CN108498582A - The method for extraction and purification of 9 kinds of anti-oxidant polyphenol in Guava Leaf - Google Patents
The method for extraction and purification of 9 kinds of anti-oxidant polyphenol in Guava Leaf Download PDFInfo
- Publication number
- CN108498582A CN108498582A CN201710113566.9A CN201710113566A CN108498582A CN 108498582 A CN108498582 A CN 108498582A CN 201710113566 A CN201710113566 A CN 201710113566A CN 108498582 A CN108498582 A CN 108498582A
- Authority
- CN
- China
- Prior art keywords
- acid
- kinds
- purification
- guava leaf
- polyphenol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/61—Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Cosmetics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The method for extraction and purification of 9 kinds of anti-oxidant polyphenol belongs to extraction purification technology in Guava Leaf.It includes 9 kinds of anti-oxidant polyphenol in Guava Leaf（Gallic acid, protocatechuic acid, catechin, caffeic acid, chlorogenic acid, ferulic acid, rutin, ellagic acid, Quercetin）Solvent refluxing extraction, purification with macroreticular resin.The present invention is high to the recovery rate of this 9 kinds of anti-oxidant polyphenol of gallic acid, protocatechuic acid, catechin, caffeic acid, chlorogenic acid, ferulic acid, rutin, ellagic acid, Quercetin in Guava Leaf, purification effect is good, and the purity of 9 kinds of anti-oxidant polyphenol is increased to 4 times before purification after purification.This method is easy to operate, can be used for industrialized production, has larger practical significance and wide application prospect.
Description
Technical field
The present invention relates to 9 kinds of anti-oxidant polyphenol in Guava Leaf（Gallic acid, protocatechuic acid, catechin, coffee
Acid, chlorogenic acid, ferulic acid, rutin, ellagic acid, Quercetin）A kind of method for extraction and purification, belong to extraction purification technology.
Background technology
Guava（Psidium guajava L.）For Myrtaceae Psidium evergreen dungarunga or shrub, U.S. is originated in
Continent torrid areas.Guava leaf extract by domestic and foreign scholars be recommended as 15 kinds of most promising " polyphenol " class health products it
One, guava leaf polyphenol is paid close attention to as main active material in Guava Leaf by domestic and foreign scholars.Guava leaf polyphenol
Type is various, and the anti-oxidant polyphenol of the Guava Leaf being currently known has 9 kinds, is gallic acid, protocatechuic acid, catechin, coffee respectively
Coffee acid, chlorogenic acid, ferulic acid, rutin, ellagic acid, Quercetin, they have it is anti-oxidant, remove free radical, be inhibiting bacteria and diminishing inflammation, disease-resistant
It the effects that poison, anticancer, anti-cardiovascular disease, has broad application prospects, and guava is in China Guangdong, Guangxi, Fujian
It is generally planted Deng area, Guava Leaf is at low cost, resource is wide, therefore, studies gallic acid, protocatechuic acid, youngster in Guava Leaf
The extraction and purification process of this 9 kinds of anti-oxidant polyphenol of theine, caffeic acid, chlorogenic acid, ferulic acid, rutin, ellagic acid, Quercetin, it is right
Its medicinal exploitation is of great significance.
Invention content
The present invention provides a kind of anti-oxidant by 9 kinds in the Guava Leaf of solvent refluxing extraction and purification with macroreticular resin
Polyphenol（Gallic acid, protocatechuic acid, catechin, caffeic acid, chlorogenic acid, ferulic acid, rutin, ellagic acid, Quercetin）Extraction
Purification process.
What the present invention adopts the following technical solutions to realize：The extraction purification side of 9 kinds of anti-oxidant polyphenol in Guava Leaf
Method include the solvent refluxing extraction of 9 kinds of anti-oxidant polyphenol, 9 kinds of anti-oxidant polyphenol two steps of purification with macroreticular resin.
Gallic acid, protocatechuic acid, catechin, caffeic acid, chlorogenic acid, ferulic acid, rutin, tan in first step Guava Leaf
Spend the refluxing extraction of acid, Quercetin this 9 kinds of anti-oxidant polyphenol：The Guava Leaf powder of constant weight after crushed is weighed, by material
Liquor ratio 1:The ethanol water that volume fraction is 60%, 3 h of refluxing extraction at 70 DEG C is added in 25 g/mL.Extraction terminates laggard
Row, which filters, removes residue, and filtrate is taken to be concentrated into the 1/5 of original volume on a rotary evaporator to improve target component in extracting solution
Concentration, for use.
Gallic acid, protocatechuic acid, catechin, caffeic acid, chlorogenic acid, ferulic acid, rutin, tan in second step Guava Leaf
Spend the macroporous resin purification of acid, Quercetin this 9 kinds of anti-oxidant polyphenol：Select 11 nonpolar macroporous adsorption resins of ZGDM, use anhydrous
Ethyl alcohol, 5% the HCl of NaOH and 5% pre-treatment is carried out to it, then load pillar（5×25 cm）It is used in combination water as current system
24 h are rinsed to be compacted resin bed.With the flow velocity loading of 5 mL/min, 20 mL/g resins of applied sample amount.It is eluted with 50% acetone,
Elution speed is 5 mL/min, and eluting agent is 5 mL/g resins.Finally 9 kinds of polyphenol components after purification are steamed in rotation
It is concentrated on hair instrument, it is dry on vacuum freeze drier.
The advantages and positive effects of the present invention：The present invention is to gallic acid, protocatechuic acid, catechin, coffee in Guava Leaf
The recovery rate of this 9 kinds of anti-oxidant polyphenol of coffee acid, chlorogenic acid, ferulic acid, rutin, ellagic acid, Quercetin is high, and purification effect is good, pure
The purity of 9 kinds of anti-oxidant polyphenol is increased to 4 times before purification after change.This method is easy to operate, can be used for industrialized production, have
Larger practical significance and wide application prospect.
Description of the drawings
Fig. 1 is the schematic flow diagram of the method for extraction and purification of 9 kinds of anti-oxidant polyphenol in Guava Leaf of the present invention.
Specific implementation mode
Specific embodiment is provided by legend.What legend provided be gallic acid in Guava Leaf, protocatechuic acid, catechin,
The extraction and purification process flow chart of this 9 kinds of anti-oxidant polyphenol of caffeic acid, chlorogenic acid, ferulic acid, rutin, ellagic acid, Quercetin, it
Solvent refluxing extraction including 9 kinds of anti-oxidant polyphenol, 9 kinds of anti-oxidant polyphenol purification with macroreticular resin.
Claims (2)
1. the method for extraction and purification of 9 kinds of anti-oxidant polyphenol in Guava Leaf, it includes gallic acid in Guava Leaf, former catechu
The solvent refluxing of this 9 kinds of anti-oxidant polyphenol of acid, catechin, caffeic acid, chlorogenic acid, ferulic acid, rutin, ellagic acid, Quercetin carries
It takes, two steps of purification with macroreticular resin, it is characterised in that：It is anti-to 9 kinds in Guava Leaf with solvent refluxing extracting method
Oxidation polyphenol extracts, and refluxing extraction condition is solid-liquid ratio 1:25 g/mL, it ethyl alcohol fraction 60%, 70 DEG C of Extracting temperature, carries
Take 3 h of time.
2. gallic acid in Guava Leaf according to claim 1, protocatechuic acid, catechin, caffeic acid, chlorogenic acid, Ah
Wei's acid, rutin, ellagic acid, Quercetin this 9 kinds of anti-oxidant polyphenol extraction and purification process, it is characterised in that：It is non-with ZGDM 11
Polar macroporous adsorption resin purifies 9 kinds of anti-oxidant polyphenol in Guava Leaf.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710113566.9A CN108498582A (en) | 2017-02-28 | 2017-02-28 | The method for extraction and purification of 9 kinds of anti-oxidant polyphenol in Guava Leaf |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710113566.9A CN108498582A (en) | 2017-02-28 | 2017-02-28 | The method for extraction and purification of 9 kinds of anti-oxidant polyphenol in Guava Leaf |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108498582A true CN108498582A (en) | 2018-09-07 |
Family
ID=63373258
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710113566.9A Pending CN108498582A (en) | 2017-02-28 | 2017-02-28 | The method for extraction and purification of 9 kinds of anti-oxidant polyphenol in Guava Leaf |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108498582A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101297865A (en) * | 2008-06-17 | 2008-11-05 | 南方医科大学 | Method from extracting total flavones from guava leaves |
-
2017
- 2017-02-28 CN CN201710113566.9A patent/CN108498582A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101297865A (en) * | 2008-06-17 | 2008-11-05 | 南方医科大学 | Method from extracting total flavones from guava leaves |
Non-Patent Citations (2)
| Title |
|---|
| 杨雪等: "大孔吸附法分离番茄红素的研究", 《药物生物技术》 * |
| 梁清蓉: ""番石榴叶中酚类物质的研究"", 《中国优秀硕士学位论文全文数据库•医药卫生科技辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102702163B (en) | Method for preparing high-purity monomer epigallocatechin gallate from processed leftovers of tea leaves | |
| CN102188501B (en) | Rhubarb anthraquinones component extracting and separating method | |
| CN102746415B (en) | Method for simultaneously extracting tea polysaccharide and tea polyphenol with subcritical water | |
| CN101580525A (en) | Method for extracting salidroside, polysaccharose and tannin-type substances from rhodiola root crude drugs | |
| CN101851163A (en) | Method for extracting high-purity chlorogenic acid from honey suckle flower | |
| CN104906153A (en) | Technological method for efficiently extracting ginkgo flavone | |
| CN101653463A (en) | Extraction and purification method of tannin components in Geranium wilfordii | |
| CN103694364A (en) | Method for synchronously extracting, separating and purifying polysaccharides and flavones of cyclocarya paliurus | |
| CN102895388B (en) | Lychee polyphenol extracts and extraction method thereof | |
| CN103450316B (en) | A kind of method simultaneously extracting tea saponin, tea seed flavonoid glycoside and tea polysaccharide | |
| CN102106877B (en) | Production process of gingko leaf extract | |
| CN101322737B (en) | Persimmon leaf flavones extract and preparation thereof | |
| CN101658290B (en) | Method for extracting crabapple polyphenols from crabapple skin dregs | |
| CN105713053B (en) | A kind of glucosinolate extraction separation method | |
| CN103948654A (en) | Method of extracting, purifying and inhibiting alpha-glucosaccharase active ingredient from chestnut shell | |
| CN105566409B (en) | A method of the extraction separation glucorphanin from broccoli seed | |
| CN103992299B (en) | The method of multiple bioactive ingredients in separation and purification sea-buckthorn seed cake while of a kind of | |
| CN105878306A (en) | Preparation method of pomegranate peel polyphenols | |
| CN103463160A (en) | Preparation method of high-content total flavones of chrysanthemum | |
| CN108498582A (en) | The method for extraction and purification of 9 kinds of anti-oxidant polyphenol in Guava Leaf | |
| CN103385915A (en) | Platycarya fruit extract with anti-oxidization function and preparation method of platycarya fruit extract | |
| CN1962650A (en) | Process for preparing high-purity dehydrated andrographolide | |
| CN103242422A (en) | Method for extracting cyclocaric acid A from cyclocarya paliurus leaves | |
| CN103058882B (en) | Method for separating natural theanine | |
| CN104892696A (en) | Method for extracting salidroside from Tibetan natural rhodiola rosea |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180907 |