CN109115921A - A kind of method that A Pa replaces Buddhist nun's drug concentration in measurement human plasma - Google Patents
A kind of method that A Pa replaces Buddhist nun's drug concentration in measurement human plasma Download PDFInfo
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Abstract
The present invention relates to the methods that A Pa in the clinical blood concentration detection technique field of anti-tumor drug more particularly to a kind of measurement human plasma replaces Buddhist nun's drug concentration.Buddhist nun's drug concentration, pre-treatment and high performance liquid chromatography tandem mass spectrum (HPLC-MS/MS) analytical procedure including blood sample are replaced using A Pa in HPLC-MS/MS method measurement human plasma.A Pa in blood sample to be measured is quantified for Buddhist nun's drug concentration by internal standard method.The present invention combines internal standard method with high performance liquid chromatography tandem mass spectrometry, improve the accuracy of quantitative result, eliminate systematic error, shorten analysis time, keep detection process easy quickly, it is detected conducive to the Ah pa in clinical treatment to patient's body for Buddhist nun's blood concentration, provides experiment basis for Ah pa for Personalized Drug Administration, the reduction toxicity of Buddhist nun.
Description
Technical field
The present invention relates to the clinical blood concentration detection technique field of anti-tumor drug more particularly to a kind of measurement human plasmas
The method that middle A Pa replaces Buddhist nun's drug concentration.
Background technique
A Pa presses down for the small molecule tyrosine kinase that Buddhist nun is a kind of intracellular APT binding site of eight VEGFR2 of new oral
Preparation has preferable therapeutic effect and safety to gastric cancer, is clinically mainly used for advanced stage sdenocarcinoma of stomach or stomach-test tube engaging portion gland
Treatment more than three line of cancer or three lines.Since there are biggish individual difference, internal blood concentration and clinics for internal metabolic process
Curative effect is closely related with adverse reaction, detects its blood concentration so as to adjust dosage and number, reduces adverse reaction, facing
It is of great significance in bed medication.
Ah pa is rarely reported for the measuring method of blood concentration in Buddhist nun's body at present, without effective intra-body data index
Clinical application is instructed, clinician mostly uses Observations Means to judge curative effect.The internal blood medicine of domestic most tumors drug is dense
The measurement of degree mostly uses liquid chromatography and liquid phase tandem mass spectrometry, the former is long analysis time, consumes a large amount of organic solvents, minimum
The problems such as lower limit of quantitation is larger.
Summary of the invention
In order to solve the above technical problems, the present invention provide it is a kind of measurement human plasma in A Pa replace Buddhist nun's drug concentration method,
Using HPLC-MS/MS method, internal standard method is combined with high performance liquid chromatography tandem mass spectrometry, establishes a kind of quick, sensitive survey
Determine the method that A Pa replaces Buddhist nun's concentration in human plasma.
Specific technical solution is as follows:
A kind of method that A Pa replaces Buddhist nun's drug concentration in measurement human plasma, is examined using high performance liquid chromatography tandem mass spectrum
It surveys comprising the steps of:
(1) preparation of standard working solution
It accurately weighs Ah pa to be placed in 1.5mL centrifuge tube for Buddhist nun's standard items 1.02mg, the accurate addition pure methanol of 1.02mL is molten
Liquid is dissolved, and obtains standard reserving solution A, i.e., the Ah pa that concentration is 1mg/mL replaces Buddhist nun's solution, by the pure methanol of standard reserving solution A
Solution is diluted, and is diluted to the Ah pa of 0.04-40ug/mL concentration for Buddhist nun's solution, and save under the conditions of -80 DEG C；
(2) preparation of internal standard working solution
It accurately weighs Benazeprilat standard items 1.0mg to be placed in 1.5mL centrifuge tube, the accurate addition pure methanol of 1.0mL is molten
Liquid is dissolved, and standard reserving solution B is obtained, and standard reserving solution B is diluted with pure methanol, and obtaining concentration is the interior of 5ug/mL
Working solution is marked, and is saved under the conditions of -80 DEG C；
(3) centrifugation of blood is detected
Blood to be detected at least 2mL is taken, 10min is centrifuged in the case where centrifugal speed is 3500rpm, supernatant is taken to obtain blood plasma, on
State blood plasma be placed in -20 DEG C of freezings it is lower save it is spare to before analyzing；
(4) sample to be tested is handled
(1) internal standard working solution described in 10 μ L step (2) is pipetted in 1.5mL centrifuge tube with liquid-transfering gun, 50 μ L step is added
Suddenly blood plasma described in (three), be vortexed concussion mixing 1min under the revolving speed of 1500rpm；
(2) it is added in the centrifuge tube of step (1) with the methanol solution that liquid-transfering gun pipettes 200 μ L formic acid containing 0.1-0.3%,
Be vortexed concussion mixing 2min under the revolving speed of 1500rpm, and high speed centrifugation 5min, obtains supernatant under the revolving speed of 14000rpm；
(3) in the 20 μ L of supernatant and 1.5mL centrifuge tube in liquid-transfering gun removing step (2), 380 μ L is added and redissolve liquid,
Be vortexed concussion mixing 2min under the revolving speed of 2000rpm, and obtained solution is sample to be tested；
(5) measurement of sample to be tested
(4) preparation of standard curve
The 50 μ L of standard working solution that (one) plants eight kinds of various concentrations is mixed with 950 μ L normal person's blank plasmas respectively first
Standard work blood medicine is made, is handled according to the step in (four), obtained mark song sample is detected into instrument, is obtained
The Ah pa of nine kinds of standard solution is stated for Buddhist nun and internal standard chromatogram, standard target object peak area and internal standard are respectively obtained in chromatogram
Object peak area, using the ratio of standard target object peak area and internal standard compound peak area as the ordinate y of canonical plotting, with standard
Working solution prepare after in blood plasma ratio, that is, relative concentration of target to be measured blood concentration and internal standard working solution concentration as standard
The above detection data is carried out linear regression by weighted least-squares method, obtains calibration curve equation by the abscissa x of curve graph
For y=a × x+b, and obtain weight coefficient a and b.
(5) measurement of sample to be tested
Sample to be measured in above-mentioned steps (3) is monitored using high performance liquid chromatography tandem mass spectrum analysis instrument, is obtained
The Ah pa of above-mentioned sample to be tested replaces Buddhist nun and internal standard chromatogram out, replaces in Buddhist nun and internal standard chromatogram in above-mentioned Ah pa and obtains object to be measured
Object peak area and internal standard peak area are brought into and calculate the concentration that Ah pa replaces Buddhist nun in the calibration curve equation in (4).
(6) HPLC-MS/MS condition
Chromatographic condition: 3.5 μm of ZORBAX Eclipse Plus C18,4.6 × 100mm chromatographic column are used；Column temperature is 35
℃.Mobile phase A is mutually methanol, and B phase is the water containing 0.1% formic acid with 65:35 ratio progress isocratic elution.Flow velocity 0.5mL/min,
Sample volume 5 μ L, analysis time 3.8min.
Mass Spectrometry Conditions: electric spray ion source is used, in positive ion electrospray from the mass spectrum under mode, selecting more reaction detection modes
Scanning mode is measured, and Ah pa is respectively 398.2,397.18 for Buddhist nun and internal standard Benazeprilat parent ion, daughter ion difference
It is 212,351.1, retention time is respectively 3.2,2.6min.
A Pa contained in the quasi- working solution of standard working solution concentration index replaces the concentration of Buddhist nun, and internal standard working solution concentration refers to
The concentration of Benazeprilat contained in internal standard working solution.
The standard working solution of nine kinds of various concentrations is used in step (1).
The standard working solution of nine kinds of various concentrations contains 0.04 respectively, 0.1,0.2,0.5,1.0,4.0,10.0,20.0,
The Ah pa of 40.0ug/mL concentration replaces Buddhist nun's solution.
Redissolution liquid used in step (3) is the water that 65% methanol -35% contains 0.1% formic acid.
Compared with prior art, the present invention has following advantageous effects:
Liquid chromatography tandem mass spectrometry measures that blood concentration high sensitivity, specificity be strong, favorable reproducibility；High degree it is short
Analysis time reduces the dosage of organic solvent and then reduces the pollution to environment；Reduce minimum lower limit of quantitation.
Detailed description of the invention
Fig. 1 replaces the typical chromatographic peak of Buddhist nun and Benazeprilat for A Pa after 3 sample process of embodiment.
Specific embodiment
The present invention is described in detail with specific embodiment below in conjunction with the accompanying drawings, but protection scope of the present invention is not by attached
Figure and embodiment are limited.
Embodiment 1
A kind of method that A Pa replaces Buddhist nun's drug concentration in measurement human plasma, is examined using high performance liquid chromatography tandem mass spectrum
It surveys comprising the steps of:
(1) preparation of standard working solution
It accurately weighs Ah pa to be placed in 1.5mL centrifuge tube for Buddhist nun's standard items 1.02mg, the accurate addition pure methanol of 1.02mL is molten
Liquid is dissolved, and obtains standard reserving solution A, i.e., the Ah pa that concentration is 1mg/mL replaces Buddhist nun's solution, by the pure methanol of standard reserving solution A
Solution is diluted, be diluted to 0.04,0.1,0.2,0.5,1.0,4.0,10.0,20.0, the Ah pa of 40.0ug/mL concentration is for Buddhist nun
Solution, and saved under the conditions of -80 DEG C；
(2) preparation of internal standard working solution
It accurately weighs Benazeprilat standard items 1.0mg to be placed in 1.5mL centrifuge tube, the accurate addition pure methanol of 1.0mL is molten
Liquid is dissolved, and standard reserving solution B is obtained, and standard reserving solution B is diluted with pure methanol, and obtaining concentration is the interior of 5ug/mL
Working solution is marked, and is saved under the conditions of -80 DEG C；
(3) centrifugation of blood is detected
Blood 3mL to be detected is taken, 10min is centrifuged in the case where centrifugal speed is 3500rpm, supernatant is taken to obtain blood plasma, above-mentioned blood
It is spare to before analyzing that slurry is placed in the lower preservation of -20 DEG C of freezings；
(4) sample to be tested is handled
(1) internal standard working solution described in 10 μ L step (2) is pipetted in 1.5mL centrifuge tube with liquid-transfering gun, 50 μ L step is added
Suddenly blood plasma described in (three), be vortexed concussion mixing 1min under the revolving speed of 1500rpm；
(2) methanol solution of the 200 μ L containing 0.1% formic acid is pipetted with liquid-transfering gun to be added in the centrifuge tube of step (1),
Be vortexed concussion mixing 2min under the revolving speed of 1500rpm, and high speed centrifugation 5min, obtains supernatant under the revolving speed of 14000rpm；
(3) in the 20 μ L of supernatant and 1.5mL centrifuge tube in liquid-transfering gun removing step (2), 380 μ L is added and redissolve liquid,
Be vortexed concussion mixing 2min under the revolving speed of 2000rpm, and obtained solution is sample to be tested；
Embodiment 2
(1) preparation of standard working solution
It accurately weighs Ah pa to be placed in 1.5mL centrifuge tube for Buddhist nun's standard items 1.02mg, the accurate addition pure methanol of 1.02mL is molten
Liquid is dissolved, and obtains standard reserving solution A, i.e., the Ah pa that concentration is 1mg/mL replaces Buddhist nun's solution, by the pure methanol of standard reserving solution A
Solution is diluted, be diluted to 0.04,0.1,0.2,0.5,1.0,4.0,10.0,20.0, the Ah pa of 40.0ug/mL concentration is for Buddhist nun
Solution, and saved under the conditions of -80 DEG C；
(2) preparation of internal standard working solution
It accurately weighs Benazeprilat standard items 1.0mg to be placed in 1.5mL centrifuge tube, the accurate addition pure methanol of 1.0mL is molten
Liquid is dissolved, and standard reserving solution B is obtained, and standard reserving solution B is diluted with pure methanol, and obtaining concentration is the interior of 5ug/mL
Working solution is marked, and is saved under the conditions of -80 DEG C；
(3) centrifugation of blood is detected
Blood 3mL to be detected is taken, 10min is centrifuged in the case where centrifugal speed is 3500rpm, supernatant is taken to obtain blood plasma, above-mentioned blood
It is spare to before analyzing that slurry is placed in the lower preservation of -20 DEG C of freezings；
(4) sample to be tested is handled
(1) internal standard working solution described in 10 μ L step (2) is pipetted in 1.5mL centrifuge tube with liquid-transfering gun, 50 μ L step is added
Suddenly blood plasma described in (three), be vortexed concussion mixing 1min under the revolving speed of 1500rpm；
(2) methanol solution of the 200 μ L containing 0.3% formic acid is pipetted with liquid-transfering gun to be added in the centrifuge tube of step (1),
Be vortexed concussion mixing 2min under the revolving speed of 1500rpm, and high speed centrifugation 5min, obtains supernatant under the revolving speed of 14000rpm；
(3) in the 20 μ L of supernatant and 1.5mL centrifuge tube in liquid-transfering gun removing step (2), 380 μ L is added and redissolve liquid,
Be vortexed concussion mixing 2min under the revolving speed of 2000rpm, and obtained solution is sample to be tested；
Embodiment 3
(1) preparation of standard working solution
It accurately weighs Ah pa to be placed in 1.5mL centrifuge tube for Buddhist nun's standard items 1.02mg, the accurate addition pure methanol of 1.02mL is molten
Liquid is dissolved, and obtains standard reserving solution A, i.e., the Ah pa that concentration is 1mg/mL replaces Buddhist nun's solution, by the pure methanol of standard reserving solution A
Solution is diluted, be diluted to 0.04,0.1,0.2,0.5,1.0,4.0,10.0,20.0, the Ah pa of 40.0ug/mL concentration is for Buddhist nun
Solution, and saved under the conditions of -80 DEG C；
(2) preparation of internal standard working solution
It accurately weighs Benazeprilat standard items 1.0mg to be placed in 1.5mL centrifuge tube, the accurate addition pure methanol of 1.0mL is molten
Liquid is dissolved, and standard reserving solution B is obtained, and standard reserving solution B is diluted with pure methanol, and obtaining concentration is the interior of 5ug/mL
Working solution is marked, and is saved under the conditions of -80 DEG C；
(3) centrifugation of blood is detected
Blood 2mL to be detected is taken, 10min is centrifuged in the case where centrifugal speed is 3500rpm, supernatant is taken to obtain blood plasma, above-mentioned blood
It is spare to before analyzing that slurry is placed in the lower preservation of -20 DEG C of freezings；
(4) sample to be tested is handled
(1) internal standard working solution described in 10 μ L step (2) is pipetted in 1.5mL centrifuge tube with liquid-transfering gun, 50 μ L step is added
Suddenly blood plasma described in (three), be vortexed concussion mixing 1min under the revolving speed of 1500rpm；
(2) methanol solution of the 200 μ L containing 0.2% formic acid is pipetted with liquid-transfering gun to be added in the centrifuge tube of step (1),
Be vortexed concussion mixing 2min under the revolving speed of 1500rpm, and high speed centrifugation 5min, obtains supernatant under the revolving speed of 14000rpm；
(3) in the 20 μ L of supernatant and 1.5mL centrifuge tube in liquid-transfering gun removing step (2), 380 μ L is added and redissolve liquid,
Be vortexed concussion mixing 2min under the revolving speed of 2000rpm, and obtained solution is sample to be tested；
(5) measurement of sample to be tested
(4) preparation of standard curve
First by the 50 μ L of standard working solution of eight kinds of various concentrations in step (1) respectively with 950 μ L normal person's blank plasmas
It is mixed and made into standard work blood medicine, is handled according to the step in (four), obtained mark song sample is detected into instrument, is obtained
Out the Ah pa of above-mentioned nine kinds of standard solution replace Buddhist nun and internal standard chromatogram, respectively obtained in chromatogram standard target object peak area and
Internal standard compound peak area, using the ratio of standard target object peak area and internal standard compound peak area as the ordinate y of canonical plotting, with
Standard working solution prepare after in blood plasma target to be measured blood concentration and internal standard working solution concentration ratio, that is, relative concentration conduct
The abscissa x of canonical plotting, linear relationship related data are shown in Table 1, by the above detection data by weighted least-squares method into
Row linear regression, obtaining calibration curve equation is y=0.270 × x-0.0026.
1 linear relationship related data of table
| Sample introduction title | Determinand peak area | Internal standard peak area | Y value | Determinand blood concentration | Internal standard concentration | X value |
| sd1_01 | 267 | 2204 | 0.1211 | 40 | 5.0×103 | 0.008 |
| sd2_01 | 692 | 2474 | 0.2797 | 100 | 5.0×103 | 0.020 |
| sd3_01 | 1262 | 2242 | 0.5629 | 200 | 5.0×103 | 0.040 |
| sd4_01 | 2950 | 2292 | 1.2871 | 500 | 5.0×103 | 0.100 |
| sd5_01 | 6199 | 2201 | 2.8164 | 1.0×103 | 5.0×103 | 0.200 |
| sd6_01 | 24022 | 2275 | 10.5591 | 4.0×103 | 5.0×103 | 0.800 |
| sd7_01 | 60749 | 2180 | 27.8665 | 10.0×103 | 5.0×103 | 2.000 |
| Sd8_01 | 129909 | 2275 | 57.1029 | 20.0×103 | 5.0×103 | 4.000 |
| Sd9_01 | 265963 | 2285 | 116.3952 | 40.0×103 | 5.0×103 | 8.000 |
(5) measurement of sample to be tested
Sample to be measured in above-mentioned steps (3) is monitored using high performance liquid chromatography tandem mass spectrum analysis instrument, is obtained
The Ah pa of above-mentioned sample to be tested replaces Buddhist nun and internal standard chromatogram out, replaces in Buddhist nun and internal standard chromatogram in above-mentioned Ah pa and obtains object to be measured
The ratio y of object peak area and internal standard peak area is updated in the calibration curve equation in (4), and thunder in determinand is obtained by calculation
For the relative concentration x of Qu Sai, internal standard compound working solution concentration be it is known, the drug of Raltitrexed in determinand is thus calculated
Concentration.
(6) HPLC-MS/MS condition
Chromatographic condition: 3.5 μm of ZORBAX Eclipse Plus C18,4.6 × 100mm chromatographic column are used；Column temperature is 35
℃.Mobile phase A is mutually methanol, and B phase is the water containing 0.1% formic acid with 65:35 ratio progress isocratic elution.Flow velocity 0.5mL/min,
Sample volume 5 μ L, analysis time 3.8min.
Mass Spectrometry Conditions: electric spray ion source is used, in positive ion electrospray from the mass spectrum under mode, selecting more reaction detection modes
Scanning mode is measured, and Ah pa is respectively that 398.2,397.18 daughter ions are respectively with internal standard Benazeprilat parent ion for Buddhist nun
212,351.1, retention time is respectively 3.2,2.6min, and Fig. 1 is that A Pa is replaced after minimum lower limit of quantitation sample process in embodiment 3
The typical chromatographic peak of Buddhist nun and Benazeprilat.
Methodology validation
The linear relationship and quantitative limit of this method: standard curve is obtained by method in above-mentioned (4), the results showed that Ah pa replaces Buddhist nun
The range of linearity and quantitative limit it is as follows: (1) quantitative limit (LOQ): 2ng/mL；(2) range of linearity: Ah pa arrives for Buddhist nun in 2ng/mL
It is linear good within the scope of 2000ng/mL, coefficient R 2 > 0.99.
This method rate of recovery and precision: it takes Ah pa to be configured to high, medium and low 3 concentration for Buddhist nun's stock solution and carries out the rate of recovery
And Precision Experiment, pre-processed by embodiment 3, be measured by embodiment, 3 batches of replication, the rate of recovery and
Precision such as its average recovery rate in high, medium and low 3 concentration levels of following table 1. are 103.29%~109.24%, relatively
Standard deviation is 1.25%~4.99%.
1 Ah pa of table replaces Buddhist nun's rate of recovery and precision
| Solution concentration | 4ng/mL | 100ng/mL | 1600ng/mL |
| Average recovery rate | 109.24 | 108.00 | 103.29 |
| Precision RSD | 4.99 | 1.25 | 4.75 |
In summary confirmatory experiment, all technicals such as the rate of recovery and precision of this method meet the requirements, method
It detects A Pa in blood and replaces Buddhist nun's drug concentration, reproducibility is good, and the rate of recovery is high, and detection accuracy is high.
This method is quantified using internal standard method, eliminates systematic error, is that the analysis of target compound is more accurate, and
And analysis time is short, it is small to interfere.
Claims (5)
1. a kind of method that A Pa replaces Buddhist nun's drug concentration in measurement human plasma, is examined using high performance liquid chromatography tandem mass spectrum
It surveys, which is characterized in that comprise the steps of:
(1) preparation of standard working solution
Accurately Ah pa is weighed to be placed in 1.5mL centrifuge tube for Buddhist nun's standard items 1.02mg, it is accurate be added the pure methanol solution of 1.02mL into
Row dissolution obtains standard reserving solution A, i.e., the Ah pa that concentration is 1mg/mL replaces Buddhist nun's solution, by the pure methanol solution of standard reserving solution A
It is diluted, is diluted to the Ah pa of 0.04-40ug/mL concentration for Buddhist nun's solution, and save under the conditions of -80 DEG C；
(2) preparation of internal standard working solution
Benazeprilat standard items 1.0mg is accurately weighed to be placed in 1.5mL centrifuge tube, it is accurate be added the pure methanol solution of 1.0mL into
Row dissolution, obtains standard reserving solution B, standard reserving solution B is diluted with pure methanol, obtains the internal standard work that concentration is 5ug/mL
Make liquid, and is saved under the conditions of -80 DEG C；
(3) centrifugation of blood is detected
Blood to be detected at least 2mL is taken, 10min is centrifuged in the case where centrifugal speed is 3500rpm, supernatant is taken to obtain blood plasma, above-mentioned blood
It is spare to before analyzing that slurry is placed in the lower preservation of -20 DEG C of freezings；
(4) sample to be tested is handled
(1) internal standard working solution described in 10 μ L step (2) is pipetted in 1.5mL centrifuge tube with liquid-transfering gun, 50 μ L steps are added
(3) blood plasma described in, be vortexed concussion mixing 1min under the revolving speed of 1500rpm；
(2) it is added in the centrifuge tube of step (1) with the methanol solution that liquid-transfering gun pipettes 200 μ L formic acid containing 0.1-0.3%,
Be vortexed concussion mixing 2min under the revolving speed of 1500rpm, and high speed centrifugation 5min, obtains supernatant under the revolving speed of 14000rpm；
(3) in the 20 μ L of supernatant and 1.5mL centrifuge tube in liquid-transfering gun removing step (2), 380 μ L is added and redissolve liquid,
Be vortexed concussion mixing 2min under the revolving speed of 2000rpm, and obtained solution is sample to be tested；
(5) measurement of sample to be tested
(4) preparation of standard curve
The 50 μ L of standard working solution that (one) plants eight kinds of various concentrations is mixed with 950 μ L normal person's blank plasmas respectively first
Standard work blood medicine, is handled, obtained mark song sample is detected into instrument, obtains above-mentioned nine according to the step in (four)
The Ah pa of kind standard solution replaces Buddhist nun and internal standard chromatogram, and standard target object peak area and internal standard compound peak are respectively obtained in chromatogram
Area is worked using the ratio of standard target object peak area and internal standard compound peak area as the ordinate y of canonical plotting with standard
Liquid prepare after in blood plasma ratio, that is, relative concentration of target to be measured blood concentration and internal standard working solution concentration as standard curve
The above detection data is carried out linear regression by weighted least-squares method by the abscissa x of figure, and obtaining calibration curve equation is y
=a × x+b, and obtain weight coefficient a and b.
(5) measurement of sample to be tested
Sample to be measured in above-mentioned steps (3) is monitored using high performance liquid chromatography tandem mass spectrum analysis instrument, is obtained
The Ah pa of sample to be tested is stated for Buddhist nun and internal standard chromatogram, is replaced in Buddhist nun and internal standard chromatogram in above-mentioned Ah pa and obtains target to be measured peak
Area and internal standard peak area are brought into and calculate the concentration that Ah pa replaces Buddhist nun in the calibration curve equation in (4).
(6) HPLC-MS/MS condition
Chromatographic condition: 3.5 μm of ZORBAX Eclipse Plus C18,4.6 × 100mm chromatographic column are used；Column temperature is 35 DEG C.Stream
Dynamic phase A phase is methanol, and B phase is the water containing 0.1% formic acid with 65:35 ratio progress isocratic elution.Flow velocity 0.5mL/min, sample introduction
Measure 5 μ L, analysis time 3.8min.
Mass Spectrometry Conditions: electric spray ion source is used, in positive ion electrospray from the scanning of the mass spectrum under mode, selecting more reaction detection modes
Mode is measured, and Ah pa is respectively 398.2,397.18 for Buddhist nun and internal standard Benazeprilat parent ion, and daughter ion is respectively
212,351.1, retention time is respectively 3.2,2.6min.
2. the method that A Pa replaces Buddhist nun's drug concentration in measurement human plasma as described in claim 1, it is characterised in that: the standard
A Pa contained in the quasi- working solution of working solution concentration index replaces the concentration of Buddhist nun, and internal standard working solution concentration refers to contained in internal standard working solution
There is the concentration of Benazeprilat.
3. the method that A Pa replaces Buddhist nun's drug concentration in measurement human plasma as claimed in claim 2, it is characterised in that: step (1)
The middle standard working solution using nine kinds of various concentrations.
4. the method that A Pa replaces Buddhist nun's drug concentration in measurement human plasma as claimed in claim 3, it is characterised in that: nine kinds of differences
The standard working solution of concentration respectively containing 0.04,0.1,0.2,0.5,1.0,4.0,10.0,20.0,40.0ug/mL concentration Ah
Pa replaces Buddhist nun's solution.
5. the method that A Pa replaces Buddhist nun's drug concentration in measurement human plasma as described in claim 1, it is characterised in that: step (3)
Used in redissolution liquid be 65% methanol -35% contain 0.1% formic acid water.
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| CN110927297A (en) * | 2019-12-25 | 2020-03-27 | 山东英盛生物技术有限公司 | Method for simultaneously detecting multiple anti-tumor drugs in blood sample |
| CN111551649A (en) * | 2020-06-08 | 2020-08-18 | 南京品生医疗科技有限公司 | Kit for detecting concentration of avibactam and ceftazidime in blood plasma and application thereof |
| CN111638285A (en) * | 2020-06-08 | 2020-09-08 | 南京品生医学检验实验室有限公司 | Method for detecting concentration of abamectin and ceftazidime in blood plasma |
| CN111879875A (en) * | 2020-08-06 | 2020-11-03 | 武汉伯瑞恒医药科技有限公司 | Method for determining cytarabine and uridine arabinoside in blood plasma |
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