CN108534896A - Femtosecond time resolution pumps and the two-in-one spectrometer system of broadband time-resolved CARS - Google Patents

Femtosecond time resolution pumps and the two-in-one spectrometer system of broadband time-resolved CARS Download PDF

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Publication number
CN108534896A
CN108534896A CN201810186285.0A CN201810186285A CN108534896A CN 108534896 A CN108534896 A CN 108534896A CN 201810186285 A CN201810186285 A CN 201810186285A CN 108534896 A CN108534896 A CN 108534896A
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light
spectrometer
laser
time resolution
broadband
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CN108534896B (en
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张新海
刘双龙
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Shenzhen Peng Xing Photoelectric Technology Co Ltd
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Shenzhen Peng Xing Photoelectric Technology Co Ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28Investigating the spectrum
    • G01J3/2889Rapid scan spectrometers; Time resolved spectrometry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28Investigating the spectrum
    • G01J3/42Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry

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  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The invention discloses a kind of pumping of femtosecond time resolution and the two-in-one spectrometer systems of broadband time-resolved CARS, including optical flat, first light source entrance, second light source entrance, the first light path system, the second light path system and spectral detection system.The present invention relates to spectrometer technical fields, a kind of pumping of femtosecond time resolution and the two-in-one spectrometer system of broadband time-resolved CARS, utilize optical flat and various optical components, realize the modularization of the spectrometer, it is at low cost, it is simple in structure, femtosecond time resolution transient state absorption spectrometer and broadband time-resolved CARS spectrometer are combined, more convenient operating personnel are adjusted, easy to spread and application.

Description

Femtosecond time resolution pumps and the two-in-one spectrometer system of broadband time-resolved CARS
Technical field
The present invention relates to spectrometer technical field more particularly to a kind of pumpings of femtosecond time resolution and broadband time resolution The two-in-one spectrometer systems of CARS.
Background technology
Femtosecond time resolution transient state absorption spectrometer is pumped sample molecule to excitation state using pumping light pulse, passes through note The detection light then reached is recorded by the intensity variation before and after sample, come when studying the optical parameter for the sample that is excited with delay Between the rule that changes.Femtosecond time resolution transient absorption spectroscopy is research substance excited state energy level structure and excitation state energy The powerful tool of power relaxation process has a wide range of applications in fields such as physics, chemistry, material and biologies, such as optical physics Process, photochemical reaction, Biochemical processes, light-catalyzed reaction etc..
CARS spectrometers by the frequency of newborn spectrum component in recording light and material molecule interaction process, polarization, The information such as intensity carry out the molecular structure and its property of reactive scattering substance, thus can be in the feelings without introducing exogenous marker Under condition, sample molecule is effectively identified and positioned.Further, broad band CARS spectrometer can excite sample point simultaneously Multiple even all Raman oscillating bonds in son to obtain its complete CAR spectral information, and then are unfolded thin to live body The research of the structure and function of intracellular variety classes biomolecule etc., this hair to life science, Cell. Mol Exhibition is of great significance.
Existing femtosecond time resolution pump probe spectrometer and broadband time-resolved CARS spectrometer are all independent light System, thus need individually to be equipped with corresponding laser spectra system and build corresponding optical system.And photophysical process, In the researchs such as photochemical reaction and biomedicine, it can be commonly used and arrive femtosecond time resolution transient state absorption spectrometer and broadband time CARS spectrometers are differentiated, build femtosecond time resolution pump probe spectrometer and broadband time-resolved CARS spectrometer at this time, no It only needs the experimenter as profession to carry out operation and maintenance, and system expensive, huge, complicated, is unfavorable for the suction of femtosecond transient state Receive the promotion and application of spectral technique and broad band CARS spectral technique.
Invention content
In order to solve the above-mentioned technical problem, the object of the present invention is to provide a kind of femtosecond times at low cost, simple in structure Differentiate pumping and the two-in-one spectrometer system of broadband time-resolved CARS, easy to spread and application.
The technical solution adopted in the present invention is:A kind of pumping of femtosecond time resolution and broadband time-resolved CARS are two-in-one Spectrometer system, including optical flat, first light source entrance, second light source entrance, the first light path system, the second light path system and Spectral detection system,
All optical components in the spectrometer system are all placed on the optical flat;
First light path system, including the first plane mirror, second plane mirror, the first half-wave plate, the first lens, light Photonic crystal fiber, the second lens, third plane mirror, prism to, promote mirror, beam splitter and the 5th plane mirror;
The first light source entrance passes through the first plane mirror and second as first laser entrance, the first laser After plane mirror reflection, by the first half-wave plate, one end of the photonic crystal fiber, the light are converged to by the first lens The first laser is converted into super continuous spectrums laser by photonic crystal fiber, and the super continuous spectrums laser is by the photonic crystal fiber The other end outgoing, exported to prism pair by the second collimated, by prism to be output to promoted mirror to promote light beam, institute It states and promotes light beam and again pass by prism pair and be transmitted to third plane mirror, reflex to beam splitter and be divided into the first reflected light and the One transmitted light;
Second light path system, including photoswitch, the 6th plane mirror, the 7th plane mirror, the second half-wave plate, light Deferred mount, the 8th plane mirror and dichroscope;The second light source entrance swashs as second laser entrance, described second After light is by photoswitch, the second half-wave plate is reflexed to via the 6th plane mirror and the 7th plane mirror, by the second half Enter light deferred mount after wave plate, the second laser being emitted by light deferred mount reflexes to two by the 8th plane mirror To Look mirror;
The spectrometer system further includes the first object lens and the second object lens, and first transmitted light is via the 5th plane Speculum reflexes to dichroscope, and by being reached on sample to be tested after dichroscope and the first object lens, the second laser is passed through Dichroscope reflect, then by the first object lens reach sample to be tested on, the dichroscope be used for will first transmitted light with The second laser carries out conjunction beam, and second object lens collect the laser by sample to be tested;
Spectral detection system, including filtering apparatus, spectrometer, the first adapter, the second adapter, first conduction optical fiber and Second conduction optical fiber, the light beam that promoted are passed by the first reflected light that the beam splitter reflects via the first adapter and first Guiding fiber reaches spectrometer, and second object lens are fitted by filtering apparatus injection second again after collecting the laser by sample to be tested Orchestration reaches spectrometer via the second adapter and the second conduction optical fiber;
The filtering apparatus includes changeable long wave pass filter group and short wave pass filter group, when using the logical filter of long wave When mating plate group filters, the signal of spectrometer collection is femtosecond time resolution transient absorption spectra, when using short wave pass filter group When, the signal of spectrometer collection is broadband time-resolved CARS spectrum.
As being further improved for said program, the light tablet is stainless steel material or duralumin material.
As being further improved for said program, first lens are aspherical mirror.
As being further improved for said program, second lens are broadband achromatic doublet.
As being further improved for said program, the photonic crystal fiber is Totally positive dispersion photonic crystal fiber.
As being further improved for said program, the prism is to for dispersion compensation prism pair, the prism is to including putting down The first prism and the second prism that row is placed.
As being further improved for said program, the filtering apparatus further includes optical filter runner, the optical filter runner On the long wave pass filter group and short wave pass filter group are installed, filter for switching long wave pass filter group and short-pass Piece group enters thang-kng position.
As being further improved for said program, the light deferred mount includes moving horizontally platform and two are mutually perpendicular to Plane mirror.
As being further improved for said program, when spectrometer system pumps spectrometer as femtosecond time resolution, institute It states first laser and is converted into super continuous spectrums laser as detection light, when spectrometer system is as wide via the photonic crystal fiber When band time-resolved CARS spectrometer, the first laser is converted into super continuous spectrums laser conduct via the photonic crystal fiber Pump light and stokes light.
As being further improved for said program, when spectrometer system pumps spectrometer as femtosecond time resolution, institute Second laser is stated as pump light, when spectrometer system is as broadband time-resolved CARS spectrometer, the second laser is made To detect light.
The beneficial effects of the invention are as follows:
A kind of pumping of femtosecond time resolution and the two-in-one spectrometer system of broadband time-resolved CARS, using optical flat and Various optical components realize the modularization of the spectrometer, at low cost, simple in structure, by femtosecond time resolution transient absorption light Spectrometer and broadband time-resolved CARS spectrometer are combined, and are more convenient operating personnel and are adjusted, easy to spread and application.
Description of the drawings
The specific implementation mode of the present invention is described further below in conjunction with the accompanying drawings:
Fig. 1 is a kind of femtosecond time resolution pumping of the present invention and the two-in-one spectrometer system structure of broadband time-resolved CARS Schematic diagram;
Fig. 2 is that femtosecond of the present invention differentiates pumping spectrometer and broadband time-resolved CARS spectrometer switching schematic diagram.
Wherein, 1- optical flats;2- first light source entrances;3- second light source entrances;4- samples to be tested;The first planes of M1- Speculum;M2- second plane mirrors;M3- third plane mirrors;M4- promotes mirror;The 5th plane mirrors of M5-;M6- Six plane mirrors;The 7th plane mirrors of M7-;The 8th plane mirrors of M8-;The first half-wave plates of H1-;The second half-wave plates of H2-; The first lens of L1-;The second lens of L2-;A1- photonic crystal fibers;P1- prisms pair;BS- beam splitters;DM- dichroscopes;O1- One object lens;The second object lens of O2-;OS photoswitches;Delay- light deferred mounts;FW- filtering apparatus;The first adapters of D1-;D2- Second adapter;F1- first conducts optical fiber;F2- second conducts optical fiber;Spec- spectrometers.
Specific implementation mode
It should be noted that in the absence of conflict, the features in the embodiments and the embodiments of the present application can phase Mutually combination.
Fig. 1 is a kind of femtosecond time resolution pumping of the present invention and the two-in-one spectrometer system structure of broadband time-resolved CARS Schematic diagram, referring to Fig.1, a kind of pumping of femtosecond time resolution and the two-in-one spectrometer system of broadband time-resolved CARS, including light Tablet 1, first light source entrance 2, second light source entrance 3, the first light path system, the second light path system and spectral detection system are learned, Wherein, all optical components in the spectrometer system are all placed on optical flat 1, and are all installed to determining position, are made Modularization, in the present embodiment, optical flat 1 is stainless steel material or duralumin material.
Specifically, the first light path system includes the first plane mirror M1, second plane mirror M2, the first half-wave plate H1, the first lens L1, photon crystal optics A1, the second lens L2, third plane mirror M3, prism to P1, promoted mirror M4, point Beam mirror BS and the 5th plane mirror M5, first light source entrance 1 are used as first laser entrance, the first plane mirror M1 and second Plane mirror M2 is mutually parallel placement, and with horizontal plane angle at 45 °, first laser uses femto-second laser pulse, and first swashs Light passes through the first half-wave plate H1, the first half-wave plate H1 after the first plane mirror M1 and second plane mirror M2 reflections Then polarization state for adjusting incident first laser converges to one end of photon crystal optics A1 by the first lens L1, this In embodiment, the first lens L1 is aspherical mirror, for incident first laser to be focused and is injected into photonic crystal fiber A1, Photonic crystal fiber A1 is Totally positive dispersion photonic crystal fiber, and the super continuous spectrums laser of laser is composed when having preferable for generating, First laser is converted into super continuous spectrums laser by photonic crystal fiber A1, and super continuous spectrums laser is another by photonic crystal fiber A2's It brings out and penetrates, by the second lens L2 collimation output to prism to P1, in the present embodiment, the second lens L2 is the double glue of broadband achromatism Close lens, the super continuous spectrums laser generated for collimating photonic crystal fiber A2, P1 is output to by prism promoted mirror M4 with Light beam is promoted, light beam is promoted and again passes by prism to P1 and be transmitted to third plane mirror M3, then reflex to beam splitter BS It is divided into the first reflected light and the first transmitted light.
In the present embodiment, it is preferred that prism is dispersion compensation prism pair to P1, includes the first prism of the placement that is mutually parallel With the second prism, prism is used to carry out dispersion compensation to super continuous spectrums laser to P1.
Specifically, the second light path system, including photoswitch OS, the 6th plane mirror M6, the 7th plane mirror M7, Two half-wave plate H2, light deferred mount Delay, the 8th plane mirror M8 and dichroscope DM, wherein second light source entrance 3 As second laser entrance, second laser uses the femto-second laser pulse of tunable wave length, after second laser is by photoswitch OS, The second half-wave plate H2 is reflexed to via the 6th plane mirror M6 and the 7th plane mirror M7, it is laggard by the second half-wave plate H2 Enter light deferred mount Delay, by the second laser of light deferred mount Delay outgoing by the 8th plane mirror M8 reflections To dichroscope DM, wherein the 8th plane mirror M8 and dichroscope DM is mutually perpendicular to, and dichroscope DM and the 8th plane are anti- Penetrate mirror M8 with horizontal plane angle at 45 °, in the present embodiment, specifically, light deferred mount Delay includes moving horizontally platform and two A orthogonal plane mirror or hollow retroreflector, two plane mirrors with horizontal plane angle at 45 °.
The spectrometer system further includes the first object lens O1 and the second object lens O2, the 5th plane mirror M5 and beam splitter BS phases It is mutually placed in parallel, with horizontal plane angle at 45 °, dichroscope DM and the 5th plane are anti-by the 5th plane mirror M5 and beam splitter BS It penetrates mirror M5 to be mutually perpendicular to, the first transmitted light reflexes to dichroscope DM via the 5th plane mirror M5, then passes through dichroscope It is reached on sample to be tested 4 with the first object lens O1;Second laser is waited for by dichroscope DM reflections, then by the first object lens O1 arrival In sample 4, dichroscope DM is used to the first transmitted light and second laser carrying out conjunction beam, keeps two light beams spatially completely heavy It closes, the second object lens collect the laser by sample to be tested 4.
Specifically, light detecting system, including filtering apparatus FW, spectrometer Spec, the first adapter D1, the second adapter D2, the first conduction optical fiber F1 and the second conduction optical fiber F2 promote the first reflected light that light beam is reflected by beam splitter BS via the The conduction optical fiber of one adapter D1 and first F1 reaches spectrometer Spec, after the second object lens O2 collects the laser by sample to be tested 4, The second adapter D2 is injected by filtering apparatus FW, spectrometer is reached via the conduction optical fiber of the second adapter D2 and second F2 Spec.In the present embodiment, filtering apparatus FW includes changeable long wave pass filter group and short wave pass filter group, the logical filter of long wave Mating plate group is for filtering out femtosecond pulse, and short wave pass filter group is for filtering out femtosecond pulse and super continuous spectrums;When adopting When being filtered with long wave pass filter group, the signal of spectrometer Spec acquisitions is femtosecond time resolution transient absorption spectra, works as use When short wave pass filter group, the signal of spectrometer Spec acquisitions is broadband time-resolved CARS spectrum.Specifically, filtering apparatus FW Further include optical filter runner, long wave pass filter group and short wave pass filter group is installed, for switching length on optical filter runner Wave pass filter group and short wave pass filter group enter thang-kng position.
Fig. 2 is that femtosecond of the present invention differentiates pumping spectrometer and broadband time-resolved CARS spectrometer switching schematic diagram, reference Fig. 2, when spectrometer system pumps spectrometer as femtosecond time resolution, photoswitch work, odd number laser pulse is made to pass through, Even number laser pulse is blocked, for improving the signal-to-noise ratio of transient absorption spectra.First laser uses femto-second laser pulse, flies Second, laser pulse was converted into super continuous spectrums laser via Totally positive dispersion photonic crystal fiber, and super continuous spectrums laser is via prism to color After dissipating compensation deals, the first transmitted light and the first reflected light are divided by beam splitter, the first transmitted light is anti-as detection light, first It penetrates light to be used as with reference to light, second laser uses tunable femto-second laser pulse, and tunable femto-second laser pulse is as pump light, light Wire delay device drives two orthogonal reflectors or hollow retroreflector to be moved to the left, and pump light and detection light is made to generate Regular hour Δ t delay (i.e. second laser reaches sample to be tested after first reaching sample to be tested, super continuous spectrums laser), by waiting for Detection light after sample is by the long wave pass filter filtering pump light on optical filter runner, the femtosecond time resolution wink of generation State absorption spectrum signal is by spectrometer detection.
When spectrometer system is as broadband time-resolved CARS spectrometer, first laser uses femto-second laser pulse, flies Second, laser pulse was converted into super continuous spectrums laser via Totally positive dispersion photonic crystal fiber, and super continuous spectrums laser is via prism dispersion Pump light and stokes light are used as after compensation deals simultaneously, second laser uses tunable femto-second laser pulse, tunable to fly Second laser pulse drives two orthogonal reflectors or hollow retroreflector to move right as detection light, light deferred mount Dynamic, (i.e. super continuous spectrums laser first reaches generation regular hour Δ t delay between making pump light, stokes light and detecting light Sample to be tested is reached after sample to be tested, second laser), by the broadband time-resolved CARS signal after sample to be tested via second Object lens filter out super continuous spectrums laser using the short wave pass filter on optical filter runner after collecting and detect light, the broadband time point Distinguish CARS signals by spectrometer detection.
A kind of pumping of femtosecond time resolution and the two-in-one spectrometer system of broadband time-resolved CARS, using optical flat and Various optical components realize the modularization of the spectrometer, at low cost, simple in structure, by femtosecond time resolution transient absorption light Spectrometer and broadband time-resolved CARS spectrometer are combined, and are more convenient operating personnel and are adjusted, easy to spread and application.
It is to be illustrated to the preferable implementation of the present invention, but the invention is not limited to the implementation above Example, those skilled in the art can also make various equivalent variations or be replaced under the premise of without prejudice to spirit of that invention It changes, these equivalent deformations or replacement are all contained in the application claim limited range.

Claims (10)

1. a kind of femtosecond time resolution pumping and the two-in-one spectrometer system of broadband time-resolved CARS, which is characterized in that including Optical flat, first light source entrance, second light source entrance, the first light path system, the second light path system and spectral detection system, institute All optical components stated in spectrometer system are all placed on the optical flat;
First light path system, including the first plane mirror, second plane mirror, the first half-wave plate, the first lens, photon are brilliant Body optical fiber, the second lens, third plane mirror, prism to, promote mirror, beam splitter and the 5th plane mirror;
The first light source entrance passes through the first plane mirror and the second plane as first laser entrance, the first laser After speculum reflection, by the first half-wave plate, one end of the photonic crystal fiber is converged to by the first lens, the photon is brilliant The first laser is converted into super continuous spectrums laser by body optical fiber, and the super continuous spectrums laser is by the another of the photonic crystal fiber One end is emitted, and is exported to prism pair by the second collimated,
Mirror is promoted to promote light beam to being output to by prism, and the promotion light beam again passes by prism pair and is transmitted to third and puts down Face speculum reflexes to beam splitter and is divided into the first reflected light and the first transmitted light;
Second light path system, including the delay of photoswitch, the 6th plane mirror, the 7th plane mirror, the second half-wave plate, light Device, the 8th plane mirror and dichroscope;
The second light source entrance is as second laser entrance, anti-via the 6th plane after the second laser is by photoswitch It penetrates mirror and the 7th plane mirror reflexes to the second half-wave plate, by entering light deferred mount after the second half-wave plate, by light The second laser of deferred mount outgoing reflexes to dichroscope by the 8th plane mirror;
The spectrometer system further includes the first object lens and the second object lens, and first transmitted light is via the 5th plane reflection Mirror reflexes to dichroscope, by after dichroscope and the first object lens reach sample to be tested on, the second laser by two to Look mirror reflects, then is reached on sample to be tested by the first object lens, and the dichroscope is used for first transmitted light and described Second laser carries out conjunction beam, and second object lens collect the laser by sample to be tested;
Spectral detection system, including filtering apparatus, spectrometer, the first adapter, the second adapter, the first conduction optical fiber and second Optical fiber is conducted, the light beam that promoted conducts light by the first reflected light that the beam splitter reflects via the first adapter and first Fibre reaches spectrometer, and second object lens inject the second adaptation by filtering apparatus again after collecting the laser by sample to be tested Device reaches spectrometer via the second adapter and the second conduction optical fiber;
The filtering apparatus includes changeable long wave pass filter group and short wave pass filter group, when using long wave pass filter When group filtering, the signal of spectrometer collection is femtosecond time resolution transient absorption spectra, when using short wave pass filter group, light The signal of spectrometer acquisition is broadband time-resolved CARS spectrum.
2. a kind of femtosecond time resolution pumping according to claim 1 and the two-in-one spectrometer system of broadband time-resolved CARS System, which is characterized in that the light tablet is stainless steel material or duralumin material.
3. a kind of femtosecond time resolution pumping according to claim 2 and the two-in-one spectrometer system of broadband time-resolved CARS System, which is characterized in that first lens are aspherical mirror.
4. a kind of femtosecond time resolution pumping according to claim 3 and the two-in-one spectrometer system of broadband time-resolved CARS System, which is characterized in that second lens are broadband achromatic doublet.
5. a kind of femtosecond time resolution pumping according to claim 4 and the two-in-one spectrometer system of broadband time-resolved CARS System, which is characterized in that the photonic crystal fiber is Totally positive dispersion photonic crystal fiber.
6. a kind of femtosecond time resolution pumping according to claim 5 and the two-in-one spectrometer system of broadband time-resolved CARS System, which is characterized in that the prism is to for dispersion compensation prism pair, and the prism is to the first prism and the including being placed in parallel Two prisms.
7. a kind of femtosecond time resolution pumping according to claim 6 and the two-in-one spectrometer system of broadband time-resolved CARS System, which is characterized in that the filtering apparatus further includes optical filter runner, and the logical filter of the long wave is equipped on the optical filter runner Mating plate group and short wave pass filter group enter thang-kng position for switching long wave pass filter group and short wave pass filter group.
8. a kind of femtosecond time resolution pumping according to claim 7 and the two-in-one spectrometer system of broadband time-resolved CARS System, which is characterized in that the light deferred mount includes moving horizontally platform and two orthogonal plane mirrors.
9. being closed according to a kind of femtosecond time resolution pumping of claim 1 to 8 any one of them and broadband time-resolved CARS two One spectrometer system, which is characterized in that when spectrometer system pumps spectrometer as femtosecond time resolution, the first laser Super continuous spectrums laser is converted into as detection light, when spectrometer system is as broadband time resolution via the photonic crystal fiber When CARS spectrometers, the first laser is converted into super continuous spectrums laser as pump light and this via the photonic crystal fiber Lentor light.
10. two-in-one according to a kind of femtosecond time resolution pumping of claim 9 any one of them and broadband time-resolved CARS Spectrometer system, which is characterized in that when spectrometer system pumps spectrometer as femtosecond time resolution, the second laser is made For pump light, when spectrometer system is as broadband time-resolved CARS spectrometer, the second laser is as detection light.
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