CN108478861B - Needle head used for 3D printing and method for 3D printing of cylindrical single fiber with petal-shaped groove - Google Patents
Needle head used for 3D printing and method for 3D printing of cylindrical single fiber with petal-shaped groove Download PDFInfo
- Publication number
- CN108478861B CN108478861B CN201810383703.5A CN201810383703A CN108478861B CN 108478861 B CN108478861 B CN 108478861B CN 201810383703 A CN201810383703 A CN 201810383703A CN 108478861 B CN108478861 B CN 108478861B
- Authority
- CN
- China
- Prior art keywords
- printing
- petal
- needle head
- needle
- grooves
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/12—Phosphorus-containing materials, e.g. apatite
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y10/00—Processes of additive manufacturing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y30/00—Apparatus for additive manufacturing; Details thereof or accessories therefor
Abstract
The invention discloses a needle head for 3D printing, which structurally comprises petal-shaped grooves, wherein the petal-shaped grooves are formed by connecting semicircular grooves in series, the radius of each semicircular groove is 5-20 mu m, the diameter of an external circle of the needle head is 100-1000 mu m, the diameter of an internal circle of the needle head is 80-980 mu m, and cylindrical single fibers with petal-shaped grooves are printed by the needle head in a 3D mode. The invention also discloses a method for 3D printing of cylindrical single fibers with petal grooves by using the needle head. The fiber scaffold printed by the method has better capacity of promoting cell adhesion and migration and more controllable capacity of promoting cells to differentiate towards a specific direction.
Description
Technical Field
The invention belongs to the field of 3D printing and tissue engineering, and particularly relates to a needle used for 3D printing and a method for 3D printing of a cylindrical single fiber with a petal groove.
Background
The construction of animal tissues is a result of both multicellular interactions and interactions between cells and the extracellular matrix. The mechanical stress, intercellular adhesion and cytokine on the cell are all combined to regulate the cell behavior. For example, numerous studies have demonstrated that micro-nano structures (e.g., grooves) of the extracellular matrix can affect cell migration and differentiation. In tissue engineering, the main function of a scaffold is to simulate extracellular matrix and provide nutrients and mechanical support for the growth of cells. There are many manufacturing methods for scaffolds, and among them, 3D printing technology has been widely used for the construction of scaffolds for tissue engineering due to its advantages of rapidity, accuracy, etc. There are many types of 3D printing including melt extrusion, ink jet printing, selective laser sintering, photocuring, and the like. Among them, melt extrusion is widely used for reasons of simple maintenance, low cost, and the like. In this process, the material is heated and melted in the nozzle. The nozzle moves along the cross-sectional profile and filling trajectory of the part while extruding the molten material, which solidifies rapidly and bonds with the surrounding material. However, fibers extruded by the melt extrusion process are all slender and cylindrical, and cells can only feel a two-dimensional planar structure, but cannot regulate cell behaviors in a three-dimensional scale.
Disclosure of Invention
The invention provides a needle head for 3D printing, and provides a method for 3D printing of cylindrical single fibers with petal grooves.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a needle head used for 3D printing comprises petal-shaped grooves, wherein the petal-shaped grooves are formed by connecting semicircular grooves in series, and the radius of each semicircular groove is 5-20 micrometers; the diameter of an external circle of the needle head is 100-1000 microns, the diameter of an internal circle of the needle head is 80-980 microns, and cylindrical single fibers with petal grooves are printed out by the needle head in a 3D mode.
Preferably, the length of the needle is between 2000 and 3000 μm.
Preferably, the material of the needle is a material which is easily pressed and deformed.
Preferably, the easily press-deformable material is stainless steel.
A method for 3D printing of cylindrical single fibers with petal grooves uses a printing material to print the fibers through any needle head.
Preferably, the marking material is hydroxyapatite.
Preferably, the printing material is a polylactic acid-glycolic acid copolymer.
Preferably, the pressure of the process is between 0.2MPa and 0.4 MPa.
Preferably, the temperature of the process is from 20 ℃ to 80 ℃.
Advantages and advantageous effects of the invention
(1) The invention enables the surface of the bracket fiber manufactured by the 3D printing technology to have an accurate micro-nano structure. Particularly, a groove structure can be constructed on the surface of the 3D printed fiber, and compared with a common scaffold under the same condition, the groove structure has better capability of promoting cell adhesion and migration and more controllable capability of promoting cells to differentiate towards a specific direction, so that a more accurate and effective design scheme is provided for tissue engineering.
(2) The invention is used for solving the problem that the traditional 3D printing technology can not construct a micro-nano structure on the surface of a single fiber but can only regulate and control cells on a two-dimensional plane.
Drawings
FIG. 1 is a sectional view of a needle of example 1.
Fig. 2 is a side perspective view of the needle of the present invention.
Fig. 3 is a full perspective view of the needle of the present invention.
Fig. 4 is a cross-sectional view of a 3D printed single filament according to the present invention.
Fig. 5 is a top view of a needle-printed hydroxyapatite scaffold of the present invention.
Fig. 6 is a laser confocal cell diagram of the hydroxyapatite scaffold surface seeded with human mesenchymal stem cells.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1
The utility model provides a 3D prints syringe needle of use, the syringe needle structure includes petal recess, petal recess is established ties by semicircular groove and is constituteed, semicircular groove's radius is 20 μm, the circumscribed circle diameter of syringe needle is 1000 μm, the inscribed circle diameter of syringe needle is 980 μm, syringe needle 3D prints out the single fibre of cylinder that has the petal recess.
The length of the needle is 3000 μm. The material of the needle is stainless steel.
A method for 3D printing of cylindrical single fibers with petal grooves is used for printing the fibers by using the needle head. The printing material is hydroxyapatite. The pressure of the method is 0.4Mpa, and the printing temperature is room temperature.
Orthogonal hydroxyapatite scaffolds of 1cm × 1cm × 3mm were printed according to the above parameters, with a scaffold layer spacing of 900 μm and a fiber spacing of 800 μm. After high-temperature sintering, the scaffold is sterilized at high temperature and high pressure, placed in a 48-hole plate, and inoculated with human mesenchymal stem cells (hBMSC). The amount of each scaffold inoculated was 30 w. When inoculating, firstly dripping 50 microliter of high-concentration cell suspension, and after the cells are spread by adherence for one hour, supplementing 500 microliter of culture medium into each hole. After 24 hours, confocal laser observation was used and photographs were taken. The above-mentioned hbmscs are autofluorescent cells, and therefore, direct observation without staining was sufficient.
FIG. 6 is a confocal laser photograph showing that, as shown in FIG. 6, the cells seeded on the surface of the stent spread in the groove structure and are elongated in order. Indicating that the groove structure has an influence on the skeletal structure of the cells. Compared with the common scaffold under the same condition, the scaffold has better capability of promoting cell adhesion and migration and more controllable capability of promoting cells to differentiate towards a specific direction, thereby providing a more accurate and effective design scheme for tissue engineering.
Example 2
The utility model provides a 3D prints syringe needle of use, the syringe needle structure includes petal recess, petal recess is established ties by semicircular groove and is constituteed, semicircular groove's radius is 20 μm, the circumscribed circle diameter of syringe needle is 400 μm, the inscribed circle diameter of syringe needle is 380 μm, syringe needle 3D prints out the single fibre of cylinder that has the petal recess.
The length of the needle is 2000 μm. The material of the needle is stainless steel.
A method for 3D printing of cylindrical single fibers with petal grooves is used for printing the fibers by using the needle head. The printing material is polylactic-co-glycolic acid (PLGA). The pressure of the method is 0.2MPa, and the printing temperature is 80 ℃.
The present invention is not limited to the above embodiments, and various other modifications, substitutions and alterations can be made without departing from the basic technical concept of the present invention by the common technical knowledge and conventional means in the field according to the above content of the present invention.
Claims (9)
1. The utility model provides a syringe needle that 3D printed and used which characterized in that: the needle head structure comprises petal-shaped grooves, the petal-shaped grooves are formed by connecting semicircular grooves in series, and the radius of the semicircular grooves is 5-20 mu m; the diameter of an external circle of the needle head is 100-1000 microns, the diameter of an internal circle of the needle head is 80-980 microns, and cylindrical single fibers with petal grooves are printed out by the needle head in a 3D mode.
2. The needle of claim 1, wherein: the length of the needle head is 2000-3000 μm.
3. The needle of claim 1, wherein: the material of the needle head is a material easy to press and deform.
4. A needle according to claim 3, wherein: the easily pressed and deformed material is stainless steel.
5. A3D printing method for cylindrical single fibers with petal grooves is characterized by comprising the following steps: printing fibers through the needle of any of claims 1 to 4 using a printing material.
6. The method of claim 5, wherein: the printing material is hydroxyapatite.
7. The method of claim 5, wherein: the printing material is polylactic acid-glycolic acid copolymer.
8. The method of claim 5, wherein: the pressure of the method is 0.2MPa to 0.4 MPa.
9. The method of claim 5, wherein: the temperature of the method is 20-80 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810383703.5A CN108478861B (en) | 2018-04-26 | 2018-04-26 | Needle head used for 3D printing and method for 3D printing of cylindrical single fiber with petal-shaped groove |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810383703.5A CN108478861B (en) | 2018-04-26 | 2018-04-26 | Needle head used for 3D printing and method for 3D printing of cylindrical single fiber with petal-shaped groove |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108478861A CN108478861A (en) | 2018-09-04 |
| CN108478861B true CN108478861B (en) | 2021-03-30 |
Family
ID=63314271
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810383703.5A Active CN108478861B (en) | 2018-04-26 | 2018-04-26 | Needle head used for 3D printing and method for 3D printing of cylindrical single fiber with petal-shaped groove |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108478861B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103451751A (en) * | 2013-08-02 | 2013-12-18 | 北京化工大学 | Electrospinning device with bi-layer petal-shaped nozzle |
| CN107000316A (en) * | 2014-11-27 | 2017-08-01 | 飞利浦照明控股有限公司 | Printhead, printing equipment, Method of printing and the article of printing |
| CN107127963A (en) * | 2017-07-01 | 2017-09-05 | 六安永贞匠道机电科技有限公司 | The adjustable 3D printer shower nozzle of bore |
| CN107150443A (en) * | 2017-07-01 | 2017-09-12 | 六安永贞匠道机电科技有限公司 | Print the whole 3D printer shower nozzle of variable area |
| CN107415248A (en) * | 2017-08-03 | 2017-12-01 | 金华市瑞意电气科技有限公司 | A kind of melting control device and method of 3D printing |
| KR20180041596A (en) * | 2016-10-14 | 2018-04-24 | 한양대학교 에리카산학협력단 | Dispenser and Operating Method thereof |
-
2018
- 2018-04-26 CN CN201810383703.5A patent/CN108478861B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103451751A (en) * | 2013-08-02 | 2013-12-18 | 北京化工大学 | Electrospinning device with bi-layer petal-shaped nozzle |
| CN107000316A (en) * | 2014-11-27 | 2017-08-01 | 飞利浦照明控股有限公司 | Printhead, printing equipment, Method of printing and the article of printing |
| KR20180041596A (en) * | 2016-10-14 | 2018-04-24 | 한양대학교 에리카산학협력단 | Dispenser and Operating Method thereof |
| CN107127963A (en) * | 2017-07-01 | 2017-09-05 | 六安永贞匠道机电科技有限公司 | The adjustable 3D printer shower nozzle of bore |
| CN107150443A (en) * | 2017-07-01 | 2017-09-12 | 六安永贞匠道机电科技有限公司 | Print the whole 3D printer shower nozzle of variable area |
| CN107415248A (en) * | 2017-08-03 | 2017-12-01 | 金华市瑞意电气科技有限公司 | A kind of melting control device and method of 3D printing |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108478861A (en) | 2018-09-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kade et al. | Polymers for melt electrowriting | |
| Ozbolat et al. | Development of ‘Multi-arm Bioprinter’for hybrid biofabrication of tissue engineering constructs | |
| CN104888277B (en) | A kind of cell biological support complex and its 3D printing manufacturing process | |
| Smith et al. | Characterizing environmental factors that impact the viability of tissue-engineered constructs fabricated by a direct-write bioassembly tool | |
| CN104548208B (en) | A kind of preparation method and application of load cells three-dimensional rack | |
| US10350329B2 (en) | Graphene-based ink compositions for three-dimensional printing applications | |
| Chen et al. | Converting 2D nanofiber membranes to 3D hierarchical assemblies with structural and compositional gradients regulates cell behavior | |
| CN104490489B (en) | Method for preparing tissue engineering blood vessel based on 3D bioprinting technology | |
| CN106110401A (en) | Micro-nano composite double layer dermal scaffold and preparation method thereof | |
| CN106916781A (en) | A kind of construction method of external 3 D human body hepatic tissue and its application | |
| CN210612586U (en) | Anti-inflammatory meniscus support loaded with chondrocytes | |
| CN101680011A (en) | Be used to prepare the method for the structure that comprises crystalline cellulose | |
| JP2018516619A5 (en) | ||
| CN102553064A (en) | Needle array transdermal absorption sheet and method for manufacturing needle array transdermal absorption sheet | |
| CN104844814A (en) | Microneedle template and preparation method thereof | |
| CN108478861B (en) | Needle head used for 3D printing and method for 3D printing of cylindrical single fiber with petal-shaped groove | |
| CN113215101B (en) | Method for constructing fiber bundle/tissue structure with heterogeneous functional fibers and blood vessel channel | |
| CN206809488U (en) | A kind of cytoskeleton preparation system | |
| CN105664262A (en) | Method for preparing three-dimensional interconnected blood vessel-liked structure network scaffold by roller type collection | |
| CN104931683A (en) | Myocardial tissue sensor and myocardial tissue chip preparation method | |
| CN108619570A (en) | A kind of artificial organ organ soft bracket preparation method that growth factor is controllable | |
| Desai et al. | Understanding microdroplet formations for biomedical applications | |
| Kornikova et al. | Relocations of cell convergence sites and formation of pharyngula-like shapes in mechanically relaxed Xenopus embryos | |
| KR101566810B1 (en) | Air jet scaffold manufacturing device and manufacturing method for using the same | |
| CN104419696A (en) | Three-dimensional controllable patterning method for cancer cell spheres |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |