CN108523143A - Black tiger palm dietary fiber extract is preparing treatment and/or is preventing the purposes during high fat diet causes the preparation of hepatic injury relevant disease - Google Patents
Black tiger palm dietary fiber extract is preparing treatment and/or is preventing the purposes during high fat diet causes the preparation of hepatic injury relevant disease Download PDFInfo
- Publication number
- CN108523143A CN108523143A CN201810210282.6A CN201810210282A CN108523143A CN 108523143 A CN108523143 A CN 108523143A CN 201810210282 A CN201810210282 A CN 201810210282A CN 108523143 A CN108523143 A CN 108523143A
- Authority
- CN
- China
- Prior art keywords
- dietary fiber
- preparation
- ethyl alcohol
- black tiger
- tiger palm
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000284 extract Substances 0.000 title claims abstract description 47
- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 43
- 241000569924 Pinanga maculata Species 0.000 title claims abstract description 42
- 238000002360 preparation method Methods 0.000 title claims abstract description 31
- 201000010099 disease Diseases 0.000 title claims abstract description 23
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 23
- 235000009200 high fat diet Nutrition 0.000 title claims abstract description 21
- 231100000753 hepatic injury Toxicity 0.000 title claims abstract description 19
- 239000003814 drug Substances 0.000 claims abstract description 8
- 229940079593 drug Drugs 0.000 claims abstract description 7
- 230000036541 health Effects 0.000 claims abstract description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 71
- 235000019441 ethanol Nutrition 0.000 claims description 41
- 238000010992 reflux Methods 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 12
- 238000000605 extraction Methods 0.000 claims description 12
- 238000010828 elution Methods 0.000 claims description 10
- 238000009835 boiling Methods 0.000 claims description 8
- 239000000047 product Substances 0.000 claims description 8
- 239000004365 Protease Substances 0.000 claims description 7
- 239000003153 chemical reaction reagent Substances 0.000 claims description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 6
- 230000002265 prevention Effects 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 206010019708 Hepatic steatosis Diseases 0.000 claims description 5
- 108090000526 Papain Proteins 0.000 claims description 5
- 229940088598 enzyme Drugs 0.000 claims description 5
- 235000013305 food Nutrition 0.000 claims description 5
- 229940055729 papain Drugs 0.000 claims description 5
- 235000019834 papain Nutrition 0.000 claims description 5
- 238000001556 precipitation Methods 0.000 claims description 5
- 206010019663 Hepatic failure Diseases 0.000 claims description 4
- 241000282376 Panthera tigris Species 0.000 claims description 4
- 238000001976 enzyme digestion Methods 0.000 claims description 4
- 238000012869 ethanol precipitation Methods 0.000 claims description 4
- 231100000835 liver failure Toxicity 0.000 claims description 4
- 208000007903 liver failure Diseases 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 208000004930 Fatty Liver Diseases 0.000 claims description 3
- 206010067125 Liver injury Diseases 0.000 claims description 3
- 208000034189 Sclerosis Diseases 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 230000002440 hepatic effect Effects 0.000 claims description 3
- 208000008338 non-alcoholic fatty liver disease Diseases 0.000 claims description 3
- 206010053219 non-alcoholic steatohepatitis Diseases 0.000 claims description 3
- -1 95% ethyl alcohol Chemical compound 0.000 claims description 2
- 108091005804 Peptidases Proteins 0.000 claims description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 2
- 239000003937 drug carrier Substances 0.000 claims description 2
- 230000002255 enzymatic effect Effects 0.000 claims description 2
- 208000010706 fatty liver disease Diseases 0.000 claims description 2
- 239000002244 precipitate Substances 0.000 claims description 2
- 230000001376 precipitating effect Effects 0.000 claims description 2
- 235000019419 proteases Nutrition 0.000 claims description 2
- 231100000240 steatosis hepatitis Toxicity 0.000 claims description 2
- 244000189799 Asimina triloba Species 0.000 claims 1
- 235000006264 Asimina triloba Nutrition 0.000 claims 1
- 235000009467 Carica papaya Nutrition 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 206010061218 Inflammation Diseases 0.000 abstract description 6
- 230000002757 inflammatory effect Effects 0.000 abstract description 6
- 230000004054 inflammatory process Effects 0.000 abstract description 6
- 230000006866 deterioration Effects 0.000 abstract description 3
- 230000003449 preventive effect Effects 0.000 abstract description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 22
- 230000000968 intestinal effect Effects 0.000 description 20
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 18
- 238000012360 testing method Methods 0.000 description 17
- 208000008589 Obesity Diseases 0.000 description 12
- 210000004185 liver Anatomy 0.000 description 12
- 235000020824 obesity Nutrition 0.000 description 12
- 241000699670 Mus sp. Species 0.000 description 11
- 241001465754 Metazoa Species 0.000 description 9
- 235000012000 cholesterol Nutrition 0.000 description 9
- 235000005911 diet Nutrition 0.000 description 9
- 230000037213 diet Effects 0.000 description 8
- 238000011160 research Methods 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 210000002966 serum Anatomy 0.000 description 6
- 210000000813 small intestine Anatomy 0.000 description 6
- 150000003626 triacylglycerols Chemical class 0.000 description 6
- 241000605059 Bacteroidetes Species 0.000 description 5
- 241000192125 Firmicutes Species 0.000 description 5
- 241000233866 Fungi Species 0.000 description 5
- 108010023302 HDL Cholesterol Proteins 0.000 description 5
- 108010028554 LDL Cholesterol Proteins 0.000 description 5
- 230000008520 organization Effects 0.000 description 5
- 230000001575 pathological effect Effects 0.000 description 5
- 238000011084 recovery Methods 0.000 description 5
- 102100036475 Alanine aminotransferase 1 Human genes 0.000 description 4
- 108010082126 Alanine transaminase Proteins 0.000 description 4
- 102000009027 Albumins Human genes 0.000 description 4
- 108010088751 Albumins Proteins 0.000 description 4
- 108010003415 Aspartate Aminotransferases Proteins 0.000 description 4
- 102000004625 Aspartate Aminotransferases Human genes 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 244000005709 gut microbiome Species 0.000 description 4
- 210000005229 liver cell Anatomy 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 241000186000 Bifidobacterium Species 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 230000003908 liver function Effects 0.000 description 3
- 235000012054 meals Nutrition 0.000 description 3
- 241000702460 Akkermansia Species 0.000 description 2
- 241000731710 Allobaculum Species 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000304886 Bacilli Species 0.000 description 2
- 241001143779 Dorea Species 0.000 description 2
- 108010002350 Interleukin-2 Proteins 0.000 description 2
- 108090001005 Interleukin-6 Proteins 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 238000008050 Total Bilirubin Reagent Methods 0.000 description 2
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 2
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 2
- 238000000246 agarose gel electrophoresis Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 210000005252 bulbus oculi Anatomy 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000003304 gavage Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000008595 infiltration Effects 0.000 description 2
- 238000001764 infiltration Methods 0.000 description 2
- 210000004969 inflammatory cell Anatomy 0.000 description 2
- 108010074109 interleukin-22 Proteins 0.000 description 2
- 229940039696 lactobacillus Drugs 0.000 description 2
- 208000019423 liver disease Diseases 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 244000005706 microflora Species 0.000 description 2
- 238000001543 one-way ANOVA Methods 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 1
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 241000305071 Enterobacterales Species 0.000 description 1
- 241000194033 Enterococcus Species 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010020880 Hypertrophy Diseases 0.000 description 1
- 238000008214 LDL Cholesterol Methods 0.000 description 1
- 108010007622 LDL Lipoproteins Proteins 0.000 description 1
- 102000007330 LDL Lipoproteins Human genes 0.000 description 1
- NPGIHFRTRXVWOY-UHFFFAOYSA-N Oil red O Chemical compound Cc1ccc(C)c(c1)N=Nc1cc(C)c(cc1C)N=Nc1c(O)ccc2ccccc12 NPGIHFRTRXVWOY-UHFFFAOYSA-N 0.000 description 1
- ZYFVNVRFVHJEIU-UHFFFAOYSA-N PicoGreen Chemical compound CN(C)CCCN(CCCN(C)C)C1=CC(=CC2=[N+](C3=CC=CC=C3S2)C)C2=CC=CC=C2N1C1=CC=CC=C1 ZYFVNVRFVHJEIU-UHFFFAOYSA-N 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 241000577937 Sarcodon imbricatus Species 0.000 description 1
- YIQKLZYTHXTDDT-UHFFFAOYSA-H Sirius red F3B Chemical compound C1=CC(=CC=C1N=NC2=CC(=C(C=C2)N=NC3=C(C=C4C=C(C=CC4=C3[O-])NC(=O)NC5=CC6=CC(=C(C(=C6C=C5)[O-])N=NC7=C(C=C(C=C7)N=NC8=CC=C(C=C8)S(=O)(=O)[O-])S(=O)(=O)[O-])S(=O)(=O)O)S(=O)(=O)O)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+] YIQKLZYTHXTDDT-UHFFFAOYSA-H 0.000 description 1
- 244000273618 Sphenoclea zeylanica Species 0.000 description 1
- 240000008866 Ziziphus nummularia Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000002547 anomalous effect Effects 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 238000010201 enrichment analysis Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 231100000234 hepatic damage Toxicity 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000008818 liver damage Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 230000013370 mutualism Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000021590 normal diet Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000002040 relaxant effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
- A23L33/22—Comminuted fibrous parts of plants, e.g. bagasse or pulp
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Mycology (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The purposes of purposes more particularly to a kind of black brave palm dietary fiber extract in preparing treatment and/or preventing the preparation of high fat diet cause hepatic injury relevant disease that the present invention relates to black tiger palm dietary fiber extracts in pharmaceutical field.The black tiger palm dietary fiber extract of the present invention can not only be clearly helpful for the reparation that high fat diet causes hepatic injury, but also pair with the relevant disease of hepatic injury show significantly treat and/or preventive effect, such as it can effectively inhibit the expression of inflammatory factor, the deterioration of inflammation is effectively relieved, improve immune-related every physiopathological index, it can be used in the various fields such as drug, health products, there is great economy and social value.
Description
Technical field
The present invention relates to black tigers to slap purposes of the dietary fiber extract in the fields such as pharmacy, health products and health food,
More particularly to a kind of black tiger palm dietary fiber extract causes hepatic injury relevant disease in preparation treatment and/or prevention high fat diet
Purposes in preparation.
Background technology
Obesity refers to that body fat mass in human body is excessively accumulated, hence it is evident that exceed normal person average amount, and occur organism metabolism,
The anomalous variation of Physiology and biochemistry, is referred to as obesity.By 2014, the world had 600,000,000 Genus Homos in obese people, it is contemplated that 2025
Year, world's obesity number will reach 1,400,000,000 people, and problem of obesity has become one of the public health problem that the world attractes attention.Research
It was found that the occurrence and development majority of affluent society common disease and fat related such as diabetes, hypertension, coronary heart disease, hepatopathy, colon
Cancer, kidney trouble, Alzheimer's disease etc..Fat mechanism is complicated, mainly including inherent cause, environmental factor etc., wherein
Most of obese patients belong to simple obesity, and caused by unreasonable diet structure, high fat diet intake is excessive, dietary fiber
Insufficiency of intake is to lead to one of fat occurrence factor.
Enteric microorganism group is known as " second set of genome of human body ", closely bound up with human health.The micro- life of human intestine
Species up to 1000-1150 kinds, wherein Firmicutes and Bacteroidetes are dominant microflora, the gene dosage in enteron aisle genome
It is more than 150 times of mankind's autogene group.There is mutualism relationships with host for intestinal flora, and dynamic is remain in enteron aisle
Balance, when balance is broken, people is possible to suffer from various diseases.Existing research confirms:(1) intestinal flora and fat phase
It closes, there is the phenomenon that intestinal microflora imbalance, Phylogenetic diversity of bacteria declines more in obese people;The Bacteroidetes of obesity mice
Few, Firmicutes are more;The enteric microorganism of obesity mice is transplanted on germfree mouse, the latter's fat mass can be caused to increase.It is right
For fat people, high fat diet is taken in for a long time, the change of intestinal microflora, unbalance intestinal flora can be caused to will produce
Endotoxin enters human body, and the inflammation factor is generated after being identified by immunocyte, body is caused to be in low grade inflammation state, generates
Metabolic disorder so that the energy absorbed from food is easily converted to fat, causes obesity;(2) liver and enteron aisle are in biology
Functionally it is closely related, interactional.Liver is interacted with intestinal flora by intestines-liver axis, intestinal flora and liver
Disease is close, when liver illness, can influence the structure and function of intestinal flora, and can be influenced in turn when intestinal bacilli illness
Liver function, the beneficial bacteriums quantity such as liver cirrhosis patient Bifidobacterium reduces, and the harmful bacterias quantity such as enterobacteria, enterococcus increases.
High fat diet can change intestinal microflora, and beneficial bacterium is made to reduce, and destroy the balance of intestinal flora, and then influence
Liver function, causes hepatic injury, and increase the problems such as with the development of the living standard in China and dietary structure, by high in fat
The incidence of hepatic injury caused by diet obviously increases, and there is an urgent need to develop preparing treatment and/or preventing high fat diet cause liver
Damage the preparation in terms of relevant disease.
HEIHUZHANG fungus (Saarcodon imbricatum (L.:Fr.) Karst.), mushroom cap is fleshy hypertrophy, gives off a strong fragrance, newly
Be canescence or taupe when fresh, become that dull gray is white or taupe after dry, shaped like brave pawl, lower surface cover with one layer it is very thin greyish white
Color thorn-like fine hair, how hollow stem is sturdy, and long is a little inclined.Black tiger palm field distributed area very little, China only has southwest partly
Area produces, very precious.
HEIHUZHANG fungus is considered as rare mountain delicacy very early in history, is one of successive dynasties' favorite tribute in successive dynasties imperial palace.Europe
It is that the black tiger palm is consumed mainly that beauteously area does not have food custom, Southwest China, Japan and one band of Southeast Asia to HEIHUZHANG fungus
Area.The bacterium flat property and sweet taste has the effect of wind-evil dispelling and cold-evil expelling, relaxing tendons and activating collaterals, civil to be also used as establishing-Yang with it, and having, which reduces courage in blood, consolidates
The effect of alcohol.
Invention content
The purpose of the present invention is to provide a kind of preparation methods of black tiger palm dietary fiber extract, and slap meals with black tiger
It eats fiber extract treatment and/or prevents high fat diet and cause hepatic injury preparation relevant disease.
In the first aspect of the present invention, it is high in preparation treatment and/or prevention to provide the black tiger palm dietary fiber extract of one kind
Fat diet causes the purposes in the preparation of hepatic injury relevant disease.
Wherein, the hepatic injury relevant disease includes that fatty liver, hepatic steatosis, nonalcoholic steatohepatitis, liver are hard
Change, hepatic failure.
Preferably, the black tiger palm dietary fiber extract is preparing treatment and/or prevention of hepatic steatosis, non-alcohol
Purposes in the preparations of diseases such as property fat hepatitis, hepatic sclerosis, hepatic failure.
In the second aspect of the present invention, a kind of preparation method of black tiger palm dietary fiber extract, including by sample comminution
Afterwards, it is extracted using alcohol reflux, filter residue uses water refluxing extraction, then digests, alcohol precipitation, elutes, and it is fine to finally obtain black tiger palm diet
Tie up extract.
Preferably, the alcohol reflux, which extracts, includes:Use solid-liquid ratio for 1:8-1:15 ethyl alcohol, refluxing extraction 2-4 are small
When.
Preferably, the filter residue includes using water refluxing extraction:Use solid-liquid ratio for 1:8-1:15 water, refluxing extraction 2-
4 hours.
Preferably, the enzymolysis is using papain enzymolysis.
Preferably, the enzymolysis dosage is 0.2% volume of extracting solution, and the enzymatic activity is 750,000 u/g-85, ten thousand u/g.
Preferably, the hydrolysis temperature is 50 DEG C -70 DEG C, and the time is 1-2 hours.
Preferably, described to be precipitated as precipitating using alcohol reagent, such as ethyl alcohol, methanol.
It is further preferred that described be precipitated as the ethyl alcohol that 4-6 times of liquid is measured after addition digests, precipitate 12-24 hours.
Preferably, the elution carries out gradient elution for the precipitation that will be obtained using ethyl alcohol, the acetone of increasing concen-trations.
It is further preferred that the elution, which is the precipitation that will be obtained, uses 78% ethyl alcohol, 95% ethyl alcohol, absolute ethyl alcohol, third
Ketone carries out gradient elution.
Preferably, the preparation method of the black tiger palm dietary fiber extract includes the following steps:
(1) it is 1 according to solid-liquid ratio after the black tiger palm is dry:8-1:Ethyl alcohol such as 95% ethyl alcohol is added in 15 (mass volume ratios),
2h-4h is extracted with ethyl alcohol boiling reflux, filters, obtains filter residue;
(2) it is 1 according to solid-liquid ratio:8-1:15 (mass volume ratios) are into filter residue plus water, boiling reflux extract 2h-4h;
(3) when temperature is down to 50 DEG C -60 DEG C or so, pH value is adjusted to 6-7, is added in the filter residue obtained to step (2)
Papain enzymolysis, enzyme amount are 0.2% weight percent, and 50-70 DEG C of water enzyme digestion 1h-2h is kept stirring;
(4) after digesting, the ethanol precipitation that 4-6 times of extracting liquid volume is measured is added and stays overnight, filters, obtains filter residue;
(5) filter residue is subjected to gradient elution using 78% ethyl alcohol, 95% ethyl alcohol, absolute ethyl alcohol, acetone, dries, crushes, it is excellent
80 mesh screens were selected, black tiger palm dietary fiber extract is obtained.
In a preferred embodiment, the preparation method of the black tiger palm dietary fiber extract includes the following steps:
(1) it is crushed after the dry such as drying of the black tiger palm, is 1 according to solid-liquid ratio:10 are added ethyl alcohol such as 95% ethyl alcohol, use second
Alcohol boiling reflux extracts 2h, filters, obtains filter residue;
(2) it is 1 according to solid-liquid ratio:10 into filter residue plus water, boiling reflux extract 2h;
(3) when temperature is down to 50 DEG C -60 DEG C or so, pH value is adjusted to 6-7, wood is added in the filter residue obtained to step 2
Melon protease hydrolyzed, enzyme amount are 0.2% weight percent, and 60 DEG C of water enzyme digestion 1h are kept stirring;
(4) after digesting, 95% ethanol precipitation that 4 times of amounts of extracting liquid volume are added is stayed overnight, and is filtered, is obtained filter residue;
(5) filter residue is subjected to gradient elution using 78% ethyl alcohol, 95% ethyl alcohol, absolute ethyl alcohol, acetone, dries, crushes, it is excellent
80 mesh screens were selected, black tiger palm dietary fiber is obtained.
The third aspect of the present invention, it is a kind for the treatment of and/or preventing liver injury relevant disease preparation, the preparation includes medicine
Object, health products or food, the preparation include that black tiger slaps dietary fiber extract.
In the fourth aspect of the present invention, a kind of drug prevented and/or treat hepatic injury relevant disease, including black tiger palm meals
Eat fiber extract and pharmaceutically acceptable carrier.
Preferably, the black tiger palm dietary fiber extract is the extract that above-mentioned preparation method is prepared.
Compared with prior art, the present invention has the advantages that:
(1) preparation method is simple, operating cost is low by the present invention, and the Extraction solvent that preparation method of the present invention uses is only
Use second alcohol and water, ethyl alcohol that can remain risk with recycling, no organic reagent, ensure that the natural quality of extract
It is simultaneously and environmentally friendly, belong to green manufacture technology.
(2) present invention removes alcohol-soluble substance, using in papain enzymolysis sample using the black tiger palm of ethyl alcohol extraction
Protein removes isolating protein, improves the recovery rate and purity of black tiger palm dietary fiber, greatly improves recovery rate and purity.
(3) black tiger palm dietary fiber extract of the invention can not only be clearly helpful for high fat diet and cause repairing for hepatic injury
It is multiple, but also pair show significantly to treat with the relevant disease of hepatic injury and/or preventive effect, such as can effectively inhibit inflammation
The deterioration of inflammation is effectively relieved in the expression of the factor, improves immune-related every physiopathological index, can be used for drug, protects
In the various fields such as strong product, there is great economy and social value.
Description of the drawings
Fig. 1 is the small intestine HE coloring pathological sections figure (observation of 200 power microscopes) of embodiment 2.
Fig. 2 is the liver organization pathological section figure (observation of 200 power microscopes) of embodiment 2.
It is further illustrated the present invention below in conjunction with specific implementation mode, but the scope of protection of present invention is not limited to
In following embodiments.
As used herein, term "comprising" or " comprising " refer to the element that method includes cited, but are not excluded for other
Element.
HEIHUZHANG fungus scientific name jujube Sarcodon imbricatus (L. Ex Franch.) Karst of the present invention is famous one of Export of Edible Fungi.Thalline is sturdy
Loose, fine and tender taste is not easy tattered and full of nutrition containing abundant exocellular polysaccharide.It is measured according to analysis, dry product contains 17 kinds of ammonia
Base acid, wherein having 7 kinds of essential amino acids for accounting for total amount 41.46% and 11 kinds of minerals and trace element.It is this edible
Bacterium value is high, has production, more than 1000 yuan one jin of price in Northern Guangdong Province.
Embodiment 1:The preparation method of black tiger palm dietary fiber extract
(1) it crushes and weighs after sample drying, solid-liquid ratio 1:10,2h is extracted with 95% ethyl alcohol boiling reflux, is filtered, is protected
Stay filter residue;
(2) filter residue adds water, solid-liquid ratio 1:10, boiling reflux extracts 2h;
(3) it when temperature is down to 60 DEG C or so, adjusts pH value to 6-7, papain enzymolysis is added, enzyme amount is sample weighting amount
During which 0.2%, 60 DEG C of water enzyme digestion 1h are kept stirring;
(4) enzymolysis terminates, and 95% ethanol precipitation that 4 times of amounts of extracting liquid volume are added is stayed overnight, and is filtered, and filter residue is retained;
(5) filter residue carries out gradient elution using 78% ethyl alcohol, 95% ethyl alcohol, absolute ethyl alcohol, acetone, dries, and crushes, mistake
80 mesh screens, are made dietary fiber.
Recovery rate is calculated for the black tiger palm dietary fiber extract being prepared, recovery rate is:Extract quality/sample
Sample weighting amount × 100%=49.55%.
The measurement of physicochemical property is carried out for the black tiger palm dietary fiber extract being prepared:Expansive force is 7.21ml/
G, expansion rate 7.12ml/ml, retention ability 5.98g/g, it is 0.63g/g to hold oily power, and absorption cholesterol ability is 34.21mg/g
(pH2.0)、 41.29mg/g(pH7.0)。
Constituent analysis is carried out for the black tiger palm dietary fiber extract being prepared:Moisture 4.98%, protein
6.85%, ash content 5.66%, total dietary fiber 84.37%.
Embodiment 2:Improvement result of the black tiger palm dietary fiber extract to high fat diet obesity-induced mice hepatic injury
1, reagent:
Total cholesterol (T-CHO) testing cassete:Bioengineering Research Institute is built up in Nanjing.
Triglycerides (TG) testing cassete:Bioengineering Research Institute is built up in Nanjing.
Low density lipoprotein cholesterol (LDL-C) testing cassete:Bioengineering Research Institute is built up in Nanjing.
High-density lipoprotein cholesterol (HDL-C) testing cassete:Bioengineering Research Institute is built up in Nanjing.
Alanine aminotransferase (ALT) testing cassete, aspartate amino transferase (AST) testing cassete, total protein
(TP) test, albumin (ALB) test and total bilirubin (T-Bil) testing cassete all build up bio-engineering research purchased from Nanjing
Institute.
Female C57 mouse 30, are purchased from Guangdong Province medical experiment animal by SPF grades, weight 12-14g, age in days 28-30d
Center, credit number are SCXK (Guangdong) 2013-0002.
Rats and mice maintains feed:Guangdong Medical Lab Animal Center, credit number SCXK (Guangdong) 2013-0002.
High cholesterol diet formula high in fat is as shown in table 1:
The main component and energy of 1 high cholesterol diet high in fat of table are constituted
Black tiger palm dietary fiber extract is prepared by experimental example 1 of the present invention.
2, method
Animal subject is raised in Institute of Micro-biology of Guangdong Province animal experimental center, raising temperature and humidity:23 ± 1 DEG C, 55 ±
10%, using 12h intermittent illuminations round the clock;Receptacle condition remains stable, with the reliability of guarantee test result.Mouse is certainly
It is drunk water by feed.Period daily check animal is primary, does not find unsound animal, and whole healthy mices is taken to be tested.30
C57 mouse are randomly divided into normal group, model group and administration group after adaptability raises 7d, every group 10.It is normal to organize with general
Logical feed forage feed, model group and administration group are fed with high cholesterol diet high in fat, continuous to feed one month.
After modeling success, start to be administered, administration group gives black tiger palm dietary fiber extract gavage, the extraction of 1g dietary fibers
Object is diluted with 15ml distilled water, the daily gavage 0.6ml of every mouse, successive administration 2 months.Normal group normal diet feed
It feeds, model group and administration group are fed with high cholesterol diet high in fat, continuous to feed one month.
3, result is observed
The appearance figure (hair, color, shape) of daily observation animal and dynamic simultaneously make respective record, and the weight of animals is measured every 3d
Once.
Blood:After being discontinued for 24 hours, animal materials testing index.Acquire blood sample, pluck eyeball and take blood, blood plasma through 3000RPM,
Serum is collected in 10min centrifugations twice, and it is horizontal to measure T-CHO, TG, LDL-C and HDL-C in serum.It includes thin to measure inflammatory factor
Intracellular cytokine interleukins IL-2, IL-6, IL-22, tumor necrosis factor TNF-alpha and Foxo3a.
Tissue:It plucks after eyeball takes blood, puts to death and dissect mouse, take mouse small intestine and liver organization, be fixed on 4% poly first
In aldehyde.Small intestine and liver organization are made into paraffin section, and small intestine is dyed using Hematoxylin-eosin (HE) method, liver organization
Hematoxylin-eosin (HE), Picro-Sirius red (SRS) and oil red O (ORS) dyeing is respectively adopted, carries out pathology section examination.
4, data processing
All data are with mean standard deviationIt indicates.The comparison of multigroup mean uses one-way analysis of variance
(One-Way ANOVA), mean compares two-by-two between group, and LSD methods are used when variance is neat;Dunnett ' s T3 are used when heterogeneity of variance
Method.It is completed by SPSS softwares, α=0.05.
It has been observed that the fur of the animal of administration group is more smoother than the fur of model group.
The changes of weight of each group is as shown in table 2.
2 mouse weight of table changes
#P<0.05,##P<0.01,###P<Normal group of 0.001vs;*P<0.05,**P<0.01,***P<0.001vs model groups
From table 2 it can be seen that normal average weight difference between group and model group extremely significantly (P<0.001), model
Group mouse average weight about 20.35g when starting, average weight is 35.29g at the end of experiment, illustrates that high fat diet induces
Mice model of obesity modeling success;Compared with model group, administration group average mice body weight is remarkably decreased (P<0.01) (table 2).This
Show:Suitable dietary fiber is added in high fat diet can significantly slow weight gain.
The four items of blood lipid tests testing result of each group is as shown in table 3.
3 mice serum biochemical indicator (mmol/L) of table
#P<0.05,##P<0.01,###P<Normal group of 0.001vs;*P<0.05,**P<0.01,***P<0.001vs model groups
From table 3 it can be seen that normally group is aobvious for total cholesterol, triglycerides and ldl-cholesterol ratio in model group
It writes and increases (P<0.001 or P<0.01), high-density lipoprotein cholesterol significantly reduces (P than normal group<0.001);With model group
It compares, triglycerides, total cholesterol, low-density lipoprotein cholesterol level are remarkably decreased (P in administration group<0.05 or P<
0.01), High-density Lipoprotein-cholesterol significantly increases (P<0.05).This shows:Suitable meals are added in high fat diet
Food fiber can significantly reduce total cholesterol, triglycerides and low-density lipoprotein white level in serum.
The inflammatory factor testing result of each group is as shown in table 4.
4 inflammatory factor testing result of table
#P<0.05,##P<0.01,###P<Normal group of 0.001vs;*P<0.05,**P<0.01,***P<0.001vs model groups
From table 4, it can be seen that compared with normal group, all cytokine levels deviate normally in model group serum,
TNF-α, IL-2, IL-6, IL-22, Foxo3 significant difference (p<0.05);Compared with model group, all these cells of administration group
The factor is obviously restored, to show that black tiger palm dietary fiber can improve inflammatory environment.
The results are shown in Table 5 for the liver functional testing of each group.
5 liver functional testing result of table
#P<0.05,##P<0.01,###P<Normal group of 0.001vs;*P<0.05,**P<0.01,***P<0.001vs model groups
As can be seen from Table 5, normal group of notable liter of TP, ALB, ALT, AST and T-BIL level ratio in model group mice serum
High P<0.05 or P<0.01;Compared with model group, administration group TP, ALB, ALT, AST and T-BIL level significantly reduces (P<
0.05).This shows:Suitable dietary fiber is added in high fat diet can significantly improve liver function.
The results are shown in Figure 1 for small intestine's HE coloring pathological sections, it will be seen from figure 1 that normally group small intestine villus
Long and complete, model group intestinal villus falls off seriously, illustrates that high fat diet can cause to damage to the enteron aisle of mouse, illustrates that model is
Successfully.Compared with model group, after drug-treated, administration group can alleviate damage, promote the reparation of enteron aisle.
The results are shown in Figure 2 for liver organization pathological section, figure it is seen that HE coloration results show normal group mouse
Liver cell structure is normal, and model group mouse liver cell volume increases, and occurs many circles not of uniform size in hepatocyte cell matter
Blown-out shot pushes nucleus to side, locally there is inflammatory cell infiltration phenomenon;Compared with model group, administration group mouse liver cell fat
Fat vacuole quantity significantly reduces, and inflammatory cell infiltration phenomenon is improved;SRS is dyed and ORS coloration results are shown respectively, with mould
Type group is compared, and administration group mouse liver cell structure is obviously improved, and tends to normal group.
Embodiment 3:Black tiger palm dietary fiber extract is to high fat diet obesity-induced mice intestinal bacilli illness correlation disease
The improvement result of disease
1, experimental method
Stool in mice is stored in -80 DEG C of ice after liquid nitrogen is rapidly frozen at the end of zoopery in acquisition embodiment 2
In case.DNA is extracted from fecal specimens, and DNA is detected by 0.8% agarose gel electrophoresis and extracts quality, while using purple
Outer spectrophotometer quantifies DNA.Using forward primer 5 '-ACTCCTACGGGAGGCAGCA-3 ' and reverse primer 5'-
GGACTACHVGGGTWTCTAAT-3' expands the areas the V3-V4 gene of mouse 16S rRNA.Pcr amplification product passes through 2%
Agarose gel electrophoresis is detected, and carries out gel extraction to target fragment, and recycling is recycled using the gel of AXYGEN companies
Kit.With reference to the preliminary quantitative result of electrophoresis, PCR amplification recovery product is subjected to fluorescent quantitation, fluorescent reagent Quant-iT
PicoGreen dsDNA Assay Kit, quantitative instrument are Microplate reader (BioTek, FLx800).According to fluorescence
Quantitative result is mixed each sample by corresponding proportion according to the sequencing amount demand of each sample.Using Illumina public affairs
The TruSeq Nano DNA LT Library Prep Kit of department prepare sequencing library.Using MiSeq sequenators carry out 2 ×
The both-end of 300bp is sequenced, and corresponding reagent is MiSeq Reagent Kit V3 (600cycles).
2, experimental result
The results are shown in Table 6 for the intestinal flora diversity of each group.
6 mouse intestinal flora diversity indices of table
#P<0.05,##P<0.01,###P<Normal group of 0.001vs;*P<0.05,**P<0.01,***P<0.001vs model groups
As can be seen from Table 6, compared with normal group, the Alpha diversity indices Simpson of model group mouse intestinal flora
It is remarkably decreased (P with Shannon<0.05), illustrate that high fat diet changes mouse intestinal flora structure, make the reduction of its diversity;
Compared with model group, administration group mouse intestinal flora diversity indices Simpson and Shannon significantly increase (P<0.05) it, says
After the obesity mice intestinal flora of bright high fat diet induction gives black tiger palm dietary fiber treatment, intestinal flora diversity significantly increases
Add.
OTU enrichment analysis is as shown in table 7 and table 8.
Abundance of 7 mouse intestinal flora of table in door level
#P<0.05,##P<0.01,###P<Normal group of 0.001vs;*P<0.05,**P<0.01,***P<0.001vs model groups
Abundance of 8 mouse intestinal flora of table on belonging to level
#P<0.05,##P<0.01,###P<Normal group of 0.001vs;*P<0.05,**P<0.01,***P<0.001vs model groups
As can be seen from Table 7, in door level, compared with normal group, model group mouse intestinal flora Bacteroidetes
Significant changes have occurred with Firmicutes abundance, Bacteroidetes abundance significantly reduces (P<0.001), Firmicutes
Abundance dramatically increases (P<0.001), compared with model group, administration group Bacteroidetes abundance dramatically increases (P<0.01),
Firmicutes abundance significantly reduces (P<0.01).
As can be seen from Table 8, on belonging to level, compared with normal group, model group beneficial bacterium Akkermansia,
Bifidobacterium and Lactobacillus significantly reduces (P<0.01), harmful bacteria Allobaculum (P<0.001) and
Dorea(P<0.05) dramatically increase, compared with model group, administration group beneficial bacterium Akkermansia, Bifidobacterium and
Lactobacillus dramatically increases (P<0.01), harmful bacteria Allobaculum (P<And Dorea (P 0.05)<0.05) it significantly drops
It is low.
To sum up it can be seen that, it is extensive that black tiger palm dietary fiber extract can not only be clearly helpful for dominant microflora structure in enteron aisle
It is multiple, contribute to flora diversified, but also pair show significantly to treat and/or prevent with the relevant disease of intestinal microflora
Effect can effectively inhibit the expression of inflammatory factor, and the deterioration of inflammation is effectively relieved, and improve immune-related every physiological and pathological and refer to
Mark, can be used in the various fields such as drug, health products, have great economy and social value.
The above, only preferable specific embodiment of the invention, but scope of protection of the present invention is not limited thereto,
Any one skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Design is subject to equivalent substitution or change, should all cover within the scope of the present invention.
Claims (10)
1. a kind of black tiger palm dietary fiber extract is preparing the system treated and/or prevented high fat diet and cause hepatic injury relevant disease
Purposes in agent.
2. purposes according to claim 1, which is characterized in that the hepatic injury relevant disease includes fatty liver, liver
Steatosis, nonalcoholic steatohepatitis, hepatic sclerosis, hepatic failure.
3. according to the purposes of claims 1 or 2, which is characterized in that the black tiger palm dietary fiber extract is preparing treatment
And/or the purposes in the preparation of the diseases such as prevention of hepatic steatosis, nonalcoholic steatohepatitis, hepatic sclerosis, hepatic failure.
4. a kind of preparation method of black tiger palm dietary fiber extract, which is characterized in that after crushing the black tiger palm, using second
Alcohol reflux extracts, and filter residue is used water refluxing extraction, then by extracting solution enzymolysis, precipitation, elution, obtain black tiger palm dietary fiber and carry
Take object.
5. preparation method according to claim 4, which is characterized in that the alcohol reflux, which extracts, includes:Using solid-liquid ratio
It is 1:8-1:15 ethyl alcohol, refluxing extraction 2-4 hours;And/or the filter residue includes using water refluxing extraction:Using solid-liquid ratio
It is 1:8-1:15 water, refluxing extraction 2-4 hours.
6. preparation method according to claim 4, which is characterized in that the enzymolysis is excellent for using papain enzymolysis
Choosing, the enzymolysis dosage is 0.2% volume of extracting solution, and the enzymatic activity is 750,000 u/g-85, ten thousand u/g;And/or the enzyme
It is 50 DEG C -70 DEG C to solve temperature, and the time is 1-2 hours.
7. preparation method according to claim 4, which is characterized in that it is described to be precipitated as precipitating using alcohol reagent, such as
Ethyl alcohol, methanol;And/or it is precipitated as the ethyl alcohol that 4-6 times of liquid is measured after addition digests, it precipitates 12-24 hours;And/or it described washes
It takes off for obtained precipitation is carried out gradient elution using 78% ethyl alcohol, 95% ethyl alcohol, absolute ethyl alcohol, acetone.
8. according to any preparation method in claim 4 to 7, include the following steps:
(1) it will be crushed after the dry such as drying of the black tiger palm, be 1 according to solid-liquid ratio:10 are added ethyl alcohol such as 95% ethyl alcohol, use ethyl alcohol
Boiling reflux extracts 2h, filters, obtains filter residue;
(2) it is 1 according to solid-liquid ratio:10 into filter residue plus water, boiling reflux extract 2 hours;
(3) when temperature is down to 50 DEG C -60 DEG C or so, pH value is adjusted to 6-7, pawpaw is added in the filter residue obtained to step (2)
Protease hydrolyzed, enzyme amount are 0.2% weight percent, and 60 DEG C of water enzyme digestions 1 hour are kept stirring;
(4) after digesting, 95% ethanol precipitation that 4 times of amounts of extracting liquid volume are added is stayed overnight, and is filtered, is obtained filter residue;
(5) filter residue is subjected to gradient elution using 78% ethyl alcohol, 95% ethyl alcohol, absolute ethyl alcohol, acetone, dries, crushes, preferably mistake
80 mesh screens obtain black tiger palm dietary fiber extract.
9. the preparation of a kind for the treatment of and/or preventing liver injury relevant disease, which is characterized in that the preparation includes drug, health care
Product or food, the preparation include that black tiger slaps dietary fiber extract.
10. the drug of a kind of prevention and/or treatment hepatic injury relevant disease, which is characterized in that carried comprising black tiger palm dietary fiber
Take object and pharmaceutically acceptable carrier.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810210282.6A CN108523143A (en) | 2018-03-14 | 2018-03-14 | Black tiger palm dietary fiber extract is preparing treatment and/or is preventing the purposes during high fat diet causes the preparation of hepatic injury relevant disease |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810210282.6A CN108523143A (en) | 2018-03-14 | 2018-03-14 | Black tiger palm dietary fiber extract is preparing treatment and/or is preventing the purposes during high fat diet causes the preparation of hepatic injury relevant disease |
Publications (1)
Publication Number | Publication Date |
---|---|
CN108523143A true CN108523143A (en) | 2018-09-14 |
Family
ID=63483510
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201810210282.6A Pending CN108523143A (en) | 2018-03-14 | 2018-03-14 | Black tiger palm dietary fiber extract is preparing treatment and/or is preventing the purposes during high fat diet causes the preparation of hepatic injury relevant disease |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN108523143A (en) |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106617080A (en) * | 2015-10-30 | 2017-05-10 | 无限极(中国)有限公司 | Application of lentinan in preparation of healthcare food with intestinal flora regulating function |
CN106883308A (en) * | 2017-03-27 | 2017-06-23 | 安徽大学 | Anti-tumor active polysaccharide SAP of gymnospermum nigricans fruiting body and preparation method and application thereof |
CN108379284A (en) * | 2018-03-14 | 2018-08-10 | 广东省微生物研究所(广东省微生物分析检测中心) | Purposes of the black tiger palm dietary fiber extract in preparing treatment and/or preventing intestinal bacilli illness relevant disease preparation |
-
2018
- 2018-03-14 CN CN201810210282.6A patent/CN108523143A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106617080A (en) * | 2015-10-30 | 2017-05-10 | 无限极(中国)有限公司 | Application of lentinan in preparation of healthcare food with intestinal flora regulating function |
CN106883308A (en) * | 2017-03-27 | 2017-06-23 | 安徽大学 | Anti-tumor active polysaccharide SAP of gymnospermum nigricans fruiting body and preparation method and application thereof |
CN108379284A (en) * | 2018-03-14 | 2018-08-10 | 广东省微生物研究所(广东省微生物分析检测中心) | Purposes of the black tiger palm dietary fiber extract in preparing treatment and/or preventing intestinal bacilli illness relevant disease preparation |
Non-Patent Citations (5)
Title |
---|
何国庆: "《食品发酵与酿造工艺学》", 31 August 2003, 中国农业出版社 * |
朱彩平: "酶法提取平菇多糖工艺研究", 《中成药》 * |
苏爱梅: "《食品营养与健康》", 31 January 2013, 中国质检出版社 * |
迟玉杰: "《保健食品学》", 31 May 2016, 中国轻工业出版社 * |
陈健: "黑虎掌菌多糖的组成和抗肿瘤活性", 《华南理工大学学报(自然科学版)》 * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN108142923A (en) | Purposes of the mushroom dietary fiber extract in the preparation for preparing treatment and/or prevention intestinal bacilli illness relevant disease | |
CN106962933B (en) | Application of nymphaea hybrid extract and composition thereof in preventing obesity and improving intestinal flora | |
CN105663444A (en) | Compound immunity-enhancing and aging-resisting agent and preparation method thereof | |
TWI484966B (en) | Extraction process for caulerpa lentillifera extract and pharmaceutical composition containing caulerpa lentillifera extract for inhibiting growth of prostate cancer cells | |
CN104523742B (en) | A kind of polysaccharide composition and its application with protect liver and raising immunization | |
CN109134591A (en) | A kind of balsam pear peptide extracting method and its application | |
CN108379286A (en) | Mushroom dietary fiber extract is preparing treatment and/or is preventing the purposes during high fat diet causes the preparation of hepatic injury relevant disease | |
Ma et al. | Inhibitory effect of fermented flammulina velutipes polysaccharides on mice intestinal inflammation | |
CN108379284A (en) | Purposes of the black tiger palm dietary fiber extract in preparing treatment and/or preventing intestinal bacilli illness relevant disease preparation | |
CN101356975B (en) | Health food capable of increasing human immunity and promoting intelligence and strengthening brain and preparation method thereof | |
CN108159090A (en) | Purposes of the lucidum spore powder dietary fiber extract in treatment and/or prevention intestinal bacilli illness relevant disease preparation is prepared | |
CN105012826B (en) | A kind of intelligence development leaf extract and preparation method thereof, application | |
CN112741851A (en) | Method for extracting sparrow tea extract by eutectic solvent method and preparation and application of granules | |
CN108379285A (en) | Ovum spore Aode mushroom dietary fiber extract is preparing treatment and/or is preventing the purposes during high fat diet causes the preparation of hepatic injury relevant disease | |
CN108523143A (en) | Black tiger palm dietary fiber extract is preparing treatment and/or is preventing the purposes during high fat diet causes the preparation of hepatic injury relevant disease | |
CN109568420A (en) | The purposes of Chinese wampee seed and its extract in the food, health care product or drug of preparation treatment diabetes, obesity and hyperlipidemia | |
CN116355816A (en) | Microorganism of fermented samara oil seed and blood lipid reducing composition thereof | |
CN101225422A (en) | Method for extracting polysaccharide from salvia miltiorrhizae | |
CN109771453A (en) | Purposes of the birch young pilose antler extract in the preparation of preparation treatment and/or prevention intestinal bacilli illness disease and related disease | |
CN109758485A (en) | Purposes of the russule extract in the preparation of preparation treatment and/or prevention intestinal bacilli illness disease and related disease | |
CN109771457A (en) | Purposes of the russule extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease | |
CN115895961A (en) | Blood pressure lowering probiotic powder and application thereof | |
CN101199564B (en) | Application of sanchi flower total saponine in preparing treatment hypertension disease medicament | |
CN108159089A (en) | Purposes of the lucidum spore powder dietary fiber extract in the preparation for preparing treatment and/or prevention high fat diet cause hepatic injury relevant disease | |
CN110496192A (en) | A kind of natural medicinal formulations for preventing and treating atherosclerosis by adjusting intestinal flora balance |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180914 |
|
RJ01 | Rejection of invention patent application after publication |