CN102106877B - Production process of gingko leaf extract - Google Patents
Production process of gingko leaf extract Download PDFInfo
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- CN102106877B CN102106877B CN2010106152014A CN201010615201A CN102106877B CN 102106877 B CN102106877 B CN 102106877B CN 2010106152014 A CN2010106152014 A CN 2010106152014A CN 201010615201 A CN201010615201 A CN 201010615201A CN 102106877 B CN102106877 B CN 102106877B
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Abstract
The invention relates to a production process of a gingko leaf extract, belonging to the refining field of natural plant extracts. The production process comprises the following steps: (1) extraction: purifying gingko leaves and extracting in a whole leaf manner, wherein ethanol is used as an extracting agent, the leaves are extracted for three times in each round, the extract liquor obtained after the last two extractions are combined to be used as a solvent for the first time in the second round, a new solvent is used in the second and third extractions in the second round, and the extract liquor obtained after the last two extractions in the second round is used as the primary solvent for the first time in the third round, and the steps are repeated; (2) concentration: concentrating the first material liquid in each turn at reduced pressure until the liquid does not contain alcohol, and adding water to dilute the liquid; (3) sedimentation: adding an enzyme preparation, and standinguntil a precipitate is generated; (4) centrifuging; (5) resin treatment; (6) concentrating so as to obtain gingko leaf extract desorption concentrated liquid; and (7) spray drying and sterilizing, soas to obtain a low acid powdered gingko leaf extract. The process is simple, the cost is low, and the use amount of the solvent is greatly reduced.
Description
Technical field
The present invention relates to a kind of production technology of Folium Ginkgo extract, belong to the natural plant extracts field of refinement.
Background technology
China is the original producton location of Semen Ginkgo, is again the main producing region of world Semen Ginkgo, and China's gingko resource accounts for 70% of the world at present.The effective ingredient that from Folium Ginkgo, extracts mainly is a ginkgetin etc., and it has the cardiovascular and cerebrovascular circulation of improvement Semen Ginkgo pyrite, the treatment cardiovascular and cerebrovascular disease, and improve effects such as memory, slow down aging.Ginkgetin extraction process and ginkgoic acid content have determined Semen Ginkgo product quality and price.
At present; The research of from Folium Ginkgo, extracting ginkgetin is more, and it is big that still there is the use quantity of solvent mostly in existing extraction process, feed liquid concentrates amount, and the solvent ratios that is widely used in the Folium Ginkgo extraction at present is more than 10 times; Seriously restricted the development that Folium Ginkgo extracts; In this case, take traditional production process, tend to cause shortcomings such as inventory is few when extracting, extraction solvent use amount is big, the concentrated amount of extracting solution is big.Extracting solvent can not be by abundant use, and flavonoid of ginkgo biloba is saturated inadequately in the extracting solution, and the use of extraction solvent is insufficient, causes extraction solvent use amount big, and feed liquid concentrates amount greatly, has closely caused the production cost height, and energy consumption increases.
There is the problem of ginkgoic acid too high levels mostly in the ginkgetin that existing extraction process obtains, and ginkgoic acid can not be contained a large amount of ginkgoic acids by abundant removal in the flavone product, have a strong impact on product quality.
Summary of the invention
In order to overcome the shortcoming of prior art, the present invention provides a kind of production technology of Folium Ginkgo extract, and its technology is simple, and cost is low, can significantly reduce the use amount of solvent.
The present invention solves the technical scheme that its technical problem takes: may further comprise the steps: (1), extraction: with the Folium Ginkgo remove impurity, and whole leaf extraction; Ethanol with 60-70% concentration is made extractant; Solid-liquid ratio: be for the 1st time be for 1: 6, the 2nd time 1: 3, the 3rd time be 1: 3; The extract that the back is twice merges the 1st time use of taking turns as second; Use novel solvent second take turns the 2nd time and the 3rd time, so circulation is used in the 1st time of merging as third round of second back twice extract of taking turns; (2), concentrate: each the 1st time feed liquid of taking turns is evaporated to soft, and thin up; (3), deposition: add enzyme preparation, room temperature staticly settles; (4), centrifugal: it is centrifugal with centrifuge to precipitate good supernatant, gets centrifugal liquid; (5), resin treatment: gained centrifugal liquid is used macroporous resin adsorption, resolve as resolving solvent, obtain the alcoholic solution of Folium Ginkgo extract with 70-80% ethanol; (6), concentrate: it is 10-20% that the alcoholic solution of gained Folium Ginkgo extract is concentrated into solid content, Folium Ginkgo extract desorbing concentrated solution; (7) spray drying: will concentrate the sterilization of gained Folium Ginkgo extract desorbing concentrated solution, and carry out spray drying, and must hang down sour Powdered Folium Ginkgo extract, wherein the ginkgetin volume content is 24%, and ginkgoic acid is less than 1ppm.
Enzyme preparation in the said step (3) is a kind of pectase, cellulase, protease and diastatic compound enzymic preparation of comprising.
Said pectase, cellulase, protease and diastatic volume ratio are 2: 1: 1: 2.
The present invention recycles through extractant, has reduced the use amount of solvent, has reduced the concentrated amount of extractant, has reduced production cost, and whole production technology is simple, and cost is low, is fit to suitability for industrialized production.Enzyme preparation used in the present invention is a kind of natural clarifying agent material, can significantly reduce ginkgoic acid content behind the adding clarifier, plays fine purification.Gained powder ginkgetin unit content is 24%, and ginkgoic acid is less than 1ppm.
The specific embodiment
Case study on implementation one
(1), the every wheel get 30 kg silver Folium Pruni raw materials, in alcoholic solution, extract.Extract is: 70% ethanol; Solid-liquid ratio: be for the 1st time be for 1: 6, the 2nd time 1: 3, the 3rd time be 1: 3; The extract that the back is twice merges the 1st time use of taking turns as second; Use novel solvent second take turns the 2nd time and the 3rd time; The 1st time of merging as third round of second back twice extract of taking turns used then, circulates so successively; Extraction temperature first pass extraction temperature is 70 ℃, and second time extraction temperature is 65 ℃, and the 3rd time extraction temperature is 65 ℃; The extraction time is: first pass 2 hours, second time 1 hour, the 3rd time 1 hour.(2), get and respectively take turns the first pass extract, utilize membrane evaporator be evaporated to soft till, add water to 100L.(3), deposition: add enzyme preparation 15g, room temperature staticly settled 2 hours.(4), centrifugal: as will to precipitate good supernatant and utilize disk plate centrifuge centrifugal, and get centrifugal liquid.(5), resulting Folium Ginkgo extract centrifugal liquid is carried out macroporous resin adsorption, wash and control dried earlier, utilize 80% ethanol to resolve then.Adsorption rate is 0.12m
3/ hour, washing speed 0.22m
3/ hour, resolution speed 0.12m
3/ hour.(6), use membrane evaporator that desorbed solution is concentrated into solid content and be 15%, the Folium Ginkgo extract concentrated solution is sterilized that spray drying obtains powdery Folium Ginkgo extract product then.The Folium Ginkgo extract quality of three-wheel is 0.909kg, and flavonoid of ginkgo biloba is 27.5%, ginkgoic acid 0.7ppm.
Embodiment two
(1), the every wheel get 60 kg silver Folium Pruni raw materials, in alcoholic solution, extract.Extract is 70% ethanol; Solid-liquid ratio: be for the 1st time be for 1: 6, the 2nd time 1: 3, the 3rd time be 1: 3; The extract that the back is twice merges the 1st time use of taking turns as second; Use novel solvent second take turns the 2nd time and the 3rd time; The 1st time of merging as third round of second back twice extract of taking turns used then, circulates so successively.Extraction temperature first pass extraction temperature is 70 ℃, and second time extraction temperature is 65 ℃, and the 3rd time extraction temperature is 65 ℃; The extraction time is first pass 2 hours, second time 1 hour, and the 3rd time 1 hour.(2), get and respectively take turns the first pass extract, utilize membrane evaporator be evaporated to soft till, add water to 200L.(3), deposition: add enzyme preparation 30g, room temperature staticly settles 2h.(4), centrifugal: as will to precipitate good supernatant and utilize centrifuge centrifugal, and get centrifugal liquid.(5), resulting Folium Ginkgo extract centrifugal liquid is carried out macroporous resin adsorption, wash and control dried earlier, utilize 80% ethanol to resolve then.Adsorption rate is 0.09m
3/ hour, washing speed 0.2m
3/ hour, resolution speed 0.15m
3/ hour.(6), use membrane evaporator that desorbed solution is concentrated into solid content 15%, the Folium Ginkgo extract concentrated solution is sterilized, spray drying obtains powdery Folium Ginkgo extract product then.The Folium Ginkgo extract quality of three-wheel is 1.758kg, and flavonoid of ginkgo biloba is 26.3%, ginkgoic acid 0.8ppm.
Claims (1)
1. the production technology of a Folium Ginkgo extract is characterized in that: may further comprise the steps: (1), extraction: with the Folium Ginkgo remove impurity, put in order the leaf extraction; Ethanol with 60-70% concentration is made extractant; Solid-liquid ratio: be for the 1st time be for 1: 6, the 2nd time 1: 3, the 3rd time be 1: 3; The extract that the back is twice merges the 1st time use of taking turns as second; Use novel solvent second take turns the 2nd time and the 3rd time, so circulation is used in the 1st time of merging as third round of second back twice extract of taking turns; (2), concentrate: each the 1st time feed liquid of taking turns is evaporated to soft, and thin up; (3), deposition: add enzyme preparation, room temperature staticly settles, and described enzyme preparation is pectase, cellulase, protease and diastatic compound enzymic preparation, and said pectase, cellulase, protease and diastatic volume ratio are 2: 1: 1: 2; (4), centrifugal: it is centrifugal with centrifuge to precipitate good supernatant, gets centrifugal liquid; (5), resin treatment: gained centrifugal liquid is used macroporous resin adsorption, resolve as resolving solvent, obtain the alcoholic solution of Folium Ginkgo extract with 70-80% ethanol; (6), concentrate: it is 10-20% that the alcoholic solution of gained Folium Ginkgo extract is concentrated into solid content, Folium Ginkgo extract desorbing concentrated solution; (7) spray drying: will concentrate the sterilization of gained Folium Ginkgo extract desorbing concentrated solution, and carry out spray drying, and must hang down sour Powdered Folium Ginkgo extract, wherein the ginkgetin volume content is 24%, and ginkgoic acid is less than 1ppm.
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| CN2010106152014A CN102106877B (en) | 2010-12-22 | 2010-12-22 | Production process of gingko leaf extract |
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| CN2010106152014A CN102106877B (en) | 2010-12-22 | 2010-12-22 | Production process of gingko leaf extract |
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| CN102106877B true CN102106877B (en) | 2012-05-23 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102626154A (en) * | 2012-04-11 | 2012-08-08 | 徐州绿之野生物食品有限公司 | Ginkgo leaf extraction technology and processing method of ginkgo yellow tea |
| CN102728096B (en) * | 2012-07-10 | 2014-06-11 | 江苏惠康生物有限公司 | Edulcoration method of ginkgo leaf extraction process |
| CN103638071A (en) * | 2013-12-23 | 2014-03-19 | 湖南科技学院 | Efficient production method of gingko medicinal extract |
| CN106811303B (en) * | 2015-12-02 | 2020-04-03 | 中国科学院大连化学物理研究所 | Method for simultaneously extracting essential oil and pectin from ginkgo biloba sarcotesta |
| CN105560301B (en) * | 2015-12-29 | 2019-08-20 | 晨光生物科技集团股份有限公司 | A kind of ginkgo biloba p.e large-scale method for producing |
| CN114570057A (en) * | 2022-03-07 | 2022-06-03 | 郑州市金色农业科技发展有限公司 | Method for extracting inhibitor from multi-element substance |
| CN114907896A (en) * | 2022-04-27 | 2022-08-16 | 南京林业大学 | Ginkgo leaf enzymolysis treatment method and application thereof |
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| CN101703533A (en) * | 2009-09-29 | 2010-05-12 | 武征杰 | Ginkgo ganoderma lucidum spore capsule and preparation method thereof |
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