CN108531643A - A kind of RAPD primers and its application for identifying variety of watermelon Soviet Union No. 6 seed purities of honey - Google Patents
A kind of RAPD primers and its application for identifying variety of watermelon Soviet Union No. 6 seed purities of honey Download PDFInfo
- Publication number
- CN108531643A CN108531643A CN201810709237.5A CN201810709237A CN108531643A CN 108531643 A CN108531643 A CN 108531643A CN 201810709237 A CN201810709237 A CN 201810709237A CN 108531643 A CN108531643 A CN 108531643A
- Authority
- CN
- China
- Prior art keywords
- soviet union
- watermelon
- sweet
- primer
- jaasrp1059
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of RAPD primers for identifying sweet No. 6 seed purities of variety of watermelon Soviet Union, including primer JAASRP1059 and JAASCR1036, and the sequence of primer JAASRP1059 is 5'GTTGCCAGCC 3'；The sequence of primer JAASCR1036 is 5'GTGACGTAGG 3'.Sweet No. 6 seed purities of Soviet Union, first extraction Soviet Union No. 6 DNA of honey can be identified using the RAPD primers, and using RAPD primer JAASRP1059 and JAASCR1036, PCR amplification is carried out using hybrid water melon Soviet Union No. 6 seed cdna group DNA of honey as template；Amplified production is detected into row agarose gel electrophoresis.The position difference of the polymorphism amplified fragments and electrophoresis pattern that are formed by comparing sequence dna fragment difference can identify sweet No. 6 genetic purities of Soviet Union, and qualification result fast and stable is reliable, and simple to operate.
Description
Technical field
The present invention relates to a kind of RAPD primers and its application for identifying variety of watermelon seed purity, especially a kind of use
RAPD primers in identification variety of watermelon Soviet Union No. 6 seed purities of honey and its application, belong to technical field of crop propagation.
Background technology
Watermelon is Curcurbitaceae hois spp annual herb plant, is important worldwide garden crop.China is watermelon life
Big country is produced, cultivated area and yield rank first in the world.New water melon breed has been achieved with 100% on domestic market
Cenospecies, watermelon seed quality and the development of watermelon industry are closely related, also directly affect the economic benefit of melon growers.But in west
During melon hybrid seeds, due to insect or wind pollination etc., the seed of maternal system's selfing is caused often to mix in F1In, it leads
It causes seed quality unqualified, huge economic loss is caused to enterprise and peasant household.Therefore, variety of watermelon seed purity is to grower
For be it is essential to ensure that.
Variety of watermelon ' Soviet Union's honey 6 ' is academy of agricultural sciences of Jiangsu Province Vegetable Research so self-mating system 20-207 is female parent, with selfing
It is the first generation of hybrid watermelon that 20-WS-3-1 is formulated for male parent.Precocity, fruit development period 30d；Plant growing way is medium, resistance to low
Warm dim light, bearing fruit property are strong；The high spheroidal of fruit, pericarp background color is blackish green, covers bottle green reticulate pattern, pericarp thickness 0.9cm, single melon quality
3.3kg；Center soluble solid content 11.8%, melon pulp pink, flesh matter is crisp, and fiber content is few, moisture foot, taste flavor
It is good；General 667m2Yield about 3500kg；Make Early-maturing culture suitable for East China.To ensure that it is maximum that improved seeds generate
Economic benefit, need it is a kind of quickly, detection method that is accurate, stablizing carry out the identification of variety of watermelon ' Soviet Union's honey 6 '.
Currently, traditional watermelon hybrid seed Purity method is mainly by field trapping test, foundation varietY specificity
(DUS) it tests, i.e., is judged by testing specificity, consistency and the stability of kind, and the big portion of character of watermelon DUS tests
Point all it is the quantitative character by controlled by multiple genes, is easily influenced by cultivation condition and weather conditions, and required time is longer, is tested
It is unstable result, time-consuming.
Invention content
It is an object of the invention to for the identification method that solves prior art variety of watermelon seed purity, time-consuming, time-consuming
Arduously, the problem of stability difference provides a kind of RAPD primers for identifying sweet No. 6 seed purities of variety of watermelon Soviet Union, utilizes
Its sweet No. 6 seed purity of energy Rapid identification variety of watermelon Soviet Union, qualification result is reliable and stable, simple to operate.
Technical solution
A kind of RAPD primers for identifying sweet No. 6 seed purities of variety of watermelon Soviet Union, including primer JAASRP1059 and draw
Object JAASCR1036, the nucleotides sequence of the primer JAASRP1059 are classified as 5'-GTTGCCAGCC-3'；The primer
The nucleotides sequence of JAASCR1036 is classified as 5'-GTGACGTAGG-3'.
Application of the above-mentioned primer in terms of sweet No. 6 seed purities of identification variety of watermelon Soviet Union.The application includes the following steps：
Application process：
(1) genomic DNA of extraction variety of watermelon Soviet Union honey 6；
(2) using variety of watermelon Soviet Union No. 6 genomic DNAs of honey as template, be utilized respectively primer JAASRP1059 and
JAASCR1036 carries out PCR amplification, obtains amplified production；
(3) amplified production is detected into row agarose gel electrophoresis；
(4) electrophoresis result is analyzed, as a contrast with the specific mark of Parent sample DNA, is only had simultaneously
The single plant of parent's specific mark just can be identified as sweet No. 6 cenospecies of Soviet Union, be otherwise hybrid.
Further, in step (1), the extracting method of genomic DNA is：It is taken when variety of watermelon revives sweet No. 6 two leaves wholeheartedly
Young leaflet tablet 1.5-3.5g, through liquid nitrogen grinding, with CTAB lysates (2%CTAB, 2M NaCl, 20mM EDTA, 100mM
Tris-HCl (pH=8.0) and 0.2%-mercaptoethanol) be transferred in 1.5mL centrifuge tubes, it is taken out after 65 DEG C of 0.5~1h of water-bath cold
But, it is 24 that isometric volume ratio, which is added,:1 chloroform and the mixture of isoamyl alcohol, after jiggling 20~30min, 12000r/
Min centrifuges 8~10min, and it is 24 to take supernatant volume ratio:1 chloroform and the mixture of isoamyl alcohol extract 1~2 time, are added
The isopropanol of 10% sodium acetate and precooling, 4 DEG C of refrigerators stand 3h or more, collect cotton-shaped DNA, dry after being washed with 70% ethyl alcohol,
Being dissolved in TE, (1mL 1mol/L TrisHcl (PH8.0), 0.2mL 0.5mol/L EDTA (PH8.0), add ddH2O is settled to
In 100mL), 4 DEG C save backup.
In step (2), the PCR amplification by RAPD primers JAASRP1059 and JAASCR1036 be divided to two groups be carried out at the same time or
It successively carries out, in addition to the primer of use is different, reaction system and response procedures are all identical.Further, in step (2), PCR amplification
Reaction system be 18uL, wherein containing：20ng genomic DNAs, 2.0mmolL-1MgCl2, 0.15mmolL-1DNTPs,
0.44umol·L-110 RAPD primers JAASRP1059 or JAASCR1036,0.75U Taq archaeal dna polymerases.
Further, in step (2), the response procedures of PCR amplification are：94 DEG C of 2min, 94 DEG C of 30s, 37 DEG C of 45s, 72 DEG C
90s, 40 cycles, 72 DEG C of 10min extend.
Further, in step (3), the voltage of agarose gel electrophoresis detection is 120V, electrophoresis time 0.5-1h.
Further, in step (4), the specific mark of the Parent sample DNA refers to：Utilize RAPD primers
When JAASRP1059 is expanded, the maternal specific mark that size is respectively 350bp and 450bp is generated, RAPD primers are utilized
When JAASCR1036 is expanded, the male parent specific mark that size is respectively 875bp and 1800bp is generated, only has parent special simultaneously
The single plant of heterolabeling is just sweet No. 6 cenospecies of really reviving.
Advantageous effect：The present invention provides the RAPD primers for identifying sweet No. 6 seed purities of variety of watermelon Soviet Union, and utilize
It carries out PCR amplification, by differentiating that ' the presence or absence of Parent specific band, calculates seed to variety of watermelon in Soviet Union's honey 6 ' single plant
Genetic purity.The detection method of the present invention has the advantages that the quality control for being fast and accurately beneficial to improve watermelon seed
Process.
Description of the drawings
Fig. 1 is the electrophoretogram of variety of watermelon Soviet Union honey 6 and its DNA fragmentation of parent, and wherein Fig. 1 a draw using RAPD
Object JAASRP1059, Fig. 1 b is using RAPD primers JAASCR1036.
Specific implementation mode
The invention will be further described in the following with reference to the drawings and specific embodiments.
Embodiment 1
A kind of RAPD primers for identifying sweet No. 6 seed purities of variety of watermelon Soviet Union, including primer JAASRP1059 and draw
Object JAASCR1036, the nucleotides sequence of the primer JAASRP1059 are classified as 5'-GTTGCCAGCC-3'；The primer
The nucleotides sequence of JAASCR1036 is classified as 5'-GTGACGTAGG-3'.Primer is synthesized by the bio tech ltd Qing Ke.
Using above-mentioned primer in the method for sweet No. 6 seed purities of identification variety of watermelon Soviet Union, include the following steps：
(1) genomic DNA of extraction variety of watermelon Soviet Union honey 6；
The extracting method of genomic DNA is：The extracting method of genomic DNA is：Sweet No. 6 two leaves are revived in variety of watermelon wholeheartedly
When take young leaflet tablet 2g, through liquid nitrogen grinding, with CTAB lysates (2%CTAB, 2M NaCl, 20mM EDTA, 100mM Tris-
HCl (pH=8.0) and 0.2%-mercaptoethanol) be transferred in 1.5mL centrifuge tubes, cooling is taken out after 65 DEG C of 0.5~1h of water-bath, is added
It is 24 to enter isometric volume ratio:1 chloroform and the mixture of isoamyl alcohol, after jiggling 20~30min, 12000r/min from
8~10min of the heart, it is 24 to take supernatant volume ratio:1 chloroform and the mixture of isoamyl alcohol extract 1~2 time, and 10% vinegar is added
The isopropanol of sour sodium and precooling, 4 DEG C of refrigerators stand 3h or more, collect cotton-shaped DNA, dry after being washed with 70% ethyl alcohol, are dissolved in TE
(1mL 1mol/L TrisHcl (PH8.0), 0.2mL 0.5mol/L EDTA (PH8.0), add ddH2O is settled to 100mL)
In, 4 DEG C save backup.
(2) it is utilized respectively RAPD primer JAASRP1059 and JAASCR1036, with No. 6 genomes of variety of watermelon Soviet Union honey
DNA is template, carries out PCR amplification on 2720 thermal cyclers of American AB I, obtains amplified production；
Pcr amplification reaction system is 18uL, wherein containing：20ng genomic DNAs, 2.0mmolL-1MgCl2,
0.15mmol·L-1DNTPs, 0.44umolL-110RAPD primers JAASRP1059 or JAASCR1036,0.75U Taq DNA
Polymerase (TAKARA)；
Pcr amplification reaction program is：94 DEG C of 2min, 94 DEG C of 30s, 37 DEG C of 45s, 72 DEG C of 90s, 40 cycles, 72 DEG C of 10min
Extend, after in 4 DEG C preservation.
(3) amplified production is detected into row agarose gel electrophoresis；
According to DNA quantitatively detect as a result, using H2It is spare to 200ng/ul that O dilutes pcr amplification product.TAE running buffers
Liquid：50 × TAE electrophoretic buffers：12.2g Tris, 2.85mL glacial acetic acid, 10ml 0.25mol/L EDTA (PH8.0) add water
To 50mL.
Using electrophoretic buffer as solvent, 1g agaroses are weighed, add 50 × TAE electrophoretic buffer 2mL and 98mL distilled water,
It is dissolved on micro-wave oven, is made into 1% Ago-Gel 100mL.After slightly cold, 0.5ug/mLGoldView, mixing is added；It installs
Electrophoresis tank and sample comb, encapsulating；After glue cooling, 1 × TAE electrophoretic buffers are poured into electrophoresis tank.Connection electrode, in 120V perseverances
Press strip part carries out electrophoresis, and electrophoresis time is 0.5~1h, until the DNA bands of amplification are sufficiently spread out.
It observes and takes pictures on Ultraviolet Detector, the result is shown in Figure 1, Fig. 1 a are variety of watermelon Soviet Union sweet No. 6 and its parent's DNA fragmentations
RAPD primer JAASRP1059 electrophoretograms, Fig. 1 b be variety of watermelon Soviet Union honey No. 6 and its parent's DNA fragmentation RAPD primers
JAASCR1036 electrophoretograms, 1：It is maternal；2：Male parent；3：Sweet No. 6 cenospecies of Soviet Union；M is DNAmarker.
(4) electrophoresis result is analyzed, as a contrast with the specific mark of Parent sample DNA, is only had simultaneously
The single plant of parent's specific mark just can be identified as sweet No. 6 cenospecies of Soviet Union, be otherwise hybrid.
Claims (8)
1. a kind of RAPD primers for identifying sweet No. 6 seed purities of variety of watermelon Soviet Union, which is characterized in that including primer
JAASRP1059 and primer JAASCR1036, the nucleotides sequence of the primer JAASRP1059 are classified as 5'-GTTGCCAGCC-3'；
The nucleotides sequence of the primer JAASCR1036 is classified as 5'-GTGACGTAGG-3'.
2. application of the RAPD primers described in claim 1 in terms of sweet No. 6 seed purities of identification variety of watermelon Soviet Union.
3. application as claimed in claim 2, which is characterized in that include the following steps：
(1) genomic DNA of extraction variety of watermelon Soviet Union honey 6；
(2) using sweet No. 6 genomic DNAs of variety of watermelon Soviet Union as template, it is utilized respectively primer JAASRP1059 and JAASCR1036,
PCR amplification is carried out, amplified production is obtained；
(3) amplified production is detected into row agarose gel electrophoresis；
(4) electrophoresis result is analyzed, as a contrast with the specific mark of Parent sample DNA, only there is parent simultaneously
The single plant of specific mark just can be identified as sweet No. 6 cenospecies of Soviet Union, be otherwise hybrid.
4. application as claimed in claim 3, which is characterized in that in step (1), the extracting method of genomic DNA is：In watermelon
Kind takes 1.5~3.5g of young leaflet tablet when reviving sweet No. 6 two leaves wholeheartedly, and through liquid nitrogen grinding, 1.5mL centrifugations are transferred to CTAB lysates
Cooling is taken out after Guan Zhong, 65 DEG C of 0.5~1h of water-bath, it is 24 that isometric volume ratio, which is added,:The mixing of 1 chloroform and isoamyl alcohol
Object, after jiggling 20~30min, 12000r/min centrifuges 8~10min, and it is 24 to take supernatant volume ratio:1 chloroform with
The mixture of isoamyl alcohol extracts 1~2 time, and the isopropanol of 10% sodium acetate and precooling is added, and 4 DEG C of refrigerators stand 3h or more, collect
Cotton-shaped DNA, it is dry after being washed with 70% ethyl alcohol, it is dissolved in TE, 4 DEG C save backup.
5. application as claimed in claim 3, which is characterized in that in step (2), the reaction system of PCR amplification is 18uL, wherein
Contain：20ng genomic DNAs, 2.0mmolL-1MgCl2, 0.15mmolL-1DNTPs, 0.44umolL-110RAPD primers
JAASRP1059 or JAASCR1036,0.75U Taq archaeal dna polymerases.
6. application as claimed in claim 3, which is characterized in that in step (2), the response procedures of PCR amplification are：94℃
2min, 94 DEG C of 30s, 37 DEG C of 45s, 72 DEG C of 90s, 40 cycles, 72 DEG C of 10min extend.
7. application as claimed in claim 3, which is characterized in that in step (3), the voltage of agarose gel electrophoresis detection is
120V, electrophoresis time 0.5-1h.
8. such as claim 3 to 7 any one of them application, which is characterized in that in step (4), the Parent sample DNA
Specific mark refers to：When being expanded using RAPD primers JAASRP1059, the female parent spy that size is respectively 350bp and 450bp is generated
Heterolabeling when being expanded using RAPD primers JAASCR1036, is generated the male parent that size is respectively 875bp and 1800bp and specifically marked
Note, it is just sweet No. 6 cenospecies of really reviving only to have the single plant of parent's specific mark simultaneously.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810709237.5A CN108531643B (en) | 2018-07-02 | 2018-07-02 | RAPD primer for identifying purity of watermelon variety Sumi No. 6 seeds and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810709237.5A CN108531643B (en) | 2018-07-02 | 2018-07-02 | RAPD primer for identifying purity of watermelon variety Sumi No. 6 seeds and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108531643A true CN108531643A (en) | 2018-09-14 |
| CN108531643B CN108531643B (en) | 2020-09-08 |
Family
ID=63487621
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810709237.5A Active CN108531643B (en) | 2018-07-02 | 2018-07-02 | RAPD primer for identifying purity of watermelon variety Sumi No. 6 seeds and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108531643B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109234437A (en) * | 2018-10-31 | 2019-01-18 | 宁夏泰金种业股份有限公司 | A kind of watermelon hybrid seed Purity method based on SSR marker method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102220315A (en) * | 2011-04-15 | 2011-10-19 | 北京市农林科学院 | Watermelon complete genomic sequence information based analyzed and developed SSR core primer combinations and application thereof |
| CN102732621A (en) * | 2012-06-12 | 2012-10-17 | 南京农业大学 | Rapid identification method of genetic purity of muskmelon hybrid seeds based on PCR |
| CN104059992A (en) * | 2014-07-14 | 2014-09-24 | 天津科润农业科技股份有限公司 | Method for identifying seed purity of muskmelon hybrid variety based on EST-SSR (expressed sequence tag-simple sequence repeat) marker |
| KR101693410B1 (en) * | 2015-01-09 | 2017-01-17 | 충북대학교 산학협력단 | Random Amplified Polymorphic DNA primer for discrimination of Zizyphus jujuba Miller |
-
2018
- 2018-07-02 CN CN201810709237.5A patent/CN108531643B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102220315A (en) * | 2011-04-15 | 2011-10-19 | 北京市农林科学院 | Watermelon complete genomic sequence information based analyzed and developed SSR core primer combinations and application thereof |
| CN102732621A (en) * | 2012-06-12 | 2012-10-17 | 南京农业大学 | Rapid identification method of genetic purity of muskmelon hybrid seeds based on PCR |
| CN104059992A (en) * | 2014-07-14 | 2014-09-24 | 天津科润农业科技股份有限公司 | Method for identifying seed purity of muskmelon hybrid variety based on EST-SSR (expressed sequence tag-simple sequence repeat) marker |
| KR101693410B1 (en) * | 2015-01-09 | 2017-01-17 | 충북대학교 산학협력단 | Random Amplified Polymorphic DNA primer for discrimination of Zizyphus jujuba Miller |
Non-Patent Citations (1)
| Title |
|---|
| 王春晖等: "基于ＩＳＳＲ和ＲＡＰＤ标记的八株灰树花栽培菌株遗传多样性分析", 《食用菌学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109234437A (en) * | 2018-10-31 | 2019-01-18 | 宁夏泰金种业股份有限公司 | A kind of watermelon hybrid seed Purity method based on SSR marker method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108531643B (en) | 2020-09-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103993011B (en) | Semen Sesami dominant genic male sterility gene molecule marker and preparation method and application | |
| CN104342434B (en) | The method for identifying molecules of cotton cells matter male sterile restoring line | |
| CN104561284B (en) | The molecular assay method of cotton zero formula fruit branch gene | |
| CN106916897A (en) | One kind is used to identify the molecular labeling and its application of giant pumpkin ' silver-colored brightness three ' hybrid seed purity | |
| CN110016521B (en) | Molecular marking method for rapidly identifying bud leaf early-growing tea tree germplasm | |
| CN108531643A (en) | A kind of RAPD primers and its application for identifying variety of watermelon Soviet Union No. 6 seed purities of honey | |
| CN113604598A (en) | Molecular marker primer and method for identifying common camellia oleifera and small camellia oleifera | |
| CN109735648A (en) | A kind of method and its dedicated kit for screening different mass of 1000 kernel wheats | |
| CN109207628B (en) | Molecular marker suitable for detecting purple radishes and application | |
| WO2012068772A1 (en) | Pcr identification method for germplasm materials of upland cotton hb red flower | |
| CN105483225B (en) | The specific Function molecular labeling of rice temp-sensing genic male sterile gene tms5 and its application | |
| CN108165652B (en) | Specific molecular marker TGMI001 for identifying sex of torreya grandis at seedling stage | |
| CN116144821A (en) | Identification method of tea-oil camellia self-incompatibility female determinant S gene and kit matched with same | |
| CN103468805B (en) | Marker and method for identifying cytoplasmic sterility homozygous restorer line of Gossypium harknessii | |
| CN112746124B (en) | SSR primer and method for purity identification of cauliflower hybrid seeds | |
| CN108707645A (en) | A kind of identification method of the sweet ninth-seeded purity of variety of watermelon Soviet Union | |
| CN104263719B (en) | Oat seed DNA large-scale extraction and purification method suitable for PCR amplification | |
| CN109055599B (en) | Molecular marker primer for identifying Jinmei variety of kiwi fruit and application thereof | |
| CN113215269A (en) | Detection kit for visual detection of potato rot stem nematode and application thereof | |
| CN110747262A (en) | Millet under drought stress H2Method for detecting change of methylation level of DNA after S treatment | |
| CN114634991B (en) | InDel marker for identifying high-variety coconuts and application thereof | |
| CN104017801A (en) | Micro-extraction method for extracting DNA (Deoxyribonucleic Acid) from single pepper seed | |
| CN111286554A (en) | SSR primer for identifying purity of hybrid seeds of Niubao white gourd and application of SSR primer | |
| CN106244683B (en) | Primer for detecting " Qiong Li " small watermelon purity of hybrid combines and its methods and applications | |
| CN113897454B (en) | PCR-based rapid identification method for tomato wilt |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |