CN108558880A - A kind of production method for extracting matrine from kuh-seng - Google Patents
A kind of production method for extracting matrine from kuh-seng Download PDFInfo
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- CN108558880A CN108558880A CN201810454342.9A CN201810454342A CN108558880A CN 108558880 A CN108558880 A CN 108558880A CN 201810454342 A CN201810454342 A CN 201810454342A CN 108558880 A CN108558880 A CN 108558880A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
- C07D471/22—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed systems contains four or more hetero rings
Abstract
The invention discloses a kind of from kuh-seng extracts the production method of matrine, includes the following steps：The first step takes 100kg kuh-sengs, is crushed to 10 mesh, obtains Lightyellow Sophora Root；Second step is added sodium pyrosulfite in the concentrate obtained into step 1, obtains reaction solution, reduction reaction is carried out at a temperature of reaction solution is placed on 50 DEG C, and pass through TLC method tracing detections；Third walks, and by the reaction solution of the end reduction reaction of gained in step 2, is pumped into economic benefits and social benefits decompression concentrator with vacuum, obtains 12.5kg biology total alkali crude products；4th step, the biology total alkali crude product that will be obtained in step 3, is dissolved with ethyl alcohol, obtains lysate, and aluminium oxide is added into lysate and mixes thoroughly and dries, obtains solidfied material, obtains matrine crude product；Obtained ethyl acetate solution is concentrated into 720L by the 5th step with economic benefits and social benefits decompression concentrator, and the ethyl acetate solution concentrated placement is crystallized at room temperature, obtains 98% matrine.
Description
Technical field
The present invention relates to matrine extracting method technical field, more particularly to a kind of production for extracting matrine from kuh-seng
Method.
Background technology
Kuh-seng is the dry root of legume kuh-seng, and main component includes that oxymatrine, matrine, sophocarpine, Chinese scholartree are fixed
A variety of composition of alkaloids such as alkali, aloperine, sophoramine, sparteine, Iosmatrine, modern pharmacology experiment and clinic have been demonstrate,proved
There are many physiological activity for bright matrine, especially have inhibiting effect to hepatitis, vital myocarditis, tumour.
Domestic existing matrine extraction process, main extraction and purification and matrine including kuh-seng biology total alkali are from kuh-seng
Two big step of isolation and purification in total alkali, the problems of the technique are:First, aqueous extraction is less efficient；Second is that also
Former process is weaker, can only be by the oxidized state of alkaloid at reduction-state, cannot will alkaloid similar with matrine structure
(such as:Sophoridine) it is reduced into matrine, in this way, the yield of matrine on the one hand cannot be improved, on the other hand make follow-up matrine
More difficult from the separation process in Banlangen, technical process is cumbersome；Third, the purifying organic solvent of kuh-seng biology total alkali
Extraction and back extraction, technical process toxicity is big, and production operation is not safe enough.
Invention content
The purpose of the present invention is disclosing a kind of production method for extracting matrine from kuh-seng, for existing kuh-seng alkali carries
Aqueous existing for taking technique is extracted less efficient, and reduction process is weaker, can only by the oxidized state of alkaloid at reduction-state,
Cannot will alkaloid similar with matrine structure (such as:Sophoridine) it is reduced into matrine, technical process is cumbersome, and kuh-seng biology is total
The purifying of alkali is extracted and is stripped with organic solvent, and technical process toxicity is big, not safe enough the problem of production operation.
To achieve the goals above, technical scheme of the present invention is specific as follows：
A kind of production method for extracting matrine from kuh-seng, includes the following steps：
The first step takes 100kg kuh-sengs, is crushed to 10 mesh, obtains Lightyellow Sophora Root, is then packed into back obtained Lightyellow Sophora Root
It flows in extractor, 70% ethyl alcohol 500L is added to refluxing extraction device, ethyl alcohol is made to be impregnated with Lightyellow Sophora Root, at a temperature of 60 DEG C,
It is extracted 3 times with 75% alcohol reflux, 2 hours every time, material residue discarded, and obtained 1500L extracting solutions, by extracting solution through 200 mesh precision
After filter, filtered extracting solution is pumped into economic benefits and social benefits decompression concentrator with vacuum pump, 60 DEG C or less are concentrated into no alcohol,
Obtain 100kg concentrates；
Second step is added in the concentrate that is obtained into step 1 of ratio according to extraction kuh-seng biology total alkali 6% burnt sub-
Sodium sulphate obtains reaction solution, reduction reaction is carried out at a temperature of reaction solution is placed on 50 DEG C, and pass through TLC method tracing detections, step
It is rapid as follows：
(1) using 2mg/ml matrines and 2mh/ml oxidative biologicals alkali as reference substance, the reaction solution with above-mentioned reduction reaction is
Test sample；
(2) color developing agent is prepared, takes basic bismuth nitrate 0.85g to be dissolved in 10ml glacial acetic acid and is tried with the mixed liquor of 40ml water
Potassium iodide 10g is dissolved in 20ml water by agent a, obtains reagent b, and reagent a and reagent b mixed in equal amounts are set in brown bottle, stored up
Standby liquid, takes 1ml storing solutions, 2ml glacial acetic acid to be mixed with 10ml water, obtains color developing agent；
(3) solvent is prepared, by benzene, acetone, ethyl acetate and concentrated ammonia liquor according to volume ratio 2:3:4:0.2 mixing, obtains
Solvent；
(4) capillary is used to put matrine described in upper step (1), oxymatrine on G type silica gel plates, for examination respectively
Each semicanal of product is unfolded with the solvent that is obtained in step (3), dries up G type silica gel plates, with thin layer watering can to spraying step on silica gel plate
Suddenly the color developing agent obtained in (2) shows salmon patch at this point, at matrine and oxymatrine drop on G type silica gel plates
Point, and at the end of reduction reaction, the spot of oxymatrine will vanish from sight in test sample, therefore, until it is observed that
When orange red spot identical with matrine is presented in reaction solution, then illustrate that the reduction reaction of reaction solution terminates, obtains terminating reduction
The reaction solution of reaction；
Third walks, and by the reaction solution of the end reduction reaction of gained in step 2, is pumped into economic benefits and social benefits with vacuum and is concentrated under reduced pressure
It in device, is concentrated under reduced pressure at 80 DEG C, obtains medicinal extract 50kg, with 1% sodium hydroxide by the pH value of medicinal extract, be adjusted to PH=11, later,
Into medicinal extract plus dimethylbenzene 150L is extracted three times, obtains 450L extract liquors, by the hydrochloric acid water back extraction three of 3 times of amounts 2% of extract liquor
It is secondary, water layer is collected, 1350L aqueous hydrochloric acid solutions are obtained, 2% sodium hydroxide solution is added into aqueous hydrochloric acid solution, by aqueous hydrochloric acid solution
PH value, be adjusted to PH=9, finally, placement crystallize at room temperature for 24 hours, filtering for crystallizing obtains 12.5kg biology total alkali crude products；
4th step, the biology total alkali crude product that will be obtained in step 3, is dissolved with the ethyl alcohol of 24L75%, is dissolved
72kg is added into lysate for liquid, and the aluminium oxide of 100-200 mesh, which is mixed thoroughly, to be dried, and solidfied material is obtained, successively to 80 × 420 column layers
It analyses and is packed into 1440kg aluminium oxide and solidfied material in column, eluted by eluant, eluent of ethyl acetate, the TLC methods in step 2 are used in combination
Tracing detection, wherein using biology total alkali as reference substance, using eluant, eluent as test sample, using the color developing agent that is prepared in step 2 and
Solvent starts to collect eluant, eluent when occurring the orange red spot of biology total alkali in eluent, when biology total alkali in eluant, eluent
Orange red spot disappear when stop collect, obtain 4500L eluents, eluent be concentrated under reduced pressure at 0.09mp, 40 DEG C, is obtained
To matrine crude product；
5th step, the matrine crude product that will be obtained in step 4 are dissolved three times with 720L ethyl acetate at 70 DEG C respectively,
2160L ethyl acetate solutions are obtained, obtained ethyl acetate solution is concentrated into 720L with economic benefits and social benefits decompression concentrator, will be concentrated
Ethyl acetate solution placement crystallize at room temperature for 24 hours, for 24 hours after filtering for crystallizing, obtain 98% matrine 2.3kg.
The beneficial effects of the invention are as follows：
(1) present invention employs alcohol reflux extraction, extraction time is shortened, yield is improved；
(2) present invention carries out strong reduction with sodium pyrosulfite as reducing agent, and it is similar that oxymatrine is reduced into structure
Sophoridine, not only increase the yield of matrine, also reduce the difficulty of later separation matrine, avoid organic solvent
It uses, simplifies separation process, with high income, purity is good, with short production cycle, technical process is simple, operation is safer
Advantage；
(3) present invention can not only be by the oxidized state of alkaloid at reduction-state, moreover it is possible to will be similar with matrine structure
Alkaloid is (such as:Sophoridine) it is reduced into matrine；
Specific implementation mode
Embodiment 1
A kind of production method for extracting matrine from kuh-seng, includes the following steps：
The first step takes 100kg kuh-sengs, is crushed to 10 mesh, obtains Lightyellow Sophora Root, is then packed into back obtained Lightyellow Sophora Root
It flows in extractor, 70% ethyl alcohol 500L is added to refluxing extraction device, ethyl alcohol is made to be impregnated with Lightyellow Sophora Root, at a temperature of 60 DEG C,
It is extracted 3 times with 75% alcohol reflux, 2 hours every time, material residue discarded, and obtained 1500L extracting solutions, by extracting solution through 200 mesh precision
After filter, filtered extracting solution is pumped into economic benefits and social benefits decompression concentrator with vacuum pump, 60 DEG C or less are concentrated into no alcohol,
Obtain 100kg concentrates；
Second step is added in the concentrate that is obtained into step 1 of ratio according to extraction kuh-seng biology total alkali 6% burnt sub-
Sodium sulphate obtains reaction solution, reduction reaction is carried out at a temperature of reaction solution is placed on 50 DEG C, and pass through TLC method tracing detections, step
It is rapid as follows：
(1) using 2mg/ml matrines and 2mh/ml oxidative biologicals alkali as reference substance, the reaction solution with above-mentioned reduction reaction is
Test sample；
(2) color developing agent is prepared, takes basic bismuth nitrate 0.85g to be dissolved in 10ml glacial acetic acid and is tried with the mixed liquor of 40ml water
Potassium iodide 10g is dissolved in 20ml water by agent a, obtains reagent b, and reagent a and reagent b mixed in equal amounts are set in brown bottle, stored up
Standby liquid, takes 1ml storing solutions, 2ml glacial acetic acid to be mixed with 10ml water, obtains color developing agent；
(3) solvent is prepared, by benzene, acetone, ethyl acetate and concentrated ammonia liquor according to volume ratio 2:3:4:0.2 mixing, obtains
Solvent；
(4) capillary is used to put matrine described in upper step (1), oxymatrine on G type silica gel plates, for examination respectively
Each semicanal of product is unfolded with the solvent that is obtained in step (3), dries up G type silica gel plates, with thin layer watering can to spraying step on silica gel plate
Suddenly the color developing agent obtained in (2) shows salmon patch at this point, at matrine and oxymatrine drop on G type silica gel plates
Point, and at the end of reduction reaction, the spot of oxymatrine will vanish from sight in test sample, therefore, until it is observed that
When orange red spot identical with matrine is presented in reaction solution, then illustrate that the reduction reaction of reaction solution terminates, obtains terminating reduction
The reaction solution of reaction；
Third walks, and by the reaction solution of the end reduction reaction of gained in step 2, is pumped into economic benefits and social benefits with vacuum and is concentrated under reduced pressure
It in device, is concentrated under reduced pressure at 80 DEG C, obtains medicinal extract 50kg, with 1% sodium hydroxide by the pH value of medicinal extract, be adjusted to PH=11, later,
Into medicinal extract plus dimethylbenzene 150L is extracted three times, obtains 450L extract liquors, by the hydrochloric acid water back extraction three of 3 times of amounts 2% of extract liquor
It is secondary, water layer is collected, 1350L aqueous hydrochloric acid solutions are obtained, 2% sodium hydroxide solution is added into aqueous hydrochloric acid solution, by aqueous hydrochloric acid solution
PH value, be adjusted to PH=9, finally, placement crystallize at room temperature for 24 hours, filtering for crystallizing obtains 12.5kg biology total alkali crude products；
4th step, the biology total alkali crude product that will be obtained in step 3, is dissolved with the ethyl alcohol of 24L75%, is dissolved
72kg is added into lysate for liquid, and the aluminium oxide of 100 mesh, which is mixed thoroughly, to be dried, and solidfied material is obtained, successively to 80 × 420 column chromatography columns
Middle loading 1440kg aluminium oxide and solidfied material, are eluted by eluant, eluent of ethyl acetate, and the TLC methods in step 2 is used in combination to track
Detection, wherein using biology total alkali as reference substance, using eluant, eluent as test sample, use the color developing agent and expansion prepared in step 2
Agent starts to collect eluant, eluent when occurring the orange red spot of biology total alkali in eluent, when the orange of biology total alkali in eluant, eluent
Stop collecting when punctation disappears, obtains 4500L eluents, eluent is concentrated under reduced pressure at 0.09mp, 40 DEG C, obtains hardship
Join alkali crude product；
5th step, the matrine crude product that will be obtained in step 4 are dissolved three times with 720L ethyl acetate at 70 DEG C respectively,
2160L ethyl acetate solutions are obtained, obtained ethyl acetate solution is concentrated into 720L with economic benefits and social benefits decompression concentrator, will be concentrated
Ethyl acetate solution placement crystallize at room temperature for 24 hours, for 24 hours after filtering for crystallizing, obtain 98% matrine 2.3kg.
Embodiment 2
A kind of production method for extracting matrine from kuh-seng, includes the following steps：
The first step takes 100kg kuh-sengs, is crushed to 10 mesh, obtains Lightyellow Sophora Root, is then packed into back obtained Lightyellow Sophora Root
It flows in extractor, 70% ethyl alcohol 500L is added to refluxing extraction device, ethyl alcohol is made to be impregnated with Lightyellow Sophora Root, at a temperature of 60 DEG C,
It is extracted 3 times with 75% alcohol reflux, 2 hours every time, material residue discarded, and obtained 1500L extracting solutions, by extracting solution through 200 mesh precision
After filter, filtered extracting solution is pumped into economic benefits and social benefits decompression concentrator with vacuum pump, 60 DEG C or less are concentrated into no alcohol,
Obtain 100kg concentrates；
Second step is added in the concentrate that is obtained into step 1 of ratio according to extraction kuh-seng biology total alkali 6% burnt sub-
Sodium sulphate obtains reaction solution, reduction reaction is carried out at a temperature of reaction solution is placed on 50 DEG C, and pass through TLC method tracing detections, step
It is rapid as follows：
(1) using 2mg/ml matrines and 2mh/ml oxidative biologicals alkali as reference substance, the reaction solution with above-mentioned reduction reaction is
Test sample；
(2) color developing agent is prepared, takes basic bismuth nitrate 0.85g to be dissolved in 10ml glacial acetic acid and is tried with the mixed liquor of 40ml water
Potassium iodide 10g is dissolved in 20ml water by agent a, obtains reagent b, and reagent a and reagent b mixed in equal amounts are set in brown bottle, stored up
Standby liquid, takes 1ml storing solutions, 2ml glacial acetic acid to be mixed with 10ml water, obtains color developing agent；
(3) solvent is prepared, by benzene, acetone, ethyl acetate and concentrated ammonia liquor according to volume ratio 2:3:4:0.2 mixing, obtains
Solvent；
(4) capillary is used to put matrine described in upper step (1), oxymatrine on G type silica gel plates, for examination respectively
Each semicanal of product is unfolded with the solvent that is obtained in step (3), dries up G type silica gel plates, with thin layer watering can to spraying step on silica gel plate
Suddenly the color developing agent obtained in (2) shows salmon patch at this point, at matrine and oxymatrine drop on G type silica gel plates
Point, and at the end of reduction reaction, the spot of oxymatrine will vanish from sight in test sample, therefore, until it is observed that
When orange red spot identical with matrine is presented in reaction solution, then illustrate that the reduction reaction of reaction solution terminates, obtains terminating reduction
The reaction solution of reaction；
Third walks, and by the reaction solution of the end reduction reaction of gained in step 2, is pumped into economic benefits and social benefits with vacuum and is concentrated under reduced pressure
It in device, is concentrated under reduced pressure at 80 DEG C, obtains medicinal extract 50kg, with 1% sodium hydroxide by the pH value of medicinal extract, be adjusted to PH=11, later,
Into medicinal extract plus dimethylbenzene 150L is extracted three times, obtains 450L extract liquors, by the hydrochloric acid water back extraction three of 3 times of amounts 2% of extract liquor
It is secondary, water layer is collected, 1350L aqueous hydrochloric acid solutions are obtained, 2% sodium hydroxide solution is added into aqueous hydrochloric acid solution, by aqueous hydrochloric acid solution
PH value, be adjusted to PH=9, finally, placement crystallize at room temperature for 24 hours, filtering for crystallizing obtains 12.5kg biology total alkali crude products；
4th step, the biology total alkali crude product that will be obtained in step 3, is dissolved with the ethyl alcohol of 24L75%, is dissolved
72kg is added into lysate for liquid, and the aluminium oxide of 200 mesh, which is mixed thoroughly, to be dried, and solidfied material is obtained, successively to 80 × 420 column chromatography columns
Middle loading 1440kg aluminium oxide and solidfied material, are eluted by eluant, eluent of ethyl acetate, and the TLC methods in step 2 is used in combination to track
Detection, wherein using biology total alkali as reference substance, using eluant, eluent as test sample, use the color developing agent and expansion prepared in step 2
Agent starts to collect eluant, eluent when occurring the orange red spot of biology total alkali in eluent, when the orange of biology total alkali in eluant, eluent
Stop collecting when punctation disappears, obtains 4500L eluents, eluent is concentrated under reduced pressure at 0.09mp, 40 DEG C, obtains hardship
Join alkali crude product；
5th step, the matrine crude product that will be obtained in step 4 are dissolved three times with 720L ethyl acetate at 70 DEG C respectively,
2160L ethyl acetate solutions are obtained, obtained ethyl acetate solution is concentrated into 720L with economic benefits and social benefits decompression concentrator, will be concentrated
Ethyl acetate solution placement crystallize at room temperature for 24 hours, for 24 hours after filtering for crystallizing, obtain 98% matrine 2.3kg.
Claims (1)
1. a kind of production method for extracting matrine from kuh-seng, which is characterized in that include the following steps：
The first step takes 100kg kuh-sengs, is crushed to 10 mesh, obtains Lightyellow Sophora Root, and obtained Lightyellow Sophora Root, which is then packed into reflux, carries
It takes in device, 70% ethyl alcohol 500L is added to refluxing extraction device, ethyl alcohol is made to be impregnated with Lightyellow Sophora Root, at a temperature of 60 DEG C, use
75% alcohol reflux extracts 3 times, and 2 hours every time, material residue discarded, and obtained 1500L extracting solutions, by extracting solution through 200 mesh precision mistakes
After filter, filtered extracting solution is pumped into economic benefits and social benefits decompression concentrator with vacuum pump, 60 DEG C or less are concentrated into no alcohol, are obtained
To 100kg concentrates；
Pyrosulfurous acid is added in the concentrate that is obtained into step 1 of ratio according to extraction kuh-seng biology total alkali 6% in second step
Sodium obtains reaction solution, reduction reaction is carried out at a temperature of reaction solution is placed on 50 DEG C, and by TLC method tracing detections, step is such as
Under：
(1) it is for examination with the reaction solution of above-mentioned reduction reaction using 2mg/ml matrines and 2mh/ml oxidative biologicals alkali as reference substance
Product；
(2) color developing agent is prepared, takes basic bismuth nitrate 0.85g to be dissolved in 10ml glacial acetic acid and obtains reagent a with the mixed liquor of 40ml water,
Potassium iodide 10g is dissolved in 20ml water, reagent b is obtained, reagent a and reagent b mixed in equal amounts are set in brown bottle, laid in
Liquid takes 1ml storing solutions, 2ml glacial acetic acid to be mixed with 10ml water, obtains color developing agent；
(3) solvent is prepared, by benzene, acetone, ethyl acetate and concentrated ammonia liquor according to volume ratio 2:3:4:0.2 mixing, is unfolded
Agent；
(4) use matrine, oxymatrine, test sample that capillary is put on G type silica gel plates described in upper step (1) each respectively
Semicanal is unfolded with the solvent that is obtained in step (3), dries up G type silica gel plates, with thin layer watering can to spraying step on silica gel plate
(2) color developing agent obtained in shows orange red spot at this point, at matrine and oxymatrine drop on G type silica gel plates,
And at the end of reduction reaction, the spot of oxymatrine will vanish from sight in test sample, therefore, until it is observed that reaction
When orange red spot identical with matrine is presented in liquid, then illustrate that the reduction reaction of reaction solution terminates, obtains terminating reduction reaction
Reaction solution；
Third walks, and the reaction solution of the end reduction reaction of gained in step 2 is pumped into vacuum in economic benefits and social benefits decompression concentrator,
It is concentrated under reduced pressure at 80 DEG C, obtains medicinal extract 50kg, with 1% sodium hydroxide by the pH value of medicinal extract, PH=11 is adjusted to, later, to leaching
In cream plus dimethylbenzene 150L is extracted three times, obtains 450L extract liquors, three times by the hydrochloric acid water back extraction of 3 times of amounts 2% of extract liquor, is received
Collect water layer, obtain 1350L aqueous hydrochloric acid solutions, 2% sodium hydroxide solution is added into aqueous hydrochloric acid solution, by the PH of aqueous hydrochloric acid solution
Value, is adjusted to PH=9, and finally, placement crystallizes for 24 hours at room temperature, and filtering for crystallizing obtains 12.5kg biology total alkali crude products；
4th step, the biology total alkali crude product that will be obtained in step 3, is dissolved with the ethyl alcohol of 24L75%, obtains lysate, to
72kg is added in lysate, the aluminium oxide of 100-200 mesh, which is mixed thoroughly, to be dried, and solidfied material is obtained, successively into 80 × 420 column chromatography columns
It is packed into 1440kg aluminium oxide and solidfied material, is eluted by eluant, eluent of ethyl acetate, the TLC methods in step 2 is used in combination to track inspection
It surveys, wherein using biology total alkali as reference substance, using eluant, eluent as test sample, using the color developing agent and solvent prepared in step 2,
Start when occurring the orange red spot of biology total alkali in eluent collect eluant, eluent, when in eluant, eluent biology total alkali it is orange red
Stop collecting when spot disappears, obtains 4500L eluents, eluent is concentrated under reduced pressure at 0.09mp, 40 DEG C, obtains matrine
Crude product；
5th step, the matrine crude product that will be obtained in step 4 are dissolved three times at 70 DEG C with 720L ethyl acetate, are obtained respectively
Obtained ethyl acetate solution is concentrated into 720L, the second that will have been concentrated by 2160L ethyl acetate solutions with economic benefits and social benefits decompression concentrator
Acetate solution placement crystallizes for 24 hours at room temperature, and rear filtering for crystallizing, obtains 98% matrine 2.3kg for 24 hours.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109575009A (en) * | 2018-12-26 | 2019-04-05 | 成都普思生物科技股份有限公司 | The new matrine of 17- hydroxyl and its extracting method and application |
| CN110338196A (en) * | 2019-06-29 | 2019-10-18 | 中国农业科学院兰州兽医研究所 | A kind of compound botanical preparation for killing tick |
| CN114591329A (en) * | 2022-03-03 | 2022-06-07 | 北京岳达生物科技有限公司 | Mutual transformation method of sophora flavescens effective components |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1453276A (en) * | 2003-05-22 | 2003-11-05 | 王答祺 | Prepn of matrine, oxymatrine and sophoxidine from flavescent sophora root |
| CN105237537A (en) * | 2015-11-16 | 2016-01-13 | 陈卫斌 | Method for preparing matrine and sophoridine in sophora alopecuroides |
| CN106188056A (en) * | 2016-07-19 | 2016-12-07 | 广州中大南沙科技创新产业园有限公司 | A kind of extracting method of matrine |
| CN107827889A (en) * | 2017-11-06 | 2018-03-23 | 广西大学 | The purification process of matrine in a kind of subprostrate sophora |
-
2018
- 2018-05-14 CN CN201810454342.9A patent/CN108558880A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1453276A (en) * | 2003-05-22 | 2003-11-05 | 王答祺 | Prepn of matrine, oxymatrine and sophoxidine from flavescent sophora root |
| CN105237537A (en) * | 2015-11-16 | 2016-01-13 | 陈卫斌 | Method for preparing matrine and sophoridine in sophora alopecuroides |
| CN106188056A (en) * | 2016-07-19 | 2016-12-07 | 广州中大南沙科技创新产业园有限公司 | A kind of extracting method of matrine |
| CN107827889A (en) * | 2017-11-06 | 2018-03-23 | 广西大学 | The purification process of matrine in a kind of subprostrate sophora |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109575009A (en) * | 2018-12-26 | 2019-04-05 | 成都普思生物科技股份有限公司 | The new matrine of 17- hydroxyl and its extracting method and application |
| CN109575009B (en) * | 2018-12-26 | 2020-04-14 | 成都普思生物科技股份有限公司 | 17-hydroxy new matrine and its extraction method and application |
| CN110338196A (en) * | 2019-06-29 | 2019-10-18 | 中国农业科学院兰州兽医研究所 | A kind of compound botanical preparation for killing tick |
| CN114591329A (en) * | 2022-03-03 | 2022-06-07 | 北京岳达生物科技有限公司 | Mutual transformation method of sophora flavescens effective components |
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