CN108552451B - Functional beverage after sports - Google Patents

Functional beverage after sports Download PDF

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CN108552451B
CN108552451B CN201810326524.8A CN201810326524A CN108552451B CN 108552451 B CN108552451 B CN 108552451B CN 201810326524 A CN201810326524 A CN 201810326524A CN 108552451 B CN108552451 B CN 108552451B
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vitamin
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CN108552451A (en
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朱德文
黄广原
张海峰
马啸
杜玉涛
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Shenzhen Bgi Sports Holding Co ltd
Bgi Precision Nutrition Shenzhen Technology Co ltd
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Shenzhen Bgi Sports Holding Co ltd
Bgi Precision Nutrition Shenzhen Technology Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38—Other non-alcoholic beverages
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/175—Amino acids
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Health & Medical Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
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Abstract

The invention discloses a functional beverage after sports. The composition of amino acids provided in the present invention comprises 7 effective ingredients: hydroxyproline, proline, glutamic acid, theanine, L-leucine, L-isoleucine and L-valine; the mass ratio is as follows in sequence: 0.2-1: 0.2-1: 2-5: 0.05-0.3: 1-3: 0.5-1.5: 0.5-1.5. The product of the invention comprises the following raw materials: wheat protein, whole milk powder, hydrolyzed whey protein peptide, whey protein powder, soybean protein isolate powder, collagen, branched chain amino acid, tea amino acid, crystalline fructose, fructo-oligosaccharide, sodium carboxymethylcellulose, resistant dextrin, taurine, vitamin premix and mineral premix. The composition or the product can be used for: preparing a functional beverage to be taken after exercise; can be used as functional beverage for patients after exercise. The invention fills the blank of accurate nutrition supply of the ultimate endurance events in China.

Description

Functional beverage after sports
Technical Field
The invention relates to a functional beverage after sports.
Background
At present, various protein powder, branched chain amino acid BCAAs, L-glutamine, theanine and collagen supplement nutriments are available on the market, but most of the nutriments are limited to one or the combination of one or two of the protein powder, and the synergic research of various functional amino acids based on Chinese population data is almost blank.
The prior art has slow effect because of single functional component. Especially, basic data research of large sample multiomics and comprehensive research data of Chinese people are lacked, so that recovery products are often not considered comprehensively after corresponding endurance sports are designed, and no sports recovery products particularly aiming at specific national constitutions or accurate nutrition supplement products aiming at high-strength endurance sports events are available.
Hydroxyproline and proline are nutrients which are easy to lose after strenuous exercise, and the existing sports nutrition products in the market are less added, and are particularly applied to extreme endurance events.
Disclosure of Invention
The invention aims to provide a functional beverage after sports.
The invention firstly provides a composition for providing amino acid, which comprises the following 7 effective components: hydroxyproline, proline, glutamic acid, theanine, L-leucine, L-isoleucine and L-valine;
the mass ratio of hydroxyproline, proline, glutamic acid, theanine, L-leucine, L-isoleucine and L-valine is as follows in sequence: 0.2-1: 0.2-1: 2-5: 0.05-0.3: 1-3: 0.5-1.5: 0.5-1.5.
The mass ratio of hydroxyproline, proline, glutamic acid, theanine, L-leucine, L-isoleucine and L-valine is as follows in sequence: 0.7: 0.9 g: 3.6 g: 0.07: 2.13: 1.12: 1.12.
the raw materials of the composition comprise: wheat protein (specifically concentrated wheat protein), whole milk powder, hydrolyzed whey protein peptide, whey protein powder, soybean protein isolate powder, collagen protein, branched chain amino acid (specifically branched chain amino acid microcapsule powder), and tea amino acid.
The composition comprises the following raw materials: wheat protein (specifically concentrated wheat protein), whole milk powder, hydrolyzed whey protein peptide, whey protein powder, soybean protein isolate powder, collagen protein, branched chain amino acid (specifically branched chain amino acid microcapsule powder), and tea amino acid.
The weight ratio of wheat protein (specifically concentrated wheat protein), whole milk powder, hydrolyzed whey protein peptide, whey protein powder, soybean protein isolate powder, collagen, branched chain amino acid (specifically branched chain amino acid microcapsule powder) and tea amino acid is as follows: 6.37: 4.5: 2.15: 1.5: 1.5: 1.43: 2.8: 0.28.
the invention also provides a product comprising the composition as described in any one of the above.
The raw materials of the product comprise: wheat protein (specifically concentrated wheat protein), whole milk powder, hydrolyzed whey protein peptide, whey protein powder, soybean protein isolate powder, collagen protein, branched chain amino acid ()
The raw materials of the product comprise: wheat protein (specifically concentrated wheat protein), whole milk powder, hydrolyzed whey protein peptide, whey protein powder, soybean protein isolate powder, collagen, branched chain amino acid (specifically branched chain amino acid microcapsule powder), tea amino acid, vitamin premix and mineral premix.
The mass ratio of wheat protein (specifically concentrated wheat protein), whole milk powder, hydrolyzed whey protein peptide, whey protein powder, soybean protein isolate powder, collagen, branched chain amino acid (specifically branched chain amino acid microcapsule powder), tea amino acid, vitamin premix and mineral premix is 6.37: 4.5: 2.15: 1.5: 1.5: 1.43: 2.8: 0.28: 0.13: 0.81.
the raw materials of the product comprise: wheat protein (specifically concentrated wheat protein), whole milk powder, hydrolyzed whey protein peptide, whey protein powder, soybean protein isolate powder, collagen, branched chain amino acid (specifically branched chain amino acid microcapsule powder), tea amino acid, crystalline fructose, fructo-oligosaccharide, sodium carboxymethylcellulose, resistant dextrin, taurine, vitamin premix and mineral premix.
The raw materials of the product comprise: wheat protein (specifically concentrated wheat protein), whole milk powder, hydrolyzed whey protein peptide, whey protein powder, soybean protein isolate powder, collagen, branched chain amino acid (specifically branched chain amino acid microcapsule powder), tea amino acid, crystalline fructose, fructo-oligosaccharide, sodium carboxymethylcellulose, resistant dextrin, taurine, vitamin premix and mineral premix.
The mass ratio of wheat protein (specifically concentrated wheat protein), whole milk powder, hydrolyzed whey protein peptide, whey protein powder, soybean protein isolate powder, collagen, branched chain amino acid (specifically branched chain amino acid microcapsule powder), tea amino acid, crystalline fructose, fructo-oligosaccharide, sodium carboxymethyl cellulose, resistant dextrin, taurine, vitamin premix and mineral premix is 6.37: 4.5: 2.15: 1.5: 1.5: 1.43: 2.8: 0.28: 5.07: 0.9: 0.8: 1.72: 0.04: 0.13: 0.81.
the invention also provides a preparation method of the product, which comprises the following steps:
(1) mixing vitamin premix, mineral premix, tea amino acid and taurine to obtain a mixed material A;
(2) mixing the mixture A, soybean protein isolate powder, branched chain amino acid (specifically branched chain amino acid microcapsule powder), resistant dextrin, sodium carboxymethylcellulose, fructo-oligosaccharide, collagen, whey protein powder, hydrolyzed whey protein peptide, whole milk powder, crystalline fructose, and wheat protein (specifically concentrated wheat protein), and mixing to obtain the final product.
In the preparation method of the product, the mass ratio of the wheat protein (specifically concentrated wheat protein), the whole milk powder, the hydrolyzed whey protein peptide, the whey protein powder, the isolated soy protein powder, the collagen, the branched chain amino acid (specifically branched chain amino acid microcapsule powder), the tea amino acid, the crystalline fructose, the fructo-oligosaccharide, the sodium carboxymethylcellulose, the resistant dextrin, the taurine, the vitamin premix and the mineral premix is 6.37: 4.5: 2.15: 1.5: 1.5: 1.43: 2.8: 0.28: 5.07: 0.9: 0.8: 1.72: 0.04: 0.13: 0.81.
the product prepared by the method also belongs to the protection scope of the invention.
The function of any of the above products is (a1), (a2) or (a 3):
(a1) providing an amino acid;
(a2) preparing a functional beverage to be taken after exercise;
(a3) can be used as functional beverage for patients after exercise.
The composition or the product provided by the invention can be used in the fields of food and health care products.
The compositions or products provided by the present invention may be used in a variety of dosage forms. Such as solid beverages, liquid beverages, energy gels, energy bars (nutritional bars), capsules, tablets, and the like.
The invention also protects the application of any one of the compositions or the products, which is (b1) or (b 2):
(b1) preparing a functional beverage to be taken after exercise;
(b2) can be used as functional beverage for patients after exercise.
The functional beverage is solid beverage or liquid beverage. When the functional beverage is a solid beverage, the functional beverage can be drunk after being mixed with water.
Any one of the vitamin premixes comprises the following 12 effective components: the component I-1, the component I-2, the component I-3, the component I-4, the component I-5, the component I-6, the component I-7, the component I-8, the component I-9, the component I-10, the component I-11 and the component I-12;
component I-1 provides a substance having a vitamin A function, component I-2 provides a substance having a vitamin B1 function, component I-3 provides a substance having a vitamin B2 function, component I-4 provides a substance having a vitamin B3 function, component I-5 provides a substance having a vitamin B5 function, component I-6 provides a substance having a vitamin B6 function, component I-7 provides a substance having a vitamin B9 function, component I-8 provides a substance having a vitamin B12 function, component I-9 provides a substance having a vitamin C function, component I-10 provides a substance having a vitamin D function, component I-11 provides a substance having a vitamin E function, and component I-12 provides a substance having a vitamin H function;
the proportion of the component I-1, the component I-2, the component I-3, the component I-4, the component I-5, the component I-6, the component I-7, the component I-8, the component I-9, the component I-10, the component I-11 and the component I-12 is 1100-: 14.22-21.33 mg: 7.112-10.67 mg: 49.11-73.67 mg: 17.33-26.00 mg: 7.112-10.67 mg: 888.9-1333 μ g: 14.22-21.33. mu.g: 255.5-383.3 mg: 25.55-38.33. mu.g: 49.12-73.68 mg: 121.12-181.68 μ g.
Any one of the mineral premix comprises the following 9 effective components: component II-1, component II-2, component II-3, component II-4, component II-5, component II-6, component II-7, component II-8 and component II-9;
the component II-1 provides a sodium element, the component II-2 provides a phosphorus element, the component II-3 provides a potassium element, the component II-4 provides a magnesium element, the component II-5 provides a calcium element, the component II-6 provides an iron element, the component II-7 provides a zinc element, the component II-8 provides a selenium element, and the component II-9 provides an iodine element;
the ratio of sodium element provided by the component II-1, phosphorus element provided by the component II-2, potassium element provided by the component II-3, magnesium element provided by the component II-4, calcium element provided by the component II-5, iron element provided by the component II-6, zinc element provided by the component II-7, selenium element provided by the component II-8 and iodine element provided by the component II-9 is 85.19-127.8mg in sequence: 40.92-61.39 mg: 85.18-127.8 mg: 13.08-19.61 mg: 35.19-52.78 mg: 1.2088-1.8134 mg: 1.4226-2.134 mg: 2.208-3.312 μ g: 3.614-5.420 μ g.
The mixture ratio of the component I-1, the component I-2, the component I-3, the component I-4, the component I-5, the component I-6, the component I-7, the component I-8, the component I-9, the component I-10, the component I-11 and the component I-12 is 1375 mu g: 17.78 mg: 8.89 mg: 61.39 mg: 21.67 mg: 8.89 mg: 1111 μ g: 17.78 μ g: 319.4 mg: 31.94 μ g: 61.4 mg: 151.4 μ g.
The ratio of sodium element provided by the component II-1, phosphorus element provided by the component II-2, potassium element provided by the component II-3, magnesium element provided by the component II-4, calcium element provided by the component II-5, iron element provided by the component II-6, zinc element provided by the component II-7, selenium element provided by the component II-8 and iodine element provided by the component II-9 is 106.5 mg: 51.16 mg: 106.49 mg: 16.35 mg: 43.99 mg: 1.52 mg: 1.78 mg: 2.76 μ g: 4.52. mu.g.
The vitamin premix consists of a component I-1, a component I-2, a component I-3, a component I-4, a component I-5, a component I-6, a component I-7, a component I-8, a component I-9, a component I-10, a component I-11, a component I-12 and a filler.
Each 1g of the vitamin premix comprises a component I-1, a component I-2, a component I-3, a component I-4, a component I-5, a component I-6, a component I-7, a component I-8, a component I-9, a component I-10, a component I-11 and a component I-12 which are 1100-1650 mu g, 14.22-21.33mg, 7.112-10.67mg, 49.11-73.67mg, 17.33-26.00mg, 7.112-10.67mg, 888.9-1333 mu g, 14.22-21.33 mu g, 255.5-383.3mg, 25.55-38.33 mu g, 49.12-73.68mg and 121.12-181.68 mu g in sequence.
The mineral premix consists of a component II-1, a component II-2, a component II-3, a component II-4, a component II-5, a component II-6, a component II-7, a component II-8, a component II-9 and a filler.
Each 1g of the mineral premix contains sodium provided by the component II-1, phosphorus provided by the component II-2, potassium provided by the component II-3, magnesium provided by the component II-4, calcium provided by the component II-5, iron provided by the component II-6, zinc provided by the component II-7, selenium provided by the component II-8 and iodine provided by the component II-9 in the order of 85.19-127.8mg, 40.92-61.39mg, 85.18-127.8mg, 13.08-19.61mg, 35.19-52.78mg, 1.2088-1.8134mg, 1.4226-2.134mg, 2.208-3.312 μ g and 3.614-5.420 μ g.
Each 1g of the vitamin premix comprises 1375 mug of component I-1, 17.78mg, 8.89mg, 61.39mg, 21.67mg, 8.89mg, 1111 mug of, 17.78 mug of, 319.4mg, 31.94 mug of, 61.4mg and 151.4 mug of component I-3, component I-4, component I-5, component I-6, component I-7, component I-8, component I-9, component I-10, component I-11 and component I-12 in sequence.
The contents of sodium element provided by the component II-1, phosphorus element provided by the component II-2, potassium element provided by the component II-3, magnesium element provided by the component II-4, calcium element provided by the component II-5, iron element provided by the component II-6, zinc element provided by the component II-7, selenium element provided by the component II-8 and iodine element provided by the component II-9 in each 1g of the mineral premix were 106.5mg, 51.16mg, 106.49mg, 16.35mg, 43.99mg, 1.52mg, 1.78mg, 2.76 μ g and 4.52 μ g, respectively.
The vitamin A acetate is used as the component I-1, the thiamine hydrochloride is used as the component I-2, the riboflavin is used as the component I-3, the nicotinamide is used as the component I-4, the calcium D-pantothenate is used as the component I-5, the pyridoxine hydrochloride is used as the component I-6, the vitamin B9 is used as the component I-7, the cyanocobalamine is used as the component I-8, the sodium L-ascorbate is used as the component I-9, the vitamin D3 is used as the component I-10, the dl-alpha-tocopherol acetate is used as the component I-11, and the D-biotin is used as the component I-12.
The component II-1 is sodium citrate and/or disodium hydrogen phosphate, the component II-2 is disodium hydrogen phosphate, the component II-3 is potassium citrate and/or potassium chloride, the component II-4 is magnesium carbonate, the component II-5 is calcium carbonate, the component II-6 is ferric pyrophosphate, the component II-7 is zinc sulfate, the component II-8 is sodium selenite, and the component II-9 is potassium iodate.
The composition provided by the invention is based on comprehensive human multigroup data before and after endurance exercise, functional nutrients and easily-lost nutrients are determined by monitoring the change condition of the nutrients, and accurate supplement of amino acid components such as branched-chain amino acid BCAAs (valine, leucine, isoleucine), glutamine, theanine, hydroxyproline, proline and the like is realized by combining several food raw materials such as concentrated wheat protein, whole milk powder, hydrolyzed whey protein peptide, whey protein powder, soybean protein isolate powder, collagen, branched-chain amino acid microcapsule powder and tea amino acid, so that the body recovery after endurance exercise is rapidly promoted.
The functional beverage can achieve the following effects: inhibiting creatine kinase fluctuation, increasing levels of valine, L-leucine, L-isoleucine and proline, and supplementing functional beverage after exercise for a certain time to relieve exercise injury and accelerate repair of injury after exercise. The composition provided by the invention is mainly characterized in that nutrients are integrally and comprehensively supplemented based on multiple groups of mathematical data, so that the effect is better than that of a single or individual combination of nutrients.
The invention fills the blank of accurate nutrition supply of the ultimate endurance events in China.
Drawings
FIG. 1 shows the average fluctuation ratio of amino acids.
FIG. 2 is the mean hormone fluctuation rate.
Fig. 3 is a graph of creatine kinase changes at different time points.
Figure 4 is a graph of the change in creatine kinase levels at different time points in a subject.
FIG. 5 is a graph of valine level changes at different time points in subjects.
FIG. 6 is a graph of the change in L-isoleucine levels at different time points in a subject.
FIG. 7 is a graph of changes in L-leucine levels at different time points in a subject.
Figure 8 is a graph of changes in proline levels at different time points in subjects.
Detailed Description
The following examples are given to facilitate a better understanding of the invention, but do not limit the invention. The experimental procedures in the following examples are conventional unless otherwise specified. The test materials used in the following examples were purchased from a conventional biochemical reagent store unless otherwise specified. The quantitative tests in the following examples, all set up three replicates and the results averaged. The content of each amino acid is detected by adopting a method for measuring the amino acid in the food in GB/T5009.124-2003. Theanine (L-Theanine) is a free amino acid unique to tea leaves and is glutamic acid γ -ethylamide. Detecting the content of theanine in the amino acid of the tea by adopting a method for measuring the total amount of free amino acid in the tea in GB/T8314-2013. Maltodextrin, 2: west king sugar industry Co.
The concentrated wheat protein in the examples is SYRAL wheat protein, Shanghai jicheng,
Figure BDA0001626755830000061
500，http://www.jebsenindustrial.com/％E5％85％B3％E4％BA％8E％E6％88％91％E4％BB％AC/％E5％90％88％E4％BD％9C％E4％BC％99％E4％BC％B4/syral/syral％E5％B0％8F％E9％BA％A6％E8％9B％8B％E7％99％BD/。
the whole milk powder used in the examples was a natural whole milk powder, cat # 113120.
The hydrolyzed whey protein peptide used in the examples was
Figure BDA0001626755830000062
Peili peptide: disemann, cat # 5007143.
The whey protein powder used in the examples was HILMAR8010 concentrated whey protein powder.
The soy protein isolate used in the examples was DuPont SUPRO 787 soy protein isolate.
The collagen used in the examples was fish collagen peptide: shanghai Tongyuan health care science and technology, goods number GBB-50SP 757.
Branched chain amino acid microcapsule powders used in examples: dalian Deno Bio Inc., cat # 60102-P.
The tea amino acids used in the examples were natural tea amino acids (the Shanghai Node organism is a natural tea amino acid from Engineer, Inc., Tennova TM L20) and the website is http:// www.novanat.com/showproduct. aspag ═ c & id ═ 80.
Example 1 formulation development of functional drink after exercise
First, formula development process
The eight hundred quicksand limit event is the first super-long-distance uninterrupted, self-navigation and self-loading cross-country running event in China; and (4) limit examination: high-load motion state, ultra-long distance and severe environment; limited supply and lack of rest, and the physiological function of athletes can be tested. The Huada sports is used as a Huada gene flag setting mechanism, and provides the athlete with omnibearing detection services before, during, after and two weeks after the match. The assessment is carried out from the aspects of gene, metabolism, sports physiology, psychology, overall sports health of athletes and the like, and personalized suggestions are provided, so that a basis is provided for making a more scientific sports plan.
Amino acid fluctuation rate (post-race level-pre-race level)/pre-race level × 100%. The average fluctuation rate of amino acids is shown in FIG. 1, with negative values indicating decreased levels and positive values indicating increased levels. In fig. 1, hydroxyproline, proline, ornithine, valine, alanine, sarcosine, cysteine, isoleucine, leucine, phosphoserine, citrulline, glycine, threonine, γ -hydroxylysine, lysine, serine, tryptophan, α -amino-n-butyric acid, and asparagine are sequentially arranged from left to right. The average fluctuation rate of hydroxyproline is-54%, the average fluctuation rate of proline is-47%, the average fluctuation rate of ornithine is-44%, the average fluctuation rate of valine is-39%, the average fluctuation rate of alanine is-38%, the average fluctuation rate of sarcosine is-32%, the average fluctuation rate of cysteine is-30%, the average fluctuation rate of isoleucine is-24%, the average fluctuation rate of leucine is-24%, the average fluctuation rate of phosphoserine is-22%, the average fluctuation rate of citrulline is-22%, the average fluctuation rate of glycine is-22%, the average fluctuation rate of threonine is-20%, the average fluctuation rate of gamma-hydroxylysine is-17%, the average fluctuation rate of lysine is-11%, the average fluctuation rate of serine is-9.3%, The mean fluctuation ratio of tryptophan was-7.5%, the mean fluctuation ratio of α -aminon-butyric acid was-6.4%, and the mean fluctuation ratio of asparagine was-4.7%. Athletes lose more of 3 Branched Chain Amino Acids (BCAAs) (valine, isoleucine, leucine): the loss ratio of the three amino acids is 24-40%. The loss of the branched chain amino acid BCAAs accelerates the muscle decomposition process, generates stronger sports fatigue feeling and reduces the sports ability. The two amino acids with the greatest player churn rate are hydroxyproline and proline. Collagen contains hydroxyproline and proline in high proportion, and the specific amino acids are nutrients which are easy to lose after strenuous exercise. Collagen plays an important role in the development, differentiation and formation of individuals as well as other connective tissues, and is closely related to the aging and diseases of the body.
Hormone fluctuation rate ═ (post-race level-pre-race level)/pre-race level × 100%. The mean hormone fluctuation is shown in FIG. 2, with negative values being lower and positive values being higher. In fig. 2, testosterone/cortisol, testosterone, 17-hydroxyprogesterone, progesterone, androstenedione, dehydroepiandrosterone, 11-deoxycorticosterone, cortisone, 11-deoxycorticosterol, estradiol, aldosterone, dihydrotestosterone, dehydroepiandrosterone sulfate, cortisol, estrone, and corticosterone are arranged in this order from left to right. The mean fluctuation rate of testosterone/cortisol was-75%, the mean fluctuation rate of testosterone was-63%, the mean fluctuation rate of 17-hydroxyprogesterone was-47%, the mean fluctuation rate of progesterone was-40%, the mean fluctuation rate of androstenedione was-38%, the mean fluctuation rate of dehydroepiandrosterone was-12%, the mean fluctuation rate of 11-deoxycorticosterone was 3.33%, the mean fluctuation rate of cortisone was 3.86%, the mean fluctuation rate of 11-deoxycorticosterol was 16.4%, the mean fluctuation rate of estradiol was 58.7%, the mean fluctuation rate of aldosterone was 69.6%, the mean fluctuation rate of dihydrotestosterone was 91.6%, the mean fluctuation rate of dehydroepiandrosterone sulfate was 107%, the mean fluctuation rate of cortisol was 126%, the mean fluctuation rate of estrone was 165%, and the mean fluctuation rate of corticosterone was 230%. Testosterone: muscle loss is accelerated. Cortisol: increase in body fatigue, and accelerate the decomposition of saccharides, fats and proteins. Testosterone/cortisol ratio: the reduction amplitude is more than 30%, and the athlete is excessive in movement on the whole.
Creatine kinase, lactate dehydrogenase, aspartate aminotransferase and alanine aminotransferase four muscle metabolic enzymes can be used to assess the degree of muscle damage. The graph of creatine kinase changes at different time points is shown in fig. 3. In fig. 3, time1 is before the start of the race, time 2 is 2 days after the start of the race (sixth replenishing point), time3 is after the end of the race, and time4 is 2 weeks after the end of the race. The four muscle enzymes have similar variation trends: normal before the race; the RP6 point (the sixth supply point) in the game rises to the highest value; decline after the race, do not return to the normal level; the decline continued two weeks after the race, but still did not return to the pre-race normal level. The eight hundred quicksand limit race has certain damage to the muscles of athletes, and the muscles of the athletes still do not recover to the normal level before the race even two weeks after the race.
Through continuous monitoring of athletes at different stages before, during and after the competition, the athlete finds that the loss rate of the amino acid level is high, especially branched chain amino acid, proline and hydroxyproline. At the same time, the hormone level (testosterone/cortisol ratio) is reduced by more than 30%, reflecting that long-term endurance exercise has obvious negative effects on the athlete's body, and the recovery is slow. Therefore, the corresponding nutritional support before, during and after the match is a very important link, and the body comfort of athletes can be improved by pertinently supplementing corresponding amino acids and adjusting related hormone levels, so that the repair of various functions of the body is accelerated.
Second, the core design of the formula
The core design of the formulation is shown in table 1.
TABLE 1
Component name Mass ratio of Design amount per day
Hydroxyproline 0.2-1 0.2-1g
Proline 0.2-1 0.2-1g
Glutamic acid 2-5 2-5g
Theanine 0.05-0.3 0.05-0.3g
L-leucine 1-3 1-3g
L-isoleucine 0.5-1.5 0.5-1.5g
L-valine 0.5-1.5 0.5-1.5g
Example 2 preparation of premix
Vitamin premix
The vitamin premix consists of functional raw materials and a filler. The filler is maltodextrin. The content of each functional material in each 1g of the vitamin premix is shown in table 2.
TABLE 2
Figure BDA0001626755830000081
Figure BDA0001626755830000091
The vitamin premix used in example 3 was composed of functional raw materials and a filler. The filler is maltodextrin. The content of each functional material in the vitamin premix per unit mass is shown in table 3.
TABLE 3
Figure BDA0001626755830000092
Second, mineral premix
The mineral premix consists of functional raw materials and a filler. The filler is maltodextrin. The composition of each functional raw material per 1g of the mineral premix and the amount of the corresponding functional element provided by each functional raw material are shown in table 4.
TABLE 4
Figure BDA0001626755830000093
The mineral premix used in example 3 was composed of functional raw materials and fillers. The filler is maltodextrin. The contents of the respective functional materials in the mineral premix per unit mass are shown in table 5.
TABLE 5
Figure BDA0001626755830000094
Figure BDA0001626755830000101
Example 3 preparation of functional drink after exercise (solid drink)
1 person in 1 day after 1 bag of sports functional beverage, and 30g after 1 bag of sports functional beverage. The composition of the raw materials of the functional drink after exercise, the dosage (g) of the raw materials in each 30g of the functional drink after exercise, and the amount (g) of the nutrient elements provided by the raw materials in each 30g of the functional drink after exercise are shown in Table 6.
TABLE 6
Figure BDA0001626755830000102
Crystalline fructose: bowling Bao Bio Inc., cat # QR 0256. Fructo-oligosaccharide: SENSUS, cat No. 513E 801602. Sodium carboxymethylcellulose: the product number is FH 6. Resistant dextrin: batoka (shanghai) commercial ltd, cat # 338111W. Taurine: hubei Dashenghuashengshi science and technology, Inc., Cat No. C002-M1710621.
Crystalline fructose serves as an energy supplement and sweetener.
The fructo-oligosaccharide can be used as sweetener and intestinal buffer.
Sodium carboxymethyl cellulose is used as an adhesive.
The resistant dextrin acts as a bulking agent.
Taurine is used as a basic nutrient supplement.
The preparation process comprises the following steps:
1. fully mixing the vitamin premix, the mineral premix, the tea amino acid and the taurine to obtain a mixed material A.
2. The preparation method comprises the following steps of fully mixing a mixed material A, soybean protein isolate powder, branched chain amino acid microcapsule powder, resistant dextrin, sodium carboxymethylcellulose, fructo-oligosaccharide, collagen, whey protein powder, hydrolyzed whey protein peptide, whole milk powder, crystalline fructose and concentrated wheat protein to obtain a mixed material B.
3. And subpackaging the mixed material B, and 30g of the mixed material B is packaged in each bag.
According to detection, every 30g of functional beverage after exercise contains 3.6g of glutamic acid, 0.9g of proline, 0.7g of hydroxyproline, 2.13g of L-leucine, 1.12g of L-isoleucine, 1.12g of L-valine and 0.07g of theanine.
Example 4 Effect verification of functional beverages after exercise
The 60 runners (all volunteers with informed consent) were randomly divided into two groups of 30 each, using a randomized, parallel-control clinical trial.
Experimental groups (each as follows):
venous blood was collected 8 o' clock (time1) late on day 0 of the experiment;
day 1 to day 4 of the experiment: running for 20 km long in the morning (the running speed is controlled at 10 km/h), and taking a bag of functional beverage after exercise (taken after being mixed with boiled water) prepared in example 31 hour after running;
venous blood was collected on day 1 at 8 o 'clock (time1), day 4 at 8 o' clock (time3), day 7 at 8 o 'clock (time4), and day 14 at 8 o' clock (time5), respectively.
The control group did not take the functional drink after exercise prepared in example 3, and the other groups were the same as the experimental group.
The main functions of branched-chain amino acids (valine, leucine, isoleucine): improving exercise ability and delaying fatigue. Branched chain amino acids are a general term for 3 of 8 kinds of amino acids essential to the body, are essential nutrients for muscles, and account for 30% -40% of amino acids constituting muscles. Function of branched chain amino acids: the energy storage device is closely related to the exercise capacity of the human body and can provide energy storage for long-time endurance exercise; promoting muscle protein synthesis; anti-decomposition effects, which help to prevent protein decomposition and muscle loss, and to protect muscles; regulating a peripheral mechanism for inducing the exercise-induced fatigue and postponing the appearance of the exercise-induced fatigue; supplementation with branched-chain amino acids after exercise may also promote recovery from exercise-induced fatigue.
The levels of Creatine Kinase (CK), valine, L-leucine, L-isoleucine and proline in the plasma of venous blood collected at 5 time points were measured to evaluate the extent of exercise injury.
The graph of the creatine kinase level at different time points of the subject is shown in FIG. 4 (ordinate units are U/L). The profile of the valine levels at different time points is shown in FIG. 5 (ordinate units μmol/L). The L-isoleucine levels at different time points for the subjects are plotted in FIG. 6 (ordinate units μmol/L). The L-leucine level profile for the subjects at different time points is shown in FIG. 7 (ordinate units μmol/L). The profile of the change in proline levels at different time points in the subject is shown in FIG. 8 (ordinate units μmol/L). Compared with the experimental group, the creatine kinase level of the control group has larger fluctuation, and the levels of valine, L-leucine, L-isoleucine and proline are lower. The results show that the functional beverage prepared in example 3 can slow down sports injury and accelerate the repair of sports injury by targeted supplementation.
Examples 5,
In the experimental group, volunteers were given the composition shown in table 7 (one person in table 7 for 1 day) every day instead of the functional drink after exercise, and the same as in example 4. The effect is not significantly different from example 4.
TABLE 7
Figure BDA0001626755830000121

Claims (6)

1. A composition for providing amino acids comprising the following 7 effective ingredients: hydroxyproline, proline, glutamic acid, theanine, L-leucine, L-isoleucine and L-valine;
the mass ratio of hydroxyproline, proline, glutamic acid, theanine, L-leucine, L-isoleucine and L-valine is as follows in sequence: 0.7: 0.9: 3.6: 0.07: 2.13: 1.12: 1.12;
the raw materials of the composition comprise: wheat protein, whole milk powder, hydrolyzed whey protein peptide, whey protein powder, soybean protein isolate powder, collagen, branched chain amino acid and tea amino acid; the weight ratio of wheat protein, whole milk powder, hydrolyzed whey protein peptide, whey protein powder, soybean protein isolate, collagen, branched chain amino acid and tea amino acid is as follows: 6.37: 4.5: 2.15: 1.5: 1.5: 1.43: 2.8: 0.28.
2. a product, the starting materials comprising: wheat protein, whole milk powder, hydrolyzed whey protein peptide, whey protein powder, soybean protein isolate powder, collagen, branched chain amino acid, tea amino acid, vitamin premix and mineral premix;
the mass ratio of wheat protein, whole milk powder, hydrolyzed whey protein peptide, whey protein powder, soybean protein isolate powder, collagen, branched chain amino acid, tea amino acid, vitamin premix and mineral premix is 6.37: 4.5: 2.15: 1.5: 1.5: 1.43: 2.8: 0.28: 0.13: 0.81;
the vitamin premix comprises the following 12 effective components: the component I-1, the component I-2, the component I-3, the component I-4, the component I-5, the component I-6, the component I-7, the component I-8, the component I-9, the component I-10, the component I-11 and the component I-12; component I-1 provides a substance having a vitamin A function, component I-2 provides a substance having a vitamin B1 function, component I-3 provides a substance having a vitamin B2 function, component I-4 provides a substance having a vitamin B3 function, component I-5 provides a substance having a vitamin B5 function, component I-6 provides a substance having a vitamin B6 function, component I-7 provides a substance having a vitamin B9 function, component I-8 provides a substance having a vitamin B12 function, component I-9 provides a substance having a vitamin C function, component I-10 provides a substance having a vitamin D function, component I-11 provides a substance having a vitamin E function, and component I-12 provides a substance having a vitamin H function; each 1g of the vitamin premix comprises a component I-1, a component I-2, a component I-3, a component I-4, a component I-5, a component I-6, a component I-7, a component I-8, a component I-9, a component I-10, a component I-11 and a component I-12 which are 1100-1650 mu g, 14.22-21.33mg, 7.112-10.67mg, 49.11-73.67mg, 17.33-26.00mg, 7.112-10.67mg, 888.9-1333 mu g, 14.22-21.33 mu g, 255.5-383.3mg, 25.55-38.33 mu g, 49.12-73.68mg and 121.12-181.68 mu g in sequence;
the mineral premix comprises the following 9 effective components: component II-1, component II-2, component II-3, component II-4, component II-5, component II-6, component II-7, component II-8 and component II-9; the component II-1 provides a sodium element, the component II-2 provides a phosphorus element, the component II-3 provides a potassium element, the component II-4 provides a magnesium element, the component II-5 provides a calcium element, the component II-6 provides an iron element, the component II-7 provides a zinc element, the component II-8 provides a selenium element, and the component II-9 provides an iodine element; each 1g of the mineral premix contains sodium provided by the component II-1, phosphorus provided by the component II-2, potassium provided by the component II-3, magnesium provided by the component II-4, calcium provided by the component II-5, iron provided by the component II-6, zinc provided by the component II-7, selenium provided by the component II-8 and iodine provided by the component II-9 in sequence, wherein the contents of the sodium, the phosphorus, the potassium, the magnesium, the calcium, the iron, the zinc, the selenium and the iodine are 85.19-127.8mg, 40.92-61.39mg, 85.18-127.8mg, 13.08-19.61mg, 35.19-52.78mg, 1.2088-1.8134mg, 1.4226-2.134mg, 2.208-3.312 mug and 3.614-5.420 mug;
in the product, hydroxyproline, proline, glutamic acid, theanine, L-leucine, L-isoleucine and L-valine are sequentially mixed according to the mass ratio: 0.7: 0.9: 3.6: 0.07: 2.13: 1.12: 1.12;
the function of the product is as follows (a2) or (a 3):
(a2) preparing a functional beverage to be taken after exercise;
(a3) can be used as functional beverage for patients after exercise.
3. A product, the starting materials comprising: wheat protein, whole milk powder, hydrolyzed whey protein peptide, whey protein powder, soybean protein isolate powder, collagen, branched chain amino acid, tea amino acid, crystalline fructose, fructo-oligosaccharide, sodium carboxymethylcellulose, resistant dextrin, taurine, vitamin premix and mineral premix;
the weight ratio of wheat protein, whole milk powder, hydrolyzed whey protein peptide, whey protein powder, soybean protein isolate powder, collagen, branched chain amino acid, tea amino acid, crystalline fructose, fructo-oligosaccharide, sodium carboxymethylcellulose, resistant dextrin, taurine, vitamin premix and mineral premix is 6.37: 4.5: 2.15: 1.5: 1.5: 1.43: 2.8: 0.28: 5.07: 0.9: 0.8: 1.72: 0.04: 0.13: 0.81;
the vitamin premix comprises the following 12 effective components: the component I-1, the component I-2, the component I-3, the component I-4, the component I-5, the component I-6, the component I-7, the component I-8, the component I-9, the component I-10, the component I-11 and the component I-12; component I-1 provides a substance having a vitamin A function, component I-2 provides a substance having a vitamin B1 function, component I-3 provides a substance having a vitamin B2 function, component I-4 provides a substance having a vitamin B3 function, component I-5 provides a substance having a vitamin B5 function, component I-6 provides a substance having a vitamin B6 function, component I-7 provides a substance having a vitamin B9 function, component I-8 provides a substance having a vitamin B12 function, component I-9 provides a substance having a vitamin C function, component I-10 provides a substance having a vitamin D function, component I-11 provides a substance having a vitamin E function, and component I-12 provides a substance having a vitamin H function; each 1g of the vitamin premix comprises a component I-1, a component I-2, a component I-3, a component I-4, a component I-5, a component I-6, a component I-7, a component I-8, a component I-9, a component I-10, a component I-11 and a component I-12 which are 1100-1650 mu g, 14.22-21.33mg, 7.112-10.67mg, 49.11-73.67mg, 17.33-26.00mg, 7.112-10.67mg, 888.9-1333 mu g, 14.22-21.33 mu g, 255.5-383.3mg, 25.55-38.33 mu g, 49.12-73.68mg and 121.12-181.68 mu g in sequence;
the mineral premix comprises the following 9 effective components: component II-1, component II-2, component II-3, component II-4, component II-5, component II-6, component II-7, component II-8 and component II-9; the component II-1 provides a sodium element, the component II-2 provides a phosphorus element, the component II-3 provides a potassium element, the component II-4 provides a magnesium element, the component II-5 provides a calcium element, the component II-6 provides an iron element, the component II-7 provides a zinc element, the component II-8 provides a selenium element, and the component II-9 provides an iodine element; each 1g of the mineral premix contains sodium provided by the component II-1, phosphorus provided by the component II-2, potassium provided by the component II-3, magnesium provided by the component II-4, calcium provided by the component II-5, iron provided by the component II-6, zinc provided by the component II-7, selenium provided by the component II-8 and iodine provided by the component II-9 in sequence, wherein the contents of the sodium, the phosphorus, the potassium, the magnesium, the calcium, the iron, the zinc, the selenium and the iodine are 85.19-127.8mg, 40.92-61.39mg, 85.18-127.8mg, 13.08-19.61mg, 35.19-52.78mg, 1.2088-1.8134mg, 1.4226-2.134mg, 2.208-3.312 mug and 3.614-5.420 mug;
in the product, hydroxyproline, proline, glutamic acid, theanine, L-leucine, L-isoleucine and L-valine are sequentially mixed according to the mass ratio: 0.7: 0.9: 3.6: 0.07: 2.13: 1.12: 1.12;
the function of the product is as follows (a2) or (a 3):
(a2) preparing a functional beverage to be taken after exercise;
(a3) can be used as functional beverage for patients after exercise.
4. A method of making a product comprising the steps of:
(1) mixing vitamin premix, mineral premix, tea amino acid and taurine to obtain a mixed material A;
(2) mixing the mixture A, soybean protein isolate powder, branched chain amino acid, resistant dextrin, sodium carboxymethylcellulose, fructo-oligosaccharide, collagen, whey protein powder, hydrolyzed whey protein peptide, whole milk powder, crystalline fructose and wheat protein to obtain a product;
the weight ratio of wheat protein, whole milk powder, hydrolyzed whey protein peptide, whey protein powder, soybean protein isolate powder, collagen, branched chain amino acid, tea amino acid, crystalline fructose, fructo-oligosaccharide, sodium carboxymethylcellulose, resistant dextrin, taurine, vitamin premix and mineral premix is 6.37: 4.5: 2.15: 1.5: 1.5: 1.43: 2.8: 0.28: 5.07: 0.9: 0.8: 1.72: 0.04: 0.13: 0.81;
the vitamin premix comprises the following 12 effective components: the component I-1, the component I-2, the component I-3, the component I-4, the component I-5, the component I-6, the component I-7, the component I-8, the component I-9, the component I-10, the component I-11 and the component I-12; component I-1 provides a substance having a vitamin A function, component I-2 provides a substance having a vitamin B1 function, component I-3 provides a substance having a vitamin B2 function, component I-4 provides a substance having a vitamin B3 function, component I-5 provides a substance having a vitamin B5 function, component I-6 provides a substance having a vitamin B6 function, component I-7 provides a substance having a vitamin B9 function, component I-8 provides a substance having a vitamin B12 function, component I-9 provides a substance having a vitamin C function, component I-10 provides a substance having a vitamin D function, component I-11 provides a substance having a vitamin E function, and component I-12 provides a substance having a vitamin H function; each 1g of the vitamin premix comprises a component I-1, a component I-2, a component I-3, a component I-4, a component I-5, a component I-6, a component I-7, a component I-8, a component I-9, a component I-10, a component I-11 and a component I-12 which are 1100-1650 mu g, 14.22-21.33mg, 7.112-10.67mg, 49.11-73.67mg, 17.33-26.00mg, 7.112-10.67mg, 888.9-1333 mu g, 14.22-21.33 mu g, 255.5-383.3mg, 25.55-38.33 mu g, 49.12-73.68mg and 121.12-181.68 mu g in sequence;
the mineral premix comprises the following 9 effective components: component II-1, component II-2, component II-3, component II-4, component II-5, component II-6, component II-7, component II-8 and component II-9; the component II-1 provides a sodium element, the component II-2 provides a phosphorus element, the component II-3 provides a potassium element, the component II-4 provides a magnesium element, the component II-5 provides a calcium element, the component II-6 provides an iron element, the component II-7 provides a zinc element, the component II-8 provides a selenium element, and the component II-9 provides an iodine element; each 1g of the mineral premix contains sodium provided by the component II-1, phosphorus provided by the component II-2, potassium provided by the component II-3, magnesium provided by the component II-4, calcium provided by the component II-5, iron provided by the component II-6, zinc provided by the component II-7, selenium provided by the component II-8 and iodine provided by the component II-9 in sequence, wherein the contents of the sodium, the phosphorus, the potassium, the magnesium, the calcium, the iron, the zinc, the selenium and the iodine are 85.19-127.8mg, 40.92-61.39mg, 85.18-127.8mg, 13.08-19.61mg, 35.19-52.78mg, 1.2088-1.8134mg, 1.4226-2.134mg, 2.208-3.312 mug and 3.614-5.420 mug;
in the product, hydroxyproline, proline, glutamic acid, theanine, L-leucine, L-isoleucine and L-valine are sequentially mixed according to the mass ratio: 0.7: 0.9: 3.6: 0.07: 2.13: 1.12: 1.12;
the function of the product is as follows (a2) or (a 3):
(a2) preparing a functional beverage to be taken after exercise;
(a3) can be used as functional beverage for patients after exercise.
5. The product of the process of claim 4.
6. Use of the composition of claim 1 or the product of claim 2 or 3 or 5 as (b1) or (b 2):
(b1) preparing a functional beverage to be taken after exercise;
(b2) can be used as functional beverage for patients after exercise.
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