CN108552534A - With the complex polysaccharide electuary and preparation method thereof for adjusting intestinal flora function - Google Patents
With the complex polysaccharide electuary and preparation method thereof for adjusting intestinal flora function Download PDFInfo
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- CN108552534A CN108552534A CN201810307278.1A CN201810307278A CN108552534A CN 108552534 A CN108552534 A CN 108552534A CN 201810307278 A CN201810307278 A CN 201810307278A CN 108552534 A CN108552534 A CN 108552534A
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- intestinal flora
- complex polysaccharide
- inulin
- adjusting intestinal
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 131
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 130
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 130
- 230000000968 intestinal effect Effects 0.000 title claims abstract description 128
- 238000002360 preparation method Methods 0.000 title claims abstract description 68
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 claims abstract description 85
- 229920001202 Inulin Polymers 0.000 claims abstract description 83
- 229940029339 inulin Drugs 0.000 claims abstract description 83
- 238000012545 processing Methods 0.000 claims abstract description 72
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- 239000000706 filtrate Substances 0.000 claims abstract description 41
- 239000012530 fluid Substances 0.000 claims abstract description 34
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 26
- 244000298479 Cichorium intybus Species 0.000 claims abstract description 24
- 235000007542 Cichorium intybus Nutrition 0.000 claims abstract description 24
- 238000000926 separation method Methods 0.000 claims abstract description 24
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- 238000005292 vacuum distillation Methods 0.000 claims abstract description 19
- 238000004806 packaging method and process Methods 0.000 claims abstract description 10
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 claims description 77
- 238000006243 chemical reaction Methods 0.000 claims description 50
- 239000012141 concentrate Substances 0.000 claims description 48
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 48
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 41
- 102000004190 Enzymes Human genes 0.000 claims description 31
- 108090000790 Enzymes Proteins 0.000 claims description 31
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 30
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 27
- 239000007787 solid Substances 0.000 claims description 27
- 238000001976 enzyme digestion Methods 0.000 claims description 24
- 238000003756 stirring Methods 0.000 claims description 23
- 230000000694 effects Effects 0.000 claims description 20
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 18
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- 238000000034 method Methods 0.000 claims description 17
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 16
- 239000013049 sediment Substances 0.000 claims description 16
- 229930003427 Vitamin E Natural products 0.000 claims description 15
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 15
- 235000019165 vitamin E Nutrition 0.000 claims description 15
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- 239000011709 vitamin E Substances 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 13
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 claims description 12
- 241000228245 Aspergillus niger Species 0.000 claims description 11
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- 230000008014 freezing Effects 0.000 claims description 10
- 108090000145 Bacillolysin Proteins 0.000 claims description 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 9
- 102000035092 Neutral proteases Human genes 0.000 claims description 9
- 108091005507 Neutral proteases Proteins 0.000 claims description 9
- 238000004140 cleaning Methods 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 9
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- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 8
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 8
- 238000005119 centrifugation Methods 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 6
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 6
- 230000035484 reaction time Effects 0.000 claims description 5
- 241000588921 Enterobacteriaceae Species 0.000 claims description 4
- 239000002893 slag Substances 0.000 claims 2
- 210000000577 adipose tissue Anatomy 0.000 abstract description 13
- 208000008589 Obesity Diseases 0.000 abstract description 11
- 235000020824 obesity Nutrition 0.000 abstract description 11
- 208000028774 intestinal disease Diseases 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 6
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- 238000004108 freeze drying Methods 0.000 abstract description 4
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- 229940079593 drug Drugs 0.000 abstract description 2
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- 229920001542 oligosaccharide Polymers 0.000 description 4
- 150000002482 oligosaccharides Chemical class 0.000 description 4
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- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 2
- 229920001353 Dextrin Polymers 0.000 description 2
- 239000004375 Dextrin Substances 0.000 description 2
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- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
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- 210000000936 intestine Anatomy 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
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- 241000186660 Lactobacillus Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
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- 230000004151 fermentation Effects 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000007366 host health Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 235000021590 normal diet Nutrition 0.000 description 1
- 230000008855 peristalsis Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920000570 polyether Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
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- 229920005989 resin Polymers 0.000 description 1
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- 210000000813 small intestine Anatomy 0.000 description 1
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- 125000001174 sulfone group Chemical group 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
With the complex polysaccharide electuary and preparation method thereof for adjusting intestinal flora function, it belongs to food processing technology field.The present invention weighs 50~1000 parts of chitin, 50~1000 parts of fresh witloof, by decentralized processing in high shear dispersion machine, it is then transferred into enzymatic vessel enzymolysis, it is filtered later using Ultra filtration membrane equipment, filtered filtrate enters back into nanofiltration membrane separation plant and is filtered, by the vacuum distillation of filtered trapped fluid, freeze-drying, vacuum seal bag packaging is made with the complex polysaccharide electuary for adjusting intestinal flora function.The present invention has the function of more superior adjusting intestinal microflora compared to single chitosan or inulin, there is remarkable result to adjusting body fat, it can prevent and treat fat and adjust the intestinal disorder caused by obesity, food, drug daily can be made an addition to, has the function of health care on the basis of improving mouthfeel.
Description
Technical field
The invention belongs to food processing technology fields；More particularly to the complex polysaccharide electuary for adjusting intestinal flora function
And preparation method thereof.
Background technology
The mixture that the natural inulin extracted from jerusalem artichoke is made of the oligosaccharide of different polymerization degree is white without fixed
Shape, powder easy to moisture absorption.Inulin easily disperses in water, and hygroscopicity is strong, is easy to lump, micro-strip sweet taste.Synanthrin can be in the solution
The freezing point for reducing water, improves the boiling point of water.Inulin is difficult to by human consumption.Synanthrin will not disappear in oral cavity, stomach, small intestine
Change and decompose, can only be decomposed by Partial fermentations such as Bifidobacteriums in colon and generate a small amount of heat, since its calorific value is low, it is possible to
Sweetener as diabetic.In addition, inulin can inhibit the synthesis of aliphatic acid and cholesterol in liver, it may have reduce blood
Fat, blood glucose and the effect for improving energetic supersession, secondly, synanthrin inhibit harmful bacteria growth, to be beneficial to the prebiotic of host health
Element effect.Synanthrin can also promote the absorption of metal ion such as calcium, magnesium etc., and additionally aid improvement intestinal environment, reduce intestines
The generation of corrupt substance in road, energy strengthening gastrointestinal peristalsis, improves functions of intestines and stomach, increases digestion and appetite, improves immunity of organism work(
Energy.
Chitosan oligosaccharide is called Chitosan poly oligosaccharide, chitin oligo saccharide, chitosan oligomer, and scientific name β-Isosorbide-5-Nitrae-oligosaccharides-gucosamine, is by shell
A kind of degree of polymerization that glycan is degraded through special biological enzyme technology oligosaccharide product between 2~20 is water-soluble preferable, work(
Low molecular weight product big, that bioactivity is high can be acted on.The amine-based basic group of chitosan oligosaccharide can neutralize the acid that cancer cell releases
Property substance, changing cancer cell survives relied on microenvironment, make pH value of human body to alkaline direction movement 0.5 (restore to
7.35-7.45 is horizontal), the activity of macrophage is improved, increases the quantity of macrophage, therefore can effectively eliminate
Cancer cell.Meanwhile correlative study also indicates that：Chitosan oligosaccharide is unique positively charged dietary fiber, chitosan oligosaccharide conduct in nature
Prebiotics promote growth of probiotics in enteron aisle, and the beneficial bacteriums such as internal lactic acid bacteria, Bifidobacterium can be made largely to be proliferated, and inhibit large intestine
The growth of the harmful bacterias such as bacillus, Salmonella, staphylococcus aureus makes internal Tiny ecosystem reach natural equilibrium, to improve stomach
The normal physiological function of enteron aisle.
Product on the market is simple synanthrin, simple chitosan oligosaccharide now, but can integrate the preparation process of the two
Together, and being not yet reported that for multi-functional complex polysaccharide electuary is made.
Invention content
It is an object of the present invention to provide a kind of complex polysaccharide electuaries and preparation method thereof for having and adjusting intestinal flora function.
The invention is realized by the following technical scheme：
A kind of preparation method with the complex polysaccharide electuary for adjusting intestinal flora function, includes the following steps：
Step 1, the chitin for weighing 50~1000 parts respectively according to parts by weight, 50~1000 parts of fresh witloof；
Step 2 is 1000g according to solid-liquid ratio after sieving with 100 mesh sieve the chitin in step 1：10~20L adds after weighing
Enter in distilled water, stirs evenly and be placed under 90~100 DEG C of water bath with thermostatic control after 1~2h, be then transferred into high shear dispersion machine
Middle decentralized processing, is then transferred into enzymatic vessel, and 8~12% chitosan enzyme is added according to volume ratio, is stirred in heated constant temperature
Lower carry out enzyme digestion reaction increases the temperature to 100 DEG C after reaction, and Continuous Heat inactivates 15~30min, is then rapidly cooled to room
Temperature, centrifugation, obtained sediment are washed with distilled water 3~5 times, and chitosan is made in sediment drying；
Step 3 after cleaning the fresh witloof in step 1, is placed in 2~5min in boiling water, and taking-up is cooled to room temperature, slice
Afterwards, it is dipped into 10~60s in the ethanol solution dissolved with vitamin E, dry after taking-up, crushing, the coarse powder raw material obtained after sieving
According to material night than 1：10~1：20 add water mixing, are 4.0~5.0 with dilute hydrochloric acid adjustment pH value, are then transferred into high shear dispersion
Decentralized processing in machine, is then transferred into enzymatic vessel, and the neutral protease enzymolysis of 25mg/l~28mg/l is added, and crosses filter out later
Filter residue is removed, the first leaching liquor of inulin is obtained；
First leaching liquor of inulin made from step 3 is filtered by step 4 by Ultra filtration membrane equipment, filtered
Filtrate enters back into nanofiltration membrane separation plant and is filtered, the inulin concentrate obtained after filtered trapped fluid is evaporated under reduced pressure,
For use；
Step 5, configuration concentration be 0.15~0.3mol/l sodium acetate solution, then with acetic acid adjust pH value be 3~
4.5, chitosan prepared by step 2 is according to mass ratio 0.5~1：10 are added to sodium acetate solution, and mass concentration is then added and is
1.0% aspergillus niger, it is 2~4% to make enzyme bottom ratio, after stirring 10~30min, is transferred completely into high shear dispersion machine at dispersion
Reason increases the temperature to 100 DEG C after reaction, and Continuous Heat inactivates 15~30min, is then rapidly cooled to room temperature, is obtained by filtration
The first filtrate of chitosan oligosaccharide；
First filtrate of chitosan oligosaccharide made from step 5 is filtered by step 6 by Ultra filtration membrane equipment, filtered
Filtrate enters back into nanofiltration membrane separation plant and is filtered, and filtered trapped fluid is that soluble solid content is mass fraction 20
~25%, the chitosan oligosaccharide concentrate obtained after filtered trapped fluid is evaporated under reduced pressure, for use；
Step 7, by the inulin concentrate that step 5 obtains and the chitosan oligosaccharide concentrate that step 6 obtains after mixing, freezing
Dry, vacuum seal bag packaging is made with the complex polysaccharide electuary for adjusting intestinal flora function.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function of the present invention, height is cut in step 2
Cut 20~30min of rotating speed cutting decentralized processing that dispersion machine processing is 2500~3000r/min；Chitosan enzyme in enzyme digestion reaction
Enzyme activity degree be 200~300U/ml, 45~50 DEG C, 200~500rad/min of speed of agitator, 3~4h of reaction time of reaction temperature,
During enzyme digestion reaction pH7.4~7.6 are reconciled with sodium carbonate liquor；Machine 12000~20000rad/min of centrifuge speed, centrifugation
10~20min.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function of the present invention, ethyl alcohol in step 3
The content of vitamin E is 10~30g/l in solution；The rotating speed cutting point that the processing of high shear dispersion machine is 2500~3000r/min
Dissipate 20~30min of processing；Enzymatic hydrolysis condition is 70~75 DEG C of 1~2h of processing.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function of the present invention, is filtered in step 4
Trapped fluid afterwards is that soluble solid content is mass fraction 20~25%；The pressure of vacuum distillation be -100~-85kPa,
The temperature of vacuum distillation is 40~45 DEG C, the time is 20~60min；A concentration of soluble solid of the inulin concentrate
Content is mass fraction 35~40%.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function of the present invention, height is cut in step 5
It is temperature is 45~50 DEG C to cut dispersion machine processing, with 3~6h of rotating speed cutting decentralized processing of 2500~3000r/min.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function of the present invention, is depressurized in step 6
The pressure of distillation is -100~-85kPa, the temperature of vacuum distillation is 30~35 DEG C, the time is 20~60min；The shell is few
A concentration of soluble solid content of sugared concentrate is mass fraction 35~40%.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function of the present invention, freezes in step 7
Drying condition be at -20 DEG C pre-freeze 2h, be placed in vacuum freeze drier, be evacuated to 10~100Pa, cold hydrazine temperature
- 35~-40 DEG C of degree, 4~8h of cooling time.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function of the present invention, the ultrafiltration membrane
Molecular cut off is used to be detached for the rolling ultrafiltration membrane of 20~50KD, the rolling ultrafiltration membrane is by polystyrene or polyethers
Acidproof ultrafiltration membrane made of sulphone resin or Kynoar.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function of the present invention, the NF membrane
Molecular cut off is used to be detached for the rolling NF membrane of 300~1000Da, the rolling NF membrane of polyamide by being made
Acidproof NF membrane.
It is a kind of that there is the complex polysaccharide electuary for adjusting intestinal flora function, described having to adjust answering for intestinal flora function
Polysaccharide electuary is closed to be made of 50~1000 parts of chitin, 50~1000 parts of fresh witloof；Described having adjusts enterobacteriaceae
The complex polysaccharide electuary of group's function is made of following methods：
Step 1, the chitin for weighing 100~150 parts respectively according to parts by weight, 500~700 parts of fresh witloof；
Step 2 is 1000g according to solid-liquid ratio after sieving with 100 mesh sieve the chitin in step 1：10~20L adds after weighing
Enter in distilled water, stirs evenly and be placed under 90~100 DEG C of water bath with thermostatic control after 1~2h, be then transferred into high shear dispersion machine
Middle decentralized processing, is then transferred into enzymatic vessel, and 8~12% chitosan enzyme is added according to volume ratio, is stirred in heated constant temperature
Lower carry out enzyme digestion reaction increases the temperature to 100 DEG C after reaction, and Continuous Heat inactivates 15~30min, is then rapidly cooled to room
Temperature, centrifugation, obtained sediment are washed with distilled water 3~5 times, and chitosan is made in sediment drying；
Step 3 after cleaning the fresh witloof in step 1, is placed in 2~5min in boiling water, and taking-up is cooled to room temperature, slice
Afterwards, it is dipped into 10~60s in the ethanol solution dissolved with vitamin E, dry after taking-up, crushing, the coarse powder raw material obtained after sieving
According to material night than 1：10~1：20 add water mixing, are 4.0~5.0 with dilute hydrochloric acid adjustment pH value, are then transferred into high shear dispersion
Decentralized processing in machine, is then transferred into enzymatic vessel, and the neutral protease enzymolysis of 25mg/l~28mg/l is added, and crosses filter out later
Filter residue is removed, the first leaching liquor of inulin is obtained；
First leaching liquor of inulin made from step 3 is filtered by step 4 by Ultra filtration membrane equipment, filtered
Filtrate enters back into nanofiltration membrane separation plant and is filtered, the inulin concentrate obtained after filtered trapped fluid is evaporated under reduced pressure,
For use；
Step 5, configuration concentration be 0.15~0.3mol/l sodium acetate solution, then with acetic acid adjust pH value be 3~
4.5, chitosan prepared by step 2 is according to mass ratio 0.5~1：10 are added to sodium acetate solution, and mass concentration is then added and is
1.0% aspergillus niger, it is 2~4% to make enzyme bottom ratio, after stirring 10~30min, is transferred completely into high shear dispersion machine at dispersion
Reason increases the temperature to 100 DEG C after reaction, and Continuous Heat inactivates 15~30min, is then rapidly cooled to room temperature, is obtained by filtration
The first filtrate of chitosan oligosaccharide；
First filtrate of chitosan oligosaccharide made from step 5 is filtered by step 6 by Ultra filtration membrane equipment, filtered
Filtrate enters back into nanofiltration membrane separation plant and is filtered, and filtered trapped fluid is that soluble solid content is mass fraction 20
~25%, the chitosan oligosaccharide concentrate obtained after filtered trapped fluid is evaporated under reduced pressure, for use；
Step 7, by the inulin concentrate that step 5 obtains and the chitosan oligosaccharide concentrate that step 6 obtains after mixing, freezing
Dry, vacuum seal bag packaging is made with the complex polysaccharide electuary for adjusting intestinal flora function.
It is of the present invention that there is the complex polysaccharide electuary for adjusting intestinal flora function, the wherein quality of chitosan oligosaccharide and inulin
Than being 1~20：1~20.
It is of the present invention that there is the complex polysaccharide electuary for adjusting intestinal flora function, the wherein quality of chitosan oligosaccharide and inulin
Than being 1：2、1：4、1：7、1：10、1：15、1：20.
It is of the present invention that there is the complex polysaccharide electuary for adjusting intestinal flora function, the wherein quality of chitosan oligosaccharide and inulin
Than being 2：1、4：1、7：1、10：1、15：1、20：1.
Advantageous effect：
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function of the present invention, the preparation of inulin are adopted
With decentralized processing in high shear dispersion machine, the utilization of high shear dispersion machine can improve the dissolubility of inulin, improve recovery rate, drop
Low dissolution time prevents the Starch Conversion in witloof at small dextrins, reduces the impurity content in inulin, in favor of reducing
Subsequent purification technology difficulty, and low energy consumption, and using the extraction conditions of pH value 4.0-4.5, but protein is due to equal electro-deposition
Solubility is reduced, to reduce the dissolution of the protein in raw material, the impurity level in extracting solution is reduced, later using ultrafiltration membrane point
It is filtered from equipment, filtered filtrate enters back into nanofiltration membrane separation plant and is filtered, and avoids conventional milk of lime and removes
Live together reason, technique simplifies, and makes reaction condition in the soda acid range of relatively low temperature and mitigation, reduce equipment and it is related at
This, reduces pollution caused by energy consumption and soda acid.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function of the present invention, the system of chitosan oligosaccharide
It is standby；Aspergillus niger is utilized, enzyme digestion reaction, enzyme digestion reaction decentralized processing in high shear dispersion machine, high shear point are carried out to chitosan
The dissolubility of chitosan oligosaccharide can be improved by dissipating the utilization of machine, improve enzymatic hydrolyzation, reduce enzymolysis time, and enzymolysis process and degradation production
The distribution of object relative molecular weight is easier to control, and the chitosan biological activity of preparation is high, and product process is easy to control, and not to ring
Border pollutes, and is filtered later using Ultra filtration membrane equipment after Enzymatic Hydrolysis of Chitosan, filtered filtrate enters back into nanofiltration
Membrane separation plant is filtered, and improves the purity of product, the molecular weight of chitosan oligosaccharide<3000, deacetylation>92%, and two
Equipment used in kind method is identical, can reduce equipment and relevant cost.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function of the present invention, the inulin carry
The rate highest of taking can reach 91.5%, and inulin purity reaches 96.6%, and inulin crystal is directly from molten containing pigment and salt ion
It is precipitated in liquid, inulin product purity is high, and decoloration desalting effect is good.
It is of the present invention that there is the complex polysaccharide electuary for adjusting intestinal flora function, it is proved by contrast test, it is described
With adjust intestinal flora function complex polysaccharide electuary compared to single chitosan or inulin with more superior adjusting
The effect of intestinal microflora illustrates chitosan and chrysanthemum in the complex polysaccharide electuary with adjusting intestinal flora function
Powder has good synergistic function.
It is of the present invention that there is the complex polysaccharide electuary for adjusting intestinal flora function, by taking front and back contrast test
It proves, has taken chitosan oligosaccharide control group, inulin control group, the complex polysaccharide electuary phase with adjusting intestinal flora function
Before not taking, after test-meal four weeks, body fat weight and body fat percentage are substantially reduced, and on probation described
That there is in the complex polysaccharide electuary for adjusting intestinal flora function fat content to reduce is the most apparent, illustrate that described having is adjusted
The complex polysaccharide electuary for saving intestinal flora function has remarkable result to adjusting body fat, can prevent and treat obesity and adjust
The intestinal disorder caused by obesity is saved, and there is apparent synergistic effect between complex polysaccharide.
Of the present invention to have the complex polysaccharide electuary for adjusting intestinal flora function, chitosan and inulin are according to a certain ratio
Combination has good adjustment effect to enteric flora disturbance caused by many reasons, and of the present invention with adjusting
The complex polysaccharide electuary of intestinal flora function has good effect to intestinal disorder caused by adjusting obesity.
It is of the present invention that there is the complex polysaccharide electuary for adjusting intestinal flora function, it daily can make an addition to food, medicine
Product have the function of health care on the basis of improving mouthfeel.
Specific implementation mode
Specific implementation mode one：
A kind of preparation method with the complex polysaccharide electuary for adjusting intestinal flora function, includes the following steps：
Step 1, the chitin for weighing 100 parts respectively according to parts by weight, 500 parts of fresh witloof；
Step 2 is 1000g according to solid-liquid ratio after sieving with 100 mesh sieve the chitin in step 1：10L is added after weighing to be steamed
In distilled water, stirs evenly and be placed under 90 DEG C of water bath with thermostatic control after 1h, be then transferred into decentralized processing in high shear dispersion machine, so
After be transferred in enzymatic vessel, according to volume ratio be added 8% chitosan enzyme, heated constant temperature stirring under carry out enzyme digestion reaction, instead
100 DEG C are increased the temperature to after answering, Continuous Heat inactivates 15min, is then rapidly cooled to room temperature, centrifuges, obtained sediment
It is washed with distilled water 3 times, chitosan is made in sediment drying；
Step 3 after cleaning the fresh witloof in step 1, is placed in 2min in boiling water, and taking-up is cooled to room temperature, after slice,
It is dipped into 10s in the ethanol solution dissolved with vitamin E, dry after taking-up, crushing, the coarse powder raw material obtained after sieving is according to material night
Than 1：10 add water mixing, are 4.0 with dilute hydrochloric acid adjustment pH value, are then transferred into decentralized processing in high shear dispersion machine, then turn
It moves on in enzymatic vessel, the neutral protease enzymolysis of 25mg/l is added, is filtered to remove filter residue later, obtain the first leaching liquor of inulin；
First leaching liquor of inulin made from step 3 is filtered by step 4 by Ultra filtration membrane equipment, filtered
Filtrate enters back into nanofiltration membrane separation plant and is filtered, the inulin concentrate obtained after filtered trapped fluid is evaporated under reduced pressure,
For use；
Step 5, the sodium acetate solution that configuration concentration is 0.15mol/l, it is 3 then to adjust pH value with acetic acid, and step 2 is made
Standby chitosan is according to mass ratio 0.5：10 are added to sodium acetate solution, and the aspergillus niger that mass concentration is 1.0% is then added, makes
Enzyme bottom ratio is 2%, after stirring 10min, is transferred completely into decentralized processing in high shear dispersion machine, increases the temperature to after reaction
100 DEG C, Continuous Heat inactivates 15min, is then rapidly cooled to room temperature, the first filtrate of chitosan oligosaccharide is obtained by filtration；
First filtrate of chitosan oligosaccharide made from step 5 is filtered by step 6 by Ultra filtration membrane equipment, filtered
Filtrate enters back into nanofiltration membrane separation plant and is filtered, and filtered trapped fluid is that soluble solid content is mass fraction
20%, the chitosan oligosaccharide concentrate obtained after filtered trapped fluid is evaporated under reduced pressure, for use；
Step 7, by the inulin concentrate that step 5 obtains and the chitosan oligosaccharide concentrate that step 6 obtains after mixing, freezing
Dry, vacuum seal bag packaging is made with the complex polysaccharide electuary for adjusting intestinal flora function.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, in step 2
The rotating speed cutting decentralized processing 20min that the processing of high shear dispersion machine is 2500r/min；The enzyme activity degree of chitosan enzyme in enzyme digestion reaction
For 200U/ml, 45 DEG C, speed of agitator 200rad/min, reaction time 3h of reaction temperature, enzyme digestion reaction uses sodium carbonate molten in the process
Liquid reconciles pH7.4；Machine centrifuge speed 12000rad/min centrifuges 10min.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, in step 3
The content of vitamin E is 10g/l in ethanol solution；The rotating speed cutting decentralized processing that the processing of high shear dispersion machine is 2500r/min
30min；Enzymatic hydrolysis condition is 70 DEG C of processing 1h.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, in step 4
Filtered trapped fluid is that soluble solid content is mass fraction 20%；The pressure of vacuum distillation is -100kPa, decompression is steamed
The temperature evaporated is 40 DEG C, time 20min；A concentration of soluble solid content of the inulin concentrate is mass fraction
35%.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, in step 5
It is 45 DEG C that the processing of high shear dispersion machine, which is temperature, with the rotating speed cutting decentralized processing 3h of 2500r/min.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, in step 6
The pressure of vacuum distillation is -100kPa, the temperature of vacuum distillation is 30 DEG C, time 20min；The chitosan oligosaccharide concentrate
A concentration of soluble solid content is mass fraction 35%.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, in step 7
Freeze-drying condition be at -20 DEG C pre-freeze 2h, be placed in vacuum freeze drier, be evacuated to 10Pa, cold hydrazine temperature -
35 DEG C, cooling time 4h.
There is tool prepared by the preparation method for the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment
There is the complex polysaccharide electuary for adjusting intestinal flora function, the mass ratio of chitosan oligosaccharide and inulin is 1：10.
There is tool prepared by the preparation method for the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment
There is the complex polysaccharide electuary for adjusting intestinal flora function, carries out the contrast test that obesity mice intestinal flora composition influences, choose
50 special male mices of experiment, 20 ± 2g of mouse weight are randomly divided into 5 groups, every group 10, respectively blank group, model pair
According to group, positive chitosan oligosaccharide group, positive inulin group, present embodiment group.Blank group gives normal diet, remaining four groups give it is high in fat
After fat forage feed 8 weeks, positive chitosan oligosaccharide group, positive inulin group, present embodiment group animal continuously give respectively chitosan oligosaccharide,
Inulin, with the complex polysaccharide electuary gavage 4 weeks for adjusting intestinal flora function, blank group and model control group give isometric(al)
Physiological saline, measure administration before, administration after each mouse weight, take stool in mice under aseptic condition at the end of experiment,
Sequencing analysis is carried out to stool in mice enterobacteriaceae using 16S-rRNA, the situation of change that intestinal flora forms between comparative group, mouse
Changes of weight is as shown in table 1, and the relative abundance of the Pseudomonas with significant difference is as shown in table 2：
1 mouse weight of table changes contrast table
Note：The * P compared with blank control group<0.05；The * P compared with model control group<0.05.
By table 1 can, it is evident that each fat group mouse original body mass and no significant difference, when give chitosan oligosaccharide,
After 4 weeks, mouse weight has compared to model control group for inulin, the complex polysaccharide electuary gavage with adjusting intestinal flora function
It is decreased obviously, but present embodiment has the complex polysaccharide electuary effect for adjusting intestinal flora function the most notable, almost can
It is reduced to naive mice weight and reaches same standard, restore the weight of standard.
The relative abundance contrast table of 2 Pseudomonas of table
Note：The * P compared with blank control group<0.05；The * P compared with model control group<0.05.
, it can be seen that significantly raised, Bacteroidetes hair occurs for the Firmicutes of model control group and Proteobacteria from table 2
Life is substantially reduced, and gives chitosan oligosaccharide, inulin, the complex polysaccharide electuary gavage with adjusting intestinal flora function after 4 weeks, bacteroid
Door level dramatically increases, and Firmicutes and Proteobacteria level significantly reduce, and with the compound of adjusting intestinal flora function
The Pseudomonas abundance of polysaccharide electuary and blank group is closest, illustrates the complex polysaccharide electuary with adjusting intestinal flora function to height
The mouse intestinal flora imbalance of fat diet induced has adjustment effect, and with the complex polysaccharide punching for adjusting intestinal flora function
Agent has preferably readjustment effect than single chitosan oligosaccharide, inulin, illustrates with the complex polysaccharide punching for adjusting intestinal flora function
Agent has played apparent synergistic effect.
Specific implementation mode two：
A kind of preparation method with the complex polysaccharide electuary for adjusting intestinal flora function, includes the following steps：
Step 1, the chitin for weighing 700 parts respectively according to parts by weight, 50 parts of fresh witloof；
Step 2 is 1000g according to solid-liquid ratio after sieving with 100 mesh sieve the chitin in step 1：20L is added after weighing to be steamed
In distilled water, stirs evenly and is placed under 100 DEG C of water bath with thermostatic control after 1h, be then transferred into decentralized processing in high shear dispersion machine,
It is then transferred into enzymatic vessel, 12% chitosan enzyme is added according to volume ratio, enzyme digestion reaction is carried out under heated constant temperature stirring,
100 DEG C are increased the temperature to after reaction, and Continuous Heat inactivates 30min, is then rapidly cooled to room temperature, centrifuges, obtained precipitation
Object is washed with distilled water 5 times, and chitosan is made in sediment drying；
Step 3 after cleaning the fresh witloof in step 1, is placed in 5min in boiling water, and taking-up is cooled to room temperature, after slice,
It is dipped into 60s in the ethanol solution dissolved with vitamin E, dry after taking-up, crushing, the coarse powder raw material obtained after sieving is according to material night
Than 1：20 add water mixing, are 5.0 with dilute hydrochloric acid adjustment pH value, are then transferred into decentralized processing in high shear dispersion machine, then turn
It moves on in enzymatic vessel, the neutral protease enzymolysis of 28mg/l is added, is filtered to remove filter residue later, obtain the first leaching liquor of inulin；
First leaching liquor of inulin made from step 3 is filtered by step 4 by Ultra filtration membrane equipment, filtered
Filtrate enters back into nanofiltration membrane separation plant and is filtered, the inulin concentrate obtained after filtered trapped fluid is evaporated under reduced pressure,
For use；
Step 5, the sodium acetate solution that configuration concentration is 0.3mol/l, it is 4.5 then to adjust pH value with acetic acid, by step 2
The chitosan of preparation is according to quality 1：10 are added to sodium acetate solution, and the aspergillus niger that mass concentration is 1.0% is then added, makes enzyme
Bottom ratio is 4%, after stirring 30min, is transferred completely into decentralized processing in high shear dispersion machine, increases the temperature to after reaction
100 DEG C, Continuous Heat inactivates 30min, is then rapidly cooled to room temperature, the first filtrate of chitosan oligosaccharide is obtained by filtration；
First filtrate of chitosan oligosaccharide made from step 5 is filtered by step 6 by Ultra filtration membrane equipment, filtered
Filtrate enters back into nanofiltration membrane separation plant and is filtered, and filtered trapped fluid is that soluble solid content is mass fraction
25%, the chitosan oligosaccharide concentrate obtained after filtered trapped fluid is evaporated under reduced pressure, for use；
Step 7, by the inulin concentrate that step 5 obtains and the chitosan oligosaccharide concentrate that step 6 obtains after mixing, freezing
Dry, vacuum seal bag packaging is made with the complex polysaccharide electuary for adjusting intestinal flora function.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, in step 2
The rotating speed cutting decentralized processing 30min that the processing of high shear dispersion machine is 3000r/min；The enzyme activity degree of chitosan enzyme in enzyme digestion reaction
For 300U/ml, 50 DEG C, speed of agitator 500rad/min, reaction time 4h of reaction temperature, enzyme digestion reaction uses sodium carbonate molten in the process
Liquid reconciles pH7.6；Machine centrifuge speed 20000rad/min centrifuges 20min.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, in step 3
The content of vitamin E is 30g/l in ethanol solution；The rotating speed cutting decentralized processing that the processing of high shear dispersion machine is 3000r/min
30min；Enzymatic hydrolysis condition is 75 DEG C of processing 2h.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, in step 4
Filtered trapped fluid is that soluble solid content is mass fraction 25%；The pressure of vacuum distillation is -85kPa, decompression is steamed
The temperature evaporated is 45 DEG C, time 60min；A concentration of soluble solid content of the inulin concentrate is mass fraction
40%.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, in step 5
It is 50 DEG C that the processing of high shear dispersion machine, which is temperature, with the rotating speed cutting decentralized processing 6h of 3000r/min.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, in step 6
The pressure of vacuum distillation is -85kPa, the temperature of vacuum distillation is 35 DEG C, time 60min；The chitosan oligosaccharide concentrate
A concentration of soluble solid content is mass fraction 40%.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, in step 7
Freeze-drying condition be at -20 DEG C pre-freeze 2h, be placed in vacuum freeze drier, be evacuated to 100Pa, cold hydrazine temperature
- 40 DEG C of degree, cooling time 8h.
There is tool prepared by the preparation method for the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment
There is the complex polysaccharide electuary for adjusting intestinal flora function, the mass ratio of chitosan oligosaccharide and inulin is 7：1.
There is tool prepared by the preparation method for the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment
There is the complex polysaccharide electuary for adjusting intestinal flora function, according to the Ministry of Public Health《Health food is examined and assessment technique specification 2003》's
Intestinal flora function human experiment experimental evaluation method is adjusted, to chitosan oligosaccharide, inulin, with the compound of adjusting intestinal flora function
Polysaccharide electuary is evaluated,
160 volunteers carry out measure of merit, and 160 volunteers are 80 males, 80 women, every group of 40 people, and random 4
Group be model control group, chitosan oligosaccharide control group, inulin control group, present embodiment group, it is sterile to adopt before subject's test-meal
Subject's excrement, 16s rDNA sequencings is taken to examine intestinal flora, the reference as background level.Chitosan oligosaccharide control group is taken daily
Chitosan oligosaccharide 6g, inulin control group take inulin 6g, present embodiment group and accordingly take 6g daily with adjusting intestinal flora daily
The complex polysaccharide electuary of function is continuously taken 4 weeks, and model control group takes the physiological saline of respective volume.After 4 weeks, take by
Examination person's excrement, 16s rDNA sequencings are examined intestinal flora to compare the increased multiple of its relative abundance compared with background level, are tied
Fruit is as shown in table 3：
The tested intestinal flora sequencing abundance comparison of 3 human body of table
Note：The * P compared with model control group<0.05；
From, it can be seen that compared with before edible, having taken chitosan oligosaccharide control group, inulin control group, present embodiment in table 3
Group is relative to model control group, after test-meal four weeks, Bifidobacterium.Butyric acid vibrios, lactobacillus, galactococcus, Ross formula bacterium, excrement bar of dwelling
Bacterium, Eubacterium are significantly increased, and have the complex polysaccharide for adjusting intestinal flora function described in present embodiment on probation
Electuary is accelerated the most obviously, illustrates there is the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment
There is remarkable result to adjusting intestinal flora, there is apparent synergistic effect between complex polysaccharide.
Specific implementation mode three：
A kind of preparation method with the complex polysaccharide electuary for adjusting intestinal flora function, includes the following steps：
Step 1, the chitin for weighing 500 parts respectively according to parts by weight, 500 parts of fresh witloof；
Step 2 is 1000g according to solid-liquid ratio after sieving with 100 mesh sieve the chitin in step 1：15L is added after weighing to be steamed
In distilled water, stirs evenly and is placed under 100 DEG C of water bath with thermostatic control after 1h, be then transferred into decentralized processing in high shear dispersion machine,
It is then transferred into enzymatic vessel, 10% chitosan enzyme is added according to volume ratio, enzyme digestion reaction is carried out under heated constant temperature stirring,
100 DEG C are increased the temperature to after reaction, and Continuous Heat inactivates 20min, is then rapidly cooled to room temperature, centrifuges, obtained precipitation
Object is washed with distilled water 4 times, and chitosan is made in sediment drying；
Step 3 after cleaning the fresh witloof in step 1, is placed in 3min in boiling water, and taking-up is cooled to room temperature, after slice,
It is dipped into 30s in the ethanol solution dissolved with vitamin E, dry after taking-up, crushing, the coarse powder raw material obtained after sieving is according to material night
Than 1：15 add water mixing, are 4.0 with dilute hydrochloric acid adjustment pH value, are then transferred into decentralized processing in high shear dispersion machine, then turn
It moves on in enzymatic vessel, the neutral protease enzymolysis of 28mg/l is added, is filtered to remove filter residue later, obtain the first leaching liquor of inulin；
First leaching liquor of inulin made from step 3 is filtered by step 4 by Ultra filtration membrane equipment, filtered
Filtrate enters back into nanofiltration membrane separation plant and is filtered, the inulin concentrate obtained after filtered trapped fluid is evaporated under reduced pressure,
For use；
Step 5, the sodium acetate solution that configuration concentration is 0.2mol/l, it is 4 then to adjust pH value with acetic acid, and step 2 is made
Standby chitosan is according to mass ratio 1：10 are added to sodium acetate solution, and the aspergillus niger that mass concentration is 1.0% is then added, makes enzyme
Bottom ratio is 3%, after stirring 20min, is transferred completely into decentralized processing in high shear dispersion machine, increases the temperature to after reaction
100 DEG C, Continuous Heat inactivates 15min, is then rapidly cooled to room temperature, the first filtrate of chitosan oligosaccharide is obtained by filtration；
First filtrate of chitosan oligosaccharide made from step 5 is filtered by step 6 by Ultra filtration membrane equipment, filtered
Filtrate enters back into nanofiltration membrane separation plant and is filtered, and filtered trapped fluid is that soluble solid content is mass fraction
25%, the chitosan oligosaccharide concentrate obtained after filtered trapped fluid is evaporated under reduced pressure, for use；
Step 7, by the inulin concentrate that step 5 obtains and the chitosan oligosaccharide concentrate that step 6 obtains after mixing, freezing
Dry, vacuum seal bag packaging is made with the complex polysaccharide electuary for adjusting intestinal flora function.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, in step 2
The rotating speed cutting decentralized processing 20min that the processing of high shear dispersion machine is 3000r/min；The enzyme activity degree of chitosan enzyme in enzyme digestion reaction
For 200U/ml, 45 DEG C, speed of agitator 300rad/min, reaction time 4h of reaction temperature, enzyme digestion reaction uses sodium carbonate molten in the process
Liquid reconciles pH7.5；Machine centrifuge speed 15000rad/min centrifuges 15min.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, in step 3
The content of vitamin E is 20g/l in ethanol solution；The rotating speed cutting decentralized processing that the processing of high shear dispersion machine is 3000r/min
30min；Enzymatic hydrolysis condition is 75 DEG C of processing 2h.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, in step 4
Filtered trapped fluid is that soluble solid content is mass fraction 25%；The pressure of vacuum distillation is -85kPa, decompression is steamed
The temperature evaporated is 45 DEG C, time 30min；A concentration of soluble solid content of the inulin concentrate is mass fraction
40%.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, in step 5
It is 50 DEG C that the processing of high shear dispersion machine, which is temperature, with the rotating speed cutting decentralized processing 5h of 3000r/min.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, in step 6
The pressure of vacuum distillation is -85kPa, the temperature of vacuum distillation is 35 DEG C, time 30min；The chitosan oligosaccharide concentrate
A concentration of soluble solid content is mass fraction 40%.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, in step 7
Freeze-drying condition be at -20 DEG C pre-freeze 2h, be placed in vacuum freeze drier, be evacuated to 100Pa, cold hydrazine temperature
- 40 DEG C of degree, cooling time 5h.
There is tool prepared by the preparation method for the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment
There is the complex polysaccharide electuary for adjusting intestinal flora function, the mass ratio of chitosan oligosaccharide and inulin is 1：2.
There is tool prepared by the preparation method for the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment
There is the complex polysaccharide electuary for adjusting intestinal flora function, designed using own control, to chitosan oligosaccharide, inulin, there is adjusting enteron aisle
The complex polysaccharide electuary of flora function carries out human body indicators evaluation, and 120 volunteers carry out measure of merit, and 120 is equal for volunteer
It is more than the man of 80kg for weight, is randomly divided into 3 groups as chitosan oligosaccharide control group, inulin control group, present embodiment group.Chitosan oligosaccharide
Control group takes chitosan oligosaccharide 6g daily, inulin control group takes inulin 6g daily, present embodiment group accordingly takes 6g tools daily
There is the complex polysaccharide electuary for adjusting intestinal flora function, continuously takes 4 weeks.During test-meal diet and amount of exercise with before experiment
Be consistent, indices in test-meal start and at the end of respectively test it is primary.Weight test result is as shown in table 4, body fat
Total amount and fat percentage test result are as shown in table 5：
4 weight test comparison table of table (kg,)
Note：The * P compared with before test-meal<0.05；
From, it can be seen that compared with taking before edible, having taken chitosan oligosaccharide control group, inulin control group, this implementation in table 4
Before mode group is not relative to taking, after test-meal four weeks, weight is substantially reduced, and having described in present embodiment on probation
The complex polysaccharide electuary weight loss for adjusting intestinal flora function is the most apparent, illustrates that having described in present embodiment is adjusted
The complex polysaccharide electuary of intestinal flora function has remarkable result to adjusting body fat, can prevent and treat obesity and adjust
The intestinal disorder caused by obesity, and there is apparent synergistic effect between complex polysaccharide.
5 body fat total amount of table and fat percentage test comparison table
Note：The * P compared with before test-meal<0.05；
From, it can be seen that compared with taking before edible, having taken chitosan oligosaccharide control group, inulin control group, this implementation in table 5
Before mode group is not relative to taking, after test-meal four weeks, body fat weight and body fat percentage are substantially reduced, and
Have fat content reduction in the complex polysaccharide electuary for adjusting intestinal flora function the most apparent described in present embodiment on probation,
Illustrate the complex polysaccharide electuary with adjusting intestinal flora function described in present embodiment to adjusting body fat with aobvious
Effect is write, can prevent and treat fat and adjusts the intestinal disorder caused by obesity, and is had between complex polysaccharide bright
Aobvious synergistic effect.
Specific implementation mode four：
A kind of preparation method with the complex polysaccharide electuary for adjusting intestinal flora function, includes the following steps：
Step 1, the chitin for weighing 100~150 parts respectively according to parts by weight, 500~700 parts of fresh witloof；
Step 2 is 1000g according to solid-liquid ratio after sieving with 100 mesh sieve the chitin in step 1：10~20L adds after weighing
Enter in distilled water, stirs evenly and be placed under 90~100 DEG C of water bath with thermostatic control after 1~2h, be then transferred into high shear dispersion machine
Middle decentralized processing, is then transferred into enzymatic vessel, and 8~12% chitosan enzyme is added according to volume ratio, is stirred in heated constant temperature
Lower carry out enzyme digestion reaction increases the temperature to 100 DEG C after reaction, and Continuous Heat inactivates 15~30min, is then rapidly cooled to room
Temperature, centrifugation, obtained sediment are washed with distilled water 3~5 times, and chitosan is made in sediment drying；
Step 3 after cleaning the fresh witloof in step 1, is placed in 2~5min in boiling water, and taking-up is cooled to room temperature, slice
Afterwards, it is dipped into 10~60s in the ethanol solution dissolved with vitamin E, dry after taking-up, crushing, the coarse powder raw material obtained after sieving
According to material night than 1：10~1：20 add water mixing, are 4.0~5.0 with dilute hydrochloric acid adjustment pH value, are then transferred into high shear dispersion
Decentralized processing in machine, is then transferred into enzymatic vessel, and the neutral protease enzymolysis of 25mg/l~28mg/l is added, and crosses filter out later
Filter residue is removed, the first leaching liquor of inulin is obtained；
First leaching liquor of inulin made from step 3 is filtered by step 4 by Ultra filtration membrane equipment, filtered
Filtrate enters back into nanofiltration membrane separation plant and is filtered, the inulin concentrate obtained after filtered trapped fluid is evaporated under reduced pressure,
For use；
Step 5, configuration concentration be 0.15~0.3mol/l sodium acetate solution, then with acetic acid adjust pH value be 3~
4.5, chitosan prepared by step 2 is according to mass ratio 0.5~1：10 are added to sodium acetate solution, and mass concentration is then added and is
1.0% aspergillus niger, it is 2~4% to make enzyme bottom ratio, after stirring 10~30min, is transferred completely into high shear dispersion machine at dispersion
Reason increases the temperature to 100 DEG C after reaction, and Continuous Heat inactivates 15~30min, is then rapidly cooled to room temperature, is obtained by filtration
The first filtrate of chitosan oligosaccharide；
First filtrate of chitosan oligosaccharide made from step 5 is filtered by step 6 by Ultra filtration membrane equipment, filtered
Filtrate enters back into nanofiltration membrane separation plant and is filtered, and filtered trapped fluid is that soluble solid content is mass fraction 20
~25%, the chitosan oligosaccharide concentrate obtained after filtered trapped fluid is evaporated under reduced pressure, for use；
Step 7, by the inulin concentrate that step 5 obtains and the chitosan oligosaccharide concentrate that step 6 obtains after mixing, freezing
Dry, vacuum seal bag packaging is made with the complex polysaccharide electuary for adjusting intestinal flora function.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, the system of inulin
Standby using decentralized processing in high shear dispersion machine, the utilization of high shear dispersion machine can improve the dissolubility of inulin, improve extraction
Rate reduces dissolution time, prevents the Starch Conversion in witloof at small dextrins, the impurity content in inulin is reduced, with profit
In reduction subsequent purification technology difficulty, and low energy consumption, and using the extraction conditions of pH value 4.0-4.5, but protein is because of equal electricity
It deposits and reduces solubility, to reduce the dissolution of the protein in raw material, the impurity level in extracting solution is reduced, later using super
Filter membrane separation equipment is filtered, and filtered filtrate enters back into nanofiltration membrane separation plant and is filtered, and avoids conventional stone
Grey breast removal of impurities processing, technique simplify, make reaction condition in the soda acid range of relatively low temperature and mitigation, reduce equipment and phase
Cost is closed, pollution caused by energy consumption and soda acid is reduced.
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, chitosan oligosaccharide
It prepares；Aspergillus niger is utilized, enzyme digestion reaction, enzyme digestion reaction decentralized processing, high shear in high shear dispersion machine are carried out to chitosan
The utilization of dispersion machine can improve the dissolubility of chitosan oligosaccharide, improve enzymatic hydrolyzation, reduce enzymolysis time, and enzymolysis process and degradation
The distribution of product relative molecular weight is easier to control, and the chitosan biological activity of preparation is high, and product process is easy to control, and not right
Environment pollutes, and is filtered later using Ultra filtration membrane equipment after Enzymatic Hydrolysis of Chitosan, filtered filtrate, which enters back into, to be received
Filter membrane separation equipment is filtered, and improves the purity of product, the molecular weight of chitosan oligosaccharide<3000, deacetylation>92%, and
Equipment used in two methods is identical, can reduce equipment and relevant cost.
The preparation method preparation of the complex polysaccharide electuary with adjusting intestinal flora function described in present embodiment is answered
Polysaccharide electuary is closed, chitosan combines according to a certain ratio with inulin, has to enteric flora disturbance caused by many reasons good
Adjustment effect, and the complex polysaccharide electuary with adjusting intestinal flora function of the present invention, caused by adjusting obesity
Intestinal disorder has good effect.
Preparation method of the present invention with the complex polysaccharide electuary for adjusting intestinal flora function prepares compound more
Sugared electuary daily can make an addition to food, drug, have the function of health care on the basis of improving mouthfeel.
Specific implementation mode five：
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function according to specific implementation mode four,
20~30min of rotating speed cutting decentralized processing that the processing of high shear dispersion machine is 2500~3000r/min in step 2；Enzyme digestion reaction
The enzyme activity degree of middle chitosan enzyme be 200~300U/ml, 45~50 DEG C, 200~500rad/min of speed of agitator of reaction temperature, instead
3~4h between seasonable, enzyme digestion reaction reconcile pH7.4~7.6 with sodium carbonate liquor in the process；Machine centrifuge speed 12000~
20000rad/min centrifuges 10~20min.
Specific implementation mode six：
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function according to specific implementation mode four,
The content of vitamin E is 10~30g/l in ethanol solution in step 3；The processing of high shear dispersion machine is 2500~3000r/min's
20~30min of rotating speed cutting decentralized processing；Enzymatic hydrolysis condition is 70~75 DEG C of 1~2h of processing.
Specific implementation mode seven：
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function according to specific implementation mode four,
It is mass fraction 20~25% that filtered trapped fluid, which is soluble solid content, in step 4；The pressure of vacuum distillation be-
100~-85kPa, vacuum distillation temperature be 40~45 DEG C, the time is 20~60min；The inulin concentrate it is a concentration of
Soluble solid content is mass fraction 35~40%.
Specific implementation mode eight：
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function according to specific implementation mode four,
It is 45~50 DEG C that the processing of high shear dispersion machine, which is temperature, in step 5, with the rotating speed cutting decentralized processing 3 of 2500~3000r/min
~6h.
Specific implementation mode nine：
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function according to specific implementation mode four,
The pressure being evaporated under reduced pressure in step 6 is -100~-85kPa, the temperature of vacuum distillation is 30~35 DEG C, the time is 20~60min；
A concentration of soluble solid content of the chitosan oligosaccharide concentrate is mass fraction 35~40%.
Specific implementation mode ten：
The preparation method with the complex polysaccharide electuary for adjusting intestinal flora function according to specific implementation mode four,
It is the pre-freeze 2h at -20 DEG C that condition is freeze-dried in step 7, be placed in vacuum freeze drier, be evacuated to 10~
100Pa, -35~-40 DEG C of cold hydrazine temperature, 4~8h of cooling time.
Specific implementation mode 11：
It is a kind of that there is the complex polysaccharide electuary for adjusting intestinal flora function, described having to adjust answering for intestinal flora function
Polysaccharide electuary is closed to be made of 50~1000 parts of chitin, 50~1000 parts of fresh witloof；Described having adjusts enterobacteriaceae
The complex polysaccharide electuary of group's function is made of following methods：
Step 1, the chitin for weighing 50~1000 parts respectively according to parts by weight, 50~1000 parts of fresh witloof；
Step 2 is 1000g according to solid-liquid ratio after sieving with 100 mesh sieve the chitin in step 1：10~20L adds after weighing
Enter in distilled water, stirs evenly and be placed under 90~100 DEG C of water bath with thermostatic control after 1~2h, be then transferred into high shear dispersion machine
Middle decentralized processing, is then transferred into enzymatic vessel, and 8~12% chitosan enzyme is added according to volume ratio, is stirred in heated constant temperature
Lower carry out enzyme digestion reaction increases the temperature to 100 DEG C after reaction, and Continuous Heat inactivates 15~30min, is then rapidly cooled to room
Temperature, centrifugation, obtained sediment are washed with distilled water 3~5 times, and chitosan is made in sediment drying；
Step 3 after cleaning the fresh witloof in step 1, is placed in 2~5min in boiling water, and taking-up is cooled to room temperature, slice
Afterwards, it is dipped into 10~60s in the ethanol solution dissolved with vitamin E, dry after taking-up, crushing, the coarse powder raw material obtained after sieving
According to material night than 1：10~1：20 add water mixing, are 4.0~5.0 with dilute hydrochloric acid adjustment pH value, are then transferred into high shear dispersion
Decentralized processing in machine, is then transferred into enzymatic vessel, and the neutral protease enzymolysis of 25mg/l~28mg/l is added, and crosses filter out later
Filter residue is removed, the first leaching liquor of inulin is obtained；
First leaching liquor of inulin made from step 3 is filtered by step 4 by Ultra filtration membrane equipment, filtered
Filtrate enters back into nanofiltration membrane separation plant and is filtered, the inulin concentrate obtained after filtered trapped fluid is evaporated under reduced pressure,
For use；
Step 5, configuration concentration be 0.15~0.3mol/l sodium acetate solution, then with acetic acid adjust pH value be 3~
4.5, chitosan prepared by step 2 is according to mass ratio 0.5~1：10 are added to sodium acetate solution, and mass concentration is then added and is
1.0% aspergillus niger, it is 2~4% to make enzyme bottom ratio, after stirring 10~30min, is transferred completely into high shear dispersion machine at dispersion
Reason increases the temperature to 100 DEG C after reaction, and Continuous Heat inactivates 15~30min, is then rapidly cooled to room temperature, is obtained by filtration
The first filtrate of chitosan oligosaccharide；
First filtrate of chitosan oligosaccharide made from step 5 is filtered by step 6 by Ultra filtration membrane equipment, filtered
Filtrate enters back into nanofiltration membrane separation plant and is filtered, and filtered trapped fluid is that soluble solid content is mass fraction 20
~25%, the chitosan oligosaccharide concentrate obtained after filtered trapped fluid is evaporated under reduced pressure, for use；
Step 7, by the inulin concentrate that step 5 obtains and the chitosan oligosaccharide concentrate that step 6 obtains after mixing, freezing
Dry, vacuum seal bag packaging is made with the complex polysaccharide electuary for adjusting intestinal flora function.
There is the complex polysaccharide electuary for adjusting intestinal flora function described in present embodiment, by taking front and back comparison
Experiment proves, has taken chitosan oligosaccharide control group, inulin control group, the complex polysaccharide punching with adjusting intestinal flora function
Before agent is not relative to taking, after test-meal four weeks, body fat weight and body fat percentage are substantially reduced, and on probation
Fat content reduces the most apparent in the complex polysaccharide electuary for having adjusting intestinal flora function, illustrates the tool
There is the complex polysaccharide electuary for adjusting intestinal flora function that there is remarkable result to adjusting body fat, obesity can be prevented and treated
And the intestinal disorder caused by obesity is adjusted, and there is apparent synergistic effect between complex polysaccharide.
Claims (8)
1. a kind of preparation method with the complex polysaccharide electuary for adjusting intestinal flora function, it is characterised in that：Including walking as follows
Suddenly：
Step 1, the chitin for weighing 50~1000 parts respectively according to parts by weight, 50~1000 parts of fresh witloof；
Step 2 is 1000g according to solid-liquid ratio after sieving with 100 mesh sieve the chitin in step 1：10~20L is added after weighing to be steamed
It in distilled water, stirs evenly and is placed under 90~100 DEG C of water bath with thermostatic control after 1~2h, be then transferred into high shear dispersion machine and divide
Dissipate processing, be then transferred into enzymatic vessel, according to volume ratio be added 8~12% chitosan enzyme, heated constant temperature stirring under into
Row enzyme digestion reaction increases the temperature to 100 DEG C after reaction, and Continuous Heat inactivates 15~30min, is then rapidly cooled to room temperature,
Centrifugation, obtained sediment are washed with distilled water 3~5 times, and chitosan is made in sediment drying；
Step 3 after cleaning the fresh witloof in step 1, is placed in 2~5min in boiling water, and taking-up is cooled to room temperature, after slice,
Be dipped into 10~60s in the ethanol solution dissolved with vitamin E, it is dry after taking-up, crush, the coarse powder raw material obtained after sieving according to
Expect night than 1：10~1：20 add water mixing, are 4.0~5.0 with dilute hydrochloric acid adjustment pH value, are then transferred into high shear dispersion machine
Decentralized processing is then transferred into enzymatic vessel, and the neutral protease enzymolysis of 25mg/l~28mg/l is added, is filtered to remove filter later
Slag obtains the first leaching liquor of inulin；
First leaching liquor of inulin made from step 3 is filtered, filtered filtrate by step 4 by Ultra filtration membrane equipment
It enters back into nanofiltration membrane separation plant to be filtered, the inulin concentrate obtained after filtered trapped fluid is evaporated under reduced pressure, for use；
Step 5, the sodium acetate solution that configuration concentration is 0.15~0.3mol/l, it is 3~4.5 then to adjust pH value with acetic acid, will
Chitosan prepared by step 2 is according to mass ratio 0.5~1：10 are added to sodium acetate solution, and it is 1.0% that mass concentration, which is then added,
Aspergillus niger, make enzyme bottom ratio be 2~4%, stir 10~30min after, be transferred completely into decentralized processing in high shear dispersion machine, instead
100 DEG C are increased the temperature to after answering, Continuous Heat inactivates 15~30min, is then rapidly cooled to room temperature, chitosan oligosaccharide is obtained by filtration
First filtrate；
First filtrate of chitosan oligosaccharide made from step 5 is filtered, filtered filtrate by step 6 by Ultra filtration membrane equipment
Nanofiltration membrane separation plant is entered back into be filtered, filtered trapped fluid be soluble solid content be mass fraction 20~
25%, the chitosan oligosaccharide concentrate obtained after filtered trapped fluid is evaporated under reduced pressure, for use；
Step 7, by the inulin concentrate that step 5 obtains and the chitosan oligosaccharide concentrate that step 6 obtains after mixing, freezing is dry
Dry, vacuum seal bag packaging is made with the complex polysaccharide electuary for adjusting intestinal flora function.
2. the preparation method with the complex polysaccharide electuary for adjusting intestinal flora function according to claim 1, feature
It is：20~30min of rotating speed cutting decentralized processing that the processing of high shear dispersion machine is 2500~3000r/min in step 2；Enzymolysis
The enzyme activity degree of chitosan enzyme is 200~300U/ml, 45~50 DEG C of reaction temperature, 200~500rad/ of speed of agitator in reaction
Min, 3~4h of reaction time, enzyme digestion reaction reconcile pH7.4~7.6 with sodium carbonate liquor in the process；Machine centrifuge speed 12000
~20000rad/min centrifuges 10~20min.
3. the preparation method with the complex polysaccharide electuary for adjusting intestinal flora function according to claim 1, feature
It is：The content of vitamin E is 10~30g/l in ethanol solution in step 3；The processing of high shear dispersion machine is 2500~3000r/
20~30min of rotating speed cutting decentralized processing of min；Enzymatic hydrolysis condition is 70~75 DEG C of 1~2h of processing.
4. the preparation method with the complex polysaccharide electuary for adjusting intestinal flora function according to claim 1, feature
It is：It is mass fraction 20~25% that filtered trapped fluid, which is soluble solid content, in step 4；The pressure of vacuum distillation
Temperature for -100~-85kPa, vacuum distillation is 40~45 DEG C, the time is 20~60min；The inulin concentrate it is dense
It is mass fraction 35~40% that degree, which is soluble solid content,.
5. the preparation method with the complex polysaccharide electuary for adjusting intestinal flora function according to claim 1, feature
It is：It is 45~50 DEG C that the processing of high shear dispersion machine, which is temperature, in step 5, is disperseed with the rotating speed cutting of 2500~3000r/min
Handle 3~6h.
6. the preparation method with the complex polysaccharide electuary for adjusting intestinal flora function according to claim 1, feature
It is：The pressure being evaporated under reduced pressure in step 6 is -100~-85kPa, the temperature of vacuum distillation is 30~35 DEG C, the time be 20~
60min；A concentration of soluble solid content of the chitosan oligosaccharide concentrate is mass fraction 35~40%.
7. the preparation method with the complex polysaccharide electuary for adjusting intestinal flora function according to claim 1, feature
It is：In step 7 be freeze-dried condition be at -20 DEG C pre-freeze 2h, be placed in vacuum freeze drier, be evacuated to 10
~100Pa, -35~-40 DEG C of cold hydrazine temperature, 4~8h of cooling time.
8. a kind of having the complex polysaccharide electuary for adjusting intestinal flora function, it is characterised in that：Described having adjusts enterobacteriaceae
The complex polysaccharide electuary of group's function is made of 50~1000 parts of chitin, 50~1000 parts of fresh witloof；Described has
The complex polysaccharide electuary for adjusting intestinal flora function is made of following methods：
Step 1, the chitin for weighing 50~1000 parts respectively according to parts by weight, 50~1000 parts of fresh witloof；
Step 2 is 1000g according to solid-liquid ratio after sieving with 100 mesh sieve the chitin in step 1：10~20L is added after weighing to be steamed
It in distilled water, stirs evenly and is placed under 90~100 DEG C of water bath with thermostatic control after 1~2h, be then transferred into high shear dispersion machine and divide
Dissipate processing, be then transferred into enzymatic vessel, according to volume ratio be added 8~12% chitosan enzyme, heated constant temperature stirring under into
Row enzyme digestion reaction increases the temperature to 100 DEG C after reaction, and Continuous Heat inactivates 15~30min, is then rapidly cooled to room temperature,
Centrifugation, obtained sediment are washed with distilled water 3~5 times, and chitosan is made in sediment drying；
Step 3 after cleaning the fresh witloof in step 1, is placed in 2~5min in boiling water, and taking-up is cooled to room temperature, after slice,
Be dipped into 10~60s in the ethanol solution dissolved with vitamin E, it is dry after taking-up, crush, the coarse powder raw material obtained after sieving according to
Expect night than 1：10~1：20 add water mixing, are 4.0~5.0 with dilute hydrochloric acid adjustment pH value, are then transferred into high shear dispersion machine
Decentralized processing is then transferred into enzymatic vessel, and the neutral protease enzymolysis of 25mg/l~28mg/l is added, is filtered to remove filter later
Slag obtains the first leaching liquor of inulin；
First leaching liquor of inulin made from step 3 is filtered, filtered filtrate by step 4 by Ultra filtration membrane equipment
It enters back into nanofiltration membrane separation plant to be filtered, the inulin concentrate obtained after filtered trapped fluid is evaporated under reduced pressure, for use；
Step 5, the sodium acetate solution that configuration concentration is 0.15~0.3mol/l, it is 3~4.5 then to adjust pH value with acetic acid, will
Chitosan prepared by step 2 is according to mass ratio 0.5~1：10 are added to sodium acetate solution, and it is 1.0% that mass concentration, which is then added,
Aspergillus niger, make enzyme bottom ratio be 2~4%, stir 10~30min after, be transferred completely into decentralized processing in high shear dispersion machine, instead
100 DEG C are increased the temperature to after answering, Continuous Heat inactivates 15~30min, is then rapidly cooled to room temperature, chitosan oligosaccharide is obtained by filtration
First filtrate；
First filtrate of chitosan oligosaccharide made from step 5 is filtered, filtered filtrate by step 6 by Ultra filtration membrane equipment
Nanofiltration membrane separation plant is entered back into be filtered, filtered trapped fluid be soluble solid content be mass fraction 20~
25%, the chitosan oligosaccharide concentrate obtained after filtered trapped fluid is evaporated under reduced pressure, for use；
Step 7, by the inulin concentrate that step 5 obtains and the chitosan oligosaccharide concentrate that step 6 obtains after mixing, freezing is dry
Dry, vacuum seal bag packaging is made with the complex polysaccharide electuary for adjusting intestinal flora function.
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