment according to the method of the invention and then subjected to LC-MS/MS analysis, and endogenous substances in the blank hair do not interfere with the measurement of 12 alkaline drugs, metabolites thereof and internal standards, so the method of the invention has good selectivity.
The 12 alkaline drugs and their metabolites showed good linearity in their respective linear ranges, as shown in Table 3 below, LOD was 0.05ng/mg, and LOQ was 0.1 ng/mg.
TABLE 3 Linear equation and Linear Range
In addition, the accuracy of the concentrations of the 12 alkaline drugs and metabolites thereof at the low, medium and high concentrations is within 100 +/-20%, and the precision RSD is within +/-20%, which is shown in the following table 4.
TABLE 4 precision and accuracy of measurement of 12 alkaline drugs and their metabolites in hair
Example 1
If a person sends out the hair to be detected, the suspect claims that the person never hit the drugs. The hair to be inspected is analyzed by adopting the method of the invention, not only methamphetamine and the metabolite amphetamine thereof are detected in the 0-3cm section of the scalp, but also MDMA and the metabolite MDA thereof are detected, and the specific detection result is shown in figure 1.
Example 2
When a certain party sends out the hair to be detected, the suspects acknowledge the smooth 'ice'; the hair to be inspected is analyzed by adopting the method disclosed by the invention, so that not only methamphetamine and a metabolite thereof, namely amphetamine, are detected, but also ketamine and a metabolite thereof, namely norketamine, are detected; therefore, by applying the method of the present invention, a plurality of alkaline drugs can be simultaneously screened to reduce the risk of missing detection, and the detection result of this embodiment is shown in fig. 2.
Example 3
When a certain party sends out the hair to be detected, the suspects acknowledge the smooth 'ice'; the hair to be inspected is analyzed by adopting the method disclosed by the invention, not only MDMA and MDA metabolite thereof are detected, but also ketamine and methyl ketamine metabolite thereof, cocaine and benzoyl ekonin metabolite thereof are detected; therefore, by applying the method of the present invention, a plurality of alkaline drugs can be simultaneously screened to reduce the risk of missing detection, and the detection result of this embodiment is shown in fig. 3.
The embodiments of the present invention have been described in detail, but the embodiments are only examples, and the present invention is not limited to the above-described embodiments. Any equivalent modifications and substitutions to those skilled in the art are also within the scope of the present invention. Therefore, equivalent changes and modifications made without departing from the spirit and scope of the present invention should be covered by the present invention.
Claims (7)
1. A method for simultaneously detecting 12 alkaline drugs in hair is characterized by comprising the following steps:
1) pretreating a sample;
2) LC-MS/MS analysis;
wherein, the sample pretreatment in the step 1) comprises the following steps:
washing the hair sample with distilled water and acetone at room temperature, air drying, cutting, and placing in an EP tube; then, adding ceramic grinding beads and an internal standard solution into the EP tube, and carrying out freeze grinding; then, centrifuging, taking the supernatant, transferring the supernatant into a sample injection bottle, and injecting the sample into an LC-MS/MS system;
wherein, the 12 alkaline drugs are: monoacetylmorphine, morphine, codeine, methamphetamine, amphetamine, 3, 4-methylenedioxymethamphetamine, 3, 4-methylenedioxyamphetamine, 3, 4-methylenedioxy-N-ethyl-amphetamine, ketamine, norketamine, cocaine, benzoylekanine;
wherein the internal standard solution is a methanol solution of methoxyphenamine;
in the LC-MS/MS analysis in the step 2), the chromatographic conditions comprise:
the chromatographic column is an Allure PFP Propyl liquid chromatographic column, 2.1mm multiplied by 100mm multiplied by 5 mu m; the mobile phase A is acetonitrile, the mobile phase B is 20mmol/L of amine acetate and 0.1% formic acid buffer solution, and gradient elution is carried out; flow rate: 350 mu L/min; sample introduction amount: 5 mu L of the solution; sample injector temperature: room temperature; column temperature: room temperature;
the conditions of the gradient elution are as follows: 0-4 min: 80% mobile phase a, 20% mobile phase B; 4-4.1 min: 95% mobile phase a, 5% mobile phase B; 4.1-9 min: 80% mobile phase a, 20% mobile phase B; 9min to the end: 80% mobile phase a, 20% mobile phase B.
2. The method for simultaneously detecting 12 alkaline drugs in hair according to claim 1, wherein the concentration of the internal standard solution is 1 ng/mL.
3. The method for simultaneously detecting 12 alkaline drugs in hair according to claim 1, wherein the temperature of the freeze-grinding is 10 ℃ or lower.
4. The method for simultaneously detecting 12 alkaline drugs in hair according to claim 1, wherein the duration of the freeze-grinding is 2-8 min.
5. The method for simultaneously detecting 12 alkaline drugs in hair according to claim 1, wherein the rotation speed of the centrifugation is 10000-13000 rpm.
6. The method for simultaneously detecting 12 alkaline drugs in hair according to claim 5, wherein the duration of centrifugation is 2-4 min.
7. The method for simultaneously detecting 12 alkaline drugs in hair according to claim 1, wherein in the LC-MS/MS analysis in step 2), the mass spectrometry conditions comprise:
electrospray ionization-positive ion mode, ESI +; collision gas, CAD: 7 psi; air curtain gas, CUR: 25 psi; ion source voltage, IS: 5500V; atomizing gas, GS 1: 35 psi; auxiliary gas, GS 2: 35 psi; ion source temperature, TEM: 500 ℃; the scanning mode is as follows: multiple reaction monitoring, MRM.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810375411.7A CN108627595B (en) | 2018-04-24 | 2018-04-24 | Method for simultaneously detecting 12 alkaline drugs in hair |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810375411.7A CN108627595B (en) | 2018-04-24 | 2018-04-24 | Method for simultaneously detecting 12 alkaline drugs in hair |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108627595A CN108627595A (en) | 2018-10-09 |
| CN108627595B true CN108627595B (en) | 2021-04-09 |
Family
ID=63694520
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810375411.7A Active CN108627595B (en) | 2018-04-24 | 2018-04-24 | Method for simultaneously detecting 12 alkaline drugs in hair |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108627595B (en) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109613126B (en) * | 2018-11-01 | 2020-11-03 | 中国环境科学研究院 | Method for determining illegal drugs and metabolites thereof in fish body |
| CN109828039A (en) * | 2018-12-20 | 2019-05-31 | 广州市圣鑫生物科技有限公司 | The kit of common illicit drugs inspection, pre-treating method and detection method in hair |
| CN110146462A (en) * | 2019-06-10 | 2019-08-20 | 程军亮 | The analysis method of heroin identical source |
| CN110987548A (en) * | 2019-10-11 | 2020-04-10 | 广州万孚生物技术股份有限公司 | Method for detecting hair poison, pretreatment reagent and kit |
| CN110988205B (en) * | 2019-11-26 | 2021-08-10 | 厦门历思科技服务有限公司 | Novel method for detecting substance intake and intake frequency of human body |
| CN111426768A (en) * | 2020-04-21 | 2020-07-17 | 司法鉴定科学研究院 | Analysis method for simultaneously determining 11 kinds of drugs and metabolites thereof in domestic sewage |
| CN111879843B (en) * | 2020-07-15 | 2023-06-06 | 宁波华仪宁创智能科技有限公司 | Method for detecting drugs in hair |
| CN111855854A (en) * | 2020-07-29 | 2020-10-30 | 公安部物证鉴定中心 | Hair detection method for screening drug addicts |
| CN112213412A (en) * | 2020-09-01 | 2021-01-12 | 四川警察学院 | Method for detecting drug and metabolite thereof in hair |
| CN114264732A (en) * | 2020-09-16 | 2022-04-01 | 广州金域司法鉴定技术有限公司 | Method for detecting drugs in hair |
| CN113311084B (en) * | 2021-05-24 | 2023-06-16 | 公安部鉴定中心 | Hair poison quality control product for criminal investigation field and rapid preparation method thereof |
| CN113325103A (en) * | 2021-05-28 | 2021-08-31 | 司法鉴定科学研究院 | Analysis method for simultaneously measuring gelsemium, gelsemine and gelsemine in hair |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101398414A (en) * | 2008-10-15 | 2009-04-01 | 上海市公安局刑事侦查总队 | Method for qualitatively screening 242 kinds of compounds by liquid phase chromatography-mass spectra at the same times |
| CN103207242A (en) * | 2012-01-11 | 2013-07-17 | 司法部司法鉴定科学技术研究所 | Method for rapidly identifying monoacetylmorphine and morphine in hair |
| CN103575831A (en) * | 2013-09-24 | 2014-02-12 | 广州正孚检测技术有限公司 | Extraction and detection method for ketamine, norketamine and amphetamin-type substances in hairs |
| CN107449644A (en) * | 2017-07-10 | 2017-12-08 | 张红丽 | A kind of method of amphetamine and drugs such as morphine in rapid field examination hair |
| CN107917970A (en) * | 2017-10-11 | 2018-04-17 | 广东南天司法鉴定所 | The method of examination psychotropic agent from hair |
-
2018
- 2018-04-24 CN CN201810375411.7A patent/CN108627595B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101398414A (en) * | 2008-10-15 | 2009-04-01 | 上海市公安局刑事侦查总队 | Method for qualitatively screening 242 kinds of compounds by liquid phase chromatography-mass spectra at the same times |
| CN103207242A (en) * | 2012-01-11 | 2013-07-17 | 司法部司法鉴定科学技术研究所 | Method for rapidly identifying monoacetylmorphine and morphine in hair |
| CN103575831A (en) * | 2013-09-24 | 2014-02-12 | 广州正孚检测技术有限公司 | Extraction and detection method for ketamine, norketamine and amphetamin-type substances in hairs |
| CN107449644A (en) * | 2017-07-10 | 2017-12-08 | 张红丽 | A kind of method of amphetamine and drugs such as morphine in rapid field examination hair |
| CN107917970A (en) * | 2017-10-11 | 2018-04-17 | 广东南天司法鉴定所 | The method of examination psychotropic agent from hair |
Non-Patent Citations (4)
| Title |
|---|
| HPLC-LTQ-Orbitrap-MS在毒、药物筛选分析中的应用研究;李晓雯;《中国优秀硕士学位论文全文数据库 医药卫生科技辑》;20130315;第E079-74页 * |
| Screening and quantitation of multiclass drugs of abuse and pharmaceuticals in hair by fast liquid chromatography electrospray time-of-flight mass spectrometry;Juan C.Domínguez-Romero 等;《Journal of Chromatography B》;20110715;第879卷(第22期);第2034-2042页 * |
| Simultaneous determination of morphine, codeine, 6-acetylmorphine, cocaine and benzoylecgonine in hair by liquid chromatography/electrospray ionization tandem mass spectrometry;Huang, DK 等;《RAPID COMMUNICATIONS IN MASS SPECTROMETRY》;20090224;第23卷(第7期);第957-962页 * |
| 吸毒者头发中海洛因、6-单乙酰吗啡、吗啡、可待因和乙酰可待因的分析及评价;向平 等;《中国司法鉴定》;20130131(第1期);第19-29页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108627595A (en) | 2018-10-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108627595B (en) | Method for simultaneously detecting 12 alkaline drugs in hair | |
| Fabresse et al. | Development of a sensitive untargeted liquid chromatography–high resolution mass spectrometry screening devoted to hair analysis through a shared MS2 spectra database: A step toward early detection of new psychoactive substances | |
| O’Donnell et al. | Pharmaceutical applications of ion mobility spectrometry | |
| Kyle | Toxicology: gcMS | |
| Lynch | Toxicology: liquid chromatography mass spectrometry | |
| CN111487336B (en) | Method for analyzing 37 fentanyl novel psychoactive substances in hair | |
| CN105388245A (en) | Screening method suitable for 46 kinds of medicine in aquatic products | |
| Hong et al. | Determination of synthetic cathinones in urine using gas chromatography–mass spectrometry techniques | |
| CN112213412A (en) | Method for detecting drug and metabolite thereof in hair | |
| Ellison et al. | Comprehensive analysis of drugs of abuse in urine using disposable pipette extraction | |
| Teng et al. | Screening of drugs of abuse and toxic compounds in human whole blood using online solid‐phase extraction and high‐performance liquid chromatography with time‐of‐flight mass spectrometry | |
| Pablo et al. | Automated analysis of dried urine spot (DUS) samples for toxicology screening | |
| CN112326819B (en) | Analysis method for simultaneously determining etizolam, flunitrazepam and 7 amino flunitrazepam in hair | |
| McMillin et al. | Demystifying analytical approaches for urine drug testing to evaluate medication adherence in chronic pain management | |
| Strano‐Rossi et al. | Rapid screening of drugs of abuse and their metabolites by gas chromatography/mass spectrometry: application to urinalysis | |
| Ahrens et al. | Detection of stimulants and narcotics by liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry for sports doping control | |
| US6808933B1 (en) | Methods of enhancing confidence in assays for analytes | |
| Neill et al. | Automated screening procedure using gas chromatography—mass spectrometry for identification of drugs after their extraction from biological samples | |
| CN108845063B (en) | Detection reagent combination and detection method of aquatic product additive | |
| Marin et al. | Comparison of drug detection by three quadrupole time-of-flight mass spectrometry platforms | |
| Wickremsinhe et al. | Validating regulatory‐compliant wide dynamic range bioanalytical assays using chip‐based nanoelectrospray tandem mass spectrometry | |
| CN101813675A (en) | LC-MS/MS analysis method for detecting morphinane alkaloid in whole blood and urine | |
| Usui et al. | Direct detection of the psychoactive substance MT‐45 in human tissue samples by probe electrospray ionization‐tandem mass spectrometry | |
| CN101169417A (en) | Reagent kit and method for judging normal person and liver cancer using magnetic bead supported matrix | |
| Cristoni et al. | Surface‐activated chemical ionization ion trap mass spectrometry in the analysis of drugs in dilute urine samples. Part II: analysis of morphine and other street drugs |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |