CN108588885A - A kind of sub-micron fibers and preparation method thereof containing medicine-carried nanospheres - Google Patents
A kind of sub-micron fibers and preparation method thereof containing medicine-carried nanospheres Download PDFInfo
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- D—TEXTILES; PAPER
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- D01F6/00—Monocomponent artificial filaments or the like of synthetic polymers; Manufacture thereof
- D01F6/44—Monocomponent artificial filaments or the like of synthetic polymers; Manufacture thereof from mixtures of polymers obtained by reactions only involving carbon-to-carbon unsaturated bonds as major constituent with other polymers or low-molecular-weight compounds
- D01F6/54—Monocomponent artificial filaments or the like of synthetic polymers; Manufacture thereof from mixtures of polymers obtained by reactions only involving carbon-to-carbon unsaturated bonds as major constituent with other polymers or low-molecular-weight compounds of polymers of unsaturated nitriles
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- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
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- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/32—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers, poly(meth)acrylates, or polyvinyl pyrrolidone
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
- A61K47/6949—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit inclusion complexes, e.g. clathrates, cavitates or fullerenes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0092—Hollow drug-filled fibres, tubes of the core-shell type, coated fibres, coated rods, microtubules or nanotubes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/51—Nanocapsules; Nanoparticles
- A61K9/5107—Excipients; Inactive ingredients
- A61K9/513—Organic macromolecular compounds; Dendrimers
- A61K9/5138—Organic macromolecular compounds; Dendrimers obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyvinyl pyrrolidone, poly(meth)acrylates
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/51—Nanocapsules; Nanoparticles
- A61K9/5107—Excipients; Inactive ingredients
- A61K9/513—Organic macromolecular compounds; Dendrimers
- A61K9/5161—Polysaccharides, e.g. alginate, chitosan, cellulose derivatives; Cyclodextrin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
- D01F1/103—Agents inhibiting growth of microorganisms
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Abstract
The present invention relates to a kind of sub-micron fibers and preparation method thereof containing medicine-carried nanospheres, material are the SiO for containing load triclosan2The sub-micron fibers of/CS/PAA nano-particles.To prepare include the preparation of hollow mesoporous SiO2, loads triclosan the preparation of SiO2 nano-particles, the preparation of SiO2/CS nano-particles for loading triclosan, load triclosan SiO2/CS/PAA nano-particles preparation and electrostatic spinning step containing silica medicine-carried nanosphere fiber.The technique preparation route of the present invention is simple, reaction condition is mild, at low cost, and nontoxic.
Description
Technical field
The invention belongs to sub-micron fibers material and its preparation field, more particularly to a kind of sub-micro containing medicine-carried nanospheres
Rice fiber and preparation method thereof.
Background technology
In nanometer medicine-carried system, drug molecule is typically uniformly dispersed throughout in carrier matrix or is adsorbed in carrier surface, obtains
Carrier-drug complex in addition to play the role of carry drug other than, can also influence and control the release dynamics of drug.Often
The nano-medicament carrier seen includes nanoparticle, nanofiber, Nano capsule, nano liposomes etc..Nano-medicament carrier its compared with
High specific surface area can load higher medication amount；And the Nano medication that can prepare varying environment stimulating responsive carries
Body, the features such as making it have biodegradability, temperature sensitivity, PH sensibility, is to Drug controlled release；Also medicine can be improved
The effective rate of utilization of object simultaneously reduces toxic side effect of the drug to cell.
Nanofiber is controllable with surface topography, the easy modification in surface, load medicine is easy to operate, it is smaller to be influenced on medicine effect
Etc. a series of advantage.Include mainly conventional electrostatic spin processes by the method that electrostatic spinning technique prepares pharmaceutical carrier, coaxial quiet
Electrospinning and lotion Static Spinning etc..Conventional electrostatic is spun simply mixes drug with Polymer Solution, with then will being mixed with drug
Polymer Solution direct fabrics.Drug is usually evenly spread in fiber, forms drug-matrix structure.In this structure, drug and
Between matrix active force it is not strong, be frequently accompanied by the generation of burst drug release phenomenon, and with the extension of time, drug release rate drops
It is low.
Hollow meso-porous nano silica is a kind of ideal nano-medicament carrier, there is the specific surface area of superelevation, prepares letter
It is single, and containing active silicone hydroxyl, can be by drug loading inside its pore passage structure as pharmaceutical carrier, while drug molecule
It can also be adsorbed on meso-porous titanium dioxide silicon face by power the effects that hydrogen bond, electrostatic interaction.Meanwhile hollow mesoporous silicon oxide tool
There are good biocompatibility and the non-toxic safety for ensuring it as slow releasing carrier of medication.
Hybridized fiber typically refers to the nanofiber of different classes of material composition, such as polymer/inorganic nanometer particle hydridization
Fiber etc..Hybridized fiber would generally show more excellent performance, such as machine capability improves, drug wraps up ability and cell
Compatibility enhancing improvement etc..Hybridized fiber is with a wide range of applications in fields such as organizational project, catalyst and pharmaceutical carriers.
Invention content
Technical problem to be solved by the invention is to provide a kind of sub-micron fibers containing medicine-carried nanospheres and its preparations
Method, preparation method of the present invention is easy to operate, there is good market prospects.
A kind of sub-micron fibers containing medicine-carried nanospheres of the present invention, it is characterised in that：The material is to contain load
The SiO of triclosan2The sub-micron fibers of/CS/PAA nano-particles.
The SiO of the load triclosan2/ CS/PAA nano-particles are：Triclosan be supported on the hollow mesoporous ducts SiO2 and
Then surface coats the nano-particle that chitosan and polyacrylic acid are formed.
A kind of preparation method of sub-micron fibers containing medicine-carried nanospheres of the present invention, including：
(1) by hollow mesoporous SiO2It is distributed in Triclosan solution, stirs 36-72h at room temperature, centrifuge, washing, vacuum is done
It is dry, obtain the SiO2 nano-particles of load triclosan；
(2) the SiO2 nano-particles of above-mentioned load triclosan are dispersed in chitosan solution, stir 36-72h at room temperature,
Centrifugation is washed, and vacuum drying obtains the SiO of load triclosan2/ CS nano-particles；
(3) by the SiO of above-mentioned load triclosan2/ CS nano-particles are placed in polyacrylic acid PAA solution, are stirred at room temperature
After 36-72h, centrifugation, washing, vacuum drying obtain the SiO2/CS/PAA nucleocapsid composite nano anti-biotic particles of load triclosan；
(4) the SiO2/CS/PAA nucleocapsid composite nano anti-biotic particles for loading triclosan are dispersed in polyacrylonitrile (PAN) solution
In, electrostatic spinning is carried out to get the sub-micron fibers containing medicine-carried nanospheres.
Hollow mesoporous SiO in step (1)2Preparation method be specially：PAA is added in ammonium hydroxide, after stirring evenly, is added
Enter absolute ethyl alcohol, after stirring 1h at room temperature, ethyl orthosilicate is added, at room temperature after sealing stirring 12h, acquired solution centrifuges, is clear
It washes, dry, obtain hollow mesoporous SiO2；Wherein polyacrylic acid PAA, ammonium hydroxide, absolute ethyl alcohol, ethyl orthosilicate proportionate relationship be
0.15~0.2g：4~4.5mL：80~90mL：1.2~1.5mL.
The solvent of Triclosan solution is ethyl alcohol in step (1).
The solvent of chitosan solution is acetic acid in step (2), and the mass fraction of acetic acid is 1%.
Load the SiO of triclosan2Triclosan, chitosan, polyacrylic acid in/CS/PAA nucleocapsid composite nano anti-biotic particles
The mass ratio of PAA is 1:2:1~1.5.
The specification of polyacrylonitrile (PAN) is Mw=85000 in the step (4)；The solvent of polyacrylonitrile (PAN) solution is N, N-
Dimethylformamide DMF.
The mass percent of the polyacrylonitrile (PAN) solution is 8-12wt%.
Electrostatic spinning process is in the step (4)：It is 10~18KV to apply voltage in electrostatic spinning, and it is 10 to receive distance
~15cm, solution flow rate are 0.6~1.0mL/h.
Hollow mesoporous silicon oxide and electro spinning nano fiber are all pharmaceutical carriers in the present invention, and the two hydridization is applied simultaneously
In drug release.Triclosan is supported on the hollow mesoporous ducts SiO2 and surface, while loading the surfaces SiO2 after triclosan simultaneously
Cladding chitosan and polyacrylic acid, triclosan have obtained three-layer protection, CS and PAA under varying environment pH value, degree of ionization
It has differences, pH value stimulation and bacterium stimulation can be responded simultaneously, alleviate burst drug release problem；On the other hand, it will carry in medicine
Empty mesoporous silicon oxide is placed among nanofiber, has better slow release effect and long-acting performance.In addition, the technique of the present invention
Preparation route is simple, reaction condition is mild, at low cost, and nontoxic.
Advantageous effect
(1) technique preparation route of the invention is simple, reaction condition is mild, at low cost, and nontoxic；
(2) present invention synthesizes composite nano intelligent antimicrobial particle structure novel, antiseptic can according to environment pH value and
The stimulation of bacterium is sustained, and can be widely used in the fields such as biological, medical, weaving.
Description of the drawings
Fig. 1 is the FE-SEM figures of hollow mesoporous silicon oxide prepared by embodiment 1.
Fig. 2 is the TEM figures of hollow mesoporous silicon oxide prepared by embodiment 1.
Fig. 3 is that the hollow mesoporous electrostatic spinning sub-micron for carrying medicine silica nanosphere containing load prepared by embodiment 1 is fine
The SEM of dimension schemes.
Fig. 4 is the SiO of load triclosan prepared by embodiment 12And SiO2/ CS/PAA and load SiO2The nanometer of/CS/PAA
Fiber carries out sustained release behavior curve graph.
Specific implementation mode
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.In addition, it should also be understood that, after reading the content taught by the present invention, people in the art
Member can make various changes or modifications the present invention, and such equivalent forms equally fall within the application the appended claims and limited
Range.
Embodiment 1
(1) hollow mesoporous SiO2Preparation：4.3ml ammonium hydroxide is added into 0.15g polyacrylic acid aqueous solutions, stirs evenly
Afterwards, 87ml absolute ethyl alcohols are added, after stirring 1h at room temperature, 1.4ml ethyl orthosilicates are added dropwise, continue sealing stirring at room temperature
12h.By acquired solution centrifugation, cleaning 5 times, drying in 50 DEG C of baking oven is placed, hollow mesoporous silicon oxide is obtained, is disperseed
It in ethanol solution, is respectively placed on silicon chip and carbon film, is used for Flied emission and transmission, test result is respectively such as Fig. 1 and Fig. 2 institutes
Show, it can be seen that nano particle shape is more regular, and size is less uniform, there is apparent hollow meso-hole structure.
(2) SiO of triclosan is loaded2The preparation of nano-particle：1.6g triclosans are scattered in 60ml absolute ethyl alcohols, room
Temperature is lower to stir 1h, and triclosan is made to be completely dissolved.It weighs the hollow mesoporous silicon oxides of 0.8g to be scattered in the solution, seal at room temperature
Solution centrifugation, cleaning after reaction is put in drying in 50 DEG C of baking oven, obtains the SiO of load triclosan by stirring 72 hours2
Nano-particle.
(3) SiO of triclosan is loaded2The preparation of/CS nano-particles：0.8g chitosans are dissolved in 1% acetic acid solution,
It stirs at room temperature 24 hours, it is 6 then to utilize the NaOH solution of 1M to adjust pH value, and it is poly- that the solid obtained in (2) is scattered in shell
It in sugar juice, stirs 72 hours, the solution after reaction is centrifuged, cleaning at room temperature, be dried in vacuo at 50 DEG C and obtain load trichlorine
Raw SiO2/CS nano-particles.
(4) SiO of triclosan is loaded2The preparation of/CS/PAA nano-particles：1g polyacrylic acid aqueous solutions are dissolved in 40ml
It in ionized water, stirs 1 hour at room temperature, it is 6 to adjust pH value using the NaOH solution of 1M, and the solid obtained in (3) is scattered in
It in the aqueous solution of polyacrylic acid, stirs 72 hours, the solution after reaction is centrifuged, cleaning at room temperature, is dried in vacuo at 50 DEG C
To the SiO of load triclosan2/ CS/PAA nano-particles.
(5) PAN is dissolved in DMF, configuration quality percentage is the PAN solution of 10wt%.
(6) SiO for the load triclosan for preparing step (4)2/ CS/PAA nano-particles evenly spread to step (5)
In solution, at room temperature after ultrasound 4h, electrostatic spinning is carried out, application voltage is 16KV, and it is 12cm to receive distance, and solution flow rate is
The nano fibrous membrane of 0.8ml/h, preparation are used for sem test, and the results are shown in Figure 3, can learn that fibre fineness is more equal
It is even, a no beading.By the SiO of the load triclosan of preparation2And SiO2/ CS/PAA and load SiO2The nanofiber of/CS/PAA carries out
The research of sustained release behavior, the results are shown in Figure 4 for sustained release, it can be seen that load SiO2The nanofiber ratio SiO of/CS/PAA2/CS/
PAA and SiO2With better slow release effect.
Embodiment 2
(1) hollow mesoporous SiO2Preparation：4.5ml ammonium hydroxide is added into 0.17g polyacrylic acid aqueous solutions, stirs evenly
Afterwards, 88ml absolute ethyl alcohols are added, after stirring 1h at room temperature, 1.7ml ethyl orthosilicates are added dropwise, continue sealing stirring at room temperature
12h.By acquired solution centrifugation, cleaning 5 times, drying in 50 DEG C of baking oven is placed, hollow mesoporous silicon oxide is obtained, is disperseed
It in ethanol solution, is respectively placed on silicon chip and carbon film, is used for Flied emission and transmission, test result is respectively such as Fig. 1 and Fig. 2 institutes
Show, it can be seen that nano particle shape is more regular, and size is less uniform, there is apparent hollow meso-hole structure.
(2) SiO of triclosan is loaded2The preparation of nano-particle：1.6g triclosans are scattered in 60ml absolute ethyl alcohols, room
Temperature is lower to stir 1h, and triclosan is made to be completely dissolved.It weighs the hollow mesoporous silicon oxides of 1.0g to be scattered in Triclosan solution, at room temperature
Solution centrifugation, cleaning after reaction is put in drying in 50 DEG C of baking oven by sealing stirring 72 hours, obtains load triclosan
SiO2Nano-particle.
(3) SiO of triclosan is loaded2The preparation of/CS nano-particles：0.8g chitosans are dissolved in 1% acetic acid solution,
It stirs at room temperature 24 hours, it is 6 then to utilize the NaOH solution of 1M to adjust pH value, and it is poly- that the solid obtained in (2) is scattered in shell
It in sugar juice, stirs 72 hours, the solution after reaction is centrifuged, cleaning at room temperature, be dried in vacuo at 50 DEG C and obtain load trichlorine
Raw SiO2/CS nano-particles.
(4) SiO of triclosan is loaded2The preparation of/CS/PAA nano-particles：1g polyacrylic acid aqueous solutions are dissolved in 40ml
It in ionized water, stirs 1 hour at room temperature, it is 6 to adjust pH value using the NaOH solution of 1M, and the solid obtained in (3) is scattered in
It in the aqueous solution of polyacrylic acid, stirs 72 hours, the solution after reaction is centrifuged, cleaning at room temperature, is dried in vacuo at 50 DEG C
To the SiO of load triclosan2/ CS/PAA nano-particles.
(5) PAN is dissolved in DMF, configuration quality score is the PAN solution of 10wt%.
(6) SiO for the load triclosan for preparing step (4)2/ CS/PAA nano-particles evenly spread to step (5)
In solution, at room temperature after ultrasound 4h, electrostatic spinning is carried out, application voltage is 16KV, and it is 12cm to receive distance, and solution flow rate is
0.8ml/h obtains the SiO of the triclosan containing load2The electrostatic spinning sub-micron of/CS/PAA nucleocapsid composite nano anti-biotic particles is fine
Dimension.
Embodiment 3
(1) hollow mesoporous SiO2Preparation：4.5ml ammonium hydroxide is added into 0.18g polyacrylic acid aqueous solutions, stirs evenly
Afterwards, 90ml absolute ethyl alcohols are added, after stirring 1h at room temperature, 1.5ml ethyl orthosilicates are added dropwise, continue sealing stirring at room temperature
12h.By acquired solution centrifugation, cleaning 5 times, drying in 50 DEG C of baking oven is placed, hollow mesoporous silicon oxide is obtained, is disperseed
It in ethanol solution, is respectively placed on silicon chip and carbon film, is used for Flied emission and transmission, test result is respectively such as Fig. 1 and Fig. 2 institutes
Show, it can be seen that nano particle shape is more regular, and size is less uniform, there is apparent hollow meso-hole structure.
(2) SiO of triclosan is loaded2The preparation of nano-particle：1.6g triclosans are scattered in 60ml absolute ethyl alcohols, room
Temperature is lower to stir 1h, and triclosan is made to be completely dissolved.It weighs the hollow mesoporous silicon oxides of 0.8g to be scattered in the solution, seal at room temperature
Solution centrifugation, cleaning after reaction is put in drying in 50 DEG C of baking oven, obtains the SiO of load triclosan by stirring 72 hours2
Nano-particle.
(3) SiO of triclosan is loaded2The preparation of/CS nano-particles：0.8g chitosans are dissolved in 1% acetic acid solution,
It stirs at room temperature 24 hours, it is 6 then to utilize the NaOH solution of 1M to adjust pH value, and it is poly- that the solid obtained in (2) is scattered in shell
It in sugar juice, stirs 72 hours, the solution after reaction is centrifuged, cleaning at room temperature, be dried in vacuo at 50 DEG C and obtain load trichlorine
Raw SiO2/CS nano-particles.
(4) SiO of triclosan is loaded2The preparation of/CS/PAA nano-particles：1g polyacrylic acid aqueous solutions are dissolved in 40ml
It in ionized water, stirs 1 hour at room temperature, it is 6 to adjust pH value using the NaOH solution of 1M, and the solid obtained in (3) is scattered in
It in the aqueous solution of polyacrylic acid, stirs 72 hours, the solution after reaction is centrifuged, cleaning at room temperature, is dried in vacuo at 50 DEG C
To the SiO of load triclosan2/ CS/PAA nano-particles.
(5) PAN is dissolved in DMF, configuration quality score is the PAN solution of 10wt%.
(6) SiO for the load triclosan for preparing step (4)2/ CS/PAA nano-particles evenly spread to step (5)
In solution, at room temperature after ultrasound 4h, electrostatic spinning is carried out, application voltage is 16KV, and it is 12cm to receive distance, and solution flow rate is
0.8ml/h obtains the SiO of the triclosan containing load2The electrostatic spinning sub-micron of/CS/PAA nucleocapsid composite nano anti-biotic particles is fine
Dimension.
Claims (9)
1. a kind of sub-micron fibers containing medicine-carried nanospheres, it is characterised in that：The material is containing load triclosan
SiO2The sub-micron fibers of/CS/PAA nano-particles.
2. a kind of sub-micron fibers containing medicine-carried nanospheres according to claim 1, it is characterised in that：The load three
The SiO of chlorine life2/ CS/PAA nano-particles are：Triclosan is supported on the hollow mesoporous ducts SiO2 and surface, then coats chitosan
The nano-particle formed with polyacrylic acid.
3. a kind of preparation method of the sub-micron fibers containing medicine-carried nanospheres as described in claim 1, including：
(1) by hollow mesoporous SiO2It is distributed in Triclosan solution, stirs 36-72h at room temperature, centrifuge, wash, vacuum drying obtains
To the SiO2 nano-particles of load triclosan；
(2) the SiO2 nano-particles of above-mentioned load triclosan are dispersed in chitosan CS solution, stir 36-72h at room temperature, from
The heart washs, and vacuum drying obtains the SiO of load triclosan2/ CS nano-particles；
(3) by the SiO of above-mentioned load triclosan2/ CS nano-particles are placed in polyacrylic acid PAA aqueous solutions, stir 36- at room temperature
After 72h, centrifugation, washing, vacuum drying obtain the SiO2/CS/PAA nucleocapsid composite nano anti-biotic particles of load triclosan；
(4) the SiO2/CS/PAA nucleocapsid composite nano anti-biotic particles for loading triclosan are dispersed in polyacrylonitrile (PAN) solution,
Electrostatic spinning is carried out to get the sub-micron fibers containing medicine-carried nanospheres.
4. a kind of preparation method of sub-micron fibers containing medicine-carried nanospheres according to claim 3, it is characterised in that：
The solvent of Triclosan solution is ethyl alcohol in step (1).
5. a kind of preparation method of sub-micron fibers containing medicine-carried nanospheres according to claim 3, it is characterised in that：
The solvent of chitosan solution is acetic acid in step (2), and the mass fraction of acetic acid is 1%.
6. a kind of preparation method of sub-micron fibers containing medicine-carried nanospheres according to claim 3, it is characterised in that：
Load triclosan SiO2/CS/PAA nucleocapsid composite nano anti-biotic particles in triclosan, chitosan, polyacrylic acid mass ratio be
1:2:1~1.5.
7. a kind of preparation method of sub-micron fibers containing medicine-carried nanospheres according to claim 3, it is characterised in that：
The specification of polyacrylonitrile (PAN) is Mw=85000 in the step (4)；The solvent of PAN solution is N,N-dimethylformamide DMF.
8. a kind of preparation method of sub-micron fibers containing medicine-carried nanospheres according to claim 3, it is characterised in that：
The mass percent of the polyacrylonitrile (PAN) solution is 8-12wt%.
9. a kind of preparation method of sub-micron fibers containing medicine-carried nanospheres according to claim 3, it is characterised in that：
Electrostatic spinning process is in the step (4)：It is 10~18KV to apply voltage in electrostatic spinning, and it is 10~15cm to receive distance,
Solution flow rate is 0.6~1.0mL/h.
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| CN201810394569.9A CN108588885A (en) | 2018-04-27 | 2018-04-27 | A kind of sub-micron fibers and preparation method thereof containing medicine-carried nanospheres |
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| CN111821463A (en) * | 2020-07-21 | 2020-10-27 | 中国矿业大学 | Preparation method of drug-loaded polycaprolactone-chitosan-silicon dioxide hybrid fiber |
| CN114592277A (en) * | 2022-03-09 | 2022-06-07 | 诺一迈尔(苏州)生命科技有限公司 | Strontium-doped silicon dioxide nanofiber membrane and preparation method thereof |
| CN114606767A (en) * | 2022-04-12 | 2022-06-10 | 福州大学 | Preparation method of broad-spectrum antibacterial polypropylene non-woven fabric |
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| CN111821463B (en) * | 2020-07-21 | 2021-10-15 | 中国矿业大学 | Preparation method of drug-loaded polycaprolactone-chitosan-silicon dioxide hybrid fiber |
| CN114592277A (en) * | 2022-03-09 | 2022-06-07 | 诺一迈尔(苏州)生命科技有限公司 | Strontium-doped silicon dioxide nanofiber membrane and preparation method thereof |
| CN114606767A (en) * | 2022-04-12 | 2022-06-10 | 福州大学 | Preparation method of broad-spectrum antibacterial polypropylene non-woven fabric |
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