CN108572230A - Detect the on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in blood - Google Patents
Detect the on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in blood Download PDFInfo
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- G—PHYSICS
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- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
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Abstract
The on-line solid phase extraction liquid phase chromatography analytical method that the present invention detects content of valproic acid in blood is demarcated to standard solution using high performance liquid chromatograph and DAD detectors,It is y=a*x+b that fitting, which obtains calibration curve equation,,Take blood sample to be measured,The blood to be detected after treatment,Equally sample to be measured is detected using high performance liquid chromatograph and DAD detectors,Obtain blood y values to be measured,Blood y to be measured is substituted into calibration curve equation,Valproic acid relative concentration x in blood sample to be measured is obtained by calculation,Internal standard compound working solution concentration is known,Thus the valproic acid drug concentration in blood to be detected in the sample is calculated,The present invention is diluted serum with the PES filter membranes of 0.45um,Improve the accuracy of quantitative result,Greatly shorten analysis time,The generation for reducing valproic acid toxicity provides experiment basis.
Description
Technical field
The present invention relates in the clinical therapeutic drug monitoring technical field of antipsychotics more particularly to a kind of detection blood
The on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid.
Background technology
Epilepsy is common the nervous system disease, have suddenly, recurrent exerbation the characteristics of, clinically choice drug is controlled at present
It treats.Sodium vedproate is traditional wide spectrum antiepileptic, effective to all types of epilepsies.After it is oral by gastrointestinal tract after,
The reactive compound recycled in blood is valproic acid.Valproic acid (Valproic acid, VPA), molecular formula C8H16O2, specifically
Chemical constitution is illustrated in fig. 1 shown below.The effective blood drug concentration of valproic acid is 50-100mg/L, as blood concentration ＞ 100mg/L, poison
Side effect enhances.Common adverse reactions are gastrointestinal reactions, and hepatotoxicity wind agitation occurs in small number of patients.VPA is mainly sent out by nervous system
Its pharmacological action is waved, since VPA therapeutic windows are relatively narrow, for internal metabolic process there are larger individual difference, medicining cycle is long, administration
Dosage and blood concentration correlation are unstable, and blood concentration is related to curative effect and toxic reaction, and dosage is insufficient and drug
Excessive clinical symptoms are similar, so being adjustment dosage to the monitoring of its blood concentration, reduce the important references of adverse reaction,
It is of great significance in clinical application.
The assay method of currently used valproic acid (sodium) blood concentration is numerous, respectively has advantage and disadvantage, including fluorescence polarization is exempted from
Epidemic disease analytic approach, enzyme amplification immunization, high performance liquid chromatography, gas chromatography, liquid chromatography tandem mass spectrometry etc..Fluorescence polarization
Immunoassay high sensitivity, it is fast and convenient, but analysis cost is very high, is easily interfered by other substances.Enzyme amplifies immunization certainly
Dynamicization degree is high, and sample dosage is few, but stabilization of kit is poor, and specificity is poor, expensive.Liquid Chromatography-Tandem Mass Spectrometry
Method, which measures blood concentration, has many advantages, such as that high sensitivity, specificity are strong, but cost is higher, and the requirement to personnel and environment compared with
It is high.High performance liquid chromatography quantifies accurate, favorable reproducibility, and good in economic efficiency, but at present there are still complex pretreatment, when analysis
Between it is longer the problems such as.
Invention content
In view of the above technical problems, it is an object of the present invention to provide a kind of online solid phase extractions of content of valproic acid in detection blood
Liquid phase chromatography analytical method is taken, the measurement for making Valproic Acid in Serum quick and precisely, largely shortens the sample analysis time.
The technical solution adopted by the present invention is：
The on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in a kind of detection blood of the present invention, wherein：
It includes the following steps：
(1) calibration of standard solution
First with liquid-transfering gun by 10 μ L of standard working solution of at least three kinds various concentrations, 10 μ L standards internal standard solutions and 190 μ L
Blank serum, which is respectively placed in 1.5ml centrifuge tubes, is mixed and made at least three kinds of standard solution, and above-mentioned standard solution is respectively in rotating speed
After vortex mixing 30s-1mmin under 1000-2000rpm, 200 μ of diluent containing 0.4-0.6% high chloro acid solutions is added
L, and be filtered after rotating speed is vortex mixing 2-4min under 1200-2000rpm, then with the PES filter membranes of 0.45um, it obtains
Clear liquid takes above-mentioned supernatant 200uL, is detected to above-mentioned supernatant with high performance liquid chromatograph and DAD detectors respectively, point
The valproic acid chromatogram and caprylic acid chromatogram in above-mentioned at least three kinds of standard solution are not obtained, it is molten with above-mentioned at least three standard
The ordinate y of the ratio between valproic acid peak area and caprylic acid peak area of liquid as canonical plotting, in above-mentioned standard working solution
Abscissa x in concentration containing valproic acid and standard internal standard solution containing the ratio between caprylic acid concentration as canonical plotting, will be with
At least three groups of data carry out linear regression obtained by upper detection, and it is y=a*x+b that fitting, which obtains calibration curve equation, and obtains power
Weight coefficient a and b, standard working solution is the solution containing valproic acid, and standard internal standard solution is the solution containing caprylic acid；
(a) preparation of standard working solution：
Accurately weighing valproic acid standard items 45mg is placed in 1ml volumetric flasks, is dissolved with methanol, and constant volume is obtained in 1ml
Standard reserving solution A, a concentration of 45000mg/L of valproic acid, the methanol for being 10%-40% with water content by standard reserving solution A
The dilution of solution is diluted, and configures the standard of each concentration in the range of containing 400-4000 μ g/mL valproic acids respectively
Working solution, and preserved under the conditions of -80 DEG C；
(b) preparation of standard internal standard solution
Caprylic acid standard items 210.79mg accurately is weighed in 2mL test tubes, is dissolved with 2mL methanol, is obtained internal standard storing solution
B, a concentration of 105395mg/L of caprylic acid, by the dilution of the internal standard storing solution B methanol solutions for being 10%-40% with water content
It is diluted, obtains the standard internal standard solution containing a concentration of 10500mg/L of caprylic acid, and preserved under the conditions of -80 DEG C,
(2) centrifugation of blood is detected
Blood to be detected at least 5ml is taken, 10min is centrifuged in the case where centrifugal speed is 3500rpm, obtains supernatant i.e. serum,
It is spare to before analyzing that above-mentioned serum is placed in the lower preservation of -20 DEG C of freezings；
(3) sample to be tested is handled
(c) it uses the standard internal standard solution 10uL of liquid-transfering gun removing step (b) in the centrifuge tube of 1.5ml, step is then added
(2) serum of 200uL centrifuges 30s-1min in above-mentioned centrifuge tube in the case where centrifugal speed is 1500-2500rpm；Then it uses
Liquid-transfering gun pipettes the 200 μ L of diluent containing 0.4-0.6% high chloro acid solutions and is added in above-mentioned centrifuge tube, then with 0.45um's
Filter membrane is filtered, and it is sample to be tested to obtain supernatant；
(4) detection of sample to be tested
Removing step (c) sample to be tested 200uL, using high performance liquid chromatograph and DAD detectors to above-mentioned sample to be tested
It is detected, the valproic acid chromatogram and caprylic acid chromatogram of above-mentioned sample to be tested is obtained, by the valproic acid in above-mentioned chromatogram
The ratio between peak area and caprylic acid peak area y are substituted into the calibration curve equation y=a*x+b of above-mentioned steps (one), by calculating
To the ratio between caprylic acid concentration in valproic acid concentration in detected sample and standard internal standard solution x, caprylic acid concentration in standard internal standard solution
It is known, the valproic acid drug concentration in blood to be detected is obtained by calculation；
The on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in a kind of detection blood of the present invention, wherein：
It is respectively to contain that the standard working solution of seven kinds of various concentrations, the standard working solution of seven kinds of various concentrations are used in step (1)
400, the valproic acid solution of 800,1200,1600,2000,3000 and 4000 μ g/mL concentration；
The on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in a kind of detection blood of the present invention, wherein：
The precipitating reagent is containing 0.5% high chloro acid solution；
The on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in a kind of detection blood of the present invention, wherein：
The dilution that dilution in step (a) and (b) is made of 3: 7 water and methanol；
The on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in a kind of detection blood of the present invention, wherein：
The high performance liquid chromatograph further includes：On-line solid phase extraction device, extraction column used in on-line solid phase extraction device are
Thermo TurboFlow C18 XL；
The on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in a kind of detection blood of the present invention, wherein：
Analysis chromatographic column used in the high performance liquid chromatograph is Agilent Extend-C18；Used pot strainer is
The column temperature of 1/16 0.5M of SSI COL PRE-FILTER WATER, high performance liquid chromatograph setting are 50 DEG C, and sample size is
100uL；
The on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in a kind of detection blood of the present invention, wherein：
The extraction column mobile phase of the on-line solid phase extraction instrument is the water containing 0.015% phosphoric acid and 10mmol/L sodium dihydrogen phosphates, extraction
It is 1.5mL/min to take column flow rate, and extraction column uses recoil pattern；
The on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in a kind of detection blood of the present invention, wherein：
The analysis column Mobile phase contains the organic phase of 22: 78 ratios：Water phase, wherein organic phase be acetonitrile, water phase be containing
The water of 0.015% phosphoric acid and 10mmol/L sodium dihydrogen phosphates, analysis column flow rate are 1.0mL/min, and analysis chromatographic column uses
Isocratic elution mode；
The on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in a kind of detection blood of the present invention, wherein：
The DAD detectors are Vanquish diode array detector, Detection wavelength 212nm；
The on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in a kind of detection blood of the present invention, wherein：
The water content is the water content of volume ratio.
Advantageous effect of the present invention：
The on-line solid phase extraction liquid phase analysis method of valproic acid drug concentration in detection blood of the present invention, serum warp
Direct injected after film was diluted, reduces personnel's operation, improves the accuracy of quantitative result, greatly shorten analysis time,
Keep detection process easy quickly, experimental cost reduces, to the Valproic Acid in Serum of patient's body more conducively in clinical treatment
It is monitored, experiment basis is provided for Personalized Drug Administration, the reduction toxicity of valproic acid.
Description of the drawings
Fig. 1 is valproic acid chemical structural formula；
Fig. 2 is valproic acid chromatogram in embodiment Plays solution；
Fig. 3 is valproic acid chromatogram in mark-on serum sample in embodiment.
In figure 2 and figure 3, mark 1 is valproic acid peak area, and mark 2 is caprylic acid peak area.
Below in conjunction with specific embodiments and the drawings, the invention will be further described.
Specific implementation mode
The on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in a kind of detection blood of the present invention, it includes
Following steps：
(1) calibration of standard solution
First with liquid-transfering gun by 10 μ L of standard working solution of seven kinds of various concentrations, 10 μ L standards internal standard solutions and 190 μ L blank
Serum, which is respectively placed in 1.5ml centrifuge tubes, is mixed and made into seven kinds of standard solution, and above-mentioned standard solution is respectively 2000rpm in rotating speed
After lower vortex mixing 30s, the 200 μ L of diluent containing 0.5% high chloro acid solution, and the whirlpool in the case where rotating speed is 2000rpm is added
It after revolving mixing 3min, then is filtered with the PES filter membranes of 0.45um, obtains supernatant, take above-mentioned supernatant 200uL respectively, use
High performance liquid chromatograph and DAD detectors are detected above-mentioned supernatant, obtain third in above-mentioned seven kinds of standard solution respectively
Valeric acid chromatogram and caprylic acid chromatogram, with above-mentioned seven valproic acid peak areas of standard solution and being compared to for caprylic acid peak area
For the ordinate y of canonical plotting, contained just with standard internal standard solution with the concentration containing valproic acid in above-mentioned standard working solution
Abscissa x of the ratio between the sad concentration as canonical plotting will detect seven groups of data of gained and carry out linear regression, be fitted above
It is y=a*x+b to calibration curve equation, and obtains weight coefficient a and b, standard working solution is the solution containing valproic acid, mark
Quasi- internal standard solution is the solution containing caprylic acid；
(a) preparation of standard working solution：
Accurately weighing valproic acid standard items 45mg is placed in 1ml volumetric flasks, is dissolved with methanol, and constant volume is obtained in 1ml
Standard reserving solution A, a concentration of 45000mg/L of valproic acid, the methanol solution for being 30% by standard reserving solution A water content
Dilution is diluted, the valproic acid respectively containing 400,800,1200,1600,2000,3000 and 4,000 seven kinds of concentration of μ g/mL
Solution, and preserved under the conditions of -80 DEG C；
(b) preparation of standard internal standard solution
Caprylic acid standard items 210.79mg accurately is weighed in 2mL test tubes, is dissolved with 2mL methanol, is obtained internal standard storing solution
B, a concentration of 105395mg/L of caprylic acid carry out the dilution of the internal standard storing solution B methanol solutions for being 30% with water content
Dilution, obtains the standard internal standard solution containing a concentration of 10500mg/L of caprylic acid, and preserved under the conditions of -80 DEG C,
(2) centrifugation of blood is detected
Blood to be detected at least 5ml is taken, 10min is centrifuged in the case where centrifugal speed is 3500rpm, obtains supernatant i.e. serum,
It is spare to before analyzing that above-mentioned serum is placed in the lower preservation of -20 DEG C of freezings；
(3) sample to be tested is handled
(c) it uses the standard internal standard solution 10uL of liquid-transfering gun removing step (b) in the centrifuge tube of 1.5ml, step is then added
(2) serum of 200uL centrifuges 30s-1min in above-mentioned centrifuge tube in the case where centrifugal speed is 1500-2500rpm；Then it uses
Liquid-transfering gun pipettes the 200 μ L of diluent containing 0.4-0.6% high chloro acid solutions and is added in above-mentioned centrifuge tube, then with 0.45um's
Filter membrane is filtered, and it is sample to be tested to obtain supernatant；
(4) detection of sample to be tested
Removing step (c) sample to be tested 200uL, using high performance liquid chromatograph and DAD detectors to above-mentioned sample to be tested
It is detected, the valproic acid chromatogram and caprylic acid chromatogram of above-mentioned sample to be tested is obtained, by the valproic acid in above-mentioned chromatogram
The ratio between peak area and caprylic acid peak area y are substituted into the calibration curve equation y=a*x+b of above-mentioned steps (one), by calculating
To the ratio between caprylic acid concentration in valproic acid concentration in detected sample and standard internal standard solution x, caprylic acid concentration in standard internal standard solution
It is known, the valproic acid drug concentration in blood to be detected is obtained by calculation.
Existing high performance liquid chromatograph includes：Sample introduction module, liquid phase pump module and column temperature module, the efficient liquid phase of the application
Chromatograph further includes：On-line solid phase extraction device, on-line solid phase extraction device is between sample introduction module and liquid phase pump module, online
Extraction column used in solid-phase extraction device is Thermo TurboF1ow C18XL；The extraction column of on-line solid phase extraction instrument flows
It is mutually the water containing 0.015% phosphoric acid and 10mmol/L sodium dihydrogen phosphates, extraction column flow rate is 1.5mL/min, and extraction column uses
Recoil pattern.
Analysis chromatographic column used in high performance liquid chromatograph is Agilent Extend-C18；Used on-line filtration
Device is SSI COL PRE-FILTER WATER1/16 0.5M, and the column temperature of high performance liquid chromatograph setting is 50 DEG C, and sample size is
100uL, analysis column Mobile phase contain the organic phase of 22: 78 ratios：Water phase, wherein organic phase be acetonitrile, water phase be containing
The water of 0.015% phosphoric acid and 10mmol/L sodium dihydrogen phosphates, analysis column flow rate are 1.0mL/min, and analysis chromatographic column uses
Isocratic elution mode.
DVD detectors are Vanquish diode array detector, and Detection wavelength 212nm, water content is volume ratio
Water content.
Technical method demonstration is as follows in the present embodiment：
One, the linear relationship and quantitative limit of this method
By the valproic acid standard working solution of each concentration (400-4000 μ g/mL) of 10 μ L of above-mentioned preparation, 190 μ L are added
Blank serum mixing, the sample introduction after dilution, the concentration of valproic acid are measured in 20mg/L to 200mg/L ranges by the present embodiment
Condition is measured from low to high by concentration, is mapped with quantitative chromatographic peak area-concentration, is obtained standard curve, the results showed that third
The range of linearity and quantitative limit of valeric acid are as follows：
(1) detection limit (LOD)：4.17mg/L.
(2) quantitative limit (LOQ)：13.89mg/L.
(3) range of linearity：
Valproic acid is linear good in 20mg/L to 200mg/L ranges, coefficient R2＞ 0.99.
Two, the rate of recovery and precision of this method
Taking valproic acid standard working solution to be configured to, high, medium and low 3 kinds of concentration carries out sample recovery rate experiment and precision is real
It tests, is measured by the present embodiment method, replicate analysis measures 3 batches, and the rate of recovery and precision are respectively such as table 1.Its it is low,
Average recovery rate within the scope of 3 middle and high pitch-based spheres is 98.85%~100.86%, relative standard deviation 1.23%
~1.93%, table 1 is valproic acid recovery of standard addition and precision.
| Mark-on amount/(mg/L) | 40.00 | 80.00 | 150.00 |
| Average recovery rate/% | 98.85 | 99.98 | 100.86 |
| Precision RSD/% | 1.23 | 1.73 | 1.93 |
Table 1
In summary verification test, the detection limit of the present embodiment, all technicals such as the rate of recovery and precision meet
It is required that valproic acid drug concentration in method detection blood, reproducibility is good, and sample recovery rate is high, improves the standard of testing result
Exactness.
Valproic acid chromatogram is shown in Fig. 3 in serum sample, and valproic acid chromatogram is shown in Fig. 2 in standard solution, when valproic acid retains
Between be 6.65min, internal standard retention time is 9.80min, by the identification of the present embodiment method target compound known to Fig. 2 and Fig. 3
Accurately, and analysis time is short, interferes small, high specificity.
Embodiment described above is only that the preferred embodiment of the present invention is described, not to the model of the present invention
It encloses and is defined, under the premise of not departing from design spirit of the present invention, technical side of the those of ordinary skill in the art to the present invention
The various modifications and improvement that case is made should all be fallen into the protection domain of claims of the present invention determination.
Claims (10)
1. the on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in a kind of detection blood, it is characterised in that：It is wrapped
Include following steps：
(1) calibration of standard solution
First with liquid-transfering gun by 10 μ L of standard working solution of at least three kinds various concentrations, 10 μ L standards internal standard solutions and 190 μ L blank
Serum, which is respectively placed in 1.5ml centrifuge tubes, to be mixed and made at least three kinds of standard solution, above-mentioned standard solution and is in rotating speed respectively
Under 1000-2000rpm after vortex mixing 30s-1min, the 200 μ L of diluent containing 0.4-0.6% high chloro acid solutions are added,
And be filtered after rotating speed is vortex mixing 2-4min under 1200-2000rpm, then with the PES filter membranes of 0.45um, obtain supernatant
Liquid takes above-mentioned supernatant 200uL, is detected to above-mentioned supernatant with high performance liquid chromatograph and DAD detectors respectively, respectively
The valproic acid chromatogram and caprylic acid chromatogram in above-mentioned at least three kinds of standard solution are obtained, with above-mentioned at least three standard solution
Ordinate y as canonical plotting of valproic acid peak area and the ratio between caprylic acid peak area, to contain in above-mentioned standard working solution
Have the concentration of valproic acid and the abscissa x containing the ratio between caprylic acid concentration as canonical plotting in standard internal standard solution, will more than
Detection gained at least three groups of data carry out linear regression, and it is y=a*x+b that fitting, which obtains calibration curve equation, and obtains weight
Coefficient a and b, standard working solution are the solution containing valproic acid, and standard internal standard solution is the solution containing caprylic acid；
(a) preparation of standard working solution：
Accurately weighing valproic acid standard items 45mg is placed in 1ml volumetric flasks, is dissolved with methanol, and constant volume obtains standard in 1ml
Storing solution A, a concentration of 45000mg/L of valproic acid, the methanol solution for being 10%-40% with water content by standard reserving solution A
Dilution be diluted, configured in the range of containing 400-4000 μ g/mL valproic acids respectively each concentration standard work
Liquid, and preserved under the conditions of -80 DEG C；
(b) preparation of standard internal standard solution
Caprylic acid standard items 210.79mg accurately is weighed in 2mL test tubes, is dissolved with 2mL methanol, is obtained internal standard storing solution B,
A concentration of 105395mg/L of caprylic acid carries out the dilution of the internal standard storing solution B methanol solutions for being 10%-40% with water content
Dilution, obtains the standard internal standard solution containing a concentration of 10500mg/L of caprylic acid, and preserved under the conditions of -80 DEG C,
(2) centrifugation of blood is detected
Blood to be detected at least 5ml is taken, 10min is centrifuged in the case where centrifugal speed is 3500rpm, obtains supernatant i.e. serum, it will be upper
State serum be placed in -20 DEG C of freezings it is lower preserve it is spare to before analyzing；
(3) sample to be tested is handled
(c) it uses the standard internal standard solution 10uL of liquid-transfering gun removing step (b) in the centrifuge tube of 1.5ml, step (2) is then added
200uL serum in above-mentioned centrifuge tube, centrifugal speed be 1500-2500rpm under centrifuge 30s-1min；Then liquid relief is used
Rifle pipettes the 200 μ L of diluent containing 0.4-0.6% high chloro acid solutions and is added in above-mentioned centrifuge tube, then with the filter membrane of 0.45um
It is filtered, it is sample to be tested to obtain supernatant；
(4) detection of sample to be tested
Removing step (c) sample to be tested 200uL carries out above-mentioned sample to be tested using high performance liquid chromatograph and DAD detectors
Detection, obtains the valproic acid chromatogram and caprylic acid chromatogram of above-mentioned sample to be tested, by the valproic acid peak face in above-mentioned chromatogram
The ratio between product and caprylic acid peak area y are substituted into the calibration curve equation y=a*x+b of above-mentioned steps (one), are obtained by calculation and are waited for
The ratio between valproic acid concentration and caprylic acid concentration in standard internal standard solution x in sample are detected, caprylic acid concentration is in standard internal standard solution
Know, the valproic acid drug concentration in blood to be detected is obtained by calculation.
2. the on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in blood is detected as described in claim 1,
It is characterized in that：The standard working solution of seven kinds of various concentrations, the standard working solution point of seven kinds of various concentrations are used in step (1)
The valproic acid solution of 400,800,1200,1600,2000,3000 and 4000 μ g/mL concentration Wei not contained.
3. the on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in blood is detected as claimed in claim 2,
It is characterized in that：The precipitating reagent is containing 0.5% high chloro acid solution.
4. the on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in blood is detected as claimed in claim 3,
It is characterized in that：Dilution in step (a) and (b) is by 3：The dilution of 7 water and methanol composition.
5. the on-line solid phase extraction liquid phase chromatography analytical method of Clonazepam content in blood is detected as claimed in claim 4,
It is characterized in that：The high performance liquid chromatograph further includes：On-line solid phase extraction device, used in on-line solid phase extraction device
Extraction column is Thermo TurboFlow C18 XL.
6. the on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in blood is detected as claimed in claim 5,
It is characterized in that：Analysis chromatographic column used in the high performance liquid chromatograph is Agilent Extend-C18；It is used
Line filter is 1/16 0.5M of SSI COL PRE-FILTER WATER, and the column temperature of high performance liquid chromatograph setting is 50 DEG C,
Sample size is 100uL.
7. the on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in blood is detected as claimed in claim 5,
It is characterized in that：The extraction column mobile phase of the on-line solid phase extraction instrument is to contain 0.015% phosphoric acid and 10mmol/L biphosphates
The water of sodium, extraction column flow rate are 1.5mL/min, and extraction column uses recoil pattern.
8. the on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in blood is detected as claimed in claim 6,
It is characterized in that：The analysis column Mobile phase contains 22：The organic phase of 78 ratios：Water phase, wherein organic phase are acetonitrile, water phase
For the water containing 0.015% phosphoric acid and 10mmol/L sodium dihydrogen phosphates, analysis column flow rate is 1.0mL/min, analyzes chromatographic column
Using isocratic elution mode.
9. the on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in blood is detected as claimed in claim 7 or 8,
It is characterized in that：The DVD detectors are Vanquish diode array detector, Detection wavelength 212nm.
10. the on-line solid phase extraction liquid phase chromatography analytical method of content of valproic acid in blood is detected as claimed in claim 9,
It is characterized in that：The water content is the water content of volume ratio.
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| CN114814005A (en) * | 2022-04-01 | 2022-07-29 | 北京和合医学诊断技术股份有限公司 | Liquid chromatography analysis method for detecting ziprasidone in blood |
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