CN108610097A - A kind of amino acid liquid fertilizer and its application in corn planting - Google Patents

A kind of amino acid liquid fertilizer and its application in corn planting Download PDF

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CN108610097A
CN108610097A CN201810955814.9A CN201810955814A CN108610097A CN 108610097 A CN108610097 A CN 108610097A CN 201810955814 A CN201810955814 A CN 201810955814A CN 108610097 A CN108610097 A CN 108610097A
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amino acid
corn
nitrogen
arginine
increases
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CN108610097B (en
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李学贤
罗惠兰
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China Agricultural University
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China Agricultural University
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C11/00Other nitrogenous fertilisers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20Cereals
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • C05G5/23Solutions

Abstract

The present invention relates to a kind of amino acid liquid fertilizer and its applications in corn planting.Specifically, the present invention provides a kind of amino acid liquid fertilizer, the amino acid liquid fertilizer includes the water of amino acid and surplus, and the amino acid is arginine or glutamine.The present invention also provides a kind of corn planting method, the method includes:The amino acid liquid fertilizer is sprayed to corn.It is the application of arginine and/or glutamine in improving corn grouting effect the present invention also provides amino acid.The amino acid liquid fertilizer formula of the present invention is simple, it is easy to use, fertilizer efficiency is notable, the grouting effect of corn can be improved comprehensively for maize planting, corncob phenotype (including grain plumpness improves, cob length increases, kernal number increases and bald sharp phenomenon mitigates) is especially significantly improved, mass of 1000 kernel, grain number per spike, Ear weight, seed carotenoid concentration etc. are improved.

Description

A kind of amino acid liquid fertilizer and its application in corn planting
Technical field
The present invention relates to corn planting technical fields, and in particular to a kind of amino acid liquid is fertile and its in corn planting Using.
Background technology
During agricultural development, fertilizer plays a crucial role.Lead Chinese agriculture field the nineties in last century " outstanding problem and suggestion that China's chemical fertilizer faces " report that the brainstrust of developing direction proposes promotes China's centering, micro (Li Yong, Yu Guangwu, Li Xiaobing wait middle and trace element Fertilizer developments for the attention of element fertilizer, new-type fertilizer and environmental protection agricultural Necessity and its Analysis on Prospect [J] chemical fertilizer industries, 2013,40 (4):1-3.).Hereafter, occur largely being directed to multi-component composite fertilizer The research of effect.
In order to which the effect for allowing multiple element to be administered simultaneously is more than the effect applied respectively, middle and trace element must be just solved Between antagonism problem and each element influenced to reduce the problem of plant absorption utilizes by soil pH.Since amino acid is not by soil Earth pH and other ions influence, therefore are often used as the chelating agent (life of Shao Jianhua, Ma Xiaoxin Amino Acid Chelated Mineral Fertilizers of trace element Production and the Guangdong application study progress [J] trace element science, 2000,7 (9):40-42;Wang Chen, Liu Chao, the spray of the blade faces the such as horse Jing Apply influence [J] Chinese soil and fertilizer of the amino acid-zinc complexes to rice yield traits and zinc-iron alloy solution, 2017 (4):118- 123.)。
However, the amino acid being currently known kind of (Wagner I, Musso H.New Naturally up to more than 500 Occurring Amino Acids [J] .Angewandte Chemie International Edition, 2010,22 (11): 816-828.), the amino acid and used in fertilizer at present is mostly compound amino acid (including a variety of amino acid) rather than single ammonia Base acid.Early in 1997, just studies have found that kilnitamin fertilizer efficiency be considered higher than etc. nitrogen quantity single amino acid (Xu Yu Orchid, Liu Qingcheng Fertilizer Efficiency of Amino Acids research [J] amino acid and living resources, 1997 (2):1-6.).
Corn (Zea mays L.) has a decisive role in terms of world food and energy consumption.As corn Important growing stage in growth and development process, grouting directly affect corn kernel dry-matter accumulation amount.In addition, bald tip of corn is existing As the generally existing in Production of Large Fields, bald point phenomenon not only causes nutrient consumption, but also reduces seed quantity, the production to corn Amount and quality cause extreme influence.Bald tip of corn after female fringe abortion of spikelets or little Hua inseminations mainly by failing normally to be in the milk (woods, Rong Tingzhao, II, maize ear different parts little Hua of research, the seed of the green bald tip of corn of topaz are prayed by Tang caused by abortion seed Differentiation and development period [J] Sichuan Agricultural University's journal, 1999,17 (2):162-166.).Some scholars by corn variety, Macro-scales research bald tip of corn Producing reason (Zhang Wenzhong, Song Dianzhen, the chestnut such as soil fertility condition, planting density and climatic factor Red-face role waits Reason Analysis on Barren Ear Tip of Maize [J] Exploitation of Agriculture in Heilongjiang science, 2007,2007 (6):20-22.), some research attempts Establishing hormonal readiness, (woods, relationship [J] nuclear agricultural science reports of the clear bald tip of corn of Rong Ting and endogenous hormones, 2007,21 (4) are prayed by Tang: 366-368.), plant vasular beam (grind by the comparison of He Qiping, Dong Shuting, high Rong Qi different types corn variety Ear vascular bundles Study carefully [J] Acta Agronomica Sinicas, 2007,33 (7):1187-1196.) being associated between bald tip of corn, some scholars from heredity and molecule On the basis of dissect bald tip of corn phenomenon (ability source, Li Kai, Jiang Tao wait the Genetic Model Analysis and SSR marker of bald tip of corn characters [J] Jilin Auto Industries, 2015,37 (1):6-13.).The research of forefathers is more to the preliminary of bald tip of corn reason It probes into, fails to the practical operable method for solving the problems, such as this propose with universality, specific.
Invention content
The problems such as in order to solve such as bald tip of corn phenomenon, to improve the yield of corn.The present invention is carried in first aspect A kind of amino acid liquid fertilizer is supplied, wherein the amino acid liquid fertilizer includes the water of amino acid and surplus, and the amino acid is essence Propylhomoserin or glutamine.
Second aspect of the present invention provides a kind of corn planting method, wherein the method includes:It is sprayed to corn and includes The liquid fertilizer of the amino acid of 0.5mM to 2.5mM, the amino acid are arginine and/or glutamine.
Third aspect present invention provides application of the amino acid in improving corn grouting effect, wherein the amino acid For arginine and/or glutamine.
The present invention has the following technical effect that:(1) corncob phenotype improves, including grain plumpness improves, cob length Increase, kernal number increases and bald sharp phenomenon mitigates;(2) mass of 1000 kernel increases, and increment is even as high as 22% or more in the kind having; (3) grain number per spike increases, and increment is even as high as 54% or more in the kind having;(4) Ear weight increases, and increment is in the kind having Even as high as 88% or more;(5) seed carotenoid concentration increases, and increment is even as high as 13% or more in the kind having.And And amino acid liquid fertilizer formula of the invention is simple, easy to use, fertilizer efficiency is notable.The implantation methods or amino acid of the present invention Liquid fertilizer can improve the planting effect of corn comprehensively, it is particularly possible to grouting effect during corn planting for improving corn Fruit.
Description of the drawings
Fig. 1 is normally to handle post-grouting in different aminoacids state nitrogen for corn hybrid seed Zheng Dan 958 (ZD958) under the conditions of nitrogen The phenotypic analysis in period.
Fig. 2 is less than normally for corn hybrid seed Zheng Dan 958 (ZD958) under the conditions of the nitrogen level of nitrogen in different aminoacids state Nitrogen handles the phenotypic analysis in post-grouting period.
Fig. 3 is that normally for corn hybrid seed under the conditions of nitrogen, first 335 (XY335) of jade handle post-grouting in different aminoacids state nitrogen The phenotypic analysis in period.
Fig. 4 is less than normally for first 335 (XY335) of jade of corn hybrid seed under the conditions of the nitrogen level of nitrogen in different aminoacids state Nitrogen handles the phenotypic analysis in post-grouting period.
Fig. 5 is normally for good beautiful 99 (LY99) of corn hybrid seed under the conditions of nitrogen when different aminoacids state nitrogen handles post-grouting The phenotypic analysis of phase.
Fig. 6 is less than normally for good beautiful 99 (LY99) of corn hybrid seed under the conditions of the nitrogen level of nitrogen in different aminoacids state nitrogen Handle the phenotypic analysis in post-grouting period.
Fig. 7 is normally to step on 105 (DH105) of sea for corn hybrid seed under the conditions of nitrogen to handle post-grouting in different aminoacids state nitrogen The phenotypic analysis in period.
Fig. 8 is less than normally steps on 105 (DH105) of sea in different aminoacids state for corn hybrid seed under the conditions of the nitrogen level of nitrogen Nitrogen handles the phenotypic analysis in post-grouting period.
Fig. 9 is the cob length for normally handling post-grouting period in different aminoacids state nitrogen for corn hybrid seed under the conditions of nitrogen Analysis.
Figure 10 is less than normally handles post-grouting for corn hybrid seed under the conditions of the nitrogen level of nitrogen in different aminoacids state nitrogen The cob length analysis in period.
Figure 11 is the mass of 1000 kernel for normally handling post-grouting period in different aminoacids state nitrogen for corn hybrid seed under the conditions of nitrogen Analysis.
Figure 12 is less than normally handles post-grouting for corn hybrid seed under the conditions of the nitrogen level of nitrogen in different aminoacids state nitrogen The mass of 1000 kernel in period is analyzed.
Figure 13 is the grain number per spike for normally handling post-grouting period in different aminoacids state nitrogen for corn hybrid seed under the conditions of nitrogen Analysis.
Figure 14 is less than normally handles post-grouting for corn hybrid seed under the conditions of the nitrogen level of nitrogen in different aminoacids state nitrogen The grain number per spike in period is analyzed.
Figure 15 is the Ear weight for normally handling post-grouting period in different aminoacids state nitrogen for corn hybrid seed under the conditions of nitrogen Analysis.
Figure 16 is less than normally handles post-grouting for corn hybrid seed under the conditions of the nitrogen level of nitrogen in different aminoacids state nitrogen The Ear weight in period is analyzed.
Figure 17 is the seed class for normally handling post-grouting period in different aminoacids state nitrogen for corn hybrid seed under the conditions of nitrogen Concentration of carotene is analyzed.
Figure 18 is less than normally handles post-grouting for corn hybrid seed under the conditions of the nitrogen level of nitrogen in different aminoacids state nitrogen The seed carotenoid concentration in period is analyzed.
Figure 19 is the section nitrogen potentiality for showing arginine or glutamine in different cultivars.
Specific implementation mode
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be by way of example to this hair Bright specific implementation mode is more clearly and completely described, it is clear that described specific implementation mode is the one of the present invention Section Example, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not doing The every other embodiment obtained under the premise of going out creative work, shall fall within the protection scope of the present invention.
First aspect present invention provides a kind of amino acid liquid fertilizer, wherein the amino acid liquid fertilizer includes amino acid With the water of surplus, the amino acid is arginine or glutamine.Preferably, a concentration of 0.5mM of the amino acid is extremely 2.5mM, such as 1.25mM.Certainly, as long as leading to unacceptable adverse effect, other solvents can be used to replace water.
The inventors discovered that although compound amino acid fertilizer is at low cost compared with single amino acid, compound amino acid Different aminoacids in fertilizer may have mutual antagonism to the physiological effect of specified plant.Glutamine (glutamine, Gln it) can be synthesized by the ammonium nitrogen being absorbed into plant, and participate in process (the Forde B that glutamic acid forms acidic amino acid G, Lea P J.Glutamate in plants:Metabolism, regulation, and signalling. [J] .Journal of Experimental Botany, 2007,58 (9):2339-2358.).L-arginine can be store in plant Nitrogen is hidden for recycling and the precursor substance of some signal of interest molecules, participates in the physiology mistake such as growth and development of plants, degeneration-resistant Journey (physiological function [J] molecules plant (English edition) of Yang Hongqiang, high China's monarch's plants arginine and its metabolite, 2007,33 (1):1-8.).The present inventor carries out foliage-spray by the discovery that studies for a long period of time, using arginine or the combination of glutamine single The grouting effect that corn can be significantly improved, so as to considerably improve the yield of corn.
The present invention provides a kind of corn planting method in second aspect, wherein the method includes:Packet is sprayed to corn The amino acid liquid fertilizer of amino acid containing 0.5mM to 2.5mM, the amino acid are arginine and/or glutamine.Aminoacids solution The concentration of state fertilizer can be 0.5mM between 2.5mM arbitrary value or arbitrary subrange, for example, 0.50,0.75,1.00, 1.25,1.5,1.75,2.00,2.25 or 2.50mM, most preferably 1.25mM.The amino acid can be arginine, glutamy Amine, more preferably glutamine.
In some preferred embodiments, described to spray complete selected from phase, the 8th leaf is fully deployed by the 6th leaf Expansion phase, the 12nd leaf are fully deployed the phase and one or more period of the group for the phase composition that spins carries out.It is preferred that it is described according to It is secondary to carry out foliage-spray in aforementioned four period.
In some preferred embodiments, the amount sprayed is 250g/hm2To 350g/hm2Arginine or 200g/ hm2To 300g/hm2Glutamine.
The present invention is not particularly limited the supply of nitrogen in planting process, is applied according to normal applying Namount. In some preferred embodiments, the method further includes providing 0 to 200kg/hm2Nitrogenous fertilizer, for example, 50,100,150 or 200kg/hm2.It is further preferred that with 200kg/hm2Normal applying Namount for nitrogen, can so ensure arginine or glutamine Promoting the effect that cob length increases can more fully play.
The present invention is not particularly limited corn variety.For example, the corn is hybrid maize.It is preferred real at some It applies in mode, the hybrid maize is selected from by the group that first jade 335, Zheng Dan 958, good beautiful 99 and Deng Hai 605 are formed, most preferably First jade 335 and good beautiful 99, the effect that the amino acid improves it is especially pronounced.These corn varieties are all known kinds, can With commercially available from such as Beijing Si Nongzhong industry Co., Ltd,
The present invention provides application of the amino acid in improving corn grouting effect in the third aspect, wherein the amino Acid is arginine and/or glutamine.As described above, more preferably glutamine.
In some preferred embodiments, the improvement corn grouting effect shows as one or more following index Improvement:Corncob phenotype, mass of 1000 kernel, Ear weight, grain number per spike, seed carotenoid concentration.In some preferred embodiments In, the corncob phenotype is selected from the group being made of grain plumpness, cob length, kernal number and bald sharp phenomenon.
In some preferred embodiments, the improvement corn grouting effect is shown as:Corncob phenotype improves, packet Include grain plumpness raising, cob length increases, kernal number increases and bald sharp phenomenon mitigates;Mass of 1000 kernel increases, it is preferred that thousand Grain increases by 2.81% to 22.06% again;Grain number per spike increases, it is preferred that grain number per spike increases by 7.79% to 54.61%;Ear weight Increase, it is preferred that Ear weight increases by 11.08% to 88.56%;And/or seed carotenoid concentration increases, it is preferred that Seed carotenoid concentration increases by 1.65% to 13.11%.
Embodiment
Technical scheme of the present invention will be hereafter illustrated in the form of embodiment, but the protection model of the present invention It encloses and is not limited to these embodiments.
1. materials and methods
Place:The village Bei Jingshang experiment station of China Agricultural University.
Corn strain:First jade 335 (XY335), Zheng Dan 958 (ZD958), good beautiful 99 (LY99) and step on 605 (DH605) of sea. These corn varieties are purchased from Beijing Si Nongzhong industry Co., Ltd.
1.1 normally for nitrogen (CK, 200 kg/ha of nitrogen (urea)) and low nitrogen (LN, 0 kg/ha of nitrogen) Under the conditions of be handled as follows:
(1) 1 (T1) is handled:Spray water;
(2) 2 (T2) are handled:Spray the arginine of 1.25mM;
(3) 3 (T3) are handled:Spray the glutamine of 1.25mM.
It sprays and is carried out in following period:(a) period 1 (ST1):The 6th leaf of corn is fully deployed (corn jointing stage);(b) Period 2 (ST2):The 8th leaf of corn is fully deployed;(c) period 3 (ST3):The 12nd leaf of corn is fully deployed (corn typhon The mouth phase);(d) period 4 (ST4):The corn silking phase.Water content in harvest:At the end of corn is in the milk.Biology repeats:4 (i.e. n= 4).Experimental plot area uses RANDOMIZED BLOCK DESIGN.
2. result and analysis
2.1 phenotypic analysis
2.1.1 Zheng Dan 958 (ZD958)
Corn hybrid seed Zheng Dan 958 (ZD958) is analyzed under the conditions of normally for nitrogen (CK) at different aminoacids state nitrogen Manage the phenotype in post-grouting period.As a result, it has been found that arginine (T2) and the processed Zheng Dan 958 of glutamine (T3) difference, are filling The relatively control (T1) of slurry period seed is fuller, in addition, the corncob length of glutamine processing is considerably longer than and compares (such as Fig. 1 It is shown).
(LN) corn hybrid seed Zheng Dan 958 (ZD958) is also analyzed under the conditions of less than normally for the nitrogen level of nitrogen not The phenotype in post-grouting period is handled with amino-acid nitrogen.As a result, it has been found that arginine (T2) and glutamine (T3) process respectively Zheng Dan 958, grouting period cob length relatively control (T1) difference it is smaller (as shown in Figure 2).
2.1.2 335 (XY335) of first jade
First 335 (XY335) of jade of the corn hybrid seed under normally for nitrogen condition (CK) are analyzed at different aminoacids state nitrogen Manage the phenotype in post-grouting period.As a result, it has been found that arginine (T2) and the processed first jade 335 of glutamine (T3) difference, are filling Slurry period kernal number and cob length all relatively compare (T1) more, in addition, glutamine processing alleviates the bald sharp phenomenon of corn (as shown in Figure 3).
It also analyzes less than normally for first 335 (XY335) of jade of corn hybrid seed under the nitrogen level condition (LN) of nitrogen in difference Amino-acid nitrogen handles the phenotype in post-grouting period.As a result, it has been found that arginine (T2) and glutamine (T3) are processed respectively First jade 335, fuller in the relatively control (T1) of grouting period seed, kernal number and cob length all relatively compare more, glutamine Processing also mitigates the bald sharp phenomenon (as shown in Figure 4) of corn.
2.1.3 good beautiful 99 (LY99)
Good beautiful 99 (LY99) of the corn hybrid seed under normally for nitrogen condition (CK) are analyzed in the processing of different aminoacids state nitrogen The phenotype in post-grouting period.As a result, it has been found that arginine (T2) and glutamine (T3) difference processed good beautiful 99, in grouting The relatively control (T1) of phase seed is fuller, and kernal number relatively compares more, and cob length is also longer (as shown in Figure 5).
Also analyze less than normally for the nitrogen level condition (LN) of nitrogen under good beautiful 99 (LY99) of corn hybrid seed in difference Amino-acid nitrogen handles the phenotype in post-grouting period.As a result, it has been found that arginine (T2) and glutamine (T3) are processed respectively Good beautiful 99, fuller in the relatively control (T1) of grouting period seed, all relatively control is more (such as Fig. 6 institutes for kernal number and cob length Show).
2.1.4 105 (DH105) of sea are stepped on
It analyzes the corn hybrid seed under normally for nitrogen condition (CK) and steps on 105 (DH105) of sea at different aminoacids state nitrogen Manage the phenotype in post-grouting period.As a result, it has been found that arginine (T2) and glutamine (T3) are processed respectively to step on sea 105, filling The relatively control (T1) of slurry period cob length is longer (as shown in Figure 7).
Also analyze less than normally for the nitrogen level condition (LN) of nitrogen under corn hybrid seed step on 105 (DH105) of sea not The phenotype in post-grouting period is handled with amino-acid nitrogen.As a result, it has been found that arginine (T2) and glutamine (T3) process respectively Step on sea 105, fuller in the relatively control (T1) of grouting period seed, kernal number and cob length all relatively compare more (such as Fig. 8 institutes Show).
In general, arginine and glutamine can improve grain plumpness and single tassel seed number, mitigate bald sharp phenomenon, Increase cob length.
2.2 cob length analysis
It analyzes the corn hybrid seed under normally for nitrogen condition (CK) and handles post-grouting period in different aminoacids state nitrogen Cob length.As a result, it has been found that arginine (T2) and glutamine (T3) process Zheng Dan 958 (ZD958), first jade 335 respectively (XY335), good beautiful 99 (LY99) and after stepping on 105 (DH105) of sea, cob length of all cenospecies in grouting period is significantly higher than It compares (T1), but influence indifference (as shown in Figure 9) of 2 kinds of amino acid to corncob length.
Also analyze less than normally for the nitrogen level condition (LN) of nitrogen under corn hybrid seed at different aminoacids state nitrogen Manage the cob length in post-grouting period.As a result, it has been found that arginine (T2) and glutamine (T3) process Zheng Dan 958 respectively (ZD958), first jade 335 (XY335), good beautiful 99 (LY99) and after stepping on 105 (DH105) of sea, all cenospecies are in the period of being in the milk Cob length is significantly higher than control (T1), but influence indifference (as shown in Figure 10) of 2 kinds of amino acid to corncob length.
2.3 mass of 1000 kernel are analyzed
It analyzes the corn hybrid seed under normally for nitrogen condition (CK) and handles post-grouting period in different aminoacids state nitrogen Mass of 1000 kernel.As a result, it has been found that arginine (T2) and glutamine (T3) process Zheng Dan 958 (ZD958), first jade 335 respectively (XY335), good beautiful 99 (LY99) and after stepping on 105 (DH105) of sea, mass of 1000 kernel of all cenospecies in grouting period are significantly higher than pair According to (T1), and glutamine processing increases 9.72%-30.38%, and it is 2.81%-16.03% (such as Figure 11 that arginine, which handles amplification, It is shown).
Also analyze less than normally for the nitrogen level condition (LN) of nitrogen under corn hybrid seed at different aminoacids state nitrogen Manage the mass of 1000 kernel in post-grouting period.As a result, it has been found that arginine (T2) and glutamine (T3) process Zheng Dan 958 respectively (ZD958), first jade 335 (XY335), good beautiful 99 (LY99) and after stepping on 105 (DH105) of sea, all cenospecies are in the period of being in the milk Mass of 1000 kernel is significantly higher than control (T1), and glutamine processing increases 9.44%-22.06%, and arginine processing amplification is 7.04%-11.08% (as shown in figure 12).
2.4 grain number per spikes are analyzed
It analyzes the corn hybrid seed under normally for nitrogen condition (CK) and handles post-grouting period in different aminoacids state nitrogen Grain number per spike.As a result, it has been found that arginine (T2) and glutamine (T3) process Zheng Dan 958 (ZD958), first jade 335 respectively (XY335), good beautiful 99 (LY99) and after stepping on 105 (DH105) of sea, grain number per spike of all cenospecies in grouting period are significantly higher than pair According to (T1), and glutamine processing increases 17.42%-40.62%, and it is that 7.79%-18.90% (such as schemes that arginine, which handles amplification, Shown in 13).
Also analyze less than normally for the nitrogen level condition (LN) of nitrogen under rice cenospecies different aminoacids state nitrogen processing The grain number per spike in post-grouting period.As a result, it has been found that arginine (T2) and glutamine (T3) respectively process Zheng Dan 958 (ZD958), First jade 335 (XY335), good beautiful 99 (LY99) and after stepping on 105 (DH105) of sea, grain number per spike of all cenospecies in the period of being in the milk is shown It writes higher than control (T1), in addition, glutamine processing increases 30.89%-54.61%, it is 21.92%- that arginine, which handles amplification, 28.13% (as shown in figure 14).
2.5 Ear weights are analyzed
It analyzes the corn hybrid seed under normally for nitrogen condition (CK) and handles post-grouting period in different aminoacids state nitrogen Ear weight.As a result, it has been found that arginine (T2) and glutamine (T3) process Zheng Dan 958 (ZD958), first jade 335 respectively (XY335), good beautiful 99 (LY99) and after stepping on 105 (DH105) of sea, Ear weight of all cenospecies in grouting period are significantly higher than pair According to (T1), and glutamine processing increases 31.13%-58.44%, and it is that 11.08%-42.44% (such as schemes that arginine, which handles amplification, Shown in 15).
Also analyze less than normally for the nitrogen level condition (LN) of nitrogen under corn hybrid seed at different aminoacids state nitrogen Manage the Ear weight in post-grouting period.As a result, it has been found that arginine (T2) and glutamine (T3) process Zheng Dan 958 respectively (ZD958), first jade 335 (XY335), good beautiful 99 (LY99) and after stepping on 105 (DH105) of sea, all cenospecies are in the period of being in the milk Ear weight is significantly higher than control (T1), in addition, glutamine processing increases 45.84%-88.56%, arginine processing amplification is 31.26%-37.67% (as shown in figure 16).
2.6 seed carotenoid concentrations are analyzed
It analyzes normally under nitrogen condition (CK), corn hybrid seed handles the seed in post-grouting period in different aminoacids state nitrogen Grain carotenoid concentration.As a result, it has been found that after arginine (T2) and glutamine (T3) process Zheng Dan 958 (ZD958) respectively, 1.65% and 13.06 are increased separately in the relatively control (T1) of grouting period seed carotenoid concentration, and arginine and glutamine First 335 (XY335) of jade do not improve its seed carotenoid concentration (as shown in figure 17) for processing.
Also analyze less than normally for the nitrogen level condition (LN) of nitrogen under corn hybrid seed at different aminoacids state nitrogen Manage the seed carotenoid concentration in post-grouting period.As a result, it has been found that glutamine (T3) processes Zheng Dan 958 (ZD958) respectively After first 335 (XY335) of jade, the relatively control (T1) of grouting period seed carotenoid concentration increases separately 13.11% He 8.02%, but arginine (T2) processing does not improve cenospecies seed carotenoid concentration (as shown in figure 18).
2.7 section nitrogen potentiality
According to following formula, (Chen Fanjun, meter Guo Hua, Zhang Fusuo wait the Beijing-tianjin-tangshan Areas part Summer Corn Varieties section nitrogen potentiality Estimation [J] Maize Sciences, 2009,17 (4):115-117.) calculate section nitrogen potentiality:
(1) the nitrogenous fertilizer agronomy efficiency of CK processing=(CK handles yield-LN and handles yield)/CK handles amount of nitrogen;
(2) processing section nitrogen quantity=CK controls amount of nitrogen-(CK control yield-
LN handle yield)/CK processing nitrogenous fertilizer agronomy efficiency;
(3) section nitrogen potentiality=processing section nitrogen quantity * 100/CK handle amount of nitrogen;
Wherein:
CK indicates the nitrogen (urea) using 200 kg/ha;
LN indicates the nitrogen using 0 kg/ha;
Processing indicates the arginine or glutamine using 1.25mM;
Control indicates not apply the arginine of 1.25mM or glutamine but sprays equivalent (volume is equivalent to aminoacids solution The volume of state fertilizer) water;
Then:
CK processing indicates the arginine or glutamy of nitrogen (urea) and application 1.25mM using 200 kg/ha Amine;
LN processing indicates the arginine or glutamine of nitrogen (urea) and application 1.25mM using 0 kg/ha;
CK controls indicate the nitrogen (urea) using 200 kg/ha and spray to be equivalent to amino acid fertilizer (containing smart ammonia Acid or glutamine) amount water;
CK processing amount of nitrogens include the N from nitrogen (urea) and the N from amino acid.
It can be seen that from section nitrogen potentiality correlation formula under conditions of reaching equal production volume, 2 kinds of amino acid processing are able to The nitrogen of saving.The result for saving nitrogen potentiality is as shown in figure 19, it can be seen that applying arginine or glutamine in four differences Nitrogenous fertilizer can be saved significantly in kind, can at least save 20% or more (LY99, arginine), it is highest to save nearly 90% (87.6%, XY335, glutamine).In addition, section nitrogen potentiality are variant between different cultivars, and the section nitrogen of glutamine Potentiality are above arginic section nitrogen potentiality in four different cultivars.
Finally it should be noted that:The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations;Although Present invention has been described in detail with reference to the aforementioned embodiments, it will be understood by those of ordinary skill in the art that:It still may be used With technical scheme described in the above embodiments is modified or equivalent replacement of some of the technical features; And these modifications or replacements, various embodiments of the present invention technical solution that it does not separate the essence of the corresponding technical solution spirit and Range.

Claims (10)

1. a kind of amino acid liquid fertilizer, which is characterized in that the amino acid liquid fertilizer includes the water of amino acid and surplus, the ammonia Base acid is arginine or glutamine;Preferably, a concentration of 0.5mM to 2.5mM of the amino acid.
2. a kind of corn planting method, which is characterized in that the method includes:It is sprayed to corn comprising 0.5mM to 2.5mM amino The amino acid liquid fertilizer of acid, the amino acid are arginine and/or glutamine.
3. according to the method described in claim 1, it is characterized in that, the amino acid liquid fertilizer includes:Arginine and/or paddy ammonia Amide;With the water of surplus.
4. according to the method described in claim 1, it is characterized in that, described spray carries out in one or more following period: 6th leaf is fully deployed the phase, the 8th leaf is fully deployed the phase, the 12nd leaf is fully deployed the phase or the spinning phase.
5. according to the method described in claim 1, it is characterized in that, the amount sprayed is 250g/hm2To 350g/hm2Essence Propylhomoserin or 200g/hm2To 300g/hm2Glutamine.
6. according to the method described in claim 1, it is characterized in that, the method further includes providing 0 to 200kg/hm2Nitrogenous fertilizer.
7. the method according to any one of claim 2 to 6, which is characterized in that the corn is hybrid maize;Preferably It is that the hybrid maize is selected from the group being made of first beautiful 335, Zheng Dan 958, good beautiful 99 and Deng Hai 605.
8. application of the amino acid in improving corn grouting effect, which is characterized in that the amino acid is arginine and/or paddy ammonia Amide.
9. application according to claim 8, which is characterized in that the improvement corn grouting effect include as next or The improvement of multiple indexs:Corncob phenotype, mass of 1000 kernel, Ear weight, grain number per spike, seed carotenoid concentration;Preferably, institute It states corncob phenotype and is selected from the group being made of grain plumpness, cob length, kernal number and bald sharp phenomenon.
10. application according to claim 9, which is characterized in that the improvement corn grouting effect includes:
Corncob phenotype improves, including grain plumpness improves, cob length increases, kernal number increases and/or bald sharp phenomenon subtracts Gently;
Mass of 1000 kernel increases, it is preferred that mass of 1000 kernel increases by 2.81% to 22.06%;
Grain number per spike increases, it is preferred that grain number per spike increases by 7.79% to 54.61%;
Ear weight increases, it is preferred that Ear weight increases by 11.08% to 88.56%;And/or
Seed carotenoid concentration increases, it is preferred that seed carotenoid concentration increases by 1.65% to 13.11%.
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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106818134A (en) * 2016-12-30 2017-06-13 吕福贵 A kind of implantation methods of corn
CN107628853A (en) * 2017-11-10 2018-01-26 太原理工大学 A kind of growth period corn special bio fertilizer and preparation method thereof
CN107805105A (en) * 2017-12-12 2018-03-16 凤阳县板桥镇金祥种植专业合作社 A kind of corn pustulation period dedicated fertilizer

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106818134A (en) * 2016-12-30 2017-06-13 吕福贵 A kind of implantation methods of corn
CN107628853A (en) * 2017-11-10 2018-01-26 太原理工大学 A kind of growth period corn special bio fertilizer and preparation method thereof
CN107805105A (en) * 2017-12-12 2018-03-16 凤阳县板桥镇金祥种植专业合作社 A kind of corn pustulation period dedicated fertilizer

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Title
杨洪强 等: "植物精氨酸及其代谢产物的生理功能", 《植物生理与分子生物学学报》 *

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