CN108503041B - Method for treating bean curd stick wastewater by using microbial battery - Google Patents
Method for treating bean curd stick wastewater by using microbial battery Download PDFInfo
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- CN108503041B CN108503041B CN201810303589.0A CN201810303589A CN108503041B CN 108503041 B CN108503041 B CN 108503041B CN 201810303589 A CN201810303589 A CN 201810303589A CN 108503041 B CN108503041 B CN 108503041B
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- curd stick
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/005—Combined electrochemical biological processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M8/00—Fuel cells; Manufacture thereof
- H01M8/16—Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Water Supply & Treatment (AREA)
- Hydrology & Water Resources (AREA)
- Organic Chemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Biodiversity & Conservation Biology (AREA)
- Electrochemistry (AREA)
- Biochemistry (AREA)
- Sustainable Energy (AREA)
- Sustainable Development (AREA)
- Manufacturing & Machinery (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Water Treatment By Electricity Or Magnetism (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a method for treating dried bean waste water, which comprises the steps of collecting river mud samples and separating microorganisms by adopting a screening culture medium. After cultivation, the rod-shaped microorganisms were selected. Culturing at 37 deg.C for 5 days, and inoculating to the bean curd stick wastewater at an inoculum size of 6-10%. And adding the inoculated dried beancurd stick wastewater into the anode of the fuel cell, and culturing at normal temperature for treatment. Through the treatment of the method, the COD (chemical oxygen demand) of the bean curd stick waste water is 16496mg/L, and after 20 days of culture, the lowest COD is measured to be 668 mg/L. The COD removal rate reaches 95.96%, and the COD can be directly discharged into the environment. The invention provides a method for treating bean curd stick wastewater.
Description
Technical Field
The invention relates to the field of food treatment, in particular to a method for treating bean curd stick wastewater.
Background
The waste water for producing the dried beancurd sticks mainly contains starch, protein, fat and the like, has extremely high concentration of organic matter content, and is solved by adopting a mode of taking physical chemistry as a main method, such as: adding chemicals to flocculate, then precipitating, or carrying out air flotation, but the method has the disadvantages of low treatment efficiency and high operation cost. The waste water of the dried beancurd sticks is extremely high in content of degradable organic matters and less in toxic and harmful substances, and is treated by a microbial method, so that the treatment efficiency is high, and part of energy is output in an electric form.
Disclosure of Invention
The invention aims to provide a method for treating bean curd stick wastewater.
The invention is realized by the following technical scheme:
the following reagents and consumables are required for treating the dried bean wastewater:
screening Medium
Glucose, peptone, beef extract, yeast powder, KCl, NaH2PO4·2H2O，Na2HPO4·12H2O。
Nfion117 proton semipermeable membrane
Biofuel cell structure
The cell was composed of two square glass containers connected by a flange in the middle, each container having a length of 8cm, a width of 8cm and a height of 8 cm. The middle interface is clamped with a proton semipermeable membrane and sealed by a rubber ring. One chamber is the anode and one chamber is the cathode. The dried bean curd stick waste water is added into the anode, and the deionized water is added into the cathode. During the operation of the cell, air is continuously introduced into the cathode chamber by an air compressor.
The treatment method comprises the following steps:
1. collecting river mud samples, putting the river mud samples into a sterilized collecting pipe, and culturing in an anaerobic incubator by adopting a screening culture medium.
2. After 1 week of anaerobic culture, the microorganisms were isolated. After microscopic examination, rod-shaped microorganisms are selected.
3. Anaerobic culture is carried out by adopting a screening culture medium to separate rod-shaped microorganisms, the rod-shaped microorganisms are cultured for 5 days at 37 ℃, and the rod-shaped microorganisms are inoculated into the bean curd stick wastewater by 6-10% of inoculation amount.
4. And adding the inoculated dried beancurd stick wastewater into the anode of the fuel cell for treatment.
The specific implementation mode is as follows:
the technical solution of the present invention is further illustrated by the following specific examples:
example 1
The following reagents and consumables are required for treating the dried bean wastewater:
screening Medium
1g/L glucose, 0.158g/L peptone, 0.04g/L beef extract, 0.5g/L yeast powder, 0.13g/L KCl, NaH2PO4·2H2O 3.32g/L，Na2HPO4·12H2O 10.36g/L。
Nfion117 proton semipermeable membrane
Biofuel cell structure
The cell was composed of two square glass containers connected by a flange in the middle, each container having a length of 8cm, a width of 8cm and a height of 8 cm. The middle interface is clamped with a proton semipermeable membrane and sealed by a rubber ring. One chamber is the anode and one chamber is the cathode. The dried bean curd stick waste water is added into the anode, and the deionized water is added into the cathode. During the operation of the cell, air is continuously introduced into the cathode chamber by an air compressor.
The treatment method comprises the following steps:
1. collecting river mud samples, putting the river mud samples into a sterilized collecting pipe, and culturing in an anaerobic incubator by adopting a screening culture medium.
2. After 1 week of anaerobic culture, the microorganisms were isolated. After microscopic examination, rod-shaped microorganisms are selected.
3. Anaerobic culture is carried out by adopting a screening culture medium to separate rod-shaped microorganisms, the rod-shaped microorganisms are cultured for 5 days at 37 ℃, and the rod-shaped microorganisms are inoculated into the bean curd stick wastewater by 6-10% of inoculation amount.
4. And (3) adding the inoculated dried bean curd stick wastewater into the anode of the fuel cell for treatment, wherein in the wastewater treatment process, the open-circuit voltage reaches 1.1V, and the internal resistance is 730 omega.
Through the treatment of the method, the COD (chemical oxygen demand) of the bean curd stick waste water is 16496mg/L, and after 20 days of culture, the lowest COD is measured to be 668 mg/L. The COD removal rate reaches 95.96%, and the COD can be directly discharged into the environment.
Claims (1)
1. A method for treating bean curd stick wastewater by using a microbial battery is characterized by comprising the following steps: 1g/L of glucose, 0.158g/L of peptone, 0.04g/L of beef extract, 0.5g/L of yeast powder, 0.13g/L of KCl and NaH2PO4·2H2O 3.32g/L，Na2HPO4·12H2The method comprises the following steps of taking O10.36 g/L as a screening culture medium, screening electrogenesis microorganisms in river sludge, and treating the dried bean curd stick wastewater by using the screened electrogenesis microorganisms:
1) collecting river mud samples, putting the river mud samples into a sterilized collecting pipe, and culturing in an anaerobic incubator by adopting a screening culture medium;
2) after 1 week of anaerobic culture, separating the microorganisms, and selecting rod-shaped microorganisms after microscopic examination;
3) anaerobic culture is carried out by adopting a screening culture medium to separate rod-shaped microorganisms, the microorganisms are cultured for 5 days at 37 ℃, and the microorganisms are inoculated into the bean curd stick wastewater by 6-10 percent of inoculation amount;
4) adding the inoculated dried bean curd stick wastewater into a fuel cell anode for treatment, wherein in the wastewater treatment process, the open-circuit voltage reaches 1.1V, and the internal resistance is 730 omega; the fuel cell is composed of two square glass containers, the middle of each container is connected by a flange, each container is 8cm long, 8cm wide and 8cm high, a middle interface is clamped with a Nfion117 proton semi-permeable membrane and sealed by a rubber ring, one chamber is an anode, the other chamber is a cathode, the waste water for preparing dried beancurd sticks is added into the anode, and deionized water is added into the cathode; during the operation of the cell, air is continuously introduced into the cathode chamber by an air compressor.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810303589.0A CN108503041B (en) | 2018-03-20 | 2018-03-20 | Method for treating bean curd stick wastewater by using microbial battery |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810303589.0A CN108503041B (en) | 2018-03-20 | 2018-03-20 | Method for treating bean curd stick wastewater by using microbial battery |
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| CN108503041A CN108503041A (en) | 2018-09-07 |
| CN108503041B true CN108503041B (en) | 2021-02-26 |
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| CN117476125B (en) * | 2023-12-27 | 2024-04-05 | 豆黄金食品有限公司 | Dried beancurd stick raffinate recovery data processing system based on data analysis |
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| CN101570731A (en) * | 2009-03-25 | 2009-11-04 | 新奥科技发展有限公司 | Method for domesticating and separating electricigens by electrochemistry |
| CN102324541A (en) * | 2011-07-18 | 2012-01-18 | 北京师范大学 | Microbial fuel cell anode biomembrane functional regulation method |
| CN103937697B (en) * | 2014-01-14 | 2017-01-25 | 暨南大学 | Bacterial strain for degrading dye with high efficiency |
| CN203768095U (en) * | 2014-03-31 | 2014-08-13 | 内蒙古科技大学 | Device for in-situ remediation of polluted groundwater by using microbial electrolysis cell process |
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