CN108570474A - Rice flower development gene EH1 and its application - Google Patents
Rice flower development gene EH1 and its application Download PDFInfo
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Abstract
The invention belongs to plant genetic engineering fields, and in particular to a kind of separation clone of control rice flower development gene EH1, functional verification, and its application in paddy pollen abortion.That is, the invention discloses a kind of rice flower development gene EH1, the nucleotide sequence such as SEQ ID NO of the flower development gene EH1 of rice：Shown in 1.The present invention goes back while providing applications of the above-mentioned rice flower development gene EH1 in rice breeding, for improving rice yield.
Description
Technical field
The invention belongs to plant genetic engineering fields, and in particular to a kind of separation gram of control rice flower development gene EH1
Grand, functional verification, and its application in paddy pollen abortion.
Background technology
Rice (Oryza sativa L.) one of most important cereal crops and unifacial leaf model plant in the world, to it
The research of flower/fringe development is of great significance.It is lost by understanding molecule of rice flower/fringe during entire reproductive development in depth
Biography mechanism, on the one hand, theoretical foundation can be provided to improve the yield and quality of the gramineous crops such as rice；On the other hand,
Abundant people develop entire flowering plant/fringe to the understanding of molecular regulation mechanism.
Rice Floral development breaks up 3 stages by branch stalk differentiation spikelet differentiation and floral organ：It is mitogenetic in inflorescence first
The lower induced synthesis inflorescence meristem of tissuespecific genes activation, is differentiated to form primary branch Secondary branches and tertiary branching, after
And floral meristem bud is formed on the top of branch, it is finally differentiated to form floral organ former base again, carries out the differentiation of floral organ, by
Gradually it is differentiated to form the basic structure of the mode and flower adnation position decision spike of rice of lemma glumelle lodicule stamen gynoecium inflorescence branch.Flower
Development carry out plant first to from extraneous and the signal of itself generates reaction in vivo by certain step, from nutrient growth to life
Reproductive growth is converted to form inflorescence meristem, this process is controlled controlling for i.e. inflorescence meristem specific gene by floral genes
System.Such as dilute fringe LAX (Laxpanicle) mutant of rice[1]Show as inflorescence meristem be only differentiated to form Primary branch or
Secondary branch can be differentiated to form but do not form small ear.The key gene PLAI that control rice changes from nutrient growth to reproductive growth
Gene, mutation shows as inhibiting being differentiated to form for branch stalk (cob), to delay the beginning of reproductive growth.In floral meristem
Under the action of characteristic gene, floral meristem forms control rice inflorescence and floral meristem shape around inflorescence meristem
At the FZP genes (Frizzy panicle) of one of oligogene[2], cob curling mutant show as inflorescence meristem
The formation of indeterminate growth, floral meristem is suppressed, and small ear cannot be differentiated to form on branch stalk.Regulate and control in floral organ identity genes
Under, floral meristem is differentiated to form floral organ.At present the development model of plant flower organ from initial ABC model developments to
ABCDE, and be also continuously replenished and perfect.The function of each genoid is mainly in ABCDE models[3]：A genoids have single
Solely determine that sepal development can influence the function of petal formation again；The function of B genoids can mainly be codetermined with A genoids
The development of petal, and the development of stamen can be codetermined with C genoids；C genoids have codetermines stamen development but with B classes
The function of Carpel Development can be individually determined again；D genoids can control the development of ovule；E class functional genes are distributed in entire flower
Organ is unable to the development of some floral organ of Independent Decisiveness, but has expression in the growth course of entire floral organ.Rice
5 class functional genes of ABCDE are mainly：With the class SOUA genes for determining floral organ separate living tissue function, relative to A class functions
Gene has OsMADS14, OsMADS15, OsMADS18 and OsMADS20；Have B functional genes OsMADS2, OsMADS4 and
OsMADS16 (SUPERWOMAN1), although the two B genoids of OsMADS2, OsMADS4 have the function of it is different, in a word
Carry out effect of the B genoids in arabidopsis and rice the two species or conservative；C genoids include OsMADS3,
OsMADS58, OsMADS13 and OsMADS21, function of this 4 genes with the C genes similar to class AG, but the first two
Gene is variant on expression time, and latter two gene have the function of in different floral whorls it is different；D genoids include
OsMADS13 and OsMADS21, the two genes have non-specialization, can be expressed in ovule seed；E genoids have
OsMADS1, OsMADS5, OsMADS7, OsMADS8 and OsMADS34, wherein OsMADS1 have specially changes flower in early development stage
Allelotaxis and determine flower-shape at function, can cause floral meristem and development of floral organs not when lacking the functional gene
Completely, OsMADS5 is not very big to the effect of flower development, and OsMADS7, OsMADS8 and OsMADS34 mutation will influence the hair of flower
It educates, this 5 class SEP gene in rice is all that ABC genoids realize that normal function is essential during development of floral organs.
What is cloned at present includes LAX, FZP, OsCKX2, FON1 etc. with rice flower development related gene.LAX encodes one
A distinctive bHLH transcription factors of plant, the on the ground borderline region between portion's apical meristem and the separate living tissue newly formed
Expression is the main regulatory factors for controlling rice auxiliary primodia and being formed[4].FZP codings include an EREBP/AP2 structural domain
Transcription factor has the function of that axillary bud separate living tissue is prevented to form and establish floral meristem, and passes through regulation and control OsMADS box bases
Because of expression, floral organ characteristic is determined[5].OsCKX2 encodes a kind of enzyme of degradation of cell mitogen, and the decrease of the gene expression makes carefully
Born of the same parents' mitogen is accumulated in inflorescence meristem, increases the number of organ of multiplication, and grain number per spike is more, the final production for improving rice
Amount[6].FON1 encodes the full asphalt mixture receptor kinase of one and arabidopsis CLAVATA1 orthologs, and FON1 mutation cause
Floral meristem increases, and all floral organ numbers is caused to increase, and the wheel organ all dystopys often taken turns organ and be additionally formed are sent out
New floral organ is brought out, lubrication groove floral organ is more serious than foreign steamer organ by being influenced.Many carpellary primordia continued developments, while base
Originally still there are undifferentiated separate living tissue, but nutrient growth separate living tissue and inflorescence meristem portion big absolutely in the center for the flower reached maturity
Divide normal[7]。
Rice (Oryza sativa) is the basic grain that global nearly half population is depended on for existence, has prodigious kind in China
Area is planted, the albumen of control rice flower development gene EH1 and its coding that the present invention clones have weight in improving pollen abortion
The production application value wanted.
Involved bibliography is as follows：
1. the finely positioning science of king's Yun, Xiao Han, Qian Qian, the genetic analysis and gene that wait the dilute fringe mutant of rice are logical
Report, 2003,48:1666-1670；
2. Liu Huaqing, Wu behaves, and Duan Yuanlin waits the map based cloning Acta Genetica Sinicas of rice small ear characterizing genes FZP,
2003,30:811-816；
3.Theissen G.Development of floral organ indentity:stories from the
MADS house.Curr Opin Plant Biol,2001,4:75-85 (the development of Theissen G. floral organs：MADS families
The story phytobiology present age be in progress, 2001,4:75-85)；
4.Komatsu K,Maekawa M,Ujiie S,et al.LAX and SPA:major regulators of
shoot branching in rice.Proc Natl Acad Sci U S A,2003,100:11765-11770(Komatsu
K, Maekawa M, Ujiie S, wait .LAX and SPA：The gene National Academy of Sciences proceedings of major regulatory branch in rice,
2003,100:11765-11770)；
5.Komatsu M,Chujo A,Nagato Y,et al.FRIZZY PANICLE is required to
prevent the formation of axillary meristems and to establish floral meristem
identity in rice spikelets.Development,2003,130:3841-3850(Komatsu M,Chujo A,
Nagato Y wait .FRIZZY PANICLE to have in rice Spikelet development and prevent axillary bud separate living tissue from being formed and establish flower point
The effect Developmental Biology of raw tissue, 2003,130:3841-3850)；
6.Ashikari M,Sakakibara H,Lin S,Yamamoto T,et al.Cytokinin oxidase
regulates rice grain production.Science,2005,309:741-745(Ashikari M,
Sakakibara H, Lin S, Yamamoto T, wait cytokinin oxidases adjust rice yield science, 2005,309:
741-745)；
7.Suzaki T,Sato M,Ashikari M,et al.The gene FLORAL ORGAN
NUMBER1regulates floral meristem size in rice and encodes a leucine-rich
repeat receptor kinase orthologous to Arabidopsis CLAVATA1.Development,2004,
131:(Suzaki T, Sato M, Ashikari M wait gene FLORAL ORGAN NUMBER1 adjusting and controlling rices to 5649-5657
The repetition receptor kinase of floral meristem size, encoding leucine rich directly develops biology with arabidopsis CLAVATA1 to homologous
It learns, 2004,131:5649-5657).
Invention content
The technical problem to be solved in the present invention is to provide a kind of rice flower development gene EH1 and its answering in rice breeding
With.
To solve the above-mentioned problems, the present invention provide a kind of rice flower development gene EH1 (that is, rice it is additional glume it is prominent
Variant eh1 genes), the nucleotide sequence of the flower development gene EH1 of rice (that is, rice additional glume mutant eh1 genes)
Such as SEQ ID NO：Shown in 1.
The present invention goes back while providing applications of the above-mentioned rice flower development gene EH1 in rice breeding.
The improvement of application as the present invention：For improving rice yield.
The nucleotides sequence of rice flower development gene EH1 (that is, rice additional glume mutant eh1 genes) is classified as SEQ ID
NO：1, the amino acid sequence that the additional glume mutant of the rice corresponds to wild type is SEQ ID NO：2.
The present invention also provides the plasmids containing said gene, and the engineering bacteria containing the gene or the carrier or
Host cell.
The engineering bacteria and host cell can be regarded as engineering of the those skilled in the art used in transgenic protocol
Bacterium or host cell.But with development in science and technology, perhaps the selection of the engineering bacteria and host cell can change, or at non-turn
The application field of gene purpose is similarly related to the utilization of carrier and engineering bacteria, but as long as containing gene of the present invention or originally
The invention carrier, within protection scope of the present invention.
Further, the present invention also provides a kind of host cell, which contains gene order, which is big
Coli cell, agrobatcerium cell or plant cell.
It is another object of the present invention to provide the purposes that said gene is used for transgene improvement crop.
Transgenic paddy rice is prepared as this field conventional technical means, and the present invention is not construed as limiting separately, and utilization is of the present invention
Gene carries out the technical solution of Transgenic Rice within protection scope of the present invention.
Realize that steps are as follows for particular technique of the invention：
One, the identification and positioning of the additional glume mutant gene EH1 of rice
The additional glume mutant eh1 of the present invention is to screen to obtain from the EMS mutagenesis bodies library of japonica rice variety Nipponbare NIP
's.In the maturity period, the anther of eh1 is observed as a result, it has been found that eh1 has additional glume, than the glume for normally having more one times.For
Separation EH1 genes, the present invention construct a target group, are hybridized with rice variety TN1 by eh1 and combine F first2Positioning
Group, then by the method for map based cloning, Primary Location is carried out to the sites EH1 using different kinds of molecules label, by its Primary Location
On the 11st chromosome, and between P1 and P8 labels.It is analyzed by the sequence between the two labels, development is new
Polymorphism mark by EH1 genes be pin-pointed to ID label P4 and P5 between about 400kb region in, by the pre- of section
Cls gene sequencing compares analysis and finds that compared with NIP, the code area of the LOC_Os11g38270 genes of eh1 has occurred single base and replaces
It changes.Blast analyses find that EH1 genes (LOC_Os11g38270) and the FON4 of forefathers' report are allele.The mutational sites eh1
Inconsistent with the mutational site of the FON4 allele of forefathers' report, the mutational site in eh1 is located at the third of code area
The 317th nucleotide A on exon replaces with G, and the 106th amino acids of coding is caused to become Arg from His.
The additional glume mutant eh1 phenotypes of rice of the present invention and other FON4 allelic variants bodies (fon4 and fon2) are
Difference.It is characterized in that：And be mainly characterized by floral organ number and the Primary branch number of fon4 mutant increase, the feature of fon2
Predominantly floral meristem increases and gynoecium number increases, however eh1 mutant is mainly shown as that floral organ number increases, such as
Foreign steamer glume and lubrication groove floral organ.
Two, the identification and functional analysis of EH1 genes
Utilize pCAMBIA1300 plasmid construction EH1 complementing vectors.Construction step：The 5 '-of EH1 genes in PCR amplification NIP
The total 4.7-kb genomic DNA fragments of UTR 2005bp to 3 '-UTR1206bp, pCAMBIA1300 carriers are connected to by the segment
Upper acquisition pCAMBIA1300-EH1 complementing vectors.
By transgenic technology, the transgenic research that has complementary functions, the results showed that restore present invention obtains eh1 is made
The transgenic paddy rice of normal glume, it was demonstrated that it is of the invention it is correct cloned EH1 genes, specify the function of EH1 genes.
In conclusion present invention separation and clone identification control flower development related gene EH1, and by complementation test into
Row gene function is verified.The present invention is improvement, enhancing rice fertility, and acceleration molecular breeding process has highly important theory
And practical significance.
Description of the drawings
The specific implementation mode of the present invention is described in further detail below in conjunction with the accompanying drawings.
Fig. 1 is the phenotype of wild type and eh1 mutant heading stage little Hua, A：The little Hua of wild type；B：In wild type little Hua
Portion floral organ；C：Eh1 mutant little Hua；D：Floral organ inside eh1 mutant little Hua.
Fig. 2 is the scanning electron microscope of wild type and eh1 mutant early stage little Hua, A：Wild type early stage little Hua；B：Eh1 mutant
Early stage little Hua.
Fig. 3 is the positioning figure of EH1 genes, A：The finely positioning of EH1 genes, utilizes eh1/TN1F2701 plants of group
Be mutated single plant, by the EH1 assignments of genes gene mapping o.11 chromosome be positioned at P4 and P5 label between；B：EH1 is defined in the areas 400kb
Domain；C：The structural schematic diagram of EH1 genes, by comparing analysis to NIP the and eh1 parental gene group DNA sequence dnas sequencing in the region,
As a result, it has been found that the 317th nucleotide A on the third exon of gene EH1 (LOC_Os11g38270) replaces with G.
Fig. 4 is pCAMBIA1300-EH1 Vector maps.
Fig. 5 is the phenotype of function complementation experiment transgenic paddy rice cp.
Specific implementation mode
With reference to specific embodiment, the present invention is described further.These descriptions are not to make to the content of present invention
Further to limit, if not specified in following embodiment, technological means used is well known to those skilled in the art
Conventional means.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
The acquisition of embodiment 1, mutant eh1 materials
By EMS mutagenesis japonica rice variety Nipponbares, a additional glume mutant eh1 is screened.The mutant
Character has stablized heredity by inbreeding of more generation, is observed under field condition and compares mutant and is shown in florescence with wild type
The phenotype of additional glume.All rice material plantations are in the biochemical institute experimental plot of Zhejiang Normal University of Jinhua, Zhejiang Province city, often
Regulate reason.
Above-mentioned EMS mutagenesises method is specially：By Nipponbare seed be immersed in concentration 0.05-0.5mol/L methyl
Germination is planted to crop field meet the additional glume screening conditions of plant by inbreeding of more generation later by sulfonic acid 30min
The additional glume mutant eh1 of conduct of (stablizing that is, meeting each strain phenotype of offspring, the condition of phenotype separation no longer occurs).
The phenotypic analysis of embodiment 2, mutant eh1
In florescence, compared with wild type Nipponbare, the Floret development of mutant eh1 goes out an additional bran shape organ, interior
Portion's floral organ stamen and gynoecium all obviously increase (Fig. 1), and the scanning electron microscope to early stage little Hua is as a result, it has been found that in early stage mutant eh1
There have been stamens and gynoecium number to increase (Fig. 2).
Embodiment 3, informative population and genetic analysis
By mutant eh1 and indica conventional rice TN1Carry out hybridization combo, F1Plant shows normal wild type phenotype, explanation
Eh1 is controlled by recessive nuclear gene.Count F2Segregating population segregation ratio (table 1), the results showed that, the plant of normal phenotype and mutant
The segregation ratio of the plant of phenotype passes through Chi-square Test close to 3:1 separation, this shows that the additional glume phenotype of eh1 is by a pair of single hidden
Property karyogene control.
The genetic analysis of 1 mutant eh1 of table
The assignment of genes gene mapping of embodiment 4, EH1 genes
The 262 pairs of SSR primer pairs mutant and TN for being uniformly distributed in 12 chromosome of rice preserved using this laboratory1
Polymorphism screening is carried out, screening 134 pairs of SSR primers has polymorphism.Then with 21 eh1/TN1Middle F2(i.e. eh1 and TN1Two
F is generated after a mixing breed1Then plant carries out selfing with the seed of F1 plant again and generates F2Plant) there is additional glume list
Strain carries out linkage analysis, the preliminary chromosome location confirmed where target gene.Genomic DNA is extracted using CTAB methods.
It is as follows：
1., weigh the rice leaf liquid nitrogen grinding powdering of 0.1g, the CTAB solution (2% (m/ of 600 μ l is then added
V) CTAB, 100mmol/L Tris-Cl, 20mmol/L EDTA, 1.4mol/L NaCl；PH8.0 the DNA extraction bufferings) prepared
Liquid, 65 DEG C of water-baths 40 minutes.Again plus the chloroform of 600 μ l:Isoamyl alcohol (24:1 volume ratio), and mixing.10,000rpm centrifugations 5
Minute, supernatant is transferred in new centrifuge tube.
2., after 1. above-mentioned steps centrifuge in the supernatant of gained plus the isopropanol of 2/3~1 times of volume precooling (to 4 DEG C),
Gently mixing to DNA precipitate.13,000rpm centrifugations 8 minutes, pour out supernatant.
3., again with the 200 μ l of alcohol of 70% (volumetric concentration) wash above-mentioned steps 2. gained DNA sediments.
4., the DNA after above-mentioned washing is dried and is dissolved in 100 μ l TE buffer solutions or pure water.
5., ultraviolet spectrophotometry detection above-mentioned steps 4. gained DNA sample concentration, 0.7% Ago-Gel
The integrality of electrophoresis detection DNA.Complete suitable DNA is used for PCR amplification, and incomplete DNA is then extracted again, until having obtained
Whole DNA.
PCR reaction systems use 10 μ L systems：1 μ L, 10 × PCR buffer solution of DNA profiling 1 μ L, forward and reverse primer (10 μ
Mol/L) 1 μ L, rTaq enzyme of each 0.5 μ L, dNTPs, 0.2 μ L, add ddH2O supplies 10 μ L.PCR amplification program is as follows：It is pre- at 94 DEG C
It is denaturalized 4min；It is denaturalized 30s at 94 DEG C, annealing 30s (temperature is different because of primer difference) at 55 DEG C~60 DEG C extends 30s at 72 DEG C,
40 cycles；Extend 10min at last 72 DEG C.4% agarose gel electrophoresis of PCR product, in gel imaging after electrophoresis
Instrument takes pictures and reads glue.The discovery of EH1 gene linkage analysis is carried out in o.11 chromosome using 134 pairs of SSR primers of above-mentioned screening
SSR marker P1 at show cascade phenomenon.New Indel labels are designed in linked marker upstream and downstream, it will with this 21 single plants
Target gene section is locked between molecular labeling P1 and P8.In this section new molecular labeling of secondary design again, with 701 F2It is single
Strain is finally by the assignment of genes gene mapping between P4 and P5 in the section of about 400kb.Primer sequence is shown in Table 2.
Molecular labeling used in table 2, the assignment of genes gene mapping
According to rice genome database (http://rice.plantbiology.msu.edu/) data information, to area
Domain interior prediction genetic analysis, find in section there are one it has been reported that floral organ number control gene FON4, the number of logging in is
LOC_Os11g38270 is expanded the cDNA of wild type and mutant using the primer in the regions covering candidate gene FON4, surveyed respectively
Sequence the result shows that, the FON4 genes of eh1 are located at the 317th nucleotide A on the third exon of code area and replace with G, lead
It causes the 106th amino acids of coding to become Arg from His, indicates that the gene is candidate gene (Fig. 3), other fon4 being reported
Allelic variant body is all large fragment deletion.
The nucleotides sequence of the additional glume mutant gene EH1 of rice is classified as SEQ ID NO：1, the additional glume mutant of rice
The amino acid sequence of corresponding wild type Nipponbare is SEQ ID NO：2.
The amino acid sequence such as SEQ ID NO of protein coded by the additional glume mutant gene EH1 of rice：Described in 3.It is wild
The amino acid sequence such as SEQ ID NO of protein coded by raw type Nipponbare：Described in 4.
Embodiment 5, Plant Transformation and functional verification
Expand the total 4.7-kb genomic DNAs of the 5 '-UTR 2005bp to 3 '-UTR 1206bp of EH1 genes in Nipponbare
Segment.Then it is connected into pEASY-Blunt Cloning Vector (TransGen Biotech companies), is being connected into later
In pCAMBIA1300 carriers (Fig. 4).
This plasmid is transferred to Agrobacterium (Agrobacterium tumefaciens) strain EHA105 by the method for electric shock
Middle rice transformation.We utilize eh1 mature embryo-derived callus, and after inducing culture culture 2 weeks, it is prosperous to select growth
The callus of Sheng is used as the receptor of conversion.It is infected with the EHA105 bacterial strains containing binary plasmid carrier (pCAMBIA1300-COLD2)
Rice callus, after being co-cultured 3 days under the conditions of dark, 25 DEG C, in the screening and culturing medium glazing containing 50mg/L Hygromycin
According to culture 14 days or so (intensity of illumination 13200LX, temperature are 32 DEG C).By the callus broken up in advance go on differential medium
(intensity of illumination 13200LX, temperature are 32 DEG C) culture obtains resistant transgenic plant in one month or so under illumination condition.To mutual
The plant cp that fills the gaps with seedlings carries out the observation and analysis of floral organ in the maturity period, it is found that the glume mutually filled the gaps with seedlings is restored to the same (figure of wild type
5)。
Remarks explanation：Every culture medium (inducing culture, screening and culturing medium, differential medium) mentioned hereinabove is
Conventional medium.
The application of embodiment 6, rice flower development gene EH1 in rice breeding
In production practice, above-mentioned EH1 genes are hybridized with conventional variety (such as Zhejiang spoke 802), the selection and breeding tool in offspring
The individual for having EH1 genotype, the individual selected in this way, floret bears mesh increase, and to improve single plant yield, (yield can be at least
It improves 5%).
Finally, it should also be noted that it is listed above be only the present invention several specific embodiments.Obviously, this hair
Bright to be not limited to above example, acceptable there are many deformations.Those skilled in the art can be from present disclosure
All deformations for directly exporting or associating, are considered as protection scope of the present invention.
Sequence table
<110>Zhejiang Normal University
<120>Rice flower development gene EH1 and its application
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<170> SIPOSequenceListing 1.0
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<211> 369
<212> DNA
<213>Rice (Oryza sativa)
<400> 1
atgggccggc tcttcttgtg tttggtggtt gcatggtgtt gggttgctct gctgcttgtt 60
gctccggtgc atggacgtgt tggcttgccc ggtgagttta gtggtgacca gaggccagtc 120
ccggcgacat cttttgatct cgtgacagag ccgaagacga agcagccgcg cggcgtgaag 180
ggcacccgca ggccgtcgtg gtcttcttgg tcgtcgacgg cgtcgagatc gtcgccgccg 240
ccggggagag gggcgccctc ggccgccgcg gcggcggagc tccggtcagt gccggccggc 300
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cggccatga 369
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<400> 2
atgggccggc tcttcttgtg tttggtggtt gcatggtgtt gggttgctct gctgcttgtt 60
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ccggcgacat cttttgatct cgtgacagag ccgaagacga agcagccgcg cggcgtgaag 180
ggcacccgca ggccgtcgtg gtcttcttgg tcgtcgacgg cgtcgagatc gtcgccgccg 240
ccggggagag gggcgccctc ggccgccgcg gcggcggagc tccggtcagt gccggccggc 300
ccggacccga tgcaccacca cggcagcccg aggcggccgg agcacgcgcg cagcaccggc 360
cggccatga 369
<210> 3
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<212> PRT
<213>Rice (Oryza sativa)
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Met Gly Arg Leu Phe Leu Cys Leu Val Val Ala Trp Cys Trp Val Ala
1 5 10 15
Leu Leu Leu Val Ala Pro Val His Gly Arg Val Gly Leu Pro Gly Glu
20 25 30
Phe Ser Gly Asp Gln Arg Pro Val Pro Ala Thr Ser Phe Asp Leu Val
35 40 45
Thr Glu Pro Lys Thr Lys Gln Pro Arg Gly Val Lys Gly Thr Arg Arg
50 55 60
Pro Ser Trp Ser Ser Trp Ser Ser Thr Ala Ser Arg Ser Ser Pro Pro
65 70 75 80
Pro Gly Arg Gly Ala Pro Ser Ala Ala Ala Ala Ala Glu Leu Arg Ser
85 90 95
Val Pro Ala Gly Pro Asp Pro Met His Arg His Gly Ser Pro Arg Arg
100 105 110
Pro Glu His Ala Arg Ser Thr Gly Arg Pro
115 120
<210> 4
<211> 122
<212> PRT
<213>Rice (Oryza sativa)
<400> 4
Met Gly Arg Leu Phe Leu Cys Leu Val Val Ala Trp Cys Trp Val Ala
1 5 10 15
Leu Leu Leu Val Ala Pro Val His Gly Arg Val Gly Leu Pro Gly Glu
20 25 30
Phe Ser Gly Asp Gln Arg Pro Val Pro Ala Thr Ser Phe Asp Leu Val
35 40 45
Thr Glu Pro Lys Thr Lys Gln Pro Arg Gly Val Lys Gly Thr Arg Arg
50 55 60
Pro Ser Trp Ser Ser Trp Ser Ser Thr Ala Ser Arg Ser Ser Pro Pro
65 70 75 80
Pro Gly Arg Gly Ala Pro Ser Ala Ala Ala Ala Ala Glu Leu Arg Ser
85 90 95
Val Pro Ala Gly Pro Asp Pro Met His His His Gly Ser Pro Arg Arg
100 105 110
Pro Glu His Ala Arg Ser Thr Gly Arg Pro
115 120
Claims (3)
1. rice flower development gene EH1, it is characterised in that：The nucleotide sequence such as SEQ ID of the flower development gene EH1 of rice
NO：Shown in 1.
2. applications of the rice flower development gene EH1 as described in claim 1 in rice breeding.
3. application according to claim 2, it is characterised in that：For improving rice yield.
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| CN201810344126.9A CN108570474B (en) | 2018-04-17 | 2018-04-17 | Rice flower development gene EH1 and application thereof |
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| CN108570474B CN108570474B (en) | 2021-03-02 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109295071A (en) * | 2018-10-12 | 2019-02-01 | 福建省农业科学院生物技术研究所 | Protein and the application of a kind of rice flower organ developmental regulation gene PEH1 and its coding |
| CN109456984A (en) * | 2018-12-30 | 2019-03-12 | 浙江师范大学 | Rice glume development gene AH1 and its application |
| CN110724694A (en) * | 2019-11-28 | 2020-01-24 | 华南农业大学 | Rice fertility gene SAW1 and application thereof |
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| CN101363025A (en) * | 2008-08-12 | 2009-02-11 | 华中农业大学 | Histone demethylase gene OsJMJ706 of rice, encoding protein thereof and applications |
| CN101798342A (en) * | 2010-03-11 | 2010-08-11 | 中国农业大学 | Rice glume development related protein TRI1 and encoding gene and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101363025A (en) * | 2008-08-12 | 2009-02-11 | 华中农业大学 | Histone demethylase gene OsJMJ706 of rice, encoding protein thereof and applications |
| CN101798342A (en) * | 2010-03-11 | 2010-08-11 | 中国农业大学 | Rice glume development related protein TRI1 and encoding gene and application thereof |
Non-Patent Citations (2)
| Title |
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| BUELL,C.R.等: "Oryza sativa(japonica cultivar-group)chromosome 11,complete sequence", 《NCBI》 * |
| CHU,H.等: "Oryza sativa(japonica cultivar-group)floral organ number 4(FON4)mRNA，complete cds", 《NCBI》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109295071A (en) * | 2018-10-12 | 2019-02-01 | 福建省农业科学院生物技术研究所 | Protein and the application of a kind of rice flower organ developmental regulation gene PEH1 and its coding |
| CN109295071B (en) * | 2018-10-12 | 2021-07-13 | 福建省农业科学院生物技术研究所 | Rice flower organ development regulation gene PEH1, and encoded protein and application thereof |
| CN109456984A (en) * | 2018-12-30 | 2019-03-12 | 浙江师范大学 | Rice glume development gene AH1 and its application |
| CN109456984B (en) * | 2018-12-30 | 2021-05-25 | 浙江师范大学 | Rice glume development gene AH1 and application thereof |
| CN110724694A (en) * | 2019-11-28 | 2020-01-24 | 华南农业大学 | Rice fertility gene SAW1 and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108570474B (en) | 2021-03-02 |
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